DLC1 cDNA ORF clone, Canis lupus familiaris(dog)

The following DLC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DLC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe00250 NM_001145071.1
Latest version!
Canis lupus familiaris DLC1 Rho GTPase activating protein (DLC1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe155266 NM_001313797.1
Latest version!
Canis lupus familiaris DLC1 Rho GTPase activating protein (DLC1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe00250
    Clone ID Related Accession (Same CDS sequence) NM_001145071.1
    Accession Version NM_001145071.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Canis lupus familiaris(dog)
    Product rho GTPase-activating protein 7 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FJ602870.1. On Feb 9, 2009 this sequence version replaced XM_532822.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: FJ602870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN07419819 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTGCAGGA GGAAGCCGGA CATCATGATC CTAACACAAA TTGAAGCCAA GGAAGCTTGT 
    GATTGGCTAC GGGCAACAGG TTTCCCCCAG TATGCACAGC TTTACGAAGA TCTTCTGTTC
    CCCATTGATA TTTCCTCGGT CAAGAGAGAG CATGACTTTT TGGACAGAGA TGCCATTGAG
    GCTTTATGCA GGCGTCTAAA TACTCTAAAC AAATGTGCGG TGATGAAGCT GGAAATTAGT
    CCTCATCGGA AGAGAAGTGA GGATTCGGAT GAGGACGAGC CTTGTGCAAT AAGCGGCAAG
    TGGACCTTCC AGAGAGACAG CAAGAGGTGG TCACGGCTGG AGGAGTTTGA TGTCTTTTCT
    TCAAAGCTGG ACCCAGCCCC CGGGGCGCCC GCCGAGGCCC CCCTGAAGAC CGCGGCGAGC
    CACGAAAGCA TGCTGACAGA GCTCAGCGAG CGCCAGGAGG TGGCTTCGGT CCTCAGCCTC
    AGCAGCACCG GCAGTCTCCC CGTGCACGCG CCCCACGCGG GGGACGCGGC AACGCCCCGG
    ACCAACTCGG TCATCAGCGT CTGCTCCTCC GGCCACTTCG TGGGCAACGA CGACTCCTTC
    TGCAGCCTGC CCTCTCCCAA GGAACTGTCC AGCTTCAGCT TCAGCATGAA AGGCCAAGAG
    AAGAACGCCA AGTCCAAGAC GCGCAGCCTG CTGAAGCGCA TGGAGAGCCT GAAGCTCAGG
    GGCTCGCCCC ACAGCAAGCA CAAGGCGCCT TCCAAGCTGG GGCTGATCAT CAGCGGGCCC
    ATCCTGCAGG AGGGGATGGA CGAGGAGAAG CTGAAGCAGC TCAACTGTGT GGAGATCTCC
    GCGCTCAACG GCAACCGCAT CAACGTGCCG GCCGTGCGCA AGCGGAGCGT CTCCAACTCC
    ACGCAGACCA GCAGCAGCAG CAGCCAGTCG GAGACCAGCA GCGCCGTGAG CACGCCCAGC
    CCGGTCACCA GGACTCGGAG CCTCAGTGCC TGCAACAAGC GGGTGGGCAT GTACTTAGAG
    GGCTTTGACC CGTTCAATCA GTCCACGTTC AACAACGTTA TGGAGCAGAA CTGTAAGAAC
    CGCGAGAGCT ACCCAGAGGA CACAGTGTTC TACATCCCAG AAGATCACAA GCCTGGCACT
    TTCCCCAAGG CCCTCTCCAA TGGCAGTTTC CCTCCCTCAG GGAATAACAG CTCTGTCAAC
    TGGAGGACTG GAAGCTTCCA CGGCCCCGGC CACATCAGCC TGAGGAGGGA AAACAGCAGT
    GACAGCCCCA AGGAGCTCAA GAGGCGCAAT TCCTCCAGTT CCATGAGCAG CCGCCTGAGT
