CFAP221 cDNA ORF clone, Canis lupus familiaris(dog)

The following CFAP221 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFAP221 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe20984 XM_014121488.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe20984 XM_014121489.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe20984 XM_014121490.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe20984 XM_014121487.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe20984 XM_005631908.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe20985 XM_005631909.2
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe295119 XM_022406059.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe295119 XM_022406060.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe295119 XM_022406058.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe295119 XM_022406057.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe295119 XM_022406055.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe295119 XM_022406056.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe295120 XM_022406061.1
Latest version!
Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OCe20985 XM_005631909.1 Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe20984
    Clone ID Related Accession (Same CDS sequence) XM_005631908.1 , XM_014121487.1 , XM_014121488.1 , XM_014121489.1 , XM_014121490.1
    Accession Version XM_005631908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631965.1
    CDS188..2788
    Translation

    Target ORF information:

    RefSeq Version XM_005631908.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20984
    Clone ID Related Accession (Same CDS sequence) XM_005631908.1 , XM_014121487.1 , XM_014121488.1 , XM_014121489.1 , XM_014121490.1
    Accession Version XM_014121487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976962.1
    CDS204..2804
    Translation

    Target ORF information:

    RefSeq Version XM_014121487.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20984
    Clone ID Related Accession (Same CDS sequence) XM_005631908.1 , XM_014121487.1 , XM_014121488.1 , XM_014121489.1 , XM_014121490.1
    Accession Version XM_014121488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976963.1
    CDS221..2821
    Translation

    Target ORF information:

    RefSeq Version XM_014121488.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20984
    Clone ID Related Accession (Same CDS sequence) XM_005631908.1 , XM_014121487.1 , XM_014121488.1 , XM_014121489.1 , XM_014121490.1
    Accession Version XM_014121489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976964.1
    CDS236..2836
    Translation

    Target ORF information:

    RefSeq Version XM_014121489.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20984
    Clone ID Related Accession (Same CDS sequence) XM_005631908.1 , XM_014121487.1 , XM_014121488.1 , XM_014121489.1 , XM_014121490.1
    Accession Version XM_014121490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976965.1
    CDS68..2668
    Translation

    Target ORF information:

    RefSeq Version XM_014121490.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAAGAGGCCA AAGAACAAGC ACAGCCAATG
    GAGAAGGCAG GAGAAAAGGT GCTGGAGGAG ATGAAGACTC TGCGGGGCAA AGCTCTCAAC
    TCCTACCTGA TTCTAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20985
    Clone ID Related Accession (Same CDS sequence) XM_005631909.1 , XM_005631909.2
    Accession Version XM_005631909.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product cilia- and flagella-associated protein 221 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726093.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGGGAG ATGAGACTCT GCTTATTCCC ATCCATGCCT ATCCTGTCGC CAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCCAAC GTTCTCCTTG GTGAAAGCAA AAAATATGTT
    CTCCCTTTGC AGTGCAACTG CCCTGTAGAC TTCGAGTTTC ATATCACTTT GGTTCAGTCT
    CATGAAGCCT TCACTATTGA CCCAGTATCA GGAATAATCC CAGCTAATGG GAAGATGAAC
    GTGACTGTCA AATTCACACC ATTTCAGTAT GGGATTGCAC AGATAAAAAT GCAGCTGTGG
    ATCTCACAGT TCAACTCTCA GCCATATGAA TGTGTCTTCA CCGGAACGTG CCATCCCAAC
    GTGGCTTTAC CATTAGAAGA ATTTGAAAGA TTAAATGCTC TTTCTAAAAG AGTGGACGTT
    CCTCCAGAAA AGGCGATGAC ACAGATACAT TTTCAGCGAA TGCCACAAAA ACTAAAGCCT
    CCAAAGATTA AGGAAATTGA CTATCAGAAC CTGAGATTTC CAGTAGATTT ATCGAATCCA
    TTTGCTGTCG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TGAAGATTAA AGAATTAAGA
    GAAGTCTTGG ACCAAGGCCA CGAGATTTCA AAAACAAGAC AGATGAAGGA AGCACTCTTT
    GAACAGAAAG TCAGACACGA CACTTATGAA GAGATTGAAA ATCACCTTAA GTGGCAGGTG
    CACCTTGGTA AAGATCATAT GTCTTTTAAA TTTAGAAAAG ACCTCACTGA ACAACGGCAA
    AGAGAAAACG CCAAATATAA GCTAGATAGG GGAGATCCTG TTTTGGATGA GGAATTTCAG
    CGCCTCACGA CGGAAGTCCA CCACAAACGT GTTGTTCACA ACCTAGAAGA AAAAATCAAG
    GAATTCCATC CTAATTTTGA CCCACTCATT AATAATACTT GGGTCAACAG GTTTAGAGCG
    CAGAGGCGGT TTCAACAAGC AGCACGCAAG ATCATGGTTC ATCGAAGATT ACTCAGTATG
    CTGACTGCTA TTCATAATAT AGACAAAGAG ACCATAATCA GAAAGCTGAG CAAAGGAAGA
    CAGTCTATAG TAACAGACAA AAATGCTTCC AGATACCTCC TGGGTCAGAT CAGTCAAGAT
    GATTACTCCA GCAGGTTCTC TGTGTCACCT AAGGAAGTGC TCCCCTTCGC CTTCCCATCC
    TACAGTCCTC CTCAGAGCTC CAATGAGTTG GCTCCTGATG GCCTTGGACC AGTTCCAATT
    AAATCTTCAG AAACTCAAAT CAAGCAGAGT TATTCCTTCC TCAGTCTGCA GGTTCCTCAA
    CAGTACAAAA TTAAAGGGTA TCAGCCATTC TGTGTCCAGA AGTCATCAAC AAGCTACAAA
    CCTCAAAAGC TTGCTCGAGT TCTGAAGCAA GGGGCAGAGG ATGAGGCCAC CATCATTACT
    CTACCCAAGC AGGACTCTAC ACCCCAGCTC CCTGGCAAGA CCTCGGTCTT GGGCATGAGA
    CCACCTGACG CCTTAGCCAT GTCTCCAAAT TATGACCCGC TTTATATATT CAATCCCAGC
    CCAGGATTAT TTGCTGTGAT GCACCCCCTG ACCTACGCAG AAACCCTGAT AGATTACCAT
    CTGTGCCCTC ACCCCAAGTA CAAGTTCATC AAGGAGTCCC ACAGGGGGTC CAGCATTCCT
    CTCACCCAAA AGCAATTTCT CCATCACACG GACATCATTC CGGGGATAAT GCACTGGAAG
    AAGTTCCAGC CCCTGGCTCT CTCATCCCTG CCTGAAACCT CGAAGATAGA CACAACACAG
    AGCTGTGATT CCTTCAATTT AGCCATGCTT CCTAAAAATG TCCCTGCCCT TCTTGACGCC
    CTACCAGAAG AAGACAGATT GGAAACAGCT GAACGTGAGC TCTGCGAACA GAATATAGAA
    GTTATGTTGA CTCCAGAAAT AATTGAAGCA GAATTCCCCA TGTTGGATCA CAAGGACACC
    AAAAAGGAGA AAGAGGCCAA AGAACAAGCA CAGCCAATGG AGAAGGCAGG AGAAAAGGTG
    CTGGAGGAGA TGAAGACTCT GCGGGGCAAA GCTCTCAACT CCTACCTGAT TCTAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631966.1
    CDS188..2707
    Translation

