DDX27 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX27
Entrez Gene ID	477259
Full Name	DEAD-box helicase 27
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014107010.1	XP_013962485.1	probable ATP-dependent RNA helicase DDX27 isoform X1
XM_014107010.2	XP_013962485.1	probable ATP-dependent RNA helicase DDX27 isoform X1
XM_534451.6	XP_534451.2	probable ATP-dependent RNA helicase DDX27 isoform X2
XM_534451.5	XP_534451.2	probable ATP-dependent RNA helicase DDX27 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX27	NP_060365.7
Pan troglodytes (chimpanzee)	DDX27	XP_001166506.1
Danio rerio (zebrafish)	ddx27	NP_001002869.1
	DDX27	XP_001099400.1
Canis lupus familiaris (dog)	DDX27	XP_534451.2
Mus musculus (house mouse)	Ddx27	NP_694705.2
Gallus gallus (chicken)	DDX27	NP_001006293.1
Rattus norvegicus (Norway rat)	Ddx27	NP_001129273.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011084	XP_309566.4
Xenopus tropicalis (tropical clawed frog)	ddx27	XP_004919149.1
Bos taurus (cattle)	DDX27	NP_001073740.1
Drosophila melanogaster (fruit fly)	Rs1	NP_651970.1
Caenorhabditis elegans (roundworm)	Y71G12B.8	NP_490891.2

Gene Ontology

The following DDX27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe25034	XM_534451.6 Latest version!	Canis lupus familiaris DEAD-box helicase 27 (DDX27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe42739	XM_014107010.2 Latest version!	Canis lupus familiaris DEAD-box helicase 27 (DDX27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe42739	XM_014107010.1	Canis lupus familiaris DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe25034	XM_534451.5	Canis lupus familiaris DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX27 ( XM_534451.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_534451.2 Canis lupus familiaris DEAD-box helicase 27 (DDX27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe25034
Clone ID Related Accession (Same CDS sequence)	XM_534451.6 , XM_534451.5
Accession Version	XM_534451.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable ATP-dependent RNA helicase DDX27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534451.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGCTC TTCTGACAAG AACCTTCACT GACCATGTGA TGCTGTTCAC CCAGACCAAG 
AAGCAGGCCC ACCGCATGCA CATCCTTCTG GGGCTCATGG GGCTGCAGGT TGGCGAGCTC 
CATGGCAACC TGTCCCAGAC GCAGCGGCTA GAGGCCCTCC GGCGCTTTAA GGACGAACAG 
ATTGACATCC TTGTGGCCAC AGATGTGGCA GCCCGTGGAC TTGACATTGA AGGGGTCAAA 
ACGGTAATTA ACTTCACGAT GCCCAACACC ATCAAGCATT ATGTCCACCG GGTGGGGCGA 
ACAGCTCGGG CTGGTAGGGC TGGGCGCTCG GTGTCTCTGG TGGGAGAAGA GGAGCGGAAG 
ATGCTGAAGG AGATCGTAAA AGCCGCCAAA GCCCCTGTTA AGGCCCGGAT ACTTCCCCAG 
GATGTCATCC TTAAATTCCG GGACAAGATT GAGAAGATGG AGAAGGATGT GTATGCAGTT 
CTGCAGCTAG AGGCCGAGGA AAAAGAGCTG CAGCAGTCAG AAGCCCAGAT CAACACAGCA 
AAACGGCTCC TTGAGAGAGA GAAGGGGAAG GAGGCCTCAA ACCATGAGCC TGAGAGAAGC 
TGGTTCCAGA CCAAGGAAGA GAGGAAGAAG GAAAAAATTG CCAAAGCTCT GCAGGAGTTT 
GACTTGGCCT TAAGAGGAAA GAAGAAGAGA AAGAAGTTTA TGAAGGATGC CAAGAAAAAG 
GGGGAGATGA CAGCAGAGGA AAGGTCCCAG TTTGAAATCC TCAAGGCACA GATGTTTGCT 
GAGCGGCTGG CCAAGAGGAA TCGCAGAGCC AAGAGGGCCC GAGCAATGCC TGAGGAGGAG 
CCAGCAAGAG GCCCCGCCAA GAAACAAAAG CAGGTGAATA AATCTGTATT TGATGAAGAA 
CTTACCAACA CAAGCAGGAA GGCCCTGAAA CAGTATCGAG CTGGCCCCTC TTTTGAAGAA 
AGGAAACAGT TGGGCCTGCC CCACCAGAGA CGAGGAGGAA ACTTTAAGTC TAAATCCAGG 
TACAAGAGAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534451.2
CDS	77..2374
Translation	MLEELGLIGTIGEDDDVPGHPESDSGDEEEEGPVVLGRKQKALQKNRSADFNPDFVFTEKEGAYDGSWAMADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKSEEKEAKEGSELEEQEDHEGKDEEGPEDEDSETDYSSADENILTKADTLKVKERKKKKKGQETGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQSPVTRVLVLVPTRELGIQVHSVTKQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFVRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKELQQSEAQINTAKRLLEREKGKEASNHEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPARGPAKKQKQVNKSVFDEELTNTSRKALKQYRAGPSFEERKQLGLPHQRRGGNFKSKSRYKRKK

