ITSN2 cDNA ORF clone, Canis lupus familiaris(Dog)

The following ITSN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe294254 XM_022404502.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40698 XM_014120288.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40698 XM_014120289.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40698 XM_014120290.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40698 XM_014120287.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40699 XM_014120291.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40700 XM_014120292.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40701 XM_022404501.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40701 XM_014120293.2
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40702 XM_014120294.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40703 XM_014120295.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40704 XM_022404503.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40704 XM_014120296.2
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40704 XM_022404504.1
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40705 XM_014120297.2
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe40706 XM_014120298.2
Latest version!
Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OCe40701 XM_014120293.1 Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40705 XM_014120297.1 Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe40704 XM_014120296.1 Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40706 XM_014120298.1 Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe294254
    Clone ID Related Accession (Same CDS sequence) XM_022404502.1
    Accession Version XM_022404502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGGA ACTGAGAGAA
    AGCTACAATA CACAGCAGTT AGCCCTTGAA CAGCTTCACA AGATCAAACG TGACAAGTTG
    AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAAA AGAAACTTGA AGATGAGGCT
    ATAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGGG AAAGTCTTAG AAAGGAAGAA
    GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAGCACAAG AGAAAGTTCA AGAAGAGGAG
    CGGAAAGTGG AGGAGAAACA ATGTGAGCCA GCTAGTGCTT TGGTGAATTA TAAAGCACTG
    TATCGCTTTG AAGCCAGGAG CCATGATGAG ATGAGTTTTA ATTCCGGGGA TATAATACAG
    GTTGATGAAA AAACCATAGG AGAACCTGGT TGGCTTTATG GTAGTTTTCA AGGAAATTTT
    GGCTGGTTTC CTTGCAACTA TGTAGAAAAA ATGCTGTCAG GTGAAAAATC TGTGTCTCCT
    AAGAAGGCCT TACTTCCCCC TACAGTATCT GTATCTGCTA CCTCAACTTC TTCTGAATCA
    CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TACAAACCTA
    ACTGTTAATA CATCATGGCA GAAAAAATCA GCTTTTACTC GTACTGTGTC CCCTGGATCT
    ATATCACCGA TTCATGGACA GGGACAAGTT GTAGAAAACC TAAAGGCACA GGCCCTTTGT
    TCTTGGACTG CAAAGAAAGA TAACCACTTG AACTTTTCAA AACATGATAT TATTACTGTC
    TTGGAGCAAC AAGAAAATTG GTGGTTTGGG GAGGTACATG GAGGAAGAGG ATGGTTTCCA
    AAATCTTATG TCAAAATCAT CCCTGGGAGT GAAGTCAAGC GGGAAGAACC AGAAACCTTG
    TATGCAGCTG TAAACAAGAA ACCTACCTCT GCAGCCTATA CAGTTGGAGA AGAGTATATT
    GCACTTTATT CATATTCAAG TGTAGAACCT GGAGATTTGA CTTTCACTGA AGGTGAAGAA
    ATACTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAACTGGA
    ATTTTTCCAT CAAATTATGT CAAACCAAAA GATCAAGAGA ATTTTGGGAG TGCTAGCAAA
    TCTGGAACGT CAAACAAGAA ACCTGAGATT GCTCAAGTAA CTTCAGCATA TGTAGCTTCT
    GGTTCTGAAC AACTAAGCCT TGCACCAGGG CAGTTAATAT TAATCCTAAA GAAAAATTCA
    AGTGGATGGT GGCAAGGGGA ATTACAGGCC AGAGGAAAAA AGCGACAAAA AGGATGGTTT
    CCTGCCAGCC ATGTTAAACT TTTGGGTCCA AGTAGCGAAA GAACTACACC TGCCTTTCAT
    CCTGTGTGTC AGGTGATTGC TATGTATGAC TATGCGGCAA ATAATGAGGA TGAGCTGAAT
    TTCTCCAAGG GACAACTTAT CAATGTTTTG AACAAAGATG ATCCTGACTG GTGGCAAGGA
    GAAATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTATG TTAAGATGAC AACAGACTCT
    GATCCAAGTC AACAGTGGTG CGCGGATCTC CAGAGCCTGG ACACCATGCA GCCAGTGGAG
    AGGAAGAGGC AGGGCTACAT TCACGAGCTG ATTCAGACTG AAGAGCGGTA CGTGGATGAT
    CTTCAGCTCG TCGTGGAGGT TTTTCAGAAA CGGATGGCAG AGTCAGGGTT TCTCAGTGAA
    GGAGAGATGG CCTTGATATT TGTAAACTGG AAAGAGCTCA TCATGTCTAA CACAAAGCTA
    CTGAAGGCCT TGCGGGTACG GAAGAAGACG GGAGGAGAGA AGATGCCTGT GCAGATGATT
    GGGGATATCC TGGCAGCTGA GCTGTCCCAC ATGCAGGCTT ATATCCGGTT CTGTAGCTGT
    CAGCTCAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGATGCAGA TTTCAAGGAA
    TTTTTAAAGA AGCTGGCATC TGACCCTCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG
    CTGAAGCCCA TGCAGAGGAT CACCCGCTAC CCTCTCCTCA TCAGAAGTAT TCTGGAGAAC
    ACCCTGGAGA ATCATGTAGA CCATTCCTCC CTAAAGCTGG CTCTTGAGCA AGCAGAGGAG
    CTTTGTTCCC AAGTTAATGA AGGAGTTCGG GAAAAAGAAA ATTCAGACCG ACTAGAGTGG
    ATCCAGGCGC ACGTCCAGTG TGAAGGTCTT GCAGAGCAAC TTATTTTCAA CTCACTCACC
    AACTGCCTGG GGCCCCGGAA GCTTCTGCAC AGTGGGAAAT TATACAAAAC CAAGAGCAAC
    AAGGAGCTAC ACGGATTCCT CTTCAACGAC TTTCTGCTTC TGACCTACAT GGTCAAGCAG
    TTCGCTGTTT CCTCAGGCTC TGAGAAGCTT TTCAGCTCTA AGTCCAATGC CCAGTTCAAA
    ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC GGACCCTCCC
    AGCGATGAGC CGGTCTTCCA CATTTCTCAC ATTGACAGAG TCTACACCCT TCGAACAGAC
    AACATTAACG AGAGGACTGC CTGGGTCCAG AAGATCAAGG CGGCATCTGA GCAGTACATC
    GATACGGAAA AGAAGAAACG GGAAAAAGCT TATCAAGCTC GCTCTCAAAA GACCTCAGGC
    ATTGGGCGTC TGATGGTGCA GGTCATCGAA GCTACAGAAT TAAAAGCCTG TAAACCAAAT
    GGAAAGAGCA ATCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTATAC CACCAGAACC
    CTCCCGGACA CACTCAATCC CAAATGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC
    TACCAAGATG TGCTGTGTCT CACCATGTTT GACAGAGATC AGTTTTCACC AGATGATTTC
    CTGGGTCGTA CTGAAGTTCC AGTGGCAAAA ATTCGCACAG AACAGGAAAG CAAAGGCCCC
    ACAACCCGCC GACTGCTGCT GCATGAGGTC CCCACTGGGG AGGTCTGGGT CCGCTTTGAC
    CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260210.1
    CDS192..5264
    Translation

    Target ORF information:

    RefSeq Version XM_022404502.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGGA ACTGAGAGAA
    AGCTACAATA CACAGCAGTT AGCCCTTGAA CAGCTTCACA AGATCAAACG TGACAAGTTG
    AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAAA AGAAACTTGA AGATGAGGCT
    ATAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGGG AAAGTCTTAG AAAGGAAGAA
    GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAGCACAAG AGAAAGTTCA AGAAGAGGAG
    CGGAAAGTGG AGGAGAAACA ATGTGAGCCA GCTAGTGCTT TGGTGAATTA TAAAGCACTG
    TATCGCTTTG AAGCCAGGAG CCATGATGAG ATGAGTTTTA ATTCCGGGGA TATAATACAG
    GTTGATGAAA AAACCATAGG AGAACCTGGT TGGCTTTATG GTAGTTTTCA AGGAAATTTT
    GGCTGGTTTC CTTGCAACTA TGTAGAAAAA ATGCTGTCAG GTGAAAAATC TGTGTCTCCT
    AAGAAGGCCT TACTTCCCCC TACAGTATCT GTATCTGCTA CCTCAACTTC TTCTGAATCA
    CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TACAAACCTA
    ACTGTTAATA CATCATGGCA GAAAAAATCA GCTTTTACTC GTACTGTGTC CCCTGGATCT
    ATATCACCGA TTCATGGACA GGGACAAGTT GTAGAAAACC TAAAGGCACA GGCCCTTTGT
    TCTTGGACTG CAAAGAAAGA TAACCACTTG AACTTTTCAA AACATGATAT TATTACTGTC
    TTGGAGCAAC AAGAAAATTG GTGGTTTGGG GAGGTACATG GAGGAAGAGG ATGGTTTCCA
    AAATCTTATG TCAAAATCAT CCCTGGGAGT GAAGTCAAGC GGGAAGAACC AGAAACCTTG
    TATGCAGCTG TAAACAAGAA ACCTACCTCT GCAGCCTATA CAGTTGGAGA AGAGTATATT
    GCACTTTATT CATATTCAAG TGTAGAACCT GGAGATTTGA CTTTCACTGA AGGTGAAGAA
    ATACTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAACTGGA
    ATTTTTCCAT CAAATTATGT CAAACCAAAA GATCAAGAGA ATTTTGGGAG TGCTAGCAAA
    TCTGGAACGT CAAACAAGAA ACCTGAGATT GCTCAAGTAA CTTCAGCATA TGTAGCTTCT
    GGTTCTGAAC AACTAAGCCT TGCACCAGGG CAGTTAATAT TAATCCTAAA GAAAAATTCA
    AGTGGATGGT GGCAAGGGGA ATTACAGGCC AGAGGAAAAA AGCGACAAAA AGGATGGTTT
    CCTGCCAGCC ATGTTAAACT TTTGGGTCCA AGTAGCGAAA GAACTACACC TGCCTTTCAT
    CCTGTGTGTC AGGTGATTGC TATGTATGAC TATGCGGCAA ATAATGAGGA TGAGCTGAAT
    TTCTCCAAGG GACAACTTAT CAATGTTTTG AACAAAGATG ATCCTGACTG GTGGCAAGGA
    GAAATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTATG TTAAGATGAC AACAGACTCT
    GATCCAAGTC AACAGTGGTG CGCGGATCTC CAGAGCCTGG ACACCATGCA GCCAGTGGAG
    AGGAAGAGGC AGGGCTACAT TCACGAGCTG ATTCAGACTG AAGAGCGGTA CGTGGATGAT
    CTTCAGCTCG TCGTGGAGGT TTTTCAGAAA CGGATGGCAG AGTCAGGGTT TCTCAGTGAA
    GGAGAGATGG CCTTGATATT TGTAAACTGG AAAGAGCTCA TCATGTCTAA CACAAAGCTA
    CTGAAGGCCT TGCGGGTACG GAAGAAGACG GGAGGAGAGA AGATGCCTGT GCAGATGATT
    GGGGATATCC TGGCAGCTGA GCTGTCCCAC ATGCAGGCTT ATATCCGGTT CTGTAGCTGT
    CAGCTCAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGATGCAGA TTTCAAGGAA
    TTTTTAAAGA AGCTGGCATC TGACCCTCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG
    CTGAAGCCCA TGCAGAGGAT CACCCGCTAC CCTCTCCTCA TCAGAAGTAT TCTGGAGAAC
    ACCCTGGAGA ATCATGTAGA CCATTCCTCC CTAAAGCTGG CTCTTGAGCA AGCAGAGGAG
    CTTTGTTCCC AAGTTAATGA AGGAGTTCGG GAAAAAGAAA ATTCAGACCG ACTAGAGTGG
    ATCCAGGCGC ACGTCCAGTG TGAAGGTCTT GCAGAGCAAC TTATTTTCAA CTCACTCACC
    AACTGCCTGG GGCCCCGGAA GCTTCTGCAC AGTGGGAAAT TATACAAAAC CAAGAGCAAC
    AAGGAGCTAC ACGGATTCCT CTTCAACGAC TTTCTGCTTC TGACCTACAT GGTCAAGCAG
    TTCGCTGTTT CCTCAGGCTC TGAGAAGCTT TTCAGCTCTA AGTCCAATGC CCAGTTCAAA
    ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC GGACCCTCCC
    AGCGATGAGC CGGTCTTCCA CATTTCTCAC ATTGACAGAG TCTACACCCT TCGAACAGAC
    AACATTAACG AGAGGACTGC CTGGGTCCAG AAGATCAAGG CGGCATCTGA GCAGTACATC
    GATACGGAAA AGAAGAAACG GGAAAAAGCT TATCAAGCTC GCTCTCAAAA GACCTCAGGC
    ATTGGGCGTC TGATGGTGCA GGTCATCGAA GCTACAGAAT TAAAAGCCTG TAAACCAAAT
    GGAAAGAGCA ATCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTATAC CACCAGAACC
    CTCCCGGACA CACTCAATCC CAAATGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC
    TACCAAGATG TGCTGTGTCT CACCATGTTT GACAGAGATC AGTTTTCACC AGATGATTTC
    CTGGGTCGTA CTGAAGTTCC AGTGGCAAAA ATTCGCACAG AACAGGAAAG CAAAGGCCCC
    ACAACCCGCC GACTGCTGCT GCATGAGGTC CCCACTGGGG AGGTCTGGGT CCGCTTTGAC
    CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40698
    Clone ID Related Accession (Same CDS sequence) XM_014120287.1 , XM_014120288.1 , XM_014120289.1 , XM_014120290.1
    Accession Version XM_014120287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975762.1
    CDS180..5453
    Translation

    Target ORF information:

    RefSeq Version XM_014120287.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40698
    Clone ID Related Accession (Same CDS sequence) XM_014120287.1 , XM_014120288.1 , XM_014120289.1 , XM_014120290.1
    Accession Version XM_014120288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975763.1
    CDS270..5543
    Translation

    Target ORF information:

    RefSeq Version XM_014120288.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40698
    Clone ID Related Accession (Same CDS sequence) XM_014120287.1 , XM_014120288.1 , XM_014120289.1 , XM_014120290.1
    Accession Version XM_014120289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975764.1
    CDS59..5332
    Translation

    Target ORF information:

    RefSeq Version XM_014120289.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40698
    Clone ID Related Accession (Same CDS sequence) XM_014120287.1 , XM_014120288.1 , XM_014120289.1 , XM_014120290.1
    Accession Version XM_014120290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975765.1
    CDS130..5403
    Translation

    Target ORF information:

    RefSeq Version XM_014120290.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    AGTTGGTGGT GCCTGGGGAG TTGGGAGCAG CTGCCAGGGG ACCCCAGGGA GGGGCGAGGT
    CCCACCCTCA GGACACAAGC TGCTGCTGCC CCTGCAGTGA GGGCACTAAA CAGGGACAGC
    CTGAAAGACC AGACTTGCTG GGGGACCAGA CAGCAGAGCC CACTTGGCTG CTGCCGCCCG
    TGGGAACTCC CTGAGCCCCC TAGCCACTAC ACGGCGGCGA GGAGCATCCA GTGCCAGCCC
    CAGGACCTCC CTTCTGTCTG CGGCAAAGGA GGAGGGTCCT CTGCAAAACC ACGCCCACTT
    CTGCTTGGTC CATTTGCTTC ACCCAACTTC ACGTCTGTGG CCTGTTTGCC TTCAGCTCTG
    TGCCTTCTCT CTGACCGCAG CCTCTGCCCC AGCCCTCCTC CACATCCACC CATCCTCCCT
    CTCTGGACAA CAGCACCTGG ATCTCAAGGC TCTCCTCCCC ATCACCAGCC CTTCATCCCA
    CTTGCTGTCC CTATCCTGTG CAGATGGCTC CCTCACTTGA CACCAGTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40699
    Clone ID Related Accession (Same CDS sequence) XM_014120291.1
    Accession Version XM_014120291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGGA ACTGAGAGAA
    AGCTACAATA CACAGCAGTT AGCCCTTGAA CAGCTTCACA AGATCAAACG TGACAAGTTG
    AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAAA AGAAACTTGA AGATGAGGCT
    ATAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGGG AAAGTCTTAG AAAGGAAGAA
    GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAGCACAAG AGAAAGTTCA AGAAGAGGAG
    CGGAAAGTGG AGGAGAAACA ATGTGAGCCA GCTAGTGCTT TGGTGAATTA TAAAGCACTG
    TATCGCTTTG AAGCCAGGAG CCATGATGAG ATGAGTTTTA ATTCCGGGGA TATAATACAG
    GTTGATGAAA AAACCATAGG AGAACCTGGT TGGCTTTATG GTAGTTTTCA AGGAAATTTT
    GGCTGGTTTC CTTGCAACTA TGTAGAAAAA ATGCTGTCAG GTGAAAAATC TGTGTCTCCT
    AAGAAGGCCT TACTTCCCCC TACAGTATCT GTATCTGCTA CCTCAACTTC TTCTGAATCA
    CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TACAAACCTA
    ACTGTTAATA CATCATGGCA GAAAAAATCA GCTTTTACTC GTACTGTGTC CCCTGGATCT
    ATATCACCGA TTCATGGACA GGGACAAGTT GTAGAAAACC TAAAGGCACA GGCCCTTTGT
    TCTTGGACTG CAAAGAAAGA TAACCACTTG AACTTTTCAA AACATGATAT TATTACTGTC
    TTGGAGCAAC AAGAAAATTG GTGGTTTGGG GAGGTACATG GAGGAAGAGG ATGGTTTCCA
    AAATCTTATG TCAAAATCAT CCCTGGGAGT GAAGTCAAGC GGGAAGAACC AGAAACCTTG
    TATGCAGCTG TAAACAAGAA ACCTACCTCT GCAGCCTATA CAGTTGGAGA AGAGTATATT
    GCACTTTATT CATATTCAAG TGTAGAACCT GGAGATTTGA CTTTCACTGA AGGTGAAGAA
    ATACTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAACTGGA
    ATTTTTCCAT CAAATTATGT CAAACCAAAA GATCAAGAGA ATTTTGGGAG TGCTAGCAAA
    TCTGGAACGT CAAACAAGAA ACCTGAGATT GCTCAAGTAA CTTCAGCATA TGTAGCTTCT
    GGTTCTGAAC AACTAAGCCT TGCACCAGGG CAGTTAATAT TAATCCTAAA GAAAAATTCA
    AGTGGATGGT GGCAAGGGGA ATTACAGGCC AGAGGAAAAA AGCGACAAAA AGGATGGTTT
    CCTGCCAGCC ATGTTAAACT TTTGGGTCCA AGTAGCGAAA GAACTACACC TGCCTTTCAT
    CCTGTGTGTC AGGTGATTGC TATGTATGAC TATGCGGCAA ATAATGAGGA TGAGCTGAAT
    TTCTCCAAGG GACAACTTAT CAATGTTTTG AACAAAGATG ATCCTGACTG GTGGCAAGGA
    GAAATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTATG TTAAGATGAC AACAGACTCT
    GATCCAAGTC AACAGTGGTG CGCGGATCTC CAGAGCCTGG ACACCATGCA GCCAGTGGAG
    AGGAAGAGGC AGGGCTACAT TCACGAGCTG ATTCAGACTG AAGAGCGGTA CGTGGATGAT
    CTTCAGCTCG TCGTGGAGGT TTTTCAGAAA CGGATGGCAG AGTCAGGGTT TCTCAGTGAA
    GGAGAGATGG CCTTGATATT TGTAAACTGG AAAGAGCTCA TCATGTCTAA CACAAAGCTA
    CTGAAGGCCT TGCGGGTACG GAAGAAGACG GGAGGAGAGA AGATGCCTGT GCAGATGATT
    GGGGATATCC TGGCAGCTGA GCTGTCCCAC ATGCAGGCTT ATATCCGGTT CTGTAGCTGT
    CAGCTCAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGATGCAGA TTTCAAGGAA
    TTTTTAAAGA AGCTGGCATC TGACCCTCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG
    CTGAAGCCCA TGCAGAGGAT CACCCGCTAC CCTCTCCTCA TCAGAAGTAT TCTGGAGAAC
    ACCCTGGAGA ATCATGTAGA CCATTCCTCC CTAAAGCTGG CTCTTGAGCA AGCAGAGGAG
    CTTTGTTCCC AAGTTAATGA AGGAGTTCGG GAAAAAGAAA ATTCAGACCG ACTAGAGTGG
    ATCCAGGCGC ACGTCCAGTG TGAAGGTCTT GCAGAGCAAC TTATTTTCAA CTCACTCACC
    AACTGCCTGG GGCCCCGGAA GCTTCTGCAC AGTGGGAAAT TATACAAAAC CAAGAGCAAC
    AAGGAGCTAC ACGGATTCCT CTTCAACGAC TTTCTGCTTC TGACCTACAT GGTCAAGCAG
    TTCGCTGTTT CCTCAGGCTC TGAGAAGCTT TTCAGCTCTA AGTCCAATGC CCAGTTCAAA
    ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC GGACCCTCCC
    AGCGATGAGC CGGTCTTCCA CATTTCTCAC ATTGACAGAG TCTACACCCT TCGAACAGAC
    AACATTAACG AGAGGACTGC CTGGGTCCAG AAGATCAAGG CGGCATCTGA GCAGTACATC
    GATACGGAAA AGAAGAAACG GGAAAAAGCT TATCAAGGTA GTTGGTGGTG CCTGGGGAGT
    TGGGAGCAGC TGCCAGGGGA CCCCAGGGAG GGGCGAGGTC CCACCCTCAG GACACAAGCT
    GCTGCTGCCC CTGCAGTGAG GGCACTAAAC AGGGACAGCC TGAAAGACCA GACTTGCTGG
    GGGACCAGAC AGCAGAGCCC ACTTGGCTGC TGCCGCCCGT GGGAACTCCC TGAGCCCCCT
    AGCCACTACA CGGCGGCGAG GAGCATCCAG TGCCAGCCCC AGGACCTCCC TTCTGTCTGC
    GGCAAAGGAG GAGGGTCCTC TGCAAAACCA CGCCCACTTC TGCTTGGTCC ATTTGCTTCA
    CCCAACTTCA CGTCTGTGGC CTGTTTGCCT TCAGCTCTGT GCCTTCTCTC TGACCGCAGC
    CTCTGCCCCA GCCCTCCTCC ACATCCACCC ATCCTCCCTC TCTGGACAAC AGCACCTGGA
    TCTCAAGGCT CTCCTCCCCA TCACCAGCCC TTCATCCCAC TTGCTGTCCC TATCCTGTGC
    AGATGGCTCC CTCACTTGAC ACCAGTGACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975766.1
    CDS179..5371
    Translation

    Target ORF information:

    RefSeq Version XM_014120291.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGGA ACTGAGAGAA
    AGCTACAATA CACAGCAGTT AGCCCTTGAA CAGCTTCACA AGATCAAACG TGACAAGTTG
    AAGGAAATTG AAAGAAAAAG GTCAGAGCTA ATACAGAAAA AGAAACTTGA AGATGAGGCT
    ATAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAGGG AAAGTCTTAG AAAGGAAGAA
    GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAGCACAAG AGAAAGTTCA AGAAGAGGAG
    CGGAAAGTGG AGGAGAAACA ATGTGAGCCA GCTAGTGCTT TGGTGAATTA TAAAGCACTG
    TATCGCTTTG AAGCCAGGAG CCATGATGAG ATGAGTTTTA ATTCCGGGGA TATAATACAG
    GTTGATGAAA AAACCATAGG AGAACCTGGT TGGCTTTATG GTAGTTTTCA AGGAAATTTT
    GGCTGGTTTC CTTGCAACTA TGTAGAAAAA ATGCTGTCAG GTGAAAAATC TGTGTCTCCT
    AAGAAGGCCT TACTTCCCCC TACAGTATCT GTATCTGCTA CCTCAACTTC TTCTGAATCA
    CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TACAAACCTA
    ACTGTTAATA CATCATGGCA GAAAAAATCA GCTTTTACTC GTACTGTGTC CCCTGGATCT
    ATATCACCGA TTCATGGACA GGGACAAGTT GTAGAAAACC TAAAGGCACA GGCCCTTTGT
    TCTTGGACTG CAAAGAAAGA TAACCACTTG AACTTTTCAA AACATGATAT TATTACTGTC
    TTGGAGCAAC AAGAAAATTG GTGGTTTGGG GAGGTACATG GAGGAAGAGG ATGGTTTCCA
    AAATCTTATG TCAAAATCAT CCCTGGGAGT GAAGTCAAGC GGGAAGAACC AGAAACCTTG
    TATGCAGCTG TAAACAAGAA ACCTACCTCT GCAGCCTATA CAGTTGGAGA AGAGTATATT
    GCACTTTATT CATATTCAAG TGTAGAACCT GGAGATTTGA CTTTCACTGA AGGTGAAGAA
    ATACTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAACTGGA
    ATTTTTCCAT CAAATTATGT CAAACCAAAA GATCAAGAGA ATTTTGGGAG TGCTAGCAAA
    TCTGGAACGT CAAACAAGAA ACCTGAGATT GCTCAAGTAA CTTCAGCATA TGTAGCTTCT
    GGTTCTGAAC AACTAAGCCT TGCACCAGGG CAGTTAATAT TAATCCTAAA GAAAAATTCA
    AGTGGATGGT GGCAAGGGGA ATTACAGGCC AGAGGAAAAA AGCGACAAAA AGGATGGTTT
    CCTGCCAGCC ATGTTAAACT TTTGGGTCCA AGTAGCGAAA GAACTACACC TGCCTTTCAT
    CCTGTGTGTC AGGTGATTGC TATGTATGAC TATGCGGCAA ATAATGAGGA TGAGCTGAAT
    TTCTCCAAGG GACAACTTAT CAATGTTTTG AACAAAGATG ATCCTGACTG GTGGCAAGGA
    GAAATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTATG TTAAGATGAC AACAGACTCT
    GATCCAAGTC AACAGTGGTG CGCGGATCTC CAGAGCCTGG ACACCATGCA GCCAGTGGAG
    AGGAAGAGGC AGGGCTACAT TCACGAGCTG ATTCAGACTG AAGAGCGGTA CGTGGATGAT
    CTTCAGCTCG TCGTGGAGGT TTTTCAGAAA CGGATGGCAG AGTCAGGGTT TCTCAGTGAA
    GGAGAGATGG CCTTGATATT TGTAAACTGG AAAGAGCTCA TCATGTCTAA CACAAAGCTA
    CTGAAGGCCT TGCGGGTACG GAAGAAGACG GGAGGAGAGA AGATGCCTGT GCAGATGATT
    GGGGATATCC TGGCAGCTGA GCTGTCCCAC ATGCAGGCTT ATATCCGGTT CTGTAGCTGT
    CAGCTCAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGATGCAGA TTTCAAGGAA
    TTTTTAAAGA AGCTGGCATC TGACCCTCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG
    CTGAAGCCCA TGCAGAGGAT CACCCGCTAC CCTCTCCTCA TCAGAAGTAT TCTGGAGAAC
    ACCCTGGAGA ATCATGTAGA CCATTCCTCC CTAAAGCTGG CTCTTGAGCA AGCAGAGGAG
    CTTTGTTCCC AAGTTAATGA AGGAGTTCGG GAAAAAGAAA ATTCAGACCG ACTAGAGTGG
    ATCCAGGCGC ACGTCCAGTG TGAAGGTCTT GCAGAGCAAC TTATTTTCAA CTCACTCACC
    AACTGCCTGG GGCCCCGGAA GCTTCTGCAC AGTGGGAAAT TATACAAAAC CAAGAGCAAC
    AAGGAGCTAC ACGGATTCCT CTTCAACGAC TTTCTGCTTC TGACCTACAT GGTCAAGCAG
    TTCGCTGTTT CCTCAGGCTC TGAGAAGCTT TTCAGCTCTA AGTCCAATGC CCAGTTCAAA
    ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC GGACCCTCCC
    AGCGATGAGC CGGTCTTCCA CATTTCTCAC ATTGACAGAG TCTACACCCT TCGAACAGAC
    AACATTAACG AGAGGACTGC CTGGGTCCAG AAGATCAAGG CGGCATCTGA GCAGTACATC
    GATACGGAAA AGAAGAAACG GGAAAAAGCT TATCAAGGTA GTTGGTGGTG CCTGGGGAGT
    TGGGAGCAGC TGCCAGGGGA CCCCAGGGAG GGGCGAGGTC CCACCCTCAG GACACAAGCT
    GCTGCTGCCC CTGCAGTGAG GGCACTAAAC AGGGACAGCC TGAAAGACCA GACTTGCTGG
    GGGACCAGAC AGCAGAGCCC ACTTGGCTGC TGCCGCCCGT GGGAACTCCC TGAGCCCCCT
    AGCCACTACA CGGCGGCGAG GAGCATCCAG TGCCAGCCCC AGGACCTCCC TTCTGTCTGC
    GGCAAAGGAG GAGGGTCCTC TGCAAAACCA CGCCCACTTC TGCTTGGTCC ATTTGCTTCA
    CCCAACTTCA CGTCTGTGGC CTGTTTGCCT TCAGCTCTGT GCCTTCTCTC TGACCGCAGC
    CTCTGCCCCA GCCCTCCTCC ACATCCACCC ATCCTCCCTC TCTGGACAAC AGCACCTGGA
    TCTCAAGGCT CTCCTCCCCA TCACCAGCCC TTCATCCCAC TTGCTGTCCC TATCCTGTGC
    AGATGGCTCC CTCACTTGAC ACCAGTGACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40700
    Clone ID Related Accession (Same CDS sequence) XM_014120292.1
    Accession Version XM_014120292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGGTAG TTGGTGGTGC CTGGGGAGTT GGGAGCAGCT GCCAGGGGAC
    CCCAGGGAGG GGCGAGGTCC CACCCTCAGG ACACAAGCTG CTGCTGCCCC TGCAGTGAGG
    GCACTAAACA GGGACAGCCT GAAAGACCAG ACTTGCTGGG GGACCAGACA GCAGAGCCCA
    CTTGGCTGCT GCCGCCCGTG GGAACTCCCT GAGCCCCCTA GCCACTACAC GGCGGCGAGG
    AGCATCCAGT GCCAGCCCCA GGACCTCCCT TCTGTCTGCG GCAAAGGAGG AGGGTCCTCT
    GCAAAACCAC GCCCACTTCT GCTTGGTCCA TTTGCTTCAC CCAACTTCAC GTCTGTGGCC
    TGTTTGCCTT CAGCTCTGTG CCTTCTCTCT GACCGCAGCC TCTGCCCCAG CCCTCCTCCA
    CATCCACCCA TCCTCCCTCT CTGGACAACA GCACCTGGAT CTCAAGGCTC TCCTCCCCAT
    CACCAGCCCT TCATCCCACT TGCTGTCCCT ATCCTGTGCA GATGGCTCCC TCACTTGACA
    CCAGTGACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975767.1
    CDS270..5441
    Translation