    ATCTATGACA ACGTGCCAGG CTCCATCCTC TATTCCAGTT CAGGGGACCT GGCCGACCTG
    GAGAACGAGG ACATCTTCCC CGAGCTGGAT GACATCCTCT ACCATGTGAA GGGGATGCAA
    AGGATAGTCA ACCAGTGGTC CGAGAAGTTT TCAGACGAGG GGGACTCTGA CTCAGCCCTG
    GACTCGGTCT CTCCATGCCC CTCCTCTCCC AAACAGATAC ACCTGGATGT GGACAACGAC
    CGAGCCACGC CCAGTGACCT AGACAGTACG GGCAACTCAC TGAATGAACC AGAAGAGCCC
    TCAGACATCC CGGAGAGGAG GGATTCTGGG GTTGGGGCCT CCCTGACAAG GTCCAACAGG
    CACAGGCTGA GATGGCACAG TTTCCAGAGC TCCCATCGGC CGAGCTTAAA CTCTGTATCC
    CTGCAGATCA ACTGCCAGTC TGTGGCCCAG ATGAACCTGC TGCAGAAATA CTCGCTCCTA
    AAGTTAACTG CCCTGCTGGA GAAATACACA CCCTCTAATA AGCATGGTTT TAGCTGGGCT
    GTGCCCAAGT TCATGAAAAG GATCAAGGTT CCAGACTACA AGGACCGGAA TGTATTCGGG
    GTCCCTCTGA CAGTCAATGT GCAGCGCACA GGACAGCCCC TGCCCCAGAG CATTCAGCAG
    GCCATGCGCT ACCTCCGCAA CCACTGTTTG GATCAGGTTG GGCTCTTCAG AAAATCAGGC
    GTCAAATCCC GAATTCAGGC TCTGCGCCAG ATGAATGAAA GTGCCATAGA TTGTGTCAAC
    TATGAGGGAC AATCTGCTTA TGATGTAGCA GACATGTTGA AGCAGTATTT TCGAGACCTT
    CCTGAGCCGC TAATGACGAA CAAACTCTCA GAAACCTTTC TGCAGATCTA CCAGTGTGTG
    CCCAAGGACC AGCGCCTGCA GGCCATGAAG GCCGCCATTA TGCTCCTGCC TGATGAGAAC
    CGAGAGGTCC TACAGACACT TCTTTATTTC TTGAGCGATG TCACAGCAGC CGTAAAAGAA
    AACCAGATGA CTCCCACCAA CCTGGCCGTG TGCTTAGCAC CTTCCCTTTT CCATCTCAAC
    ACCCTGAAGA GAGAGAATTC TTCTCCAAGG GTAATGCAAA GAAAACAAAG TTTGGGCAAA
    CCAGATCAGA AAGATTTGAA TGAAAACCTT GCTGCCACTC AGGGTCTGGC CCATATGATT
    GCTGAATGCA AGAAGCTTTT CCAGGTTCCT GAGGAAATGA GTCGATGTCG GAATTCCTAC
    ACGGAACAGG AGCTGAAGCC CCTCACTCTA GAAGCATTAG GACGCCTGCG TAATGATGAG
    TCTGCTGACT ACCAGCACTT CCTCCAGGAC TGTGTGGATA GCCTGTTTAA AGAAGTCAAG
    GAGAAGTTTA AAGGCTGGGT CAGCTACTCT ACATCGGAGC AAGCTGAGCT GTCCTACAAG
    AAGGTGAGCG AAGGACCACC TCTGAGGCTT TGGCGGTCGA CCATTGAAGT CCCTGCAATG
    CCTGAGGAAA TCCTAAAGCG CCTACTTAAA GAGCAGCACC TCTGGGATGT AGACCTGTTA
    GATTCAAAAG TGATTGAAAT TCTAGACAGC CAAACTGAAA TCTACCAGTA TGTCCAAAAC
    AGTATGGCGC CCCATCCCGC TCGGGACTAC GTTGTTTTGA GAACATGGAG GACTAATTTA
    CCAAAGGGGG CATGTGCCCT TTTACTCACC TCTGTGGATC ACGACCGGGC ACCTGTGGTG
    GGGGTGAGAG TCAATGTGCT CCTCTCCAGG TATCTGATCG AACCCTGTGG ATCAGGGAAA
    TCTAAACTCA CCTACATGTG CAGAGCTGAC TTAAGGGGCC ACATGCCAGA GTGGTACACA
    AAATCTTTCG GACATCTGTG TGCAGCTGAA GTTGTGAAGA TCCGAGACTC TTTCAGCAAT
    CAGAACACCG AAACCAAAGA CACCAAATCT AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138543.1
    CDS219..3494
    Translation

    Target ORF information:

    RefSeq Version NM_001145071.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DLC1 Rho GTPase activating protein (DLC1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145071.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTGCAGGA GGAAGCCGGA CATCATGATC CTAACACAAA TTGAAGCCAA GGAAGCTTGT 