Target ORF information:

RefSeq Version	XM_534451.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DEAD-box helicase 27 (DDX27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534451.6

ORF Insert Sequence:

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGCTC TTCTGACAAG AACCTTCACT GACCATGTGA TGCTGTTCAC CCAGACCAAG 
AAGCAGGCCC ACCGCATGCA CATCCTTCTG GGGCTCATGG GGCTGCAGGT TGGCGAGCTC 
CATGGCAACC TGTCCCAGAC GCAGCGGCTA GAGGCCCTCC GGCGCTTTAA GGACGAACAG 
ATTGACATCC TTGTGGCCAC AGATGTGGCA GCCCGTGGAC TTGACATTGA AGGGGTCAAA 
ACGGTAATTA ACTTCACGAT GCCCAACACC ATCAAGCATT ATGTCCACCG GGTGGGGCGA 
ACAGCTCGGG CTGGTAGGGC TGGGCGCTCG GTGTCTCTGG TGGGAGAAGA GGAGCGGAAG 
ATGCTGAAGG AGATCGTAAA AGCCGCCAAA GCCCCTGTTA AGGCCCGGAT ACTTCCCCAG 
GATGTCATCC TTAAATTCCG GGACAAGATT GAGAAGATGG AGAAGGATGT GTATGCAGTT 
CTGCAGCTAG AGGCCGAGGA AAAAGAGCTG CAGCAGTCAG AAGCCCAGAT CAACACAGCA 
AAACGGCTCC TTGAGAGAGA GAAGGGGAAG GAGGCCTCAA ACCATGAGCC TGAGAGAAGC 
TGGTTCCAGA CCAAGGAAGA GAGGAAGAAG GAAAAAATTG CCAAAGCTCT GCAGGAGTTT 
GACTTGGCCT TAAGAGGAAA GAAGAAGAGA AAGAAGTTTA TGAAGGATGC CAAGAAAAAG 
GGGGAGATGA CAGCAGAGGA AAGGTCCCAG TTTGAAATCC TCAAGGCACA GATGTTTGCT 
GAGCGGCTGG CCAAGAGGAA TCGCAGAGCC AAGAGGGCCC GAGCAATGCC TGAGGAGGAG 
CCAGCAAGAG GCCCCGCCAA GAAACAAAAG CAGGTGAATA AATCTGTATT TGATGAAGAA 
CTTACCAACA CAAGCAGGAA GGCCCTGAAA CAGTATCGAG CTGGCCCCTC TTTTGAAGAA 
AGGAAACAGT TGGGCCTGCC CCACCAGAGA CGAGGAGGAA ACTTTAAGTC TAAATCCAGG 
TACAAGAGAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX27 ( XM_534451.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_534451.2 Canis lupus familiaris DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe25034
Clone ID Related Accession (Same CDS sequence)	XM_534451.6 , XM_534451.5
Accession Version	XM_534451.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable ATP-dependent RNA helicase DDX27 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534451.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGCTC TTCTGACAAG AACCTTCACT GACCATGTGA TGCTGTTCAC CCAGACCAAG 