    Target ORF information:

    RefSeq Version XM_014120292.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGGTAG TTGGTGGTGC CTGGGGAGTT GGGAGCAGCT GCCAGGGGAC
    CCCAGGGAGG GGCGAGGTCC CACCCTCAGG ACACAAGCTG CTGCTGCCCC TGCAGTGAGG
    GCACTAAACA GGGACAGCCT GAAAGACCAG ACTTGCTGGG GGACCAGACA GCAGAGCCCA
    CTTGGCTGCT GCCGCCCGTG GGAACTCCCT GAGCCCCCTA GCCACTACAC GGCGGCGAGG
    AGCATCCAGT GCCAGCCCCA GGACCTCCCT TCTGTCTGCG GCAAAGGAGG AGGGTCCTCT
    GCAAAACCAC GCCCACTTCT GCTTGGTCCA TTTGCTTCAC CCAACTTCAC GTCTGTGGCC
    TGTTTGCCTT CAGCTCTGTG CCTTCTCTCT GACCGCAGCC TCTGCCCCAG CCCTCCTCCA
    CATCCACCCA TCCTCCCTCT CTGGACAACA GCACCTGGAT CTCAAGGCTC TCCTCCCCAT
    CACCAGCCCT TCATCCCACT TGCTGTCCCT ATCCTGTGCA GATGGCTCCC TCACTTGACA
    CCAGTGACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40701
    Clone ID Related Accession (Same CDS sequence) XM_022404501.1 , XM_014120293.1 , XM_014120293.2
    Accession Version XM_022404501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGCT
    CGCTCTCAAA AGACCTCAGG CATTGGGCGT CTGATGGTGC AGGTCATCGA AGCTACAGAA
    TTAAAAGCCT GTAAACCAAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC
    CAGAGCTATA CCACCAGAAC CCTCCCGGAC ACACTCAATC CCAAATGGAA TTTTAACTGC
    CAGTTCTTTA TTAAGGATCT CTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAT
    CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAGTTC CAGTGGCAAA AATTCGCACA
    GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTGCTGC TGCATGAGGT CCCCACTGGG
    GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260209.1
    CDS123..5276
    Translation

    Target ORF information:

    RefSeq Version XM_022404501.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGCT
    CGCTCTCAAA AGACCTCAGG CATTGGGCGT CTGATGGTGC AGGTCATCGA AGCTACAGAA
    TTAAAAGCCT GTAAACCAAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC
    CAGAGCTATA CCACCAGAAC CCTCCCGGAC ACACTCAATC CCAAATGGAA TTTTAACTGC
    CAGTTCTTTA TTAAGGATCT CTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAT
    CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAGTTC CAGTGGCAAA AATTCGCACA
    GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTGCTGC TGCATGAGGT CCCCACTGGG
    GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40701
    Clone ID Related Accession (Same CDS sequence) XM_022404501.1 , XM_014120293.1 , XM_014120293.2
    Accession Version XM_014120293.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGCT
    CGCTCTCAAA AGACCTCAGG CATTGGGCGT CTGATGGTGC AGGTCATCGA AGCTACAGAA
    TTAAAAGCCT GTAAACCAAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC
    CAGAGCTATA CCACCAGAAC CCTCCCGGAC ACACTCAATC CCAAATGGAA TTTTAACTGC
    CAGTTCTTTA TTAAGGATCT CTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAT
    CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAGTTC CAGTGGCAAA AATTCGCACA
    GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTGCTGC TGCATGAGGT CCCCACTGGG
    GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975768.1
    CDS177..5330
    Translation

    Target ORF information:

    RefSeq Version XM_014120293.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGCT
    CGCTCTCAAA AGACCTCAGG CATTGGGCGT CTGATGGTGC AGGTCATCGA AGCTACAGAA
    TTAAAAGCCT GTAAACCAAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC
    CAGAGCTATA CCACCAGAAC CCTCCCGGAC ACACTCAATC CCAAATGGAA TTTTAACTGC
    CAGTTCTTTA TTAAGGATCT CTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAT
    CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAGTTC CAGTGGCAAA AATTCGCACA
    GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTGCTGC TGCATGAGGT CCCCACTGGG
    GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40701
    Clone ID Related Accession (Same CDS sequence) XM_022404501.1 , XM_014120293.1 , XM_014120293.2
    Accession Version XM_014120293.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGCT
    CGCTCTCAAA AGACCTCAGG CATTGGGCGT CTGATGGTGC AGGTCATCGA AGCTACAGAA
    TTAAAAGCCT GTAAACCAAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC
    CAGAGCTATA CCACCAGAAC CCTCCCGGAC ACACTCAATC CCAAATGGAA TTTTAACTGC
    CAGTTCTTTA TTAAGGATCT CTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAT
    CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAGTTC CAGTGGCAAA AATTCGCACA
    GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTGCTGC TGCATGAGGT CCCCACTGGG
    GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975768.1
    CDS193..5346
    Translation

    Target ORF information:

    RefSeq Version XM_014120293.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120293.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGCT
    CGCTCTCAAA AGACCTCAGG CATTGGGCGT CTGATGGTGC AGGTCATCGA AGCTACAGAA
    TTAAAAGCCT GTAAACCAAA TGGAAAGAGC AATCCATACT GTGAAATCAG CATGGGCTCC
    CAGAGCTATA CCACCAGAAC CCTCCCGGAC ACACTCAATC CCAAATGGAA TTTTAACTGC
    CAGTTCTTTA TTAAGGATCT CTACCAAGAT GTGCTGTGTC TCACCATGTT TGACAGAGAT
    CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAGTTC CAGTGGCAAA AATTCGCACA
    GAACAGGAAA GCAAAGGCCC CACAACCCGC CGACTGCTGC TGCATGAGGT CCCCACTGGG
    GAGGTCTGGG TCCGCTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40702
    Clone ID Related Accession (Same CDS sequence) XM_014120294.1
    Accession Version XM_014120294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTGGGCTA TTACCTCTGA AGAACGGACT AAGCATGACA AGCAATTTGA TAACCTCAAA 
    CCCTCAGGAG GTTATATAAC AGGGGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAGCAGG AGTTCTCCAT AGCAATGAAA CTCATCAAAC TAAAGCTGCA AGGCCAACAG
    TTGCCCGTAG TCCTCCCTCC TATTATGAAA CAACCTCCTA TGTTCTCTCC TTTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCACTCATT GCCTCCAGTT
    GCACCTATGA CAACCCCCTT ATCCTCTGCG ACTTCAGGGA CCACCCTTCC TCCCGTAATG
    ATGCCGGCTC CCCTAGTGCC TTCTGTTAGC ACATCATCAC TACCAAATGG AACTCCTGGC
    CTCATTCAGC CTTTATCCAT TCCTTACTCT TCTTCAACAT TGCCTCATAC ATCATCTTAC
    AGTCTCATGA TGGGAGGACT CGGCGGTGCT AGTTTACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC GTCCTCAACT GCTTCACTAT CTGGGAACTC GCCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGGTACCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATTTGGA CTCTGGCTGA TATTGATGGG
    GACGGACAGC TGAAAGCTGA AGAATTCATC CTTGCAATGC ACCTCACTGA CATGGCCAAA
    GCTGGACAGC CGTTGCCACT GACGTTACCT CCTGAGCTTG TCCCTCCGTC TTTCAGAGGA
    GGAAAGCAGA TTGATTCTAT TAATGGAACT CTGCCTTCAT ATCAGAAAAT GCAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACTTTC GAGGACAAGC GGAAGGCCAA CTACGAGCGA
    GGGAATGTGG AACTGGAGAA GCGGCGCCAA GCCTTGATGG AGCAGCAGCA GAGGGAGGCA
    GAACGTAAAG CCCAGAAAGA AAAGGAAGAG TGGGAGCGAA AACAGAGAGA ATTACAGGAG
    CAAGAATGGA AGAAACAACT CGAATTAGAA AAACGCTTGG AGAAGCAAAG GGAATTGGAG
    AGACAGCGGG AGGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGGATTCGTC GACAAGAGCT TCTCAATCAA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG TCTTCATCTT
    GAGCTGGAAG CGCTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCCGAAAAC AAACTCAAAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATTATGGAAA TCAAGCAACT CCAACAAGAA CTTCAGGATT ATCAAAATAA GCTTATCTAT
    TTGGTACCTG AGAAGCAATT ATTAAATGAA AGAATTAAGA ACATGCAACT TGGTAACACA
    CCTGATTTAG GGATTGGTTT GCTTCATAAA AAATCATTGG AAAAGGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT ATCTGAAATG
    GATTCATTTA ACAACCAACT AAAGTGTGGG AATATGGATG ACTCTGTTCT GCAGTGCCTT
    TTGTCTCTGC TAAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT GAGAGAAAGC
    TACAATACAC AGCAGTTAGC CCTTGAACAG CTTCACAAGA TCAAACGTGA CAAGTTGAAG
    GAAATTGAAA GAAAAAGGTC AGAGCTAATA CAGAAAAAGA AACTTGAAGA TGAGGCTATA
    AGGAAAGCAA AGCAAGGAAA AGAAAACTTA TGGAGGGAAA GTCTTAGAAA GGAAGAAGAA
    GAAAAACAAA AGCGACTCCA GGAAGAAAAA GCACAAGAGA AAGTTCAAGA AGAGGAGCGG
    AAAGTGGAGG AGAAACAATG TGAGCCAGCT AGTGCTTTGG TGAATTATAA AGCACTGTAT
    CGCTTTGAAG CCAGGAGCCA TGATGAGATG AGTTTTAATT CCGGGGATAT AATACAGGTT
    GATGAAAAAA CCATAGGAGA ACCTGGTTGG CTTTATGGTA GTTTTCAAGG AAATTTTGGC
    TGGTTTCCTT GCAACTATGT AGAAAAAATG CTGTCAGGTG AAAAATCTGT GTCTCCTAAG
    AAGGCCTTAC TTCCCCCTAC AGTATCTGTA TCTGCTACCT CAACTTCTTC TGAATCACTT
    TCTTCAAATC AACCAGCATC AGTGACTGAT TATCAAAATG TATCTTTTAC AAACCTAACT
    GTTAATACAT CATGGCAGAA AAAATCAGCT TTTACTCGTA CTGTGTCCCC TGGATCTATA
    TCACCGATTC ATGGACAGGG ACAAGTTGTA GAAAACCTAA AGGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGATAA CCACTTGAAC TTTTCAAAAC ATGATATTAT TACTGTCTTG
    GAGCAACAAG AAAATTGGTG GTTTGGGGAG GTACATGGAG GAAGAGGATG GTTTCCAAAA
    TCTTATGTCA AAATCATCCC TGGGAGTGAA GTCAAGCGGG AAGAACCAGA AACCTTGTAT
    GCAGCTGTAA ACAAGAAACC TACCTCTGCA GCCTATACAG TTGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    CTGGTGACCC AGAAAGATGG AGAGTGGTGG ACAGGAAGTA TTGGAGATAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAAGAGAATT TTGGGAGTGC TAGCAAATCT
    GGAACGTCAA ACAAGAAACC TGAGATTGCT CAAGTAACTT CAGCATATGT AGCTTCTGGT
    TCTGAACAAC TAAGCCTTGC ACCAGGGCAG TTAATATTAA TCCTAAAGAA AAATTCAAGT
    GGATGGTGGC AAGGGGAATT ACAGGCCAGA GGAAAAAAGC GACAAAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGCGAAAGAA CTACACCTGC CTTTCATCCT
    GTGTGTCAGG TGATTGCTAT GTATGACTAT GCGGCAAATA ATGAGGATGA GCTGAATTTC
    TCCAAGGGAC AACTTATCAA TGTTTTGAAC AAAGATGATC CTGACTGGTG GCAAGGAGAA
    ATCAACGGGG TGACTGGTCT CTTTCCTTCA AACTATGTTA AGATGACAAC AGACTCTGAT
    CCAAGTCAAC AGTGGTGCGC GGATCTCCAG AGCCTGGACA CCATGCAGCC AGTGGAGAGG
    AAGAGGCAGG GCTACATTCA CGAGCTGATT CAGACTGAAG AGCGGTACGT GGATGATCTT
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGG ATGGCAGAGT CAGGGTTTCT CAGTGAAGGA
    GAGATGGCCT TGATATTTGT AAACTGGAAA GAGCTCATCA TGTCTAACAC AAAGCTACTG
    AAGGCCTTGC GGGTACGGAA GAAGACGGGA GGAGAGAAGA TGCCTGTGCA GATGATTGGG
    GATATCCTGG CAGCTGAGCT GTCCCACATG CAGGCTTATA TCCGGTTCTG TAGCTGTCAG
    CTCAATGGAG CAGCTCTGTT ACAGCAGAAG ACAGATGAAG ATGCAGATTT CAAGGAATTT
    TTAAAGAAGC TGGCATCTGA CCCTCGGTGT AAAGGAATGC CCCTCTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCT CTCCTCATCA GAAGTATTCT GGAGAACACC
    CTGGAGAATC ATGTAGACCA TTCCTCCCTA AAGCTGGCTC TTGAGCAAGC AGAGGAGCTT
    TGTTCCCAAG TTAATGAAGG AGTTCGGGAA AAAGAAAATT CAGACCGACT AGAGTGGATC
    CAGGCGCACG TCCAGTGTGA AGGTCTTGCA GAGCAACTTA TTTTCAACTC ACTCACCAAC
    TGCCTGGGGC CCCGGAAGCT TCTGCACAGT GGGAAATTAT ACAAAACCAA GAGCAACAAG
    GAGCTACACG GATTCCTCTT CAACGACTTT CTGCTTCTGA CCTACATGGT CAAGCAGTTC
    GCTGTTTCCT CAGGCTCTGA GAAGCTTTTC AGCTCTAAGT CCAATGCCCA GTTCAAAATG
    TATAAAACGC CCATTTTCCT GAATGAAGTC TTGGTGAAAC TGCCCACGGA CCCTCCCAGC
    GATGAGCCGG TCTTCCACAT TTCTCACATT GACAGAGTCT ACACCCTTCG AACAGACAAC
    ATTAACGAGA GGACTGCCTG GGTCCAGAAG ATCAAGGCGG CATCTGAGCA GTACATCGAT
    ACGGAAAAGA AGAAACGGGA AAAAGCTTAT CAAGGTAGTT GGTGGTGCCT GGGGAGTTGG
    GAGCAGCTGC CAGGGGACCC CAGGGAGGGG CGAGGTCCCA CCCTCAGGAC ACAAGCTGCT
    GCTGCCCCTG CAGTGAGGGC ACTAAACAGG GACAGCCTGA AAGACCAGAC TTGCTGGGGG
    ACCAGACAGC AGAGCCCACT TGGCTGCTGC CGCCCGTGGG AACTCCCTGA GCCCCCTAGC
    CACTACACGG CGGCGAGGAG CATCCAGTGC CAGCCCCAGG ACCTCCCTTC TGTCTGCGGC
    AAAGGAGGAG GGTCCTCTGC AAAACCACGC CCACTTCTGC TTGGTCCATT TGCTTCACCC
    AACTTCACGT CTGTGGCCTG TTTGCCTTCA GCTCTGTGCC TTCTCTCTGA CCGCAGCCTC
    TGCCCCAGCC CTCCTCCACA TCCACCCATC CTCCCTCTCT GGACAACAGC ACCTGGATCT
    CAAGGCTCTC CTCCCCATCA CCAGCCCTTC ATCCCACTTG CTGTCCCTAT CCTGTGCAGA
    TGGCTCCCTC ACTTGACACC AGTGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975769.1
    CDS104..5233
    Translation

