GPR158 cDNA ORF clone, Canis lupus familiaris(Dog)

The following GPR158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPR158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe35130 XM_014121676.2
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Canis lupus familiaris G protein-coupled receptor 158 (GPR158), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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OCe35130 XM_014121676.1 Canis lupus familiaris G protein-coupled receptor 158 (GPR158), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe35130
    Clone ID Related Accession (Same CDS sequence) XM_014121676.2 , XM_014121676.1
    Accession Version XM_014121676.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable G-protein coupled receptor 158
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCCA TGGCTTATCC CTTACTCTTC TGCCTCCTGC TCGCTCATCT GGGTTTGGGA 
    ACTGTTAGCA CCAGCCGCGA CCCTCCGGGA CGGCTGGATT CCCCTCGAGA GAGAAGCCTG
    AGGCTGAAGC AGCACGCCCA GCAGCCCGCG CGAGCCGCTG CCTCGGACCC CTCGGCTCCC
    TGGAGCCGCT CCACCGACGG CACCATCTTG GCGCAGAAAC TCGCCGAGGA GGTGCCCATG
    GACGTGGCCT CTTACCTCTA CACCGGGGAC TCCCACCAGC TGAAGCGAGC CAACTGCTCC
    GGCCGCTACG AGTTGGCGGG CCTGCCGGGG AAGTCGTCGG CCCTGGCCAG CTCCCACCCC
    TCCTTGCACG GGGCGCTGGA CACGCTGACA CACGCCACTA ACTTCCTCAA CATGATGCTG
    CAGAGCAATA AGTCGCGGGA GCAGAACTTG CAGGACGACC TGGAGTGGTA CCAGGCACTG
    GTGCGGAGTC TGCTGGAGGG CGAGCCCAGC ATTTCCCGGG CAGCCATCAC CTTCAGCACC
    GAGTCGCTGT CCGCGCCCGC CCCGCAGGTT TTCCTCCAGG CCACCCGCGA GGAGAGTCGC
    ATCCTGCTCC AGGACCTGTC CTCCTCGGCA CACCACCTGG CCAACGCCAC GCTGGAGACC
    GAGTGGTTCC ACGGCCTGCG GCGCAAGTGG CGGACCCACT TACACCGCCG CGGCTCTAAC
    CAGGGGCCCC GGAGCCTGGG CCATAGCTGG CGGCGCCGGG ACGGGCTTGG CGGGGACAAG
    AGCCATGTCA AGTGGTCCCC GCCTTATTTG GAGTGCGAGA ACGGGAGTTA CAAGCCTGGG
    TGGCTGGTCA CTCTCTCCGC AGCTTTCTAC GGGTTGCAGC CTAACCTGGT CCCCGAATTC
    AGGGGTGTTA TGAAAGTTGA CATAAATCTT CAGAAAGTGG ACATTGACCA ATGTTCAAGT
    GATGGCTGGT TTTCAGGAAC TCACAAATGT CACCTTAACA ATTCAGAGTG TATGCCAATT
    AAAGGCCTAG GATTCGTGCT TGGAGCCTAC CAGTGCATTT GCAAAGCAGG ATTCTATCAT
    CCTCAAGACT TCTCAGTGAA CAGCTTTCAG AGAAGTGCAA AAGATGTGTC AGAAGAAGTC
    CATGTCTGCC TACCCTGCAG GGAGGGCTGC CCCTACTGTG CTGATGACAG CCCCTGCTTT
    GTCCAGGAGG ATAAGTATTT GCGACTTGCT ATCATCTCCT TCCAAGCCCT GTGTATGCTG
    CTTGACTTCG TTAGCATGCT GGTGGTCTAC CACTTTCGCA AAGCAAAGAG CATCAGGGCA