    GATTGGCTAC GGGCAACAGG TTTCCCCCAG TATGCACAGC TTTACGAAGA TCTTCTGTTC
    CCCATTGATA TTTCCTCGGT CAAGAGAGAG CATGACTTTT TGGACAGAGA TGCCATTGAG
    GCTTTATGCA GGCGTCTAAA TACTCTAAAC AAATGTGCGG TGATGAAGCT GGAAATTAGT
    CCTCATCGGA AGAGAAGTGA GGATTCGGAT GAGGACGAGC CTTGTGCAAT AAGCGGCAAG
    TGGACCTTCC AGAGAGACAG CAAGAGGTGG TCACGGCTGG AGGAGTTTGA TGTCTTTTCT
    TCAAAGCTGG ACCCAGCCCC CGGGGCGCCC GCCGAGGCCC CCCTGAAGAC CGCGGCGAGC
    CACGAAAGCA TGCTGACAGA GCTCAGCGAG CGCCAGGAGG TGGCTTCGGT CCTCAGCCTC
    AGCAGCACCG GCAGTCTCCC CGTGCACGCG CCCCACGCGG GGGACGCGGC AACGCCCCGG
    ACCAACTCGG TCATCAGCGT CTGCTCCTCC GGCCACTTCG TGGGCAACGA CGACTCCTTC
    TGCAGCCTGC CCTCTCCCAA GGAACTGTCC AGCTTCAGCT TCAGCATGAA AGGCCAAGAG
    AAGAACGCCA AGTCCAAGAC GCGCAGCCTG CTGAAGCGCA TGGAGAGCCT GAAGCTCAGG
    GGCTCGCCCC ACAGCAAGCA CAAGGCGCCT TCCAAGCTGG GGCTGATCAT CAGCGGGCCC
    ATCCTGCAGG AGGGGATGGA CGAGGAGAAG CTGAAGCAGC TCAACTGTGT GGAGATCTCC
    GCGCTCAACG GCAACCGCAT CAACGTGCCG GCCGTGCGCA AGCGGAGCGT CTCCAACTCC
    ACGCAGACCA GCAGCAGCAG CAGCCAGTCG GAGACCAGCA GCGCCGTGAG CACGCCCAGC
    CCGGTCACCA GGACTCGGAG CCTCAGTGCC TGCAACAAGC GGGTGGGCAT GTACTTAGAG
    GGCTTTGACC CGTTCAATCA GTCCACGTTC AACAACGTTA TGGAGCAGAA CTGTAAGAAC
    CGCGAGAGCT ACCCAGAGGA CACAGTGTTC TACATCCCAG AAGATCACAA GCCTGGCACT
    TTCCCCAAGG CCCTCTCCAA TGGCAGTTTC CCTCCCTCAG GGAATAACAG CTCTGTCAAC
    TGGAGGACTG GAAGCTTCCA CGGCCCCGGC CACATCAGCC TGAGGAGGGA AAACAGCAGT
    GACAGCCCCA AGGAGCTCAA GAGGCGCAAT TCCTCCAGTT CCATGAGCAG CCGCCTGAGT
    ATCTATGACA ACGTGCCAGG CTCCATCCTC TATTCCAGTT CAGGGGACCT GGCCGACCTG
    GAGAACGAGG ACATCTTCCC CGAGCTGGAT GACATCCTCT ACCATGTGAA GGGGATGCAA
    AGGATAGTCA ACCAGTGGTC CGAGAAGTTT TCAGACGAGG GGGACTCTGA CTCAGCCCTG
    GACTCGGTCT CTCCATGCCC CTCCTCTCCC AAACAGATAC ACCTGGATGT GGACAACGAC
    CGAGCCACGC CCAGTGACCT AGACAGTACG GGCAACTCAC TGAATGAACC AGAAGAGCCC
    TCAGACATCC CGGAGAGGAG GGATTCTGGG GTTGGGGCCT CCCTGACAAG GTCCAACAGG
    CACAGGCTGA GATGGCACAG TTTCCAGAGC TCCCATCGGC CGAGCTTAAA CTCTGTATCC
    CTGCAGATCA ACTGCCAGTC TGTGGCCCAG ATGAACCTGC TGCAGAAATA CTCGCTCCTA
    AAGTTAACTG CCCTGCTGGA GAAATACACA CCCTCTAATA AGCATGGTTT TAGCTGGGCT
    GTGCCCAAGT TCATGAAAAG GATCAAGGTT CCAGACTACA AGGACCGGAA TGTATTCGGG
    GTCCCTCTGA CAGTCAATGT GCAGCGCACA GGACAGCCCC TGCCCCAGAG CATTCAGCAG
    GCCATGCGCT ACCTCCGCAA CCACTGTTTG GATCAGGTTG GGCTCTTCAG AAAATCAGGC
    GTCAAATCCC GAATTCAGGC TCTGCGCCAG ATGAATGAAA GTGCCATAGA TTGTGTCAAC
    TATGAGGGAC AATCTGCTTA TGATGTAGCA GACATGTTGA AGCAGTATTT TCGAGACCTT