    Target ORF information:

    RefSeq Version XM_014120294.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTGGGCTA TTACCTCTGA AGAACGGACT AAGCATGACA AGCAATTTGA TAACCTCAAA 
    CCCTCAGGAG GTTATATAAC AGGGGATCAA GCCCGTACCT TTTTCTTACA GTCAGGCCTG
    CCGGCTCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TGAACAAGGA TGGGAAGATG
    GATCAGCAGG AGTTCTCCAT AGCAATGAAA CTCATCAAAC TAAAGCTGCA AGGCCAACAG
    TTGCCCGTAG TCCTCCCTCC TATTATGAAA CAACCTCCTA TGTTCTCTCC TTTAATTTCT
    GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCACTCATT GCCTCCAGTT
    GCACCTATGA CAACCCCCTT ATCCTCTGCG ACTTCAGGGA CCACCCTTCC TCCCGTAATG
    ATGCCGGCTC CCCTAGTGCC TTCTGTTAGC ACATCATCAC TACCAAATGG AACTCCTGGC
    CTCATTCAGC CTTTATCCAT TCCTTACTCT TCTTCAACAT TGCCTCATAC ATCATCTTAC
    AGTCTCATGA TGGGAGGACT CGGCGGTGCT AGTTTACAGA AAGCCCAGTC TCTGATTGAT
    TTAGGATCTA GTAGCTCAAC GTCCTCAACT GCTTCACTAT CTGGGAACTC GCCCAAGACT
    GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AATATCGGCA AAAATTTAAT
    AGTCTTGACA AAAGCATGAG TGGGTACCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT
    CAGTCAAATC TTTCTCAAAC TCAGCTAGCT ACTATTTGGA CTCTGGCTGA TATTGATGGG
    GACGGACAGC TGAAAGCTGA AGAATTCATC CTTGCAATGC ACCTCACTGA CATGGCCAAA
    GCTGGACAGC CGTTGCCACT GACGTTACCT CCTGAGCTTG TCCCTCCGTC TTTCAGAGGA
    GGAAAGCAGA TTGATTCTAT TAATGGAACT CTGCCTTCAT ATCAGAAAAT GCAAGAAGAG
    GAACCTCAGA AAAAATTACC AGTTACTTTC GAGGACAAGC GGAAGGCCAA CTACGAGCGA
    GGGAATGTGG AACTGGAGAA GCGGCGCCAA GCCTTGATGG AGCAGCAGCA GAGGGAGGCA
    GAACGTAAAG CCCAGAAAGA AAAGGAAGAG TGGGAGCGAA AACAGAGAGA ATTACAGGAG
    CAAGAATGGA AGAAACAACT CGAATTAGAA AAACGCTTGG AGAAGCAAAG GGAATTGGAG
    AGACAGCGGG AGGAAGAAAG GAGAAAAGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAA
    CTTGAAAGAC AACGTCGTTT AGAATGGGAG AGGATTCGTC GACAAGAGCT TCTCAATCAA
    AGGAATAGAG AACAGGAAGA AATTGTCAGG TTAAACTCTA AAAAGAAGAG TCTTCATCTT
    GAGCTGGAAG CGCTGAATGG CAAACATCAG CAGATCTCAG GCAGACTTCA AGATGTCCGA
    CTCCGAAAAC AAACTCAAAA GACTGAGCTG GAAGTTCTGG ATAAGCAGTG TGACCTGGAA
    ATTATGGAAA TCAAGCAACT CCAACAAGAA CTTCAGGATT ATCAAAATAA GCTTATCTAT
    TTGGTACCTG AGAAGCAATT ATTAAATGAA AGAATTAAGA ACATGCAACT TGGTAACACA
    CCTGATTTAG GGATTGGTTT GCTTCATAAA AAATCATTGG AAAAGGAAGA ATTATGCCAA
    AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT ATCTGAAATG
    GATTCATTTA ACAACCAACT AAAGTGTGGG AATATGGATG ACTCTGTTCT GCAGTGCCTT
    TTGTCTCTGC TAAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT GAGAGAAAGC
    TACAATACAC AGCAGTTAGC CCTTGAACAG CTTCACAAGA TCAAACGTGA CAAGTTGAAG
    GAAATTGAAA GAAAAAGGTC AGAGCTAATA CAGAAAAAGA AACTTGAAGA TGAGGCTATA
    AGGAAAGCAA AGCAAGGAAA AGAAAACTTA TGGAGGGAAA GTCTTAGAAA GGAAGAAGAA
    GAAAAACAAA AGCGACTCCA GGAAGAAAAA GCACAAGAGA AAGTTCAAGA AGAGGAGCGG
    AAAGTGGAGG AGAAACAATG TGAGCCAGCT AGTGCTTTGG TGAATTATAA AGCACTGTAT
    CGCTTTGAAG CCAGGAGCCA TGATGAGATG AGTTTTAATT CCGGGGATAT AATACAGGTT
    GATGAAAAAA CCATAGGAGA ACCTGGTTGG CTTTATGGTA GTTTTCAAGG AAATTTTGGC
    TGGTTTCCTT GCAACTATGT AGAAAAAATG CTGTCAGGTG AAAAATCTGT GTCTCCTAAG
    AAGGCCTTAC TTCCCCCTAC AGTATCTGTA TCTGCTACCT CAACTTCTTC TGAATCACTT
    TCTTCAAATC AACCAGCATC AGTGACTGAT TATCAAAATG TATCTTTTAC AAACCTAACT
    GTTAATACAT CATGGCAGAA AAAATCAGCT TTTACTCGTA CTGTGTCCCC TGGATCTATA
    TCACCGATTC ATGGACAGGG ACAAGTTGTA GAAAACCTAA AGGCACAGGC CCTTTGTTCT
    TGGACTGCAA AGAAAGATAA CCACTTGAAC TTTTCAAAAC ATGATATTAT TACTGTCTTG
    GAGCAACAAG AAAATTGGTG GTTTGGGGAG GTACATGGAG GAAGAGGATG GTTTCCAAAA
    TCTTATGTCA AAATCATCCC TGGGAGTGAA GTCAAGCGGG AAGAACCAGA AACCTTGTAT
    GCAGCTGTAA ACAAGAAACC TACCTCTGCA GCCTATACAG TTGGAGAAGA GTATATTGCA
    CTTTATTCAT ATTCAAGTGT AGAACCTGGA GATTTGACTT TCACTGAAGG TGAAGAAATA
    CTGGTGACCC AGAAAGATGG AGAGTGGTGG ACAGGAAGTA TTGGAGATAG AACTGGAATT
    TTTCCATCAA ATTATGTCAA ACCAAAAGAT CAAGAGAATT TTGGGAGTGC TAGCAAATCT
    GGAACGTCAA ACAAGAAACC TGAGATTGCT CAAGTAACTT CAGCATATGT AGCTTCTGGT
    TCTGAACAAC TAAGCCTTGC ACCAGGGCAG TTAATATTAA TCCTAAAGAA AAATTCAAGT
    GGATGGTGGC AAGGGGAATT ACAGGCCAGA GGAAAAAAGC GACAAAAAGG ATGGTTTCCT
    GCCAGCCATG TTAAACTTTT GGGTCCAAGT AGCGAAAGAA CTACACCTGC CTTTCATCCT
    GTGTGTCAGG TGATTGCTAT GTATGACTAT GCGGCAAATA ATGAGGATGA GCTGAATTTC
    TCCAAGGGAC AACTTATCAA TGTTTTGAAC AAAGATGATC CTGACTGGTG GCAAGGAGAA
    ATCAACGGGG TGACTGGTCT CTTTCCTTCA AACTATGTTA AGATGACAAC AGACTCTGAT
    CCAAGTCAAC AGTGGTGCGC GGATCTCCAG AGCCTGGACA CCATGCAGCC AGTGGAGAGG
    AAGAGGCAGG GCTACATTCA CGAGCTGATT CAGACTGAAG AGCGGTACGT GGATGATCTT
    CAGCTCGTCG TGGAGGTTTT TCAGAAACGG ATGGCAGAGT CAGGGTTTCT CAGTGAAGGA
    GAGATGGCCT TGATATTTGT AAACTGGAAA GAGCTCATCA TGTCTAACAC AAAGCTACTG
    AAGGCCTTGC GGGTACGGAA GAAGACGGGA GGAGAGAAGA TGCCTGTGCA GATGATTGGG
    GATATCCTGG CAGCTGAGCT GTCCCACATG CAGGCTTATA TCCGGTTCTG TAGCTGTCAG
    CTCAATGGAG CAGCTCTGTT ACAGCAGAAG ACAGATGAAG ATGCAGATTT CAAGGAATTT
    TTAAAGAAGC TGGCATCTGA CCCTCGGTGT AAAGGAATGC CCCTCTCCAG CTTCCTGCTG
    AAGCCCATGC AGAGGATCAC CCGCTACCCT CTCCTCATCA GAAGTATTCT GGAGAACACC
    CTGGAGAATC ATGTAGACCA TTCCTCCCTA AAGCTGGCTC TTGAGCAAGC AGAGGAGCTT
    TGTTCCCAAG TTAATGAAGG AGTTCGGGAA AAAGAAAATT CAGACCGACT AGAGTGGATC
    CAGGCGCACG TCCAGTGTGA AGGTCTTGCA GAGCAACTTA TTTTCAACTC ACTCACCAAC
    TGCCTGGGGC CCCGGAAGCT TCTGCACAGT GGGAAATTAT ACAAAACCAA GAGCAACAAG
    GAGCTACACG GATTCCTCTT CAACGACTTT CTGCTTCTGA CCTACATGGT CAAGCAGTTC
    GCTGTTTCCT CAGGCTCTGA GAAGCTTTTC AGCTCTAAGT CCAATGCCCA GTTCAAAATG
    TATAAAACGC CCATTTTCCT GAATGAAGTC TTGGTGAAAC TGCCCACGGA CCCTCCCAGC
    GATGAGCCGG TCTTCCACAT TTCTCACATT GACAGAGTCT ACACCCTTCG AACAGACAAC
    ATTAACGAGA GGACTGCCTG GGTCCAGAAG ATCAAGGCGG CATCTGAGCA GTACATCGAT
    ACGGAAAAGA AGAAACGGGA AAAAGCTTAT CAAGGTAGTT GGTGGTGCCT GGGGAGTTGG
    GAGCAGCTGC CAGGGGACCC CAGGGAGGGG CGAGGTCCCA CCCTCAGGAC ACAAGCTGCT
    GCTGCCCCTG CAGTGAGGGC ACTAAACAGG GACAGCCTGA AAGACCAGAC TTGCTGGGGG
    ACCAGACAGC AGAGCCCACT TGGCTGCTGC CGCCCGTGGG AACTCCCTGA GCCCCCTAGC
    CACTACACGG CGGCGAGGAG CATCCAGTGC CAGCCCCAGG ACCTCCCTTC TGTCTGCGGC
    AAAGGAGGAG GGTCCTCTGC AAAACCACGC CCACTTCTGC TTGGTCCATT TGCTTCACCC
    AACTTCACGT CTGTGGCCTG TTTGCCTTCA GCTCTGTGCC TTCTCTCTGA CCGCAGCCTC
    TGCCCCAGCC CTCCTCCACA TCCACCCATC CTCCCTCTCT GGACAACAGC ACCTGGATCT
    CAAGGCTCTC CTCCCCATCA CCAGCCCTTC ATCCCACTTG CTGTCCCTAT CCTGTGCAGA
    TGGCTCCCTC ACTTGACACC AGTGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40703
    Clone ID Related Accession (Same CDS sequence) XM_014120295.1
    Accession Version XM_014120295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA GCTTCACAAG
    ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAAAAG
    AAACTTGAAG ATGAGGCTAT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGGGAA
    AGTCTTAGAA AGGAAGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AGCACAAGAG
    AAAGTTCAAG AAGAGGAGCG GAAAGTGGAG GAGAAACAAT GTGAGCCAGC TAGTGCTTTG
    GTGAATTATA AAGCACTGTA TCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT
    TCCGGGGATA TAATACAGGT TGATGAAAAA ACCATAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCTGTCAGGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCTGT ATCTGCTACC
    TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT
    GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT
    ACTGTGTCCC CTGGATCTAT ATCACCGATT CATGGACAGG GACAAGTTGT AGAAAACCTA
    AAGGCACAGG CCCTTTGTTC TTGGACTGCA AAGAAAGATA ACCACTTGAA CTTTTCAAAA
    CATGATATTA TTACTGTCTT GGAGCAACAA GAAAATTGGT GGTTTGGGGA GGTACATGGA
    GGAAGAGGAT GGTTTCCAAA ATCTTATGTC AAAATCATCC CTGGGAGTGA AGTCAAGCGG
    GAAGAACCAG AAACCTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTATACA
    GTTGGAGAAG AGTATATTGC ACTTTATTCA TATTCAAGTG TAGAACCTGG AGATTTGACT
    TTCACTGAAG GTGAAGAAAT ACTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT
    ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAAT
    TTTGGGAGTG CTAGCAAATC TGGAACGTCA AACAAGAAAC CTGAGATTGC TCAAGTAACT
    TCAGCATATG TAGCTTCTGG TTCTGAACAA CTAAGCCTTG CACCAGGGCA GTTAATATTA
    ATCCTAAAGA AAAATTCAAG TGGATGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG
    CGACAAAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGCGAAAGA
    ACTACACCTG CCTTTCATCC TGTGTGTCAG GTGATTGCTA TGTATGACTA TGCGGCAAAT
    AATGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT
    CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT
    AAGATGACAA CAGACTCTGA TCCAAGTCAA CAGTGGTGCG CGGATCTCCA GAGCCTGGAC
    ACCATGCAGC CAGTGGAGAG GAAGAGGCAG GGCTACATTC ACGAGCTGAT TCAGACTGAA
    GAGCGGTACG TGGATGATCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGCAGAG
    TCAGGGTTTC TCAGTGAAGG AGAGATGGCC TTGATATTTG TAAACTGGAA AGAGCTCATC
    ATGTCTAACA CAAAGCTACT GAAGGCCTTG CGGGTACGGA AGAAGACGGG AGGAGAGAAG
    ATGCCTGTGC AGATGATTGG GGATATCCTG GCAGCTGAGC TGTCCCACAT GCAGGCTTAT
    ATCCGGTTCT GTAGCTGTCA GCTCAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA
    GATGCAGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGGTG TAAAGGAATG
    CCCCTCTCCA GCTTCCTGCT GAAGCCCATG CAGAGGATCA CCCGCTACCC TCTCCTCATC
    AGAAGTATTC TGGAGAACAC CCTGGAGAAT CATGTAGACC ATTCCTCCCT AAAGCTGGCT
    CTTGAGCAAG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT
    TCAGACCGAC TAGAGTGGAT CCAGGCGCAC GTCCAGTGTG AAGGTCTTGC AGAGCAACTT
    ATTTTCAACT CACTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAATTA
    TACAAAACCA AGAGCAACAA GGAGCTACAC GGATTCCTCT TCAACGACTT TCTGCTTCTG
    ACCTACATGG TCAAGCAGTT CGCTGTTTCC TCAGGCTCTG AGAAGCTTTT CAGCTCTAAG
    TCCAATGCCC AGTTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA
    CTGCCCACGG ACCCTCCCAG CGATGAGCCG GTCTTCCACA TTTCTCACAT TGACAGAGTC
    TACACCCTTC GAACAGACAA CATTAACGAG AGGACTGCCT GGGTCCAGAA GATCAAGGCG
    GCATCTGAGC AGTACATCGA TACGGAAAAG AAGAAACGGG AAAAAGCTTA TCAAGGTAGT
    TGGTGGTGCC TGGGGAGTTG GGAGCAGCTG CCAGGGGACC CCAGGGAGGG GCGAGGTCCC
    ACCCTCAGGA CACAAGCTGC TGCTGCCCCT GCAGTGAGGG CACTAAACAG GGACAGCCTG
    AAAGACCAGA CTTGCTGGGG GACCAGACAG CAGAGCCCAC TTGGCTGCTG CCGCCCGTGG
    GAACTCCCTG AGCCCCCTAG CCACTACACG GCGGCGAGGA GCATCCAGTG CCAGCCCCAG
    GACCTCCCTT CTGTCTGCGG CAAAGGAGGA GGGTCCTCTG CAAAACCACG CCCACTTCTG
    CTTGGTCCAT TTGCTTCACC CAACTTCACG TCTGTGGCCT GTTTGCCTTC AGCTCTGTGC
    CTTCTCTCTG ACCGCAGCCT CTGCCCCAGC CCTCCTCCAC ATCCACCCAT CCTCCCTCTC
    TGGACAACAG CACCTGGATC TCAAGGCTCT CCTCCCCATC ACCAGCCCTT CATCCCACTT
    GCTGTCCCTA TCCTGTGCAG ATGGCTCCCT CACTTGACAC CAGTGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975770.1
    CDS34..5124
    Translation