    TCAGGCCTTA TCCTGTTGGA AACAATTCTT TTTGGGTCTC TGCTTCTATA CTTTCCTGTT
    GTTATTTTGT ATTTTGAGCC AAGCACATTT CGCTGTATTC TCCTAAGATG GGTCCGTCTT
    CTCGGTTTTG CTACTGTTTA CGGAACTGTC ACTCTCAAGC TTCACAGGGT TTTGAAGGTG
    TTTCTTTCAC GAACAGCCCA GCGGATTCCA TATATGACTG GTGGACGGGT CATGAGGATG
    CTGGCAGTAA TGCTCCTGGT AGTATTTTGG TTTCTTGTTG GCTGGACTTC GTCTGTTTGC
    CAGAATTTGG AGAGGCAGAT TTCACTTATT GGCCAAGGGC AAACATCTGA TCACCTCATC
    TTCAATATGT GCCTCATTGA GCGCTGGGAT TACATGACAG CAGTTGCTGA ATTTTTATTC
    CTCTTGTGGG GTGTTTATCT CTGCTATGCA GTGCGGACCG TCCCATCAGC ATTTCATGAG
    CCACGATATA TGGCTGTTGC AGTTCACAAT GAGCTCATTA TCTCTGCTAT ATTCCATACA
    ATTAGATTCG TTCTTGCCTC AAGACTTCAG TCTGACTGGA TGCTAATGCT GTATTTTGCA
    CATACTCATT TGACTGTGAC AGTCACCATT GGGTTGCTTT TGATTCCAAA GTTTTCACAT
    TCAAGCAATA ACCCCCGAGA TGACATTGCT ACAGAAGCAT ATGAAGATGA GCTAGACCTG
    GGTAGGTCTG GGTCCTACCT GAACAGCAGC ATCAACTCTG CATGGAGTGA GCACAGCTTG
    GACCCAGAGG ACATTCGGGA TGAGCTAAAG AAACTCTATG CCCAGTTGGA AATATATAAA
    AGGAAGAAGA TGATTACAAA TAACCCTCAC CTCCAGAAAA AGCGGTGCTC CAAGAAGGGC
    TTAGGCCGTT CCATCATGAG GCGTATAACC GAGATCCCAG AGACGGTTAG CAGGCAGTGT
    TCCAAAGAGG ACAAGGAAGT CACAGACCAC AGCACAGCCA AAAGCACTGC CCTGGCCAGG
    AAGAACCCAC AAGAATCTTC AGGTAACACC GGGAAGCCCA AAGAAGAGTC CCTGAAAAAC
    CGAGTCTTCT CGCTAAAGAA ATCACACAGC ACTTATGATC ATGTGAGGGA GCACACGGAA
    GAGTCCAACA GTTTACCAGT GGAAAGCCAA GATGAGGAGG CTACAGAACA TTCCACACTA
    GAGTCTCTGT CAAGTAAAAA ACTAACACAA AAACTGAAAG AAAACAGTGA GGCCGAGTCC
    ACAGAGTCCG TGCCTTTGGT GTGTAAGTCA GCAAGTGCAC ACAATCTCAG CTCAGAGAAG
    AAGGCAGGCC ACCCCCGAAC ATCGATGTTG CAAAAGTCTC TCAGTGTCAT CGCAAGCGCC
    AAGGAGAAGA CTCTTGGATT AGCTGGGAAA ACTCAAACAT CAGGCATGGA AGAACGTGCT
    AAAGCCCAGA AACCAAAAGA TAAAGAAACA AATAGAAAAT ACTCAAATTC AGATAATGCA
    GAGACTAATG ATTCTGCCCC CCAAAACTCC AATCATTTGG AGGAGCCAAG AAAACCTCAG
    AAATCTGGGA TTATGAAGCA ACAACGGGTC AACCCGGCCA CTGCCAATTC TGACATGAGC
    CCAGGCACCC CCCAGACGAA GAACAACTTT GACATTGGGG AAGTGTGCCC TTGGGAGATT
    TATGACCTGG CCCCCGGTCC TGTGCCTTCA GAATCAAAAG TTCAAAAACA CGTATCTATT
    GCAGCTTCTG AAATGGAGAA AAACCCAACT TTTTCCTTAA AGGAGAAGTC TCATCAGAAG
    CCCAAGGCAG CTGAACTGTG TCAGCAAACC AATCAGAAGA GCATAGACAA GGCTGAGGTG
    TGCCCTTGGA AGAGCCAAGG CCAGCCTGGT TTTGAAGATG AGAAGCATCT GATTCCCAAG
    ACTCAAGTTC CCGCAGGGAG AGCCAGAGAG GAGAATGGCA ATCAGCATTA TGCAGCCAAT