    CCTGAGCCGC TAATGACGAA CAAACTCTCA GAAACCTTTC TGCAGATCTA CCAGTGTGTG
    CCCAAGGACC AGCGCCTGCA GGCCATGAAG GCCGCCATTA TGCTCCTGCC TGATGAGAAC
    CGAGAGGTCC TACAGACACT TCTTTATTTC TTGAGCGATG TCACAGCAGC CGTAAAAGAA
    AACCAGATGA CTCCCACCAA CCTGGCCGTG TGCTTAGCAC CTTCCCTTTT CCATCTCAAC
    ACCCTGAAGA GAGAGAATTC TTCTCCAAGG GTAATGCAAA GAAAACAAAG TTTGGGCAAA
    CCAGATCAGA AAGATTTGAA TGAAAACCTT GCTGCCACTC AGGGTCTGGC CCATATGATT
    GCTGAATGCA AGAAGCTTTT CCAGGTTCCT GAGGAAATGA GTCGATGTCG GAATTCCTAC
    ACGGAACAGG AGCTGAAGCC CCTCACTCTA GAAGCATTAG GACGCCTGCG TAATGATGAG
    TCTGCTGACT ACCAGCACTT CCTCCAGGAC TGTGTGGATA GCCTGTTTAA AGAAGTCAAG
    GAGAAGTTTA AAGGCTGGGT CAGCTACTCT ACATCGGAGC AAGCTGAGCT GTCCTACAAG
    AAGGTGAGCG AAGGACCACC TCTGAGGCTT TGGCGGTCGA CCATTGAAGT CCCTGCAATG
    CCTGAGGAAA TCCTAAAGCG CCTACTTAAA GAGCAGCACC TCTGGGATGT AGACCTGTTA
    GATTCAAAAG TGATTGAAAT TCTAGACAGC CAAACTGAAA TCTACCAGTA TGTCCAAAAC
    AGTATGGCGC CCCATCCCGC TCGGGACTAC GTTGTTTTGA GAACATGGAG GACTAATTTA
    CCAAAGGGGG CATGTGCCCT TTTACTCACC TCTGTGGATC ACGACCGGGC ACCTGTGGTG
    GGGGTGAGAG TCAATGTGCT CCTCTCCAGG TATCTGATCG AACCCTGTGG ATCAGGGAAA
    TCTAAACTCA CCTACATGTG CAGAGCTGAC TTAAGGGGCC ACATGCCAGA GTGGTACACA
    AAATCTTTCG GACATCTGTG TGCAGCTGAA GTTGTGAAGA TCCGAGACTC TTTCAGCAAT
    CAGAACACCG AAACCAAAGA CACCAAATCT AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe155266
    Clone ID Related Accession (Same CDS sequence) NM_001313797.1
    Accession Version NM_001313797.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-23
    Organism Canis lupus familiaris(dog)
    Product rho GTPase-activating protein 7 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010443.1, FJ602870.1 and AAEX03010441.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data from different breeds because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATTGGGCAG 
    CCTTTTAATT CTGATGATCA TAACCCAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG
    CAGGCAAGTA TGGAAAAAGA TGCAACTTTA AATGTGGATC GTAAAGAGAA GTGTGTTTCA
    CTACCAGACT GCTGCCATGG TTCAGAGATG AGAGACTTTC CTGGGAGGCC AATGGGTCAC
    ATTTCAAAGG AGGTGGATGA AAATGACAGC CATGAAGATG AAGATCAGTT TCTTTCTCTG
    GAGGCCAGCA CAGAAACACT AGTACATGTT TCTGATGAGG ATGCCGATTC TGATCCATAT
    ATTACAGATG ATAAGCAGAT TTTAACTACC CAAGGACATA AAATATCAGA CCAACATAGC
    AGCAGAGGAG CAGGCTCCTT AATAAAGACA CTGCCCATCA TGGAAGGTAA CCAAAGTAAC
    TCCTGGAGAA TGGCTGGTGG AGCTGACTTA GGACTGGTAG AAGAAAGGGG AGAGAAAAAA
    GTTACTGACA TTGTAAGCAA AAGCCTGGAG CTTTGTAATG ATATAGGTTT AAGTGAAAGA
    AAAGATGCAC CTCACATAAG TGCATGTGAC TCTTTGACTG TGAAAGATAT TGTGCCTGAG
