TSC22D2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC22D2
Entrez Gene ID	477111
Full Name	TSC22 domain family member 2
General protein information	Preferred Names TSC22 domain family member 2 Names TSC22 domain family protein 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005634564.2	XP_005634621.1	TSC22 domain family protein 2 isoform X2
XM_534305.6	XP_534305.2	TSC22 domain family protein 2 isoform X1
XM_005634564.3	XP_005634621.1	TSC22 domain family protein 2 isoform X2
XM_534305.5	XP_534305.2	TSC22 domain family protein 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TSC22D2	XP_005634621.1
Mus musculus (house mouse)	Tsc22d2	NP_001074698.1
Xenopus tropicalis (tropical clawed frog)	tsc22d2	XP_004914521.1
Bos taurus (cattle)	TSC22D2	NP_001137573.1
	LOC712879	XP_002808384.1
Homo sapiens (human)	TSC22D2	NP_055594.1
Pan troglodytes (chimpanzee)	TSC22D2	XP_001138557.2
Rattus norvegicus (Norway rat)	Tsc22d2	NP_001178889.1
Gallus gallus (chicken)	LOC100859827	XP_003641833.1

Gene Ontology

The following TSC22D2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC22D2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe24297	XM_534305.6 Latest version!	Canis lupus familiaris TSC22 domain family member 2 (TSC22D2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe24298	XM_005634564.3 Latest version!	Canis lupus familiaris TSC22 domain family member 2 (TSC22D2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe24298	XM_005634564.2	Canis lupus familiaris TSC22 domain family, member 2 (TSC22D2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe24297	XM_534305.5	Canis lupus familiaris TSC22 domain family, member 2 (TSC22D2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC22D2 ( XM_534305.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_534305.2 Canis lupus familiaris TSC22 domain family member 2 (TSC22D2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24297
Clone ID Related Accession (Same CDS sequence)	XM_534305.6 , XM_534305.5
Accession Version	XM_534305.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	TSC22 domain family protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534305.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGGAATCCTT CAACTGCTTT CTACCAAGCG TTCCATTTGA ACACGTTTCA GGAATCAAAG 
AACCTCTGGG ATAGTGCATC TGGGGGAGGT GGTGTTGTAG CCATTGACAA CAAAATAGAA 
CAAGCAATGG ATCTGGTGAA AAGCCATTTG ATGTATGCAG TAAGAGAAGA AGTGGAAGTT 
CTAAAGGAAC AAATAAAAGA ATTAGTTGAA AGAAACTCTT TACTTGAACG AGAAAATGCA 
CTGTTAAAAT CTCTTTCAAA CAATGATCAA TTATCCCAAC TCCCAGCCCA ACAGGCCAAT 
ACTGGTAGCA CTTCTCAACA GCAAGCAGTG ATAGCACAGC CTCCGCAGCC AACGCAACCT 
CCACAGCAGC CGAATGTCTC CTCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534305.2
CDS	1041..3350
Translation	MSKMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDVSSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDGQLAAAAAAPANGGGAAAARSVAGALAGTPAAPSAGAPGAPPAAGSSAGPGTAAPAQPPTTCSSRFRVIKLDHGSGEPYRRGRWTCMEYYERDSDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASVPGAHGPDSGTDSSLTAVSQLPPSEKMSQPAPAQPQSFGVGQPQPPPPVGGAVVQSAAPVPPFPGAAAGPQPMIAAPQPSQPQGTGPGVLPQGPSGQGLPLTNVTLPQQPGPAVGAPATQQPQPFAYPQPQLPPGHLLPVQPAGQSEYLQQHVAGLQPPSPAQPSSTGAGASLPVGAGPTAAAVGAQIMGVASLPGEAVAPGPGPAPGGQAAPGQPAGVPLAALGCAVQPGLVPPGTVQPQSVPPPQMGGSGAPAAVPGGPHAVLPGVPNVPAAAPAPSVPSASTTSITMPNVPAPLAQSQQLSSHTPVSRSSSVIPHGGLPLAPGTPSAPTSLPQADLSQFQTQTQPLVGQADDTRRKSEPLPQPPLSLIAETKPVVKPPVADALANPLQLTPMNSLATSVFSIAIPVDGDEDRNPSTAFYQAFHLNTFQESKNLWDSASGGGGVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELVERNSLLERENALLKSLSNNDQLSQLPAQQANTGSTSQQQAVIAQPPQPTQPPQQPNVSSA

