AKAP12 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKAP12
Entrez Gene ID	476246
Full Name	A-kinase anchoring protein 12
General protein information	Preferred Names A-kinase anchoring protein 12 Names A-kinase anchor protein 12 A kinase (PRKA) anchor protein (gravin) 12
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005615511.3	XP_005615568.1	A-kinase anchor protein 12 isoform X3
XM_533451.5	XP_533451.2	A-kinase anchor protein 12 isoform X3
XM_005615511.2	XP_005615568.1	A-kinase anchor protein 12 isoform X1
XM_005615512.3	XP_005615569.1	A-kinase anchor protein 12 isoform X2
XM_005615512.2	XP_005615569.1	A-kinase anchor protein 12 isoform X2
XM_533451.6	XP_533451.2	A-kinase anchor protein 12 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	AKAP12	NP_005091.2
Canis lupus familiaris (dog)	AKAP12	XP_533451.2
	AKAP12	XP_002803905.1
Pan troglodytes (chimpanzee)	AKAP12	XP_003311589.1
Bos taurus (cattle)	AKAP12	XP_002690384.2
Mus musculus (house mouse)	Akap12	NP_112462.1
Rattus norvegicus (Norway rat)	Akap12	NP_476444.2

Gene Ontology

The following AKAP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00949	XM_005615511.3 Latest version!	Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe00950	XM_005615512.3 Latest version!	Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe00951	XM_533451.6 Latest version!	Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OCe00949	XM_005615511.2	Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe00950	XM_005615512.2	Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe00951	XM_533451.5	Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKAP12 ( XM_005615511.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005615568.1 Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe00949
Clone ID Related Accession (Same CDS sequence)	XM_005615511.3 , XM_005615511.2
Accession Version	XM_005615511.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	A-kinase anchor protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615511.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGT GGAGTAACCT TTGCTTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615568.1
CDS	116..5146
Translation	MPWWSNLCFGQRESEDVSERDSEKDMAAGSVVVQDITKDGQEEMPETIEQIPSSESNLEELIQPTEAQANDVGFKKVFKFVGFKFTVKKDKTEKSDTVQLLTVKKDEGEGVGGADGAGDHQEPSQETGEATAKESELKQSTEKPEETLKSEQSSTQISLQAESGQAAEEGKDEGEEKQKEPTKSPDSPTSPVASETASPFKKFFTQGWAGWRKKTSFRKPKEDELEASEKKKEQEPEKVDTEGQEKTEDSPEKLAAPEQAHPPEPTDSANAARLSAEYEKVELPSEDQVQGSPEEKPAPLATEVFDDKVEIVAEVHVSMTEKTEEQRAEVEEVVEPLPSEKVAETPAEELVKTKDVCAPGGDHTRPSDLSPDDKVTTAHPEGVVSEVDMLSSQERIKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHHQVAAESPDSTDEQKGESSASSPEEPEEITCLEKGIADAHQDGEAEEGAVSDGEKKREGVTPWASFKKMVTPKKRVRRLSESDKEDELDKVKSATLSSTESAASELQEEAKGNGEEQKPEEPKRKVDTSVSWEALICVGSSKKRARKASSSDDEGEPKAVGGDSQKPDDAGKDKETGPDAALAGSQEHDQGPGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEESVTGSGVEHSASDAEPGKEESWVSIKKFIPGRRKKRSDGKPEQTAVEDAGPAEVNEEDSDVPAVVPLSEYDAVEREKIEAQQAQKSEEQPEQKAAVSVSEELSTDLVRGVTVTVLDGARALTNVEERSPSWISASVTEPLDETEDEAKPLTEDVFEGEVLAEETSVTKPLPEGQDAIDDTVVSEAELTSEAVTAAEATGALCAEEAAEASGAEETADMVSAVSQLTDSPDTTEVATPVQEVEGGVPRLEDQERRTQEVLQAVAEKVREESPLPDTRGPEDTTRTTRGVEAEIPEKVEEAEEDSQRQDLKKETEVPLEERAQETKPETLAQGEVIVQATPESLEKVPQVTDSVESSELRTTCQAETLVGATSESILEQAVAPDSAETLTDSETNGSTPVADFEALHVTQPEQIPGMHEGPEVASGAPPQVTEAEAVPAPEEGPPAPSTFPSEEEDKGHSKMEEILERTDKEVSVEAVPTLSKTDVIEEEGQFADRESKDEALVDGPDVTAAPETTISQEETSDLALQDEATEDPEFQKKEDDTEVQSRTPSPSPVGRELVVQVEKEEIEAKPTQATEEELEQKPTVTVSEELSKELVQTVRVAVIDGEKEVTIWEESCPQLVEEAAVCTEVQVQSSEPLPLTAAVVEEKVLGEAVKSVDASETLEPAGAHLAPEEESSTKDEGLTAQPGAVEVPTGTEARPEPGPGTAPGTASAEPDGGTSAAPEGEKTTPQRQTSEEEDGPAACQEVPAREEDLKAENDLSKLQTESSKLVQNVIQTVVGQLGGAEETAADLQTQAQPLTADGQEASSEARPHVETEGGELQASAPDGTHTVDAKEEPPLTAVEQTPSEVSEASEAVSEAPEKITAIEVESSSVHDQQLEEAALPSEEKKETARTEPVPEDCDGAGSEGRIEESLFESQEDKKGDEVDDPESHHSCSPLEDSEAPGDVSQEAPDTDGPTGKKKEGGQEVEFQEAKVHSEPEDEIQTQTRDETQKQEGEPAKSEPTGS

Target ORF information:

RefSeq Version	XM_005615511.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615511.3

ORF Insert Sequence:

ATGCCCTGGT GGAGTAACCT TTGCTTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP12 ( XM_005615511.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005615568.1 Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe00949
Clone ID Related Accession (Same CDS sequence)	XM_005615511.3 , XM_005615511.2
Accession Version	XM_005615511.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	A-kinase anchor protein 12 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGT GGAGTAACCT TTGCTTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615568.1
CDS	85..5115
Translation	MPWWSNLCFGQRESEDVSERDSEKDMAAGSVVVQDITKDGQEEMPETIEQIPSSESNLEELIQPTEAQANDVGFKKVFKFVGFKFTVKKDKTEKSDTVQLLTVKKDEGEGVGGADGAGDHQEPSQETGEATAKESELKQSTEKPEETLKSEQSSTQISLQAESGQAAEEGKDEGEEKQKEPTKSPDSPTSPVASETASPFKKFFTQGWAGWRKKTSFRKPKEDELEASEKKKEQEPEKVDTEGQEKTEDSPEKLAAPEQAHPPEPTDSANAARLSAEYEKVELPSEDQVQGSPEEKPAPLATEVFDDKVEIVAEVHVSMTEKTEEQRAEVEEVVEPLPSEKVAETPAEELVKTKDVCAPGGDHTRPSDLSPDDKVTTAHPEGVVSEVDMLSSQERIKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHHQVAAESPDSTDEQKGESSASSPEEPEEITCLEKGIADAHQDGEAEEGAVSDGEKKREGVTPWASFKKMVTPKKRVRRLSESDKEDELDKVKSATLSSTESAASELQEEAKGNGEEQKPEEPKRKVDTSVSWEALICVGSSKKRARKASSSDDEGEPKAVGGDSQKPDDAGKDKETGPDAALAGSQEHDQGPGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEESVTGSGVEHSASDAEPGKEESWVSIKKFIPGRRKKRSDGKPEQTAVEDAGPAEVNEEDSDVPAVVPLSEYDAVEREKIEAQQAQKSEEQPEQKAAVSVSEELSTDLVRGVTVTVLDGARALTNVEERSPSWISASVTEPLDETEDEAKPLTEDVFEGEVLAEETSVTKPLPEGQDAIDDTVVSEAELTSEAVTAAEATGALCAEEAAEASGAEETADMVSAVSQLTDSPDTTEVATPVQEVEGGVPRLEDQERRTQEVLQAVAEKVREESPLPDTRGPEDTTRTTRGVEAEIPEKVEEAEEDSQRQDLKKETEVPLEERAQETKPETLAQGEVIVQATPESLEKVPQVTDSVESSELRTTCQAETLVGATSESILEQAVAPDSAETLTDSETNGSTPVADFEALHVTQPEQIPGMHEGPEVASGAPPQVTEAEAVPAPEEGPPAPSTFPSEEEDKGHSKMEEILERTDKEVSVEAVPTLSKTDVIEEEGQFADRESKDEALVDGPDVTAAPETTISQEETSDLALQDEATEDPEFQKKEDDTEVQSRTPSPSPVGRELVVQVEKEEIEAKPTQATEEELEQKPTVTVSEELSKELVQTVRVAVIDGEKEVTIWEESCPQLVEEAAVCTEVQVQSSEPLPLTAAVVEEKVLGEAVKSVDASETLEPAGAHLAPEEESSTKDEGLTAQPGAVEVPTGTEARPEPGPGTAPGTASAEPDGGTSAAPEGEKTTPQRQTSEEEDGPAACQEVPAREEDLKAENDLSKLQTESSKLVQNVIQTVVGQLGGAEETAADLQTQAQPLTADGQEASSEARPHVETEGGELQASAPDGTHTVDAKEEPPLTAVEQTPSEVSEASEAVSEAPEKITAIEVESSSVHDQQLEEAALPSEEKKETARTEPVPEDCDGAGSEGRIEESLFESQEDKKGDEVDDPESHHSCSPLEDSEAPGDVSQEAPDTDGPTGKKKEGGQEVEFQEAKVHSEPEDEIQTQTRDETQKQEGEPAKSEPTGS

Target ORF information:

RefSeq Version	XM_005615511.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615511.2

ORF Insert Sequence:

ATGCCCTGGT GGAGTAACCT TTGCTTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP12 ( XM_005615512.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005615569.1 Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X2, mRNA.