    GTGTGTGCCG GGCAGTATGA AGAACTGCCC CCCAAAGCTA TAGCATCAAA AGTCGAGAAT
    GAAAGTCTCA ACCAAACAGG AGACCAGGAG AAAAAGACAT CCTCCCCTGA GGAAAATGTG
    CCTGGCTCCT ATAACTCAAG TAATAACTTT CGGCAGCCTT TGACATCACG AGCTGAGGTG
    TGTCCTTGGG AGTTTGAGAC CCCAAATCAA CCAAATGCTG AAAGAAGTGT AGCTTTACCT
    GCCTCTTCCA CTTTAAGTGC AAATAAAATA GCAGGGCCTC GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013977151.1
    CDS1460..5047
    Translation

    Target ORF information:

    RefSeq Version XM_014121676.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris G protein-coupled receptor 158 (GPR158), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121676.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCCA TGGCTTATCC CTTACTCTTC TGCCTCCTGC TCGCTCATCT GGGTTTGGGA 
    ACTGTTAGCA CCAGCCGCGA CCCTCCGGGA CGGCTGGATT CCCCTCGAGA GAGAAGCCTG
    AGGCTGAAGC AGCACGCCCA GCAGCCCGCG CGAGCCGCTG CCTCGGACCC CTCGGCTCCC
    TGGAGCCGCT CCACCGACGG CACCATCTTG GCGCAGAAAC TCGCCGAGGA GGTGCCCATG
    GACGTGGCCT CTTACCTCTA CACCGGGGAC TCCCACCAGC TGAAGCGAGC CAACTGCTCC
    GGCCGCTACG AGTTGGCGGG CCTGCCGGGG AAGTCGTCGG CCCTGGCCAG CTCCCACCCC
    TCCTTGCACG GGGCGCTGGA CACGCTGACA CACGCCACTA ACTTCCTCAA CATGATGCTG
    CAGAGCAATA AGTCGCGGGA GCAGAACTTG CAGGACGACC TGGAGTGGTA CCAGGCACTG
    GTGCGGAGTC TGCTGGAGGG CGAGCCCAGC ATTTCCCGGG CAGCCATCAC CTTCAGCACC
    GAGTCGCTGT CCGCGCCCGC CCCGCAGGTT TTCCTCCAGG CCACCCGCGA GGAGAGTCGC
    ATCCTGCTCC AGGACCTGTC CTCCTCGGCA CACCACCTGG CCAACGCCAC GCTGGAGACC
    GAGTGGTTCC ACGGCCTGCG GCGCAAGTGG CGGACCCACT TACACCGCCG CGGCTCTAAC
    CAGGGGCCCC GGAGCCTGGG CCATAGCTGG CGGCGCCGGG ACGGGCTTGG CGGGGACAAG
    AGCCATGTCA AGTGGTCCCC GCCTTATTTG GAGTGCGAGA ACGGGAGTTA CAAGCCTGGG
    TGGCTGGTCA CTCTCTCCGC AGCTTTCTAC GGGTTGCAGC CTAACCTGGT CCCCGAATTC
    AGGGGTGTTA TGAAAGTTGA CATAAATCTT CAGAAAGTGG ACATTGACCA ATGTTCAAGT
    GATGGCTGGT TTTCAGGAAC TCACAAATGT CACCTTAACA ATTCAGAGTG TATGCCAATT
    AAAGGCCTAG GATTCGTGCT TGGAGCCTAC CAGTGCATTT GCAAAGCAGG ATTCTATCAT
    CCTCAAGACT TCTCAGTGAA CAGCTTTCAG AGAAGTGCAA AAGATGTGTC AGAAGAAGTC
    CATGTCTGCC TACCCTGCAG GGAGGGCTGC CCCTACTGTG CTGATGACAG CCCCTGCTTT
    GTCCAGGAGG ATAAGTATTT GCGACTTGCT ATCATCTCCT TCCAAGCCCT GTGTATGCTG
    CTTGACTTCG TTAGCATGCT GGTGGTCTAC CACTTTCGCA AAGCAAAGAG CATCAGGGCA
    TCAGGCCTTA TCCTGTTGGA AACAATTCTT TTTGGGTCTC TGCTTCTATA CTTTCCTGTT