    AAACAATTGT TTAATTCTGC TGTGATTGCT CAGCAACGGA GGAAACTTGA CTTCCCTAAA
    GATGAACATG AAAGAAACAC CTGCAGTGTA GTACAGGATG AGTTCTTGGC TGCTCCTTGC
    ACGGACAGAG GACTGCCGTT ATTAAAAGCA GATTCTGGAA GCTGCCTTCT GCAACCTCCT
    TCCTGCCCTA GTGGAATGTC AGCTGAAAAT GGCCTAGAGA AGAGTGGTTT CTCAGAACAT
    CAAAACAAAA GTCTCCCAAA GGTCAACTCA GGAGATGGCA TGCAGTGTTT ACACTTAAAG
    GAAACTCTGG CCACCCAAGA ACCCACAGAT AACCCAGTCA GGCTTCGCAA AAGAAAGGAA
    ATAAGAGAAG ATCGAGATAG GGCGCGGCTA GACTCCATGG TGCTGCTAAT TATGAAACTG
    GACCAACTTG ATCAGGACAT TGAGAATGCC CTCAGCACCA GCTCTTCTCC ATCCAGCACA
    CCAACGAACC TGCGGCGGCA TGTTCCTGAT CTGGAGTCAG GATCTGAGAG TGGAGCAGAT
    ACCATTTCAA TAAATCAGAC ACAAATAAAT CTGTCTTCTA ACACCGAATC TACTGACCTA
    TCATCGTCCA CCCCAGTGGC CAGTTCTGGA ACCAAACCCA AGTCTACGAC TATTCCAGGC
    ATTTCAGACA AGGAAAAGGC TGAAATTGAA GCCAAGGAAG CTTGTGATTG GCTACGGGCA
    ACAGGTTTCC CCCAGTATGC ACAGCTTTAC GAAGATCTTC TGTTCCCCAT TGATATTTCC
    TCGGTCAAGA GAGAGCATGA CTTTTTGGAC AGAGATGCCA TTGAGGCTTT ATGCAGGCGT
    CTAAATACTC TAAACAAATG TGCGGTGATG AAGCTGGAAA TTAGTCCTCA TCGGAAGAGA
    AGTGAGGATT CGGATGAGGA CGAGCCTTGT GCAATAAGCG GCAAGTGGAC CTTCCAGAGA
    GACAGCAAGA GGTGGTCACG GCTGGAGGAG TTTGATGTCT TTTCTTCAAA GCTGGACCCA
    GCCCCCGGGG CGCCCGCCGA GGCCCCCCTG AAGACCGCGG CGAGCCACGA AAGCATGCTG
    ACAGAGCTCA GCGAGCGCCA GGAGGTGGCT TCGGTCCTCA GCCTCAGCAG CACCGGCAGT
    CTCCCCGTGC ACGCGCCCCA CGCGGGGGAC GCGGCAACGC CCCGGACCAA CTCGGTCATC
    AGCGTCTGCT CCTCCGGCCA CTTCGTGGGC AACGACGACT CCTTCTGCAG CCTGCCCTCT
    CCCAAGGAAC TGTCCAGCTT CAGCTTCAGC ATGAAAGGCC AAGAGAAGAA CGCCAAGTCC
    AAGACGCGCA GCCTGCTGAA GCGCATGGAG AGCCTGAAGC TCAGGGGCTC GCCCCACAGC
    AAGCACAAGG CGCCTTCCAA GCTGGGGCTG ATCATCAGCG GGCCCATCCT GCAGGAGGGG
    ATGGACGAGG AGAAGCTGAA GCAGCTCAAC TGTGTGGAGA TCTCCGCGCT CAACGGCAAC
    CGCATCAACG TGCCGGCCGT GCGCAAGCGG AGCGTCTCCA ACTCCACGCA GACCAGCAGC
    AGCAGCAGCC AGTCGGAGAC CAGCAGCGCC GTGAGCACGC CCAGCCCGGT CACCAGGACT
    CGGAGCCTCA GTGCCTGCAA CAAGCGGGTG GGCATGTACT TAGAGGGCTT TGACCCGTTC
    AATCAGTCCA CGTTCAACAA CGTTATGGAG CAGAACTGTA AGAACCGCGA GAGCTACCCA
    GAGGACACAG TGTTCTACAT CCCAGAAGAT CACAAGCCTG GCACTTTCCC CAAGGCCCTC
    TCCAATGGCA GTTTCCCTCC CTCAGGGAAT AACAGCTCTG TCAACTGGAG GACTGGAAGC
    TTCCACGGCC CCGGCCACAT CAGCCTGAGG AGGGAAAACA GCAGTGACAG CCCCAAGGAG
    CTCAAGAGGC GCAATTCCTC CAGTTCCATG AGCAGCCGCC TGAGTATCTA TGACAACGTG
    CCAGGCTCCA TCCTCTATTC CAGTTCAGGG GACCTGGCCG ACCTGGAGAA CGAGGACATC
    TTCCCCGAGC TGGATGACAT CCTCTACCAT GTGAAGGGGA TGCAAAGGAT AGTCAACCAG
    TGGTCCGAGA AGTTTTCAGA CGAGGGGGAC TCTGACTCAG CCCTGGACTC GGTCTCTCCA
    TGCCCCTCCT CTCCCAAACA GATACACCTG GATGTGGACA ACGACCGAGC CACGCCCAGT
