MAP3K4 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014113049.2	XP_013968524.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_022418662.1	XP_022274370.1	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_014113044.1	XP_013968519.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_014113046.2	XP_013968521.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_014113046.1	XP_013968521.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_014113049.1	XP_013968524.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_014113044.2	XP_013968519.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_022418666.1	XP_022274374.1	mitogen-activated protein kinase kinase kinase 4 isoform X6
XM_022418664.1	XP_022274372.1	mitogen-activated protein kinase kinase kinase 4 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe285901	XM_022418664.1 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe285900	XM_022418662.1 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe285902	XM_022418666.1 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe34474	XM_014113044.2 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe34475	XM_014113046.2 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe34476	XM_014113049.2 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OCe34475	XM_014113046.1	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe34476	XM_014113049.1	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe34474	XM_014113044.1	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

MAP3K4 ( XM_022418664.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274372.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe285901
Clone ID Related Accession (Same CDS sequence)	XM_022418664.1
Accession Version	XM_022418664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGCA TGTCCACCAA ACACCAGAGG AATAACGTGG GGAAGCCTGC CAATCGATCT 
GGTTTGAAAG AAAAAATGAA TGCACCAAAC CAGCCTCCAC ACAAAGACAC TGGAAAAACC 
ATGGAGAATG TGGAAGAATA TAGCTATAAG CAGGAGAAGA AGATCCGAGC TGCTCTTAGG 
ACAACAGAGC GTGATCATAA GAAAAATGTG CAGTGCTCGT TCATGTTGGA CTCAGTGGGT 
GGGTCTTTGC CCAAAAAATC AATTCCAGAT GTGGATCTTA ATAAGCCTTA CCTCAGTCTT 
GGCTGCAGCA ATGCTAAGCT TCCGGTATCT GTGCCCATGC CTATACCCAG AACTGCACGC 
CAGACTTCTA GGACTGACTG TCCAGCAGAT CGCTTAAAGT TTTTTGAGAC TTTACGACTT 
CTGCTAAAGC TTACCTCAGT CTCAAAGAAG AAGGACAGGG AGACAGGAGA AACGAAAAAT 
ACATCTGCTT TCTGGTTTAA CCGATCTAAT GAACTGATCT GGTTAGAGCT ACAAGCCTGG 
CACGCAGGAC GGACCATTAA TGACCAGGAC CTCTTTTTAT ATACAGCCCG TCAAGCCATC 
CCTGATATTA TTAATGAAAT CCTTACTTTC AAAGTTAATT ATGGGAGCTT TGCCTTTGTT 
AGAAATGGAG CTAGTTTTAA TGGTACTTCA GTAGAAGGGC AGTGCAGAGC CCCTCATGGA 
ACAAAAATTG TGTGTTACTC AACATATCAT GAGCATCTCC AACGCCAGAG AGTGTCATTT 
GAGCAGGTAA AGCGGATAAT GGAGCTGCTG GAGTACATGG AAGCACTTTA TCCATCGTTG 
CAGGCTCTTC AAAAGGACTA TGAAAAATAT GCTGCAAAAG ACTTCCAGGA CAGGGTGCAG 
GCACTCTGTT TGTGGTTAAA CATCACAAAG GACTTAAATC AGAAGTTAAG GATTATGGGC 
ACTGTTTTGG GCATCAAGAA TTTATCAGAC ATTGGCTGGC CAGTGTTTGA AATCCCTTCT 
CCTCGATCGT CCAAAGGCAA TGAGCCAGAA GATGAGGGTG ATGACACAGA AGGGGACTTG 
AAAGAGTTGG ATAGTAGTAC GGATGAGAGT GAAGAAGAAC AACTCTCAGG TCCAAGGGCA 
CCAGAGCCCA CACAGCCCAT TGACACCAAT TTCAGCATCC ATTCACAGGA TTGTGTACTG 
AAGAAGCTTG AGAGGCTTGA GTCTGAGGAT GATTCTTTTG GCTGGGGAGC ACCAGACTGC 
AGCACAGAGG CAGGCTTTAG TAGACATTGT CTGACTTCTA TTTATAGGCC ATTTGTAGAC 
AAAGCCCTGA AGCAAATGGG GTTAAGAAAG TTAATTTTAA GACTTCACAA GCTGATGGAC 
GGTTCCTTGC AAAGGGCACG TATAGCACTG GTAAAGAGTG ACCATCCAGT GGAGTTTTCC 
GAATTTCCAG ACCCCATGTG GGGTTCCGAT TATGTGCAGT TATCCAGGAC ACCACCTTCC 
TCGGAGCAGA AGGGCAGCAC TGTGTCGTGG GACGAGCTAA AGTCCATGGA CTTACCCTCC 
TTCGAACCTG CCTTCCTGGT GCTCTGTCGA GTCCTTCTGA ATGTGATACA TGAGTGTCTG 
AAGTTAAGAC TGGAGCAGAG GCCTGCTGGA GAACCATCTC TCTTAAGTAT TAAGCAGCTG 
GTGAGGGAGT GTAAAGAGGT GCTAAAGGGC GGCCTTCTGA TGAAGCAGTA CTACCAGTTC 
ATGCTGCACG AGGTGCTGGC CGACCTGCAG AAGACTGACT GCAACATCGA CGCCTTTGAG 
GAGGACCTGC ACAAAATGCT GATGGTGTAT TTTGATTACA TGAGAAGCTG GATCCAAATG 
CTGCAGCAGT TACCTCAAGC ATCCCATAGT CTAAAAAACC TGTTAGAAGA AGAGTGGAAT 
TTCACCAAAG AAATAACCCA TTACATCCGG GGAGGAGAAG CCCAGGCTGG AAAACTTTTC 
TGTGACATTG CAGGGATGCT GCTGAAGTCA ACAGGGAGTT TTTTAGAGTT CGGCTTGCAG 
GAGAGCTGTG CAGAGTTCTG GACCAGCGCT GACGACTCCA ATGCCTCAGA CGAAATAAGG 
AGATCCGTTA TAGAGATCAG TCGAGCTCTG AAGGAGCTTT TTCACGAAGC TAGAGAAAGA 
GCCTCCAAGG CGCTTGGATT TGCTAAAATG TTGAGAAAGG ACCTGGAAAT AGCAGCAGAG 
TTCATACTTT CAGCCCCCAT TAGAGACCTT CTGGATGTTC TGAAATCAAA ACAGTATGTT 
AAGGTACAGA TTCCTGGCTT AGAAAACTTG CAAGTGTTTG TTCCAGACAC TCTTGCTGAG 
GAGAAGAACA TTATTTTGCA GTTACTCAAT GCAGCCGCAG GAAAAGATTG CTCGAAAGAG 
TCAGATGATG TACTGATTGA TGCCTATCTG TTGCTAACCA AGCAGAGTGA TCGCGCTCGG 
GATTCAGAGG ATAGTTGGGC CTCATGGGAA GTGCGACCTG TCAAAATTGT GCCTCAGGTC 
GAGACCGTCG ACACCCTGAG AAGCATGCAG GTGGATAACC TTTTACTAGT TGTCATGCAG 
TCTGCACATC TCACCATCCA GAGAAAAGCT TTCCAGCAGT CCATCGAGGG ACTCATGACT 
CTCCGCCAGG AGCAGACGTC CAGTCAGCCG GTCATCGCCA GAGCTTTACA GCAGTTAAAA 
AATGATGCAC TAGAGCTTTG CAACAGAATA AGCGATGCCA TTGACCGAGT GGACCACATG 
TTCACTTCAG AATTTGATGC GGAAGTTGAT GAATCAGAGT CTGTCACATT GCAACAGTAC 
TACCGAGAGG CAATGATTCA GGGCTACAAC TTTGGGTTCG AGTATCATAA AGAAGTTGTT 
CGTTTGATGT CTGGAGAATT TAGACAGAAG ATAGGAGACA AATATATAAG CTTTGCCCGG 
AAATGGATGA ACTATGTCCT AACTAAATGT GAGAGTGGTC GAGGTACAAG ACCCAGGTGG 
GCAACCCAAG GTTTTGACTT TCTACAAGCC ATTGAACCTG CCTTTATTTC AGCTTTGCCA 
GAAGATGACT TCTTGAGTCT TCAAGCCTTG ATGAATGAGT GCATTGGCCA TGTGATAGGA 
AAGCCACACA GTCCTGTTAC AGGTTTGTAC CTTGCTATTC ATCGGAACAG TCCCCGCCCT 
GTGAAGGTAC CACGATGCCA TAGTGACCCT CCCAACCCAC ATCTAATTAT CCCGACTCCA 
GAGGGATTCA GCACTCGGAG CGTGCCTTCA GACGCACGGA GCCACGGCAG CCCTGCTGCT 
GCTCCTGTCC CTGCTGCCGC CGCCACCGCC GGTCGCCCCG GCCCCGCAGG CAGCGACTCT 
GCACCACCCA AACCCATCAG CAGTGCCCAT GATACCAGGG GTTCCAGTGT CCCTGAGAAT 
GACCGGTTGG CTTCTATAGC CGCCGAGTTA CAGTTTCGGT CCTTGAGCCG CCACTCAAGC 
CCCACGGAGG AGCGCGACGA ACCAGCGTAT CCAAAAGGTG ATTCAAGTGG GTCAGCTCGA 
AGAAGTTGGG AACTTCGGAC ACTTATCAGC CAGACTAAGG ACAGTGCTTC CAAACAAGGA 
CCCATAGAAG CTATCCAGAA ATCAGTCCGA CTGTTTGAAG AAAAGAGGTA TCGAGAAATG 
AGGAGAAAGA ATATCATTGG TCAAGTTTGT GATACCCCTA AATCATATGA TAACGTTATG 
CATGTAGGTT TGAGGAAGGT GACCTTCAAG TGGCAAAGAG GAAACAAAAT TGGGGAGGGG 
CAGTATGGGA AGGTCTACAC CTGTATCAGT GTTGACACTG GCGAATTGAT GGCTATGAAG 
GAGATCCGAT TTCAACCTAA TGACCATAAA ACTATCAAGG AAACTGCAGA CGAATTGAAA 
ATCTTTGAGG GCATCAAACA CCCCAATCTG GTTCGGTATT TTGGTGTGGA GCTTCATAGA 
GAAGAAATGT ACATCTTCAT GGAATACTGT GACGAGGGGA CATTAGAAGA GGTGTCAAGG 
CTGGGACTTC AGGAGCATGT CATTAGGCTG TATTCAAAGC AGATCACCAT CGCAATCAAC 
GTCCTCCATG AGCATGGCAT CGTTCACCGT GACATCAAAG GTGCCAATAT CTTCCTAACC 
TCCTCTGGAC TCATCAAGCT GGGGGATTTT GGATGCTCAG TAAAGCTTAA AAACAACGCA 
CAGACCATGC CGGGAGAAGT GAATAGCACA CTGGGGACAG CAGCATACAT GGCTCCAGAA 
GTCATCACTC GTGCAAAAGG AGAAGGCCAT GGGCGTGCCG CTGATATCTG GAGTCTGGGC 
TGTGTCGTCA TAGAGATGGT GACCGGCAAG AGGCCTTGGC ATGAATATGA ACACAACTTT 
CAGATTATGT ATAAAGTGGG AATGGGGCAC AAGCCACCCA TCCCTGAGAG ACTAAGCCCG 
GAAGGAAAGG ACTTCCTTTC TCACTGCCTT GAAAGTGAGC CCAGGATGAG GTGGACGGCC 
AGCCAGCTCC TCGACCATTC CTTCGTCAAG GTCTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274372.1
CDS	137..4687
Translation	MKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAPVPAAAATAGRPGPAGSDSAPPKPISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_022418664.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418664.1

