SEMA4D cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA4D
Entrez Gene ID	476350
Full Name	semaphorin 4D
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_533551.6	XP_533551.4	semaphorin-4D isoform X2
XM_533551.5	XP_533551.4	semaphorin-4D isoform X2
XM_005615960.2	XP_005616017.1	semaphorin-4D isoform X4
XM_005615959.2	XP_005616016.1	semaphorin-4D isoform X3
XM_005615960.3	XP_005616017.1	semaphorin-4D isoform X3
XM_014117969.1	XP_013973444.1	semaphorin-4D isoform X5
XM_005615958.2	XP_005616015.1	semaphorin-4D isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sema4d	NP_001164034.1
Homo sapiens (human)	SEMA4D	NP_006369.3
Pan troglodytes (chimpanzee)	SEMA4D	XP_001141771.2
Canis lupus familiaris (dog)	SEMA4D	XP_005616015.1
Gallus gallus (chicken)	SEMA4D	XP_424426.4
Danio rerio (zebrafish)	sema4d	XP_003200859.2
	SEMA4D	XP_001090890.1
Mus musculus (house mouse)	Sema4d	NP_038688.2

Gene Ontology

The following SEMA4D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA4D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe01443	XM_005615960.3 Latest version!	Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe01440	XM_005615958.2 Latest version!	Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe01441	XM_533551.6 Latest version!	Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe01442	XM_005615959.2 Latest version!	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe34845	XM_014117969.1 Latest version!	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OCe01441	XM_533551.5	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe01443	XM_005615960.2	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA4D ( XM_005615960.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005616017.1 Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe01443
Clone ID Related Accession (Same CDS sequence)	XM_005615960.2 , XM_005615960.3
Accession Version	XM_005615960.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	semaphorin-4D isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TTGCTCCGGC GCTGCTGTCA TGCTCGTGGG TGAAGCCAGT GAAACGGGCC 
TTTCATCACC CGCTGCTGGG GCGTCAGCCG CGTTCTCGTC TACATGCCCG TCCCCAGGCT 
CTCCCCTCGG CCCGCTGCTG TCTGGGGCAC ACACGGCCTG GGCGCGGCAT TGGGATGGCC 
TGGCCGGCCC AGGGCGGCAA GCCCCGGCTC CCCTCTGGCC CCTCCAGACC CCGTCTGTGC 
GCTGACCTCC CCTGCTGGGC ACTGGGGCCT CCGCATGGGG TGTGCACTCC GGTGTCGGGG 
AGGATGGAGA GACCGCGGGA CCAAGCCCAG GCTTCTGAGG ACACCGTCCT GGGAACAACA 
GGGGGCAGGT CCTGGAAGGA GAAGATTCAG AATTTCTTCG TGAGGCCGCA GAGAAGCGCA 
CCATTGAAGA ACCGGGTGGC CCTCGACATC TGCGGTGGGG ACCTCACCCA GTGCCTGGGA 
GATGCGGGTC CTCAGCATTC ACACCATCCA CCCGCTCCGG GCCGTGCTGC AGGTCGTGGC 
CCCCCGGCTC TGAGCAGCAT TGGCCGTCAC CCCCACCACG GGCTCCCTGC CCACGAGCCT 
TCACTACCGG AGCCCCCCCC GTGGCGCCTG CGCGTGAGAA TGTGCGCCCC CATCGGTGGG 
CTGCTTGCCG CCCTGGCCAT GGCATTTGGG ATGACGGTGG CATTCGCACC GGTCCCCAGG 
ATCACCTGGG AGCACAGAGA GGTGCAGCTG GAGAAGTTTC ACGAGCCCGG CATCTTCAAC 
TACTCGGTGT TGCTGCTAAG TGAGGACAAG AGCACCCTGT ACGTGGGTGC CCGGGAGGCC 
GTGTTTGCCC TGAGCGCACT CAACGTCTCC AAGAAGCAGC ATGAGGCGTA TTGGAAGGTC 
TCGGAAGATA AAAAAGCAAA ATGTGCAGAA AAGGGCAAAT CAAGACAGAC AGAATGTCTC 
AACTACATCC GCGTGCTGCA GCCACTCAGT GCCAGCGCCC TGTACGTGTG CGGGACCAAC 
GCGTTCCAGC CAGCCTGCGA CCACCTGAAC TTAACGTCCT TTCAGTTTCT GGGGCGAACA 
GAAGATGGAA AGGGCAGGTG CCCCTTCGAC CCCGCACAGA GCTACACTTC TGTCATGGTT 
GACGGGGAGC TGTATTCTGG GACGTCCTAC AACTTTCTGG GAAGTGAGCC CATCATCTCC 
CGAAACTCGT CCCATAGCCC TCTGAGGACG GAGTACGCGA TCCCGTGGCT CAACGAGCCA 
AGCTTCGTCT TTGCTGACGT GATCCGAGAA CACCCAGATG GCGTGGACAG CGGGGATGAC 
CGGGTCTACT TCTTCTTCAC CGAGGTGTCT GTGGAGTATG AGTTTGTGTT CAAGCTGATG 
ATCCCACGAG TGGCCAGGGT GTGCAAGGGG GACCAGGGTG GCCTGCGGAC CCTGCAGAAG 
AAGTGGACGT CTTTCCTCAA GGCCAGGCTG ATCTGTTCCC GGCCAGACAA CAACCTCGTC 
TTCAACGTGC TGCAGGACAT CTTTGTCCTC AGGTCCCCAG ACCTCAAGGA GCCCGTGTTC 
TACGGGGTCT TCACCCCACA GCTGAACAAC GTGGGGCTGT CGGCTGTGTG TGCCTACAAC 
CTGTCCACGG CCGAGGCGGT CTTCTCCGGC GGGAAGTACA TGCAGAGCGC CACGGTGGAG 
CAGTCCCACA CCAAGTGGGT GCGCTACAAC GGGGCCGTGC CCACGCCGCG GCCCGGGGCG 
TGCATCAACC GTGAAGCGCG GGCCGCCAAC TTCTCCAGCT CCCTCACTCT GCCGGACAAG 
ACCTTACAGT TCATCAAAGA CCACCCTCTC ATGGACGGCT CCGTGACCCC GATAGACAAC 
AGGCCCAGGC TGATCAAGAG AGACGTGAAC TACACGCAGA TCGTGGTGGA CAGGACACGG 
GCCCTGGACG GCACCGTCTA TGACGTCATG TTTGTCAGCA CAGACCGGGG TGCCCTGCAC 
AAAGCCGTCA GCTTCGAGCA TGAGGCCCAC ATCATCGAGG AGACGCAGCT CTTCCCGGAC 
TTTGAGCCGA TCCAGACGCT GCTGCTGAAT TCCCAGCAGG GCAAGAGATT TGTGTACGCG 
GGTTCCAGCT CGGGTGTGGT GCAGGCCCCC GTGGCCTTCT GCGGGAAGCA CGGGAGCTGC 
GAGGACTGCG TGCTGGCCCG CGACCCCTAC TGCGCCTGGA GCCGTGCCGC CGAGGCCTGC 
GTGGACCTGC ACCGGACAGA CAGCCCCAGC AGGACTTTGG TTCAGCAGAT GAGCGGTGAC 
ACGTCCGTCT GCCCCACCTC ACCTCTGCCC TTCCCCCCTC CGGGGTCCTC CTCCCTGTCC 
TGTCGGGGCC CCGGCTCAGC TTCCTCTCCC CGAAGCCCCT GGCCGGCCTC GGCCTCGGGG 
CCTGACAGCA GCCTGCCGGT TGCCCTCCTG CCACCCTTCC TCAGCGACCA GGCACAGCAC 
GTGCACGCCC TGGGGACCTT CCACCTCTTC TGCCAGGCCA CAGGTCCTGC AGACGTTCAC 
TTTGTCTGGG AGAAGAATGG CCGTGAGCTG GAGACATGTG TCCCCACACA GACCCACGGG 
CTGCACGACG GCAGGACCCA CGTGCTCAGC TGGCTGCGAG ATGCCCTCCA AGAGAGCGCC 
GAGTACCGCT GCTCCGTGTT GTCTTCCGCG GGCAACAAGA CCTCTGGAGT GCGGGTCACC 
GTCATGAGAC ATGAAGCGAC CCACCAGGAG CAGTGGACCA GAGAGCTCGC CGCGTGGAGG 
GCTGTGGCCG GCGAGCATGA CCGCATGATG CGCAGCTGGA GGAAGGCGTG GGAAAGCTGT 
GACAAGGACA CCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616017.1
CDS	16..2853
Translation	MARFAPALLSCSWVKPVKRAFHHPLLGRQPRSRLHARPQALPSARCCLGHTRPGRGIGMAWPAQGGKPRLPSGPSRPRLCADLPCWALGPPHGVCTPVSGRMERPRDQAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPTSPLPFPPPGSSSLSCRGPGSASSPRSPWPASASGPDSSLPVALLPPFLSDQAQHVHALGTFHLFCQATGPADVHFVWEKNGRELETCVPTQTHGLHDGRTHVLSWLRDALQESAEYRCSVLSSAGNKTSGVRVTVMRHEATHQEQWTRELAAWRAVAGEHDRMMRSWRKAWESCDKDTF

Target ORF information:

RefSeq Version	XM_005615960.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615960.2

ORF Insert Sequence:

ATGGCCCGTT TTGCTCCGGC GCTGCTGTCA TGCTCGTGGG TGAAGCCAGT GAAACGGGCC 
TTTCATCACC CGCTGCTGGG GCGTCAGCCG CGTTCTCGTC TACATGCCCG TCCCCAGGCT 
CTCCCCTCGG CCCGCTGCTG TCTGGGGCAC ACACGGCCTG GGCGCGGCAT TGGGATGGCC 
TGGCCGGCCC AGGGCGGCAA GCCCCGGCTC CCCTCTGGCC CCTCCAGACC CCGTCTGTGC 
GCTGACCTCC CCTGCTGGGC ACTGGGGCCT CCGCATGGGG TGTGCACTCC GGTGTCGGGG 
AGGATGGAGA GACCGCGGGA CCAAGCCCAG GCTTCTGAGG ACACCGTCCT GGGAACAACA 
GGGGGCAGGT CCTGGAAGGA GAAGATTCAG AATTTCTTCG TGAGGCCGCA GAGAAGCGCA 
CCATTGAAGA ACCGGGTGGC CCTCGACATC TGCGGTGGGG ACCTCACCCA GTGCCTGGGA 
GATGCGGGTC CTCAGCATTC ACACCATCCA CCCGCTCCGG GCCGTGCTGC AGGTCGTGGC 
CCCCCGGCTC TGAGCAGCAT TGGCCGTCAC CCCCACCACG GGCTCCCTGC CCACGAGCCT 
TCACTACCGG AGCCCCCCCC GTGGCGCCTG CGCGTGAGAA TGTGCGCCCC CATCGGTGGG 
CTGCTTGCCG CCCTGGCCAT GGCATTTGGG ATGACGGTGG CATTCGCACC GGTCCCCAGG 
ATCACCTGGG AGCACAGAGA GGTGCAGCTG GAGAAGTTTC ACGAGCCCGG CATCTTCAAC 
TACTCGGTGT TGCTGCTAAG TGAGGACAAG AGCACCCTGT ACGTGGGTGC CCGGGAGGCC 
GTGTTTGCCC TGAGCGCACT CAACGTCTCC AAGAAGCAGC ATGAGGCGTA TTGGAAGGTC 
TCGGAAGATA AAAAAGCAAA ATGTGCAGAA AAGGGCAAAT CAAGACAGAC AGAATGTCTC 
AACTACATCC GCGTGCTGCA GCCACTCAGT GCCAGCGCCC TGTACGTGTG CGGGACCAAC 
GCGTTCCAGC CAGCCTGCGA CCACCTGAAC TTAACGTCCT TTCAGTTTCT GGGGCGAACA 
GAAGATGGAA AGGGCAGGTG CCCCTTCGAC CCCGCACAGA GCTACACTTC TGTCATGGTT 
GACGGGGAGC TGTATTCTGG GACGTCCTAC AACTTTCTGG GAAGTGAGCC CATCATCTCC 
CGAAACTCGT CCCATAGCCC TCTGAGGACG GAGTACGCGA TCCCGTGGCT CAACGAGCCA 
AGCTTCGTCT TTGCTGACGT GATCCGAGAA CACCCAGATG GCGTGGACAG CGGGGATGAC 
CGGGTCTACT TCTTCTTCAC CGAGGTGTCT GTGGAGTATG AGTTTGTGTT CAAGCTGATG 
ATCCCACGAG TGGCCAGGGT GTGCAAGGGG GACCAGGGTG GCCTGCGGAC CCTGCAGAAG 
AAGTGGACGT CTTTCCTCAA GGCCAGGCTG ATCTGTTCCC GGCCAGACAA CAACCTCGTC 
TTCAACGTGC TGCAGGACAT CTTTGTCCTC AGGTCCCCAG ACCTCAAGGA GCCCGTGTTC 
TACGGGGTCT TCACCCCACA GCTGAACAAC GTGGGGCTGT CGGCTGTGTG TGCCTACAAC 
CTGTCCACGG CCGAGGCGGT CTTCTCCGGC GGGAAGTACA TGCAGAGCGC CACGGTGGAG 
CAGTCCCACA CCAAGTGGGT GCGCTACAAC GGGGCCGTGC CCACGCCGCG GCCCGGGGCG 
TGCATCAACC GTGAAGCGCG GGCCGCCAAC TTCTCCAGCT CCCTCACTCT GCCGGACAAG 
ACCTTACAGT TCATCAAAGA CCACCCTCTC ATGGACGGCT CCGTGACCCC GATAGACAAC 
AGGCCCAGGC TGATCAAGAG AGACGTGAAC TACACGCAGA TCGTGGTGGA CAGGACACGG 
GCCCTGGACG GCACCGTCTA TGACGTCATG TTTGTCAGCA CAGACCGGGG TGCCCTGCAC 
AAAGCCGTCA GCTTCGAGCA TGAGGCCCAC ATCATCGAGG AGACGCAGCT CTTCCCGGAC 
TTTGAGCCGA TCCAGACGCT GCTGCTGAAT TCCCAGCAGG GCAAGAGATT TGTGTACGCG 
GGTTCCAGCT CGGGTGTGGT GCAGGCCCCC GTGGCCTTCT GCGGGAAGCA CGGGAGCTGC 
GAGGACTGCG TGCTGGCCCG CGACCCCTAC TGCGCCTGGA GCCGTGCCGC CGAGGCCTGC 
GTGGACCTGC ACCGGACAGA CAGCCCCAGC AGGACTTTGG TTCAGCAGAT GAGCGGTGAC 
ACGTCCGTCT GCCCCACCTC ACCTCTGCCC TTCCCCCCTC CGGGGTCCTC CTCCCTGTCC 
TGTCGGGGCC CCGGCTCAGC TTCCTCTCCC CGAAGCCCCT GGCCGGCCTC GGCCTCGGGG 
CCTGACAGCA GCCTGCCGGT TGCCCTCCTG CCACCCTTCC TCAGCGACCA GGCACAGCAC 
GTGCACGCCC TGGGGACCTT CCACCTCTTC TGCCAGGCCA CAGGTCCTGC AGACGTTCAC 
TTTGTCTGGG AGAAGAATGG CCGTGAGCTG GAGACATGTG TCCCCACACA GACCCACGGG 
CTGCACGACG GCAGGACCCA CGTGCTCAGC TGGCTGCGAG ATGCCCTCCA AGAGAGCGCC 
GAGTACCGCT GCTCCGTGTT GTCTTCCGCG GGCAACAAGA CCTCTGGAGT GCGGGTCACC 
GTCATGAGAC ATGAAGCGAC CCACCAGGAG CAGTGGACCA GAGAGCTCGC CGCGTGGAGG 
GCTGTGGCCG GCGAGCATGA CCGCATGATG CGCAGCTGGA GGAAGGCGTG GGAAAGCTGT 
GACAAGGACA CCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4D ( XM_005615960.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005616017.1 Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe01443
Clone ID Related Accession (Same CDS sequence)	XM_005615960.2 , XM_005615960.3
Accession Version	XM_005615960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	semaphorin-4D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TTGCTCCGGC GCTGCTGTCA TGCTCGTGGG TGAAGCCAGT GAAACGGGCC 
TTTCATCACC CGCTGCTGGG GCGTCAGCCG CGTTCTCGTC TACATGCCCG TCCCCAGGCT 
CTCCCCTCGG CCCGCTGCTG TCTGGGGCAC ACACGGCCTG GGCGCGGCAT TGGGATGGCC 
TGGCCGGCCC AGGGCGGCAA GCCCCGGCTC CCCTCTGGCC CCTCCAGACC CCGTCTGTGC 
GCTGACCTCC CCTGCTGGGC ACTGGGGCCT CCGCATGGGG TGTGCACTCC GGTGTCGGGG 
AGGATGGAGA GACCGCGGGA CCAAGCCCAG GCTTCTGAGG ACACCGTCCT GGGAACAACA 
GGGGGCAGGT CCTGGAAGGA GAAGATTCAG AATTTCTTCG TGAGGCCGCA GAGAAGCGCA 
CCATTGAAGA ACCGGGTGGC CCTCGACATC TGCGGTGGGG ACCTCACCCA GTGCCTGGGA 
GATGCGGGTC CTCAGCATTC ACACCATCCA CCCGCTCCGG GCCGTGCTGC AGGTCGTGGC 
CCCCCGGCTC TGAGCAGCAT TGGCCGTCAC CCCCACCACG GGCTCCCTGC CCACGAGCCT 
TCACTACCGG AGCCCCCCCC GTGGCGCCTG CGCGTGAGAA TGTGCGCCCC CATCGGTGGG 
CTGCTTGCCG CCCTGGCCAT GGCATTTGGG ATGACGGTGG CATTCGCACC GGTCCCCAGG 
ATCACCTGGG AGCACAGAGA GGTGCAGCTG GAGAAGTTTC ACGAGCCCGG CATCTTCAAC 
TACTCGGTGT TGCTGCTAAG TGAGGACAAG AGCACCCTGT ACGTGGGTGC CCGGGAGGCC 
GTGTTTGCCC TGAGCGCACT CAACGTCTCC AAGAAGCAGC ATGAGGCGTA TTGGAAGGTC 
TCGGAAGATA AAAAAGCAAA ATGTGCAGAA AAGGGCAAAT CAAGACAGAC AGAATGTCTC 
AACTACATCC GCGTGCTGCA GCCACTCAGT GCCAGCGCCC TGTACGTGTG CGGGACCAAC 
GCGTTCCAGC CAGCCTGCGA CCACCTGAAC TTAACGTCCT TTCAGTTTCT GGGGCGAACA 
GAAGATGGAA AGGGCAGGTG CCCCTTCGAC CCCGCACAGA GCTACACTTC TGTCATGGTT 
GACGGGGAGC TGTATTCTGG GACGTCCTAC AACTTTCTGG GAAGTGAGCC CATCATCTCC 
CGAAACTCGT CCCATAGCCC TCTGAGGACG GAGTACGCGA TCCCGTGGCT CAACGAGCCA 
AGCTTCGTCT TTGCTGACGT GATCCGAGAA CACCCAGATG GCGTGGACAG CGGGGATGAC 
CGGGTCTACT TCTTCTTCAC CGAGGTGTCT GTGGAGTATG AGTTTGTGTT CAAGCTGATG 
ATCCCACGAG TGGCCAGGGT GTGCAAGGGG GACCAGGGTG GCCTGCGGAC CCTGCAGAAG 
AAGTGGACGT CTTTCCTCAA GGCCAGGCTG ATCTGTTCCC GGCCAGACAA CAACCTCGTC 
TTCAACGTGC TGCAGGACAT CTTTGTCCTC AGGTCCCCAG ACCTCAAGGA GCCCGTGTTC 
TACGGGGTCT TCACCCCACA GCTGAACAAC GTGGGGCTGT CGGCTGTGTG TGCCTACAAC 
CTGTCCACGG CCGAGGCGGT CTTCTCCGGC GGGAAGTACA TGCAGAGCGC CACGGTGGAG 
CAGTCCCACA CCAAGTGGGT GCGCTACAAC GGGGCCGTGC CCACGCCGCG GCCCGGGGCG 
TGCATCAACC GTGAAGCGCG GGCCGCCAAC TTCTCCAGCT CCCTCACTCT GCCGGACAAG 
ACCTTACAGT TCATCAAAGA CCACCCTCTC ATGGACGGCT CCGTGACCCC GATAGACAAC 
AGGCCCAGGC TGATCAAGAG AGACGTGAAC TACACGCAGA TCGTGGTGGA CAGGACACGG 
GCCCTGGACG GCACCGTCTA TGACGTCATG TTTGTCAGCA CAGACCGGGG TGCCCTGCAC 
AAAGCCGTCA GCTTCGAGCA TGAGGCCCAC ATCATCGAGG AGACGCAGCT CTTCCCGGAC 
TTTGAGCCGA TCCAGACGCT GCTGCTGAAT TCCCAGCAGG GCAAGAGATT TGTGTACGCG 
GGTTCCAGCT CGGGTGTGGT GCAGGCCCCC GTGGCCTTCT GCGGGAAGCA CGGGAGCTGC 
GAGGACTGCG TGCTGGCCCG CGACCCCTAC TGCGCCTGGA GCCGTGCCGC CGAGGCCTGC 
GTGGACCTGC ACCGGACAGA CAGCCCCAGC AGGACTTTGG TTCAGCAGAT GAGCGGTGAC 
ACGTCCGTCT GCCCCACCTC ACCTCTGCCC TTCCCCCCTC CGGGGTCCTC CTCCCTGTCC 
TGTCGGGGCC CCGGCTCAGC TTCCTCTCCC CGAAGCCCCT GGCCGGCCTC GGCCTCGGGG 
CCTGACAGCA GCCTGCCGGT TGCCCTCCTG CCACCCTTCC TCAGCGACCA GGCACAGCAC 
GTGCACGCCC TGGGGACCTT CCACCTCTTC TGCCAGGCCA CAGGTCCTGC AGACGTTCAC 
TTTGTCTGGG AGAAGAATGG CCGTGAGCTG GAGACATGTG TCCCCACACA GACCCACGGG 
CTGCACGACG GCAGGACCCA CGTGCTCAGC TGGCTGCGAG ATGCCCTCCA AGAGAGCGCC 
GAGTACCGCT GCTCCGTGTT GTCTTCCGCG GGCAACAAGA CCTCTGGAGT GCGGGTCACC 
GTCATGAGAC ATGAAGCGAC CCACCAGGAG CAGTGGACCA GAGAGCTCGC CGCGTGGAGG 
GCTGTGGCCG GCGAGCATGA CCGCATGATG CGCAGCTGGA GGAAGGCGTG GGAAAGCTGT 
GACAAGGACA CCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616017.1
CDS	16..2853
Translation	MARFAPALLSCSWVKPVKRAFHHPLLGRQPRSRLHARPQALPSARCCLGHTRPGRGIGMAWPAQGGKPRLPSGPSRPRLCADLPCWALGPPHGVCTPVSGRMERPRDQAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPTSPLPFPPPGSSSLSCRGPGSASSPRSPWPASASGPDSSLPVALLPPFLSDQAQHVHALGTFHLFCQATGPADVHFVWEKNGRELETCVPTQTHGLHDGRTHVLSWLRDALQESAEYRCSVLSSAGNKTSGVRVTVMRHEATHQEQWTRELAAWRAVAGEHDRMMRSWRKAWESCDKDTF

Target ORF information:

RefSeq Version	XM_005615960.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615960.3

ORF Insert Sequence:

ATGGCCCGTT TTGCTCCGGC GCTGCTGTCA TGCTCGTGGG TGAAGCCAGT GAAACGGGCC 
TTTCATCACC CGCTGCTGGG GCGTCAGCCG CGTTCTCGTC TACATGCCCG TCCCCAGGCT 
CTCCCCTCGG CCCGCTGCTG TCTGGGGCAC ACACGGCCTG GGCGCGGCAT TGGGATGGCC 
TGGCCGGCCC AGGGCGGCAA GCCCCGGCTC CCCTCTGGCC CCTCCAGACC CCGTCTGTGC 
GCTGACCTCC CCTGCTGGGC ACTGGGGCCT CCGCATGGGG TGTGCACTCC GGTGTCGGGG 
AGGATGGAGA GACCGCGGGA CCAAGCCCAG GCTTCTGAGG ACACCGTCCT GGGAACAACA 
GGGGGCAGGT CCTGGAAGGA GAAGATTCAG AATTTCTTCG TGAGGCCGCA GAGAAGCGCA 
CCATTGAAGA ACCGGGTGGC CCTCGACATC TGCGGTGGGG ACCTCACCCA GTGCCTGGGA 
GATGCGGGTC CTCAGCATTC ACACCATCCA CCCGCTCCGG GCCGTGCTGC AGGTCGTGGC 
CCCCCGGCTC TGAGCAGCAT TGGCCGTCAC CCCCACCACG GGCTCCCTGC CCACGAGCCT 
TCACTACCGG AGCCCCCCCC GTGGCGCCTG CGCGTGAGAA TGTGCGCCCC CATCGGTGGG 
CTGCTTGCCG CCCTGGCCAT GGCATTTGGG ATGACGGTGG CATTCGCACC GGTCCCCAGG 
ATCACCTGGG AGCACAGAGA GGTGCAGCTG GAGAAGTTTC ACGAGCCCGG CATCTTCAAC 
TACTCGGTGT TGCTGCTAAG TGAGGACAAG AGCACCCTGT ACGTGGGTGC CCGGGAGGCC 
GTGTTTGCCC TGAGCGCACT CAACGTCTCC AAGAAGCAGC ATGAGGCGTA TTGGAAGGTC 
TCGGAAGATA AAAAAGCAAA ATGTGCAGAA AAGGGCAAAT CAAGACAGAC AGAATGTCTC 
AACTACATCC GCGTGCTGCA GCCACTCAGT GCCAGCGCCC TGTACGTGTG CGGGACCAAC 
GCGTTCCAGC CAGCCTGCGA CCACCTGAAC TTAACGTCCT TTCAGTTTCT GGGGCGAACA 
GAAGATGGAA AGGGCAGGTG CCCCTTCGAC CCCGCACAGA GCTACACTTC TGTCATGGTT 
GACGGGGAGC TGTATTCTGG GACGTCCTAC AACTTTCTGG GAAGTGAGCC CATCATCTCC 
CGAAACTCGT CCCATAGCCC TCTGAGGACG GAGTACGCGA TCCCGTGGCT CAACGAGCCA 
AGCTTCGTCT TTGCTGACGT GATCCGAGAA CACCCAGATG GCGTGGACAG CGGGGATGAC 
CGGGTCTACT TCTTCTTCAC CGAGGTGTCT GTGGAGTATG AGTTTGTGTT CAAGCTGATG 
ATCCCACGAG TGGCCAGGGT GTGCAAGGGG GACCAGGGTG GCCTGCGGAC CCTGCAGAAG 
AAGTGGACGT CTTTCCTCAA GGCCAGGCTG ATCTGTTCCC GGCCAGACAA CAACCTCGTC 
TTCAACGTGC TGCAGGACAT CTTTGTCCTC AGGTCCCCAG ACCTCAAGGA GCCCGTGTTC 
TACGGGGTCT TCACCCCACA GCTGAACAAC GTGGGGCTGT CGGCTGTGTG TGCCTACAAC 
CTGTCCACGG CCGAGGCGGT CTTCTCCGGC GGGAAGTACA TGCAGAGCGC CACGGTGGAG 
CAGTCCCACA CCAAGTGGGT GCGCTACAAC GGGGCCGTGC CCACGCCGCG GCCCGGGGCG 
TGCATCAACC GTGAAGCGCG GGCCGCCAAC TTCTCCAGCT CCCTCACTCT GCCGGACAAG 
ACCTTACAGT TCATCAAAGA CCACCCTCTC ATGGACGGCT CCGTGACCCC GATAGACAAC 
AGGCCCAGGC TGATCAAGAG AGACGTGAAC TACACGCAGA TCGTGGTGGA CAGGACACGG 
GCCCTGGACG GCACCGTCTA TGACGTCATG TTTGTCAGCA CAGACCGGGG TGCCCTGCAC 
AAAGCCGTCA GCTTCGAGCA TGAGGCCCAC ATCATCGAGG AGACGCAGCT CTTCCCGGAC 
TTTGAGCCGA TCCAGACGCT GCTGCTGAAT TCCCAGCAGG GCAAGAGATT TGTGTACGCG 
GGTTCCAGCT CGGGTGTGGT GCAGGCCCCC GTGGCCTTCT GCGGGAAGCA CGGGAGCTGC 
GAGGACTGCG TGCTGGCCCG CGACCCCTAC TGCGCCTGGA GCCGTGCCGC CGAGGCCTGC 
GTGGACCTGC ACCGGACAGA CAGCCCCAGC AGGACTTTGG TTCAGCAGAT GAGCGGTGAC 
ACGTCCGTCT GCCCCACCTC ACCTCTGCCC TTCCCCCCTC CGGGGTCCTC CTCCCTGTCC 
TGTCGGGGCC CCGGCTCAGC TTCCTCTCCC CGAAGCCCCT GGCCGGCCTC GGCCTCGGGG 
CCTGACAGCA GCCTGCCGGT TGCCCTCCTG CCACCCTTCC TCAGCGACCA GGCACAGCAC 
GTGCACGCCC TGGGGACCTT CCACCTCTTC TGCCAGGCCA CAGGTCCTGC AGACGTTCAC 
TTTGTCTGGG AGAAGAATGG CCGTGAGCTG GAGACATGTG TCCCCACACA GACCCACGGG 
CTGCACGACG GCAGGACCCA CGTGCTCAGC TGGCTGCGAG ATGCCCTCCA AGAGAGCGCC 
GAGTACCGCT GCTCCGTGTT GTCTTCCGCG GGCAACAAGA CCTCTGGAGT GCGGGTCACC 
GTCATGAGAC ATGAAGCGAC CCACCAGGAG CAGTGGACCA GAGAGCTCGC CGCGTGGAGG 
GCTGTGGCCG GCGAGCATGA CCGCATGATG CGCAGCTGGA GGAAGGCGTG GGAAAGCTGT 
GACAAGGACA CCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4D ( XM_005615958.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005616015.1 Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe01440
Clone ID Related Accession (Same CDS sequence)	XM_005615958.2
Accession Version	XM_005615958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	semaphorin-4D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TTGCTCCGGC GCTGCTGTCA TGCTCGTGGG TGAAGCCAGT GAAACGGGCC 
TTTCATCACC CGCTGCTGGG GCGTCAGCCG CGTTCTCGTC TACATGCCCG TCCCCAGGCT 
CTCCCCTCGG CCCGCTGCTG TCTGGGGCAC ACACGGCCTG GGCGCGGCAT TGGGATGGCC 
TGGCCGGCCC AGGGCGGCAA GCCCCGGCTC CCCTCTGGCC CCTCCAGACC CCGTCTGTGC 
GCTGACCTCC CCTGCTGGGC ACTGGGGCCT CCGCATGGGG TGTGCACTCC GGTGTCGGGG 
AGGATGGAGA GACCGCGGGA CCAAGCCCAG GCTTCTGAGG ACACCGTCCT GGGAACAACA 
GGGGGCAGGT CCTGGAAGGA GAAGATTCAG AATTTCTTCG TGAGGCCGCA GAGAAGCGCA 
CCATTGAAGA ACCGGGTGGC CCTCGACATC TGCGGTGGGG ACCTCACCCA GTGCCTGGGA 
GATGCGGGTC CTCAGCATTC ACACCATCCA CCCGCTCCGG GCCGTGCTGC AGGTCGTGGC 
CCCCCGGCTC TGAGCAGCAT TGGCCGTCAC CCCCACCACG GGCTCCCTGC CCACGAGCCT 
TCACTACCGG AGCCCCCCCC GTGGCGCCTG CGCGTGAGAA TGTGCGCCCC CATCGGTGGG 
CTGCTTGCCG CCCTGGCCAT GGCATTTGGG ATGACGGTGG CATTCGCACC GGTCCCCAGG 
ATCACCTGGG AGCACAGAGA GGTGCAGCTG GAGAAGTTTC ACGAGCCCGG CATCTTCAAC 
TACTCGGTGT TGCTGCTAAG TGAGGACAAG AGCACCCTGT ACGTGGGTGC CCGGGAGGCC 
GTGTTTGCCC TGAGCGCACT CAACGTCTCC AAGAAGCAGC ATGAGGCGTA TTGGAAGGTC 
TCGGAAGATA AAAAAGCAAA ATGTGCAGAA AAGGGCAAAT CAAGACAGAC AGAATGTCTC 
AACTACATCC GCGTGCTGCA GCCACTCAGT GCCAGCGCCC TGTACGTGTG CGGGACCAAC 
GCGTTCCAGC CAGCCTGCGA CCACCTGAAC TTAACGTCCT TTCAGTTTCT GGGGCGAACA 
GAAGATGGAA AGGGCAGGTG CCCCTTCGAC CCCGCACAGA GCTACACTTC TGTCATGGTT 
GACGGGGAGC TGTATTCTGG GACGTCCTAC AACTTTCTGG GAAGTGAGCC CATCATCTCC 
CGAAACTCGT CCCATAGCCC TCTGAGGACG GAGTACGCGA TCCCGTGGCT CAACGAGCCA 
AGCTTCGTCT TTGCTGACGT GATCCGAGAA CACCCAGATG GCGTGGACAG CGGGGATGAC 
CGGGTCTACT TCTTCTTCAC CGAGGTGTCT GTGGAGTATG AGTTTGTGTT CAAGCTGATG 
ATCCCACGAG TGGCCAGGGT GTGCAAGGGG GACCAGGGTG GCCTGCGGAC CCTGCAGAAG 
AAGTGGACGT CTTTCCTCAA GGCCAGGCTG ATCTGTTCCC GGCCAGACAA CAACCTCGTC 
TTCAACGTGC TGCAGGACAT CTTTGTCCTC AGGTCCCCAG ACCTCAAGGA GCCCGTGTTC 
TACGGGGTCT TCACCCCACA GCTGAACAAC GTGGGGCTGT CGGCTGTGTG TGCCTACAAC 
CTGTCCACGG CCGAGGCGGT CTTCTCCGGC GGGAAGTACA TGCAGAGCGC CACGGTGGAG 
CAGTCCCACA CCAAGTGGGT GCGCTACAAC GGGGCCGTGC CCACGCCGCG GCCCGGGGCG 
TGCATCAACC GTGAAGCGCG GGCCGCCAAC TTCTCCAGCT CCCTCACTCT GCCGGACAAG 
ACCTTACAGT TCATCAAAGA CCACCCTCTC ATGGACGGCT CCGTGACCCC GATAGACAAC 
AGGCCCAGGC TGATCAAGAG AGACGTGAAC TACACGCAGA TCGTGGTGGA CAGGACACGG 
GCCCTGGACG GCACCGTCTA TGACGTCATG TTTGTCAGCA CAGACCGGGG TGCCCTGCAC 
AAAGCCGTCA GCTTCGAGCA TGAGGCCCAC ATCATCGAGG AGACGCAGCT CTTCCCGGAC 
TTTGAGCCGA TCCAGACGCT GCTGCTGAAT TCCCAGCAGG GCAAGAGATT TGTGTACGCG 
GGTTCCAGCT CGGGTGTGGT GCAGGCCCCC GTGGCCTTCT GCGGGAAGCA CGGGAGCTGC 
GAGGACTGCG TGCTGGCCCG CGACCCCTAC TGCGCCTGGA GCCGTGCCGC CGAGGCCTGC 
GTGGACCTGC ACCGGACAGA CAGCCCCAGC AGGACTTTGG TTCAGCAGAT GAGCGGTGAC 
ACGTCCGTCT GCCCCAATGA AACTAAAAGT TTCCGGGAGC ATTTTTTCAA GCACGGCGGC 
ACAGCAGAAC TGAAATGCTC CCAAAAGTCG AACCTGGCCC GGGTGGTGTG GACGTTCCGG 
GACCGCGTAC TCAAGGCCCA GACCCCCAAG TATGGCCTGG TGGGCAGGAG AGACTTGCTC 
ATCTTCGACC TGTCAGAGGG AGACAGCGGG GTGTACCAGT GCCTGTCGGA GGAGAGAGTC 
AGGAACAGGA CGGTCCTGCA GCTGCTGGCC AAGCACGTTC TGGAAGTCAG GGTGGTTCCT 
AGGACCCCGG CTGCCTCCAC CCCACCAGCC GCCCGGACGG AAGGCGACAG GACCATCCTC 
AGAGTGTCGA CGGAGCCCAC CCCAGGCCCC CCGCCCCAGA CCCCGGCCAT GCGGGGCCCC 
ACGCCCAGCC CCGCGCCCAC CGGCGGCACC TGCAAACCAA AGACCATCAT CAACACGGTC 
CCTGAGGTCC ACTCGGAGAA GACCATGTAT CTCAAGTCCA GTGACAACCG CCTGCTCATG 
TTCCTCTTCC TCTTCTTCTT CGTGCTCTGC CTCGGCCTCT TTTCCTACAA CTGCTACAAG 
GGCTACCTGC CCGGCCACTG CCTGAAGCTC CGCTCCGCCA TGCTGCTGGG GAAGAAGCAG 
CCCGCGTCCG ACTTCTCCGA CTTTGAGCAG AGCGTGAAGG AGACGCTGGT GGAGCAGGGC 
AGCTTCTCGC AGCAGAACGG GGGGCAGCCC CGGCCGGCCC TGGACACCGG CTACGAGACG 
GAGCAGGACA CCATGGCCAG CCGGGTGCCC ACCGACAGGG AAGACTCGCA GAGGCTCGAC 
GACCTGCCGG TCCGGGACAG GCCGTTTGAC GTCAAGTGTG AGCTCAAGTA TGCCGACTCG 
GACGCGGACG TGGACGCGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616015.1
CDS	16..3219
Translation	MARFAPALLSCSWVKPVKRAFHHPLLGRQPRSRLHARPQALPSARCCLGHTRPGRGIGMAWPAQGGKPRLPSGPSRPRLCADLPCWALGPPHGVCTPVSGRMERPRDQAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPNETKSFREHFFKHGGTAELKCSQKSNLARVVWTFRDRVLKAQTPKYGLVGRRDLLIFDLSEGDSGVYQCLSEERVRNRTVLQLLAKHVLEVRVVPRTPAASTPPAARTEGDRTILRVSTEPTPGPPPQTPAMRGPTPSPAPTGGTCKPKTIINTVPEVHSEKTMYLKSSDNRLLMFLFLFFFVLCLGLFSYNCYKGYLPGHCLKLRSAMLLGKKQPASDFSDFEQSVKETLVEQGSFSQQNGGQPRPALDTGYETEQDTMASRVPTDREDSQRLDDLPVRDRPFDVKCELKYADSDADVDAE

Target ORF information:

RefSeq Version	XM_005615958.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615958.2

ORF Insert Sequence:

ATGGCCCGTT TTGCTCCGGC GCTGCTGTCA TGCTCGTGGG TGAAGCCAGT GAAACGGGCC 
TTTCATCACC CGCTGCTGGG GCGTCAGCCG CGTTCTCGTC TACATGCCCG TCCCCAGGCT 
CTCCCCTCGG CCCGCTGCTG TCTGGGGCAC ACACGGCCTG GGCGCGGCAT TGGGATGGCC 
TGGCCGGCCC AGGGCGGCAA GCCCCGGCTC CCCTCTGGCC CCTCCAGACC CCGTCTGTGC 
GCTGACCTCC CCTGCTGGGC ACTGGGGCCT CCGCATGGGG TGTGCACTCC GGTGTCGGGG 
AGGATGGAGA GACCGCGGGA CCAAGCCCAG GCTTCTGAGG ACACCGTCCT GGGAACAACA 
GGGGGCAGGT CCTGGAAGGA GAAGATTCAG AATTTCTTCG TGAGGCCGCA GAGAAGCGCA 
CCATTGAAGA ACCGGGTGGC CCTCGACATC TGCGGTGGGG ACCTCACCCA GTGCCTGGGA 
GATGCGGGTC CTCAGCATTC ACACCATCCA CCCGCTCCGG GCCGTGCTGC AGGTCGTGGC 
CCCCCGGCTC TGAGCAGCAT TGGCCGTCAC CCCCACCACG GGCTCCCTGC CCACGAGCCT 
TCACTACCGG AGCCCCCCCC GTGGCGCCTG CGCGTGAGAA TGTGCGCCCC CATCGGTGGG 
CTGCTTGCCG CCCTGGCCAT GGCATTTGGG ATGACGGTGG CATTCGCACC GGTCCCCAGG 
ATCACCTGGG AGCACAGAGA GGTGCAGCTG GAGAAGTTTC ACGAGCCCGG CATCTTCAAC 
TACTCGGTGT TGCTGCTAAG TGAGGACAAG AGCACCCTGT ACGTGGGTGC CCGGGAGGCC 
GTGTTTGCCC TGAGCGCACT CAACGTCTCC AAGAAGCAGC ATGAGGCGTA TTGGAAGGTC 
TCGGAAGATA AAAAAGCAAA ATGTGCAGAA AAGGGCAAAT CAAGACAGAC AGAATGTCTC 
AACTACATCC GCGTGCTGCA GCCACTCAGT GCCAGCGCCC TGTACGTGTG CGGGACCAAC 
GCGTTCCAGC CAGCCTGCGA CCACCTGAAC TTAACGTCCT TTCAGTTTCT GGGGCGAACA 
GAAGATGGAA AGGGCAGGTG CCCCTTCGAC CCCGCACAGA GCTACACTTC TGTCATGGTT 
GACGGGGAGC TGTATTCTGG GACGTCCTAC AACTTTCTGG GAAGTGAGCC CATCATCTCC 
CGAAACTCGT CCCATAGCCC TCTGAGGACG GAGTACGCGA TCCCGTGGCT CAACGAGCCA 
AGCTTCGTCT TTGCTGACGT GATCCGAGAA CACCCAGATG GCGTGGACAG CGGGGATGAC 
CGGGTCTACT TCTTCTTCAC CGAGGTGTCT GTGGAGTATG AGTTTGTGTT CAAGCTGATG 
ATCCCACGAG TGGCCAGGGT GTGCAAGGGG GACCAGGGTG GCCTGCGGAC CCTGCAGAAG 
AAGTGGACGT CTTTCCTCAA GGCCAGGCTG ATCTGTTCCC GGCCAGACAA CAACCTCGTC 
TTCAACGTGC TGCAGGACAT CTTTGTCCTC AGGTCCCCAG ACCTCAAGGA GCCCGTGTTC 
TACGGGGTCT TCACCCCACA GCTGAACAAC GTGGGGCTGT CGGCTGTGTG TGCCTACAAC 
CTGTCCACGG CCGAGGCGGT CTTCTCCGGC GGGAAGTACA TGCAGAGCGC CACGGTGGAG 
CAGTCCCACA CCAAGTGGGT GCGCTACAAC GGGGCCGTGC CCACGCCGCG GCCCGGGGCG 
TGCATCAACC GTGAAGCGCG GGCCGCCAAC TTCTCCAGCT CCCTCACTCT GCCGGACAAG 
ACCTTACAGT TCATCAAAGA CCACCCTCTC ATGGACGGCT CCGTGACCCC GATAGACAAC 
AGGCCCAGGC TGATCAAGAG AGACGTGAAC TACACGCAGA TCGTGGTGGA CAGGACACGG 
GCCCTGGACG GCACCGTCTA TGACGTCATG TTTGTCAGCA CAGACCGGGG TGCCCTGCAC 
AAAGCCGTCA GCTTCGAGCA TGAGGCCCAC ATCATCGAGG AGACGCAGCT CTTCCCGGAC 
TTTGAGCCGA TCCAGACGCT GCTGCTGAAT TCCCAGCAGG GCAAGAGATT TGTGTACGCG 
GGTTCCAGCT CGGGTGTGGT GCAGGCCCCC GTGGCCTTCT GCGGGAAGCA CGGGAGCTGC 
GAGGACTGCG TGCTGGCCCG CGACCCCTAC TGCGCCTGGA GCCGTGCCGC CGAGGCCTGC 
GTGGACCTGC ACCGGACAGA CAGCCCCAGC AGGACTTTGG TTCAGCAGAT GAGCGGTGAC 
ACGTCCGTCT GCCCCAATGA AACTAAAAGT TTCCGGGAGC ATTTTTTCAA GCACGGCGGC 
ACAGCAGAAC TGAAATGCTC CCAAAAGTCG AACCTGGCCC GGGTGGTGTG GACGTTCCGG 
GACCGCGTAC TCAAGGCCCA GACCCCCAAG TATGGCCTGG TGGGCAGGAG AGACTTGCTC 
ATCTTCGACC TGTCAGAGGG AGACAGCGGG GTGTACCAGT GCCTGTCGGA GGAGAGAGTC 
AGGAACAGGA CGGTCCTGCA GCTGCTGGCC AAGCACGTTC TGGAAGTCAG GGTGGTTCCT 
AGGACCCCGG CTGCCTCCAC CCCACCAGCC GCCCGGACGG AAGGCGACAG GACCATCCTC 
AGAGTGTCGA CGGAGCCCAC CCCAGGCCCC CCGCCCCAGA CCCCGGCCAT GCGGGGCCCC 
ACGCCCAGCC CCGCGCCCAC CGGCGGCACC TGCAAACCAA AGACCATCAT CAACACGGTC 
CCTGAGGTCC ACTCGGAGAA GACCATGTAT CTCAAGTCCA GTGACAACCG CCTGCTCATG 
TTCCTCTTCC TCTTCTTCTT CGTGCTCTGC CTCGGCCTCT TTTCCTACAA CTGCTACAAG 
GGCTACCTGC CCGGCCACTG CCTGAAGCTC CGCTCCGCCA TGCTGCTGGG GAAGAAGCAG 
CCCGCGTCCG ACTTCTCCGA CTTTGAGCAG AGCGTGAAGG AGACGCTGGT GGAGCAGGGC 
AGCTTCTCGC AGCAGAACGG GGGGCAGCCC CGGCCGGCCC TGGACACCGG CTACGAGACG 
GAGCAGGACA CCATGGCCAG CCGGGTGCCC ACCGACAGGG AAGACTCGCA GAGGCTCGAC 
GACCTGCCGG TCCGGGACAG GCCGTTTGAC GTCAAGTGTG AGCTCAAGTA TGCCGACTCG 
GACGCGGACG TGGACGCGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4D ( XM_533551.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533551.4 Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe01441
Clone ID Related Accession (Same CDS sequence)	XM_533551.6 , XM_533551.5
Accession Version	XM_533551.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	semaphorin-4D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533551.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACTT TCCCAGTCTT GTCTCTTGGG TGGAGAGCCC AGGCTTCTGA GGACACCGTC 
CTGGGAACAA CAGGGGGCAG GTCCTGGAAG GAGAAGATTC AGAATTTCTT CGTGAGGCCG 
CAGAGAAGCG CACCATTGAA GAACCGGGTG GCCCTCGACA TCTGCGGTGG GGACCTCACC 
CAGTGCCTGG GAGATGCGGG TCCTCAGCAT TCACACCATC CACCCGCTCC GGGCCGTGCT 
GCAGGTCGTG GCCCCCCGGC TCTGAGCAGC ATTGGCCGTC ACCCCCACCA CGGGCTCCCT 
GCCCACGAGC CTTCACTACC GGAGCCCCCC CCGTGGCGCC TGCGCGTGAG AATGTGCGCC 
CCCATCGGTG GGCTGCTTGC CGCCCTGGCC ATGGCATTTG GGATGACGGT GGCATTCGCA 
CCGGTCCCCA GGATCACCTG GGAGCACAGA GAGGTGCAGC TGGAGAAGTT TCACGAGCCC 
GGCATCTTCA ACTACTCGGT GTTGCTGCTA AGTGAGGACA AGAGCACCCT GTACGTGGGT 
GCCCGGGAGG CCGTGTTTGC CCTGAGCGCA CTCAACGTCT CCAAGAAGCA GCATGAGGCG 
TATTGGAAGG TCTCGGAAGA TAAAAAAGCA AAATGTGCAG AAAAGGGCAA ATCAAGACAG 
ACAGAATGTC TCAACTACAT CCGCGTGCTG CAGCCACTCA GTGCCAGCGC CCTGTACGTG 
TGCGGGACCA ACGCGTTCCA GCCAGCCTGC GACCACCTGA ACTTAACGTC CTTTCAGTTT 
CTGGGGCGAA CAGAAGATGG AAAGGGCAGG TGCCCCTTCG ACCCCGCACA GAGCTACACT 
TCTGTCATGG TTGACGGGGA GCTGTATTCT GGGACGTCCT ACAACTTTCT GGGAAGTGAG 
CCCATCATCT CCCGAAACTC GTCCCATAGC CCTCTGAGGA CGGAGTACGC GATCCCGTGG 
CTCAACGAGC CAAGCTTCGT CTTTGCTGAC GTGATCCGAG AACACCCAGA TGGCGTGGAC 
AGCGGGGATG ACCGGGTCTA CTTCTTCTTC ACCGAGGTGT CTGTGGAGTA TGAGTTTGTG 
TTCAAGCTGA TGATCCCACG AGTGGCCAGG GTGTGCAAGG GGGACCAGGG TGGCCTGCGG 
ACCCTGCAGA AGAAGTGGAC GTCTTTCCTC AAGGCCAGGC TGATCTGTTC CCGGCCAGAC 
AACAACCTCG TCTTCAACGT GCTGCAGGAC ATCTTTGTCC TCAGGTCCCC AGACCTCAAG 
GAGCCCGTGT TCTACGGGGT CTTCACCCCA CAGCTGAACA ACGTGGGGCT GTCGGCTGTG 
TGTGCCTACA ACCTGTCCAC GGCCGAGGCG GTCTTCTCCG GCGGGAAGTA CATGCAGAGC 
GCCACGGTGG AGCAGTCCCA CACCAAGTGG GTGCGCTACA ACGGGGCCGT GCCCACGCCG 
CGGCCCGGGG CGTGCATCAA CCGTGAAGCG CGGGCCGCCA ACTTCTCCAG CTCCCTCACT 
CTGCCGGACA AGACCTTACA GTTCATCAAA GACCACCCTC TCATGGACGG CTCCGTGACC 
CCGATAGACA ACAGGCCCAG GCTGATCAAG AGAGACGTGA ACTACACGCA GATCGTGGTG 
GACAGGACAC GGGCCCTGGA CGGCACCGTC TATGACGTCA TGTTTGTCAG CACAGACCGG 
GGTGCCCTGC ACAAAGCCGT CAGCTTCGAG CATGAGGCCC ACATCATCGA GGAGACGCAG 
CTCTTCCCGG ACTTTGAGCC GATCCAGACG CTGCTGCTGA ATTCCCAGCA GGGCAAGAGA 
TTTGTGTACG CGGGTTCCAG CTCGGGTGTG GTGCAGGCCC CCGTGGCCTT CTGCGGGAAG 
CACGGGAGCT GCGAGGACTG CGTGCTGGCC CGCGACCCCT ACTGCGCCTG GAGCCGTGCC 
GCCGAGGCCT GCGTGGACCT GCACCGGACA GACAGCCCCA GCAGGACTTT GGTTCAGCAG 
ATGAGCGGTG ACACGTCCGT CTGCCCCAAT GAAACTAAAA GTTTCCGGGA GCATTTTTTC 
AAGCACGGCG GCACAGCAGA ACTGAAATGC TCCCAAAAGT CGAACCTGGC CCGGGTGGTG 
TGGACGTTCC GGGACCGCGT ACTCAAGGCC CAGACCCCCA AGTATGGCCT GGTGGGCAGG 
AGAGACTTGC TCATCTTCGA CCTGTCAGAG GGAGACAGCG GGGTGTACCA GTGCCTGTCG 
GAGGAGAGAG TCAGGAACAG GACGGTCCTG CAGCTGCTGG CCAAGCACGT TCTGGAAGTC 
AGGGTGGTTC CTAGGACCCC GGCTGCCTCC ACCCCACCAG CCGCCCGGAC GGAAGGCGAC 
AGGACCATCC TCAGAGTGTC GACGGAGCCC ACCCCAGGCC CCCCGCCCCA GACCCCGGCC 
ATGCGGGGCC CCACGCCCAG CCCCGCGCCC ACCGGCGGCA CCTGCAAACC AAAGACCATC 
ATCAACACGG TCCCTGAGGT CCACTCGGAG AAGACCATGT ATCTCAAGTC CAGTGACAAC 
CGCCTGCTCA TGTTCCTCTT CCTCTTCTTC TTCGTGCTCT GCCTCGGCCT CTTTTCCTAC 
AACTGCTACA AGGGCTACCT GCCCGGCCAC TGCCTGAAGC TCCGCTCCGC CATGCTGCTG 
GGGAAGAAGC AGCCCGCGTC CGACTTCTCC GACTTTGAGC AGAGCGTGAA GGAGACGCTG 
GTGGAGCAGG GCAGCTTCTC GCAGCAGAAC GGGGGGCAGC CCCGGCCGGC CCTGGACACC 
GGCTACGAGA CGGAGCAGGA CACCATGGCC AGCCGGGTGC CCACCGACAG GGAAGACTCG 
CAGAGGCTCG ACGACCTGCC GGTCCGGGAC AGGCCGTTTG ACGTCAAGTG TGAGCTCAAG 
TATGCCGACT CGGACGCGGA CGTGGACGCG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533551.4
CDS	16..2931
Translation	MWTFPVLSLGWRAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPNETKSFREHFFKHGGTAELKCSQKSNLARVVWTFRDRVLKAQTPKYGLVGRRDLLIFDLSEGDSGVYQCLSEERVRNRTVLQLLAKHVLEVRVVPRTPAASTPPAARTEGDRTILRVSTEPTPGPPPQTPAMRGPTPSPAPTGGTCKPKTIINTVPEVHSEKTMYLKSSDNRLLMFLFLFFFVLCLGLFSYNCYKGYLPGHCLKLRSAMLLGKKQPASDFSDFEQSVKETLVEQGSFSQQNGGQPRPALDTGYETEQDTMASRVPTDREDSQRLDDLPVRDRPFDVKCELKYADSDADVDAE