AAGCAGGCCC ACCGCATGCA CATCCTTCTG GGGCTCATGG GGCTGCAGGT TGGCGAGCTC 
CATGGCAACC TGTCCCAGAC GCAGCGGCTA GAGGCCCTCC GGCGCTTTAA GGACGAACAG 
ATTGACATCC TTGTGGCCAC AGATGTGGCA GCCCGTGGAC TTGACATTGA AGGGGTCAAA 
ACGGTAATTA ACTTCACGAT GCCCAACACC ATCAAGCATT ATGTCCACCG GGTGGGGCGA 
ACAGCTCGGG CTGGTAGGGC TGGGCGCTCG GTGTCTCTGG TGGGAGAAGA GGAGCGGAAG 
ATGCTGAAGG AGATCGTAAA AGCCGCCAAA GCCCCTGTTA AGGCCCGGAT ACTTCCCCAG 
GATGTCATCC TTAAATTCCG GGACAAGATT GAGAAGATGG AGAAGGATGT GTATGCAGTT 
CTGCAGCTAG AGGCCGAGGA AAAAGAGCTG CAGCAGTCAG AAGCCCAGAT CAACACAGCA 
AAACGGCTCC TTGAGAGAGA GAAGGGGAAG GAGGCCTCAA ACCATGAGCC TGAGAGAAGC 
TGGTTCCAGA CCAAGGAAGA GAGGAAGAAG GAAAAAATTG CCAAAGCTCT GCAGGAGTTT 
GACTTGGCCT TAAGAGGAAA GAAGAAGAGA AAGAAGTTTA TGAAGGATGC CAAGAAAAAG 
GGGGAGATGA CAGCAGAGGA AAGGTCCCAG TTTGAAATCC TCAAGGCACA GATGTTTGCT 
GAGCGGCTGG CCAAGAGGAA TCGCAGAGCC AAGAGGGCCC GAGCAATGCC TGAGGAGGAG 
CCAGCAAGAG GCCCCGCCAA GAAACAAAAG CAGGTGAATA AATCTGTATT TGATGAAGAA 
CTTACCAACA CAAGCAGGAA GGCCCTGAAA CAGTATCGAG CTGGCCCCTC TTTTGAAGAA 
AGGAAACAGT TGGGCCTGCC CCACCAGAGA CGAGGAGGAA ACTTTAAGTC TAAATCCAGG 
TACAAGAGAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534451.2
CDS	43..2340
Translation	MLEELGLIGTIGEDDDVPGHPESDSGDEEEEGPVVLGRKQKALQKNRSADFNPDFVFTEKEGAYDGSWAMADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKSEEKEAKEGSELEEQEDHEGKDEEGPEDEDSETDYSSADENILTKADTLKVKERKKKKKGQETGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQSPVTRVLVLVPTRELGIQVHSVTKQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFVRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKELQQSEAQINTAKRLLEREKGKEASNHEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPARGPAKKQKQVNKSVFDEELTNTSRKALKQYRAGPSFEERKQLGLPHQRRGGNFKSKSRYKRKK

Target ORF information:

RefSeq Version	XM_534451.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534451.5

ORF Insert Sequence:

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGCTC TTCTGACAAG AACCTTCACT GACCATGTGA TGCTGTTCAC CCAGACCAAG 
AAGCAGGCCC ACCGCATGCA CATCCTTCTG GGGCTCATGG GGCTGCAGGT TGGCGAGCTC 
CATGGCAACC TGTCCCAGAC GCAGCGGCTA GAGGCCCTCC GGCGCTTTAA GGACGAACAG 
ATTGACATCC TTGTGGCCAC AGATGTGGCA GCCCGTGGAC TTGACATTGA AGGGGTCAAA 
ACGGTAATTA ACTTCACGAT GCCCAACACC ATCAAGCATT ATGTCCACCG GGTGGGGCGA 
ACAGCTCGGG CTGGTAGGGC TGGGCGCTCG GTGTCTCTGG TGGGAGAAGA GGAGCGGAAG 
ATGCTGAAGG AGATCGTAAA AGCCGCCAAA GCCCCTGTTA AGGCCCGGAT ACTTCCCCAG 
GATGTCATCC TTAAATTCCG GGACAAGATT GAGAAGATGG AGAAGGATGT GTATGCAGTT 
CTGCAGCTAG AGGCCGAGGA AAAAGAGCTG CAGCAGTCAG AAGCCCAGAT CAACACAGCA 
AAACGGCTCC TTGAGAGAGA GAAGGGGAAG GAGGCCTCAA ACCATGAGCC TGAGAGAAGC 
TGGTTCCAGA CCAAGGAAGA GAGGAAGAAG GAAAAAATTG CCAAAGCTCT GCAGGAGTTT 
GACTTGGCCT TAAGAGGAAA GAAGAAGAGA AAGAAGTTTA TGAAGGATGC CAAGAAAAAG 
GGGGAGATGA CAGCAGAGGA AAGGTCCCAG TTTGAAATCC TCAAGGCACA GATGTTTGCT 
GAGCGGCTGG CCAAGAGGAA TCGCAGAGCC AAGAGGGCCC GAGCAATGCC TGAGGAGGAG 
CCAGCAAGAG GCCCCGCCAA GAAACAAAAG CAGGTGAATA AATCTGTATT TGATGAAGAA 
CTTACCAACA CAAGCAGGAA GGCCCTGAAA CAGTATCGAG CTGGCCCCTC TTTTGAAGAA 
AGGAAACAGT TGGGCCTGCC CCACCAGAGA CGAGGAGGAA ACTTTAAGTC TAAATCCAGG 
TACAAGAGAA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX27 ( XM_014107010.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962485.1 Canis lupus familiaris DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), transcript variant X1, mRNA.