    GACCTAGACA GTACGGGCAA CTCACTGAAT GAACCCGAAG AGCCCTCAGA CATCCCGGAG
    AGGAGGGATT CTGGGGTTGG GGCCTCCCTG ACAAGGTCCA ACAGGCACAG GCTGAGATGG
    CACAGTTTCC AGAGCTCCCA TCGGCCGAGC TTAAACTCTG TATCCCTGCA GATCAACTGC
    CAGTCTGTGG CCCAGATGAA CCTGCTGCAG AAATACTCGC TCCTAAAGTT AACTGCCCTG
    CTGGAGAAAT ACACACCCTC TAATAAGCAT GGTTTTAGCT GGGCTGTGCC CAAGTTCATG
    AAAAGGATCA AGGTTCCAGA CTACAAGGAC CGGAATGTAT TCGGGGTCCC TCTGACAGTC
    AATGTGCAGC GCACAGGACA GCCCCTGCCC CAGAGCATTC AGCAGGCCAT GCGCTACCTC
    CGCAACCACT GTTTGGATCA GGTTGGGCTC TTCAGAAAAT CAGGCGTCAA ATCCCGAATT
    CAGGCTCTGC GCCAGATGAA TGAAAGTGCC ATAGATTGTG TCAACTATGA GGGACAATCT
    GCTTATGATG TAGCAGACAT GTTGAAGCAG TATTTTCGAG ACCTTCCTGA GCCGCTAATG
    ACGAACAAAC TCTCAGAAAC CTTTCTGCAG ATCTACCAGT GTGTGCCCAA GGACCAGCGC
    CTGCAGGCCA TGAAGGCCGC CATTATGCTC CTGCCTGATG AGAACCGAGA GGTCCTACAG
    ACACTTCTTT ATTTCTTGAG CGATGTCACA GCAGCCGTAA AAGAAAACCA GATGACTCCC
    ACCAACCTGG CCGTGTGCTT AGCACCTTCC CTTTTCCATC TCAACACCCT GAAGAGAGAG
    AATTCTTCTC CAAGGGTAAT GCAAAGAAAA CAAAGTTTGG GCAAACCAGA TCAGAAAGAT
    TTGAATGAAA ACCTTGCTGC CACTCAGGGT CTGGCCCATA TGATTGCTGA ATGCAAGAAG
    CTTTTCCAGG TTCCTGAGGA AATGAGTCGA TGTCGGAATT CCTACACGGA ACAGGAGCTG
    AAGCCCCTCA CTCTAGAAGC ATTAGGACGC CTGCGTAATG ATGAGTCTGC TGACTACCAG
    CACTTCCTCC AGGACTGTGT GGATAGCCTG TTTAAAGAAG TCAAGGAGAA GTTTAAAGGC
    TGGGTCAGCT ACTCTACATC GGAGCAAGCT GAGCTGTCCT ACAAGAAGGT GAGCGAAGGA
    CCACCTCTGA GGCTTTGGCG GTCGACCATT GAAGTCCCTG CAATGCCTGA GGAAATCCTA
    AAGCGCCTAC TTAAAGAGCA GCACCTCTGG GATGTAGACC TGTTAGATTC AAAAGTGATT
    GAAATTCTAG ACAGCCAAAC TGAAATCTAC CAGTATGTCC AAAACAGTAT GGCGCCCCAT
    CCCGCTCGGG ACTACGTTGT TTTGAGAACA TGGAGGACTA ATTTACCAAA GGGGGCATGT
    GCCCTTTTAC TCACCTCTGT GGATCACGAC CGGGCACCTG TGGTGGGGGT GAGAGTCAAT
    GTGCTCCTCT CCAGGTATCT GATCGAACCC TGTGGATCAG GGAAATCTAA ACTCACCTAC
    ATGTGCAGAG CTGACTTAAG GGGCCACATG CCAGAGTGGT ACACAAAATC TTTCGGACAT
    CTGTGTGCAG CTGAAGTTGT GAAGATCCGA GACTCTTTCA GCAATCAGAA CACCGAAACC
    AAAGACACCA AATCTAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300726.1
    CDS442..5022
    Translation

    Target ORF information:

    RefSeq Version NM_001313797.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DLC1 Rho GTPase activating protein (DLC1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313797.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGTAG CTATCAGAAA GAGAAGCTGG GAAGAACATG TGACCCAATG GATTGGGCAG 
    CCTTTTAATT CTGATGATCA TAACCCAGCA TGTCATCATG GACTAGTAGC TGACAGCTTG
    CAGGCAAGTA TGGAAAAAGA TGCAACTTTA AATGTGGATC GTAAAGAGAA GTGTGTTTCA
    CTACCAGACT GCTGCCATGG TTCAGAGATG AGAGACTTTC CTGGGAGGCC AATGGGTCAC