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CloneID	OCe00950
Clone ID Related Accession (Same CDS sequence)	XM_005615512.3 , XM_005615512.2
Accession Version	XM_005615512.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	A-kinase anchor protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615512.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGA TCATCACTAT CACAGTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615569.1
CDS	159..5189
Translation	MLGIITITVGQRESEDVSERDSEKDMAAGSVVVQDITKDGQEEMPETIEQIPSSESNLEELIQPTEAQANDVGFKKVFKFVGFKFTVKKDKTEKSDTVQLLTVKKDEGEGVGGADGAGDHQEPSQETGEATAKESELKQSTEKPEETLKSEQSSTQISLQAESGQAAEEGKDEGEEKQKEPTKSPDSPTSPVASETASPFKKFFTQGWAGWRKKTSFRKPKEDELEASEKKKEQEPEKVDTEGQEKTEDSPEKLAAPEQAHPPEPTDSANAARLSAEYEKVELPSEDQVQGSPEEKPAPLATEVFDDKVEIVAEVHVSMTEKTEEQRAEVEEVVEPLPSEKVAETPAEELVKTKDVCAPGGDHTRPSDLSPDDKVTTAHPEGVVSEVDMLSSQERIKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHHQVAAESPDSTDEQKGESSASSPEEPEEITCLEKGIADAHQDGEAEEGAVSDGEKKREGVTPWASFKKMVTPKKRVRRLSESDKEDELDKVKSATLSSTESAASELQEEAKGNGEEQKPEEPKRKVDTSVSWEALICVGSSKKRARKASSSDDEGEPKAVGGDSQKPDDAGKDKETGPDAALAGSQEHDQGPGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEESVTGSGVEHSASDAEPGKEESWVSIKKFIPGRRKKRSDGKPEQTAVEDAGPAEVNEEDSDVPAVVPLSEYDAVEREKIEAQQAQKSEEQPEQKAAVSVSEELSTDLVRGVTVTVLDGARALTNVEERSPSWISASVTEPLDETEDEAKPLTEDVFEGEVLAEETSVTKPLPEGQDAIDDTVVSEAELTSEAVTAAEATGALCAEEAAEASGAEETADMVSAVSQLTDSPDTTEVATPVQEVEGGVPRLEDQERRTQEVLQAVAEKVREESPLPDTRGPEDTTRTTRGVEAEIPEKVEEAEEDSQRQDLKKETEVPLEERAQETKPETLAQGEVIVQATPESLEKVPQVTDSVESSELRTTCQAETLVGATSESILEQAVAPDSAETLTDSETNGSTPVADFEALHVTQPEQIPGMHEGPEVASGAPPQVTEAEAVPAPEEGPPAPSTFPSEEEDKGHSKMEEILERTDKEVSVEAVPTLSKTDVIEEEGQFADRESKDEALVDGPDVTAAPETTISQEETSDLALQDEATEDPEFQKKEDDTEVQSRTPSPSPVGRELVVQVEKEEIEAKPTQATEEELEQKPTVTVSEELSKELVQTVRVAVIDGEKEVTIWEESCPQLVEEAAVCTEVQVQSSEPLPLTAAVVEEKVLGEAVKSVDASETLEPAGAHLAPEEESSTKDEGLTAQPGAVEVPTGTEARPEPGPGTAPGTASAEPDGGTSAAPEGEKTTPQRQTSEEEDGPAACQEVPAREEDLKAENDLSKLQTESSKLVQNVIQTVVGQLGGAEETAADLQTQAQPLTADGQEASSEARPHVETEGGELQASAPDGTHTVDAKEEPPLTAVEQTPSEVSEASEAVSEAPEKITAIEVESSSVHDQQLEEAALPSEEKKETARTEPVPEDCDGAGSEGRIEESLFESQEDKKGDEVDDPESHHSCSPLEDSEAPGDVSQEAPDTDGPTGKKKEGGQEVEFQEAKVHSEPEDEIQTQTRDETQKQEGEPAKSEPTGS

Target ORF information:

RefSeq Version	XM_005615512.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615512.3

ORF Insert Sequence:

ATGCTGGGGA TCATCACTAT CACAGTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP12 ( XM_005615512.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005615569.1 Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X2, mRNA.

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CloneID	OCe00950
Clone ID Related Accession (Same CDS sequence)	XM_005615512.3 , XM_005615512.2
Accession Version	XM_005615512.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	A-kinase anchor protein 12 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGA TCATCACTAT CACAGTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615569.1
CDS	151..5181
Translation	MLGIITITVGQRESEDVSERDSEKDMAAGSVVVQDITKDGQEEMPETIEQIPSSESNLEELIQPTEAQANDVGFKKVFKFVGFKFTVKKDKTEKSDTVQLLTVKKDEGEGVGGADGAGDHQEPSQETGEATAKESELKQSTEKPEETLKSEQSSTQISLQAESGQAAEEGKDEGEEKQKEPTKSPDSPTSPVASETASPFKKFFTQGWAGWRKKTSFRKPKEDELEASEKKKEQEPEKVDTEGQEKTEDSPEKLAAPEQAHPPEPTDSANAARLSAEYEKVELPSEDQVQGSPEEKPAPLATEVFDDKVEIVAEVHVSMTEKTEEQRAEVEEVVEPLPSEKVAETPAEELVKTKDVCAPGGDHTRPSDLSPDDKVTTAHPEGVVSEVDMLSSQERIKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHHQVAAESPDSTDEQKGESSASSPEEPEEITCLEKGIADAHQDGEAEEGAVSDGEKKREGVTPWASFKKMVTPKKRVRRLSESDKEDELDKVKSATLSSTESAASELQEEAKGNGEEQKPEEPKRKVDTSVSWEALICVGSSKKRARKASSSDDEGEPKAVGGDSQKPDDAGKDKETGPDAALAGSQEHDQGPGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEESVTGSGVEHSASDAEPGKEESWVSIKKFIPGRRKKRSDGKPEQTAVEDAGPAEVNEEDSDVPAVVPLSEYDAVEREKIEAQQAQKSEEQPEQKAAVSVSEELSTDLVRGVTVTVLDGARALTNVEERSPSWISASVTEPLDETEDEAKPLTEDVFEGEVLAEETSVTKPLPEGQDAIDDTVVSEAELTSEAVTAAEATGALCAEEAAEASGAEETADMVSAVSQLTDSPDTTEVATPVQEVEGGVPRLEDQERRTQEVLQAVAEKVREESPLPDTRGPEDTTRTTRGVEAEIPEKVEEAEEDSQRQDLKKETEVPLEERAQETKPETLAQGEVIVQATPESLEKVPQVTDSVESSELRTTCQAETLVGATSESILEQAVAPDSAETLTDSETNGSTPVADFEALHVTQPEQIPGMHEGPEVASGAPPQVTEAEAVPAPEEGPPAPSTFPSEEEDKGHSKMEEILERTDKEVSVEAVPTLSKTDVIEEEGQFADRESKDEALVDGPDVTAAPETTISQEETSDLALQDEATEDPEFQKKEDDTEVQSRTPSPSPVGRELVVQVEKEEIEAKPTQATEEELEQKPTVTVSEELSKELVQTVRVAVIDGEKEVTIWEESCPQLVEEAAVCTEVQVQSSEPLPLTAAVVEEKVLGEAVKSVDASETLEPAGAHLAPEEESSTKDEGLTAQPGAVEVPTGTEARPEPGPGTAPGTASAEPDGGTSAAPEGEKTTPQRQTSEEEDGPAACQEVPAREEDLKAENDLSKLQTESSKLVQNVIQTVVGQLGGAEETAADLQTQAQPLTADGQEASSEARPHVETEGGELQASAPDGTHTVDAKEEPPLTAVEQTPSEVSEASEAVSEAPEKITAIEVESSSVHDQQLEEAALPSEEKKETARTEPVPEDCDGAGSEGRIEESLFESQEDKKGDEVDDPESHHSCSPLEDSEAPGDVSQEAPDTDGPTGKKKEGGQEVEFQEAKVHSEPEDEIQTQTRDETQKQEGEPAKSEPTGS

Target ORF information:

RefSeq Version	XM_005615512.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615512.2

ORF Insert Sequence:

ATGCTGGGGA TCATCACTAT CACAGTTGGA CAGAGAGAGT CTGAAGATGT GAGTGAAAGA 
GACTCAGAAA AAGACATGGC TGCTGGTTCC GTAGTTGTTC AGGACATCAC AAAGGATGGG 
CAGGAGGAGA TGCCTGAGAC CATCGAACAG ATTCCTTCTT CAGAAAGCAA TTTAGAAGAG 
CTGATACAAC CTACTGAGGC CCAGGCTAAT GATGTTGGAT TTAAGAAGGT GTTTAAGTTT 
GTCGGCTTTA AATTCACTGT AAAAAAGGAT AAGACCGAGA AGTCTGACAC TGTCCAGCTA 
CTCACTGTCA AAAAAGATGA AGGTGAAGGA GTGGGAGGAG CAGATGGGGC TGGTGACCAC 
CAGGAGCCCA GCCAGGAGAC TGGAGAAGCA ACAGCCAAAG AAAGTGAACT GAAACAATCC 
ACAGAGAAAC CTGAAGAAAC CCTTAAAAGT GAGCAAAGCA GCACACAAAT TTCTCTTCAG 
GCTGAGTCTG GTCAAGCAGC AGAAGAAGGC AAAGATGAAG GCGAAGAAAA GCAGAAAGAA 
CCCACCAAAT CTCCAGACTC TCCCACTAGT CCAGTGGCCA GTGAAACAGC GTCACCCTTC 
AAAAAATTCT TCACTCAAGG TTGGGCCGGC TGGAGAAAAA AGACCAGTTT CAGGAAGCCT 
AAGGAGGATG AGCTGGAAGC TTCAGAGAAG AAAAAGGAGC AAGAGCCAGA AAAAGTAGAC 
ACAGAAGGAC AGGAAAAGAC CGAAGATTCC CCTGAGAAGT TGGCTGCCCC TGAACAGGCG 
CACCCACCGG AGCCCACCGA CAGTGCTAAC GCGGCCAGAT TGTCAGCGGA ATATGAAAAA 
GTGGAGCTGC CCTCTGAAGA TCAAGTGCAG GGATCTCCTG AAGAGAAACC TGCCCCTTTA 
GCAACAGAAG TATTTGATGA TAAAGTAGAG ATTGTTGCCG AAGTCCACGT TAGCATGACA 
GAGAAGACCG AAGAGCAGAG AGCTGAGGTA GAAGAAGTAG TAGAGCCCTT ACCATCTGAA 
AAAGTGGCGG AAACACCTGC TGAGGAGCTG GTGAAGACCA AAGACGTGTG CGCCCCTGGA 
GGGGACCACA CCCGGCCAAG CGACCTAAGC CCCGATGACA AAGTGACGAC TGCACACCCT 
GAGGGCGTCG TGAGTGAAGT GGACATGCTG TCCTCGCAAG AGAGAATAAA GGTGCAAGGA 
AGCCCTTTAA AGAAACTTTT TACTAGCACT GGCTTAAAAA AGCTTTCTGG AAAGAAACAG 
AAAGGGAAAA GAGGAGGAGG GGATGAAGAG TCGGGGGAAC ACCATCAAGT TGCAGCAGAG 
TCTCCAGACA GTACAGATGA ACAGAAGGGC GAGAGCTCCG CTTCATCCCC TGAGGAACCA 
GAGGAGATTA CGTGTCTGGA GAAAGGCATC GCGGACGCCC ACCAGGATGG CGAAGCTGAG 
GAAGGAGCTG TTTCTGATGG GGAAAAGAAG AGAGAAGGTG TTACTCCCTG GGCGTCTTTC 
AAGAAGATGG TGACGCCCAA GAAACGCGTT AGGCGGCTTT CAGAGAGTGA TAAAGAAGAT 
GAATTGGATA AAGTCAAGAG TGCCACCTTG TCTTCCACGG AGAGCGCGGC CTCTGAGTTG 
CAGGAGGAAG CGAAAGGAAA TGGAGAAGAG CAGAAACCAG AAGAACCGAA GCGCAAAGTT 
GACACTTCGG TATCTTGGGA AGCTTTAATT TGTGTGGGAT CATCCAAGAA AAGAGCAAGA 
AAAGCATCAT CTTCTGATGA TGAAGGGGAA CCAAAAGCCG TGGGAGGAGA CAGCCAGAAA 
CCAGATGATG CAGGAAAAGA CAAAGAAACC GGACCAGACG CTGCCCTTGC TGGTTCCCAA 
GAACATGATC AAGGGCCAGG AAGCTCCTCA CCTGAGCAAG CTGGAAGCCC CACCGAAGGG 
GAGGGAGTTT CCACCTGGGA GTCCTTTAAA CGATTAGTCA CTCCAAGGAA AAAATCGAAG 
TCAAAACTGG AAGAGAAAAG TGAAGAGTCT GTAACTGGGT CTGGTGTAGA ACATTCAGCT 
TCAGATGCAG AACCTGGGAA AGAAGAGTCT TGGGTTTCGA TTAAGAAATT CATTCCCGGA 
CGACGGAAGA AAAGGTCCGA TGGGAAACCA GAACAAACCG CTGTGGAAGA CGCAGGACCA 
GCAGAGGTCA ATGAAGAGGA TTCCGATGTC CCGGCCGTGG TTCCCCTGTC CGAGTATGAT 
GCAGTAGAAA GGGAGAAAAT CGAAGCCCAG CAGGCCCAAA AAAGTGAGGA GCAGCCTGAG 
CAGAAGGCCG CTGTTTCTGT GTCAGAGGAG CTCAGTACGG ATCTGGTACG TGGGGTCACG 
GTGACTGTCC TTGACGGGGC AAGGGCTCTT ACCAATGTGG AAGAAAGGTC GCCATCTTGG 
ATATCCGCTT CGGTGACAGA GCCTCTTGAC GAAACGGAAG ATGAAGCCAA ACCCCTAACC 
GAGGACGTGT TTGAAGGAGA AGTCCTTGCA GAGGAAACCT CTGTTACCAA ACCTCTACCA 
GAGGGTCAAG ATGCCATTGA TGACACAGTC GTGAGTGAGG CGGAATTAAC TTCGGAAGCT 
GTGACAGCCG CGGAAGCTAC CGGGGCCCTT TGTGCTGAAG AAGCAGCAGA GGCATCTGGT 
GCTGAAGAGA CCGCCGACAT GGTGTCCGCT GTTTCTCAGC TAACGGACTC TCCAGACACC 
ACAGAGGTAG CAACGCCTGT TCAGGAGGTG GAAGGTGGCG TACCACGCCT GGAAGACCAA 
GAGAGGCGGA CTCAAGAGGT CCTGCAGGCA GTTGCAGAAA AAGTGAGAGA AGAATCACCA 
CTGCCTGACA CCAGAGGGCC AGAAGACACC ACTCGGACAA CGCGGGGAGT AGAAGCCGAG 
ATACCAGAGA AGGTGGAGGA AGCGGAAGAG GATTCACAAA GGCAAGACCT GAAGAAAGAG 
ACGGAGGTAC CGCTGGAAGA ACGTGCACAA GAAACTAAAC CTGAGACTTT GGCACAAGGG 
GAAGTAATCG TACAGGCCAC CCCAGAAAGC TTGGAGAAAG TTCCTCAAGT CACAGACAGC 
GTGGAGTCCA GTGAGCTTAG AACCACTTGT CAAGCTGAAA CCCTGGTTGG GGCAACATCA 
GAATCGATTC TGGAACAGGC TGTTGCCCCG GACTCAGCTG AAACCCTCAC AGACAGCGAG 
ACCAACGGGA GCACCCCAGT AGCTGATTTT GAAGCTCTCC ATGTAACACA ACCAGAGCAG 
ATCCCGGGGA TGCACGAAGG TCCTGAGGTT GCATCGGGCG CACCGCCCCA GGTCACAGAA 
GCAGAGGCAG TTCCTGCACC CGAAGAGGGG CCTCCAGCAC CTTCTACTTT CCCATCCGAA 
GAAGAAGATA AAGGACATTC AAAAATGGAA GAGATTCTAG AACGCACGGA CAAAGAGGTA 
TCGGTGGAAG CTGTACCCAC TCTTTCAAAG ACCGACGTGA TAGAAGAGGA AGGCCAGTTC 
GCCGATAGGG AAAGCAAAGA CGAAGCTCTC GTCGACGGCC CTGATGTGAC AGCGGCCCCT 
GAAACAACCA TCAGTCAGGA AGAGACGAGT GACCTTGCCC TTCAAGATGA AGCTACTGAA 
GACCCCGAAT TTCAGAAGAA GGAGGATGAT ACCGAAGTCC AGAGCCGTAC ACCCTCTCCC 
AGCCCGGTGG GGAGAGAGCT GGTAGTTCAA GTAGAAAAGG AGGAAATAGA AGCAAAGCCA 
ACCCAAGCCA CCGAAGAAGA ACTTGAGCAA AAACCCACCG TGACCGTCTC TGAGGAGCTT 
AGCAAGGAAC TGGTGCAGAC AGTTAGGGTG GCCGTCATCG ACGGGGAAAA GGAGGTTACC 
ATTTGGGAAG AAAGTTGTCC CCAGCTAGTT GAAGAGGCAG CGGTATGCAC AGAAGTCCAA 
GTGCAGAGCT CTGAACCGTT ACCTCTAACA GCCGCAGTGG TGGAGGAGAA GGTCTTGGGA 
GAAGCTGTCA AGAGTGTAGA TGCCAGTGAG ACCCTGGAAC CTGCGGGCGC ACATTTAGCC 
CCAGAAGAAG AATCCTCCAC AAAGGATGAA GGCCTGACGG CCCAGCCAGG GGCGGTCGAG 
GTGCCCACGG GGACTGAGGC TCGGCCGGAA CCTGGACCGG GAACTGCCCC GGGAACCGCA 
TCAGCTGAGC CTGACGGAGG CACGAGCGCT GCCCCGGAAG GAGAGAAAAC CACCCCCCAG 
AGACAGACCT CAGAGGAAGA GGACGGGCCG GCTGCTTGCC AGGAAGTCCC GGCAAGAGAG 
GAGGATCTAA AGGCTGAAAA CGACCTCTCG AAACTTCAGA CCGAGAGTAG TAAACTTGTA 
CAGAATGTGA TCCAGACAGT TGTTGGTCAG TTAGGAGGCG CGGAAGAAAC AGCCGCGGAC 
CTCCAGACCC AGGCTCAGCC GCTGACGGCG GACGGCCAGG AGGCCAGCTC GGAGGCCAGG 
CCGCACGTTG AGACGGAAGG AGGCGAGCTT CAGGCCTCTG CACCAGATGG AACCCACACT 
GTTGACGCCA AAGAAGAACC CCCACTAACC GCCGTGGAGC AGACCCCTTC TGAGGTTTCT 
GAAGCCTCGG AAGCTGTGAG TGAAGCTCCA GAAAAGATCA CAGCCATCGA GGTAGAAAGT 
TCAAGCGTCC ATGACCAGCA GCTTGAAGAG GCAGCTCTCC CATCTGAAGA AAAGAAAGAA 
ACAGCCAGAA CCGAGCCTGT CCCAGAAGAT TGTGATGGTG CCGGGTCAGA AGGAAGGATA 
GAGGAGTCGC TCTTTGAATC CCAAGAAGAT AAAAAAGGTG ATGAGGTTGA TGACCCTGAG 
AGCCACCACT CGTGCTCACC CCTGGAAGAT TCTGAGGCCC CAGGAGACGT AAGCCAAGAG 
GCCCCGGACA CGGATGGACC AACAGGCAAA AAGAAGGAAG GTGGCCAGGA AGTAGAATTC 
CAGGAAGCCA AAGTCCACAG CGAGCCAGAA GATGAGATTC AAACCCAAAC ACGGGACGAG 
ACACAGAAAC AAGAGGGAGA ACCGGCCAAA TCAGAACCCA CAGGATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP12 ( XM_533451.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533451.2 Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X3, mRNA.