    GTTATTTTGT ATTTTGAGCC AAGCACATTT CGCTGTATTC TCCTAAGATG GGTCCGTCTT
    CTCGGTTTTG CTACTGTTTA CGGAACTGTC ACTCTCAAGC TTCACAGGGT TTTGAAGGTG
    TTTCTTTCAC GAACAGCCCA GCGGATTCCA TATATGACTG GTGGACGGGT CATGAGGATG
    CTGGCAGTAA TGCTCCTGGT AGTATTTTGG TTTCTTGTTG GCTGGACTTC GTCTGTTTGC
    CAGAATTTGG AGAGGCAGAT TTCACTTATT GGCCAAGGGC AAACATCTGA TCACCTCATC
    TTCAATATGT GCCTCATTGA GCGCTGGGAT TACATGACAG CAGTTGCTGA ATTTTTATTC
    CTCTTGTGGG GTGTTTATCT CTGCTATGCA GTGCGGACCG TCCCATCAGC ATTTCATGAG
    CCACGATATA TGGCTGTTGC AGTTCACAAT GAGCTCATTA TCTCTGCTAT ATTCCATACA
    ATTAGATTCG TTCTTGCCTC AAGACTTCAG TCTGACTGGA TGCTAATGCT GTATTTTGCA
    CATACTCATT TGACTGTGAC AGTCACCATT GGGTTGCTTT TGATTCCAAA GTTTTCACAT
    TCAAGCAATA ACCCCCGAGA TGACATTGCT ACAGAAGCAT ATGAAGATGA GCTAGACCTG
    GGTAGGTCTG GGTCCTACCT GAACAGCAGC ATCAACTCTG CATGGAGTGA GCACAGCTTG
    GACCCAGAGG ACATTCGGGA TGAGCTAAAG AAACTCTATG CCCAGTTGGA AATATATAAA
    AGGAAGAAGA TGATTACAAA TAACCCTCAC CTCCAGAAAA AGCGGTGCTC CAAGAAGGGC
    TTAGGCCGTT CCATCATGAG GCGTATAACC GAGATCCCAG AGACGGTTAG CAGGCAGTGT
    TCCAAAGAGG ACAAGGAAGT CACAGACCAC AGCACAGCCA AAAGCACTGC CCTGGCCAGG
    AAGAACCCAC AAGAATCTTC AGGTAACACC GGGAAGCCCA AAGAAGAGTC CCTGAAAAAC
    CGAGTCTTCT CGCTAAAGAA ATCACACAGC ACTTATGATC ATGTGAGGGA GCACACGGAA
    GAGTCCAACA GTTTACCAGT GGAAAGCCAA GATGAGGAGG CTACAGAACA TTCCACACTA
    GAGTCTCTGT CAAGTAAAAA ACTAACACAA AAACTGAAAG AAAACAGTGA GGCCGAGTCC
    ACAGAGTCCG TGCCTTTGGT GTGTAAGTCA GCAAGTGCAC ACAATCTCAG CTCAGAGAAG
    AAGGCAGGCC ACCCCCGAAC ATCGATGTTG CAAAAGTCTC TCAGTGTCAT CGCAAGCGCC
    AAGGAGAAGA CTCTTGGATT AGCTGGGAAA ACTCAAACAT CAGGCATGGA AGAACGTGCT
    AAAGCCCAGA AACCAAAAGA TAAAGAAACA AATAGAAAAT ACTCAAATTC AGATAATGCA
    GAGACTAATG ATTCTGCCCC CCAAAACTCC AATCATTTGG AGGAGCCAAG AAAACCTCAG
    AAATCTGGGA TTATGAAGCA ACAACGGGTC AACCCGGCCA CTGCCAATTC TGACATGAGC
    CCAGGCACCC CCCAGACGAA GAACAACTTT GACATTGGGG AAGTGTGCCC TTGGGAGATT
    TATGACCTGG CCCCCGGTCC TGTGCCTTCA GAATCAAAAG TTCAAAAACA CGTATCTATT
    GCAGCTTCTG AAATGGAGAA AAACCCAACT TTTTCCTTAA AGGAGAAGTC TCATCAGAAG
    CCCAAGGCAG CTGAACTGTG TCAGCAAACC AATCAGAAGA GCATAGACAA GGCTGAGGTG
    TGCCCTTGGA AGAGCCAAGG CCAGCCTGGT TTTGAAGATG AGAAGCATCT GATTCCCAAG
    ACTCAAGTTC CCGCAGGGAG AGCCAGAGAG GAGAATGGCA ATCAGCATTA TGCAGCCAAT
    GTGTGTGCCG GGCAGTATGA AGAACTGCCC CCCAAAGCTA TAGCATCAAA AGTCGAGAAT
    GAAAGTCTCA ACCAAACAGG AGACCAGGAG AAAAAGACAT CCTCCCCTGA GGAAAATGTG