ORF Insert Sequence:

ATGAAACGCA TGTCCACCAA ACACCAGAGG AATAACGTGG GGAAGCCTGC CAATCGATCT 
GGTTTGAAAG AAAAAATGAA TGCACCAAAC CAGCCTCCAC ACAAAGACAC TGGAAAAACC 
ATGGAGAATG TGGAAGAATA TAGCTATAAG CAGGAGAAGA AGATCCGAGC TGCTCTTAGG 
ACAACAGAGC GTGATCATAA GAAAAATGTG CAGTGCTCGT TCATGTTGGA CTCAGTGGGT 
GGGTCTTTGC CCAAAAAATC AATTCCAGAT GTGGATCTTA ATAAGCCTTA CCTCAGTCTT 
GGCTGCAGCA ATGCTAAGCT TCCGGTATCT GTGCCCATGC CTATACCCAG AACTGCACGC 
CAGACTTCTA GGACTGACTG TCCAGCAGAT CGCTTAAAGT TTTTTGAGAC TTTACGACTT 
CTGCTAAAGC TTACCTCAGT CTCAAAGAAG AAGGACAGGG AGACAGGAGA AACGAAAAAT 
ACATCTGCTT TCTGGTTTAA CCGATCTAAT GAACTGATCT GGTTAGAGCT ACAAGCCTGG 
CACGCAGGAC GGACCATTAA TGACCAGGAC CTCTTTTTAT ATACAGCCCG TCAAGCCATC 
CCTGATATTA TTAATGAAAT CCTTACTTTC AAAGTTAATT ATGGGAGCTT TGCCTTTGTT 
AGAAATGGAG CTAGTTTTAA TGGTACTTCA GTAGAAGGGC AGTGCAGAGC CCCTCATGGA 
ACAAAAATTG TGTGTTACTC AACATATCAT GAGCATCTCC AACGCCAGAG AGTGTCATTT 
GAGCAGGTAA AGCGGATAAT GGAGCTGCTG GAGTACATGG AAGCACTTTA TCCATCGTTG 
CAGGCTCTTC AAAAGGACTA TGAAAAATAT GCTGCAAAAG ACTTCCAGGA CAGGGTGCAG 
GCACTCTGTT TGTGGTTAAA CATCACAAAG GACTTAAATC AGAAGTTAAG GATTATGGGC 
ACTGTTTTGG GCATCAAGAA TTTATCAGAC ATTGGCTGGC CAGTGTTTGA AATCCCTTCT 
CCTCGATCGT CCAAAGGCAA TGAGCCAGAA GATGAGGGTG ATGACACAGA AGGGGACTTG 
AAAGAGTTGG ATAGTAGTAC GGATGAGAGT GAAGAAGAAC AACTCTCAGG TCCAAGGGCA 
CCAGAGCCCA CACAGCCCAT TGACACCAAT TTCAGCATCC ATTCACAGGA TTGTGTACTG 
AAGAAGCTTG AGAGGCTTGA GTCTGAGGAT GATTCTTTTG GCTGGGGAGC ACCAGACTGC 
AGCACAGAGG CAGGCTTTAG TAGACATTGT CTGACTTCTA TTTATAGGCC ATTTGTAGAC 
AAAGCCCTGA AGCAAATGGG GTTAAGAAAG TTAATTTTAA GACTTCACAA GCTGATGGAC 
GGTTCCTTGC AAAGGGCACG TATAGCACTG GTAAAGAGTG ACCATCCAGT GGAGTTTTCC 
GAATTTCCAG ACCCCATGTG GGGTTCCGAT TATGTGCAGT TATCCAGGAC ACCACCTTCC 
TCGGAGCAGA AGGGCAGCAC TGTGTCGTGG GACGAGCTAA AGTCCATGGA CTTACCCTCC 
TTCGAACCTG CCTTCCTGGT GCTCTGTCGA GTCCTTCTGA ATGTGATACA TGAGTGTCTG 
AAGTTAAGAC TGGAGCAGAG GCCTGCTGGA GAACCATCTC TCTTAAGTAT TAAGCAGCTG 
GTGAGGGAGT GTAAAGAGGT GCTAAAGGGC GGCCTTCTGA TGAAGCAGTA CTACCAGTTC 
ATGCTGCACG AGGTGCTGGC CGACCTGCAG AAGACTGACT GCAACATCGA CGCCTTTGAG 
GAGGACCTGC ACAAAATGCT GATGGTGTAT TTTGATTACA TGAGAAGCTG GATCCAAATG 
CTGCAGCAGT TACCTCAAGC ATCCCATAGT CTAAAAAACC TGTTAGAAGA AGAGTGGAAT 
TTCACCAAAG AAATAACCCA TTACATCCGG GGAGGAGAAG CCCAGGCTGG AAAACTTTTC 
TGTGACATTG CAGGGATGCT GCTGAAGTCA ACAGGGAGTT TTTTAGAGTT CGGCTTGCAG 
GAGAGCTGTG CAGAGTTCTG GACCAGCGCT GACGACTCCA ATGCCTCAGA CGAAATAAGG 
AGATCCGTTA TAGAGATCAG TCGAGCTCTG AAGGAGCTTT TTCACGAAGC TAGAGAAAGA 
GCCTCCAAGG CGCTTGGATT TGCTAAAATG TTGAGAAAGG ACCTGGAAAT AGCAGCAGAG 
TTCATACTTT CAGCCCCCAT TAGAGACCTT CTGGATGTTC TGAAATCAAA ACAGTATGTT 
AAGGTACAGA TTCCTGGCTT AGAAAACTTG CAAGTGTTTG TTCCAGACAC TCTTGCTGAG 
GAGAAGAACA TTATTTTGCA GTTACTCAAT GCAGCCGCAG GAAAAGATTG CTCGAAAGAG 
TCAGATGATG TACTGATTGA TGCCTATCTG TTGCTAACCA AGCAGAGTGA TCGCGCTCGG 
GATTCAGAGG ATAGTTGGGC CTCATGGGAA GTGCGACCTG TCAAAATTGT GCCTCAGGTC 
GAGACCGTCG ACACCCTGAG AAGCATGCAG GTGGATAACC TTTTACTAGT TGTCATGCAG 
TCTGCACATC TCACCATCCA GAGAAAAGCT TTCCAGCAGT CCATCGAGGG ACTCATGACT 
CTCCGCCAGG AGCAGACGTC CAGTCAGCCG GTCATCGCCA GAGCTTTACA GCAGTTAAAA 
AATGATGCAC TAGAGCTTTG CAACAGAATA AGCGATGCCA TTGACCGAGT GGACCACATG 
TTCACTTCAG AATTTGATGC GGAAGTTGAT GAATCAGAGT CTGTCACATT GCAACAGTAC 
TACCGAGAGG CAATGATTCA GGGCTACAAC TTTGGGTTCG AGTATCATAA AGAAGTTGTT 
CGTTTGATGT CTGGAGAATT TAGACAGAAG ATAGGAGACA AATATATAAG CTTTGCCCGG 
AAATGGATGA ACTATGTCCT AACTAAATGT GAGAGTGGTC GAGGTACAAG ACCCAGGTGG 
GCAACCCAAG GTTTTGACTT TCTACAAGCC ATTGAACCTG CCTTTATTTC AGCTTTGCCA 
GAAGATGACT TCTTGAGTCT TCAAGCCTTG ATGAATGAGT GCATTGGCCA TGTGATAGGA 
AAGCCACACA GTCCTGTTAC AGGTTTGTAC CTTGCTATTC ATCGGAACAG TCCCCGCCCT 
GTGAAGGTAC CACGATGCCA TAGTGACCCT CCCAACCCAC ATCTAATTAT CCCGACTCCA 
GAGGGATTCA GCACTCGGAG CGTGCCTTCA GACGCACGGA GCCACGGCAG CCCTGCTGCT 
GCTCCTGTCC CTGCTGCCGC CGCCACCGCC GGTCGCCCCG GCCCCGCAGG CAGCGACTCT 
GCACCACCCA AACCCATCAG CAGTGCCCAT GATACCAGGG GTTCCAGTGT CCCTGAGAAT 
GACCGGTTGG CTTCTATAGC CGCCGAGTTA CAGTTTCGGT CCTTGAGCCG CCACTCAAGC 
CCCACGGAGG AGCGCGACGA ACCAGCGTAT CCAAAAGGTG ATTCAAGTGG GTCAGCTCGA 
AGAAGTTGGG AACTTCGGAC ACTTATCAGC CAGACTAAGG ACAGTGCTTC CAAACAAGGA 
CCCATAGAAG CTATCCAGAA ATCAGTCCGA CTGTTTGAAG AAAAGAGGTA TCGAGAAATG 
AGGAGAAAGA ATATCATTGG TCAAGTTTGT GATACCCCTA AATCATATGA TAACGTTATG 
CATGTAGGTT TGAGGAAGGT GACCTTCAAG TGGCAAAGAG GAAACAAAAT TGGGGAGGGG 
CAGTATGGGA AGGTCTACAC CTGTATCAGT GTTGACACTG GCGAATTGAT GGCTATGAAG 
GAGATCCGAT TTCAACCTAA TGACCATAAA ACTATCAAGG AAACTGCAGA CGAATTGAAA 
ATCTTTGAGG GCATCAAACA CCCCAATCTG GTTCGGTATT TTGGTGTGGA GCTTCATAGA 
GAAGAAATGT ACATCTTCAT GGAATACTGT GACGAGGGGA CATTAGAAGA GGTGTCAAGG 
CTGGGACTTC AGGAGCATGT CATTAGGCTG TATTCAAAGC AGATCACCAT CGCAATCAAC 
GTCCTCCATG AGCATGGCAT CGTTCACCGT GACATCAAAG GTGCCAATAT CTTCCTAACC 
TCCTCTGGAC TCATCAAGCT GGGGGATTTT GGATGCTCAG TAAAGCTTAA AAACAACGCA 
CAGACCATGC CGGGAGAAGT GAATAGCACA CTGGGGACAG CAGCATACAT GGCTCCAGAA 
GTCATCACTC GTGCAAAAGG AGAAGGCCAT GGGCGTGCCG CTGATATCTG GAGTCTGGGC 
TGTGTCGTCA TAGAGATGGT GACCGGCAAG AGGCCTTGGC ATGAATATGA ACACAACTTT 
CAGATTATGT ATAAAGTGGG AATGGGGCAC AAGCCACCCA TCCCTGAGAG ACTAAGCCCG 
GAAGGAAAGG ACTTCCTTTC TCACTGCCTT GAAAGTGAGC CCAGGATGAG GTGGACGGCC 
AGCCAGCTCC TCGACCATTC CTTCGTCAAG GTCTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_022418662.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274370.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe285900
Clone ID Related Accession (Same CDS sequence)	XM_022418662.1
Accession Version	XM_022418662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGCT 
ATTCATCGGA ACAGTCCCCG CCCTGTGAAG GTACCACGAT GCCATAGTGA CCCTCCCAAC 
CCACATCTAA TTATCCCGAC TCCAGAGGGA TTCAGGGGTT CCAGTGTCCC TGAGAATGAC 
CGGTTGGCTT CTATAGCCGC CGAGTTACAG TTTCGGTCCT TGAGCCGCCA CTCAAGCCCC 
ACGGAGGAGC GCGACGAACC AGCGTATCCA AAAGGTGATT CAAGTGGGTC AGCTCGAAGA 
AGTTGGGAAC TTCGGACACT TATCAGCCAG ACTAAGGACA GTGCTTCCAA ACAAGGACCC 
ATAGAAGCTA TCCAGAAATC AGTCCGACTG TTTGAAGAAA AGAGGTATCG AGAAATGAGG 
AGAAAGAATA TCATTGGTCA AGTTTGTGAT ACCCCTAAAT CATATGATAA CGTTATGCAT 
GTAGGTTTGA GGAAGGTGAC CTTCAAGTGG CAAAGAGGAA ACAAAATTGG GGAGGGGCAG 
TATGGGAAGG TCTACACCTG TATCAGTGTT GACACTGGCG AATTGATGGC TATGAAGGAG 
ATCCGATTTC AACCTAATGA CCATAAAACT ATCAAGGAAA CTGCAGACGA ATTGAAAATC 
TTTGAGGGCA TCAAACACCC CAATCTGGTT CGGTATTTTG GTGTGGAGCT TCATAGAGAA 
GAAATGTACA TCTTCATGGA ATACTGTGAC GAGGGGACAT TAGAAGAGGT GTCAAGGCTG 
GGACTTCAGG AGCATGTCAT TAGGCTGTAT TCAAAGCAGA TCACCATCGC AATCAACGTC 
CTCCATGAGC ATGGCATCGT TCACCGTGAC ATCAAAGGTG CCAATATCTT CCTAACCTCC 
TCTGGACTCA TCAAGCTGGG GGATTTTGGA TGCTCAGTAA AGCTTAAAAA CAACGCACAG 
ACCATGCCGG GAGAAGTGAA TAGCACACTG GGGACAGCAG CATACATGGC TCCAGAAGTC 
ATCACTCGTG CAAAAGGAGA AGGCCATGGG CGTGCCGCTG ATATCTGGAG TCTGGGCTGT 
GTCGTCATAG AGATGGTGAC CGGCAAGAGG CCTTGGCATG AATATGAACA CAACTTTCAG 
ATTATGTATA AAGTGGGAAT GGGGCACAAG CCACCCATCC CTGAGAGACT AAGCCCGGAA 
GGAAAGGACT TCCTTTCTCA CTGCCTTGAA AGTGAGCCCA GGATGAGGTG GACGGCCAGC 
CAGCTCCTCG ACCATTCCTT CGTCAAGGTC TGCACAGATG AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274370.1
CDS	187..4854
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_022418662.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418662.1