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CloneID	OCe00951
Clone ID Related Accession (Same CDS sequence)	XM_533451.5 , XM_533451.6
Accession Version	XM_533451.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	A-kinase anchor protein 12 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533451.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCCG GGAGCTCCAC GGAGCAGCGC AGCCCCGAGC AGCCGGAGGC GGGGAGCACC 
ACGCCGGCCG AGCCCCAGCC CGGCGGCGGC GGCCTTGCGG CGGAGGCGGC TCCCGGCGAG 
CCGGGGGACC CCGCCATCGC CGCCGCGGAC CCCGCCACCA AGCTTCTGCA GAAGAATGGT 
CAGCTGTCTA GTGCCAACGG CTTAGCTGAG GAAGAAGAGT TCAGCCCCCA GGAGGGAGCC 
CTGAACGGCC AGGAGGAAGA AGTCACTGTC ACAGATGTTG GACAGAGAGA GTCTGAAGAT 
GTGAGTGAAA GAGACTCAGA AAAAGACATG GCTGCTGGTT CCGTAGTTGT TCAGGACATC 
ACAAAGGATG GGCAGGAGGA GATGCCTGAG ACCATCGAAC AGATTCCTTC TTCAGAAAGC 
AATTTAGAAG AGCTGATACA ACCTACTGAG GCCCAGGCTA ATGATGTTGG ATTTAAGAAG 
GTGTTTAAGT TTGTCGGCTT TAAATTCACT GTAAAAAAGG ATAAGACCGA GAAGTCTGAC 
ACTGTCCAGC TACTCACTGT CAAAAAAGAT GAAGGTGAAG GAGTGGGAGG AGCAGATGGG 
GCTGGTGACC ACCAGGAGCC CAGCCAGGAG ACTGGAGAAG CAACAGCCAA AGAAAGTGAA 
CTGAAACAAT CCACAGAGAA ACCTGAAGAA ACCCTTAAAA GTGAGCAAAG CAGCACACAA 
ATTTCTCTTC AGGCTGAGTC TGGTCAAGCA GCAGAAGAAG GCAAAGATGA AGGCGAAGAA 
AAGCAGAAAG AACCCACCAA ATCTCCAGAC TCTCCCACTA GTCCAGTGGC CAGTGAAACA 
GCGTCACCCT TCAAAAAATT CTTCACTCAA GGTTGGGCCG GCTGGAGAAA AAAGACCAGT 
TTCAGGAAGC CTAAGGAGGA TGAGCTGGAA GCTTCAGAGA AGAAAAAGGA GCAAGAGCCA 
GAAAAAGTAG ACACAGAAGG ACAGGAAAAG ACCGAAGATT CCCCTGAGAA GTTGGCTGCC 
CCTGAACAGG CGCACCCACC GGAGCCCACC GACAGTGCTA ACGCGGCCAG ATTGTCAGCG 
GAATATGAAA AAGTGGAGCT GCCCTCTGAA GATCAAGTGC AGGGATCTCC TGAAGAGAAA 
CCTGCCCCTT TAGCAACAGA AGTATTTGAT GATAAAGTAG AGATTGTTGC CGAAGTCCAC 
GTTAGCATGA CAGAGAAGAC CGAAGAGCAG AGAGCTGAGG TAGAAGAAGT AGTAGAGCCC 
TTACCATCTG AAAAAGTGGC GGAAACACCT GCTGAGGAGC TGGTGAAGAC CAAAGACGTG 
TGCGCCCCTG GAGGGGACCA CACCCGGCCA AGCGACCTAA GCCCCGATGA CAAAGTGACG 
ACTGCACACC CTGAGGGCGT CGTGAGTGAA GTGGACATGC TGTCCTCGCA AGAGAGAATA 
AAGGTGCAAG GAAGCCCTTT AAAGAAACTT TTTACTAGCA CTGGCTTAAA AAAGCTTTCT 
GGAAAGAAAC AGAAAGGGAA AAGAGGAGGA GGGGATGAAG AGTCGGGGGA ACACCATCAA 
GTTGCAGCAG AGTCTCCAGA CAGTACAGAT GAACAGAAGG GCGAGAGCTC CGCTTCATCC 
CCTGAGGAAC CAGAGGAGAT TACGTGTCTG GAGAAAGGCA TCGCGGACGC CCACCAGGAT 
GGCGAAGCTG AGGAAGGAGC TGTTTCTGAT GGGGAAAAGA AGAGAGAAGG TGTTACTCCC 
TGGGCGTCTT TCAAGAAGAT GGTGACGCCC AAGAAACGCG TTAGGCGGCT TTCAGAGAGT 
GATAAAGAAG ATGAATTGGA TAAAGTCAAG AGTGCCACCT TGTCTTCCAC GGAGAGCGCG 
GCCTCTGAGT TGCAGGAGGA AGCGAAAGGA AATGGAGAAG AGCAGAAACC AGAAGAACCG 
AAGCGCAAAG TTGACACTTC GGTATCTTGG GAAGCTTTAA TTTGTGTGGG ATCATCCAAG 
AAAAGAGCAA GAAAAGCATC ATCTTCTGAT GATGAAGGGG AACCAAAAGC CGTGGGAGGA 
GACAGCCAGA AACCAGATGA TGCAGGAAAA GACAAAGAAA CCGGACCAGA CGCTGCCCTT 
GCTGGTTCCC AAGAACATGA TCAAGGGCCA GGAAGCTCCT CACCTGAGCA AGCTGGAAGC 
CCCACCGAAG GGGAGGGAGT TTCCACCTGG GAGTCCTTTA AACGATTAGT CACTCCAAGG 
AAAAAATCGA AGTCAAAACT GGAAGAGAAA AGTGAAGAGT CTGTAACTGG GTCTGGTGTA 
GAACATTCAG CTTCAGATGC AGAACCTGGG AAAGAAGAGT CTTGGGTTTC GATTAAGAAA 
TTCATTCCCG GACGACGGAA GAAAAGGTCC GATGGGAAAC CAGAACAAAC CGCTGTGGAA 
GACGCAGGAC CAGCAGAGGT CAATGAAGAG GATTCCGATG TCCCGGCCGT GGTTCCCCTG 
TCCGAGTATG ATGCAGTAGA AAGGGAGAAA ATCGAAGCCC AGCAGGCCCA AAAAAGTGAG 
GAGCAGCCTG AGCAGAAGGC CGCTGTTTCT GTGTCAGAGG AGCTCAGTAC GGATCTGGTA 
CGTGGGGTCA CGGTGACTGT CCTTGACGGG GCAAGGGCTC TTACCAATGT GGAAGAAAGG 
TCGCCATCTT GGATATCCGC TTCGGTGACA GAGCCTCTTG ACGAAACGGA AGATGAAGCC 
AAACCCCTAA CCGAGGACGT GTTTGAAGGA GAAGTCCTTG CAGAGGAAAC CTCTGTTACC 
AAACCTCTAC CAGAGGGTCA AGATGCCATT GATGACACAG TCGTGAGTGA GGCGGAATTA 
ACTTCGGAAG CTGTGACAGC CGCGGAAGCT ACCGGGGCCC TTTGTGCTGA AGAAGCAGCA 
GAGGCATCTG GTGCTGAAGA GACCGCCGAC ATGGTGTCCG CTGTTTCTCA GCTAACGGAC 
TCTCCAGACA CCACAGAGGT AGCAACGCCT GTTCAGGAGG TGGAAGGTGG CGTACCACGC 
CTGGAAGACC AAGAGAGGCG GACTCAAGAG GTCCTGCAGG CAGTTGCAGA AAAAGTGAGA 
GAAGAATCAC CACTGCCTGA CACCAGAGGG CCAGAAGACA CCACTCGGAC AACGCGGGGA 
GTAGAAGCCG AGATACCAGA GAAGGTGGAG GAAGCGGAAG AGGATTCACA AAGGCAAGAC 
CTGAAGAAAG AGACGGAGGT ACCGCTGGAA GAACGTGCAC AAGAAACTAA ACCTGAGACT 
TTGGCACAAG GGGAAGTAAT CGTACAGGCC ACCCCAGAAA GCTTGGAGAA AGTTCCTCAA 