    CCTGGCTCCT ATAACTCAAG TAATAACTTT CGGCAGCCTT TGACATCACG AGCTGAGGTG
    TGTCCTTGGG AGTTTGAGAC CCCAAATCAA CCAAATGCTG AAAGAAGTGT AGCTTTACCT
    GCCTCTTCCA CTTTAAGTGC AAATAAAATA GCAGGGCCTC GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35130
    Clone ID Related Accession (Same CDS sequence) XM_014121676.2 , XM_014121676.1
    Accession Version XM_014121676.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable G-protein coupled receptor 158
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726048.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCCA TGGCTTATCC CTTACTCTTC TGCCTCCTGC TCGCTCATCT GGGTTTGGGA 
    ACTGTTAGCA CCAGCCGCGA CCCTCCGGGA CGGCTGGATT CCCCTCGAGA GAGAAGCCTG
    AGGCTGAAGC AGCACGCCCA GCAGCCCGCG CGAGCCGCTG CCTCGGACCC CTCGGCTCCC
    TGGAGCCGCT CCACCGACGG CACCATCTTG GCGCAGAAAC TCGCCGAGGA GGTGCCCATG
    GACGTGGCCT CTTACCTCTA CACCGGGGAC TCCCACCAGC TGAAGCGAGC CAACTGCTCC
    GGCCGCTACG AGTTGGCGGG CCTGCCGGGG AAGTCGTCGG CCCTGGCCAG CTCCCACCCC
    TCCTTGCACG GGGCGCTGGA CACGCTGACA CACGCCACTA ACTTCCTCAA CATGATGCTG
    CAGAGCAATA AGTCGCGGGA GCAGAACTTG CAGGACGACC TGGAGTGGTA CCAGGCACTG
    GTGCGGAGTC TGCTGGAGGG CGAGCCCAGC ATTTCCCGGG CAGCCATCAC CTTCAGCACC
    GAGTCGCTGT CCGCGCCCGC CCCGCAGGTT TTCCTCCAGG CCACCCGCGA GGAGAGTCGC
    ATCCTGCTCC AGGACCTGTC CTCCTCGGCA CACCACCTGG CCAACGCCAC GCTGGAGACC
    GAGTGGTTCC ACGGCCTGCG GCGCAAGTGG CGGACCCACT TACACCGCCG CGGCTCTAAC
    CAGGGGCCCC GGAGCCTGGG CCATAGCTGG CGGCGCCGGG ACGGGCTTGG CGGGGACAAG
    AGCCATGTCA AGTGGTCCCC GCCTTATTTG GAGTGCGAGA ACGGGAGTTA CAAGCCTGGG
    TGGCTGGTCA CTCTCTCCGC AGCTTTCTAC GGGTTGCAGC CTAACCTGGT CCCCGAATTC
    AGGGGTGTTA TGAAAGTTGA CATAAATCTT CAGAAAGTGG ACATTGACCA ATGTTCAAGT
    GATGGCTGGT TTTCAGGAAC TCACAAATGT CACCTTAACA ATTCAGAGTG TATGCCAATT
    AAAGGCCTAG GATTCGTGCT TGGAGCCTAC CAGTGCATTT GCAAAGCAGG ATTCTATCAT
    CCTCAAGACT TCTCAGTGAA CAGCTTTCAG AGAAGTGCAA AAGATGTGTC AGAAGAAGTC
    CATGTCTGCC TACCCTGCAG GGAGGGCTGC CCCTACTGTG CTGATGACAG CCCCTGCTTT
    GTCCAGGAGG ATAAGTATTT GCGACTTGCT ATCATCTCCT TCCAAGCCCT GTGTATGCTG
    CTTGACTTCG TTAGCATGCT GGTGGTCTAC CACTTTCGCA AAGCAAAGAG CATCAGGGCA
    TCAGGCCTTA TCCTGTTGGA AACAATTCTT TTTGGGTCTC TGCTTCTATA CTTTCCTGTT
    GTTATTTTGT ATTTTGAGCC AAGCACATTT CGCTGTATTC TCCTAAGATG GGTCCGTCTT
    CTCGGTTTTG CTACTGTTTA CGGAACTGTC ACTCTCAAGC TTCACAGGGT TTTGAAGGTG
