MGAM cDNA ORF clone, Canis lupus familiaris(dog)

The following MGAM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGAM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe293843 XM_022403727.1
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe17967 XM_014119954.1
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe17967 XM_022403728.1
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe17967 XM_014119955.1
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe17967 XM_022403729.1
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe17967 XM_005629542.2
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe17967 XM_014119957.1
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe17967 XM_014119956.2
Latest version!
Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe17967 XM_014119956.1 Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe293843
    Clone ID Related Accession (Same CDS sequence) XM_022403727.1
    Accession Version XM_022403727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product maltase-glucoamylase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGATGGA GAGCAAGACT GCAAGGTTGT TCTCAAACAA TGGCAAGAAG GAGGTGGAAA 
    GATTTCACGA CTTTGGAGCT TATGCTCAGT GTTCTTCTGC TTATGGTGTT TATCATCACT
    ATTCCTCTGT TTGTGCTTTC AGCTCAAGAT TCTTTGGAAT CAAAAGATCC TGGGACAGTA
    GTTACCCCAG ATCCTGGGAC AGCAAGTACC ACCCCTAATT CTGCTGTGTG CCCGGTGGTA
    GATGAAGCAG AACGGATCAA CTGCATTCCT GACCAGCCAC CAACAAAGGC CACATGTGAT
    CAGCGTGGGT GCTGCTGGAG ACCTCAGGGG ACCATAAATG TACCCTGGTG CTACTATTCT
    AGGAGTCATG GTTATCAAGG GGAGGGTGAT GTTGTAAAAA CGAATGCAGG GTTCACAGCC
    CAGTTGAAGA GGTTGCCTTC TCCATCCCTA TTTGGAAATG ATGTCAACAA TGTCCTTCTC
    ACAGCAGAGT ATCAGACGTC CAATCGCTTC CACTTTAAGT TAACTGATCA GAGCAAAGAC
    AGGTATGAAG TGCCCCATGA ACATGTGCAA CCCTTTAAAG GAGATGCTGC TTCACCCTTG
    ACCTATGAAG TTGAGGTTTC CAAACAGCCA TTTAGCATCA AAGTAATCAG AACAAGCAAT
    AATCGTGTTC TGCTGGACTC AGGCATTGGA CCCCTCCTGT TTGCTGATCA GTTCCTGCAA
    TTCTCCACCC GACTGCCCAG TGCTAATGTG TACGGTCTGG GAGAACAGGT GCACCAACAG
    TACAGGCATG ATATGAATTG GAAGACCTGG CCTATATTTG CCCGGGACAC AACTCCCAAT
    GGGGATGGAA CTAACTTGTA TGGTGCACAG ACATTCTTCC TGTGTCTTGA AGATGCTAGT
    GGATTGTCCT TTGGAGTGTT TCTAATGAAC AGCAATGCCA TGGAGGTTGC CCTCCAGCCC
    ACCCCAGCCA TCACTTACCG CACCATCGGG GGCATTCTCG ACTTCTATGT GTTTTTGGGA
    AACACTCCAG AGCAAGTTGT TCAAGAGTAT CTAGAGCTCA TTGGACGGCC AGCCCTTCCC
    TCATACTGGG CCCTTGGATT TCATCTCAGT CGTTATGATT ATGGAACGCT AGAGAGCATG
    AGGGAAGTCG TGCAGAGAAA CCGTGTGGCT CGGCTCCCTT ATGACGTGCA GCATGCTGAT
    ATTGATTATA TGCATGAGAG GAAGGACTTC ACTTATGATC CAGTAAATTA TAAAGGCTTC
    CCTGAGTTTG TTAAGGAGTT ACACAATAAC AGTCAGAAAT TGGTCATCAT TGTGGATCCA
    GCCATCTCCA ACAACTCTTC CCCCAGCAAT CCTTACGGCC CATATGACAG AGGTTCAGGT
