gRNAs
The following ADAM17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAM17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OCe00441 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001286866.1 | |
| Accession Version | NM_001286866.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-22 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform 1 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010702.1 and AAEX03010701.1. On Nov 19, 2013 this sequence version replaced XM_005630109.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB793308.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGGCGGT GTGTCCTCTT CTGGACCAGC GTGGTTCCCT TGGTGCTGGC GCCGCGACCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001273795.1 |
| CDS | 150..2621 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001286866.1 |
1 | ATGAGGCGGT GTGTCCTCTT CTGGACCAGC GTGGTTCCCT TGGTGCTGGC GCCGCGACCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe33700 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001286867.1 | |
| Accession Version | NM_001286867.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-22 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010702.1 and AAEX03010701.1. Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB793306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGCAGAG GGGAAGAAAG TACAACTACA AATTACTTAA TAGAGCTTAT TGACCGGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001273796.1 |
| CDS | 872..2641 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001286867.1 |
1 | ATGGGCAGAG GGGAAGAAAG TACAACTACA AATTACTTAA TAGAGCTTAT TGACCGGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe33701 | |
| Clone ID Related Accession (Same CDS sequence) | XM_022404326.1 , NM_001286868.1 | |
| Accession Version | NM_001286868.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-22 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010702.1 and AAEX03010701.1. Transcript Variant: This variant (3) includes an alternate internal exon resulting in initiation of translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB793305.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001273797.1 |
| CDS | 1105..2691 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001286868.1 |
1 | ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe18617 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630110.3 , XM_005630110.2 | |
| Accession Version | XM_005630110.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630110.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACGATAA GAATCAACAC AGATGGAGCA GAATATAATA TAGAGCCACT TTGGAGACTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630167.1 |
| CDS | 467..2545 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630110.3 |
1 | ATGACGATAA GAATCAACAC AGATGGAGCA GAATATAATA TAGAGCCACT TTGGAGACTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe18617 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630110.3 , XM_005630110.2 | |
| Accession Version | XM_005630110.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACGATAA GAATCAACAC AGATGGAGCA GAATATAATA TAGAGCCACT TTGGAGACTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630167.1 |
| CDS | 257..2335 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630110.2 |
1 | ATGACGATAA GAATCAACAC AGATGGAGCA GAATATAATA TAGAGCCACT TTGGAGACTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe294146 | |
| Clone ID Related Accession (Same CDS sequence) | XM_022404325.1 | |
| Accession Version | XM_022404325.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAATGGGA GAGGGATACT GGAAGGGGCC CTCGGGGGGG GACTGACGGC CTGGGGGTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_022260033.1 |
| CDS | 16..2490 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_022404325.1 |
1 | ATGAATGGGA GAGGGATACT GGAAGGGGCC CTCGGGGGGG GACTGACGGC CTGGGGGTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe33701 | |
| Clone ID Related Accession (Same CDS sequence) | XM_022404326.1 , NM_001286868.1 | |
| Accession Version | XM_022404326.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | disintegrin and metalloproteinase domain-containing protein 17 isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_022260034.1 |
| CDS | 150..1736 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_022404326.1 |
1 | ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing. |