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CloneID	OCe42739
Clone ID Related Accession (Same CDS sequence)	XM_014107010.1 , XM_014107010.2
Accession Version	XM_014107010.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable ATP-dependent RNA helicase DDX27 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGACC ACAGTCCACA CTCCCCAGTG AGTCCTGTAC AGCCCTCTGG GACCTGGCCC 
CTGCCTGCCT GGAGTCTTCA GCTTTGGTGC CCTGCCATAC TCTCCTGTTT ATATTGTCTC 
AGACCTACCA AGCTTGTCCT GCCTCGGGGC CTTCACATTT GCTCCTTCCT CTGCCTGAAA 
TCTTCCCCCA AATCTTCTCT TCTGACAAGA ACCTTCACTG ACCATGTGAT GCTGTTCACC 
CAGACCAAGA AGCAGGCCCA CCGCATGCAC ATCCTTCTGG GGCTCATGGG GCTGCAGGTT 
GGCGAGCTCC ATGGCAACCT GTCCCAGACG CAGCGGCTAG AGGCCCTCCG GCGCTTTAAG 
GACGAACAGA TTGACATCCT TGTGGCCACA GATGTGGCAG CCCGTGGACT TGACATTGAA 
GGGGTCAAAA CGGTAATTAA CTTCACGATG CCCAACACCA TCAAGCATTA TGTCCACCGG 
GTGGGGCGAA CAGCTCGGGC TGGTAGGGCT GGGCGCTCGG TGTCTCTGGT GGGAGAAGAG 
GAGCGGAAGA TGCTGAAGGA GATCGTAAAA GCCGCCAAAG CCCCTGTTAA GGCCCGGATA 
CTTCCCCAGG ATGTCATCCT TAAATTCCGG GACAAGATTG AGAAGATGGA GAAGGATGTG 
TATGCAGTTC TGCAGCTAGA GGCCGAGGAA AAAGAGCTGC AGCAGTCAGA AGCCCAGATC 
AACACAGCAA AACGGCTCCT TGAGAGAGAG AAGGGGAAGG AGGCCTCAAA CCATGAGCCT 
GAGAGAAGCT GGTTCCAGAC CAAGGAAGAG AGGAAGAAGG AAAAAATTGC CAAAGCTCTG 
CAGGAGTTTG ACTTGGCCTT AAGAGGAAAG AAGAAGAGAA AGAAGTTTAT GAAGGATGCC 
AAGAAAAAGG GGGAGATGAC AGCAGAGGAA AGGTCCCAGT TTGAAATCCT CAAGGCACAG 
ATGTTTGCTG AGCGGCTGGC CAAGAGGAAT CGCAGAGCCA AGAGGGCCCG AGCAATGCCT 
GAGGAGGAGC CAGCAAGAGG CCCCGCCAAG AAACAAAAGC AGGTGAATAA ATCTGTATTT 
GATGAAGAAC TTACCAACAC AAGCAGGAAG GCCCTGAAAC AGTATCGAGC TGGCCCCTCT 
TTTGAAGAAA GGAAACAGTT GGGCCTGCCC CACCAGAGAC GAGGAGGAAA CTTTAAGTCT 
AAATCCAGGT ACAAGAGAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962485.1
CDS	76..2562
Translation	MLEELGLIGTIGEDDDVPGHPESDSGDEEEEGPVVLGRKQKALQKNRSADFNPDFVFTEKEGAYDGSWAMADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKSEEKEAKEGSELEEQEDHEGKDEEGPEDEDSETDYSSADENILTKADTLKVKERKKKKKGQETGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQSPVTRVLVLVPTRELGIQVHSVTKQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFVRIRPNREGDREAIVADHSPHSPVSPVQPSGTWPLPAWSLQLWCPAILSCLYCLRPTKLVLPRGLHICSFLCLKSSPKSSLLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKELQQSEAQINTAKRLLEREKGKEASNHEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPARGPAKKQKQVNKSVFDEELTNTSRKALKQYRAGPSFEERKQLGLPHQRRGGNFKSKSRYKRKK

Target ORF information:

RefSeq Version	XM_014107010.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107010.1

ORF Insert Sequence:

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGACC ACAGTCCACA CTCCCCAGTG AGTCCTGTAC AGCCCTCTGG GACCTGGCCC 
CTGCCTGCCT GGAGTCTTCA GCTTTGGTGC CCTGCCATAC TCTCCTGTTT ATATTGTCTC 
AGACCTACCA AGCTTGTCCT GCCTCGGGGC CTTCACATTT GCTCCTTCCT CTGCCTGAAA 
TCTTCCCCCA AATCTTCTCT TCTGACAAGA ACCTTCACTG ACCATGTGAT GCTGTTCACC 
CAGACCAAGA AGCAGGCCCA CCGCATGCAC ATCCTTCTGG GGCTCATGGG GCTGCAGGTT 
GGCGAGCTCC ATGGCAACCT GTCCCAGACG CAGCGGCTAG AGGCCCTCCG GCGCTTTAAG 
GACGAACAGA TTGACATCCT TGTGGCCACA GATGTGGCAG CCCGTGGACT TGACATTGAA 
GGGGTCAAAA CGGTAATTAA CTTCACGATG CCCAACACCA TCAAGCATTA TGTCCACCGG 
GTGGGGCGAA CAGCTCGGGC TGGTAGGGCT GGGCGCTCGG TGTCTCTGGT GGGAGAAGAG 
GAGCGGAAGA TGCTGAAGGA GATCGTAAAA GCCGCCAAAG CCCCTGTTAA GGCCCGGATA 
CTTCCCCAGG ATGTCATCCT TAAATTCCGG GACAAGATTG AGAAGATGGA GAAGGATGTG 
TATGCAGTTC TGCAGCTAGA GGCCGAGGAA AAAGAGCTGC AGCAGTCAGA AGCCCAGATC 
AACACAGCAA AACGGCTCCT TGAGAGAGAG AAGGGGAAGG AGGCCTCAAA CCATGAGCCT 
GAGAGAAGCT GGTTCCAGAC CAAGGAAGAG AGGAAGAAGG AAAAAATTGC CAAAGCTCTG 
CAGGAGTTTG ACTTGGCCTT AAGAGGAAAG AAGAAGAGAA AGAAGTTTAT GAAGGATGCC 
AAGAAAAAGG GGGAGATGAC AGCAGAGGAA AGGTCCCAGT TTGAAATCCT CAAGGCACAG 
ATGTTTGCTG AGCGGCTGGC CAAGAGGAAT CGCAGAGCCA AGAGGGCCCG AGCAATGCCT 
GAGGAGGAGC CAGCAAGAGG CCCCGCCAAG AAACAAAAGC AGGTGAATAA ATCTGTATTT 
GATGAAGAAC TTACCAACAC AAGCAGGAAG GCCCTGAAAC AGTATCGAGC TGGCCCCTCT 
TTTGAAGAAA GGAAACAGTT GGGCCTGCCC CACCAGAGAC GAGGAGGAAA CTTTAAGTCT 
AAATCCAGGT ACAAGAGAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX27 ( XM_014107010.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962485.1 Canis lupus familiaris DEAD-box helicase 27 (DDX27), transcript variant X1, mRNA.

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CloneID	OCe42739
Clone ID Related Accession (Same CDS sequence)	XM_014107010.1 , XM_014107010.2
Accession Version	XM_014107010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable ATP-dependent RNA helicase DDX27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGACC ACAGTCCACA CTCCCCAGTG AGTCCTGTAC AGCCCTCTGG GACCTGGCCC 