    ATTTCAAAGG AGGTGGATGA AAATGACAGC CATGAAGATG AAGATCAGTT TCTTTCTCTG
    GAGGCCAGCA CAGAAACACT AGTACATGTT TCTGATGAGG ATGCCGATTC TGATCCATAT
    ATTACAGATG ATAAGCAGAT TTTAACTACC CAAGGACATA AAATATCAGA CCAACATAGC
    AGCAGAGGAG CAGGCTCCTT AATAAAGACA CTGCCCATCA TGGAAGGTAA CCAAAGTAAC
    TCCTGGAGAA TGGCTGGTGG AGCTGACTTA GGACTGGTAG AAGAAAGGGG AGAGAAAAAA
    GTTACTGACA TTGTAAGCAA AAGCCTGGAG CTTTGTAATG ATATAGGTTT AAGTGAAAGA
    AAAGATGCAC CTCACATAAG TGCATGTGAC TCTTTGACTG TGAAAGATAT TGTGCCTGAG
    AAACAATTGT TTAATTCTGC TGTGATTGCT CAGCAACGGA GGAAACTTGA CTTCCCTAAA
    GATGAACATG AAAGAAACAC CTGCAGTGTA GTACAGGATG AGTTCTTGGC TGCTCCTTGC
    ACGGACAGAG GACTGCCGTT ATTAAAAGCA GATTCTGGAA GCTGCCTTCT GCAACCTCCT
    TCCTGCCCTA GTGGAATGTC AGCTGAAAAT GGCCTAGAGA AGAGTGGTTT CTCAGAACAT
    CAAAACAAAA GTCTCCCAAA GGTCAACTCA GGAGATGGCA TGCAGTGTTT ACACTTAAAG
    GAAACTCTGG CCACCCAAGA ACCCACAGAT AACCCAGTCA GGCTTCGCAA AAGAAAGGAA
    ATAAGAGAAG ATCGAGATAG GGCGCGGCTA GACTCCATGG TGCTGCTAAT TATGAAACTG
    GACCAACTTG ATCAGGACAT TGAGAATGCC CTCAGCACCA GCTCTTCTCC ATCCAGCACA
    CCAACGAACC TGCGGCGGCA TGTTCCTGAT CTGGAGTCAG GATCTGAGAG TGGAGCAGAT
    ACCATTTCAA TAAATCAGAC ACAAATAAAT CTGTCTTCTA ACACCGAATC TACTGACCTA
    TCATCGTCCA CCCCAGTGGC CAGTTCTGGA ACCAAACCCA AGTCTACGAC TATTCCAGGC
    ATTTCAGACA AGGAAAAGGC TGAAATTGAA GCCAAGGAAG CTTGTGATTG GCTACGGGCA
    ACAGGTTTCC CCCAGTATGC ACAGCTTTAC GAAGATCTTC TGTTCCCCAT TGATATTTCC
    TCGGTCAAGA GAGAGCATGA CTTTTTGGAC AGAGATGCCA TTGAGGCTTT ATGCAGGCGT
    CTAAATACTC TAAACAAATG TGCGGTGATG AAGCTGGAAA TTAGTCCTCA TCGGAAGAGA
    AGTGAGGATT CGGATGAGGA CGAGCCTTGT GCAATAAGCG GCAAGTGGAC CTTCCAGAGA
    GACAGCAAGA GGTGGTCACG GCTGGAGGAG TTTGATGTCT TTTCTTCAAA GCTGGACCCA
    GCCCCCGGGG CGCCCGCCGA GGCCCCCCTG AAGACCGCGG CGAGCCACGA AAGCATGCTG
    ACAGAGCTCA GCGAGCGCCA GGAGGTGGCT TCGGTCCTCA GCCTCAGCAG CACCGGCAGT
    CTCCCCGTGC ACGCGCCCCA CGCGGGGGAC GCGGCAACGC CCCGGACCAA CTCGGTCATC
    AGCGTCTGCT CCTCCGGCCA CTTCGTGGGC AACGACGACT CCTTCTGCAG CCTGCCCTCT
    CCCAAGGAAC TGTCCAGCTT CAGCTTCAGC ATGAAAGGCC AAGAGAAGAA CGCCAAGTCC
    AAGACGCGCA GCCTGCTGAA GCGCATGGAG AGCCTGAAGC TCAGGGGCTC GCCCCACAGC
    AAGCACAAGG CGCCTTCCAA GCTGGGGCTG ATCATCAGCG GGCCCATCCT GCAGGAGGGG
    ATGGACGAGG AGAAGCTGAA GCAGCTCAAC TGTGTGGAGA TCTCCGCGCT CAACGGCAAC
    CGCATCAACG TGCCGGCCGT GCGCAAGCGG AGCGTCTCCA ACTCCACGCA GACCAGCAGC
    AGCAGCAGCC AGTCGGAGAC CAGCAGCGCC GTGAGCACGC CCAGCCCGGT CACCAGGACT
    CGGAGCCTCA GTGCCTGCAA CAAGCGGGTG GGCATGTACT TAGAGGGCTT TGACCCGTTC
    AATCAGTCCA CGTTCAACAA CGTTATGGAG CAGAACTGTA AGAACCGCGA GAGCTACCCA