    Target ORF information:

    RefSeq Version XM_005631909.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGGGAG ATGAGACTCT GCTTATTCCC ATCCATGCCT ATCCTGTCGC CAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCCAAC GTTCTCCTTG GTGAAAGCAA AAAATATGTT
    CTCCCTTTGC AGTGCAACTG CCCTGTAGAC TTCGAGTTTC ATATCACTTT GGTTCAGTCT
    CATGAAGCCT TCACTATTGA CCCAGTATCA GGAATAATCC CAGCTAATGG GAAGATGAAC
    GTGACTGTCA AATTCACACC ATTTCAGTAT GGGATTGCAC AGATAAAAAT GCAGCTGTGG
    ATCTCACAGT TCAACTCTCA GCCATATGAA TGTGTCTTCA CCGGAACGTG CCATCCCAAC
    GTGGCTTTAC CATTAGAAGA ATTTGAAAGA TTAAATGCTC TTTCTAAAAG AGTGGACGTT
    CCTCCAGAAA AGGCGATGAC ACAGATACAT TTTCAGCGAA TGCCACAAAA ACTAAAGCCT
    CCAAAGATTA AGGAAATTGA CTATCAGAAC CTGAGATTTC CAGTAGATTT ATCGAATCCA
    TTTGCTGTCG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TGAAGATTAA AGAATTAAGA
    GAAGTCTTGG ACCAAGGCCA CGAGATTTCA AAAACAAGAC AGATGAAGGA AGCACTCTTT
    GAACAGAAAG TCAGACACGA CACTTATGAA GAGATTGAAA ATCACCTTAA GTGGCAGGTG
    CACCTTGGTA AAGATCATAT GTCTTTTAAA TTTAGAAAAG ACCTCACTGA ACAACGGCAA
    AGAGAAAACG CCAAATATAA GCTAGATAGG GGAGATCCTG TTTTGGATGA GGAATTTCAG
    CGCCTCACGA CGGAAGTCCA CCACAAACGT GTTGTTCACA ACCTAGAAGA AAAAATCAAG
    GAATTCCATC CTAATTTTGA CCCACTCATT AATAATACTT GGGTCAACAG GTTTAGAGCG
    CAGAGGCGGT TTCAACAAGC AGCACGCAAG ATCATGGTTC ATCGAAGATT ACTCAGTATG
    CTGACTGCTA TTCATAATAT AGACAAAGAG ACCATAATCA GAAAGCTGAG CAAAGGAAGA
    CAGTCTATAG TAACAGACAA AAATGCTTCC AGATACCTCC TGGGTCAGAT CAGTCAAGAT
    GATTACTCCA GCAGGTTCTC TGTGTCACCT AAGGAAGTGC TCCCCTTCGC CTTCCCATCC
    TACAGTCCTC CTCAGAGCTC CAATGAGTTG GCTCCTGATG GCCTTGGACC AGTTCCAATT
    AAATCTTCAG AAACTCAAAT CAAGCAGAGT TATTCCTTCC TCAGTCTGCA GGTTCCTCAA
    CAGTACAAAA TTAAAGGGTA TCAGCCATTC TGTGTCCAGA AGTCATCAAC AAGCTACAAA
    CCTCAAAAGC TTGCTCGAGT TCTGAAGCAA GGGGCAGAGG ATGAGGCCAC CATCATTACT
    CTACCCAAGC AGGACTCTAC ACCCCAGCTC CCTGGCAAGA CCTCGGTCTT GGGCATGAGA
    CCACCTGACG CCTTAGCCAT GTCTCCAAAT TATGACCCGC TTTATATATT CAATCCCAGC
    CCAGGATTAT TTGCTGTGAT GCACCCCCTG ACCTACGCAG AAACCCTGAT AGATTACCAT
    CTGTGCCCTC ACCCCAAGTA CAAGTTCATC AAGGAGTCCC ACAGGGGGTC CAGCATTCCT
    CTCACCCAAA AGCAATTTCT CCATCACACG GACATCATTC CGGGGATAAT GCACTGGAAG
    AAGTTCCAGC CCCTGGCTCT CTCATCCCTG CCTGAAACCT CGAAGATAGA CACAACACAG
    AGCTGTGATT CCTTCAATTT AGCCATGCTT CCTAAAAATG TCCCTGCCCT TCTTGACGCC
    CTACCAGAAG AAGACAGATT GGAAACAGCT GAACGTGAGC TCTGCGAACA GAATATAGAA
    GTTATGTTGA CTCCAGAAAT AATTGAAGCA GAATTCCCCA TGTTGGATCA CAAGGACACC
    AAAAAGGAGA AAGAGGCCAA AGAACAAGCA CAGCCAATGG AGAAGGCAGG AGAAAAGGTG
    CTGGAGGAGA TGAAGACTCT GCGGGGCAAA GCTCTCAACT CCTACCTGAT TCTAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20985
    Clone ID Related Accession (Same CDS sequence) XM_005631909.1 , XM_005631909.2