CTGCCTGCCT GGAGTCTTCA GCTTTGGTGC CCTGCCATAC TCTCCTGTTT ATATTGTCTC 
AGACCTACCA AGCTTGTCCT GCCTCGGGGC CTTCACATTT GCTCCTTCCT CTGCCTGAAA 
TCTTCCCCCA AATCTTCTCT TCTGACAAGA ACCTTCACTG ACCATGTGAT GCTGTTCACC 
CAGACCAAGA AGCAGGCCCA CCGCATGCAC ATCCTTCTGG GGCTCATGGG GCTGCAGGTT 
GGCGAGCTCC ATGGCAACCT GTCCCAGACG CAGCGGCTAG AGGCCCTCCG GCGCTTTAAG 
GACGAACAGA TTGACATCCT TGTGGCCACA GATGTGGCAG CCCGTGGACT TGACATTGAA 
GGGGTCAAAA CGGTAATTAA CTTCACGATG CCCAACACCA TCAAGCATTA TGTCCACCGG 
GTGGGGCGAA CAGCTCGGGC TGGTAGGGCT GGGCGCTCGG TGTCTCTGGT GGGAGAAGAG 
GAGCGGAAGA TGCTGAAGGA GATCGTAAAA GCCGCCAAAG CCCCTGTTAA GGCCCGGATA 
CTTCCCCAGG ATGTCATCCT TAAATTCCGG GACAAGATTG AGAAGATGGA GAAGGATGTG 
TATGCAGTTC TGCAGCTAGA GGCCGAGGAA AAAGAGCTGC AGCAGTCAGA AGCCCAGATC 
AACACAGCAA AACGGCTCCT TGAGAGAGAG AAGGGGAAGG AGGCCTCAAA CCATGAGCCT 
GAGAGAAGCT GGTTCCAGAC CAAGGAAGAG AGGAAGAAGG AAAAAATTGC CAAAGCTCTG 
CAGGAGTTTG ACTTGGCCTT AAGAGGAAAG AAGAAGAGAA AGAAGTTTAT GAAGGATGCC 
AAGAAAAAGG GGGAGATGAC AGCAGAGGAA AGGTCCCAGT TTGAAATCCT CAAGGCACAG 
ATGTTTGCTG AGCGGCTGGC CAAGAGGAAT CGCAGAGCCA AGAGGGCCCG AGCAATGCCT 
GAGGAGGAGC CAGCAAGAGG CCCCGCCAAG AAACAAAAGC AGGTGAATAA ATCTGTATTT 
GATGAAGAAC TTACCAACAC AAGCAGGAAG GCCCTGAAAC AGTATCGAGC TGGCCCCTCT 
TTTGAAGAAA GGAAACAGTT GGGCCTGCCC CACCAGAGAC GAGGAGGAAA CTTTAAGTCT 
AAATCCAGGT ACAAGAGAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962485.1
CDS	79..2565
Translation	MLEELGLIGTIGEDDDVPGHPESDSGDEEEEGPVVLGRKQKALQKNRSADFNPDFVFTEKEGAYDGSWAMADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKSEEKEAKEGSELEEQEDHEGKDEEGPEDEDSETDYSSADENILTKADTLKVKERKKKKKGQETGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQSPVTRVLVLVPTRELGIQVHSVTKQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFVRIRPNREGDREAIVADHSPHSPVSPVQPSGTWPLPAWSLQLWCPAILSCLYCLRPTKLVLPRGLHICSFLCLKSSPKSSLLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKELQQSEAQINTAKRLLEREKGKEASNHEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPARGPAKKQKQVNKSVFDEELTNTSRKALKQYRAGPSFEERKQLGLPHQRRGGNFKSKSRYKRKK