GTCACAGACA GCGTGGAGTC CAGTGAGCTT AGAACCACTT GTCAAGCTGA AACCCTGGTT 
GGGGCAACAT CAGAATCGAT TCTGGAACAG GCTGTTGCCC CGGACTCAGC TGAAACCCTC 
ACAGACAGCG AGACCAACGG GAGCACCCCA GTAGCTGATT TTGAAGCTCT CCATGTAACA 
CAACCAGAGC AGATCCCGGG GATGCACGAA GGTCCTGAGG TTGCATCGGG CGCACCGCCC 
CAGGTCACAG AAGCAGAGGC AGTTCCTGCA CCCGAAGAGG GGCCTCCAGC ACCTTCTACT 
TTCCCATCCG AAGAAGAAGA TAAAGGACAT TCAAAAATGG AAGAGATTCT AGAACGCACG 
GACAAAGAGG TATCGGTGGA AGCTGTACCC ACTCTTTCAA AGACCGACGT GATAGAAGAG 
GAAGGCCAGT TCGCCGATAG GGAAAGCAAA GACGAAGCTC TCGTCGACGG CCCTGATGTG 
ACAGCGGCCC CTGAAACAAC CATCAGTCAG GAAGAGACGA GTGACCTTGC CCTTCAAGAT 
GAAGCTACTG AAGACCCCGA ATTTCAGAAG AAGGAGGATG ATACCGAAGT CCAGAGCCGT 
ACACCCTCTC CCAGCCCGGT GGGGAGAGAG CTGGTAGTTC AAGTAGAAAA GGAGGAAATA 
GAAGCAAAGC CAACCCAAGC CACCGAAGAA GAACTTGAGC AAAAACCCAC CGTGACCGTC 
TCTGAGGAGC TTAGCAAGGA ACTGGTGCAG ACAGTTAGGG TGGCCGTCAT CGACGGGGAA 
AAGGAGGTTA CCATTTGGGA AGAAAGTTGT CCCCAGCTAG TTGAAGAGGC AGCGGTATGC 
ACAGAAGTCC AAGTGCAGAG CTCTGAACCG TTACCTCTAA CAGCCGCAGT GGTGGAGGAG 
AAGGTCTTGG GAGAAGCTGT CAAGAGTGTA GATGCCAGTG AGACCCTGGA ACCTGCGGGC 
GCACATTTAG CCCCAGAAGA AGAATCCTCC ACAAAGGATG AAGGCCTGAC GGCCCAGCCA 
GGGGCGGTCG AGGTGCCCAC GGGGACTGAG GCTCGGCCGG AACCTGGACC GGGAACTGCC 
CCGGGAACCG CATCAGCTGA GCCTGACGGA GGCACGAGCG CTGCCCCGGA AGGAGAGAAA 
ACCACCCCCC AGAGACAGAC CTCAGAGGAA GAGGACGGGC CGGCTGCTTG CCAGGAAGTC 
CCGGCAAGAG AGGAGGATCT AAAGGCTGAA AACGACCTCT CGAAACTTCA GACCGAGAGT 
AGTAAACTTG TACAGAATGT GATCCAGACA GTTGTTGGTC AGTTAGGAGG CGCGGAAGAA 
ACAGCCGCGG ACCTCCAGAC CCAGGCTCAG CCGCTGACGG CGGACGGCCA GGAGGCCAGC 
TCGGAGGCCA GGCCGCACGT TGAGACGGAA GGAGGCGAGC TTCAGGCCTC TGCACCAGAT 
GGAACCCACA CTGTTGACGC CAAAGAAGAA CCCCCACTAA CCGCCGTGGA GCAGACCCCT 
TCTGAGGTTT CTGAAGCCTC GGAAGCTGTG AGTGAAGCTC CAGAAAAGAT CACAGCCATC 
GAGGTAGAAA GTTCAAGCGT CCATGACCAG CAGCTTGAAG AGGCAGCTCT CCCATCTGAA 
GAAAAGAAAG AAACAGCCAG AACCGAGCCT GTCCCAGAAG ATTGTGATGG TGCCGGGTCA 
GAAGGAAGGA TAGAGGAGTC GCTCTTTGAA TCCCAAGAAG ATAAAAAAGG TGATGAGGTT 
GATGACCCTG AGAGCCACCA CTCGTGCTCA CCCCTGGAAG ATTCTGAGGC CCCAGGAGAC 
GTAAGCCAAG AGGCCCCGGA CACGGATGGA CCAACAGGCA AAAAGAAGGA AGGTGGCCAG 
GAAGTAGAAT TCCAGGAAGC CAAAGTCCAC AGCGAGCCAG AAGATGAGAT TCAAACCCAA 
ACACGGGACG AGACACAGAA ACAAGAGGGA GAACCGGCCA AATCAGAACC CACAGGATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533451.2
CDS	196..5478
Translation	MGAGSSTEQRSPEQPEAGSTTPAEPQPGGGGLAAEAAPGEPGDPAIAAADPATKLLQKNGQLSSANGLAEEEEFSPQEGALNGQEEEVTVTDVGQRESEDVSERDSEKDMAAGSVVVQDITKDGQEEMPETIEQIPSSESNLEELIQPTEAQANDVGFKKVFKFVGFKFTVKKDKTEKSDTVQLLTVKKDEGEGVGGADGAGDHQEPSQETGEATAKESELKQSTEKPEETLKSEQSSTQISLQAESGQAAEEGKDEGEEKQKEPTKSPDSPTSPVASETASPFKKFFTQGWAGWRKKTSFRKPKEDELEASEKKKEQEPEKVDTEGQEKTEDSPEKLAAPEQAHPPEPTDSANAARLSAEYEKVELPSEDQVQGSPEEKPAPLATEVFDDKVEIVAEVHVSMTEKTEEQRAEVEEVVEPLPSEKVAETPAEELVKTKDVCAPGGDHTRPSDLSPDDKVTTAHPEGVVSEVDMLSSQERIKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHHQVAAESPDSTDEQKGESSASSPEEPEEITCLEKGIADAHQDGEAEEGAVSDGEKKREGVTPWASFKKMVTPKKRVRRLSESDKEDELDKVKSATLSSTESAASELQEEAKGNGEEQKPEEPKRKVDTSVSWEALICVGSSKKRARKASSSDDEGEPKAVGGDSQKPDDAGKDKETGPDAALAGSQEHDQGPGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEESVTGSGVEHSASDAEPGKEESWVSIKKFIPGRRKKRSDGKPEQTAVEDAGPAEVNEEDSDVPAVVPLSEYDAVEREKIEAQQAQKSEEQPEQKAAVSVSEELSTDLVRGVTVTVLDGARALTNVEERSPSWISASVTEPLDETEDEAKPLTEDVFEGEVLAEETSVTKPLPEGQDAIDDTVVSEAELTSEAVTAAEATGALCAEEAAEASGAEETADMVSAVSQLTDSPDTTEVATPVQEVEGGVPRLEDQERRTQEVLQAVAEKVREESPLPDTRGPEDTTRTTRGVEAEIPEKVEEAEEDSQRQDLKKETEVPLEERAQETKPETLAQGEVIVQATPESLEKVPQVTDSVESSELRTTCQAETLVGATSESILEQAVAPDSAETLTDSETNGSTPVADFEALHVTQPEQIPGMHEGPEVASGAPPQVTEAEAVPAPEEGPPAPSTFPSEEEDKGHSKMEEILERTDKEVSVEAVPTLSKTDVIEEEGQFADRESKDEALVDGPDVTAAPETTISQEETSDLALQDEATEDPEFQKKEDDTEVQSRTPSPSPVGRELVVQVEKEEIEAKPTQATEEELEQKPTVTVSEELSKELVQTVRVAVIDGEKEVTIWEESCPQLVEEAAVCTEVQVQSSEPLPLTAAVVEEKVLGEAVKSVDASETLEPAGAHLAPEEESSTKDEGLTAQPGAVEVPTGTEARPEPGPGTAPGTASAEPDGGTSAAPEGEKTTPQRQTSEEEDGPAACQEVPAREEDLKAENDLSKLQTESSKLVQNVIQTVVGQLGGAEETAADLQTQAQPLTADGQEASSEARPHVETEGGELQASAPDGTHTVDAKEEPPLTAVEQTPSEVSEASEAVSEAPEKITAIEVESSSVHDQQLEEAALPSEEKKETARTEPVPEDCDGAGSEGRIEESLFESQEDKKGDEVDDPESHHSCSPLEDSEAPGDVSQEAPDTDGPTGKKKEGGQEVEFQEAKVHSEPEDEIQTQTRDETQKQEGEPAKSEPTGS