    Accession Version XM_005631909.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGGGAG ATGAGACTCT GCTTATTCCC ATCCATGCCT ATCCTGTCGC CAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCCAAC GTTCTCCTTG GTGAAAGCAA AAAATATGTT
    CTCCCTTTGC AGTGCAACTG CCCTGTAGAC TTCGAGTTTC ATATCACTTT GGTTCAGTCT
    CATGAAGCCT TCACTATTGA CCCAGTATCA GGAATAATCC CAGCTAATGG GAAGATGAAC
    GTGACTGTCA AATTCACACC ATTTCAGTAT GGGATTGCAC AGATAAAAAT GCAGCTGTGG
    ATCTCACAGT TCAACTCTCA GCCATATGAA TGTGTCTTCA CCGGAACGTG CCATCCCAAC
    GTGGCTTTAC CATTAGAAGA ATTTGAAAGA TTAAATGCTC TTTCTAAAAG AGTGGACGTT
    CCTCCAGAAA AGGCGATGAC ACAGATACAT TTTCAGCGAA TGCCACAAAA ACTAAAGCCT
    CCAAAGATTA AGGAAATTGA CTATCAGAAC CTGAGATTTC CAGTAGATTT ATCGAATCCA
    TTTGCTGTCG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TGAAGATTAA AGAATTAAGA
    GAAGTCTTGG ACCAAGGCCA CGAGATTTCA AAAACAAGAC AGATGAAGGA AGCACTCTTT
    GAACAGAAAG TCAGACACGA CACTTATGAA GAGATTGAAA ATCACCTTAA GTGGCAGGTG
    CACCTTGGTA AAGATCATAT GTCTTTTAAA TTTAGAAAAG ACCTCACTGA ACAACGGCAA
    AGAGAAAACG CCAAATATAA GCTAGATAGG GGAGATCCTG TTTTGGATGA GGAATTTCAG
    CGCCTCACGA CGGAAGTCCA CCACAAACGT GTTGTTCACA ACCTAGAAGA AAAAATCAAG
    GAATTCCATC CTAATTTTGA CCCACTCATT AATAATACTT GGGTCAACAG GTTTAGAGCG
    CAGAGGCGGT TTCAACAAGC AGCACGCAAG ATCATGGTTC ATCGAAGATT ACTCAGTATG
    CTGACTGCTA TTCATAATAT AGACAAAGAG ACCATAATCA GAAAGCTGAG CAAAGGAAGA
    CAGTCTATAG TAACAGACAA AAATGCTTCC AGATACCTCC TGGGTCAGAT CAGTCAAGAT
    GATTACTCCA GCAGGTTCTC TGTGTCACCT AAGGAAGTGC TCCCCTTCGC CTTCCCATCC
    TACAGTCCTC CTCAGAGCTC CAATGAGTTG GCTCCTGATG GCCTTGGACC AGTTCCAATT
    AAATCTTCAG AAACTCAAAT CAAGCAGAGT TATTCCTTCC TCAGTCTGCA GGTTCCTCAA
    CAGTACAAAA TTAAAGGGTA TCAGCCATTC TGTGTCCAGA AGTCATCAAC AAGCTACAAA
    CCTCAAAAGC TTGCTCGAGT TCTGAAGCAA GGGGCAGAGG ATGAGGCCAC CATCATTACT
    CTACCCAAGC AGGACTCTAC ACCCCAGCTC CCTGGCAAGA CCTCGGTCTT GGGCATGAGA
    CCACCTGACG CCTTAGCCAT GTCTCCAAAT TATGACCCGC TTTATATATT CAATCCCAGC
    CCAGGATTAT TTGCTGTGAT GCACCCCCTG ACCTACGCAG AAACCCTGAT AGATTACCAT
    CTGTGCCCTC ACCCCAAGTA CAAGTTCATC AAGGAGTCCC ACAGGGGGTC CAGCATTCCT
    CTCACCCAAA AGCAATTTCT CCATCACACG GACATCATTC CGGGGATAAT GCACTGGAAG
    AAGTTCCAGC CCCTGGCTCT CTCATCCCTG CCTGAAACCT CGAAGATAGA CACAACACAG
    AGCTGTGATT CCTTCAATTT AGCCATGCTT CCTAAAAATG TCCCTGCCCT TCTTGACGCC
    CTACCAGAAG AAGACAGATT GGAAACAGCT GAACGTGAGC TCTGCGAACA GAATATAGAA
    GTTATGTTGA CTCCAGAAAT AATTGAAGCA GAATTCCCCA TGTTGGATCA CAAGGACACC
    AAAAAGGAGA AAGAGGCCAA AGAACAAGCA CAGCCAATGG AGAAGGCAGG AGAAAAGGTG
    CTGGAGGAGA TGAAGACTCT GCGGGGCAAA GCTCTCAACT CCTACCTGAT TCTAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631966.1
    CDS187..2706
    Translation

    Target ORF information:

    RefSeq Version XM_005631909.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGGGAG ATGAGACTCT GCTTATTCCC ATCCATGCCT ATCCTGTCGC CAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCCAAC GTTCTCCTTG GTGAAAGCAA AAAATATGTT
    CTCCCTTTGC AGTGCAACTG CCCTGTAGAC TTCGAGTTTC ATATCACTTT GGTTCAGTCT
    CATGAAGCCT TCACTATTGA CCCAGTATCA GGAATAATCC CAGCTAATGG GAAGATGAAC
    GTGACTGTCA AATTCACACC ATTTCAGTAT GGGATTGCAC AGATAAAAAT GCAGCTGTGG
    ATCTCACAGT TCAACTCTCA GCCATATGAA TGTGTCTTCA CCGGAACGTG CCATCCCAAC
    GTGGCTTTAC CATTAGAAGA ATTTGAAAGA TTAAATGCTC TTTCTAAAAG AGTGGACGTT
    CCTCCAGAAA AGGCGATGAC ACAGATACAT TTTCAGCGAA TGCCACAAAA ACTAAAGCCT
    CCAAAGATTA AGGAAATTGA CTATCAGAAC CTGAGATTTC CAGTAGATTT ATCGAATCCA
    TTTGCTGTCG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TGAAGATTAA AGAATTAAGA
    GAAGTCTTGG ACCAAGGCCA CGAGATTTCA AAAACAAGAC AGATGAAGGA AGCACTCTTT
    GAACAGAAAG TCAGACACGA CACTTATGAA GAGATTGAAA ATCACCTTAA GTGGCAGGTG
    CACCTTGGTA AAGATCATAT GTCTTTTAAA TTTAGAAAAG ACCTCACTGA ACAACGGCAA
    AGAGAAAACG CCAAATATAA GCTAGATAGG GGAGATCCTG TTTTGGATGA GGAATTTCAG
    CGCCTCACGA CGGAAGTCCA CCACAAACGT GTTGTTCACA ACCTAGAAGA AAAAATCAAG
    GAATTCCATC CTAATTTTGA CCCACTCATT AATAATACTT GGGTCAACAG GTTTAGAGCG
    CAGAGGCGGT TTCAACAAGC AGCACGCAAG ATCATGGTTC ATCGAAGATT ACTCAGTATG
    CTGACTGCTA TTCATAATAT AGACAAAGAG ACCATAATCA GAAAGCTGAG CAAAGGAAGA
    CAGTCTATAG TAACAGACAA AAATGCTTCC AGATACCTCC TGGGTCAGAT CAGTCAAGAT
    GATTACTCCA GCAGGTTCTC TGTGTCACCT AAGGAAGTGC TCCCCTTCGC CTTCCCATCC
    TACAGTCCTC CTCAGAGCTC CAATGAGTTG GCTCCTGATG GCCTTGGACC AGTTCCAATT
    AAATCTTCAG AAACTCAAAT CAAGCAGAGT TATTCCTTCC TCAGTCTGCA GGTTCCTCAA
    CAGTACAAAA TTAAAGGGTA TCAGCCATTC TGTGTCCAGA AGTCATCAAC AAGCTACAAA
    CCTCAAAAGC TTGCTCGAGT TCTGAAGCAA GGGGCAGAGG ATGAGGCCAC CATCATTACT
    CTACCCAAGC AGGACTCTAC ACCCCAGCTC CCTGGCAAGA CCTCGGTCTT GGGCATGAGA
    CCACCTGACG CCTTAGCCAT GTCTCCAAAT TATGACCCGC TTTATATATT CAATCCCAGC
    CCAGGATTAT TTGCTGTGAT GCACCCCCTG ACCTACGCAG AAACCCTGAT AGATTACCAT
    CTGTGCCCTC ACCCCAAGTA CAAGTTCATC AAGGAGTCCC ACAGGGGGTC CAGCATTCCT
    CTCACCCAAA AGCAATTTCT CCATCACACG GACATCATTC CGGGGATAAT GCACTGGAAG
    AAGTTCCAGC CCCTGGCTCT CTCATCCCTG CCTGAAACCT CGAAGATAGA CACAACACAG
    AGCTGTGATT CCTTCAATTT AGCCATGCTT CCTAAAAATG TCCCTGCCCT TCTTGACGCC
    CTACCAGAAG AAGACAGATT GGAAACAGCT GAACGTGAGC TCTGCGAACA GAATATAGAA
    GTTATGTTGA CTCCAGAAAT AATTGAAGCA GAATTCCCCA TGTTGGATCA CAAGGACACC
    AAAAAGGAGA AAGAGGCCAA AGAACAAGCA CAGCCAATGG AGAAGGCAGG AGAAAAGGTG
    CTGGAGGAGA TGAAGACTCT GCGGGGCAAA GCTCTCAACT CCTACCTGAT TCTAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295119
    Clone ID Related Accession (Same CDS sequence) XM_022406057.1 , XM_022406055.1 , XM_022406059.1 , XM_022406056.1 , XM_022406060.1 , XM_022406058.1
    Accession Version XM_022406057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261765.1
    CDS191..3640
    Translation

    Target ORF information:

    RefSeq Version XM_022406057.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295119
    Clone ID Related Accession (Same CDS sequence) XM_022406057.1 , XM_022406055.1 , XM_022406059.1 , XM_022406056.1 , XM_022406060.1 , XM_022406058.1
    Accession Version XM_022406055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261763.1
    CDS189..3638
    Translation

    Target ORF information:

    RefSeq Version XM_022406055.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295119
    Clone ID Related Accession (Same CDS sequence) XM_022406057.1 , XM_022406055.1 , XM_022406059.1 , XM_022406056.1 , XM_022406060.1 , XM_022406058.1
    Accession Version XM_022406059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261767.1
    CDS214..3663
    Translation