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGCT 
ATTCATCGGA ACAGTCCCCG CCCTGTGAAG GTACCACGAT GCCATAGTGA CCCTCCCAAC 
CCACATCTAA TTATCCCGAC TCCAGAGGGA TTCAGGGGTT CCAGTGTCCC TGAGAATGAC 
CGGTTGGCTT CTATAGCCGC CGAGTTACAG TTTCGGTCCT TGAGCCGCCA CTCAAGCCCC 
ACGGAGGAGC GCGACGAACC AGCGTATCCA AAAGGTGATT CAAGTGGGTC AGCTCGAAGA 
AGTTGGGAAC TTCGGACACT TATCAGCCAG ACTAAGGACA GTGCTTCCAA ACAAGGACCC 
ATAGAAGCTA TCCAGAAATC AGTCCGACTG TTTGAAGAAA AGAGGTATCG AGAAATGAGG 
AGAAAGAATA TCATTGGTCA AGTTTGTGAT ACCCCTAAAT CATATGATAA CGTTATGCAT 
GTAGGTTTGA GGAAGGTGAC CTTCAAGTGG CAAAGAGGAA ACAAAATTGG GGAGGGGCAG 
TATGGGAAGG TCTACACCTG TATCAGTGTT GACACTGGCG AATTGATGGC TATGAAGGAG 
ATCCGATTTC AACCTAATGA CCATAAAACT ATCAAGGAAA CTGCAGACGA ATTGAAAATC 
TTTGAGGGCA TCAAACACCC CAATCTGGTT CGGTATTTTG GTGTGGAGCT TCATAGAGAA 
GAAATGTACA TCTTCATGGA ATACTGTGAC GAGGGGACAT TAGAAGAGGT GTCAAGGCTG 
GGACTTCAGG AGCATGTCAT TAGGCTGTAT TCAAAGCAGA TCACCATCGC AATCAACGTC 
CTCCATGAGC ATGGCATCGT TCACCGTGAC ATCAAAGGTG CCAATATCTT CCTAACCTCC 
TCTGGACTCA TCAAGCTGGG GGATTTTGGA TGCTCAGTAA AGCTTAAAAA CAACGCACAG 
ACCATGCCGG GAGAAGTGAA TAGCACACTG GGGACAGCAG CATACATGGC TCCAGAAGTC 
ATCACTCGTG CAAAAGGAGA AGGCCATGGG CGTGCCGCTG ATATCTGGAG TCTGGGCTGT 
GTCGTCATAG AGATGGTGAC CGGCAAGAGG CCTTGGCATG AATATGAACA CAACTTTCAG 
ATTATGTATA AAGTGGGAAT GGGGCACAAG CCACCCATCC CTGAGAGACT AAGCCCGGAA 
GGAAAGGACT TCCTTTCTCA CTGCCTTGAA AGTGAGCCCA GGATGAGGTG GACGGCCAGC 
CAGCTCCTCG ACCATTCCTT CGTCAAGGTC TGCACAGATG AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_022418666.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274374.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

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CloneID	OCe285902
Clone ID Related Accession (Same CDS sequence)	XM_022418666.1
Accession Version	XM_022418666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGCAC TCGGAGCGTG 
CCTTCAGACG CACGGAGCCA CGGCAGCCCT GCTGCTGCTC CTGTCCCTGC TGCCGCCGCC 
ACCGCCGGTC GCCCCGGCCC CGCAGGCAGC GACTCTGCAC CACCCAAACC CATCAGCAGT 
GCCCATGATA CCAGGGGTTC CAGTGTCCCT GAGAATGACC GGTTGGCTTC TATAGCCGCC 
GAGTTACAGT TTCGGTCCTT GAGCCGCCAC TCAAGCCCCA CGGAGGAGCG CGACGAACCA 
GCGTATCCAA AAGGTGATTC AAGTGGGTCA GCTCGAAGAA GTTGGGAACT TCGGACACTT 
ATCAGCCAGA CTAAGGACAG TGCTTCCAAA CAAGGACCCA TAGAAGCTAT CCAGAAATCA 
GTCCGACTGT TTGAAGAAAA GAGGTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGATA CCCCTAAATC ATATGATAAC GTTATGCATG TAGGTTTGAG GAAGGTGACC 
TTCAAGTGGC AAAGAGGAAA CAAAATTGGG GAGGGGCAGT ATGGGAAGGT CTACACCTGT 
ATCAGTGTTG ACACTGGCGA ATTGATGGCT ATGAAGGAGA TCCGATTTCA ACCTAATGAC 
CATAAAACTA TCAAGGAAAC TGCAGACGAA TTGAAAATCT TTGAGGGCAT CAAACACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAAGCA CAGTAACTTT GCCTAATTAT 
CAGGAAATCT CCATGAGTTA CATCATTTCT GCCTTCTCTC TGAAACTAGA GGAGTTCTTC 
CTAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274374.1
CDS	187..4515
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAPVPAAAATAGRPGPAGSDSAPPKPISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHRSTVTLPNYQEISMSYIISAFSLKLEEFFLK