    Target ORF information:

    RefSeq Version XM_014120295.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA GCTTCACAAG
    ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAAAAG
    AAACTTGAAG ATGAGGCTAT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGGGAA
    AGTCTTAGAA AGGAAGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AGCACAAGAG
    AAAGTTCAAG AAGAGGAGCG GAAAGTGGAG GAGAAACAAT GTGAGCCAGC TAGTGCTTTG
    GTGAATTATA AAGCACTGTA TCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT
    TCCGGGGATA TAATACAGGT TGATGAAAAA ACCATAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCTGTCAGGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCTGT ATCTGCTACC
    TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT
    GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT
    ACTGTGTCCC CTGGATCTAT ATCACCGATT CATGGACAGG GACAAGTTGT AGAAAACCTA
    AAGGCACAGG CCCTTTGTTC TTGGACTGCA AAGAAAGATA ACCACTTGAA CTTTTCAAAA
    CATGATATTA TTACTGTCTT GGAGCAACAA GAAAATTGGT GGTTTGGGGA GGTACATGGA
    GGAAGAGGAT GGTTTCCAAA ATCTTATGTC AAAATCATCC CTGGGAGTGA AGTCAAGCGG
    GAAGAACCAG AAACCTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTATACA
    GTTGGAGAAG AGTATATTGC ACTTTATTCA TATTCAAGTG TAGAACCTGG AGATTTGACT
    TTCACTGAAG GTGAAGAAAT ACTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT
    ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAAT
    TTTGGGAGTG CTAGCAAATC TGGAACGTCA AACAAGAAAC CTGAGATTGC TCAAGTAACT
    TCAGCATATG TAGCTTCTGG TTCTGAACAA CTAAGCCTTG CACCAGGGCA GTTAATATTA
    ATCCTAAAGA AAAATTCAAG TGGATGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG
    CGACAAAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGCGAAAGA
    ACTACACCTG CCTTTCATCC TGTGTGTCAG GTGATTGCTA TGTATGACTA TGCGGCAAAT
    AATGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT
    CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT
    AAGATGACAA CAGACTCTGA TCCAAGTCAA CAGTGGTGCG CGGATCTCCA GAGCCTGGAC
    ACCATGCAGC CAGTGGAGAG GAAGAGGCAG GGCTACATTC ACGAGCTGAT TCAGACTGAA
    GAGCGGTACG TGGATGATCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGCAGAG
    TCAGGGTTTC TCAGTGAAGG AGAGATGGCC TTGATATTTG TAAACTGGAA AGAGCTCATC
    ATGTCTAACA CAAAGCTACT GAAGGCCTTG CGGGTACGGA AGAAGACGGG AGGAGAGAAG
    ATGCCTGTGC AGATGATTGG GGATATCCTG GCAGCTGAGC TGTCCCACAT GCAGGCTTAT
    ATCCGGTTCT GTAGCTGTCA GCTCAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA
    GATGCAGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGGTG TAAAGGAATG
    CCCCTCTCCA GCTTCCTGCT GAAGCCCATG CAGAGGATCA CCCGCTACCC TCTCCTCATC
    AGAAGTATTC TGGAGAACAC CCTGGAGAAT CATGTAGACC ATTCCTCCCT AAAGCTGGCT
    CTTGAGCAAG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT
    TCAGACCGAC TAGAGTGGAT CCAGGCGCAC GTCCAGTGTG AAGGTCTTGC AGAGCAACTT
    ATTTTCAACT CACTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAATTA
    TACAAAACCA AGAGCAACAA GGAGCTACAC GGATTCCTCT TCAACGACTT TCTGCTTCTG
    ACCTACATGG TCAAGCAGTT CGCTGTTTCC TCAGGCTCTG AGAAGCTTTT CAGCTCTAAG
    TCCAATGCCC AGTTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA
    CTGCCCACGG ACCCTCCCAG CGATGAGCCG GTCTTCCACA TTTCTCACAT TGACAGAGTC
    TACACCCTTC GAACAGACAA CATTAACGAG AGGACTGCCT GGGTCCAGAA GATCAAGGCG
    GCATCTGAGC AGTACATCGA TACGGAAAAG AAGAAACGGG AAAAAGCTTA TCAAGGTAGT
    TGGTGGTGCC TGGGGAGTTG GGAGCAGCTG CCAGGGGACC CCAGGGAGGG GCGAGGTCCC
    ACCCTCAGGA CACAAGCTGC TGCTGCCCCT GCAGTGAGGG CACTAAACAG GGACAGCCTG
    AAAGACCAGA CTTGCTGGGG GACCAGACAG CAGAGCCCAC TTGGCTGCTG CCGCCCGTGG
    GAACTCCCTG AGCCCCCTAG CCACTACACG GCGGCGAGGA GCATCCAGTG CCAGCCCCAG
    GACCTCCCTT CTGTCTGCGG CAAAGGAGGA GGGTCCTCTG CAAAACCACG CCCACTTCTG
    CTTGGTCCAT TTGCTTCACC CAACTTCACG TCTGTGGCCT GTTTGCCTTC AGCTCTGTGC
    CTTCTCTCTG ACCGCAGCCT CTGCCCCAGC CCTCCTCCAC ATCCACCCAT CCTCCCTCTC
    TGGACAACAG CACCTGGATC TCAAGGCTCT CCTCCCCATC ACCAGCCCTT CATCCCACTT
    GCTGTCCCTA TCCTGTGCAG ATGGCTCCCT CACTTGACAC CAGTGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40704
    Clone ID Related Accession (Same CDS sequence) XM_022404503.1 , XM_022404504.1 , XM_014120296.2 , XM_014120296.1
    Accession Version XM_022404503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260211.1
    CDS194..5245
    Translation

    Target ORF information:

    RefSeq Version XM_022404503.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40704
    Clone ID Related Accession (Same CDS sequence) XM_022404503.1 , XM_022404504.1 , XM_014120296.2 , XM_014120296.1
    Accession Version XM_022404504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260212.1
    CDS53..5104
    Translation

    Target ORF information:

    RefSeq Version XM_022404504.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40704
    Clone ID Related Accession (Same CDS sequence) XM_022404503.1 , XM_022404504.1 , XM_014120296.2 , XM_014120296.1
    Accession Version XM_014120296.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975771.1
    CDS124..5175
    Translation

    Target ORF information:

    RefSeq Version XM_014120296.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120296.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40704
    Clone ID Related Accession (Same CDS sequence) XM_022404503.1 , XM_022404504.1 , XM_014120296.2 , XM_014120296.1
    Accession Version XM_014120296.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975771.1
    CDS269..5320
    Translation

    Target ORF information:

    RefSeq Version XM_014120296.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGTGTG GGAATATGGA TGACTCTGTT CTGCAGTGCC TTTTGTCTCT GCTAAGCTGT
    CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA
    GCCCTTGAAC AGCTTCACAA GATCAAACGT GACAAGTTGA AGGAAATTGA AAGAAAAAGG
    TCAGAGCTAA TACAGAAAAA GAAACTTGAA GATGAGGCTA TAAGGAAAGC AAAGCAAGGA
    AAAGAAAACT TATGGAGGGA AAGTCTTAGA AAGGAAGAAG AAGAAAAACA AAAGCGACTC
    CAGGAAGAAA AAGCACAAGA GAAAGTTCAA GAAGAGGAGC GGAAAGTGGA GGAGAAACAA
    TGTGAGCCAG CTAGTGCTTT GGTGAATTAT AAAGCACTGT ATCGCTTTGA AGCCAGGAGC
    CATGATGAGA TGAGTTTTAA TTCCGGGGAT ATAATACAGG TTGATGAAAA AACCATAGGA
    GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG GCTGGTTTCC TTGCAACTAT
    GTAGAAAAAA TGCTGTCAGG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCCCCT
    ACAGTATCTG TATCTGCTAC CTCAACTTCT TCTGAATCAC TTTCTTCAAA TCAACCAGCA
    TCAGTGACTG ATTATCAAAA TGTATCTTTT ACAAACCTAA CTGTTAATAC ATCATGGCAG
    AAAAAATCAG CTTTTACTCG TACTGTGTCC CCTGGATCTA TATCACCGAT TCATGGACAG
    GGACAAGTTG TAGAAAACCT AAAGGCACAG GCCCTTTGTT CTTGGACTGC AAAGAAAGAT
    AACCACTTGA ACTTTTCAAA ACATGATATT ATTACTGTCT TGGAGCAACA AGAAAATTGG
    TGGTTTGGGG AGGTACATGG AGGAAGAGGA TGGTTTCCAA AATCTTATGT CAAAATCATC
    CCTGGGAGTG AAGTCAAGCG GGAAGAACCA GAAACCTTGT ATGCAGCTGT AAACAAGAAA
    CCTACCTCTG CAGCCTATAC AGTTGGAGAA GAGTATATTG CACTTTATTC ATATTCAAGT
    GTAGAACCTG GAGATTTGAC TTTCACTGAA GGTGAAGAAA TACTGGTGAC CCAGAAAGAT
    GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAACTGGAA TTTTTCCATC AAATTATGTC
    AAACCAAAAG ATCAAGAGAA TTTTGGGAGT GCTAGCAAAT CTGGAACGTC AAACAAGAAA
    CCTGAGATTG CTCAAGTAAC TTCAGCATAT GTAGCTTCTG GTTCTGAACA ACTAAGCCTT
    GCACCAGGGC AGTTAATATT AATCCTAAAG AAAAATTCAA GTGGATGGTG GCAAGGGGAA
    TTACAGGCCA GAGGAAAAAA GCGACAAAAA GGATGGTTTC CTGCCAGCCA TGTTAAACTT
    TTGGGTCCAA GTAGCGAAAG AACTACACCT GCCTTTCATC CTGTGTGTCA GGTGATTGCT
    ATGTATGACT ATGCGGCAAA TAATGAGGAT GAGCTGAATT TCTCCAAGGG ACAACTTATC
    AATGTTTTGA ACAAAGATGA TCCTGACTGG TGGCAAGGAG AAATCAACGG GGTGACTGGT
    CTCTTTCCTT CAAACTATGT TAAGATGACA ACAGACTCTG ATCCAAGTCA ACAGTGGTGC
    GCGGATCTCC AGAGCCTGGA CACCATGCAG CCAGTGGAGA GGAAGAGGCA GGGCTACATT
    CACGAGCTGA TTCAGACTGA AGAGCGGTAC GTGGATGATC TTCAGCTCGT CGTGGAGGTT
    TTTCAGAAAC GGATGGCAGA GTCAGGGTTT CTCAGTGAAG GAGAGATGGC CTTGATATTT
    GTAAACTGGA AAGAGCTCAT CATGTCTAAC ACAAAGCTAC TGAAGGCCTT GCGGGTACGG
    AAGAAGACGG GAGGAGAGAA GATGCCTGTG CAGATGATTG GGGATATCCT GGCAGCTGAG
    CTGTCCCACA TGCAGGCTTA TATCCGGTTC TGTAGCTGTC AGCTCAATGG AGCAGCTCTG
    TTACAGCAGA AGACAGATGA AGATGCAGAT TTCAAGGAAT TTTTAAAGAA GCTGGCATCT
    GACCCTCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAGCCCAT GCAGAGGATC
    ACCCGCTACC CTCTCCTCAT CAGAAGTATT CTGGAGAACA CCCTGGAGAA TCATGTAGAC
    CATTCCTCCC TAAAGCTGGC TCTTGAGCAA GCAGAGGAGC TTTGTTCCCA AGTTAATGAA
    GGAGTTCGGG AAAAAGAAAA TTCAGACCGA CTAGAGTGGA TCCAGGCGCA CGTCCAGTGT
    GAAGGTCTTG CAGAGCAACT TATTTTCAAC TCACTCACCA ACTGCCTGGG GCCCCGGAAG
    CTTCTGCACA GTGGGAAATT ATACAAAACC AAGAGCAACA AGGAGCTACA CGGATTCCTC
    TTCAACGACT TTCTGCTTCT GACCTACATG GTCAAGCAGT TCGCTGTTTC CTCAGGCTCT
    GAGAAGCTTT TCAGCTCTAA GTCCAATGCC CAGTTCAAAA TGTATAAAAC GCCCATTTTC
    CTGAATGAAG TCTTGGTGAA ACTGCCCACG GACCCTCCCA GCGATGAGCC GGTCTTCCAC
    ATTTCTCACA TTGACAGAGT CTACACCCTT CGAACAGACA ACATTAACGA GAGGACTGCC
    TGGGTCCAGA AGATCAAGGC GGCATCTGAG CAGTACATCG ATACGGAAAA GAAGAAACGG
    GAAAAAGCTT ATCAAGCTCG CTCTCAAAAG ACCTCAGGCA TTGGGCGTCT GATGGTGCAG
    GTCATCGAAG CTACAGAATT AAAAGCCTGT AAACCAAATG GAAAGAGCAA TCCATACTGT
    GAAATCAGCA TGGGCTCCCA GAGCTATACC ACCAGAACCC TCCCGGACAC ACTCAATCCC
    AAATGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGATGT GCTGTGTCTC
    ACCATGTTTG ACAGAGATCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAGTTCCA
    GTGGCAAAAA TTCGCACAGA ACAGGAAAGC AAAGGCCCCA CAACCCGCCG ACTGCTGCTG
    CATGAGGTCC CCACTGGGGA GGTCTGGGTC CGCTTTGACC TGCAGCTTTT TGAGCAAAAA
    ACTCTCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40705
    Clone ID Related Accession (Same CDS sequence) XM_014120297.1 , XM_014120297.2
    Accession Version XM_014120297.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA GCTTCACAAG
    ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAAAAG
    AAACTTGAAG ATGAGGCTAT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGGGAA
    AGTCTTAGAA AGGAAGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AGCACAAGAG
    AAAGTTCAAG AAGAGGAGCG GAAAGTGGAG GAGAAACAAT GTGAGCCAGC TAGTGCTTTG
    GTGAATTATA AAGCACTGTA TCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT
    TCCGGGGATA TAATACAGGT TGATGAAAAA ACCATAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCTGTCAGGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCTGT ATCTGCTACC
    TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT
    GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT
    ACTGTGTCCC CTGGATCTAT ATCACCGATT CATGGACAGG GACAAGTTGT AGAAAACCTA
    AAGGCACAGG CCCTTTGTTC TTGGACTGCA AAGAAAGATA ACCACTTGAA CTTTTCAAAA
    CATGATATTA TTACTGTCTT GGAGCAACAA GAAAATTGGT GGTTTGGGGA GGTACATGGA
    GGAAGAGGAT GGTTTCCAAA ATCTTATGTC AAAATCATCC CTGGGAGTGA AGTCAAGCGG
    GAAGAACCAG AAACCTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTATACA
    GTTGGAGAAG AGTATATTGC ACTTTATTCA TATTCAAGTG TAGAACCTGG AGATTTGACT
    TTCACTGAAG GTGAAGAAAT ACTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT
    ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAAT
    TTTGGGAGTG CTAGCAAATC TGGAACGTCA AACAAGAAAC CTGAGATTGC TCAAGTAACT
    TCAGCATATG TAGCTTCTGG TTCTGAACAA CTAAGCCTTG CACCAGGGCA GTTAATATTA
    ATCCTAAAGA AAAATTCAAG TGGATGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG
    CGACAAAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGCGAAAGA
    ACTACACCTG CCTTTCATCC TGTGTGTCAG GTGATTGCTA TGTATGACTA TGCGGCAAAT
    AATGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT
    CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT
    AAGATGACAA CAGACTCTGA TCCAAGTCAA CAGTGGTGCG CGGATCTCCA GAGCCTGGAC
    ACCATGCAGC CAGTGGAGAG GAAGAGGCAG GGCTACATTC ACGAGCTGAT TCAGACTGAA
    GAGCGGTACG TGGATGATCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGCAGAG
    TCAGGGTTTC TCAGTGAAGG AGAGATGGCC TTGATATTTG TAAACTGGAA AGAGCTCATC
    ATGTCTAACA CAAAGCTACT GAAGGCCTTG CGGGTACGGA AGAAGACGGG AGGAGAGAAG
    ATGCCTGTGC AGATGATTGG GGATATCCTG GCAGCTGAGC TGTCCCACAT GCAGGCTTAT
    ATCCGGTTCT GTAGCTGTCA GCTCAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA
    GATGCAGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGGTG TAAAGGAATG
    CCCCTCTCCA GCTTCCTGCT GAAGCCCATG CAGAGGATCA CCCGCTACCC TCTCCTCATC
    AGAAGTATTC TGGAGAACAC CCTGGAGAAT CATGTAGACC ATTCCTCCCT AAAGCTGGCT
    CTTGAGCAAG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT
    TCAGACCGAC TAGAGTGGAT CCAGGCGCAC GTCCAGTGTG AAGGTCTTGC AGAGCAACTT
    ATTTTCAACT CACTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAATTA
    TACAAAACCA AGAGCAACAA GGAGCTACAC GGATTCCTCT TCAACGACTT TCTGCTTCTG
    ACCTACATGG TCAAGCAGTT CGCTGTTTCC TCAGGCTCTG AGAAGCTTTT CAGCTCTAAG
    TCCAATGCCC AGTTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA
    CTGCCCACGG ACCCTCCCAG CGATGAGCCG GTCTTCCACA TTTCTCACAT TGACAGAGTC
    TACACCCTTC GAACAGACAA CATTAACGAG AGGACTGCCT GGGTCCAGAA GATCAAGGCG
    GCATCTGAGC AGTACATCGA TACGGAAAAG AAGAAACGGG AAAAAGCTTA TCAAGCTCGC
    TCTCAAAAGA CCTCAGGCAT TGGGCGTCTG ATGGTGCAGG TCATCGAAGC TACAGAATTA
    AAAGCCTGTA AACCAAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG
    AGCTATACCA CCAGAACCCT CCCGGACACA CTCAATCCCA AATGGAATTT TAACTGCCAG
    TTCTTTATTA AGGATCTCTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGATCAG
    TTTTCACCAG ATGATTTCCT GGGTCGTACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA
    CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTGCTGCTGC ATGAGGTCCC CACTGGGGAG
    GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975772.1
    CDS34..5004
    Translation

    Target ORF information:

    RefSeq Version XM_014120297.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA GCTTCACAAG
    ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAAAAG
    AAACTTGAAG ATGAGGCTAT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGGGAA
    AGTCTTAGAA AGGAAGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AGCACAAGAG
    AAAGTTCAAG AAGAGGAGCG GAAAGTGGAG GAGAAACAAT GTGAGCCAGC TAGTGCTTTG
    GTGAATTATA AAGCACTGTA TCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT
    TCCGGGGATA TAATACAGGT TGATGAAAAA ACCATAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCTGTCAGGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCTGT ATCTGCTACC
    TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT
    GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT
    ACTGTGTCCC CTGGATCTAT ATCACCGATT CATGGACAGG GACAAGTTGT AGAAAACCTA
    AAGGCACAGG CCCTTTGTTC TTGGACTGCA AAGAAAGATA ACCACTTGAA CTTTTCAAAA
    CATGATATTA TTACTGTCTT GGAGCAACAA GAAAATTGGT GGTTTGGGGA GGTACATGGA
    GGAAGAGGAT GGTTTCCAAA ATCTTATGTC AAAATCATCC CTGGGAGTGA AGTCAAGCGG
    GAAGAACCAG AAACCTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTATACA
    GTTGGAGAAG AGTATATTGC ACTTTATTCA TATTCAAGTG TAGAACCTGG AGATTTGACT
    TTCACTGAAG GTGAAGAAAT ACTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT
    ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAAT
    TTTGGGAGTG CTAGCAAATC TGGAACGTCA AACAAGAAAC CTGAGATTGC TCAAGTAACT
    TCAGCATATG TAGCTTCTGG TTCTGAACAA CTAAGCCTTG CACCAGGGCA GTTAATATTA
    ATCCTAAAGA AAAATTCAAG TGGATGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG
    CGACAAAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGCGAAAGA
    ACTACACCTG CCTTTCATCC TGTGTGTCAG GTGATTGCTA TGTATGACTA TGCGGCAAAT
    AATGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT
    CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT
    AAGATGACAA CAGACTCTGA TCCAAGTCAA CAGTGGTGCG CGGATCTCCA GAGCCTGGAC
    ACCATGCAGC CAGTGGAGAG GAAGAGGCAG GGCTACATTC ACGAGCTGAT TCAGACTGAA
    GAGCGGTACG TGGATGATCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGCAGAG
    TCAGGGTTTC TCAGTGAAGG AGAGATGGCC TTGATATTTG TAAACTGGAA AGAGCTCATC
    ATGTCTAACA CAAAGCTACT GAAGGCCTTG CGGGTACGGA AGAAGACGGG AGGAGAGAAG
    ATGCCTGTGC AGATGATTGG GGATATCCTG GCAGCTGAGC TGTCCCACAT GCAGGCTTAT
    ATCCGGTTCT GTAGCTGTCA GCTCAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA
    GATGCAGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGGTG TAAAGGAATG
    CCCCTCTCCA GCTTCCTGCT GAAGCCCATG CAGAGGATCA CCCGCTACCC TCTCCTCATC
    AGAAGTATTC TGGAGAACAC CCTGGAGAAT CATGTAGACC ATTCCTCCCT AAAGCTGGCT
    CTTGAGCAAG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT
    TCAGACCGAC TAGAGTGGAT CCAGGCGCAC GTCCAGTGTG AAGGTCTTGC AGAGCAACTT
    ATTTTCAACT CACTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAATTA
    TACAAAACCA AGAGCAACAA GGAGCTACAC GGATTCCTCT TCAACGACTT TCTGCTTCTG
    ACCTACATGG TCAAGCAGTT CGCTGTTTCC TCAGGCTCTG AGAAGCTTTT CAGCTCTAAG
    TCCAATGCCC AGTTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA
    CTGCCCACGG ACCCTCCCAG CGATGAGCCG GTCTTCCACA TTTCTCACAT TGACAGAGTC
    TACACCCTTC GAACAGACAA CATTAACGAG AGGACTGCCT GGGTCCAGAA GATCAAGGCG
    GCATCTGAGC AGTACATCGA TACGGAAAAG AAGAAACGGG AAAAAGCTTA TCAAGCTCGC
    TCTCAAAAGA CCTCAGGCAT TGGGCGTCTG ATGGTGCAGG TCATCGAAGC TACAGAATTA
    AAAGCCTGTA AACCAAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG
    AGCTATACCA CCAGAACCCT CCCGGACACA CTCAATCCCA AATGGAATTT TAACTGCCAG
    TTCTTTATTA AGGATCTCTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGATCAG
    TTTTCACCAG ATGATTTCCT GGGTCGTACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA
    CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTGCTGCTGC ATGAGGTCCC CACTGGGGAG
    GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40705
    Clone ID Related Accession (Same CDS sequence) XM_014120297.1 , XM_014120297.2
    Accession Version XM_014120297.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA GCTTCACAAG
    ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAAAAG
    AAACTTGAAG ATGAGGCTAT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGGGAA
    AGTCTTAGAA AGGAAGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AGCACAAGAG
    AAAGTTCAAG AAGAGGAGCG GAAAGTGGAG GAGAAACAAT GTGAGCCAGC TAGTGCTTTG
    GTGAATTATA AAGCACTGTA TCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT
    TCCGGGGATA TAATACAGGT TGATGAAAAA ACCATAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCTGTCAGGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCTGT ATCTGCTACC
    TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT
    GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT
    ACTGTGTCCC CTGGATCTAT ATCACCGATT CATGGACAGG GACAAGTTGT AGAAAACCTA
    AAGGCACAGG CCCTTTGTTC TTGGACTGCA AAGAAAGATA ACCACTTGAA CTTTTCAAAA
    CATGATATTA TTACTGTCTT GGAGCAACAA GAAAATTGGT GGTTTGGGGA GGTACATGGA
    GGAAGAGGAT GGTTTCCAAA ATCTTATGTC AAAATCATCC CTGGGAGTGA AGTCAAGCGG
    GAAGAACCAG AAACCTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTATACA
    GTTGGAGAAG AGTATATTGC ACTTTATTCA TATTCAAGTG TAGAACCTGG AGATTTGACT
    TTCACTGAAG GTGAAGAAAT ACTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT
    ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAAT
    TTTGGGAGTG CTAGCAAATC TGGAACGTCA AACAAGAAAC CTGAGATTGC TCAAGTAACT
    TCAGCATATG TAGCTTCTGG TTCTGAACAA CTAAGCCTTG CACCAGGGCA GTTAATATTA
    ATCCTAAAGA AAAATTCAAG TGGATGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG
    CGACAAAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGCGAAAGA
    ACTACACCTG CCTTTCATCC TGTGTGTCAG GTGATTGCTA TGTATGACTA TGCGGCAAAT
    AATGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT
    CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT
    AAGATGACAA CAGACTCTGA TCCAAGTCAA CAGTGGTGCG CGGATCTCCA GAGCCTGGAC
    ACCATGCAGC CAGTGGAGAG GAAGAGGCAG GGCTACATTC ACGAGCTGAT TCAGACTGAA
    GAGCGGTACG TGGATGATCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGCAGAG
    TCAGGGTTTC TCAGTGAAGG AGAGATGGCC TTGATATTTG TAAACTGGAA AGAGCTCATC
    ATGTCTAACA CAAAGCTACT GAAGGCCTTG CGGGTACGGA AGAAGACGGG AGGAGAGAAG
    ATGCCTGTGC AGATGATTGG GGATATCCTG GCAGCTGAGC TGTCCCACAT GCAGGCTTAT
    ATCCGGTTCT GTAGCTGTCA GCTCAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA
    GATGCAGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGGTG TAAAGGAATG
    CCCCTCTCCA GCTTCCTGCT GAAGCCCATG CAGAGGATCA CCCGCTACCC TCTCCTCATC
    AGAAGTATTC TGGAGAACAC CCTGGAGAAT CATGTAGACC ATTCCTCCCT AAAGCTGGCT
    CTTGAGCAAG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT
    TCAGACCGAC TAGAGTGGAT CCAGGCGCAC GTCCAGTGTG AAGGTCTTGC AGAGCAACTT
    ATTTTCAACT CACTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAATTA
    TACAAAACCA AGAGCAACAA GGAGCTACAC GGATTCCTCT TCAACGACTT TCTGCTTCTG
    ACCTACATGG TCAAGCAGTT CGCTGTTTCC TCAGGCTCTG AGAAGCTTTT CAGCTCTAAG
    TCCAATGCCC AGTTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA
    CTGCCCACGG ACCCTCCCAG CGATGAGCCG GTCTTCCACA TTTCTCACAT TGACAGAGTC
    TACACCCTTC GAACAGACAA CATTAACGAG AGGACTGCCT GGGTCCAGAA GATCAAGGCG
    GCATCTGAGC AGTACATCGA TACGGAAAAG AAGAAACGGG AAAAAGCTTA TCAAGCTCGC
    TCTCAAAAGA CCTCAGGCAT TGGGCGTCTG ATGGTGCAGG TCATCGAAGC TACAGAATTA
    AAAGCCTGTA AACCAAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG
    AGCTATACCA CCAGAACCCT CCCGGACACA CTCAATCCCA AATGGAATTT TAACTGCCAG
    TTCTTTATTA AGGATCTCTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGATCAG
    TTTTCACCAG ATGATTTCCT GGGTCGTACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA
    CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTGCTGCTGC ATGAGGTCCC CACTGGGGAG
    GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975772.1
    CDS34..5004
    Translation

    Target ORF information:

    RefSeq Version XM_014120297.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120297.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
    GAAGAACGGA CTAAGCATGA CAAGCAATTT GATAACCTCA AACCCTCAGG AGGTTATATA
    ACAGGGGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC TGTTTTAGCT
    GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAGCA GGAGTTCTCC
    ATAGCAATGA AACTCATCAA ACTAAAGCTG CAAGGCCAAC AGTTGCCCGT AGTCCTCCCT
    CCTATTATGA AACAACCTCC TATGTTCTCT CCTTTAATTT CTGCTCGTTT TGGAATGGGA
    AGCATGCCCA ATCTGTCCAT TCCTCACTCA TTGCCTCCAG TTGCACCTAT GACAACCCCC
    TTATCCTCTG CGACTTCAGG GACCACCCTT CCTCCCGTAA TGATGCCGGC TCCCCTAGTG
    CCTTCTGTTA GCACATCATC ACTACCAAAT GGAACTCCTG GCCTCATTCA GCCTTTATCC
    ATTCCTTACT CTTCTTCAAC ATTGCCTCAT ACATCATCTT ACAGTCTCAT GATGGGAGGA
    CTCGGCGGTG CTAGTTTACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA
    ACGTCCTCAA CTGCTTCACT ATCTGGGAAC TCGCCCAAGA CTGGGACCTC AGAGTGGGCA
    GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ATAGTCTTGA CAAAAGCATG
    AGTGGGTACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTTTCTCAA
    ACTCAGCTAG CTACTATTTG GACTCTGGCT GATATTGATG GGGACGGACA GCTGAAAGCT
    GAAGAATTCA TCCTTGCAAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCGTTGCCA
    CTGACGTTAC CTCCTGAGCT TGTCCCTCCG TCTTTCAGAG GAGGAAAGCA GATTGATTCT
    ATTAATGGAA CTCTGCCTTC ATATCAGAAA ATGCAAGAAG AGGAACCTCA GAAAAAATTA
    CCAGTTACTT TCGAGGACAA GCGGAAGGCC AACTACGAGC GAGGGAATGT GGAACTGGAG
    AAGCGGCGCC AAGCCTTGAT GGAGCAGCAG CAGAGGGAGG CAGAACGTAA AGCCCAGAAA
    GAAAAGGAAG AGTGGGAGCG AAAACAGAGA GAATTACAGG AGCAAGAATG GAAGAAACAA
    CTCGAATTAG AAAAACGCTT GGAGAAGCAA AGGGAATTGG AGAGACAGCG GGAGGAAGAA
    AGGAGAAAAG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AACTTGAAAG ACAACGTCGT
    TTAGAATGGG AGAGGATTCG TCGACAAGAG CTTCTCAATC AAAGGAATAG AGAACAGGAA
    GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGTCTTCATC TTGAGCTGGA AGCGCTGAAT
    GGCAAACATC AGCAGATCTC AGGCAGACTT CAAGATGTCC GACTCCGAAA ACAAACTCAA
    AAGACTGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATTATGGA AATCAAGCAA
    CTCCAACAAG AACTTCAGGA TTATCAAAAT AAGCTTATCT ATTTGGTACC TGAGAAGCAA
    TTATTAAATG AAAGAATTAA GAACATGCAA CTTGGTAACA CACCTGATTT AGGGATTGGT
    TTGCTTCATA AAAAATCATT GGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA
    GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTATCTGAAA TGGATTCATT TAACAACCAA
    CTAAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCCTTGAACA GCTTCACAAG
    ATCAAACGTG ACAAGTTGAA GGAAATTGAA AGAAAAAGGT CAGAGCTAAT ACAGAAAAAG
    AAACTTGAAG ATGAGGCTAT AAGGAAAGCA AAGCAAGGAA AAGAAAACTT ATGGAGGGAA
    AGTCTTAGAA AGGAAGAAGA AGAAAAACAA AAGCGACTCC AGGAAGAAAA AGCACAAGAG
    AAAGTTCAAG AAGAGGAGCG GAAAGTGGAG GAGAAACAAT GTGAGCCAGC TAGTGCTTTG
    GTGAATTATA AAGCACTGTA TCGCTTTGAA GCCAGGAGCC ATGATGAGAT GAGTTTTAAT
    TCCGGGGATA TAATACAGGT TGATGAAAAA ACCATAGGAG AACCTGGTTG GCTTTATGGT
    AGTTTTCAAG GAAATTTTGG CTGGTTTCCT TGCAACTATG TAGAAAAAAT GCTGTCAGGT
    GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCCCCTA CAGTATCTGT ATCTGCTACC
    TCAACTTCTT CTGAATCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT
    GTATCTTTTA CAAACCTAAC TGTTAATACA TCATGGCAGA AAAAATCAGC TTTTACTCGT
    ACTGTGTCCC CTGGATCTAT ATCACCGATT CATGGACAGG GACAAGTTGT AGAAAACCTA
    AAGGCACAGG CCCTTTGTTC TTGGACTGCA AAGAAAGATA ACCACTTGAA CTTTTCAAAA
    CATGATATTA TTACTGTCTT GGAGCAACAA GAAAATTGGT GGTTTGGGGA GGTACATGGA
    GGAAGAGGAT GGTTTCCAAA ATCTTATGTC AAAATCATCC CTGGGAGTGA AGTCAAGCGG
    GAAGAACCAG AAACCTTGTA TGCAGCTGTA AACAAGAAAC CTACCTCTGC AGCCTATACA
    GTTGGAGAAG AGTATATTGC ACTTTATTCA TATTCAAGTG TAGAACCTGG AGATTTGACT
    TTCACTGAAG GTGAAGAAAT ACTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT
    ATTGGAGATA GAACTGGAAT TTTTCCATCA AATTATGTCA AACCAAAAGA TCAAGAGAAT
    TTTGGGAGTG CTAGCAAATC TGGAACGTCA AACAAGAAAC CTGAGATTGC TCAAGTAACT
    TCAGCATATG TAGCTTCTGG TTCTGAACAA CTAAGCCTTG CACCAGGGCA GTTAATATTA
    ATCCTAAAGA AAAATTCAAG TGGATGGTGG CAAGGGGAAT TACAGGCCAG AGGAAAAAAG
    CGACAAAAAG GATGGTTTCC TGCCAGCCAT GTTAAACTTT TGGGTCCAAG TAGCGAAAGA
    ACTACACCTG CCTTTCATCC TGTGTGTCAG GTGATTGCTA TGTATGACTA TGCGGCAAAT
    AATGAGGATG AGCTGAATTT CTCCAAGGGA CAACTTATCA ATGTTTTGAA CAAAGATGAT
    CCTGACTGGT GGCAAGGAGA AATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTATGTT
    AAGATGACAA CAGACTCTGA TCCAAGTCAA CAGTGGTGCG CGGATCTCCA GAGCCTGGAC
    ACCATGCAGC CAGTGGAGAG GAAGAGGCAG GGCTACATTC ACGAGCTGAT TCAGACTGAA
    GAGCGGTACG TGGATGATCT TCAGCTCGTC GTGGAGGTTT TTCAGAAACG GATGGCAGAG
    TCAGGGTTTC TCAGTGAAGG AGAGATGGCC TTGATATTTG TAAACTGGAA AGAGCTCATC
    ATGTCTAACA CAAAGCTACT GAAGGCCTTG CGGGTACGGA AGAAGACGGG AGGAGAGAAG
    ATGCCTGTGC AGATGATTGG GGATATCCTG GCAGCTGAGC TGTCCCACAT GCAGGCTTAT
    ATCCGGTTCT GTAGCTGTCA GCTCAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA
    GATGCAGATT TCAAGGAATT TTTAAAGAAG CTGGCATCTG ACCCTCGGTG TAAAGGAATG
    CCCCTCTCCA GCTTCCTGCT GAAGCCCATG CAGAGGATCA CCCGCTACCC TCTCCTCATC
    AGAAGTATTC TGGAGAACAC CCTGGAGAAT CATGTAGACC ATTCCTCCCT AAAGCTGGCT
    CTTGAGCAAG CAGAGGAGCT TTGTTCCCAA GTTAATGAAG GAGTTCGGGA AAAAGAAAAT
    TCAGACCGAC TAGAGTGGAT CCAGGCGCAC GTCCAGTGTG AAGGTCTTGC AGAGCAACTT
    ATTTTCAACT CACTCACCAA CTGCCTGGGG CCCCGGAAGC TTCTGCACAG TGGGAAATTA
    TACAAAACCA AGAGCAACAA GGAGCTACAC GGATTCCTCT TCAACGACTT TCTGCTTCTG
    ACCTACATGG TCAAGCAGTT CGCTGTTTCC TCAGGCTCTG AGAAGCTTTT CAGCTCTAAG
    TCCAATGCCC AGTTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA
    CTGCCCACGG ACCCTCCCAG CGATGAGCCG GTCTTCCACA TTTCTCACAT TGACAGAGTC
    TACACCCTTC GAACAGACAA CATTAACGAG AGGACTGCCT GGGTCCAGAA GATCAAGGCG
    GCATCTGAGC AGTACATCGA TACGGAAAAG AAGAAACGGG AAAAAGCTTA TCAAGCTCGC
    TCTCAAAAGA CCTCAGGCAT TGGGCGTCTG ATGGTGCAGG TCATCGAAGC TACAGAATTA
    AAAGCCTGTA AACCAAATGG AAAGAGCAAT CCATACTGTG AAATCAGCAT GGGCTCCCAG
    AGCTATACCA CCAGAACCCT CCCGGACACA CTCAATCCCA AATGGAATTT TAACTGCCAG
    TTCTTTATTA AGGATCTCTA CCAAGATGTG CTGTGTCTCA CCATGTTTGA CAGAGATCAG
    TTTTCACCAG ATGATTTCCT GGGTCGTACT GAAGTTCCAG TGGCAAAAAT TCGCACAGAA
    CAGGAAAGCA AAGGCCCCAC AACCCGCCGA CTGCTGCTGC ATGAGGTCCC CACTGGGGAG
    GTCTGGGTCC GCTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40706
    Clone ID Related Accession (Same CDS sequence) XM_014120298.2 , XM_014120298.1
    Accession Version XM_014120298.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    TTCCCTAATT TTGTTATATA CTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975773.1
    CDS194..4960
    Translation