    Target ORF information:

    RefSeq Version XM_022406059.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295119
    Clone ID Related Accession (Same CDS sequence) XM_022406057.1 , XM_022406055.1 , XM_022406059.1 , XM_022406056.1 , XM_022406060.1 , XM_022406058.1
    Accession Version XM_022406056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261764.1
    CDS194..3643
    Translation

    Target ORF information:

    RefSeq Version XM_022406056.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295119
    Clone ID Related Accession (Same CDS sequence) XM_022406057.1 , XM_022406055.1 , XM_022406059.1 , XM_022406056.1 , XM_022406060.1 , XM_022406058.1
    Accession Version XM_022406060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261768.1
    CDS202..3651
    Translation

    Target ORF information:

    RefSeq Version XM_022406060.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295119
    Clone ID Related Accession (Same CDS sequence) XM_022406057.1 , XM_022406055.1 , XM_022406059.1 , XM_022406056.1 , XM_022406060.1 , XM_022406058.1
    Accession Version XM_022406058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261766.1
    CDS253..3702
    Translation

    Target ORF information:

    RefSeq Version XM_022406058.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGAAGG AGTATTTGTA CCAAGAAGAT GAATCTCAGG GACAAGTATG GTACAGAGTG
    CCAGGGCACA TCTCCTGCTA CTCAATGGGA GATGAGACTC TGCTTATTCC CATCCATGCC
    TATCCTGTCG CCAACACACT GGACTTTCCC TCATTTATAA ATCTATCCAA CGTTCTCCTT
    GGTGAAAGCA AAAAATATGT TCTCCCTTTG CAGTGCAACT GCCCTGTAGA CTTCGAGTTT
    CATATCACTT TGGTTCAGTC TCATGAAGCC TTCACTATTG ACCCAGTATC AGGAATAATC
    CCAGCTAATG GGAAGATGAA CGTGACTGTC AAATTCACAC CATTTCAGTA TGGGATTGCA
    CAGATAAAAA TGCAGCTGTG GATCTCACAG TTCAACTCTC AGCCATATGA ATGTGTCTTC
    ACCGGAACGT GCCATCCCAA CGTGGCTTTA CCATTAGAAG AATTTGAAAG ATTAAATGCT
    CTTTCTAAAA GAGTGGACGT TCCTCCAGAA AAGGCGATGA CACAGATACA TTTTCAGCGA
    ATGCCACAAA AACTAAAGCC TCCAAAGATT AAGGAAATTG ACTATCAGAA CCTGAGATTT
    CCAGTAGATT TATCGAATCC ATTTGCTGTC GCAACTGTTT TAAACCAAGA ACCAGGAAAA
    TTGAAGATTA AAGAATTAAG AGAAGTCTTG GACCAAGGCC ACGAGATTTC AAAAACAAGA
    CAGATGAAGG AAGCACTCTT TGAACAGAAA GTCAGACACG ACACTTATGA AGAGATTGAA
    AATCACCTTA AGTGGCAGGT GCACCTTGGT AAAGATCATA TGTCTTTTAA ATTTAGAAAA
    GACCTCACTG AACAACGGCA AAGAGAAAAC GCCAAATATA AGCTAGATAG GGGAGATCCT
    GTTTTGGATG AGGAATTTCA GCGCCTCACG ACGGAAGTCC ACCACAAACG TGTTGTTCAC
    AACCTAGAAG AAAAAATCAA GGAATTCCAT CCTAATTTTG ACCCACTCAT TAATAATACT
    TGGGTCAACA GGTTTAGAGC GCAGAGGCGG TTTCAACAAG CAGCACGCAA GATCATGGTT
    CATCGAAGAT TACTCAGTAT GCTGACTGCT ATTCATAATA TAGACAAAGA GACCATAATC
    AGAAAGCTGA GCAAAGGAAG ACAGTCTATA GTAACAGACA AAAATGCTTC CAGATACCTC
    CTGGGTCAGA TCAGTCAAGA TGATTACTCC AGCAGGTTCT CTGTGTCACC TAAGGAAGTG
    CTCCCCTTCG CCTTCCCATC CTACAGTCCT CCTCAGAGCT CCAATGAGTT GGCTCCTGAT
    GGCCTTGGAC CAGTTCCAAT TAAATCTTCA GAAACTCAAA TCAAGCAGAG TTATTCCTTC