Target ORF information:

RefSeq Version	XM_022418666.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418666.1

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGCAC TCGGAGCGTG 
CCTTCAGACG CACGGAGCCA CGGCAGCCCT GCTGCTGCTC CTGTCCCTGC TGCCGCCGCC 
ACCGCCGGTC GCCCCGGCCC CGCAGGCAGC GACTCTGCAC CACCCAAACC CATCAGCAGT 
GCCCATGATA CCAGGGGTTC CAGTGTCCCT GAGAATGACC GGTTGGCTTC TATAGCCGCC 
GAGTTACAGT TTCGGTCCTT GAGCCGCCAC TCAAGCCCCA CGGAGGAGCG CGACGAACCA 
GCGTATCCAA AAGGTGATTC AAGTGGGTCA GCTCGAAGAA GTTGGGAACT TCGGACACTT 
ATCAGCCAGA CTAAGGACAG TGCTTCCAAA CAAGGACCCA TAGAAGCTAT CCAGAAATCA 
GTCCGACTGT TTGAAGAAAA GAGGTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGATA CCCCTAAATC ATATGATAAC GTTATGCATG TAGGTTTGAG GAAGGTGACC 
TTCAAGTGGC AAAGAGGAAA CAAAATTGGG GAGGGGCAGT ATGGGAAGGT CTACACCTGT 
ATCAGTGTTG ACACTGGCGA ATTGATGGCT ATGAAGGAGA TCCGATTTCA ACCTAATGAC 
CATAAAACTA TCAAGGAAAC TGCAGACGAA TTGAAAATCT TTGAGGGCAT CAAACACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAAGCA CAGTAACTTT GCCTAATTAT 
CAGGAAATCT CCATGAGTTA CATCATTTCT GCCTTCTCTC TGAAACTAGA GGAGTTCTTC 
CTAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014113044.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968519.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

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CloneID	OCe34474
Clone ID Related Accession (Same CDS sequence)	XM_014113044.1 , XM_014113044.2
Accession Version	XM_014113044.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGCAC TCGGAGCGTG 
CCTTCAGACG CACGGAGCCA CGGCAGCCCT GCTGCTGCTC CTGTCCCTGC TGCCGCCGCC 
ACCGCCGGTC GCCCCGGCCC CGCAGGCAGC GACTCTGCAC CACCCAAACC CATCAGCAGT 
GCCCATGATA CCAGGGGTTC CAGTGTCCCT GAGAATGACC GGTTGGCTTC TATAGCCGCC 
GAGTTACAGT TTCGGTCCTT GAGCCGCCAC TCAAGCCCCA CGGAGGAGCG CGACGAACCA 
GCGTATCCAA AAGGTGATTC AAGTGGGTCA GCTCGAAGAA GTTGGGAACT TCGGACACTT 
ATCAGCCAGA CTAAGGACAG TGCTTCCAAA CAAGGACCCA TAGAAGCTAT CCAGAAATCA 
GTCCGACTGT TTGAAGAAAA GAGGTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGATA CCCCTAAATC ATATGATAAC GTTATGCATG TAGGTTTGAG GAAGGTGACC 
TTCAAGTGGC AAAGAGGAAA CAAAATTGGG GAGGGGCAGT ATGGGAAGGT CTACACCTGT 
ATCAGTGTTG ACACTGGCGA ATTGATGGCT ATGAAGGAGA TCCGATTTCA ACCTAATGAC 
CATAAAACTA TCAAGGAAAC TGCAGACGAA TTGAAAATCT TTGAGGGCAT CAAACACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAGAAG AAATGTACAT CTTCATGGAA 
TACTGTGACG AGGGGACATT AGAAGAGGTG TCAAGGCTGG GACTTCAGGA GCATGTCATT 
AGGCTGTATT CAAAGCAGAT CACCATCGCA ATCAACGTCC TCCATGAGCA TGGCATCGTT 
CACCGTGACA TCAAAGGTGC CAATATCTTC CTAACCTCCT CTGGACTCAT CAAGCTGGGG 
GATTTTGGAT GCTCAGTAAA GCTTAAAAAC AACGCACAGA CCATGCCGGG AGAAGTGAAT 
AGCACACTGG GGACAGCAGC ATACATGGCT CCAGAAGTCA TCACTCGTGC AAAAGGAGAA 
GGCCATGGGC GTGCCGCTGA TATCTGGAGT CTGGGCTGTG TCGTCATAGA GATGGTGACC 
GGCAAGAGGC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTATAA AGTGGGAATG 
GGGCACAAGC CACCCATCCC TGAGAGACTA AGCCCGGAAG GAAAGGACTT CCTTTCTCAC 
TGCCTTGAAA GTGAGCCCAG GATGAGGTGG ACGGCCAGCC AGCTCCTCGA CCATTCCTTC 
GTCAAGGTCT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968519.1
CDS	182..5008
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAPVPAAAATAGRPGPAGSDSAPPKPISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014113044.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113044.1

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGCAC TCGGAGCGTG 
CCTTCAGACG CACGGAGCCA CGGCAGCCCT GCTGCTGCTC CTGTCCCTGC TGCCGCCGCC 
ACCGCCGGTC GCCCCGGCCC CGCAGGCAGC GACTCTGCAC CACCCAAACC CATCAGCAGT 
GCCCATGATA CCAGGGGTTC CAGTGTCCCT GAGAATGACC GGTTGGCTTC TATAGCCGCC 
GAGTTACAGT TTCGGTCCTT GAGCCGCCAC TCAAGCCCCA CGGAGGAGCG CGACGAACCA 
GCGTATCCAA AAGGTGATTC AAGTGGGTCA GCTCGAAGAA GTTGGGAACT TCGGACACTT 
ATCAGCCAGA CTAAGGACAG TGCTTCCAAA CAAGGACCCA TAGAAGCTAT CCAGAAATCA 
GTCCGACTGT TTGAAGAAAA GAGGTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGATA CCCCTAAATC ATATGATAAC GTTATGCATG TAGGTTTGAG GAAGGTGACC 
TTCAAGTGGC AAAGAGGAAA CAAAATTGGG GAGGGGCAGT ATGGGAAGGT CTACACCTGT 
ATCAGTGTTG ACACTGGCGA ATTGATGGCT ATGAAGGAGA TCCGATTTCA ACCTAATGAC 
CATAAAACTA TCAAGGAAAC TGCAGACGAA TTGAAAATCT TTGAGGGCAT CAAACACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAGAAG AAATGTACAT CTTCATGGAA 
TACTGTGACG AGGGGACATT AGAAGAGGTG TCAAGGCTGG GACTTCAGGA GCATGTCATT 
AGGCTGTATT CAAAGCAGAT CACCATCGCA ATCAACGTCC TCCATGAGCA TGGCATCGTT 
CACCGTGACA TCAAAGGTGC CAATATCTTC CTAACCTCCT CTGGACTCAT CAAGCTGGGG 
GATTTTGGAT GCTCAGTAAA GCTTAAAAAC AACGCACAGA CCATGCCGGG AGAAGTGAAT 
AGCACACTGG GGACAGCAGC ATACATGGCT CCAGAAGTCA TCACTCGTGC AAAAGGAGAA 
GGCCATGGGC GTGCCGCTGA TATCTGGAGT CTGGGCTGTG TCGTCATAGA GATGGTGACC 
GGCAAGAGGC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTATAA AGTGGGAATG 
GGGCACAAGC CACCCATCCC TGAGAGACTA AGCCCGGAAG GAAAGGACTT CCTTTCTCAC 
TGCCTTGAAA GTGAGCCCAG GATGAGGTGG ACGGCCAGCC AGCTCCTCGA CCATTCCTTC 
GTCAAGGTCT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014113044.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968519.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