    GAGGACACAG TGTTCTACAT CCCAGAAGAT CACAAGCCTG GCACTTTCCC CAAGGCCCTC
    TCCAATGGCA GTTTCCCTCC CTCAGGGAAT AACAGCTCTG TCAACTGGAG GACTGGAAGC
    TTCCACGGCC CCGGCCACAT CAGCCTGAGG AGGGAAAACA GCAGTGACAG CCCCAAGGAG
    CTCAAGAGGC GCAATTCCTC CAGTTCCATG AGCAGCCGCC TGAGTATCTA TGACAACGTG
    CCAGGCTCCA TCCTCTATTC CAGTTCAGGG GACCTGGCCG ACCTGGAGAA CGAGGACATC
    TTCCCCGAGC TGGATGACAT CCTCTACCAT GTGAAGGGGA TGCAAAGGAT AGTCAACCAG
    TGGTCCGAGA AGTTTTCAGA CGAGGGGGAC TCTGACTCAG CCCTGGACTC GGTCTCTCCA
    TGCCCCTCCT CTCCCAAACA GATACACCTG GATGTGGACA ACGACCGAGC CACGCCCAGT
    GACCTAGACA GTACGGGCAA CTCACTGAAT GAACCCGAAG AGCCCTCAGA CATCCCGGAG
    AGGAGGGATT CTGGGGTTGG GGCCTCCCTG ACAAGGTCCA ACAGGCACAG GCTGAGATGG
    CACAGTTTCC AGAGCTCCCA TCGGCCGAGC TTAAACTCTG TATCCCTGCA GATCAACTGC
    CAGTCTGTGG CCCAGATGAA CCTGCTGCAG AAATACTCGC TCCTAAAGTT AACTGCCCTG
    CTGGAGAAAT ACACACCCTC TAATAAGCAT GGTTTTAGCT GGGCTGTGCC CAAGTTCATG
    AAAAGGATCA AGGTTCCAGA CTACAAGGAC CGGAATGTAT TCGGGGTCCC TCTGACAGTC
    AATGTGCAGC GCACAGGACA GCCCCTGCCC CAGAGCATTC AGCAGGCCAT GCGCTACCTC
    CGCAACCACT GTTTGGATCA GGTTGGGCTC TTCAGAAAAT CAGGCGTCAA ATCCCGAATT
    CAGGCTCTGC GCCAGATGAA TGAAAGTGCC ATAGATTGTG TCAACTATGA GGGACAATCT
    GCTTATGATG TAGCAGACAT GTTGAAGCAG TATTTTCGAG ACCTTCCTGA GCCGCTAATG
    ACGAACAAAC TCTCAGAAAC CTTTCTGCAG ATCTACCAGT GTGTGCCCAA GGACCAGCGC
    CTGCAGGCCA TGAAGGCCGC CATTATGCTC CTGCCTGATG AGAACCGAGA GGTCCTACAG
    ACACTTCTTT ATTTCTTGAG CGATGTCACA GCAGCCGTAA AAGAAAACCA GATGACTCCC
    ACCAACCTGG CCGTGTGCTT AGCACCTTCC CTTTTCCATC TCAACACCCT GAAGAGAGAG
    AATTCTTCTC CAAGGGTAAT GCAAAGAAAA CAAAGTTTGG GCAAACCAGA TCAGAAAGAT
    TTGAATGAAA ACCTTGCTGC CACTCAGGGT CTGGCCCATA TGATTGCTGA ATGCAAGAAG
    CTTTTCCAGG TTCCTGAGGA AATGAGTCGA TGTCGGAATT CCTACACGGA ACAGGAGCTG
    AAGCCCCTCA CTCTAGAAGC ATTAGGACGC CTGCGTAATG ATGAGTCTGC TGACTACCAG
    CACTTCCTCC AGGACTGTGT GGATAGCCTG TTTAAAGAAG TCAAGGAGAA GTTTAAAGGC
    TGGGTCAGCT ACTCTACATC GGAGCAAGCT GAGCTGTCCT ACAAGAAGGT GAGCGAAGGA
    CCACCTCTGA GGCTTTGGCG GTCGACCATT GAAGTCCCTG CAATGCCTGA GGAAATCCTA
    AAGCGCCTAC TTAAAGAGCA GCACCTCTGG GATGTAGACC TGTTAGATTC AAAAGTGATT
    GAAATTCTAG ACAGCCAAAC TGAAATCTAC CAGTATGTCC AAAACAGTAT GGCGCCCCAT
    CCCGCTCGGG ACTACGTTGT TTTGAGAACA TGGAGGACTA ATTTACCAAA GGGGGCATGT
    GCCCTTTTAC TCACCTCTGT GGATCACGAC CGGGCACCTG TGGTGGGGGT GAGAGTCAAT
    GTGCTCCTCT CCAGGTATCT GATCGAACCC TGTGGATCAG GGAAATCTAA ACTCACCTAC
    ATGTGCAGAG CTGACTTAAG GGGCCACATG CCAGAGTGGT ACACAAAATC TTTCGGACAT
    CTGTGTGCAG CTGAAGTTGT GAAGATCCGA GACTCTTTCA GCAATCAGAA CACCGAAACC
    AAAGACACCA AATCTAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.