    CTCAGTCTGC AGGTTCCTCA ACAGTACAAA ATTAAAGGGT ATCAGCCATT CTGTGTCCAG
    AAGTCATCAA CAAGCTACAA ACCTCAAAAG CTTGCTCGAG TTCTGAAGCA AGGGGCAGAG
    GATGAGGCCA CCATCATTAC TCTACCCAAG CAGGACTCTA CACCCCAGCT CCCTGGCAAG
    ACCTCGGTCT TGGGCATGAG ACCACCTGAC GCCTTAGCCA TGTCTCCAAA TTATGACCCG
    CTTTATATAT TCAATCCCAG CCCAGGATTA TTTGCTGTGA TGCACCCCCT GACCTACGCA
    GAAACCCTGA TAGATTACCA TCTGTGCCCT CACCCCAAGT ACAAGTTCAT CAAGGAGTCC
    CACAGGGGGT CCAGCATTCC TCTCACCCAA AAGCAATTTC TCCATCACAC GGACATCATT
    CCGGGGATAA TGCACTGGAA GAAGTTCCAG CCCCTGGCTC TCTCATCCCT GCCTGAAACC
    TCGAAGATAG ACACAACACA GAGCTGTGAT TCCTTCAATT TAGCCATGCT TCCTAAAAAT
    GTCCCTGCCC TTCTTGACGC CCTACCAGAA GAAGACAGAT TGGAAACAGC TGAACGTGAG
    CTCTGCGAAC AGAATATAGA AGTTATGTTG ACTCCAGAAA TAATTGAAGC AGAATTCCCC
    ATGTTGGATC ACAAGGACAC CAAAAAGGAG AAGTTCACAT CGGTGGCCAC ACTCATGGCA
    GGTCCCCTGT GGCGGGCCGC AGCATTCGTG CAGAGACACA GAGCAGGCCT GTTGGTGGGT
    TCCTGTGCCG GCCTGTTCGG GGCCCAGATC TCATACCACC TCTTCCCAGA TCCTGTGATC
    CGGTGGCTGT ACCAGTACTG GCCTCAGGGC CAGCCAGCTC CGCTCTCCCC AGAGCTGCAG
    AGCCTCTTCC AAGAGGTGCT CCAGGACGTG GGTATCCCCT CGGGCCATAG CTATGAGGCT
    TTCACCTCAT TCACCTTCCA GCCTGTGAGT GCTGGCTTCC CGCGACTCCC TGCTGGGGCG
    ATAGCGGGCA TCCCAGCCAC CTTCCTGGGT GGCACATTGA TCGACAGTGA TCACCCAGTA
    GTTGTACACG GGCAAAGAGT CAACTGGCAG AGCCCAGCAG GCACCCGGCT GAGAGATGCC
    CTGACCCTGT CCCGCGACGC CCAGAAGTTT GCCTTGGCCA AGGAAGTGGT ATACCTGGAG
    AGCAGTACAG CCGCCCTACA GGCCCTGTTG GCCCCAGCTT GCCTGGCAGG CACCTGGGCG
    CTGGGCGTGG GTGCCAAGCA TGCACTGGGG CTCTATGGAG GCCCCATGAA TTTACGGGCT
    GCCTTCAACC TGGCAGCAGC AGTGGCGGGC TTTGTGGCCT ATGCCTTCTC CACGGACTCT
    CTCACTCATG CGCTGGAAGC CTGGCTCGAC CGCCGCACAG CTGCACTGTC TGCAGCCTAT
    GCCCAGGGTG GAGTGGAGTT CTATGAGAAG ATTTTGTCGG GCAACATAGC CCTGCGCAGT
    CTCTTGGGCA AGCGTGGAGA GCAGCTGTAT ACGCCCAGTG GGAACATCGT TCCCAGACAC
    TGGTTTCGCA TCAAACATTT ACCCTACACA ACCCGCCGGG ATTCTGTGCT GCAGATGTGG
    AGGGCAACAC TCAACCCAGG CCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295120
    Clone ID Related Accession (Same CDS sequence) XM_022406061.1
    Accession Version XM_022406061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product cilia- and flagella-associated protein 221 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGGGAG ATGAGACTCT GCTTATTCCC ATCCATGCCT ATCCTGTCGC CAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCCAAC GTTCTCCTTG GTGAAAGCAA AAAATATGTT
    CTCCCTTTGC AGTGCAACTG CCCTGTAGAC TTCGAGTTTC ATATCACTTT GGTTCAGTCT
    CATGAAGCCT TCACTATTGA CCCAGTATCA GGAATAATCC CAGCTAATGG GAAGATGAAC
    GTGACTGTCA AATTCACACC ATTTCAGTAT GGGATTGCAC AGATAAAAAT GCAGCTGTGG
    ATCTCACAGT TCAACTCTCA GCCATATGAA TGTGTCTTCA CCGGAACGTG CCATCCCAAC
    GTGGCTTTAC CATTAGAAGA ATTTGAAAGA TTAAATGCTC TTTCTAAAAG AGTGGACGTT
    CCTCCAGAAA AGGCGATGAC ACAGATACAT TTTCAGCGAA TGCCACAAAA ACTAAAGCCT
    CCAAAGATTA AGGAAATTGA CTATCAGAAC CTGAGATTTC CAGTAGATTT ATCGAATCCA