    TTTCTTTCAC GAACAGCCCA GCGGATTCCA TATATGACTG GTGGACGGGT CATGAGGATG
    CTGGCAGTAA TGCTCCTGGT AGTATTTTGG TTTCTTGTTG GCTGGACTTC GTCTGTTTGC
    CAGAATTTGG AGAGGCAGAT TTCACTTATT GGCCAAGGGC AAACATCTGA TCACCTCATC
    TTCAATATGT GCCTCATTGA GCGCTGGGAT TACATGACAG CAGTTGCTGA ATTTTTATTC
    CTCTTGTGGG GTGTTTATCT CTGCTATGCA GTGCGGACCG TCCCATCAGC ATTTCATGAG
    CCACGATATA TGGCTGTTGC AGTTCACAAT GAGCTCATTA TCTCTGCTAT ATTCCATACA
    ATTAGATTCG TTCTTGCCTC AAGACTTCAG TCTGACTGGA TGCTAATGCT GTATTTTGCA
    CATACTCATT TGACTGTGAC AGTCACCATT GGGTTGCTTT TGATTCCAAA GTTTTCACAT
    TCAAGCAATA ACCCCCGAGA TGACATTGCT ACAGAAGCAT ATGAAGATGA GCTAGACCTG
    GGTAGGTCTG GGTCCTACCT GAACAGCAGC ATCAACTCTG CATGGAGTGA GCACAGCTTG
    GACCCAGAGG ACATTCGGGA TGAGCTAAAG AAACTCTATG CCCAGTTGGA AATATATAAA
    AGGAAGAAGA TGATTACAAA TAACCCTCAC CTCCAGAAAA AGCGGTGCTC CAAGAAGGGC
    TTAGGCCGTT CCATCATGAG GCGTATAACC GAGATCCCAG AGACGGTTAG CAGGCAGTGT
    TCCAAAGAGG ACAAGGAAGT CACAGACCAC AGCACAGCCA AAAGCACTGC CCTGGCCAGG
    AAGAACCCAC AAGAATCTTC AGGTAACACC GGGAAGCCCA AAGAAGAGTC CCTGAAAAAC
    CGAGTCTTCT CGCTAAAGAA ATCACACAGC ACTTATGATC ATGTGAGGGA GCACACGGAA
    GAGTCCAACA GTTTACCAGT GGAAAGCCAA GATGAGGAGG CTACAGAACA TTCCACACTA
    GAGTCTCTGT CAAGTAAAAA ACTAACACAA AAACTGAAAG AAAACAGTGA GGCCGAGTCC
    ACAGAGTCCG TGCCTTTGGT GTGTAAGTCA GCAAGTGCAC ACAATCTCAG CTCAGAGAAG
    AAGGCAGGCC ACCCCCGAAC ATCGATGTTG CAAAAGTCTC TCAGTGTCAT CGCAAGCGCC
    AAGGAGAAGA CTCTTGGATT AGCTGGGAAA ACTCAAACAT CAGGCATGGA AGAACGTGCT
    AAAGCCCAGA AACCAAAAGA TAAAGAAACA AATAGAAAAT ACTCAAATTC AGATAATGCA
    GAGACTAATG ATTCTGCCCC CCAAAACTCC AATCATTTGG AGGAGCCAAG AAAACCTCAG
    AAATCTGGGA TTATGAAGCA ACAACGGGTC AACCCGGCCA CTGCCAATTC TGACATGAGC
    CCAGGCACCC CCCAGACGAA GAACAACTTT GACATTGGGG AAGTGTGCCC TTGGGAGATT
    TATGACCTGG CCCCCGGTCC TGTGCCTTCA GAATCAAAAG TTCAAAAACA CGTATCTATT
    GCAGCTTCTG AAATGGAGAA AAACCCAACT TTTTCCTTAA AGGAGAAGTC TCATCAGAAG
    CCCAAGGCAG CTGAACTGTG TCAGCAAACC AATCAGAAGA GCATAGACAA GGCTGAGGTG
    TGCCCTTGGA AGAGCCAAGG CCAGCCTGGT TTTGAAGATG AGAAGCATCT GATTCCCAAG
    ACTCAAGTTC CCGCAGGGAG AGCCAGAGAG GAGAATGGCA ATCAGCATTA TGCAGCCAAT
    GTGTGTGCCG GGCAGTATGA AGAACTGCCC CCCAAAGCTA TAGCATCAAA AGTCGAGAAT
    GAAAGTCTCA ACCAAACAGG AGACCAGGAG AAAAAGACAT CCTCCCCTGA GGAAAATGTG
    CCTGGCTCCT ATAACTCAAG TAATAACTTT CGGCAGCCTT TGACATCACG AGCTGAGGTG