Target ORF information:

RefSeq Version	XM_533551.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris semaphorin 4D (SEMA4D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533551.6

ORF Insert Sequence:

ATGTGGACTT TCCCAGTCTT GTCTCTTGGG TGGAGAGCCC AGGCTTCTGA GGACACCGTC 
CTGGGAACAA CAGGGGGCAG GTCCTGGAAG GAGAAGATTC AGAATTTCTT CGTGAGGCCG 
CAGAGAAGCG CACCATTGAA GAACCGGGTG GCCCTCGACA TCTGCGGTGG GGACCTCACC 
CAGTGCCTGG GAGATGCGGG TCCTCAGCAT TCACACCATC CACCCGCTCC GGGCCGTGCT 
GCAGGTCGTG GCCCCCCGGC TCTGAGCAGC ATTGGCCGTC ACCCCCACCA CGGGCTCCCT 
GCCCACGAGC CTTCACTACC GGAGCCCCCC CCGTGGCGCC TGCGCGTGAG AATGTGCGCC 
CCCATCGGTG GGCTGCTTGC CGCCCTGGCC ATGGCATTTG GGATGACGGT GGCATTCGCA 
CCGGTCCCCA GGATCACCTG GGAGCACAGA GAGGTGCAGC TGGAGAAGTT TCACGAGCCC 
GGCATCTTCA ACTACTCGGT GTTGCTGCTA AGTGAGGACA AGAGCACCCT GTACGTGGGT 
GCCCGGGAGG CCGTGTTTGC CCTGAGCGCA CTCAACGTCT CCAAGAAGCA GCATGAGGCG 
TATTGGAAGG TCTCGGAAGA TAAAAAAGCA AAATGTGCAG AAAAGGGCAA ATCAAGACAG 
ACAGAATGTC TCAACTACAT CCGCGTGCTG CAGCCACTCA GTGCCAGCGC CCTGTACGTG 
TGCGGGACCA ACGCGTTCCA GCCAGCCTGC GACCACCTGA ACTTAACGTC CTTTCAGTTT 
CTGGGGCGAA CAGAAGATGG AAAGGGCAGG TGCCCCTTCG ACCCCGCACA GAGCTACACT 
TCTGTCATGG TTGACGGGGA GCTGTATTCT GGGACGTCCT ACAACTTTCT GGGAAGTGAG 
CCCATCATCT CCCGAAACTC GTCCCATAGC CCTCTGAGGA CGGAGTACGC GATCCCGTGG 
CTCAACGAGC CAAGCTTCGT CTTTGCTGAC GTGATCCGAG AACACCCAGA TGGCGTGGAC 
AGCGGGGATG ACCGGGTCTA CTTCTTCTTC ACCGAGGTGT CTGTGGAGTA TGAGTTTGTG 
TTCAAGCTGA TGATCCCACG AGTGGCCAGG GTGTGCAAGG GGGACCAGGG TGGCCTGCGG 
ACCCTGCAGA AGAAGTGGAC GTCTTTCCTC AAGGCCAGGC TGATCTGTTC CCGGCCAGAC 
AACAACCTCG TCTTCAACGT GCTGCAGGAC ATCTTTGTCC TCAGGTCCCC AGACCTCAAG 
GAGCCCGTGT TCTACGGGGT CTTCACCCCA CAGCTGAACA ACGTGGGGCT GTCGGCTGTG 
TGTGCCTACA ACCTGTCCAC GGCCGAGGCG GTCTTCTCCG GCGGGAAGTA CATGCAGAGC 
GCCACGGTGG AGCAGTCCCA CACCAAGTGG GTGCGCTACA ACGGGGCCGT GCCCACGCCG 
CGGCCCGGGG CGTGCATCAA CCGTGAAGCG CGGGCCGCCA ACTTCTCCAG CTCCCTCACT 
CTGCCGGACA AGACCTTACA GTTCATCAAA GACCACCCTC TCATGGACGG CTCCGTGACC 
CCGATAGACA ACAGGCCCAG GCTGATCAAG AGAGACGTGA ACTACACGCA GATCGTGGTG 
GACAGGACAC GGGCCCTGGA CGGCACCGTC TATGACGTCA TGTTTGTCAG CACAGACCGG 
GGTGCCCTGC ACAAAGCCGT CAGCTTCGAG CATGAGGCCC ACATCATCGA GGAGACGCAG 
CTCTTCCCGG ACTTTGAGCC GATCCAGACG CTGCTGCTGA ATTCCCAGCA GGGCAAGAGA 
TTTGTGTACG CGGGTTCCAG CTCGGGTGTG GTGCAGGCCC CCGTGGCCTT CTGCGGGAAG 
CACGGGAGCT GCGAGGACTG CGTGCTGGCC CGCGACCCCT ACTGCGCCTG GAGCCGTGCC 
GCCGAGGCCT GCGTGGACCT GCACCGGACA GACAGCCCCA GCAGGACTTT GGTTCAGCAG 
ATGAGCGGTG ACACGTCCGT CTGCCCCAAT GAAACTAAAA GTTTCCGGGA GCATTTTTTC 
AAGCACGGCG GCACAGCAGA ACTGAAATGC TCCCAAAAGT CGAACCTGGC CCGGGTGGTG 
TGGACGTTCC GGGACCGCGT ACTCAAGGCC CAGACCCCCA AGTATGGCCT GGTGGGCAGG 
AGAGACTTGC TCATCTTCGA CCTGTCAGAG GGAGACAGCG GGGTGTACCA GTGCCTGTCG 
GAGGAGAGAG TCAGGAACAG GACGGTCCTG CAGCTGCTGG CCAAGCACGT TCTGGAAGTC 
AGGGTGGTTC CTAGGACCCC GGCTGCCTCC ACCCCACCAG CCGCCCGGAC GGAAGGCGAC 
AGGACCATCC TCAGAGTGTC GACGGAGCCC ACCCCAGGCC CCCCGCCCCA GACCCCGGCC 
ATGCGGGGCC CCACGCCCAG CCCCGCGCCC ACCGGCGGCA CCTGCAAACC AAAGACCATC 
ATCAACACGG TCCCTGAGGT CCACTCGGAG AAGACCATGT ATCTCAAGTC CAGTGACAAC 
CGCCTGCTCA TGTTCCTCTT CCTCTTCTTC TTCGTGCTCT GCCTCGGCCT CTTTTCCTAC 
AACTGCTACA AGGGCTACCT GCCCGGCCAC TGCCTGAAGC TCCGCTCCGC CATGCTGCTG 
GGGAAGAAGC AGCCCGCGTC CGACTTCTCC GACTTTGAGC AGAGCGTGAA GGAGACGCTG 
GTGGAGCAGG GCAGCTTCTC GCAGCAGAAC GGGGGGCAGC CCCGGCCGGC CCTGGACACC 
GGCTACGAGA CGGAGCAGGA CACCATGGCC AGCCGGGTGC CCACCGACAG GGAAGACTCG 
CAGAGGCTCG ACGACCTGCC GGTCCGGGAC AGGCCGTTTG ACGTCAAGTG TGAGCTCAAG 
TATGCCGACT CGGACGCGGA CGTGGACGCG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4D ( XM_533551.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533551.4 Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe01441
Clone ID Related Accession (Same CDS sequence)	XM_533551.6 , XM_533551.5
Accession Version	XM_533551.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	semaphorin-4D isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533551.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACTT TCCCAGTCTT GTCTCTTGGG TGGAGAGCCC AGGCTTCTGA GGACACCGTC 
CTGGGAACAA CAGGGGGCAG GTCCTGGAAG GAGAAGATTC AGAATTTCTT CGTGAGGCCG 
CAGAGAAGCG CACCATTGAA GAACCGGGTG GCCCTCGACA TCTGCGGTGG GGACCTCACC 
CAGTGCCTGG GAGATGCGGG TCCTCAGCAT TCACACCATC CACCCGCTCC GGGCCGTGCT 
GCAGGTCGTG GCCCCCCGGC TCTGAGCAGC ATTGGCCGTC ACCCCCACCA CGGGCTCCCT 
GCCCACGAGC CTTCACTACC GGAGCCCCCC CCGTGGCGCC TGCGCGTGAG AATGTGCGCC 
CCCATCGGTG GGCTGCTTGC CGCCCTGGCC ATGGCATTTG GGATGACGGT GGCATTCGCA 
CCGGTCCCCA GGATCACCTG GGAGCACAGA GAGGTGCAGC TGGAGAAGTT TCACGAGCCC 
GGCATCTTCA ACTACTCGGT GTTGCTGCTA AGTGAGGACA AGAGCACCCT GTACGTGGGT 
GCCCGGGAGG CCGTGTTTGC CCTGAGCGCA CTCAACGTCT CCAAGAAGCA GCATGAGGCG 
TATTGGAAGG TCTCGGAAGA TAAAAAAGCA AAATGTGCAG AAAAGGGCAA ATCAAGACAG 
ACAGAATGTC TCAACTACAT CCGCGTGCTG CAGCCACTCA GTGCCAGCGC CCTGTACGTG 
TGCGGGACCA ACGCGTTCCA GCCAGCCTGC GACCACCTGA ACTTAACGTC CTTTCAGTTT 
CTGGGGCGAA CAGAAGATGG AAAGGGCAGG TGCCCCTTCG ACCCCGCACA GAGCTACACT 
TCTGTCATGG TTGACGGGGA GCTGTATTCT GGGACGTCCT ACAACTTTCT GGGAAGTGAG 
CCCATCATCT CCCGAAACTC GTCCCATAGC CCTCTGAGGA CGGAGTACGC GATCCCGTGG 
CTCAACGAGC CAAGCTTCGT CTTTGCTGAC GTGATCCGAG AACACCCAGA TGGCGTGGAC 
AGCGGGGATG ACCGGGTCTA CTTCTTCTTC ACCGAGGTGT CTGTGGAGTA TGAGTTTGTG 
TTCAAGCTGA TGATCCCACG AGTGGCCAGG GTGTGCAAGG GGGACCAGGG TGGCCTGCGG 
ACCCTGCAGA AGAAGTGGAC GTCTTTCCTC AAGGCCAGGC TGATCTGTTC CCGGCCAGAC 
AACAACCTCG TCTTCAACGT GCTGCAGGAC ATCTTTGTCC TCAGGTCCCC AGACCTCAAG 
GAGCCCGTGT TCTACGGGGT CTTCACCCCA CAGCTGAACA ACGTGGGGCT GTCGGCTGTG 
TGTGCCTACA ACCTGTCCAC GGCCGAGGCG GTCTTCTCCG GCGGGAAGTA CATGCAGAGC 
GCCACGGTGG AGCAGTCCCA CACCAAGTGG GTGCGCTACA ACGGGGCCGT GCCCACGCCG 
CGGCCCGGGG CGTGCATCAA CCGTGAAGCG CGGGCCGCCA ACTTCTCCAG CTCCCTCACT 
CTGCCGGACA AGACCTTACA GTTCATCAAA GACCACCCTC TCATGGACGG CTCCGTGACC 
CCGATAGACA ACAGGCCCAG GCTGATCAAG AGAGACGTGA ACTACACGCA GATCGTGGTG 
GACAGGACAC GGGCCCTGGA CGGCACCGTC TATGACGTCA TGTTTGTCAG CACAGACCGG 
GGTGCCCTGC ACAAAGCCGT CAGCTTCGAG CATGAGGCCC ACATCATCGA GGAGACGCAG 
CTCTTCCCGG ACTTTGAGCC GATCCAGACG CTGCTGCTGA ATTCCCAGCA GGGCAAGAGA 
TTTGTGTACG CGGGTTCCAG CTCGGGTGTG GTGCAGGCCC CCGTGGCCTT CTGCGGGAAG 
CACGGGAGCT GCGAGGACTG CGTGCTGGCC CGCGACCCCT ACTGCGCCTG GAGCCGTGCC 
GCCGAGGCCT GCGTGGACCT GCACCGGACA GACAGCCCCA GCAGGACTTT GGTTCAGCAG 
ATGAGCGGTG ACACGTCCGT CTGCCCCAAT GAAACTAAAA GTTTCCGGGA GCATTTTTTC 
AAGCACGGCG GCACAGCAGA ACTGAAATGC TCCCAAAAGT CGAACCTGGC CCGGGTGGTG 
TGGACGTTCC GGGACCGCGT ACTCAAGGCC CAGACCCCCA AGTATGGCCT GGTGGGCAGG 
AGAGACTTGC TCATCTTCGA CCTGTCAGAG GGAGACAGCG GGGTGTACCA GTGCCTGTCG 
GAGGAGAGAG TCAGGAACAG GACGGTCCTG CAGCTGCTGG CCAAGCACGT TCTGGAAGTC 
AGGGTGGTTC CTAGGACCCC GGCTGCCTCC ACCCCACCAG CCGCCCGGAC GGAAGGCGAC 
AGGACCATCC TCAGAGTGTC GACGGAGCCC ACCCCAGGCC CCCCGCCCCA GACCCCGGCC 
ATGCGGGGCC CCACGCCCAG CCCCGCGCCC ACCGGCGGCA CCTGCAAACC AAAGACCATC 
ATCAACACGG TCCCTGAGGT CCACTCGGAG AAGACCATGT ATCTCAAGTC CAGTGACAAC 
CGCCTGCTCA TGTTCCTCTT CCTCTTCTTC TTCGTGCTCT GCCTCGGCCT CTTTTCCTAC 
AACTGCTACA AGGGCTACCT GCCCGGCCAC TGCCTGAAGC TCCGCTCCGC CATGCTGCTG 
GGGAAGAAGC AGCCCGCGTC CGACTTCTCC GACTTTGAGC AGAGCGTGAA GGAGACGCTG 
GTGGAGCAGG GCAGCTTCTC GCAGCAGAAC GGGGGGCAGC CCCGGCCGGC CCTGGACACC 
GGCTACGAGA CGGAGCAGGA CACCATGGCC AGCCGGGTGC CCACCGACAG GGAAGACTCG 
CAGAGGCTCG ACGACCTGCC GGTCCGGGAC AGGCCGTTTG ACGTCAAGTG TGAGCTCAAG 
TATGCCGACT CGGACGCGGA CGTGGACGCG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533551.4
CDS	107..3022
Translation	MWTFPVLSLGWRAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPNETKSFREHFFKHGGTAELKCSQKSNLARVVWTFRDRVLKAQTPKYGLVGRRDLLIFDLSEGDSGVYQCLSEERVRNRTVLQLLAKHVLEVRVVPRTPAASTPPAARTEGDRTILRVSTEPTPGPPPQTPAMRGPTPSPAPTGGTCKPKTIINTVPEVHSEKTMYLKSSDNRLLMFLFLFFFVLCLGLFSYNCYKGYLPGHCLKLRSAMLLGKKQPASDFSDFEQSVKETLVEQGSFSQQNGGQPRPALDTGYETEQDTMASRVPTDREDSQRLDDLPVRDRPFDVKCELKYADSDADVDAE