Target ORF information:

RefSeq Version	XM_534305.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC22 domain family member 2 (TSC22D2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534305.6

ORF Insert Sequence:

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGGAATCCTT CAACTGCTTT CTACCAAGCG TTCCATTTGA ACACGTTTCA GGAATCAAAG 
AACCTCTGGG ATAGTGCATC TGGGGGAGGT GGTGTTGTAG CCATTGACAA CAAAATAGAA 
CAAGCAATGG ATCTGGTGAA AAGCCATTTG ATGTATGCAG TAAGAGAAGA AGTGGAAGTT 
CTAAAGGAAC AAATAAAAGA ATTAGTTGAA AGAAACTCTT TACTTGAACG AGAAAATGCA 
CTGTTAAAAT CTCTTTCAAA CAATGATCAA TTATCCCAAC TCCCAGCCCA ACAGGCCAAT 
ACTGGTAGCA CTTCTCAACA GCAAGCAGTG ATAGCACAGC CTCCGCAGCC AACGCAACCT 
CCACAGCAGC CGAATGTCTC CTCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC22D2 ( XM_534305.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_534305.2 Canis lupus familiaris TSC22 domain family, member 2 (TSC22D2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24297
Clone ID Related Accession (Same CDS sequence)	XM_534305.6 , XM_534305.5
Accession Version	XM_534305.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	TSC22 domain family protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534305.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGGAATCCTT CAACTGCTTT CTACCAAGCG TTCCATTTGA ACACGTTTCA GGAATCAAAG 
AACCTCTGGG ATAGTGCATC TGGGGGAGGT GGTGTTGTAG CCATTGACAA CAAAATAGAA 
CAAGCAATGG ATCTGGTGAA AAGCCATTTG ATGTATGCAG TAAGAGAAGA AGTGGAAGTT 
CTAAAGGAAC AAATAAAAGA ATTAGTTGAA AGAAACTCTT TACTTGAACG AGAAAATGCA 
CTGTTAAAAT CTCTTTCAAA CAATGATCAA TTATCCCAAC TCCCAGCCCA ACAGGCCAAT 
ACTGGTAGCA CTTCTCAACA GCAAGCAGTG ATAGCACAGC CTCCGCAGCC AACGCAACCT 
CCACAGCAGC CGAATGTCTC CTCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_534305.2
CDS	99..2408
Translation	MSKMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDVSSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDGQLAAAAAAPANGGGAAAARSVAGALAGTPAAPSAGAPGAPPAAGSSAGPGTAAPAQPPTTCSSRFRVIKLDHGSGEPYRRGRWTCMEYYERDSDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASVPGAHGPDSGTDSSLTAVSQLPPSEKMSQPAPAQPQSFGVGQPQPPPPVGGAVVQSAAPVPPFPGAAAGPQPMIAAPQPSQPQGTGPGVLPQGPSGQGLPLTNVTLPQQPGPAVGAPATQQPQPFAYPQPQLPPGHLLPVQPAGQSEYLQQHVAGLQPPSPAQPSSTGAGASLPVGAGPTAAAVGAQIMGVASLPGEAVAPGPGPAPGGQAAPGQPAGVPLAALGCAVQPGLVPPGTVQPQSVPPPQMGGSGAPAAVPGGPHAVLPGVPNVPAAAPAPSVPSASTTSITMPNVPAPLAQSQQLSSHTPVSRSSSVIPHGGLPLAPGTPSAPTSLPQADLSQFQTQTQPLVGQADDTRRKSEPLPQPPLSLIAETKPVVKPPVADALANPLQLTPMNSLATSVFSIAIPVDGDEDRNPSTAFYQAFHLNTFQESKNLWDSASGGGGVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELVERNSLLERENALLKSLSNNDQLSQLPAQQANTGSTSQQQAVIAQPPQPTQPPQQPNVSSA

Target ORF information:

RefSeq Version	XM_534305.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TSC22 domain family, member 2 (TSC22D2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_534305.5

ORF Insert Sequence:

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGGAATCCTT CAACTGCTTT CTACCAAGCG TTCCATTTGA ACACGTTTCA GGAATCAAAG 
AACCTCTGGG ATAGTGCATC TGGGGGAGGT GGTGTTGTAG CCATTGACAA CAAAATAGAA 
CAAGCAATGG ATCTGGTGAA AAGCCATTTG ATGTATGCAG TAAGAGAAGA AGTGGAAGTT 
CTAAAGGAAC AAATAAAAGA ATTAGTTGAA AGAAACTCTT TACTTGAACG AGAAAATGCA 
CTGTTAAAAT CTCTTTCAAA CAATGATCAA TTATCCCAAC TCCCAGCCCA ACAGGCCAAT 
ACTGGTAGCA CTTCTCAACA GCAAGCAGTG ATAGCACAGC CTCCGCAGCC AACGCAACCT 
CCACAGCAGC CGAATGTCTC CTCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC22D2 ( XM_005634564.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005634621.1 Canis lupus familiaris TSC22 domain family, member 2 (TSC22D2), transcript variant X2, mRNA.

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CloneID	OCe24298
Clone ID Related Accession (Same CDS sequence)	XM_005634564.2 , XM_005634564.3
Accession Version	XM_005634564.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	TSC22 domain family protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGTGCATCTG GGGGAGGTGG TGTTGTAGCC ATTGACAACA AAATAGAACA AGCAATGGAT 
CTGGTGAAAA GCCATTTGAT GTATGCAGTA AGAGAAGAAG TGGAAGTTCT AAAGGAACAA 
ATAAAAGAAT TAGTTGAAAG AAACTCTTTA CTTGAACGAG AAAATGCACT GTTAAAATCT 
CTTTCAAACA ATGATCAATT ATCCCAACTC CCAGCCCAAC AGGCCAATAC TGGTAGCACT 
TCTCAACAGC AAGCAGTGAT AGCACAGCCT CCGCAGCCAA CGCAACCTCC ACAGCAGCCG 
AATGTCTCCT CAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634621.1
CDS	99..2336
Translation	MSKMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDVSSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDGQLAAAAAAPANGGGAAAARSVAGALAGTPAAPSAGAPGAPPAAGSSAGPGTAAPAQPPTTCSSRFRVIKLDHGSGEPYRRGRWTCMEYYERDSDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASVPGAHGPDSGTDSSLTAVSQLPPSEKMSQPAPAQPQSFGVGQPQPPPPVGGAVVQSAAPVPPFPGAAAGPQPMIAAPQPSQPQGTGPGVLPQGPSGQGLPLTNVTLPQQPGPAVGAPATQQPQPFAYPQPQLPPGHLLPVQPAGQSEYLQQHVAGLQPPSPAQPSSTGAGASLPVGAGPTAAAVGAQIMGVASLPGEAVAPGPGPAPGGQAAPGQPAGVPLAALGCAVQPGLVPPGTVQPQSVPPPQMGGSGAPAAVPGGPHAVLPGVPNVPAAAPAPSVPSASTTSITMPNVPAPLAQSQQLSSHTPVSRSSSVIPHGGLPLAPGTPSAPTSLPQADLSQFQTQTQPLVGQADDTRRKSEPLPQPPLSLIAETKPVVKPPVADALANPLQLTPMNSLATSVFSIAIPVDGDEDSASGGGGVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELVERNSLLERENALLKSLSNNDQLSQLPAQQANTGSTSQQQAVIAQPPQPTQPPQQPNVSSA

Target ORF information:

RefSeq Version	XM_005634564.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TSC22 domain family, member 2 (TSC22D2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634564.2

ORF Insert Sequence:

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGTGCATCTG GGGGAGGTGG TGTTGTAGCC ATTGACAACA AAATAGAACA AGCAATGGAT 
CTGGTGAAAA GCCATTTGAT GTATGCAGTA AGAGAAGAAG TGGAAGTTCT AAAGGAACAA 
ATAAAAGAAT TAGTTGAAAG AAACTCTTTA CTTGAACGAG AAAATGCACT GTTAAAATCT 
CTTTCAAACA ATGATCAATT ATCCCAACTC CCAGCCCAAC AGGCCAATAC TGGTAGCACT 
TCTCAACAGC AAGCAGTGAT AGCACAGCCT CCGCAGCCAA CGCAACCTCC ACAGCAGCCG 
AATGTCTCCT CAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC22D2 ( XM_005634564.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005634621.1 Canis lupus familiaris TSC22 domain family member 2 (TSC22D2), transcript variant X2, mRNA.