    TTTGCTGTCG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TGAAGATTAA AGAATTAAGA
    GAAGTCTTGG ACCAAGGCCA CGAGATTTCA AAAACAAGAC AGATGAAGGA AGCACTCTTT
    GAACAGAAAG TCAGACACGA CACTTATGAA GAGATTGAAA ATCACCTTAA GTGGCAGGTG
    CACCTTGGTA AAGATCATAT GTCTTTTAAA TTTAGAAAAG ACCTCACTGA ACAACGGCAA
    AGAGAAAACG CCAAATATAA GCTAGATAGG GGAGATCCTG TTTTGGATGA GGAATTTCAG
    CGCCTCACGA CGGAAGTCCA CCACAAACGT GTTGTTCACA ACCTAGAAGA AAAAATCAAG
    GAATTCCATC CTAATTTTGA CCCACTCATT AATAATACTT GGGTCAACAG GTTTAGAGCG
    CAGAGGCGGT TTCAACAAGC AGCACGCAAG ATCATGGTTC ATCGAAGATT ACTCAGTATG
    CTGACTGCTA TTCATAATAT AGACAAAGAG ACCATAATCA GAAAGCTGAG CAAAGGAAGA
    CAGTCTATAG TAACAGACAA AAATGCTTCC AGATACCTCC TGGGTCAGAT CAGTCAAGAT
    GATTACTCCA GCAGGTTCTC TGTGTCACCT AAGGAAGTGC TCCCCTTCGC CTTCCCATCC
    TACAGTCCTC CTCAGAGCTC CAATGAGTTG GCTCCTGATG GCCTTGGACC AGTTCCAATT
    AAATCTTCAG AAACTCAAAT CAAGCAGAGT TATTCCTTCC TCAGTCTGCA GGTTCCTCAA
    CAGTACAAAA TTAAAGGGTA TCAGCCATTC TGTGTCCAGA AGTCATCAAC AAGCTACAAA
    CCTCAAAAGC TTGCTCGAGT TCTGAAGCAA GGGGCAGAGG ATGAGGCCAC CATCATTACT
    CTACCCAAGC AGGACTCTAC ACCCCAGCTC CCTGGCAAGA CCTCGGTCTT GGGCATGAGA
    CCACCTGACG CCTTAGCCAT GTCTCCAAAT TATGACCCGC TTTATATATT CAATCCCAGC
    CCAGGATTAT TTGCTGTGAT GCACCCCCTG ACCTACGCAG AAACCCTGAT AGATTACCAT
    CTGTGCCCTC ACCCCAAGTA CAAGTTCATC AAGGAGTCCC ACAGGGGGTC CAGCATTCCT
    CTCACCCAAA AGCAATTTCT CCATCACACG GACATCATTC CGGGGATAAT GCACTGGAAG
    AAGTTCCAGC CCCTGGCTCT CTCATCCCTG CCTGAAACCT CGAAGATAGA CACAACACAG
    AGCTGTGATT CCTTCAATTT AGCCATGCTT CCTAAAAATG TCCCTGCCCT TCTTGACGCC
    CTACCAGAAG AAGACAGATT GGAAACAGCT GAACGTGAGC TCTGCGAACA GAATATAGAA
    GTTATGTTGA CTCCAGAAAT AATTGAAGCA GAATTCCCCA TGTTGGATCA CAAGGACACC
    AAAAAGGAGA AGTTCACATC GGTGGCCACA CTCATGGCAG GTCCCCTGTG GCGGGCCGCA
    GCATTCGTGC AGAGACACAG AGCAGGCCTG TTGGTGGGTT CCTGTGCCGG CCTGTTCGGG
    GCCCAGATCT CATACCACCT CTTCCCAGAT CCTGTGATCC GGTGGCTGTA CCAGTACTGG
    CCTCAGGGCC AGCCAGCTCC GCTCTCCCCA GAGCTGCAGA GCCTCTTCCA AGAGGTGCTC
    CAGGACGTGG GTATCCCCTC GGGCCATAGC TATGAGGCTT TCACCTCATT CACCTTCCAG
    CCTGTGAGTG CTGGCTTCCC GCGACTCCCT GCTGGGGCGA TAGCGGGCAT CCCAGCCACC
    TTCCTGGGTG GCACATTGAT CGACAGTGAT CACCCAGTAG TTGTACACGG GCAAAGAGTC
    AACTGGCAGA GCCCAGCAGG CACCCGGCTG AGAGATGCCC TGACCCTGTC CCGCGACGCC
    CAGAAGTTTG CCTTGGCCAA GGAAGTGGTA TACCTGGAGA GCAGTACAGC CGCCCTACAG
    GCCCTGTTGG CCCCAGCTTG CCTGGCAGGC ACCTGGGCGC TGGGCGTGGG TGCCAAGCAT
    GCACTGGGGC TCTATGGAGG CCCCATGAAT TTACGGGCTG CCTTCAACCT GGCAGCAGCA
    GTGGCGGGCT TTGTGGCCTA TGCCTTCTCC ACGGACTCTC TCACTCATGC GCTGGAAGCC
    TGGCTCGACC GCCGCACAGC TGCACTGTCT GCAGCCTATG CCCAGGGTGG AGTGGAGTTC
    TATGAGAAGA TTTTGTCGGG CAACATAGCC CTGCGCAGTC TCTTGGGCAA GCGTGGAGAG
    CAGCTGTATA CGCCCAGTGG GAACATCGTT CCCAGACACT GGTTTCGCAT CAAACATTTA
    CCCTACACAA CCCGCCGGGA TTCTGTGCTG CAGATGTGGA GGGCAACACT CAACCCAGGC
    CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261769.1
    CDS188..3556
    Translation