Target ORF information:

RefSeq Version	XM_533551.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533551.5

ORF Insert Sequence:

ATGTGGACTT TCCCAGTCTT GTCTCTTGGG TGGAGAGCCC AGGCTTCTGA GGACACCGTC 
CTGGGAACAA CAGGGGGCAG GTCCTGGAAG GAGAAGATTC AGAATTTCTT CGTGAGGCCG 
CAGAGAAGCG CACCATTGAA GAACCGGGTG GCCCTCGACA TCTGCGGTGG GGACCTCACC 
CAGTGCCTGG GAGATGCGGG TCCTCAGCAT TCACACCATC CACCCGCTCC GGGCCGTGCT 
GCAGGTCGTG GCCCCCCGGC TCTGAGCAGC ATTGGCCGTC ACCCCCACCA CGGGCTCCCT 
GCCCACGAGC CTTCACTACC GGAGCCCCCC CCGTGGCGCC TGCGCGTGAG AATGTGCGCC 
CCCATCGGTG GGCTGCTTGC CGCCCTGGCC ATGGCATTTG GGATGACGGT GGCATTCGCA 
CCGGTCCCCA GGATCACCTG GGAGCACAGA GAGGTGCAGC TGGAGAAGTT TCACGAGCCC 
GGCATCTTCA ACTACTCGGT GTTGCTGCTA AGTGAGGACA AGAGCACCCT GTACGTGGGT 
GCCCGGGAGG CCGTGTTTGC CCTGAGCGCA CTCAACGTCT CCAAGAAGCA GCATGAGGCG 
TATTGGAAGG TCTCGGAAGA TAAAAAAGCA AAATGTGCAG AAAAGGGCAA ATCAAGACAG 
ACAGAATGTC TCAACTACAT CCGCGTGCTG CAGCCACTCA GTGCCAGCGC CCTGTACGTG 
TGCGGGACCA ACGCGTTCCA GCCAGCCTGC GACCACCTGA ACTTAACGTC CTTTCAGTTT 
CTGGGGCGAA CAGAAGATGG AAAGGGCAGG TGCCCCTTCG ACCCCGCACA GAGCTACACT 
TCTGTCATGG TTGACGGGGA GCTGTATTCT GGGACGTCCT ACAACTTTCT GGGAAGTGAG 
CCCATCATCT CCCGAAACTC GTCCCATAGC CCTCTGAGGA CGGAGTACGC GATCCCGTGG 
CTCAACGAGC CAAGCTTCGT CTTTGCTGAC GTGATCCGAG AACACCCAGA TGGCGTGGAC 
AGCGGGGATG ACCGGGTCTA CTTCTTCTTC ACCGAGGTGT CTGTGGAGTA TGAGTTTGTG 
TTCAAGCTGA TGATCCCACG AGTGGCCAGG GTGTGCAAGG GGGACCAGGG TGGCCTGCGG 
ACCCTGCAGA AGAAGTGGAC GTCTTTCCTC AAGGCCAGGC TGATCTGTTC CCGGCCAGAC 
AACAACCTCG TCTTCAACGT GCTGCAGGAC ATCTTTGTCC TCAGGTCCCC AGACCTCAAG 
GAGCCCGTGT TCTACGGGGT CTTCACCCCA CAGCTGAACA ACGTGGGGCT GTCGGCTGTG 
TGTGCCTACA ACCTGTCCAC GGCCGAGGCG GTCTTCTCCG GCGGGAAGTA CATGCAGAGC 
GCCACGGTGG AGCAGTCCCA CACCAAGTGG GTGCGCTACA ACGGGGCCGT GCCCACGCCG 
CGGCCCGGGG CGTGCATCAA CCGTGAAGCG CGGGCCGCCA ACTTCTCCAG CTCCCTCACT 
CTGCCGGACA AGACCTTACA GTTCATCAAA GACCACCCTC TCATGGACGG CTCCGTGACC 
CCGATAGACA ACAGGCCCAG GCTGATCAAG AGAGACGTGA ACTACACGCA GATCGTGGTG 
GACAGGACAC GGGCCCTGGA CGGCACCGTC TATGACGTCA TGTTTGTCAG CACAGACCGG 
GGTGCCCTGC ACAAAGCCGT CAGCTTCGAG CATGAGGCCC ACATCATCGA GGAGACGCAG 
CTCTTCCCGG ACTTTGAGCC GATCCAGACG CTGCTGCTGA ATTCCCAGCA GGGCAAGAGA 
TTTGTGTACG CGGGTTCCAG CTCGGGTGTG GTGCAGGCCC CCGTGGCCTT CTGCGGGAAG 
CACGGGAGCT GCGAGGACTG CGTGCTGGCC CGCGACCCCT ACTGCGCCTG GAGCCGTGCC 
GCCGAGGCCT GCGTGGACCT GCACCGGACA GACAGCCCCA GCAGGACTTT GGTTCAGCAG 
ATGAGCGGTG ACACGTCCGT CTGCCCCAAT GAAACTAAAA GTTTCCGGGA GCATTTTTTC 
AAGCACGGCG GCACAGCAGA ACTGAAATGC TCCCAAAAGT CGAACCTGGC CCGGGTGGTG 
TGGACGTTCC GGGACCGCGT ACTCAAGGCC CAGACCCCCA AGTATGGCCT GGTGGGCAGG 
AGAGACTTGC TCATCTTCGA CCTGTCAGAG GGAGACAGCG GGGTGTACCA GTGCCTGTCG 
GAGGAGAGAG TCAGGAACAG GACGGTCCTG CAGCTGCTGG CCAAGCACGT TCTGGAAGTC 
AGGGTGGTTC CTAGGACCCC GGCTGCCTCC ACCCCACCAG CCGCCCGGAC GGAAGGCGAC 
AGGACCATCC TCAGAGTGTC GACGGAGCCC ACCCCAGGCC CCCCGCCCCA GACCCCGGCC 
ATGCGGGGCC CCACGCCCAG CCCCGCGCCC ACCGGCGGCA CCTGCAAACC AAAGACCATC 
ATCAACACGG TCCCTGAGGT CCACTCGGAG AAGACCATGT ATCTCAAGTC CAGTGACAAC 
CGCCTGCTCA TGTTCCTCTT CCTCTTCTTC TTCGTGCTCT GCCTCGGCCT CTTTTCCTAC 
AACTGCTACA AGGGCTACCT GCCCGGCCAC TGCCTGAAGC TCCGCTCCGC CATGCTGCTG 
GGGAAGAAGC AGCCCGCGTC CGACTTCTCC GACTTTGAGC AGAGCGTGAA GGAGACGCTG 
GTGGAGCAGG GCAGCTTCTC GCAGCAGAAC GGGGGGCAGC CCCGGCCGGC CCTGGACACC 
GGCTACGAGA CGGAGCAGGA CACCATGGCC AGCCGGGTGC CCACCGACAG GGAAGACTCG 
CAGAGGCTCG ACGACCTGCC GGTCCGGGAC AGGCCGTTTG ACGTCAAGTG TGAGCTCAAG 
TATGCCGACT CGGACGCGGA CGTGGACGCG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4D ( XM_005615959.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005616016.1 Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe01442
Clone ID Related Accession (Same CDS sequence)	XM_005615959.2
Accession Version	XM_005615959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	semaphorin-4D isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATTA CAAGCGGAGC CCAGGCTTCT GAGGACACCG TCCTGGGAAC AACAGGGGGC 
AGGTCCTGGA AGGAGAAGAT TCAGAATTTC TTCGTGAGGC CGCAGAGAAG CGCACCATTG 
AAGAACCGGG TGGCCCTCGA CATCTGCGGT GGGGACCTCA CCCAGTGCCT GGGAGATGCG 
GGTCCTCAGC ATTCACACCA TCCACCCGCT CCGGGCCGTG CTGCAGGTCG TGGCCCCCCG 
GCTCTGAGCA GCATTGGCCG TCACCCCCAC CACGGGCTCC CTGCCCACGA GCCTTCACTA 
CCGGAGCCCC CCCCGTGGCG CCTGCGCGTG AGAATGTGCG CCCCCATCGG TGGGCTGCTT 
GCCGCCCTGG CCATGGCATT TGGGATGACG GTGGCATTCG CACCGGTCCC CAGGATCACC 
TGGGAGCACA GAGAGGTGCA GCTGGAGAAG TTTCACGAGC CCGGCATCTT CAACTACTCG 
GTGTTGCTGC TAAGTGAGGA CAAGAGCACC CTGTACGTGG GTGCCCGGGA GGCCGTGTTT 
GCCCTGAGCG CACTCAACGT CTCCAAGAAG CAGCATGAGG CGTATTGGAA GGTCTCGGAA 
GATAAAAAAG CAAAATGTGC AGAAAAGGGC AAATCAAGAC AGACAGAATG TCTCAACTAC 
ATCCGCGTGC TGCAGCCACT CAGTGCCAGC GCCCTGTACG TGTGCGGGAC CAACGCGTTC 
CAGCCAGCCT GCGACCACCT GAACTTAACG TCCTTTCAGT TTCTGGGGCG AACAGAAGAT 
GGAAAGGGCA GGTGCCCCTT CGACCCCGCA CAGAGCTACA CTTCTGTCAT GGTTGACGGG 
GAGCTGTATT CTGGGACGTC CTACAACTTT CTGGGAAGTG AGCCCATCAT CTCCCGAAAC 
TCGTCCCATA GCCCTCTGAG GACGGAGTAC GCGATCCCGT GGCTCAACGA GCCAAGCTTC 
GTCTTTGCTG ACGTGATCCG AGAACACCCA GATGGCGTGG ACAGCGGGGA TGACCGGGTC 
TACTTCTTCT TCACCGAGGT GTCTGTGGAG TATGAGTTTG TGTTCAAGCT GATGATCCCA 
CGAGTGGCCA GGGTGTGCAA GGGGGACCAG GGTGGCCTGC GGACCCTGCA GAAGAAGTGG 
ACGTCTTTCC TCAAGGCCAG GCTGATCTGT TCCCGGCCAG ACAACAACCT CGTCTTCAAC 
GTGCTGCAGG ACATCTTTGT CCTCAGGTCC CCAGACCTCA AGGAGCCCGT GTTCTACGGG 
GTCTTCACCC CACAGCTGAA CAACGTGGGG CTGTCGGCTG TGTGTGCCTA CAACCTGTCC 
ACGGCCGAGG CGGTCTTCTC CGGCGGGAAG TACATGCAGA GCGCCACGGT GGAGCAGTCC 
CACACCAAGT GGGTGCGCTA CAACGGGGCC GTGCCCACGC CGCGGCCCGG GGCGTGCATC 
AACCGTGAAG CGCGGGCCGC CAACTTCTCC AGCTCCCTCA CTCTGCCGGA CAAGACCTTA 
CAGTTCATCA AAGACCACCC TCTCATGGAC GGCTCCGTGA CCCCGATAGA CAACAGGCCC 
AGGCTGATCA AGAGAGACGT GAACTACACG CAGATCGTGG TGGACAGGAC ACGGGCCCTG 
GACGGCACCG TCTATGACGT CATGTTTGTC AGCACAGACC GGGGTGCCCT GCACAAAGCC 
GTCAGCTTCG AGCATGAGGC CCACATCATC GAGGAGACGC AGCTCTTCCC GGACTTTGAG 
CCGATCCAGA CGCTGCTGCT GAATTCCCAG CAGGGCAAGA GATTTGTGTA CGCGGGTTCC 
AGCTCGGGTG TGGTGCAGGC CCCCGTGGCC TTCTGCGGGA AGCACGGGAG CTGCGAGGAC 
TGCGTGCTGG CCCGCGACCC CTACTGCGCC TGGAGCCGTG CCGCCGAGGC CTGCGTGGAC 
CTGCACCGGA CAGACAGCCC CAGCAGGACT TTGGTTCAGC AGATGAGCGG TGACACGTCC 
GTCTGCCCCA ATGAAACTAA AAGTTTCCGG GAGCATTTTT TCAAGCACGG CGGCACAGCA 
GAACTGAAAT GCTCCCAAAA GTCGAACCTG GCCCGGGTGG TGTGGACGTT CCGGGACCGC 
GTACTCAAGG CCCAGACCCC CAAGTATGGC CTGGTGGGCA GGAGAGACTT GCTCATCTTC 
GACCTGTCAG AGGGAGACAG CGGGGTGTAC CAGTGCCTGT CGGAGGAGAG AGTCAGGAAC 
AGGACGGTCC TGCAGCTGCT GGCCAAGCAC GTTCTGGAAG TCAGGGTGGT TCCTAGGACC 
CCGGCTGCCT CCACCCCACC AGCCGCCCGG ACGGAAGGCG ACAGGACCAT CCTCAGAGTG 
TCGACGGAGC CCACCCCAGG CCCCCCGCCC CAGACCCCGG CCATGCGGGG CCCCACGCCC 
AGCCCCGCGC CCACCGGCGG CACCTGCAAA CCAAAGACCA TCATCAACAC GGTCCCTGAG 
GTCCACTCGG AGAAGACCAT GTATCTCAAG TCCAGTGACA ACCGCCTGCT CATGTTCCTC 
TTCCTCTTCT TCTTCGTGCT CTGCCTCGGC CTCTTTTCCT ACAACTGCTA CAAGGGCTAC 
CTGCCCGGCC ACTGCCTGAA GCTCCGCTCC GCCATGCTGC TGGGGAAGAA GCAGCCCGCG 
TCCGACTTCT CCGACTTTGA GCAGAGCGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC 
TCGCAGCAGA ACGGGGGGCA GCCCCGGCCG GCCCTGGACA CCGGCTACGA GACGGAGCAG 
GACACCATGG CCAGCCGGGT GCCCACCGAC AGGGAAGACT CGCAGAGGCT CGACGACCTG 
CCGGTCCGGG ACAGGCCGTT TGACGTCAAG TGTGAGCTCA AGTATGCCGA CTCGGACGCG 
GACGTGGACG CGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616016.1
CDS	16..2913
Translation	MCITSGAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPNETKSFREHFFKHGGTAELKCSQKSNLARVVWTFRDRVLKAQTPKYGLVGRRDLLIFDLSEGDSGVYQCLSEERVRNRTVLQLLAKHVLEVRVVPRTPAASTPPAARTEGDRTILRVSTEPTPGPPPQTPAMRGPTPSPAPTGGTCKPKTIINTVPEVHSEKTMYLKSSDNRLLMFLFLFFFVLCLGLFSYNCYKGYLPGHCLKLRSAMLLGKKQPASDFSDFEQSVKETLVEQGSFSQQNGGQPRPALDTGYETEQDTMASRVPTDREDSQRLDDLPVRDRPFDVKCELKYADSDADVDAE