Target ORF information:

RefSeq Version	XM_533451.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533451.5

ORF Insert Sequence:

ATGGGTGCCG GGAGCTCCAC GGAGCAGCGC AGCCCCGAGC AGCCGGAGGC GGGGAGCACC 
ACGCCGGCCG AGCCCCAGCC CGGCGGCGGC GGCCTTGCGG CGGAGGCGGC TCCCGGCGAG 
CCGGGGGACC CCGCCATCGC CGCCGCGGAC CCCGCCACCA AGCTTCTGCA GAAGAATGGT 
CAGCTGTCTA GTGCCAACGG CTTAGCTGAG GAAGAAGAGT TCAGCCCCCA GGAGGGAGCC 
CTGAACGGCC AGGAGGAAGA AGTCACTGTC ACAGATGTTG GACAGAGAGA GTCTGAAGAT 
GTGAGTGAAA GAGACTCAGA AAAAGACATG GCTGCTGGTT CCGTAGTTGT TCAGGACATC 
ACAAAGGATG GGCAGGAGGA GATGCCTGAG ACCATCGAAC AGATTCCTTC TTCAGAAAGC 
AATTTAGAAG AGCTGATACA ACCTACTGAG GCCCAGGCTA ATGATGTTGG ATTTAAGAAG 
GTGTTTAAGT TTGTCGGCTT TAAATTCACT GTAAAAAAGG ATAAGACCGA GAAGTCTGAC 
ACTGTCCAGC TACTCACTGT CAAAAAAGAT GAAGGTGAAG GAGTGGGAGG AGCAGATGGG 
GCTGGTGACC ACCAGGAGCC CAGCCAGGAG ACTGGAGAAG CAACAGCCAA AGAAAGTGAA 
CTGAAACAAT CCACAGAGAA ACCTGAAGAA ACCCTTAAAA GTGAGCAAAG CAGCACACAA 
ATTTCTCTTC AGGCTGAGTC TGGTCAAGCA GCAGAAGAAG GCAAAGATGA AGGCGAAGAA 
AAGCAGAAAG AACCCACCAA ATCTCCAGAC TCTCCCACTA GTCCAGTGGC CAGTGAAACA 
GCGTCACCCT TCAAAAAATT CTTCACTCAA GGTTGGGCCG GCTGGAGAAA AAAGACCAGT 
TTCAGGAAGC CTAAGGAGGA TGAGCTGGAA GCTTCAGAGA AGAAAAAGGA GCAAGAGCCA 
GAAAAAGTAG ACACAGAAGG ACAGGAAAAG ACCGAAGATT CCCCTGAGAA GTTGGCTGCC 
CCTGAACAGG CGCACCCACC GGAGCCCACC GACAGTGCTA ACGCGGCCAG ATTGTCAGCG 
GAATATGAAA AAGTGGAGCT GCCCTCTGAA GATCAAGTGC AGGGATCTCC TGAAGAGAAA 
CCTGCCCCTT TAGCAACAGA AGTATTTGAT GATAAAGTAG AGATTGTTGC CGAAGTCCAC 
GTTAGCATGA CAGAGAAGAC CGAAGAGCAG AGAGCTGAGG TAGAAGAAGT AGTAGAGCCC 
TTACCATCTG AAAAAGTGGC GGAAACACCT GCTGAGGAGC TGGTGAAGAC CAAAGACGTG 
TGCGCCCCTG GAGGGGACCA CACCCGGCCA AGCGACCTAA GCCCCGATGA CAAAGTGACG 
ACTGCACACC CTGAGGGCGT CGTGAGTGAA GTGGACATGC TGTCCTCGCA AGAGAGAATA 
AAGGTGCAAG GAAGCCCTTT AAAGAAACTT TTTACTAGCA CTGGCTTAAA AAAGCTTTCT 
GGAAAGAAAC AGAAAGGGAA AAGAGGAGGA GGGGATGAAG AGTCGGGGGA ACACCATCAA 
GTTGCAGCAG AGTCTCCAGA CAGTACAGAT GAACAGAAGG GCGAGAGCTC CGCTTCATCC 
CCTGAGGAAC CAGAGGAGAT TACGTGTCTG GAGAAAGGCA TCGCGGACGC CCACCAGGAT 
GGCGAAGCTG AGGAAGGAGC TGTTTCTGAT GGGGAAAAGA AGAGAGAAGG TGTTACTCCC 
TGGGCGTCTT TCAAGAAGAT GGTGACGCCC AAGAAACGCG TTAGGCGGCT TTCAGAGAGT 
GATAAAGAAG ATGAATTGGA TAAAGTCAAG AGTGCCACCT TGTCTTCCAC GGAGAGCGCG 
GCCTCTGAGT TGCAGGAGGA AGCGAAAGGA AATGGAGAAG AGCAGAAACC AGAAGAACCG 
AAGCGCAAAG TTGACACTTC GGTATCTTGG GAAGCTTTAA TTTGTGTGGG ATCATCCAAG 
AAAAGAGCAA GAAAAGCATC ATCTTCTGAT GATGAAGGGG AACCAAAAGC CGTGGGAGGA 
GACAGCCAGA AACCAGATGA TGCAGGAAAA GACAAAGAAA CCGGACCAGA CGCTGCCCTT 
GCTGGTTCCC AAGAACATGA TCAAGGGCCA GGAAGCTCCT CACCTGAGCA AGCTGGAAGC 
CCCACCGAAG GGGAGGGAGT TTCCACCTGG GAGTCCTTTA AACGATTAGT CACTCCAAGG 
AAAAAATCGA AGTCAAAACT GGAAGAGAAA AGTGAAGAGT CTGTAACTGG GTCTGGTGTA 
GAACATTCAG CTTCAGATGC AGAACCTGGG AAAGAAGAGT CTTGGGTTTC GATTAAGAAA 
TTCATTCCCG GACGACGGAA GAAAAGGTCC GATGGGAAAC CAGAACAAAC CGCTGTGGAA 
GACGCAGGAC CAGCAGAGGT CAATGAAGAG GATTCCGATG TCCCGGCCGT GGTTCCCCTG 
TCCGAGTATG ATGCAGTAGA AAGGGAGAAA ATCGAAGCCC AGCAGGCCCA AAAAAGTGAG 
GAGCAGCCTG AGCAGAAGGC CGCTGTTTCT GTGTCAGAGG AGCTCAGTAC GGATCTGGTA 
CGTGGGGTCA CGGTGACTGT CCTTGACGGG GCAAGGGCTC TTACCAATGT GGAAGAAAGG 
TCGCCATCTT GGATATCCGC TTCGGTGACA GAGCCTCTTG ACGAAACGGA AGATGAAGCC 
AAACCCCTAA CCGAGGACGT GTTTGAAGGA GAAGTCCTTG CAGAGGAAAC CTCTGTTACC 
AAACCTCTAC CAGAGGGTCA AGATGCCATT GATGACACAG TCGTGAGTGA GGCGGAATTA 
ACTTCGGAAG CTGTGACAGC CGCGGAAGCT ACCGGGGCCC TTTGTGCTGA AGAAGCAGCA 
GAGGCATCTG GTGCTGAAGA GACCGCCGAC ATGGTGTCCG CTGTTTCTCA GCTAACGGAC 
TCTCCAGACA CCACAGAGGT AGCAACGCCT GTTCAGGAGG TGGAAGGTGG CGTACCACGC 
CTGGAAGACC AAGAGAGGCG GACTCAAGAG GTCCTGCAGG CAGTTGCAGA AAAAGTGAGA 
GAAGAATCAC CACTGCCTGA CACCAGAGGG CCAGAAGACA CCACTCGGAC AACGCGGGGA 
GTAGAAGCCG AGATACCAGA GAAGGTGGAG GAAGCGGAAG AGGATTCACA AAGGCAAGAC 
CTGAAGAAAG AGACGGAGGT ACCGCTGGAA GAACGTGCAC AAGAAACTAA ACCTGAGACT 
TTGGCACAAG GGGAAGTAAT CGTACAGGCC ACCCCAGAAA GCTTGGAGAA AGTTCCTCAA 
GTCACAGACA GCGTGGAGTC CAGTGAGCTT AGAACCACTT GTCAAGCTGA AACCCTGGTT 
GGGGCAACAT CAGAATCGAT TCTGGAACAG GCTGTTGCCC CGGACTCAGC TGAAACCCTC 
ACAGACAGCG AGACCAACGG GAGCACCCCA GTAGCTGATT TTGAAGCTCT CCATGTAACA 
CAACCAGAGC AGATCCCGGG GATGCACGAA GGTCCTGAGG TTGCATCGGG CGCACCGCCC 
CAGGTCACAG AAGCAGAGGC AGTTCCTGCA CCCGAAGAGG GGCCTCCAGC ACCTTCTACT 
TTCCCATCCG AAGAAGAAGA TAAAGGACAT TCAAAAATGG AAGAGATTCT AGAACGCACG 
GACAAAGAGG TATCGGTGGA AGCTGTACCC ACTCTTTCAA AGACCGACGT GATAGAAGAG 
GAAGGCCAGT TCGCCGATAG GGAAAGCAAA GACGAAGCTC TCGTCGACGG CCCTGATGTG 
ACAGCGGCCC CTGAAACAAC CATCAGTCAG GAAGAGACGA GTGACCTTGC CCTTCAAGAT 
GAAGCTACTG AAGACCCCGA ATTTCAGAAG AAGGAGGATG ATACCGAAGT CCAGAGCCGT 
ACACCCTCTC CCAGCCCGGT GGGGAGAGAG CTGGTAGTTC AAGTAGAAAA GGAGGAAATA 
GAAGCAAAGC CAACCCAAGC CACCGAAGAA GAACTTGAGC AAAAACCCAC CGTGACCGTC 
TCTGAGGAGC TTAGCAAGGA ACTGGTGCAG ACAGTTAGGG TGGCCGTCAT CGACGGGGAA 
AAGGAGGTTA CCATTTGGGA AGAAAGTTGT CCCCAGCTAG TTGAAGAGGC AGCGGTATGC 
ACAGAAGTCC AAGTGCAGAG CTCTGAACCG TTACCTCTAA CAGCCGCAGT GGTGGAGGAG 
AAGGTCTTGG GAGAAGCTGT CAAGAGTGTA GATGCCAGTG AGACCCTGGA ACCTGCGGGC 
GCACATTTAG CCCCAGAAGA AGAATCCTCC ACAAAGGATG AAGGCCTGAC GGCCCAGCCA 
GGGGCGGTCG AGGTGCCCAC GGGGACTGAG GCTCGGCCGG AACCTGGACC GGGAACTGCC 
CCGGGAACCG CATCAGCTGA GCCTGACGGA GGCACGAGCG CTGCCCCGGA AGGAGAGAAA 
ACCACCCCCC AGAGACAGAC CTCAGAGGAA GAGGACGGGC CGGCTGCTTG CCAGGAAGTC 
CCGGCAAGAG AGGAGGATCT AAAGGCTGAA AACGACCTCT CGAAACTTCA GACCGAGAGT 
AGTAAACTTG TACAGAATGT GATCCAGACA GTTGTTGGTC AGTTAGGAGG CGCGGAAGAA 
ACAGCCGCGG ACCTCCAGAC CCAGGCTCAG CCGCTGACGG CGGACGGCCA GGAGGCCAGC 
TCGGAGGCCA GGCCGCACGT TGAGACGGAA GGAGGCGAGC TTCAGGCCTC TGCACCAGAT 
GGAACCCACA CTGTTGACGC CAAAGAAGAA CCCCCACTAA CCGCCGTGGA GCAGACCCCT 
TCTGAGGTTT CTGAAGCCTC GGAAGCTGTG AGTGAAGCTC CAGAAAAGAT CACAGCCATC 
GAGGTAGAAA GTTCAAGCGT CCATGACCAG CAGCTTGAAG AGGCAGCTCT CCCATCTGAA 
GAAAAGAAAG AAACAGCCAG AACCGAGCCT GTCCCAGAAG ATTGTGATGG TGCCGGGTCA 
GAAGGAAGGA TAGAGGAGTC GCTCTTTGAA TCCCAAGAAG ATAAAAAAGG TGATGAGGTT 
GATGACCCTG AGAGCCACCA CTCGTGCTCA CCCCTGGAAG ATTCTGAGGC CCCAGGAGAC 
GTAAGCCAAG AGGCCCCGGA CACGGATGGA CCAACAGGCA AAAAGAAGGA AGGTGGCCAG 
GAAGTAGAAT TCCAGGAAGC CAAAGTCCAC AGCGAGCCAG AAGATGAGAT TCAAACCCAA 
ACACGGGACG AGACACAGAA ACAAGAGGGA GAACCGGCCA AATCAGAACC CACAGGATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP12 ( XM_533451.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533451.2 Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X1, mRNA.