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CloneID	OCe34474
Clone ID Related Accession (Same CDS sequence)	XM_014113044.1 , XM_014113044.2
Accession Version	XM_014113044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGCAC TCGGAGCGTG 
CCTTCAGACG CACGGAGCCA CGGCAGCCCT GCTGCTGCTC CTGTCCCTGC TGCCGCCGCC 
ACCGCCGGTC GCCCCGGCCC CGCAGGCAGC GACTCTGCAC CACCCAAACC CATCAGCAGT 
GCCCATGATA CCAGGGGTTC CAGTGTCCCT GAGAATGACC GGTTGGCTTC TATAGCCGCC 
GAGTTACAGT TTCGGTCCTT GAGCCGCCAC TCAAGCCCCA CGGAGGAGCG CGACGAACCA 
GCGTATCCAA AAGGTGATTC AAGTGGGTCA GCTCGAAGAA GTTGGGAACT TCGGACACTT 
ATCAGCCAGA CTAAGGACAG TGCTTCCAAA CAAGGACCCA TAGAAGCTAT CCAGAAATCA 
GTCCGACTGT TTGAAGAAAA GAGGTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGATA CCCCTAAATC ATATGATAAC GTTATGCATG TAGGTTTGAG GAAGGTGACC 
TTCAAGTGGC AAAGAGGAAA CAAAATTGGG GAGGGGCAGT ATGGGAAGGT CTACACCTGT 
ATCAGTGTTG ACACTGGCGA ATTGATGGCT ATGAAGGAGA TCCGATTTCA ACCTAATGAC 
CATAAAACTA TCAAGGAAAC TGCAGACGAA TTGAAAATCT TTGAGGGCAT CAAACACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAGAAG AAATGTACAT CTTCATGGAA 
TACTGTGACG AGGGGACATT AGAAGAGGTG TCAAGGCTGG GACTTCAGGA GCATGTCATT 
AGGCTGTATT CAAAGCAGAT CACCATCGCA ATCAACGTCC TCCATGAGCA TGGCATCGTT 
CACCGTGACA TCAAAGGTGC CAATATCTTC CTAACCTCCT CTGGACTCAT CAAGCTGGGG 
GATTTTGGAT GCTCAGTAAA GCTTAAAAAC AACGCACAGA CCATGCCGGG AGAAGTGAAT 
AGCACACTGG GGACAGCAGC ATACATGGCT CCAGAAGTCA TCACTCGTGC AAAAGGAGAA 
GGCCATGGGC GTGCCGCTGA TATCTGGAGT CTGGGCTGTG TCGTCATAGA GATGGTGACC 
GGCAAGAGGC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTATAA AGTGGGAATG 
GGGCACAAGC CACCCATCCC TGAGAGACTA AGCCCGGAAG GAAAGGACTT CCTTTCTCAC 
TGCCTTGAAA GTGAGCCCAG GATGAGGTGG ACGGCCAGCC AGCTCCTCGA CCATTCCTTC 
GTCAAGGTCT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968519.1
CDS	187..5013
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAPVPAAAATAGRPGPAGSDSAPPKPISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014113044.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113044.2

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGCAC TCGGAGCGTG 
CCTTCAGACG CACGGAGCCA CGGCAGCCCT GCTGCTGCTC CTGTCCCTGC TGCCGCCGCC 
ACCGCCGGTC GCCCCGGCCC CGCAGGCAGC GACTCTGCAC CACCCAAACC CATCAGCAGT 
GCCCATGATA CCAGGGGTTC CAGTGTCCCT GAGAATGACC GGTTGGCTTC TATAGCCGCC 
GAGTTACAGT TTCGGTCCTT GAGCCGCCAC TCAAGCCCCA CGGAGGAGCG CGACGAACCA 
GCGTATCCAA AAGGTGATTC AAGTGGGTCA GCTCGAAGAA GTTGGGAACT TCGGACACTT 
ATCAGCCAGA CTAAGGACAG TGCTTCCAAA CAAGGACCCA TAGAAGCTAT CCAGAAATCA 
GTCCGACTGT TTGAAGAAAA GAGGTATCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 
GTTTGTGATA CCCCTAAATC ATATGATAAC GTTATGCATG TAGGTTTGAG GAAGGTGACC 
TTCAAGTGGC AAAGAGGAAA CAAAATTGGG GAGGGGCAGT ATGGGAAGGT CTACACCTGT 
ATCAGTGTTG ACACTGGCGA ATTGATGGCT ATGAAGGAGA TCCGATTTCA ACCTAATGAC 
CATAAAACTA TCAAGGAAAC TGCAGACGAA TTGAAAATCT TTGAGGGCAT CAAACACCCC 
AATCTGGTTC GGTATTTTGG TGTGGAGCTT CATAGAGAAG AAATGTACAT CTTCATGGAA 
TACTGTGACG AGGGGACATT AGAAGAGGTG TCAAGGCTGG GACTTCAGGA GCATGTCATT 
AGGCTGTATT CAAAGCAGAT CACCATCGCA ATCAACGTCC TCCATGAGCA TGGCATCGTT 
CACCGTGACA TCAAAGGTGC CAATATCTTC CTAACCTCCT CTGGACTCAT CAAGCTGGGG 
GATTTTGGAT GCTCAGTAAA GCTTAAAAAC AACGCACAGA CCATGCCGGG AGAAGTGAAT 
AGCACACTGG GGACAGCAGC ATACATGGCT CCAGAAGTCA TCACTCGTGC AAAAGGAGAA 
GGCCATGGGC GTGCCGCTGA TATCTGGAGT CTGGGCTGTG TCGTCATAGA GATGGTGACC 
GGCAAGAGGC CTTGGCATGA ATATGAACAC AACTTTCAGA TTATGTATAA AGTGGGAATG 
GGGCACAAGC CACCCATCCC TGAGAGACTA AGCCCGGAAG GAAAGGACTT CCTTTCTCAC 
TGCCTTGAAA GTGAGCCCAG GATGAGGTGG ACGGCCAGCC AGCTCCTCGA CCATTCCTTC 
GTCAAGGTCT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014113046.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968521.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

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CloneID	OCe34475
Clone ID Related Accession (Same CDS sequence)	XM_014113046.2 , XM_014113046.1
Accession Version	XM_014113046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGCT 
ATTCATCGGA ACAGTCCCCG CCCTGTGAAG GTACCACGAT GCCATAGTGA CCCTCCCAAC 
CCACATCTAA TTATCCCGAC TCCAGAGGGA TTCAGCACTC GGAGCGTGCC TTCAGACGCA 
CGGAGCCACG GCAGCCCTGC TGCTGCTCCT GTCCCTGCTG CCGCCGCCAC CGCCGGTCGC 
CCCGGCCCCG CAGGCAGCGA CTCTGCACCA CCCAAACCCA TCAGCAGTGC CCATGATACC 
AGGGGTTCCA GTGTCCCTGA GAATGACCGG TTGGCTTCTA TAGCCGCCGA GTTACAGTTT 
CGGTCCTTGA GCCGCCACTC AAGCCCCACG GAGGAGCGCG ACGAACCAGC GTATCCAAAA 
GGTGATTCAA GTGGGTCAGC TCGAAGAAGT TGGGAACTTC GGACACTTAT CAGCCAGACT 
AAGGACAGTG CTTCCAAACA AGGACCCATA GAAGCTATCC AGAAATCAGT CCGACTGTTT 
GAAGAAAAGA GGTATCGAGA AATGAGGAGA AAGAATATCA TTGGTCAAGT TTGTGATACC 
CCTAAATCAT ATGATAACGT TATGCATGTA GGTTTGAGGA AGGTGACCTT CAAGTGGCAA 
AGAGGAAACA AAATTGGGGA GGGGCAGTAT GGGAAGGTCT ACACCTGTAT CAGTGTTGAC 
ACTGGCGAAT TGATGGCTAT GAAGGAGATC CGATTTCAAC CTAATGACCA TAAAACTATC 
AAGGAAACTG CAGACGAATT GAAAATCTTT GAGGGCATCA AACACCCCAA TCTGGTTCGG 
TATTTTGGTG TGGAGCTTCA TAGAGAAGAA ATGTACATCT TCATGGAATA CTGTGACGAG 
GGGACATTAG AAGAGGTGTC AAGGCTGGGA CTTCAGGAGC ATGTCATTAG GCTGTATTCA 
AAGCAGATCA CCATCGCAAT CAACGTCCTC CATGAGCATG GCATCGTTCA CCGTGACATC 
AAAGGTGCCA ATATCTTCCT AACCTCCTCT GGACTCATCA AGCTGGGGGA TTTTGGATGC 
TCAGTAAAGC TTAAAAACAA CGCACAGACC ATGCCGGGAG AAGTGAATAG CACACTGGGG 
ACAGCAGCAT ACATGGCTCC AGAAGTCATC ACTCGTGCAA AAGGAGAAGG CCATGGGCGT 
GCCGCTGATA TCTGGAGTCT GGGCTGTGTC GTCATAGAGA TGGTGACCGG CAAGAGGCCT 
TGGCATGAAT ATGAACACAA CTTTCAGATT ATGTATAAAG TGGGAATGGG GCACAAGCCA 
CCCATCCCTG AGAGACTAAG CCCGGAAGGA AAGGACTTCC TTTCTCACTG CCTTGAAAGT 
GAGCCCAGGA TGAGGTGGAC GGCCAGCCAG CTCCTCGACC ATTCCTTCGT CAAGGTCTGC 
ACAGATGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968521.1
CDS	187..5001
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAPVPAAAATAGRPGPAGSDSAPPKPISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014113046.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113046.2