    GCAAAGATAT GGGTGAATGC TTCAGATGGG TTGACGCCGC TCATTGGAGA GGTCTGGCCT
    GGAAAAACTG TGTTTCCTGA TTATACCAAC CCTAGCTGTG CTGTTTGGTG GGCAAAGGAA
    TTTGAGCTTT TCCACAACAA AATAGAATTT GATGGAATCT GGATTGATAT GAATGAAGTC
    TCCAACTTTG TTGATGGTTC CATTTCAGGG TGTTCCACGA GCCATCTTAA TTATCCCCCA
    TTTGTTCCCA GAATCCTGGA TGGGTACCTG TTCTCCAAGA GTCTCTGCAT GGATGCAGTG
    CAGCATTGGG GCAAGCAGTA CGACGTCCAC AATCTGTATG GCTACTCCAT GGCGATCGCC
    ACGGCAGAAG CTGTCAAGAC CGTGTTCCCT AATAAGAGAA GCTTCATTTT AACCCGTTCT
    ACCTTTGCGG GATCTGGCAA GTTTGCAGCC CACTGGTTAG GAGACAATGC GGCCACCTGG
    AATGATCTTC GGTGGTCTAT CCCTGGCATG CTCGAGTTCA ACCTTTTTGG TATCCCAATG
    GTGGGTGCTG ACATATGTGG CTTTGCACTG GACACCTCTG AGGAGCTCTG TAGGAGGTGG
    ATGCAGCTGG GAGCATTTTA TCCATTTTCC AGGAATCACA ATGGCCAAGG CTACAAGGCC
    CAGGATCCTG CCTCTTTTGG AGCTGACTCT CTGCTGTTGA ACTCCTCCAG GCACTACCTT
    AACATCCGTT ACACTCTATT ACCCTACCTC TACACCCTCT TCTACCGTGC CCACAGCCGG
    GGGGACACAG TGGCCAGGCC CCTTATGCAT GAGTTCTATG GGGATAGCAG TACATGGGAT
    GTACACCAAC AGTTTTTATG GGGACCCGGC CTCCTCATCA CTCCAGTTCT GGATGAGGGT
    GCAGAGAAAG TGATGGCTTA TATACCCGAT GCCGTCTGGT ACGATTATGA GACTGGGGCC
    CGAGCAAGAT GGAGGAAGCA AAGAGTGGAG ATGGGACTTC CTGCAGACAA AATTGGACTT
    CACCTTCGCG GAGGCCACAT CTTTCCCACA CAACAGCCAG CCACCACCAC GGTGGCCAGT
    CGCCGGAACC CCCTTGGTCT TATCATTGCC CTGGATGAAA ACAAAGAAGC AAAAGGCGAA
    CTTTTCTGGG ATGATGGGGA AACTAAAGAT ACTGTGGCCA ATCAAGTTTA TCTCTTATGT
    GAGTTTTCTG TCACCCAAAA CCGCTTGGAT GTGAAGATTT TGCAGTCAAC TTACAAAGAC
    CCCAATAATT TAGTATTTAA AGAGATTAAA ATTCTTGGGA CCCAGGAACC TACCAATGTT
    ATAGTGAAAC ACAATGGTAT CCCAAGTCAA ACTTCTCCTA ATGTCACTTA TGATTCTAAC
    CTACAGGTTG CTCTTATCAC AGAAATTGAT CTTATCCTCG GAGAAGCATA CACGGTGGAG
    TGGGACCTGA AGATAAGGGA TGAAGAGAAA ATAGACTGTT TTCCTGATGA GAGCGGTGCT
    TCTGCAGCAA ACTGTACTGC CCGTGGCTGT GCCTGGGAGG CATCCAGTTC TCCTGGAGTC
    CCATTCTGTT ATTTTGTCAA CGATCTGTAC TCTGTCAGCG ATGTTCAGTA TGACTCACAT
    GGAGCCACTG CTACCATCTC CTTAAAATCT TCTGTCTATG CCTCCGCTCT GCCCTCAGTG
    CCCGTGACTT CTCTCCGCCT ACGTGTGACC TACCATAAGA ATGACATGCT ACAGTTTAAG
    ATTTATGATC CCAATAATAA TCGGTATGAA GTCCCAGTCC CTCTCAACAT ACCCAGGGTT
    CCATCCAGCA CCTCTGAGAG TCAACTCTAT GATGTCCTCA TTAAGAAGAA TCCATTTGGA
    ATCGAAATTC GCCGGAAGAG TACGGGCACT GTGATTTGGG ACTCTCAGCT CCTTGGCTTT
    ACCTTCAATG ACATGTTCAT CCGTATCTCC ACTCGCCTTC CCTCCCAGTA CCTCTATGGT
    TTTGGGGAAA CTGAGCACAC AGCCTTTCGG AGAGACTTGA ACTGGCACAC ATGGGGGATG
    TTCTCCAGGG ACCAACCTCC GGGGTACAAG AAGAATTCCT ATGGTGTCCA CCCCTACTAC
    ATGGCACTGG AGGAGGATGG TAGTGCCCAT GGGGTGTTAC TGCTCAACAG CAATGCCATG
    GATGTGACAT TCCAGCCCCT GCCTGCCTTG ACATACCGCA CCATAGGAGG AATTCTGGAC
    TTTTATGTGT TCTTGGGGCC AACTCCAGAG CTTGTCACTC AGCAGTATAC TGAGATGATT