Target ORF information:

RefSeq Version	XM_014107010.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DEAD-box helicase 27 (DDX27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107010.2

ORF Insert Sequence:

ATGCTCGAGG AGCTGGGGTT GATCGGGACC ATAGGCGAAG ATGACGACGT GCCGGGGCAT 
CCTGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCG TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCCGAT TTCAACCCTG ACTTCGTTTT CACTGAGAAG 
GAGGGGGCAT ATGATGGCAG CTGGGCCATG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
AGGGCAGCCA CCACATTAGA TGAGAAGATT GAGAAAGTTC GCAAGAAAAG GAAAACAGAG 
GATAAAGAAG CCAAGTCTGG GAAGTCCGAG GAGAAAGAAG CAAAGGAAGG CTCTGAACTG 
GAGGAGCAGG AAGACCATGA AGGGAAAGAT GAGGAAGGTC CAGAAGATGA AGACTCAGAG 
ACTGATTACT CGTCAGCTGA TGAGAACATC CTCACCAAAG CAGATACACT CAAAGTAAAG 
GAGCGGAAGA AGAAGAAGAA AGGACAGGAA ACAGGAGGAT TTTTTGAAGA TGCATCTCAG 
TATGATGAAA ACCTCTCATT CCAGGACATG AACCTTTCCC GCCCTCTTCT GAAGGCCATT 
ACAGCCATGG GCTTCAAACA GCCCACCCCA ATCCAGAAGG CATGCATACC TGTGGGTCTG 
TTGGGGAAGG ATATCTGTGC CTGTGCAGCC ACTGGGACAG GTAAAACTGC TGCTTTTGCC 
CTGCCTGTCC TGGAGCGTCT GATCTACAAA CCCCGCCAGT CTCCTGTCAC CCGTGTGCTC 
GTGCTGGTTC CCACCCGAGA ACTGGGCATC CAGGTGCACT CTGTCACCAA GCAGCTGGCT 
CAGTTCTGCA ACATCACCAC CTGCCTGGCC GTGGGAGGCC TGGATGTGAA GTCTCAGGAA 
GCAGCTCTTA GAGCAGCGCC TGACATCCTC ATTGCCACCC CAGGCCGGCT CATCGATCAT 
CTCCACAACT GTCCTTCCTT CCACCTGAGC AGTATTGAGG TGCTCATCCT GGATGAGGCT 
GACAGGATGC TGGATGAGTA CTTTGAAGAA CAGATGAAGG AGATCATCCG GATGTGTTCC 
CACCACCGCC AGACCATGCT CTTCTCAGCC ACCATGACGG ATGAGGTAAA AGATCTGGCT 
TCTGTTTCCT TGAAGAATCC TGTCCGAATA TTTGTGAATA GCAACACAGA TGTGGCCCCG 
TTCCTGCGGC AGGAGTTTGT CCGGATCCGG CCGAATCGGG AAGGGGACCG GGAAGCCATT 
GTGGCAGACC ACAGTCCACA CTCCCCAGTG AGTCCTGTAC AGCCCTCTGG GACCTGGCCC 
CTGCCTGCCT GGAGTCTTCA GCTTTGGTGC CCTGCCATAC TCTCCTGTTT ATATTGTCTC 
AGACCTACCA AGCTTGTCCT GCCTCGGGGC CTTCACATTT GCTCCTTCCT CTGCCTGAAA 
TCTTCCCCCA AATCTTCTCT TCTGACAAGA ACCTTCACTG ACCATGTGAT GCTGTTCACC 
CAGACCAAGA AGCAGGCCCA CCGCATGCAC ATCCTTCTGG GGCTCATGGG GCTGCAGGTT 
GGCGAGCTCC ATGGCAACCT GTCCCAGACG CAGCGGCTAG AGGCCCTCCG GCGCTTTAAG 
GACGAACAGA TTGACATCCT TGTGGCCACA GATGTGGCAG CCCGTGGACT TGACATTGAA 
GGGGTCAAAA CGGTAATTAA CTTCACGATG CCCAACACCA TCAAGCATTA TGTCCACCGG 
GTGGGGCGAA CAGCTCGGGC TGGTAGGGCT GGGCGCTCGG TGTCTCTGGT GGGAGAAGAG 
GAGCGGAAGA TGCTGAAGGA GATCGTAAAA GCCGCCAAAG CCCCTGTTAA GGCCCGGATA 
CTTCCCCAGG ATGTCATCCT TAAATTCCGG GACAAGATTG AGAAGATGGA GAAGGATGTG 
TATGCAGTTC TGCAGCTAGA GGCCGAGGAA AAAGAGCTGC AGCAGTCAGA AGCCCAGATC 
AACACAGCAA AACGGCTCCT TGAGAGAGAG AAGGGGAAGG AGGCCTCAAA CCATGAGCCT 
GAGAGAAGCT GGTTCCAGAC CAAGGAAGAG AGGAAGAAGG AAAAAATTGC CAAAGCTCTG 
CAGGAGTTTG ACTTGGCCTT AAGAGGAAAG AAGAAGAGAA AGAAGTTTAT GAAGGATGCC 
AAGAAAAAGG GGGAGATGAC AGCAGAGGAA AGGTCCCAGT TTGAAATCCT CAAGGCACAG 
ATGTTTGCTG AGCGGCTGGC CAAGAGGAAT CGCAGAGCCA AGAGGGCCCG AGCAATGCCT 
GAGGAGGAGC CAGCAAGAGG CCCCGCCAAG AAACAAAAGC AGGTGAATAA ATCTGTATTT 
GATGAAGAAC TTACCAACAC AAGCAGGAAG GCCCTGAAAC AGTATCGAGC TGGCCCCTCT 
TTTGAAGAAA GGAAACAGTT GGGCCTGCCC CACCAGAGAC GAGGAGGAAA CTTTAAGTCT 
AAATCCAGGT ACAAGAGAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DDX27 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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