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGCT 
ATTCATCGGA ACAGTCCCCG CCCTGTGAAG GTACCACGAT GCCATAGTGA CCCTCCCAAC 
CCACATCTAA TTATCCCGAC TCCAGAGGGA TTCAGCACTC GGAGCGTGCC TTCAGACGCA 
CGGAGCCACG GCAGCCCTGC TGCTGCTCCT GTCCCTGCTG CCGCCGCCAC CGCCGGTCGC 
CCCGGCCCCG CAGGCAGCGA CTCTGCACCA CCCAAACCCA TCAGCAGTGC CCATGATACC 
AGGGGTTCCA GTGTCCCTGA GAATGACCGG TTGGCTTCTA TAGCCGCCGA GTTACAGTTT 
CGGTCCTTGA GCCGCCACTC AAGCCCCACG GAGGAGCGCG ACGAACCAGC GTATCCAAAA 
GGTGATTCAA GTGGGTCAGC TCGAAGAAGT TGGGAACTTC GGACACTTAT CAGCCAGACT 
AAGGACAGTG CTTCCAAACA AGGACCCATA GAAGCTATCC AGAAATCAGT CCGACTGTTT 
GAAGAAAAGA GGTATCGAGA AATGAGGAGA AAGAATATCA TTGGTCAAGT TTGTGATACC 
CCTAAATCAT ATGATAACGT TATGCATGTA GGTTTGAGGA AGGTGACCTT CAAGTGGCAA 
AGAGGAAACA AAATTGGGGA GGGGCAGTAT GGGAAGGTCT ACACCTGTAT CAGTGTTGAC 
ACTGGCGAAT TGATGGCTAT GAAGGAGATC CGATTTCAAC CTAATGACCA TAAAACTATC 
AAGGAAACTG CAGACGAATT GAAAATCTTT GAGGGCATCA AACACCCCAA TCTGGTTCGG 
TATTTTGGTG TGGAGCTTCA TAGAGAAGAA ATGTACATCT TCATGGAATA CTGTGACGAG 
GGGACATTAG AAGAGGTGTC AAGGCTGGGA CTTCAGGAGC ATGTCATTAG GCTGTATTCA 
AAGCAGATCA CCATCGCAAT CAACGTCCTC CATGAGCATG GCATCGTTCA CCGTGACATC 
AAAGGTGCCA ATATCTTCCT AACCTCCTCT GGACTCATCA AGCTGGGGGA TTTTGGATGC 
TCAGTAAAGC TTAAAAACAA CGCACAGACC ATGCCGGGAG AAGTGAATAG CACACTGGGG 
ACAGCAGCAT ACATGGCTCC AGAAGTCATC ACTCGTGCAA AAGGAGAAGG CCATGGGCGT 
GCCGCTGATA TCTGGAGTCT GGGCTGTGTC GTCATAGAGA TGGTGACCGG CAAGAGGCCT 
TGGCATGAAT ATGAACACAA CTTTCAGATT ATGTATAAAG TGGGAATGGG GCACAAGCCA 
CCCATCCCTG AGAGACTAAG CCCGGAAGGA AAGGACTTCC TTTCTCACTG CCTTGAAAGT 
GAGCCCAGGA TGAGGTGGAC GGCCAGCCAG CTCCTCGACC ATTCCTTCGT CAAGGTCTGC 
ACAGATGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014113046.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968521.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe34475
Clone ID Related Accession (Same CDS sequence)	XM_014113046.2 , XM_014113046.1
Accession Version	XM_014113046.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGCT 
ATTCATCGGA ACAGTCCCCG CCCTGTGAAG GTACCACGAT GCCATAGTGA CCCTCCCAAC 
CCACATCTAA TTATCCCGAC TCCAGAGGGA TTCAGCACTC GGAGCGTGCC TTCAGACGCA 
CGGAGCCACG GCAGCCCTGC TGCTGCTCCT GTCCCTGCTG CCGCCGCCAC CGCCGGTCGC 
CCCGGCCCCG CAGGCAGCGA CTCTGCACCA CCCAAACCCA TCAGCAGTGC CCATGATACC 
AGGGGTTCCA GTGTCCCTGA GAATGACCGG TTGGCTTCTA TAGCCGCCGA GTTACAGTTT 
CGGTCCTTGA GCCGCCACTC AAGCCCCACG GAGGAGCGCG ACGAACCAGC GTATCCAAAA 
GGTGATTCAA GTGGGTCAGC TCGAAGAAGT TGGGAACTTC GGACACTTAT CAGCCAGACT 
AAGGACAGTG CTTCCAAACA AGGACCCATA GAAGCTATCC AGAAATCAGT CCGACTGTTT 
GAAGAAAAGA GGTATCGAGA AATGAGGAGA AAGAATATCA TTGGTCAAGT TTGTGATACC 
CCTAAATCAT ATGATAACGT TATGCATGTA GGTTTGAGGA AGGTGACCTT CAAGTGGCAA 
AGAGGAAACA AAATTGGGGA GGGGCAGTAT GGGAAGGTCT ACACCTGTAT CAGTGTTGAC 
ACTGGCGAAT TGATGGCTAT GAAGGAGATC CGATTTCAAC CTAATGACCA TAAAACTATC 
AAGGAAACTG CAGACGAATT GAAAATCTTT GAGGGCATCA AACACCCCAA TCTGGTTCGG 
TATTTTGGTG TGGAGCTTCA TAGAGAAGAA ATGTACATCT TCATGGAATA CTGTGACGAG 
GGGACATTAG AAGAGGTGTC AAGGCTGGGA CTTCAGGAGC ATGTCATTAG GCTGTATTCA 
AAGCAGATCA CCATCGCAAT CAACGTCCTC CATGAGCATG GCATCGTTCA CCGTGACATC 
AAAGGTGCCA ATATCTTCCT AACCTCCTCT GGACTCATCA AGCTGGGGGA TTTTGGATGC 
TCAGTAAAGC TTAAAAACAA CGCACAGACC ATGCCGGGAG AAGTGAATAG CACACTGGGG 
ACAGCAGCAT ACATGGCTCC AGAAGTCATC ACTCGTGCAA AAGGAGAAGG CCATGGGCGT 
GCCGCTGATA TCTGGAGTCT GGGCTGTGTC GTCATAGAGA TGGTGACCGG CAAGAGGCCT 
TGGCATGAAT ATGAACACAA CTTTCAGATT ATGTATAAAG TGGGAATGGG GCACAAGCCA 
CCCATCCCTG AGAGACTAAG CCCGGAAGGA AAGGACTTCC TTTCTCACTG CCTTGAAAGT 
GAGCCCAGGA TGAGGTGGAC GGCCAGCCAG CTCCTCGACC ATTCCTTCGT CAAGGTCTGC 
ACAGATGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968521.1
CDS	182..4996
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAPVPAAAATAGRPGPAGSDSAPPKPISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014113046.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113046.1

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGCT 
ATTCATCGGA ACAGTCCCCG CCCTGTGAAG GTACCACGAT GCCATAGTGA CCCTCCCAAC 
CCACATCTAA TTATCCCGAC TCCAGAGGGA TTCAGCACTC GGAGCGTGCC TTCAGACGCA 
CGGAGCCACG GCAGCCCTGC TGCTGCTCCT GTCCCTGCTG CCGCCGCCAC CGCCGGTCGC 
CCCGGCCCCG CAGGCAGCGA CTCTGCACCA CCCAAACCCA TCAGCAGTGC CCATGATACC 
AGGGGTTCCA GTGTCCCTGA GAATGACCGG TTGGCTTCTA TAGCCGCCGA GTTACAGTTT 
CGGTCCTTGA GCCGCCACTC AAGCCCCACG GAGGAGCGCG ACGAACCAGC GTATCCAAAA 
GGTGATTCAA GTGGGTCAGC TCGAAGAAGT TGGGAACTTC GGACACTTAT CAGCCAGACT 
AAGGACAGTG CTTCCAAACA AGGACCCATA GAAGCTATCC AGAAATCAGT CCGACTGTTT 
GAAGAAAAGA GGTATCGAGA AATGAGGAGA AAGAATATCA TTGGTCAAGT TTGTGATACC 
CCTAAATCAT ATGATAACGT TATGCATGTA GGTTTGAGGA AGGTGACCTT CAAGTGGCAA 
AGAGGAAACA AAATTGGGGA GGGGCAGTAT GGGAAGGTCT ACACCTGTAT CAGTGTTGAC 
ACTGGCGAAT TGATGGCTAT GAAGGAGATC CGATTTCAAC CTAATGACCA TAAAACTATC 
AAGGAAACTG CAGACGAATT GAAAATCTTT GAGGGCATCA AACACCCCAA TCTGGTTCGG 
TATTTTGGTG TGGAGCTTCA TAGAGAAGAA ATGTACATCT TCATGGAATA CTGTGACGAG 
GGGACATTAG AAGAGGTGTC AAGGCTGGGA CTTCAGGAGC ATGTCATTAG GCTGTATTCA 
AAGCAGATCA CCATCGCAAT CAACGTCCTC CATGAGCATG GCATCGTTCA CCGTGACATC 
AAAGGTGCCA ATATCTTCCT AACCTCCTCT GGACTCATCA AGCTGGGGGA TTTTGGATGC 
TCAGTAAAGC TTAAAAACAA CGCACAGACC ATGCCGGGAG AAGTGAATAG CACACTGGGG 
ACAGCAGCAT ACATGGCTCC AGAAGTCATC ACTCGTGCAA AAGGAGAAGG CCATGGGCGT 
GCCGCTGATA TCTGGAGTCT GGGCTGTGTC GTCATAGAGA TGGTGACCGG CAAGAGGCCT 
TGGCATGAAT ATGAACACAA CTTTCAGATT ATGTATAAAG TGGGAATGGG GCACAAGCCA 
CCCATCCCTG AGAGACTAAG CCCGGAAGGA AAGGACTTCC TTTCTCACTG CCTTGAAAGT 
GAGCCCAGGA TGAGGTGGAC GGCCAGCCAG CTCCTCGACC ATTCCTTCGT CAAGGTCTGC 
ACAGATGAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014113049.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968524.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

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CloneID	OCe34476
Clone ID Related Accession (Same CDS sequence)	XM_014113049.2 , XM_014113049.1
Accession Version	XM_014113049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGGGG TTCCAGTGTC 
CCTGAGAATG ACCGGTTGGC TTCTATAGCC GCCGAGTTAC AGTTTCGGTC CTTGAGCCGC 
CACTCAAGCC CCACGGAGGA GCGCGACGAA CCAGCGTATC CAAAAGGTGA TTCAAGTGGG 
TCAGCTCGAA GAAGTTGGGA ACTTCGGACA CTTATCAGCC AGACTAAGGA CAGTGCTTCC 
AAACAAGGAC CCATAGAAGC TATCCAGAAA TCAGTCCGAC TGTTTGAAGA AAAGAGGTAT 
CGAGAAATGA GGAGAAAGAA TATCATTGGT CAAGTTTGTG ATACCCCTAA ATCATATGAT 
AACGTTATGC ATGTAGGTTT GAGGAAGGTG ACCTTCAAGT GGCAAAGAGG AAACAAAATT 
GGGGAGGGGC AGTATGGGAA GGTCTACACC TGTATCAGTG TTGACACTGG CGAATTGATG 
GCTATGAAGG AGATCCGATT TCAACCTAAT GACCATAAAA CTATCAAGGA AACTGCAGAC 
GAATTGAAAA TCTTTGAGGG CATCAAACAC CCCAATCTGG TTCGGTATTT TGGTGTGGAG 
CTTCATAGAG AAGAAATGTA CATCTTCATG GAATACTGTG ACGAGGGGAC ATTAGAAGAG 
GTGTCAAGGC TGGGACTTCA GGAGCATGTC ATTAGGCTGT ATTCAAAGCA GATCACCATC 
GCAATCAACG TCCTCCATGA GCATGGCATC GTTCACCGTG ACATCAAAGG TGCCAATATC 
TTCCTAACCT CCTCTGGACT CATCAAGCTG GGGGATTTTG GATGCTCAGT AAAGCTTAAA 
AACAACGCAC AGACCATGCC GGGAGAAGTG AATAGCACAC TGGGGACAGC AGCATACATG 
GCTCCAGAAG TCATCACTCG TGCAAAAGGA GAAGGCCATG GGCGTGCCGC TGATATCTGG 
AGTCTGGGCT GTGTCGTCAT AGAGATGGTG ACCGGCAAGA GGCCTTGGCA TGAATATGAA 
CACAACTTTC AGATTATGTA TAAAGTGGGA ATGGGGCACA AGCCACCCAT CCCTGAGAGA 
CTAAGCCCGG AAGGAAAGGA CTTCCTTTCT CACTGCCTTG AAAGTGAGCC CAGGATGAGG 
TGGACGGCCA GCCAGCTCCT CGACCATTCC TTCGTCAAGG TCTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968524.1
CDS	187..4866
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014113049.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113049.2