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CloneID	OCe00951
Clone ID Related Accession (Same CDS sequence)	XM_533451.5 , XM_533451.6
Accession Version	XM_533451.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	A-kinase anchor protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533451.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCCG GGAGCTCCAC GGAGCAGCGC AGCCCCGAGC AGCCGGAGGC GGGGAGCACC 
ACGCCGGCCG AGCCCCAGCC CGGCGGCGGC GGCCTTGCGG CGGAGGCGGC TCCCGGCGAG 
CCGGGGGACC CCGCCATCGC CGCCGCGGAC CCCGCCACCA AGCTTCTGCA GAAGAATGGT 
CAGCTGTCTA GTGCCAACGG CTTAGCTGAG GAAGAAGAGT TCAGCCCCCA GGAGGGAGCC 
CTGAACGGCC AGGAGGAAGA AGTCACTGTC ACAGATGTTG GACAGAGAGA GTCTGAAGAT 
GTGAGTGAAA GAGACTCAGA AAAAGACATG GCTGCTGGTT CCGTAGTTGT TCAGGACATC 
ACAAAGGATG GGCAGGAGGA GATGCCTGAG ACCATCGAAC AGATTCCTTC TTCAGAAAGC 
AATTTAGAAG AGCTGATACA ACCTACTGAG GCCCAGGCTA ATGATGTTGG ATTTAAGAAG 
GTGTTTAAGT TTGTCGGCTT TAAATTCACT GTAAAAAAGG ATAAGACCGA GAAGTCTGAC 
ACTGTCCAGC TACTCACTGT CAAAAAAGAT GAAGGTGAAG GAGTGGGAGG AGCAGATGGG 
GCTGGTGACC ACCAGGAGCC CAGCCAGGAG ACTGGAGAAG CAACAGCCAA AGAAAGTGAA 
CTGAAACAAT CCACAGAGAA ACCTGAAGAA ACCCTTAAAA GTGAGCAAAG CAGCACACAA 
ATTTCTCTTC AGGCTGAGTC TGGTCAAGCA GCAGAAGAAG GCAAAGATGA AGGCGAAGAA 
AAGCAGAAAG AACCCACCAA ATCTCCAGAC TCTCCCACTA GTCCAGTGGC CAGTGAAACA 
GCGTCACCCT TCAAAAAATT CTTCACTCAA GGTTGGGCCG GCTGGAGAAA AAAGACCAGT 
TTCAGGAAGC CTAAGGAGGA TGAGCTGGAA GCTTCAGAGA AGAAAAAGGA GCAAGAGCCA 
GAAAAAGTAG ACACAGAAGG ACAGGAAAAG ACCGAAGATT CCCCTGAGAA GTTGGCTGCC 
CCTGAACAGG CGCACCCACC GGAGCCCACC GACAGTGCTA ACGCGGCCAG ATTGTCAGCG 
GAATATGAAA AAGTGGAGCT GCCCTCTGAA GATCAAGTGC AGGGATCTCC TGAAGAGAAA 
CCTGCCCCTT TAGCAACAGA AGTATTTGAT GATAAAGTAG AGATTGTTGC CGAAGTCCAC 
GTTAGCATGA CAGAGAAGAC CGAAGAGCAG AGAGCTGAGG TAGAAGAAGT AGTAGAGCCC 
TTACCATCTG AAAAAGTGGC GGAAACACCT GCTGAGGAGC TGGTGAAGAC CAAAGACGTG 
TGCGCCCCTG GAGGGGACCA CACCCGGCCA AGCGACCTAA GCCCCGATGA CAAAGTGACG 
ACTGCACACC CTGAGGGCGT CGTGAGTGAA GTGGACATGC TGTCCTCGCA AGAGAGAATA 
AAGGTGCAAG GAAGCCCTTT AAAGAAACTT TTTACTAGCA CTGGCTTAAA AAAGCTTTCT 
GGAAAGAAAC AGAAAGGGAA AAGAGGAGGA GGGGATGAAG AGTCGGGGGA ACACCATCAA 
GTTGCAGCAG AGTCTCCAGA CAGTACAGAT GAACAGAAGG GCGAGAGCTC CGCTTCATCC 
CCTGAGGAAC CAGAGGAGAT TACGTGTCTG GAGAAAGGCA TCGCGGACGC CCACCAGGAT 
GGCGAAGCTG AGGAAGGAGC TGTTTCTGAT GGGGAAAAGA AGAGAGAAGG TGTTACTCCC 
TGGGCGTCTT TCAAGAAGAT GGTGACGCCC AAGAAACGCG TTAGGCGGCT TTCAGAGAGT 
GATAAAGAAG ATGAATTGGA TAAAGTCAAG AGTGCCACCT TGTCTTCCAC GGAGAGCGCG 
GCCTCTGAGT TGCAGGAGGA AGCGAAAGGA AATGGAGAAG AGCAGAAACC AGAAGAACCG 
AAGCGCAAAG TTGACACTTC GGTATCTTGG GAAGCTTTAA TTTGTGTGGG ATCATCCAAG 
AAAAGAGCAA GAAAAGCATC ATCTTCTGAT GATGAAGGGG AACCAAAAGC CGTGGGAGGA 
GACAGCCAGA AACCAGATGA TGCAGGAAAA GACAAAGAAA CCGGACCAGA CGCTGCCCTT 
GCTGGTTCCC AAGAACATGA TCAAGGGCCA GGAAGCTCCT CACCTGAGCA AGCTGGAAGC 
CCCACCGAAG GGGAGGGAGT TTCCACCTGG GAGTCCTTTA AACGATTAGT CACTCCAAGG 
AAAAAATCGA AGTCAAAACT GGAAGAGAAA AGTGAAGAGT CTGTAACTGG GTCTGGTGTA 
GAACATTCAG CTTCAGATGC AGAACCTGGG AAAGAAGAGT CTTGGGTTTC GATTAAGAAA 
TTCATTCCCG GACGACGGAA GAAAAGGTCC GATGGGAAAC CAGAACAAAC CGCTGTGGAA 
GACGCAGGAC CAGCAGAGGT CAATGAAGAG GATTCCGATG TCCCGGCCGT GGTTCCCCTG 
TCCGAGTATG ATGCAGTAGA AAGGGAGAAA ATCGAAGCCC AGCAGGCCCA AAAAAGTGAG 
GAGCAGCCTG AGCAGAAGGC CGCTGTTTCT GTGTCAGAGG AGCTCAGTAC GGATCTGGTA 
CGTGGGGTCA CGGTGACTGT CCTTGACGGG GCAAGGGCTC TTACCAATGT GGAAGAAAGG 
TCGCCATCTT GGATATCCGC TTCGGTGACA GAGCCTCTTG ACGAAACGGA AGATGAAGCC 
AAACCCCTAA CCGAGGACGT GTTTGAAGGA GAAGTCCTTG CAGAGGAAAC CTCTGTTACC 
AAACCTCTAC CAGAGGGTCA AGATGCCATT GATGACACAG TCGTGAGTGA GGCGGAATTA 
ACTTCGGAAG CTGTGACAGC CGCGGAAGCT ACCGGGGCCC TTTGTGCTGA AGAAGCAGCA 
GAGGCATCTG GTGCTGAAGA GACCGCCGAC ATGGTGTCCG CTGTTTCTCA GCTAACGGAC 
TCTCCAGACA CCACAGAGGT AGCAACGCCT GTTCAGGAGG TGGAAGGTGG CGTACCACGC 
CTGGAAGACC AAGAGAGGCG GACTCAAGAG GTCCTGCAGG CAGTTGCAGA AAAAGTGAGA 
GAAGAATCAC CACTGCCTGA CACCAGAGGG CCAGAAGACA CCACTCGGAC AACGCGGGGA 
GTAGAAGCCG AGATACCAGA GAAGGTGGAG GAAGCGGAAG AGGATTCACA AAGGCAAGAC 
CTGAAGAAAG AGACGGAGGT ACCGCTGGAA GAACGTGCAC AAGAAACTAA ACCTGAGACT 
TTGGCACAAG GGGAAGTAAT CGTACAGGCC ACCCCAGAAA GCTTGGAGAA AGTTCCTCAA 
GTCACAGACA GCGTGGAGTC CAGTGAGCTT AGAACCACTT GTCAAGCTGA AACCCTGGTT 
GGGGCAACAT CAGAATCGAT TCTGGAACAG GCTGTTGCCC CGGACTCAGC TGAAACCCTC 
ACAGACAGCG AGACCAACGG GAGCACCCCA GTAGCTGATT TTGAAGCTCT CCATGTAACA 
CAACCAGAGC AGATCCCGGG GATGCACGAA GGTCCTGAGG TTGCATCGGG CGCACCGCCC 
CAGGTCACAG AAGCAGAGGC AGTTCCTGCA CCCGAAGAGG GGCCTCCAGC ACCTTCTACT 
TTCCCATCCG AAGAAGAAGA TAAAGGACAT TCAAAAATGG AAGAGATTCT AGAACGCACG 
GACAAAGAGG TATCGGTGGA AGCTGTACCC ACTCTTTCAA AGACCGACGT GATAGAAGAG 
GAAGGCCAGT TCGCCGATAG GGAAAGCAAA GACGAAGCTC TCGTCGACGG CCCTGATGTG 
ACAGCGGCCC CTGAAACAAC CATCAGTCAG GAAGAGACGA GTGACCTTGC CCTTCAAGAT 
GAAGCTACTG AAGACCCCGA ATTTCAGAAG AAGGAGGATG ATACCGAAGT CCAGAGCCGT 
ACACCCTCTC CCAGCCCGGT GGGGAGAGAG CTGGTAGTTC AAGTAGAAAA GGAGGAAATA 
GAAGCAAAGC CAACCCAAGC CACCGAAGAA GAACTTGAGC AAAAACCCAC CGTGACCGTC 
TCTGAGGAGC TTAGCAAGGA ACTGGTGCAG ACAGTTAGGG TGGCCGTCAT CGACGGGGAA 
AAGGAGGTTA CCATTTGGGA AGAAAGTTGT CCCCAGCTAG TTGAAGAGGC AGCGGTATGC 
ACAGAAGTCC AAGTGCAGAG CTCTGAACCG TTACCTCTAA CAGCCGCAGT GGTGGAGGAG 
AAGGTCTTGG GAGAAGCTGT CAAGAGTGTA GATGCCAGTG AGACCCTGGA ACCTGCGGGC 
GCACATTTAG CCCCAGAAGA AGAATCCTCC ACAAAGGATG AAGGCCTGAC GGCCCAGCCA 
GGGGCGGTCG AGGTGCCCAC GGGGACTGAG GCTCGGCCGG AACCTGGACC GGGAACTGCC 
CCGGGAACCG CATCAGCTGA GCCTGACGGA GGCACGAGCG CTGCCCCGGA AGGAGAGAAA 
ACCACCCCCC AGAGACAGAC CTCAGAGGAA GAGGACGGGC CGGCTGCTTG CCAGGAAGTC 
CCGGCAAGAG AGGAGGATCT AAAGGCTGAA AACGACCTCT CGAAACTTCA GACCGAGAGT 
AGTAAACTTG TACAGAATGT GATCCAGACA GTTGTTGGTC AGTTAGGAGG CGCGGAAGAA 
ACAGCCGCGG ACCTCCAGAC CCAGGCTCAG CCGCTGACGG CGGACGGCCA GGAGGCCAGC 
TCGGAGGCCA GGCCGCACGT TGAGACGGAA GGAGGCGAGC TTCAGGCCTC TGCACCAGAT 
GGAACCCACA CTGTTGACGC CAAAGAAGAA CCCCCACTAA CCGCCGTGGA GCAGACCCCT 
TCTGAGGTTT CTGAAGCCTC GGAAGCTGTG AGTGAAGCTC CAGAAAAGAT CACAGCCATC 
GAGGTAGAAA GTTCAAGCGT CCATGACCAG CAGCTTGAAG AGGCAGCTCT CCCATCTGAA 
GAAAAGAAAG AAACAGCCAG AACCGAGCCT GTCCCAGAAG ATTGTGATGG TGCCGGGTCA 
GAAGGAAGGA TAGAGGAGTC GCTCTTTGAA TCCCAAGAAG ATAAAAAAGG TGATGAGGTT 
GATGACCCTG AGAGCCACCA CTCGTGCTCA CCCCTGGAAG ATTCTGAGGC CCCAGGAGAC 
GTAAGCCAAG AGGCCCCGGA CACGGATGGA CCAACAGGCA AAAAGAAGGA AGGTGGCCAG 
GAAGTAGAAT TCCAGGAAGC CAAAGTCCAC AGCGAGCCAG AAGATGAGAT TCAAACCCAA 
ACACGGGACG AGACACAGAA ACAAGAGGGA GAACCGGCCA AATCAGAACC CACAGGATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533451.2
CDS	16..5298
Translation	MGAGSSTEQRSPEQPEAGSTTPAEPQPGGGGLAAEAAPGEPGDPAIAAADPATKLLQKNGQLSSANGLAEEEEFSPQEGALNGQEEEVTVTDVGQRESEDVSERDSEKDMAAGSVVVQDITKDGQEEMPETIEQIPSSESNLEELIQPTEAQANDVGFKKVFKFVGFKFTVKKDKTEKSDTVQLLTVKKDEGEGVGGADGAGDHQEPSQETGEATAKESELKQSTEKPEETLKSEQSSTQISLQAESGQAAEEGKDEGEEKQKEPTKSPDSPTSPVASETASPFKKFFTQGWAGWRKKTSFRKPKEDELEASEKKKEQEPEKVDTEGQEKTEDSPEKLAAPEQAHPPEPTDSANAARLSAEYEKVELPSEDQVQGSPEEKPAPLATEVFDDKVEIVAEVHVSMTEKTEEQRAEVEEVVEPLPSEKVAETPAEELVKTKDVCAPGGDHTRPSDLSPDDKVTTAHPEGVVSEVDMLSSQERIKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHHQVAAESPDSTDEQKGESSASSPEEPEEITCLEKGIADAHQDGEAEEGAVSDGEKKREGVTPWASFKKMVTPKKRVRRLSESDKEDELDKVKSATLSSTESAASELQEEAKGNGEEQKPEEPKRKVDTSVSWEALICVGSSKKRARKASSSDDEGEPKAVGGDSQKPDDAGKDKETGPDAALAGSQEHDQGPGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEESVTGSGVEHSASDAEPGKEESWVSIKKFIPGRRKKRSDGKPEQTAVEDAGPAEVNEEDSDVPAVVPLSEYDAVEREKIEAQQAQKSEEQPEQKAAVSVSEELSTDLVRGVTVTVLDGARALTNVEERSPSWISASVTEPLDETEDEAKPLTEDVFEGEVLAEETSVTKPLPEGQDAIDDTVVSEAELTSEAVTAAEATGALCAEEAAEASGAEETADMVSAVSQLTDSPDTTEVATPVQEVEGGVPRLEDQERRTQEVLQAVAEKVREESPLPDTRGPEDTTRTTRGVEAEIPEKVEEAEEDSQRQDLKKETEVPLEERAQETKPETLAQGEVIVQATPESLEKVPQVTDSVESSELRTTCQAETLVGATSESILEQAVAPDSAETLTDSETNGSTPVADFEALHVTQPEQIPGMHEGPEVASGAPPQVTEAEAVPAPEEGPPAPSTFPSEEEDKGHSKMEEILERTDKEVSVEAVPTLSKTDVIEEEGQFADRESKDEALVDGPDVTAAPETTISQEETSDLALQDEATEDPEFQKKEDDTEVQSRTPSPSPVGRELVVQVEKEEIEAKPTQATEEELEQKPTVTVSEELSKELVQTVRVAVIDGEKEVTIWEESCPQLVEEAAVCTEVQVQSSEPLPLTAAVVEEKVLGEAVKSVDASETLEPAGAHLAPEEESSTKDEGLTAQPGAVEVPTGTEARPEPGPGTAPGTASAEPDGGTSAAPEGEKTTPQRQTSEEEDGPAACQEVPAREEDLKAENDLSKLQTESSKLVQNVIQTVVGQLGGAEETAADLQTQAQPLTADGQEASSEARPHVETEGGELQASAPDGTHTVDAKEEPPLTAVEQTPSEVSEASEAVSEAPEKITAIEVESSSVHDQQLEEAALPSEEKKETARTEPVPEDCDGAGSEGRIEESLFESQEDKKGDEVDDPESHHSCSPLEDSEAPGDVSQEAPDTDGPTGKKKEGGQEVEFQEAKVHSEPEDEIQTQTRDETQKQEGEPAKSEPTGS