Target ORF information:

RefSeq Version	XM_005615959.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615959.2

ORF Insert Sequence:

ATGTGCATTA CAAGCGGAGC CCAGGCTTCT GAGGACACCG TCCTGGGAAC AACAGGGGGC 
AGGTCCTGGA AGGAGAAGAT TCAGAATTTC TTCGTGAGGC CGCAGAGAAG CGCACCATTG 
AAGAACCGGG TGGCCCTCGA CATCTGCGGT GGGGACCTCA CCCAGTGCCT GGGAGATGCG 
GGTCCTCAGC ATTCACACCA TCCACCCGCT CCGGGCCGTG CTGCAGGTCG TGGCCCCCCG 
GCTCTGAGCA GCATTGGCCG TCACCCCCAC CACGGGCTCC CTGCCCACGA GCCTTCACTA 
CCGGAGCCCC CCCCGTGGCG CCTGCGCGTG AGAATGTGCG CCCCCATCGG TGGGCTGCTT 
GCCGCCCTGG CCATGGCATT TGGGATGACG GTGGCATTCG CACCGGTCCC CAGGATCACC 
TGGGAGCACA GAGAGGTGCA GCTGGAGAAG TTTCACGAGC CCGGCATCTT CAACTACTCG 
GTGTTGCTGC TAAGTGAGGA CAAGAGCACC CTGTACGTGG GTGCCCGGGA GGCCGTGTTT 
GCCCTGAGCG CACTCAACGT CTCCAAGAAG CAGCATGAGG CGTATTGGAA GGTCTCGGAA 
GATAAAAAAG CAAAATGTGC AGAAAAGGGC AAATCAAGAC AGACAGAATG TCTCAACTAC 
ATCCGCGTGC TGCAGCCACT CAGTGCCAGC GCCCTGTACG TGTGCGGGAC CAACGCGTTC 
CAGCCAGCCT GCGACCACCT GAACTTAACG TCCTTTCAGT TTCTGGGGCG AACAGAAGAT 
GGAAAGGGCA GGTGCCCCTT CGACCCCGCA CAGAGCTACA CTTCTGTCAT GGTTGACGGG 
GAGCTGTATT CTGGGACGTC CTACAACTTT CTGGGAAGTG AGCCCATCAT CTCCCGAAAC 
TCGTCCCATA GCCCTCTGAG GACGGAGTAC GCGATCCCGT GGCTCAACGA GCCAAGCTTC 
GTCTTTGCTG ACGTGATCCG AGAACACCCA GATGGCGTGG ACAGCGGGGA TGACCGGGTC 
TACTTCTTCT TCACCGAGGT GTCTGTGGAG TATGAGTTTG TGTTCAAGCT GATGATCCCA 
CGAGTGGCCA GGGTGTGCAA GGGGGACCAG GGTGGCCTGC GGACCCTGCA GAAGAAGTGG 
ACGTCTTTCC TCAAGGCCAG GCTGATCTGT TCCCGGCCAG ACAACAACCT CGTCTTCAAC 
GTGCTGCAGG ACATCTTTGT CCTCAGGTCC CCAGACCTCA AGGAGCCCGT GTTCTACGGG 
GTCTTCACCC CACAGCTGAA CAACGTGGGG CTGTCGGCTG TGTGTGCCTA CAACCTGTCC 
ACGGCCGAGG CGGTCTTCTC CGGCGGGAAG TACATGCAGA GCGCCACGGT GGAGCAGTCC 
CACACCAAGT GGGTGCGCTA CAACGGGGCC GTGCCCACGC CGCGGCCCGG GGCGTGCATC 
AACCGTGAAG CGCGGGCCGC CAACTTCTCC AGCTCCCTCA CTCTGCCGGA CAAGACCTTA 
CAGTTCATCA AAGACCACCC TCTCATGGAC GGCTCCGTGA CCCCGATAGA CAACAGGCCC 
AGGCTGATCA AGAGAGACGT GAACTACACG CAGATCGTGG TGGACAGGAC ACGGGCCCTG 
GACGGCACCG TCTATGACGT CATGTTTGTC AGCACAGACC GGGGTGCCCT GCACAAAGCC 
GTCAGCTTCG AGCATGAGGC CCACATCATC GAGGAGACGC AGCTCTTCCC GGACTTTGAG 
CCGATCCAGA CGCTGCTGCT GAATTCCCAG CAGGGCAAGA GATTTGTGTA CGCGGGTTCC 
AGCTCGGGTG TGGTGCAGGC CCCCGTGGCC TTCTGCGGGA AGCACGGGAG CTGCGAGGAC 
TGCGTGCTGG CCCGCGACCC CTACTGCGCC TGGAGCCGTG CCGCCGAGGC CTGCGTGGAC 
CTGCACCGGA CAGACAGCCC CAGCAGGACT TTGGTTCAGC AGATGAGCGG TGACACGTCC 
GTCTGCCCCA ATGAAACTAA AAGTTTCCGG GAGCATTTTT TCAAGCACGG CGGCACAGCA 
GAACTGAAAT GCTCCCAAAA GTCGAACCTG GCCCGGGTGG TGTGGACGTT CCGGGACCGC 
GTACTCAAGG CCCAGACCCC CAAGTATGGC CTGGTGGGCA GGAGAGACTT GCTCATCTTC 
GACCTGTCAG AGGGAGACAG CGGGGTGTAC CAGTGCCTGT CGGAGGAGAG AGTCAGGAAC 
AGGACGGTCC TGCAGCTGCT GGCCAAGCAC GTTCTGGAAG TCAGGGTGGT TCCTAGGACC 
CCGGCTGCCT CCACCCCACC AGCCGCCCGG ACGGAAGGCG ACAGGACCAT CCTCAGAGTG 
TCGACGGAGC CCACCCCAGG CCCCCCGCCC CAGACCCCGG CCATGCGGGG CCCCACGCCC 
AGCCCCGCGC CCACCGGCGG CACCTGCAAA CCAAAGACCA TCATCAACAC GGTCCCTGAG 
GTCCACTCGG AGAAGACCAT GTATCTCAAG TCCAGTGACA ACCGCCTGCT CATGTTCCTC 
TTCCTCTTCT TCTTCGTGCT CTGCCTCGGC CTCTTTTCCT ACAACTGCTA CAAGGGCTAC 
CTGCCCGGCC ACTGCCTGAA GCTCCGCTCC GCCATGCTGC TGGGGAAGAA GCAGCCCGCG 
TCCGACTTCT CCGACTTTGA GCAGAGCGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC 
TCGCAGCAGA ACGGGGGGCA GCCCCGGCCG GCCCTGGACA CCGGCTACGA GACGGAGCAG 
GACACCATGG CCAGCCGGGT GCCCACCGAC AGGGAAGACT CGCAGAGGCT CGACGACCTG 
CCGGTCCGGG ACAGGCCGTT TGACGTCAAG TGTGAGCTCA AGTATGCCGA CTCGGACGCG 
GACGTGGACG CGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4D ( XM_014117969.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973444.1 Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X5, mRNA.