    Target ORF information:

    RefSeq Version XM_014120298.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120298.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    TTCCCTAATT TTGTTATATA CTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40706
    Clone ID Related Accession (Same CDS sequence) XM_014120298.2 , XM_014120298.1
    Accession Version XM_014120298.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    TTCCCTAATT TTGTTATATA CTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975773.1
    CDS176..4942
    Translation

    Target ORF information:

    RefSeq Version XM_014120298.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 2 (ITSN2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGGCTC AGTTTCCCAC AGCTATGAAT GAGCAAAAAA GATCAGTGCG TCATATTTTT 
    ACCACTCGTG ACTCTAATTC TGGTGTCTTC TGCACCAGCA GAATCATCCT TGCCAGAGTC
    TTCTTTGATC ATGGAGGGCC AAACATGTGG GCTATTACCT CTGAAGAACG GACTAAGCAT
    GACAAGCAAT TTGATAACCT CAAACCCTCA GGAGGTTATA TAACAGGGGA TCAAGCCCGT
    ACCTTTTTCT TACAGTCAGG CCTGCCGGCT CCTGTTTTAG CTGAAATATG GGCTTTATCA
    GACCTGAACA AGGATGGGAA GATGGATCAG CAGGAGTTCT CCATAGCAAT GAAACTCATC
    AAACTAAAGC TGCAAGGCCA ACAGTTGCCC GTAGTCCTCC CTCCTATTAT GAAACAACCT
    CCTATGTTCT CTCCTTTAAT TTCTGCTCGT TTTGGAATGG GAAGCATGCC CAATCTGTCC
    ATTCCTCACT CATTGCCTCC AGTTGCACCT ATGACAACCC CCTTATCCTC TGCGACTTCA
    GGGACCACCC TTCCTCCCGT AATGATGCCG GCTCCCCTAG TGCCTTCTGT TAGCACATCA
    TCACTACCAA ATGGAACTCC TGGCCTCATT CAGCCTTTAT CCATTCCTTA CTCTTCTTCA
    ACATTGCCTC ATACATCATC TTACAGTCTC ATGATGGGAG GACTCGGCGG TGCTAGTTTA
    CAGAAAGCCC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT CAACGTCCTC AACTGCTTCA
    CTATCTGGGA ACTCGCCCAA GACTGGGACC TCAGAGTGGG CAGTTCCTCA GCCTTCAAGA
    TTAAAATATC GGCAAAAATT TAATAGTCTT GACAAAAGCA TGAGTGGGTA CCTCTCAGGT
    TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC AAACTCAGCT AGCTACTATT
    TGGACTCTGG CTGATATTGA TGGGGACGGA CAGCTGAAAG CTGAAGAATT CATCCTTGCA
    ATGCACCTCA CTGACATGGC CAAAGCTGGA CAGCCGTTGC CACTGACGTT ACCTCCTGAG
    CTTGTCCCTC CGTCTTTCAG AGGAGGAAAG CAGATTGATT CTATTAATGG AACTCTGCCT
    TCATATCAGA AAATGCAAGA AGAGGAACCT CAGAAAAAAT TACCAGTTAC TTTCGAGGAC
    AAGCGGAAGG CCAACTACGA GCGAGGGAAT GTGGAACTGG AGAAGCGGCG CCAAGCCTTG
    ATGGAGCAGC AGCAGAGGGA GGCAGAACGT AAAGCCCAGA AAGAAAAGGA AGAGTGGGAG
    CGAAAACAGA GAGAATTACA GGAGCAAGAA TGGAAGAAAC AACTCGAATT AGAAAAACGC
    TTGGAGAAGC AAAGGGAATT GGAGAGACAG CGGGAGGAAG AAAGGAGAAA AGAGATAGAA
    AGACGAGAGG CAGCAAAACA GGAACTTGAA AGACAACGTC GTTTAGAATG GGAGAGGATT
    CGTCGACAAG AGCTTCTCAA TCAAAGGAAT AGAGAACAGG AAGAAATTGT CAGGTTAAAC
    TCTAAAAAGA AGAGTCTTCA TCTTGAGCTG GAAGCGCTGA ATGGCAAACA TCAGCAGATC
    TCAGGCAGAC TTCAAGATGT CCGACTCCGA AAACAAACTC AAAAGACTGA GCTGGAAGTT
    CTGGATAAGC AGTGTGACCT GGAAATTATG GAAATCAAGC AACTCCAACA AGAACTTCAG
    GATTATCAAA ATAAGCTTAT CTATTTGGTA CCTGAGAAGC AATTATTAAA TGAAAGAATT
    AAGAACATGC AACTTGGTAA CACACCTGAT TTAGGGATTG GTTTGCTTCA TAAAAAATCA
    TTGGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAAT TAGATGCTCT TGAAAAAGAA
    ACTGCATCTA AGCTATCTGA AATGGATTCA TTTAACAACC AACTAAAGTG TGGGAATATG
    GATGACTCTG TTCTGCAGTG CCTTTTGTCT CTGCTAAGCT GTCTCAACAA CCTCTTCCTC
    TTACTTAAGG AACTGAGAGA AAGCTACAAT ACACAGCAGT TAGCCCTTGA ACAGCTTCAC
    AAGATCAAAC GTGACAAGTT GAAGGAAATT GAAAGAAAAA GGTCAGAGCT AATACAGAAA
    AAGAAACTTG AAGATGAGGC TATAAGGAAA GCAAAGCAAG GAAAAGAAAA CTTATGGAGG
    GAAAGTCTTA GAAAGGAAGA AGAAGAAAAA CAAAAGCGAC TCCAGGAAGA AAAAGCACAA
    GAGAAAGTTC AAGAAGAGGA GCGGAAAGTG GAGGAGAAAC AATGTGAGCC AGCTAGTGCT
    TTGGTGAATT ATAAAGCACT GTATCGCTTT GAAGCCAGGA GCCATGATGA GATGAGTTTT
    AATTCCGGGG ATATAATACA GGTTGATGAA AAAACCATAG GAGAACCTGG TTGGCTTTAT
    GGTAGTTTTC AAGGAAATTT TGGCTGGTTT CCTTGCAACT ATGTAGAAAA AATGCTGTCA
    GGTGAAAAAT CTGTGTCTCC TAAGAAGGCC TTACTTCCCC CTACAGTATC TGTATCTGCT
    ACCTCAACTT CTTCTGAATC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA
    AATGTATCTT TTACAAACCT AACTGTTAAT ACATCATGGC AGAAAAAATC AGCTTTTACT
    CGTACTGTGT CCCCTGGATC TATATCACCG ATTCATGGAC AGGGACAAGT TGTAGAAAAC
    CTAAAGGCAC AGGCCCTTTG TTCTTGGACT GCAAAGAAAG ATAACCACTT GAACTTTTCA
    AAACATGATA TTATTACTGT CTTGGAGCAA CAAGAAAATT GGTGGTTTGG GGAGGTACAT
    GGAGGAAGAG GATGGTTTCC AAAATCTTAT GTCAAAATCA TCCCTGGGAG TGAAGTCAAG
    CGGGAAGAAC CAGAAACCTT GTATGCAGCT GTAAACAAGA AACCTACCTC TGCAGCCTAT
    ACAGTTGGAG AAGAGTATAT TGCACTTTAT TCATATTCAA GTGTAGAACC TGGAGATTTG
    ACTTTCACTG AAGGTGAAGA AATACTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA
    AGTATTGGAG ATAGAACTGG AATTTTTCCA TCAAATTATG TCAAACCAAA AGATCAAGAG
    AATTTTGGGA GTGCTAGCAA ATCTGGAACG TCAAACAAGA AACCTGAGAT TGCTCAAGTA
    ACTTCAGCAT ATGTAGCTTC TGGTTCTGAA CAACTAAGCC TTGCACCAGG GCAGTTAATA
    TTAATCCTAA AGAAAAATTC AAGTGGATGG TGGCAAGGGG AATTACAGGC CAGAGGAAAA
    AAGCGACAAA AAGGATGGTT TCCTGCCAGC CATGTTAAAC TTTTGGGTCC AAGTAGCGAA
    AGAACTACAC CTGCCTTTCA TCCTGTGTGT CAGGTGATTG CTATGTATGA CTATGCGGCA
    AATAATGAGG ATGAGCTGAA TTTCTCCAAG GGACAACTTA TCAATGTTTT GAACAAAGAT
    GATCCTGACT GGTGGCAAGG AGAAATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAT
    GTTAAGATGA CAACAGACTC TGATCCAAGT CAACAGTGGT GCGCGGATCT CCAGAGCCTG
    GACACCATGC AGCCAGTGGA GAGGAAGAGG CAGGGCTACA TTCACGAGCT GATTCAGACT
    GAAGAGCGGT ACGTGGATGA TCTTCAGCTC GTCGTGGAGG TTTTTCAGAA ACGGATGGCA
    GAGTCAGGGT TTCTCAGTGA AGGAGAGATG GCCTTGATAT TTGTAAACTG GAAAGAGCTC
    ATCATGTCTA ACACAAAGCT ACTGAAGGCC TTGCGGGTAC GGAAGAAGAC GGGAGGAGAG
    AAGATGCCTG TGCAGATGAT TGGGGATATC CTGGCAGCTG AGCTGTCCCA CATGCAGGCT
    TATATCCGGT TCTGTAGCTG TCAGCTCAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT
    GAAGATGCAG ATTTCAAGGA ATTTTTAAAG AAGCTGGCAT CTGACCCTCG GTGTAAAGGA
    ATGCCCCTCT CCAGCTTCCT GCTGAAGCCC ATGCAGAGGA TCACCCGCTA CCCTCTCCTC
    ATCAGAAGTA TTCTGGAGAA CACCCTGGAG AATCATGTAG ACCATTCCTC CCTAAAGCTG
    GCTCTTGAGC AAGCAGAGGA GCTTTGTTCC CAAGTTAATG AAGGAGTTCG GGAAAAAGAA
    AATTCAGACC GACTAGAGTG GATCCAGGCG CACGTCCAGT GTGAAGGTCT TGCAGAGCAA
    CTTATTTTCA ACTCACTCAC CAACTGCCTG GGGCCCCGGA AGCTTCTGCA CAGTGGGAAA
    TTATACAAAA CCAAGAGCAA CAAGGAGCTA CACGGATTCC TCTTCAACGA CTTTCTGCTT
    CTGACCTACA TGGTCAAGCA GTTCGCTGTT TCCTCAGGCT CTGAGAAGCT TTTCAGCTCT
    AAGTCCAATG CCCAGTTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG
    AAACTGCCCA CGGACCCTCC CAGCGATGAG CCGGTCTTCC ACATTTCTCA CATTGACAGA
    GTCTACACCC TTCGAACAGA CAACATTAAC GAGAGGACTG CCTGGGTCCA GAAGATCAAG
    GCGGCATCTG AGCAGTACAT CGATACGGAA AAGAAGAAAC GGGAAAAAGC TTATCAAGGT
    TTCCCTAATT TTGTTATATA CTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.