    TGTCCTTGGG AGTTTGAGAC CCCAAATCAA CCAAATGCTG AAAGAAGTGT AGCTTTACCT
    GCCTCTTCCA CTTTAAGTGC AAATAAAATA GCAGGGCCTC GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013977151.1
    CDS672..4259
    Translation

    Target ORF information:

    RefSeq Version XM_014121676.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor 158 (GPR158), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121676.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCCA TGGCTTATCC CTTACTCTTC TGCCTCCTGC TCGCTCATCT GGGTTTGGGA 
    ACTGTTAGCA CCAGCCGCGA CCCTCCGGGA CGGCTGGATT CCCCTCGAGA GAGAAGCCTG
    AGGCTGAAGC AGCACGCCCA GCAGCCCGCG CGAGCCGCTG CCTCGGACCC CTCGGCTCCC
    TGGAGCCGCT CCACCGACGG CACCATCTTG GCGCAGAAAC TCGCCGAGGA GGTGCCCATG
    GACGTGGCCT CTTACCTCTA CACCGGGGAC TCCCACCAGC TGAAGCGAGC CAACTGCTCC
    GGCCGCTACG AGTTGGCGGG CCTGCCGGGG AAGTCGTCGG CCCTGGCCAG CTCCCACCCC
    TCCTTGCACG GGGCGCTGGA CACGCTGACA CACGCCACTA ACTTCCTCAA CATGATGCTG
    CAGAGCAATA AGTCGCGGGA GCAGAACTTG CAGGACGACC TGGAGTGGTA CCAGGCACTG
    GTGCGGAGTC TGCTGGAGGG CGAGCCCAGC ATTTCCCGGG CAGCCATCAC CTTCAGCACC
    GAGTCGCTGT CCGCGCCCGC CCCGCAGGTT TTCCTCCAGG CCACCCGCGA GGAGAGTCGC
    ATCCTGCTCC AGGACCTGTC CTCCTCGGCA CACCACCTGG CCAACGCCAC GCTGGAGACC
    GAGTGGTTCC ACGGCCTGCG GCGCAAGTGG CGGACCCACT TACACCGCCG CGGCTCTAAC
    CAGGGGCCCC GGAGCCTGGG CCATAGCTGG CGGCGCCGGG ACGGGCTTGG CGGGGACAAG
    AGCCATGTCA AGTGGTCCCC GCCTTATTTG GAGTGCGAGA ACGGGAGTTA CAAGCCTGGG
    TGGCTGGTCA CTCTCTCCGC AGCTTTCTAC GGGTTGCAGC CTAACCTGGT CCCCGAATTC
    AGGGGTGTTA TGAAAGTTGA CATAAATCTT CAGAAAGTGG ACATTGACCA ATGTTCAAGT
    GATGGCTGGT TTTCAGGAAC TCACAAATGT CACCTTAACA ATTCAGAGTG TATGCCAATT
    AAAGGCCTAG GATTCGTGCT TGGAGCCTAC CAGTGCATTT GCAAAGCAGG ATTCTATCAT
    CCTCAAGACT TCTCAGTGAA CAGCTTTCAG AGAAGTGCAA AAGATGTGTC AGAAGAAGTC
    CATGTCTGCC TACCCTGCAG GGAGGGCTGC CCCTACTGTG CTGATGACAG CCCCTGCTTT
    GTCCAGGAGG ATAAGTATTT GCGACTTGCT ATCATCTCCT TCCAAGCCCT GTGTATGCTG
    CTTGACTTCG TTAGCATGCT GGTGGTCTAC CACTTTCGCA AAGCAAAGAG CATCAGGGCA
    TCAGGCCTTA TCCTGTTGGA AACAATTCTT TTTGGGTCTC TGCTTCTATA CTTTCCTGTT
    GTTATTTTGT ATTTTGAGCC AAGCACATTT CGCTGTATTC TCCTAAGATG GGTCCGTCTT
    CTCGGTTTTG CTACTGTTTA CGGAACTGTC ACTCTCAAGC TTCACAGGGT TTTGAAGGTG
    TTTCTTTCAC GAACAGCCCA GCGGATTCCA TATATGACTG GTGGACGGGT CATGAGGATG
    CTGGCAGTAA TGCTCCTGGT AGTATTTTGG TTTCTTGTTG GCTGGACTTC GTCTGTTTGC