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CloneID	OCe24298
Clone ID Related Accession (Same CDS sequence)	XM_005634564.2 , XM_005634564.3
Accession Version	XM_005634564.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	TSC22 domain family protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGTGCATCTG GGGGAGGTGG TGTTGTAGCC ATTGACAACA AAATAGAACA AGCAATGGAT 
CTGGTGAAAA GCCATTTGAT GTATGCAGTA AGAGAAGAAG TGGAAGTTCT AAAGGAACAA 
ATAAAAGAAT TAGTTGAAAG AAACTCTTTA CTTGAACGAG AAAATGCACT GTTAAAATCT 
CTTTCAAACA ATGATCAATT ATCCCAACTC CCAGCCCAAC AGGCCAATAC TGGTAGCACT 
TCTCAACAGC AAGCAGTGAT AGCACAGCCT CCGCAGCCAA CGCAACCTCC ACAGCAGCCG 
AATGTCTCCT CAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634621.1
CDS	1040..3277
Translation	MSKMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDVSSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDGQLAAAAAAPANGGGAAAARSVAGALAGTPAAPSAGAPGAPPAAGSSAGPGTAAPAQPPTTCSSRFRVIKLDHGSGEPYRRGRWTCMEYYERDSDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASVPGAHGPDSGTDSSLTAVSQLPPSEKMSQPAPAQPQSFGVGQPQPPPPVGGAVVQSAAPVPPFPGAAAGPQPMIAAPQPSQPQGTGPGVLPQGPSGQGLPLTNVTLPQQPGPAVGAPATQQPQPFAYPQPQLPPGHLLPVQPAGQSEYLQQHVAGLQPPSPAQPSSTGAGASLPVGAGPTAAAVGAQIMGVASLPGEAVAPGPGPAPGGQAAPGQPAGVPLAALGCAVQPGLVPPGTVQPQSVPPPQMGGSGAPAAVPGGPHAVLPGVPNVPAAAPAPSVPSASTTSITMPNVPAPLAQSQQLSSHTPVSRSSSVIPHGGLPLAPGTPSAPTSLPQADLSQFQTQTQPLVGQADDTRRKSEPLPQPPLSLIAETKPVVKPPVADALANPLQLTPMNSLATSVFSIAIPVDGDEDSASGGGGVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELVERNSLLERENALLKSLSNNDQLSQLPAQQANTGSTSQQQAVIAQPPQPTQPPQQPNVSSA

Target ORF information:

RefSeq Version	XM_005634564.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC22 domain family member 2 (TSC22D2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634564.3

ORF Insert Sequence:

ATGTCCAAGA TGCCGGCCAA GAAGAAGAGC TGCTTCCAGA TCACCAGTGT CACCACCGCC 
CAGGTGGCCA CTAGCATCAC CGAGGACACC GAGAGCCTGG ACGACCCGGA CGAGTCGCGC 
ACAGAGGACG TGTCCTCCGA GATCTTCGAC GTGTCTCGGG CCACCGACTA CGGCCCCGAG 
GAGGTCTGCG AGCGCAGCTC CTCCGAAGAG ACGCTCAACA ATGTTGGGGA TGCGGAGACT 
CCCGGGACCG TGTCCCCGAA CCTCCTGCTG GACGGACAGC TGGCGGCCGC GGCGGCGGCT 
CCCGCCAACG GAGGAGGAGC CGCGGCGGCC CGGAGCGTGG CCGGCGCGCT GGCCGGTACC 
CCGGCGGCCC CCTCGGCGGG GGCACCGGGC GCCCCCCCGG CGGCGGGCTC GTCCGCCGGG 
CCTGGGACTG CGGCCCCAGC ACAGCCCCCC ACCACTTGCA GTTCCCGTTT TCGAGTCATC 
AAGCTGGACC ACGGGAGCGG GGAGCCCTAC CGACGCGGCC GATGGACGTG CATGGAATAC 
TATGAGCGGG ATTCAGACAG CAGCGTCCTG ACCAGGTCCG GGGATTGCAT TAGGCACAGC 
AGTACTTTCG ACCAGACTGC GGAGCGGGAC AGCGGCCTCG GCGCCACCGG AGGGTCGGTG 
GTGGTGGTGG TGGCCTCCGT GCCGGGGGCG CACGGGCCCG ACTCGGGAAC TGACAGTTCC 
CTGACTGCTG TGTCACAGCT ACCCCCGTCG GAGAAAATGA GCCAGCCCGC CCCGGCCCAG 
CCGCAGAGTT TTGGCGTTGG GCAGCCACAG CCACCGCCGC CCGTAGGTGG GGCTGTGGTT 
CAAAGCGCGG CTCCGGTGCC GCCGTTCCCG GGAGCCGCGG CCGGGCCACA GCCAATGATC 
GCGGCCCCGC AGCCAAGCCA GCCCCAGGGA ACCGGCCCCG GCGTCCTCCC GCAGGGCCCC 
AGTGGACAAG GGCTGCCGCT GACGAATGTA ACCCTGCCGC AGCAGCCGGG CCCCGCGGTC 
GGCGCTCCTG CGACGCAGCA GCCCCAGCCG TTCGCGTACC CCCAGCCTCA GCTACCGCCC 
GGGCATTTGC TGCCAGTCCA GCCCGCGGGG CAGAGTGAGT ACCTACAGCA GCACGTGGCC 
GGCCTGCAGC CGCCAAGCCC CGCGCAGCCC TCGTCCACCG GCGCCGGAGC GAGCCTTCCC 
GTGGGCGCCG GCCCGACTGC CGCCGCGGTG GGGGCGCAGA TAATGGGCGT GGCTTCCCTG 
CCCGGCGAGG CCGTGGCCCC GGGGCCGGGA CCCGCGCCCG GAGGACAGGC GGCGCCCGGT 
CAGCCGGCCG GAGTGCCCCT GGCTGCTCTT GGATGCGCGG TGCAGCCGGG CCTGGTCCCC 
CCTGGGACCG TGCAGCCCCA GTCCGTGCCT CCGCCGCAGA TGGGTGGCAG TGGGGCGCCG 
GCAGCCGTGC CCGGCGGCCC CCACGCCGTT CTACCCGGAG TTCCAAACGT GCCTGCAGCC 
GCGCCCGCTC CAAGCGTGCC TAGTGCGTCT ACCACCTCCA TTACTATGCC AAATGTCCCC 
GCGCCGCTGG CCCAGTCGCA GCAGCTGAGC AGCCATACGC CCGTCAGCCG GAGCAGCAGC 
GTAATCCCGC ACGGTGGGCT GCCCTTAGCG CCAGGCACAC CCAGTGCACC GACGAGTCTA 
CCGCAGGCTG ACCTAAGCCA GTTCCAGACT CAGACCCAGC CTTTGGTCGG GCAAGCCGAC 
GATACCAGAA GAAAATCAGA ACCCCTACCT CAACCACCAC TTTCTCTCAT TGCTGAAACT 
AAGCCTGTCG TGAAGCCTCC TGTTGCAGAT GCCCTGGCCA ACCCCCTTCA GTTAACACCT 
ATGAACAGTC TCGCCACCTC TGTGTTCAGC ATAGCTATTC CCGTGGATGG CGATGAAGAC 
AGTGCATCTG GGGGAGGTGG TGTTGTAGCC ATTGACAACA AAATAGAACA AGCAATGGAT 
CTGGTGAAAA GCCATTTGAT GTATGCAGTA AGAGAAGAAG TGGAAGTTCT AAAGGAACAA 
ATAAAAGAAT TAGTTGAAAG AAACTCTTTA CTTGAACGAG AAAATGCACT GTTAAAATCT 
CTTTCAAACA ATGATCAATT ATCCCAACTC CCAGCCCAAC AGGCCAATAC TGGTAGCACT 
TCTCAACAGC AAGCAGTGAT AGCACAGCCT CCGCAGCCAA CGCAACCTCC ACAGCAGCCG 
AATGTCTCCT CAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TSC22D2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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