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGGGG TTCCAGTGTC 
CCTGAGAATG ACCGGTTGGC TTCTATAGCC GCCGAGTTAC AGTTTCGGTC CTTGAGCCGC 
CACTCAAGCC CCACGGAGGA GCGCGACGAA CCAGCGTATC CAAAAGGTGA TTCAAGTGGG 
TCAGCTCGAA GAAGTTGGGA ACTTCGGACA CTTATCAGCC AGACTAAGGA CAGTGCTTCC 
AAACAAGGAC CCATAGAAGC TATCCAGAAA TCAGTCCGAC TGTTTGAAGA AAAGAGGTAT 
CGAGAAATGA GGAGAAAGAA TATCATTGGT CAAGTTTGTG ATACCCCTAA ATCATATGAT 
AACGTTATGC ATGTAGGTTT GAGGAAGGTG ACCTTCAAGT GGCAAAGAGG AAACAAAATT 
GGGGAGGGGC AGTATGGGAA GGTCTACACC TGTATCAGTG TTGACACTGG CGAATTGATG 
GCTATGAAGG AGATCCGATT TCAACCTAAT GACCATAAAA CTATCAAGGA AACTGCAGAC 
GAATTGAAAA TCTTTGAGGG CATCAAACAC CCCAATCTGG TTCGGTATTT TGGTGTGGAG 
CTTCATAGAG AAGAAATGTA CATCTTCATG GAATACTGTG ACGAGGGGAC ATTAGAAGAG 
GTGTCAAGGC TGGGACTTCA GGAGCATGTC ATTAGGCTGT ATTCAAAGCA GATCACCATC 
GCAATCAACG TCCTCCATGA GCATGGCATC GTTCACCGTG ACATCAAAGG TGCCAATATC 
TTCCTAACCT CCTCTGGACT CATCAAGCTG GGGGATTTTG GATGCTCAGT AAAGCTTAAA 
AACAACGCAC AGACCATGCC GGGAGAAGTG AATAGCACAC TGGGGACAGC AGCATACATG 
GCTCCAGAAG TCATCACTCG TGCAAAAGGA GAAGGCCATG GGCGTGCCGC TGATATCTGG 
AGTCTGGGCT GTGTCGTCAT AGAGATGGTG ACCGGCAAGA GGCCTTGGCA TGAATATGAA 
CACAACTTTC AGATTATGTA TAAAGTGGGA ATGGGGCACA AGCCACCCAT CCCTGAGAGA 
CTAAGCCCGG AAGGAAAGGA CTTCCTTTCT CACTGCCTTG AAAGTGAGCC CAGGATGAGG 
TGGACGGCCA GCCAGCTCCT CGACCATTCC TTCGTCAAGG TCTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014113049.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968524.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

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CloneID	OCe34476
Clone ID Related Accession (Same CDS sequence)	XM_014113049.2 , XM_014113049.1
Accession Version	XM_014113049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGGGG TTCCAGTGTC 
CCTGAGAATG ACCGGTTGGC TTCTATAGCC GCCGAGTTAC AGTTTCGGTC CTTGAGCCGC 
CACTCAAGCC CCACGGAGGA GCGCGACGAA CCAGCGTATC CAAAAGGTGA TTCAAGTGGG 
TCAGCTCGAA GAAGTTGGGA ACTTCGGACA CTTATCAGCC AGACTAAGGA CAGTGCTTCC 
AAACAAGGAC CCATAGAAGC TATCCAGAAA TCAGTCCGAC TGTTTGAAGA AAAGAGGTAT 
CGAGAAATGA GGAGAAAGAA TATCATTGGT CAAGTTTGTG ATACCCCTAA ATCATATGAT 
AACGTTATGC ATGTAGGTTT GAGGAAGGTG ACCTTCAAGT GGCAAAGAGG AAACAAAATT 
GGGGAGGGGC AGTATGGGAA GGTCTACACC TGTATCAGTG TTGACACTGG CGAATTGATG 
GCTATGAAGG AGATCCGATT TCAACCTAAT GACCATAAAA CTATCAAGGA AACTGCAGAC 
GAATTGAAAA TCTTTGAGGG CATCAAACAC CCCAATCTGG TTCGGTATTT TGGTGTGGAG 
CTTCATAGAG AAGAAATGTA CATCTTCATG GAATACTGTG ACGAGGGGAC ATTAGAAGAG 
GTGTCAAGGC TGGGACTTCA GGAGCATGTC ATTAGGCTGT ATTCAAAGCA GATCACCATC 
GCAATCAACG TCCTCCATGA GCATGGCATC GTTCACCGTG ACATCAAAGG TGCCAATATC 
TTCCTAACCT CCTCTGGACT CATCAAGCTG GGGGATTTTG GATGCTCAGT AAAGCTTAAA 
AACAACGCAC AGACCATGCC GGGAGAAGTG AATAGCACAC TGGGGACAGC AGCATACATG 
GCTCCAGAAG TCATCACTCG TGCAAAAGGA GAAGGCCATG GGCGTGCCGC TGATATCTGG 
AGTCTGGGCT GTGTCGTCAT AGAGATGGTG ACCGGCAAGA GGCCTTGGCA TGAATATGAA 
CACAACTTTC AGATTATGTA TAAAGTGGGA ATGGGGCACA AGCCACCCAT CCCTGAGAGA 
CTAAGCCCGG AAGGAAAGGA CTTCCTTTCT CACTGCCTTG AAAGTGAGCC CAGGATGAGG 
TGGACGGCCA GCCAGCTCCT CGACCATTCC TTCGTCAAGG TCTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968524.1
CDS	182..4861
Translation	MREAAAAAELVPPPAFAVTPAAAAMEEPPPPPPGPEPEPEPEPEPERCRAARQECTVGDSACKNSESDPEDFSDEINTENLYGTSPPSTPRQMKRMSTKHQRNNVGKPANRSGLKEKMNAPNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDRETGETKNTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSFAFVRNGASFNGTSVEGQCRAPHGTKIVCYSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRSSKGNEPEDEGDDTEGDLKELDSSTDESEEEQLSGPRAPEPTQPIDTNFSIHSQDCVLKKLERLESEDDSFGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDHPVEFSEFPDPMWGSDYVQLSRTPPSSEQKGSTVSWDELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLADLQKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSNASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPIRDLLDVLKSKQYVKVQIPGLENLQVFVPDTLAEEKNIILQLLNAAAGKDCSKESDDVLIDAYLLLTKQSDRARDSEDSWASWEVRPVKIVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLRQEQTSSQPVIARALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014113049.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113049.1

ORF Insert Sequence:

ATGAGAGAAG CCGCCGCCGC CGCCGAGCTG GTCCCCCCGC CCGCCTTCGC CGTCACGCCC 
GCCGCCGCCG CCATGGAGGA GCCGCCGCCG CCGCCGCCGG GACCCGAGCC CGAGCCCGAG 
CCCGAGCCCG AGCCCGAGCG CTGCCGGGCG GCGAGGCAAG AGTGCACAGT GGGAGATTCA 
GCTTGCAAGA ATTCTGAGTC TGATCCAGAG GATTTTTCAG ATGAAATCAA TACGGAGAAC 
CTTTATGGCA CCTCTCCCCC CAGCACACCT CGGCAGATGA AACGCATGTC CACCAAACAC 
CAGAGGAATA ACGTGGGGAA GCCTGCCAAT CGATCTGGTT TGAAAGAAAA AATGAATGCA 
CCAAACCAGC CTCCACACAA AGACACTGGA AAAACCATGG AGAATGTGGA AGAATATAGC 
TATAAGCAGG AGAAGAAGAT CCGAGCTGCT CTTAGGACAA CAGAGCGTGA TCATAAGAAA 
AATGTGCAGT GCTCGTTCAT GTTGGACTCA GTGGGTGGGT CTTTGCCCAA AAAATCAATT 
CCAGATGTGG ATCTTAATAA GCCTTACCTC AGTCTTGGCT GCAGCAATGC TAAGCTTCCG 
GTATCTGTGC CCATGCCTAT ACCCAGAACT GCACGCCAGA CTTCTAGGAC TGACTGTCCA 
GCAGATCGCT TAAAGTTTTT TGAGACTTTA CGACTTCTGC TAAAGCTTAC CTCAGTCTCA 
AAGAAGAAGG ACAGGGAGAC AGGAGAAACG AAAAATACAT CTGCTTTCTG GTTTAACCGA 
TCTAATGAAC TGATCTGGTT AGAGCTACAA GCCTGGCACG CAGGACGGAC CATTAATGAC 
CAGGACCTCT TTTTATATAC AGCCCGTCAA GCCATCCCTG ATATTATTAA TGAAATCCTT 
ACTTTCAAAG TTAATTATGG GAGCTTTGCC TTTGTTAGAA ATGGAGCTAG TTTTAATGGT 
ACTTCAGTAG AAGGGCAGTG CAGAGCCCCT CATGGAACAA AAATTGTGTG TTACTCAACA 
TATCATGAGC ATCTCCAACG CCAGAGAGTG TCATTTGAGC AGGTAAAGCG GATAATGGAG 
CTGCTGGAGT ACATGGAAGC ACTTTATCCA TCGTTGCAGG CTCTTCAAAA GGACTATGAA 
AAATATGCTG CAAAAGACTT CCAGGACAGG GTGCAGGCAC TCTGTTTGTG GTTAAACATC 
ACAAAGGACT TAAATCAGAA GTTAAGGATT ATGGGCACTG TTTTGGGCAT CAAGAATTTA 
TCAGACATTG GCTGGCCAGT GTTTGAAATC CCTTCTCCTC GATCGTCCAA AGGCAATGAG 
CCAGAAGATG AGGGTGATGA CACAGAAGGG GACTTGAAAG AGTTGGATAG TAGTACGGAT 
GAGAGTGAAG AAGAACAACT CTCAGGTCCA AGGGCACCAG AGCCCACACA GCCCATTGAC 
ACCAATTTCA GCATCCATTC ACAGGATTGT GTACTGAAGA AGCTTGAGAG GCTTGAGTCT 
GAGGATGATT CTTTTGGCTG GGGAGCACCA GACTGCAGCA CAGAGGCAGG CTTTAGTAGA 
CATTGTCTGA CTTCTATTTA TAGGCCATTT GTAGACAAAG CCCTGAAGCA AATGGGGTTA 
AGAAAGTTAA TTTTAAGACT TCACAAGCTG ATGGACGGTT CCTTGCAAAG GGCACGTATA 
GCACTGGTAA AGAGTGACCA TCCAGTGGAG TTTTCCGAAT TTCCAGACCC CATGTGGGGT 
TCCGATTATG TGCAGTTATC CAGGACACCA CCTTCCTCGG AGCAGAAGGG CAGCACTGTG 
TCGTGGGACG AGCTAAAGTC CATGGACTTA CCCTCCTTCG AACCTGCCTT CCTGGTGCTC 
TGTCGAGTCC TTCTGAATGT GATACATGAG TGTCTGAAGT TAAGACTGGA GCAGAGGCCT 
GCTGGAGAAC CATCTCTCTT AAGTATTAAG CAGCTGGTGA GGGAGTGTAA AGAGGTGCTA 
AAGGGCGGCC TTCTGATGAA GCAGTACTAC CAGTTCATGC TGCACGAGGT GCTGGCCGAC 
CTGCAGAAGA CTGACTGCAA CATCGACGCC TTTGAGGAGG ACCTGCACAA AATGCTGATG 
GTGTATTTTG ATTACATGAG AAGCTGGATC CAAATGCTGC AGCAGTTACC TCAAGCATCC 
CATAGTCTAA AAAACCTGTT AGAAGAAGAG TGGAATTTCA CCAAAGAAAT AACCCATTAC 
ATCCGGGGAG GAGAAGCCCA GGCTGGAAAA CTTTTCTGTG ACATTGCAGG GATGCTGCTG 
AAGTCAACAG GGAGTTTTTT AGAGTTCGGC TTGCAGGAGA GCTGTGCAGA GTTCTGGACC 
AGCGCTGACG ACTCCAATGC CTCAGACGAA ATAAGGAGAT CCGTTATAGA GATCAGTCGA 
GCTCTGAAGG AGCTTTTTCA CGAAGCTAGA GAAAGAGCCT CCAAGGCGCT TGGATTTGCT 
AAAATGTTGA GAAAGGACCT GGAAATAGCA GCAGAGTTCA TACTTTCAGC CCCCATTAGA 
GACCTTCTGG ATGTTCTGAA ATCAAAACAG TATGTTAAGG TACAGATTCC TGGCTTAGAA 
AACTTGCAAG TGTTTGTTCC AGACACTCTT GCTGAGGAGA AGAACATTAT TTTGCAGTTA 
CTCAATGCAG CCGCAGGAAA AGATTGCTCG AAAGAGTCAG ATGATGTACT GATTGATGCC 
TATCTGTTGC TAACCAAGCA GAGTGATCGC GCTCGGGATT CAGAGGATAG TTGGGCCTCA 
TGGGAAGTGC GACCTGTCAA AATTGTGCCT CAGGTCGAGA CCGTCGACAC CCTGAGAAGC 
ATGCAGGTGG ATAACCTTTT ACTAGTTGTC ATGCAGTCTG CACATCTCAC CATCCAGAGA 
AAAGCTTTCC AGCAGTCCAT CGAGGGACTC ATGACTCTCC GCCAGGAGCA GACGTCCAGT 
CAGCCGGTCA TCGCCAGAGC TTTACAGCAG TTAAAAAATG ATGCACTAGA GCTTTGCAAC 
AGAATAAGCG ATGCCATTGA CCGAGTGGAC CACATGTTCA CTTCAGAATT TGATGCGGAA 
GTTGATGAAT CAGAGTCTGT CACATTGCAA CAGTACTACC GAGAGGCAAT GATTCAGGGC 
TACAACTTTG GGTTCGAGTA TCATAAAGAA GTTGTTCGTT TGATGTCTGG AGAATTTAGA 
CAGAAGATAG GAGACAAATA TATAAGCTTT GCCCGGAAAT GGATGAACTA TGTCCTAACT 
AAATGTGAGA GTGGTCGAGG TACAAGACCC AGGTGGGCAA CCCAAGGTTT TGACTTTCTA 
CAAGCCATTG AACCTGCCTT TATTTCAGCT TTGCCAGAAG ATGACTTCTT GAGTCTTCAA 
GCCTTGATGA ATGAGTGCAT TGGCCATGTG ATAGGAAAGC CACACAGTCC TGTTACAGGT 
TTGTACCTTG CTATTCATCG GAACAGTCCC CGCCCTGTGA AGGTACCACG ATGCCATAGT 
GACCCTCCCA ACCCACATCT AATTATCCCG ACTCCAGAGG GATTCAGGGG TTCCAGTGTC 
CCTGAGAATG ACCGGTTGGC TTCTATAGCC GCCGAGTTAC AGTTTCGGTC CTTGAGCCGC 
CACTCAAGCC CCACGGAGGA GCGCGACGAA CCAGCGTATC CAAAAGGTGA TTCAAGTGGG 
TCAGCTCGAA GAAGTTGGGA ACTTCGGACA CTTATCAGCC AGACTAAGGA CAGTGCTTCC 
AAACAAGGAC CCATAGAAGC TATCCAGAAA TCAGTCCGAC TGTTTGAAGA AAAGAGGTAT 
CGAGAAATGA GGAGAAAGAA TATCATTGGT CAAGTTTGTG ATACCCCTAA ATCATATGAT 
AACGTTATGC ATGTAGGTTT GAGGAAGGTG ACCTTCAAGT GGCAAAGAGG AAACAAAATT 
GGGGAGGGGC AGTATGGGAA GGTCTACACC TGTATCAGTG TTGACACTGG CGAATTGATG 
GCTATGAAGG AGATCCGATT TCAACCTAAT GACCATAAAA CTATCAAGGA AACTGCAGAC 
GAATTGAAAA TCTTTGAGGG CATCAAACAC CCCAATCTGG TTCGGTATTT TGGTGTGGAG 
CTTCATAGAG AAGAAATGTA CATCTTCATG GAATACTGTG ACGAGGGGAC ATTAGAAGAG 
GTGTCAAGGC TGGGACTTCA GGAGCATGTC ATTAGGCTGT ATTCAAAGCA GATCACCATC 
GCAATCAACG TCCTCCATGA GCATGGCATC GTTCACCGTG ACATCAAAGG TGCCAATATC 
TTCCTAACCT CCTCTGGACT CATCAAGCTG GGGGATTTTG GATGCTCAGT AAAGCTTAAA 
AACAACGCAC AGACCATGCC GGGAGAAGTG AATAGCACAC TGGGGACAGC AGCATACATG 
GCTCCAGAAG TCATCACTCG TGCAAAAGGA GAAGGCCATG GGCGTGCCGC TGATATCTGG 
AGTCTGGGCT GTGTCGTCAT AGAGATGGTG ACCGGCAAGA GGCCTTGGCA TGAATATGAA 
CACAACTTTC AGATTATGTA TAAAGTGGGA ATGGGGCACA AGCCACCCAT CCCTGAGAGA 
CTAAGCCCGG AAGGAAAGGA CTTCCTTTCT CACTGCCTTG AAAGTGAGCC CAGGATGAGG 
TGGACGGCCA GCCAGCTCCT CGACCATTCC TTCGTCAAGG TCTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAP3K4
Entrez Gene ID	476262
Full Name	mitogen-activated protein kinase kinase kinase 4
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 4 Names mitogen-activated protein kinase kinase kinase 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Mus musculus (house mouse)	Map3k4	NP_036078.2
Gallus gallus (chicken)	MAP3K4	XP_004935618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002371	XP_312585.5
	MAP3K4	XP_001098969.2
Canis lupus familiaris (dog)	MAP3K4	XP_005615756.1
Danio rerio (zebrafish)	map3k4	XP_005157074.1
Xenopus tropicalis (tropical clawed frog)	map3k4	XP_004914705.1
Pan troglodytes (chimpanzee)	MAP3K4	XP_003311625.2
Bos taurus (cattle)	MAP3K4	XP_002690423.2
Rattus norvegicus (Norway rat)	Map3k4	NP_001100926.1
Drosophila melanogaster (fruit fly)	Mekk1	NP_650750.4
Homo sapiens (human)	MAP3K4	NP_005913.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAP3K4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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