Target ORF information:

RefSeq Version	XM_533451.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris A-kinase anchoring protein 12 (AKAP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533451.6

ORF Insert Sequence:

ATGGGTGCCG GGAGCTCCAC GGAGCAGCGC AGCCCCGAGC AGCCGGAGGC GGGGAGCACC 
ACGCCGGCCG AGCCCCAGCC CGGCGGCGGC GGCCTTGCGG CGGAGGCGGC TCCCGGCGAG 
CCGGGGGACC CCGCCATCGC CGCCGCGGAC CCCGCCACCA AGCTTCTGCA GAAGAATGGT 
CAGCTGTCTA GTGCCAACGG CTTAGCTGAG GAAGAAGAGT TCAGCCCCCA GGAGGGAGCC 
CTGAACGGCC AGGAGGAAGA AGTCACTGTC ACAGATGTTG GACAGAGAGA GTCTGAAGAT 
GTGAGTGAAA GAGACTCAGA AAAAGACATG GCTGCTGGTT CCGTAGTTGT TCAGGACATC 
ACAAAGGATG GGCAGGAGGA GATGCCTGAG ACCATCGAAC AGATTCCTTC TTCAGAAAGC 
AATTTAGAAG AGCTGATACA ACCTACTGAG GCCCAGGCTA ATGATGTTGG ATTTAAGAAG 
GTGTTTAAGT TTGTCGGCTT TAAATTCACT GTAAAAAAGG ATAAGACCGA GAAGTCTGAC 
ACTGTCCAGC TACTCACTGT CAAAAAAGAT GAAGGTGAAG GAGTGGGAGG AGCAGATGGG 
GCTGGTGACC ACCAGGAGCC CAGCCAGGAG ACTGGAGAAG CAACAGCCAA AGAAAGTGAA 
CTGAAACAAT CCACAGAGAA ACCTGAAGAA ACCCTTAAAA GTGAGCAAAG CAGCACACAA 
ATTTCTCTTC AGGCTGAGTC TGGTCAAGCA GCAGAAGAAG GCAAAGATGA AGGCGAAGAA 
AAGCAGAAAG AACCCACCAA ATCTCCAGAC TCTCCCACTA GTCCAGTGGC CAGTGAAACA 
GCGTCACCCT TCAAAAAATT CTTCACTCAA GGTTGGGCCG GCTGGAGAAA AAAGACCAGT 
TTCAGGAAGC CTAAGGAGGA TGAGCTGGAA GCTTCAGAGA AGAAAAAGGA GCAAGAGCCA 
GAAAAAGTAG ACACAGAAGG ACAGGAAAAG ACCGAAGATT CCCCTGAGAA GTTGGCTGCC 
CCTGAACAGG CGCACCCACC GGAGCCCACC GACAGTGCTA ACGCGGCCAG ATTGTCAGCG 
GAATATGAAA AAGTGGAGCT GCCCTCTGAA GATCAAGTGC AGGGATCTCC TGAAGAGAAA 
CCTGCCCCTT TAGCAACAGA AGTATTTGAT GATAAAGTAG AGATTGTTGC CGAAGTCCAC 
GTTAGCATGA CAGAGAAGAC CGAAGAGCAG AGAGCTGAGG TAGAAGAAGT AGTAGAGCCC 
TTACCATCTG AAAAAGTGGC GGAAACACCT GCTGAGGAGC TGGTGAAGAC CAAAGACGTG 
TGCGCCCCTG GAGGGGACCA CACCCGGCCA AGCGACCTAA GCCCCGATGA CAAAGTGACG 
ACTGCACACC CTGAGGGCGT CGTGAGTGAA GTGGACATGC TGTCCTCGCA AGAGAGAATA 
AAGGTGCAAG GAAGCCCTTT AAAGAAACTT TTTACTAGCA CTGGCTTAAA AAAGCTTTCT 
GGAAAGAAAC AGAAAGGGAA AAGAGGAGGA GGGGATGAAG AGTCGGGGGA ACACCATCAA 
GTTGCAGCAG AGTCTCCAGA CAGTACAGAT GAACAGAAGG GCGAGAGCTC CGCTTCATCC 
CCTGAGGAAC CAGAGGAGAT TACGTGTCTG GAGAAAGGCA TCGCGGACGC CCACCAGGAT 
GGCGAAGCTG AGGAAGGAGC TGTTTCTGAT GGGGAAAAGA AGAGAGAAGG TGTTACTCCC 
TGGGCGTCTT TCAAGAAGAT GGTGACGCCC AAGAAACGCG TTAGGCGGCT TTCAGAGAGT 
GATAAAGAAG ATGAATTGGA TAAAGTCAAG AGTGCCACCT TGTCTTCCAC GGAGAGCGCG 
GCCTCTGAGT TGCAGGAGGA AGCGAAAGGA AATGGAGAAG AGCAGAAACC AGAAGAACCG 
AAGCGCAAAG TTGACACTTC GGTATCTTGG GAAGCTTTAA TTTGTGTGGG ATCATCCAAG 
AAAAGAGCAA GAAAAGCATC ATCTTCTGAT GATGAAGGGG AACCAAAAGC CGTGGGAGGA 
GACAGCCAGA AACCAGATGA TGCAGGAAAA GACAAAGAAA CCGGACCAGA CGCTGCCCTT 
GCTGGTTCCC AAGAACATGA TCAAGGGCCA GGAAGCTCCT CACCTGAGCA AGCTGGAAGC 
CCCACCGAAG GGGAGGGAGT TTCCACCTGG GAGTCCTTTA AACGATTAGT CACTCCAAGG 
AAAAAATCGA AGTCAAAACT GGAAGAGAAA AGTGAAGAGT CTGTAACTGG GTCTGGTGTA 
GAACATTCAG CTTCAGATGC AGAACCTGGG AAAGAAGAGT CTTGGGTTTC GATTAAGAAA 
TTCATTCCCG GACGACGGAA GAAAAGGTCC GATGGGAAAC CAGAACAAAC CGCTGTGGAA 
GACGCAGGAC CAGCAGAGGT CAATGAAGAG GATTCCGATG TCCCGGCCGT GGTTCCCCTG 
TCCGAGTATG ATGCAGTAGA AAGGGAGAAA ATCGAAGCCC AGCAGGCCCA AAAAAGTGAG 
GAGCAGCCTG AGCAGAAGGC CGCTGTTTCT GTGTCAGAGG AGCTCAGTAC GGATCTGGTA 
CGTGGGGTCA CGGTGACTGT CCTTGACGGG GCAAGGGCTC TTACCAATGT GGAAGAAAGG 
TCGCCATCTT GGATATCCGC TTCGGTGACA GAGCCTCTTG ACGAAACGGA AGATGAAGCC 
AAACCCCTAA CCGAGGACGT GTTTGAAGGA GAAGTCCTTG CAGAGGAAAC CTCTGTTACC 
AAACCTCTAC CAGAGGGTCA AGATGCCATT GATGACACAG TCGTGAGTGA GGCGGAATTA 
ACTTCGGAAG CTGTGACAGC CGCGGAAGCT ACCGGGGCCC TTTGTGCTGA AGAAGCAGCA 
GAGGCATCTG GTGCTGAAGA GACCGCCGAC ATGGTGTCCG CTGTTTCTCA GCTAACGGAC 
TCTCCAGACA CCACAGAGGT AGCAACGCCT GTTCAGGAGG TGGAAGGTGG CGTACCACGC 
CTGGAAGACC AAGAGAGGCG GACTCAAGAG GTCCTGCAGG CAGTTGCAGA AAAAGTGAGA 
GAAGAATCAC CACTGCCTGA CACCAGAGGG CCAGAAGACA CCACTCGGAC AACGCGGGGA 
GTAGAAGCCG AGATACCAGA GAAGGTGGAG GAAGCGGAAG AGGATTCACA AAGGCAAGAC 
CTGAAGAAAG AGACGGAGGT ACCGCTGGAA GAACGTGCAC AAGAAACTAA ACCTGAGACT 
TTGGCACAAG GGGAAGTAAT CGTACAGGCC ACCCCAGAAA GCTTGGAGAA AGTTCCTCAA 
GTCACAGACA GCGTGGAGTC CAGTGAGCTT AGAACCACTT GTCAAGCTGA AACCCTGGTT 
GGGGCAACAT CAGAATCGAT TCTGGAACAG GCTGTTGCCC CGGACTCAGC TGAAACCCTC 
ACAGACAGCG AGACCAACGG GAGCACCCCA GTAGCTGATT TTGAAGCTCT CCATGTAACA 
CAACCAGAGC AGATCCCGGG GATGCACGAA GGTCCTGAGG TTGCATCGGG CGCACCGCCC 
CAGGTCACAG AAGCAGAGGC AGTTCCTGCA CCCGAAGAGG GGCCTCCAGC ACCTTCTACT 
TTCCCATCCG AAGAAGAAGA TAAAGGACAT TCAAAAATGG AAGAGATTCT AGAACGCACG 
GACAAAGAGG TATCGGTGGA AGCTGTACCC ACTCTTTCAA AGACCGACGT GATAGAAGAG 
GAAGGCCAGT TCGCCGATAG GGAAAGCAAA GACGAAGCTC TCGTCGACGG CCCTGATGTG 
ACAGCGGCCC CTGAAACAAC CATCAGTCAG GAAGAGACGA GTGACCTTGC CCTTCAAGAT 
GAAGCTACTG AAGACCCCGA ATTTCAGAAG AAGGAGGATG ATACCGAAGT CCAGAGCCGT 
ACACCCTCTC CCAGCCCGGT GGGGAGAGAG CTGGTAGTTC AAGTAGAAAA GGAGGAAATA 
GAAGCAAAGC CAACCCAAGC CACCGAAGAA GAACTTGAGC AAAAACCCAC CGTGACCGTC 
TCTGAGGAGC TTAGCAAGGA ACTGGTGCAG ACAGTTAGGG TGGCCGTCAT CGACGGGGAA 
AAGGAGGTTA CCATTTGGGA AGAAAGTTGT CCCCAGCTAG TTGAAGAGGC AGCGGTATGC 
ACAGAAGTCC AAGTGCAGAG CTCTGAACCG TTACCTCTAA CAGCCGCAGT GGTGGAGGAG 
AAGGTCTTGG GAGAAGCTGT CAAGAGTGTA GATGCCAGTG AGACCCTGGA ACCTGCGGGC 
GCACATTTAG CCCCAGAAGA AGAATCCTCC ACAAAGGATG AAGGCCTGAC GGCCCAGCCA 
GGGGCGGTCG AGGTGCCCAC GGGGACTGAG GCTCGGCCGG AACCTGGACC GGGAACTGCC 
CCGGGAACCG CATCAGCTGA GCCTGACGGA GGCACGAGCG CTGCCCCGGA AGGAGAGAAA 
ACCACCCCCC AGAGACAGAC CTCAGAGGAA GAGGACGGGC CGGCTGCTTG CCAGGAAGTC 
CCGGCAAGAG AGGAGGATCT AAAGGCTGAA AACGACCTCT CGAAACTTCA GACCGAGAGT 
AGTAAACTTG TACAGAATGT GATCCAGACA GTTGTTGGTC AGTTAGGAGG CGCGGAAGAA 
ACAGCCGCGG ACCTCCAGAC CCAGGCTCAG CCGCTGACGG CGGACGGCCA GGAGGCCAGC 
TCGGAGGCCA GGCCGCACGT TGAGACGGAA GGAGGCGAGC TTCAGGCCTC TGCACCAGAT 
GGAACCCACA CTGTTGACGC CAAAGAAGAA CCCCCACTAA CCGCCGTGGA GCAGACCCCT 
TCTGAGGTTT CTGAAGCCTC GGAAGCTGTG AGTGAAGCTC CAGAAAAGAT CACAGCCATC 
GAGGTAGAAA GTTCAAGCGT CCATGACCAG CAGCTTGAAG AGGCAGCTCT CCCATCTGAA 
GAAAAGAAAG AAACAGCCAG AACCGAGCCT GTCCCAGAAG ATTGTGATGG TGCCGGGTCA 
GAAGGAAGGA TAGAGGAGTC GCTCTTTGAA TCCCAAGAAG ATAAAAAAGG TGATGAGGTT 
GATGACCCTG AGAGCCACCA CTCGTGCTCA CCCCTGGAAG ATTCTGAGGC CCCAGGAGAC 
GTAAGCCAAG AGGCCCCGGA CACGGATGGA CCAACAGGCA AAAAGAAGGA AGGTGGCCAG 
GAAGTAGAAT TCCAGGAAGC CAAAGTCCAC AGCGAGCCAG AAGATGAGAT TCAAACCCAA 
ACACGGGACG AGACACAGAA ACAAGAGGGA GAACCGGCCA AATCAGAACC CACAGGATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

AKAP12 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報