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CloneID	OCe34845
Clone ID Related Accession (Same CDS sequence)	XM_014117969.1
Accession Version	XM_014117969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	semaphorin-4D isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGAG CCCAGGCTTC TGAGGACACC GTCCTGGGAA CAACAGGGGG CAGGTCCTGG 
AAGGAGAAGA TTCAGAATTT CTTCGTGAGG CCGCAGAGAA GCGCACCATT GAAGAACCGG 
GTGGCCCTCG ACATCTGCGG TGGGGACCTC ACCCAGTGCC TGGGAGATGC GGGTCCTCAG 
CATTCACACC ATCCACCCGC TCCGGGCCGT GCTGCAGGTC GTGGCCCCCC GGCTCTGAGC 
AGCATTGGCC GTCACCCCCA CCACGGGCTC CCTGCCCACG AGCCTTCACT ACCGGAGCCC 
CCCCCGTGGC GCCTGCGCGT GAGAATGTGC GCCCCCATCG GTGGGCTGCT TGCCGCCCTG 
GCCATGGCAT TTGGGATGAC GGTGGCATTC GCACCGGTCC CCAGGATCAC CTGGGAGCAC 
AGAGAGGTGC AGCTGGAGAA GTTTCACGAG CCCGGCATCT TCAACTACTC GGTGTTGCTG 
CTAAGTGAGG ACAAGAGCAC CCTGTACGTG GGTGCCCGGG AGGCCGTGTT TGCCCTGAGC 
GCACTCAACG TCTCCAAGAA GCAGCATGAG GCGTATTGGA AGGTCTCGGA AGATAAAAAA 
GCAAAATGTG CAGAAAAGGG CAAATCAAGA CAGACAGAAT GTCTCAACTA CATCCGCGTG 
CTGCAGCCAC TCAGTGCCAG CGCCCTGTAC GTGTGCGGGA CCAACGCGTT CCAGCCAGCC 
TGCGACCACC TGAACTTAAC GTCCTTTCAG TTTCTGGGGC GAACAGAAGA TGGAAAGGGC 
AGGTGCCCCT TCGACCCCGC ACAGAGCTAC ACTTCTGTCA TGGTTGACGG GGAGCTGTAT 
TCTGGGACGT CCTACAACTT TCTGGGAAGT GAGCCCATCA TCTCCCGAAA CTCGTCCCAT 
AGCCCTCTGA GGACGGAGTA CGCGATCCCG TGGCTCAACG AGCCAAGCTT CGTCTTTGCT 
GACGTGATCC GAGAACACCC AGATGGCGTG GACAGCGGGG ATGACCGGGT CTACTTCTTC 
TTCACCGAGG TGTCTGTGGA GTATGAGTTT GTGTTCAAGC TGATGATCCC ACGAGTGGCC 
AGGGTGTGCA AGGGGGACCA GGGTGGCCTG CGGACCCTGC AGAAGAAGTG GACGTCTTTC 
CTCAAGGCCA GGCTGATCTG TTCCCGGCCA GACAACAACC TCGTCTTCAA CGTGCTGCAG 
GACATCTTTG TCCTCAGGTC CCCAGACCTC AAGGAGCCCG TGTTCTACGG GGTCTTCACC 
CCACAGCTGA ACAACGTGGG GCTGTCGGCT GTGTGTGCCT ACAACCTGTC CACGGCCGAG 
GCGGTCTTCT CCGGCGGGAA GTACATGCAG AGCGCCACGG TGGAGCAGTC CCACACCAAG 
TGGGTGCGCT ACAACGGGGC CGTGCCCACG CCGCGGCCCG GGGCGTGCAT CAACCGTGAA 
GCGCGGGCCG CCAACTTCTC CAGCTCCCTC ACTCTGCCGG ACAAGACCTT ACAGTTCATC 
AAAGACCACC CTCTCATGGA CGGCTCCGTG ACCCCGATAG ACAACAGGCC CAGGCTGATC 
AAGAGAGACG TGAACTACAC GCAGATCGTG GTGGACAGGA CACGGGCCCT GGACGGCACC 
GTCTATGACG TCATGTTTGT CAGCACAGAC CGGGGTGCCC TGCACAAAGC CGTCAGCTTC 
GAGCATGAGG CCCACATCAT CGAGGAGACG CAGCTCTTCC CGGACTTTGA GCCGATCCAG 
ACGCTGCTGC TGAATTCCCA GCAGGGCAAG AGATTTGTGT ACGCGGGTTC CAGCTCGGGT 
GTGGTGCAGG CCCCCGTGGC CTTCTGCGGG AAGCACGGGA GCTGCGAGGA CTGCGTGCTG 
GCCCGCGACC CCTACTGCGC CTGGAGCCGT GCCGCCGAGG CCTGCGTGGA CCTGCACCGG 
ACAGACAGCC CCAGCAGGAC TTTGGTTCAG CAGATGAGCG GTGACACGTC CGTCTGCCCC 
AATGAAACTA AAAGTTTCCG GGAGCATTTT TTCAAGCACG GCGGCACAGC AGAACTGAAA 
TGCTCCCAAA AGTCGAACCT GGCCCGGGTG GTGTGGACGT TCCGGGACCG CGTACTCAAG 
GCCCAGACCC CCAAGTATGG CCTGGTGGGC AGGAGAGACT TGCTCATCTT CGACCTGTCA 
GAGGGAGACA GCGGGGTGTA CCAGTGCCTG TCGGAGGAGA GAGTCAGGAA CAGGACGGTC 
CTGCAGCTGC TGGCCAAGCA CGTTCTGGAA GTCAGGGTGG TTCCTAGGAC CCCGGCTGCC 
TCCACCCCAC CAGCCGCCCG GACGGAAGGC GACAGGACCA TCCTCAGAGT GTCGACGGAG 
CCCACCCCAG GCCCCCCGCC CCAGACCCCG GCCATGCGGG GCCCCACGCC CAGCCCCGCG 
CCCACCGGCG GCACCTGCAA ACCAAAGACC ATCATCAACA CGGTCCCTGA GGTCCACTCG 
GAGAAGACCA TGTATCTCAA GTCCAGTGAC AACCGCCTGC TCATGTTCCT CTTCCTCTTC 
TTCTTCGTGC TCTGCCTCGG CCTCTTTTCC TACAACTGCT ACAAGGGCTA CCTGCCCGGC 
CACTGCCTGA AGCTCCGCTC CGCCATGCTG CTGGGGAAGA AGCAGCCCGC GTCCGACTTC 
TCCGACTTTG AGCAGAGCGT GAAGGAGACG CTGGTGGAGC AGGGCAGCTT CTCGCAGCAG 
AACGGGGGGC AGCCCCGGCC GGCCCTGGAC ACCGGCTACG AGACGGAGCA GGACACCATG 
GCCAGCCGGG TGCCCACCGA CAGGGAAGAC TCGCAGAGGC TCGACGACCT GCCGGTCCGG 
GACAGGCCGT TTGACGTCAA GTGTGAGCTC AAGTATGCCG ACTCGGACGC GGACGTGGAC 
GCGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973444.1
CDS	37..2925
Translation	MPRAQASEDTVLGTTGGRSWKEKIQNFFVRPQRSAPLKNRVALDICGGDLTQCLGDAGPQHSHHPPAPGRAAGRGPPALSSIGRHPHHGLPAHEPSLPEPPPWRLRVRMCAPIGGLLAALAMAFGMTVAFAPVPRITWEHREVQLEKFHEPGIFNYSVLLLSEDKSTLYVGAREAVFALSALNVSKKQHEAYWKVSEDKKAKCAEKGKSRQTECLNYIRVLQPLSASALYVCGTNAFQPACDHLNLTSFQFLGRTEDGKGRCPFDPAQSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIREHPDGVDSGDDRVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDNNLVFNVLQDIFVLRSPDLKEPVFYGVFTPQLNNVGLSAVCAYNLSTAEAVFSGGKYMQSATVEQSHTKWVRYNGAVPTPRPGACINREARAANFSSSLTLPDKTLQFIKDHPLMDGSVTPIDNRPRLIKRDVNYTQIVVDRTRALDGTVYDVMFVSTDRGALHKAVSFEHEAHIIEETQLFPDFEPIQTLLLNSQQGKRFVYAGSSSGVVQAPVAFCGKHGSCEDCVLARDPYCAWSRAAEACVDLHRTDSPSRTLVQQMSGDTSVCPNETKSFREHFFKHGGTAELKCSQKSNLARVVWTFRDRVLKAQTPKYGLVGRRDLLIFDLSEGDSGVYQCLSEERVRNRTVLQLLAKHVLEVRVVPRTPAASTPPAARTEGDRTILRVSTEPTPGPPPQTPAMRGPTPSPAPTGGTCKPKTIINTVPEVHSEKTMYLKSSDNRLLMFLFLFFFVLCLGLFSYNCYKGYLPGHCLKLRSAMLLGKKQPASDFSDFEQSVKETLVEQGSFSQQNGGQPRPALDTGYETEQDTMASRVPTDREDSQRLDDLPVRDRPFDVKCELKYADSDADVDAE

Target ORF information:

RefSeq Version	XM_014117969.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117969.1

ORF Insert Sequence:

ATGCCGAGAG CCCAGGCTTC TGAGGACACC GTCCTGGGAA CAACAGGGGG CAGGTCCTGG 
AAGGAGAAGA TTCAGAATTT CTTCGTGAGG CCGCAGAGAA GCGCACCATT GAAGAACCGG 
GTGGCCCTCG ACATCTGCGG TGGGGACCTC ACCCAGTGCC TGGGAGATGC GGGTCCTCAG 
CATTCACACC ATCCACCCGC TCCGGGCCGT GCTGCAGGTC GTGGCCCCCC GGCTCTGAGC 
AGCATTGGCC GTCACCCCCA CCACGGGCTC CCTGCCCACG AGCCTTCACT ACCGGAGCCC 
CCCCCGTGGC GCCTGCGCGT GAGAATGTGC GCCCCCATCG GTGGGCTGCT TGCCGCCCTG 
GCCATGGCAT TTGGGATGAC GGTGGCATTC GCACCGGTCC CCAGGATCAC CTGGGAGCAC 
AGAGAGGTGC AGCTGGAGAA GTTTCACGAG CCCGGCATCT TCAACTACTC GGTGTTGCTG 
CTAAGTGAGG ACAAGAGCAC CCTGTACGTG GGTGCCCGGG AGGCCGTGTT TGCCCTGAGC 
GCACTCAACG TCTCCAAGAA GCAGCATGAG GCGTATTGGA AGGTCTCGGA AGATAAAAAA 
GCAAAATGTG CAGAAAAGGG CAAATCAAGA CAGACAGAAT GTCTCAACTA CATCCGCGTG 
CTGCAGCCAC TCAGTGCCAG CGCCCTGTAC GTGTGCGGGA CCAACGCGTT CCAGCCAGCC 
TGCGACCACC TGAACTTAAC GTCCTTTCAG TTTCTGGGGC GAACAGAAGA TGGAAAGGGC 
AGGTGCCCCT TCGACCCCGC ACAGAGCTAC ACTTCTGTCA TGGTTGACGG GGAGCTGTAT 
TCTGGGACGT CCTACAACTT TCTGGGAAGT GAGCCCATCA TCTCCCGAAA CTCGTCCCAT 
AGCCCTCTGA GGACGGAGTA CGCGATCCCG TGGCTCAACG AGCCAAGCTT CGTCTTTGCT 
GACGTGATCC GAGAACACCC AGATGGCGTG GACAGCGGGG ATGACCGGGT CTACTTCTTC 
TTCACCGAGG TGTCTGTGGA GTATGAGTTT GTGTTCAAGC TGATGATCCC ACGAGTGGCC 
AGGGTGTGCA AGGGGGACCA GGGTGGCCTG CGGACCCTGC AGAAGAAGTG GACGTCTTTC 
CTCAAGGCCA GGCTGATCTG TTCCCGGCCA GACAACAACC TCGTCTTCAA CGTGCTGCAG 
GACATCTTTG TCCTCAGGTC CCCAGACCTC AAGGAGCCCG TGTTCTACGG GGTCTTCACC 
CCACAGCTGA ACAACGTGGG GCTGTCGGCT GTGTGTGCCT ACAACCTGTC CACGGCCGAG 
GCGGTCTTCT CCGGCGGGAA GTACATGCAG AGCGCCACGG TGGAGCAGTC CCACACCAAG 
TGGGTGCGCT ACAACGGGGC CGTGCCCACG CCGCGGCCCG GGGCGTGCAT CAACCGTGAA 
GCGCGGGCCG CCAACTTCTC CAGCTCCCTC ACTCTGCCGG ACAAGACCTT ACAGTTCATC 
AAAGACCACC CTCTCATGGA CGGCTCCGTG ACCCCGATAG ACAACAGGCC CAGGCTGATC 
AAGAGAGACG TGAACTACAC GCAGATCGTG GTGGACAGGA CACGGGCCCT GGACGGCACC 
GTCTATGACG TCATGTTTGT CAGCACAGAC CGGGGTGCCC TGCACAAAGC CGTCAGCTTC 
GAGCATGAGG CCCACATCAT CGAGGAGACG CAGCTCTTCC CGGACTTTGA GCCGATCCAG 
ACGCTGCTGC TGAATTCCCA GCAGGGCAAG AGATTTGTGT ACGCGGGTTC CAGCTCGGGT 
GTGGTGCAGG CCCCCGTGGC CTTCTGCGGG AAGCACGGGA GCTGCGAGGA CTGCGTGCTG 
GCCCGCGACC CCTACTGCGC CTGGAGCCGT GCCGCCGAGG CCTGCGTGGA CCTGCACCGG 
ACAGACAGCC CCAGCAGGAC TTTGGTTCAG CAGATGAGCG GTGACACGTC CGTCTGCCCC 
AATGAAACTA AAAGTTTCCG GGAGCATTTT TTCAAGCACG GCGGCACAGC AGAACTGAAA 
TGCTCCCAAA AGTCGAACCT GGCCCGGGTG GTGTGGACGT TCCGGGACCG CGTACTCAAG 
GCCCAGACCC CCAAGTATGG CCTGGTGGGC AGGAGAGACT TGCTCATCTT CGACCTGTCA 
GAGGGAGACA GCGGGGTGTA CCAGTGCCTG TCGGAGGAGA GAGTCAGGAA CAGGACGGTC 
CTGCAGCTGC TGGCCAAGCA CGTTCTGGAA GTCAGGGTGG TTCCTAGGAC CCCGGCTGCC 
TCCACCCCAC CAGCCGCCCG GACGGAAGGC GACAGGACCA TCCTCAGAGT GTCGACGGAG 
CCCACCCCAG GCCCCCCGCC CCAGACCCCG GCCATGCGGG GCCCCACGCC CAGCCCCGCG 
CCCACCGGCG GCACCTGCAA ACCAAAGACC ATCATCAACA CGGTCCCTGA GGTCCACTCG 
GAGAAGACCA TGTATCTCAA GTCCAGTGAC AACCGCCTGC TCATGTTCCT CTTCCTCTTC 
TTCTTCGTGC TCTGCCTCGG CCTCTTTTCC TACAACTGCT ACAAGGGCTA CCTGCCCGGC 
CACTGCCTGA AGCTCCGCTC CGCCATGCTG CTGGGGAAGA AGCAGCCCGC GTCCGACTTC 
TCCGACTTTG AGCAGAGCGT GAAGGAGACG CTGGTGGAGC AGGGCAGCTT CTCGCAGCAG 
AACGGGGGGC AGCCCCGGCC GGCCCTGGAC ACCGGCTACG AGACGGAGCA GGACACCATG 
GCCAGCCGGG TGCCCACCGA CAGGGAAGAC TCGCAGAGGC TCGACGACCT GCCGGTCCGG 
GACAGGCCGT TTGACGTCAA GTGTGAGCTC AAGTATGCCG ACTCGGACGC GGACGTGGAC 
GCGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SEMA4D cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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