    CAGAATTTGG AGAGGCAGAT TTCACTTATT GGCCAAGGGC AAACATCTGA TCACCTCATC
    TTCAATATGT GCCTCATTGA GCGCTGGGAT TACATGACAG CAGTTGCTGA ATTTTTATTC
    CTCTTGTGGG GTGTTTATCT CTGCTATGCA GTGCGGACCG TCCCATCAGC ATTTCATGAG
    CCACGATATA TGGCTGTTGC AGTTCACAAT GAGCTCATTA TCTCTGCTAT ATTCCATACA
    ATTAGATTCG TTCTTGCCTC AAGACTTCAG TCTGACTGGA TGCTAATGCT GTATTTTGCA
    CATACTCATT TGACTGTGAC AGTCACCATT GGGTTGCTTT TGATTCCAAA GTTTTCACAT
    TCAAGCAATA ACCCCCGAGA TGACATTGCT ACAGAAGCAT ATGAAGATGA GCTAGACCTG
    GGTAGGTCTG GGTCCTACCT GAACAGCAGC ATCAACTCTG CATGGAGTGA GCACAGCTTG
    GACCCAGAGG ACATTCGGGA TGAGCTAAAG AAACTCTATG CCCAGTTGGA AATATATAAA
    AGGAAGAAGA TGATTACAAA TAACCCTCAC CTCCAGAAAA AGCGGTGCTC CAAGAAGGGC
    TTAGGCCGTT CCATCATGAG GCGTATAACC GAGATCCCAG AGACGGTTAG CAGGCAGTGT
    TCCAAAGAGG ACAAGGAAGT CACAGACCAC AGCACAGCCA AAAGCACTGC CCTGGCCAGG
    AAGAACCCAC AAGAATCTTC AGGTAACACC GGGAAGCCCA AAGAAGAGTC CCTGAAAAAC
    CGAGTCTTCT CGCTAAAGAA ATCACACAGC ACTTATGATC ATGTGAGGGA GCACACGGAA
    GAGTCCAACA GTTTACCAGT GGAAAGCCAA GATGAGGAGG CTACAGAACA TTCCACACTA
    GAGTCTCTGT CAAGTAAAAA ACTAACACAA AAACTGAAAG AAAACAGTGA GGCCGAGTCC
    ACAGAGTCCG TGCCTTTGGT GTGTAAGTCA GCAAGTGCAC ACAATCTCAG CTCAGAGAAG
    AAGGCAGGCC ACCCCCGAAC ATCGATGTTG CAAAAGTCTC TCAGTGTCAT CGCAAGCGCC
    AAGGAGAAGA CTCTTGGATT AGCTGGGAAA ACTCAAACAT CAGGCATGGA AGAACGTGCT
    AAAGCCCAGA AACCAAAAGA TAAAGAAACA AATAGAAAAT ACTCAAATTC AGATAATGCA
    GAGACTAATG ATTCTGCCCC CCAAAACTCC AATCATTTGG AGGAGCCAAG AAAACCTCAG
    AAATCTGGGA TTATGAAGCA ACAACGGGTC AACCCGGCCA CTGCCAATTC TGACATGAGC
    CCAGGCACCC CCCAGACGAA GAACAACTTT GACATTGGGG AAGTGTGCCC TTGGGAGATT
    TATGACCTGG CCCCCGGTCC TGTGCCTTCA GAATCAAAAG TTCAAAAACA CGTATCTATT
    GCAGCTTCTG AAATGGAGAA AAACCCAACT TTTTCCTTAA AGGAGAAGTC TCATCAGAAG
    CCCAAGGCAG CTGAACTGTG TCAGCAAACC AATCAGAAGA GCATAGACAA GGCTGAGGTG
    TGCCCTTGGA AGAGCCAAGG CCAGCCTGGT TTTGAAGATG AGAAGCATCT GATTCCCAAG
    ACTCAAGTTC CCGCAGGGAG AGCCAGAGAG GAGAATGGCA ATCAGCATTA TGCAGCCAAT
    GTGTGTGCCG GGCAGTATGA AGAACTGCCC CCCAAAGCTA TAGCATCAAA AGTCGAGAAT
    GAAAGTCTCA ACCAAACAGG AGACCAGGAG AAAAAGACAT CCTCCCCTGA GGAAAATGTG
    CCTGGCTCCT ATAACTCAAG TAATAACTTT CGGCAGCCTT TGACATCACG AGCTGAGGTG
    TGTCCTTGGG AGTTTGAGAC CCCAAATCAA CCAAATGCTG AAAGAAGTGT AGCTTTACCT
    GCCTCTTCCA CTTTAAGTGC AAATAAAATA GCAGGGCCTC GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.