    Target ORF information:

    RefSeq Version XM_022406061.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cilia and flagella associated protein 221 (CFAP221), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGTGG TGAAAACCCC CAGCAGAGAA CTAAACAGTG CGGAAGAACC TTTTACTAAC 
    ATATCCCCCC TCCTTCTGAA GAGCCTAGTG GAGGAGCCCA AGAAAAGAAC AGAAGTACCT
    AATCACCTTC TAGAATCAAA GGTTTATGCA AAACTTCTGA AAAATAAGGT CATACAGGCA
    AAACCTGGCA TATTGCATTT TGGAGGCTAT CAAGTAGACA AACAACACCA ACAGATTCTG
    AATCTGGTCA ATATTTCCAA CGGAGACATA CATGTGCATA TCTTACCCCC TCAAACCAAA
    TACTTTCAGA TCAAGTATGT GAAAAAGGAA CACTGCCTTG TCCCCGGCTT GTCCCTCACA
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGACTGTAT TCGGGTTCAC
    TGTCAGGGAG ATGAGACTCT GCTTATTCCC ATCCATGCCT ATCCTGTCGC CAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCCAAC GTTCTCCTTG GTGAAAGCAA AAAATATGTT
    CTCCCTTTGC AGTGCAACTG CCCTGTAGAC TTCGAGTTTC ATATCACTTT GGTTCAGTCT
    CATGAAGCCT TCACTATTGA CCCAGTATCA GGAATAATCC CAGCTAATGG GAAGATGAAC
    GTGACTGTCA AATTCACACC ATTTCAGTAT GGGATTGCAC AGATAAAAAT GCAGCTGTGG
    ATCTCACAGT TCAACTCTCA GCCATATGAA TGTGTCTTCA CCGGAACGTG CCATCCCAAC
    GTGGCTTTAC CATTAGAAGA ATTTGAAAGA TTAAATGCTC TTTCTAAAAG AGTGGACGTT
    CCTCCAGAAA AGGCGATGAC ACAGATACAT TTTCAGCGAA TGCCACAAAA ACTAAAGCCT
    CCAAAGATTA AGGAAATTGA CTATCAGAAC CTGAGATTTC CAGTAGATTT ATCGAATCCA
    TTTGCTGTCG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TGAAGATTAA AGAATTAAGA
    GAAGTCTTGG ACCAAGGCCA CGAGATTTCA AAAACAAGAC AGATGAAGGA AGCACTCTTT
    GAACAGAAAG TCAGACACGA CACTTATGAA GAGATTGAAA ATCACCTTAA GTGGCAGGTG
    CACCTTGGTA AAGATCATAT GTCTTTTAAA TTTAGAAAAG ACCTCACTGA ACAACGGCAA
    AGAGAAAACG CCAAATATAA GCTAGATAGG GGAGATCCTG TTTTGGATGA GGAATTTCAG
    CGCCTCACGA CGGAAGTCCA CCACAAACGT GTTGTTCACA ACCTAGAAGA AAAAATCAAG
    GAATTCCATC CTAATTTTGA CCCACTCATT AATAATACTT GGGTCAACAG GTTTAGAGCG
    CAGAGGCGGT TTCAACAAGC AGCACGCAAG ATCATGGTTC ATCGAAGATT ACTCAGTATG
    CTGACTGCTA TTCATAATAT AGACAAAGAG ACCATAATCA GAAAGCTGAG CAAAGGAAGA
    CAGTCTATAG TAACAGACAA AAATGCTTCC AGATACCTCC TGGGTCAGAT CAGTCAAGAT
    GATTACTCCA GCAGGTTCTC TGTGTCACCT AAGGAAGTGC TCCCCTTCGC CTTCCCATCC
    TACAGTCCTC CTCAGAGCTC CAATGAGTTG GCTCCTGATG GCCTTGGACC AGTTCCAATT
    AAATCTTCAG AAACTCAAAT CAAGCAGAGT TATTCCTTCC TCAGTCTGCA GGTTCCTCAA
    CAGTACAAAA TTAAAGGGTA TCAGCCATTC TGTGTCCAGA AGTCATCAAC AAGCTACAAA
    CCTCAAAAGC TTGCTCGAGT TCTGAAGCAA GGGGCAGAGG ATGAGGCCAC CATCATTACT
    CTACCCAAGC AGGACTCTAC ACCCCAGCTC CCTGGCAAGA CCTCGGTCTT GGGCATGAGA
    CCACCTGACG CCTTAGCCAT GTCTCCAAAT TATGACCCGC TTTATATATT CAATCCCAGC
    CCAGGATTAT TTGCTGTGAT GCACCCCCTG ACCTACGCAG AAACCCTGAT AGATTACCAT
    CTGTGCCCTC ACCCCAAGTA CAAGTTCATC AAGGAGTCCC ACAGGGGGTC CAGCATTCCT
    CTCACCCAAA AGCAATTTCT CCATCACACG GACATCATTC CGGGGATAAT GCACTGGAAG
    AAGTTCCAGC CCCTGGCTCT CTCATCCCTG CCTGAAACCT CGAAGATAGA CACAACACAG
    AGCTGTGATT CCTTCAATTT AGCCATGCTT CCTAAAAATG TCCCTGCCCT TCTTGACGCC
    CTACCAGAAG AAGACAGATT GGAAACAGCT GAACGTGAGC TCTGCGAACA GAATATAGAA
    GTTATGTTGA CTCCAGAAAT AATTGAAGCA GAATTCCCCA TGTTGGATCA CAAGGACACC
    AAAAAGGAGA AGTTCACATC GGTGGCCACA CTCATGGCAG GTCCCCTGTG GCGGGCCGCA
    GCATTCGTGC AGAGACACAG AGCAGGCCTG TTGGTGGGTT CCTGTGCCGG CCTGTTCGGG
    GCCCAGATCT CATACCACCT CTTCCCAGAT CCTGTGATCC GGTGGCTGTA CCAGTACTGG
    CCTCAGGGCC AGCCAGCTCC GCTCTCCCCA GAGCTGCAGA GCCTCTTCCA AGAGGTGCTC
    CAGGACGTGG GTATCCCCTC GGGCCATAGC TATGAGGCTT TCACCTCATT CACCTTCCAG
    CCTGTGAGTG CTGGCTTCCC GCGACTCCCT GCTGGGGCGA TAGCGGGCAT CCCAGCCACC
    TTCCTGGGTG GCACATTGAT CGACAGTGAT CACCCAGTAG TTGTACACGG GCAAAGAGTC
    AACTGGCAGA GCCCAGCAGG CACCCGGCTG AGAGATGCCC TGACCCTGTC CCGCGACGCC
    CAGAAGTTTG CCTTGGCCAA GGAAGTGGTA TACCTGGAGA GCAGTACAGC CGCCCTACAG
    GCCCTGTTGG CCCCAGCTTG CCTGGCAGGC ACCTGGGCGC TGGGCGTGGG TGCCAAGCAT
    GCACTGGGGC TCTATGGAGG CCCCATGAAT TTACGGGCTG CCTTCAACCT GGCAGCAGCA
    GTGGCGGGCT TTGTGGCCTA TGCCTTCTCC ACGGACTCTC TCACTCATGC GCTGGAAGCC
    TGGCTCGACC GCCGCACAGC TGCACTGTCT GCAGCCTATG CCCAGGGTGG AGTGGAGTTC
    TATGAGAAGA TTTTGTCGGG CAACATAGCC CTGCGCAGTC TCTTGGGCAA GCGTGGAGAG
    CAGCTGTATA CGCCCAGTGG GAACATCGTT CCCAGACACT GGTTTCGCAT CAAACATTTA
    CCCTACACAA CCCGCCGGGA TTCTGTGCTG CAGATGTGGA GGGCAACACT CAACCCAGGC
    CACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.