    GGTCGGCCAG TGATGGTTCC TTACTGGTCC TTGGGGTTTC AGCTGTGTCG CTATGGCTAC
    CAGAACGACT CTGAGATTGC CAGCCTGTAT GATGAGATGG TGGCTGCCCA GATCCCTTAT
    GATGTGCAGT ACTCCGACAT CGACTACATG GAGCGGCAGC TGGACTTCAC CCTCAGCCCC
    AAGTTCGCAG GATTTCCAGC TCTGATTACT CGCATGAAGG CCAACGGAAT GCGGGTCATC
    CTCATTCTGG ACCCAGCCAT TTCTGGTAAT GAGACGCAGC CCTATCCTGC ATTCACTCGG
    GGTGTGGAGG ATGATGTCTT CATCAAAGCT CCAAATGGTG GAGGCATTGT CTGGGGGAAG
    GTGTGGCCTG ATTTCCCTGA TGTCGTCATA AACGCATCCC TAGACTGGGA TAGTCAAGTG
    GAGCTGTACC GAGCTTATGT GGCCTTTCCT GACTTTTTCC GTAACTCCAC TACCACATGG
    TGGAAGAGGG AGCTGCAAGA ACTATACACC AACCCCCAGA ATCCAGAGAG GAGCTTGAAG
    TTTGACGGCA TGTGGATTGA TATGAATGAG CCAGCAAGTT TTGTGAATGG AGCAGTCCCC
    CCAGGCTGCA GGGATGCCAC TATGAACCAC CCCCCCTATA TGCCACATTT GGAGTCCAGG
    GATAAAGGCC TGAGCAGCAA GACCCTGTGC ATGGAGAGCG AGCAGGTCCT GCCAGATGGC
    TCACGGGTGC GGCACTATGA TGTTCACAGC CTGTATGGAT GGGCCCAGAC CAGACCCACC
    TACGAAGCTG TGCAGGAGGT GACGGGACAG AGAGGGATTG TCATCACCCG CTCCACATTC
    CCCTCTTCTG GCCGCTGGGG AGGCCACTGG CTGGGTGACA ACACGGCCGC GTGGGACCAG
    CTGAAGAAGT CTATCATTGG CATGATGGAG TTCAGCCTCT TCGGTATATC TTATACAGGA
    GCAGATATTT GTGGGTTCTT TCAAGATGCT GAATATGAGA TGTGTGTTCG CTGGATGCAG
    CTGGGAGCCT TTTACCCCTT TTCGAGGAAC CACAACACCA TTGGGACCAG GAGGCAAGAC
    CCTGTGTCCT GGAATGCAAC CTTTGAGGAT ATCTCCAGAA GTGTGCTGCA GACCAGATAC
    ACCCTGTTGC CATATCTCTA CACCCTGATG CACCTGGCCC ACACGGAGGG CAGTACTGTC
    GTGCGGCCTC TTCTCCATGA GTTTGTGTCA GACCGGGTGA CCTGGAATGT AGACAGTCAG
    TTTCTGCTGG GCCCAGCCTT CCTAGTCAGC CCTGTCCTGG AGCCTAATGC CAGAAATGTC
    ACTGCATATT TCCCTAGAGC CCGCTGGTAT GATTACTACA CGGGTGTGGA CATTGGAGCA
    AGGGCAGAGT GGAAGTCCTT GCCAGCCCCT CTTGACCACA TTAACCTTCA TGTCCGTGGG
    GGCTACATCC TGCCCTGGCA AGAGCCTGCG CAGAACACCC ACTTAAGCCG TCAGAAATTC
    ATAGGCTTCA AGGTTGCTTT GGATGATGAG GGGGCTGCTA AAGGCTGGCT CTTCTGGGAT
    GATGGGCAAA GCATTGACAC CTATGGAAAA GGACACTATT ACCTGGCCAA CTTTTCTGCC
    AGCCAGAATA TGATGCAGAG TCATATAGTT TTCAACAAAT ACATCACCGA TGCAAACCCT
    TTGAAACTGG GCTACGTTGA GATCTGGGGA GTGGGCAGTG TTTCCATTTC CAGTGTCAGC
    ATCTCTGTGA GCGGCATGGT GATAACGCCC ACCTTCACTT ACAACCCTGC AACACAGGTG
    TTAAGCATTG ATGTGACCGG CAGAGATATC AGCCTCCATA ATTTCATTTC ACTGACGTGG
    AGAAGCACTC TGAATTTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259435.1
    CDS102..5642
    Translation

    Target ORF information:

    RefSeq Version XM_022403727.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGATGGA GAGCAAGACT GCAAGGTTGT TCTCAAACAA TGGCAAGAAG GAGGTGGAAA 
    GATTTCACGA CTTTGGAGCT TATGCTCAGT GTTCTTCTGC TTATGGTGTT TATCATCACT
    ATTCCTCTGT TTGTGCTTTC AGCTCAAGAT TCTTTGGAAT CAAAAGATCC TGGGACAGTA
    GTTACCCCAG ATCCTGGGAC AGCAAGTACC ACCCCTAATT CTGCTGTGTG CCCGGTGGTA
    GATGAAGCAG AACGGATCAA CTGCATTCCT GACCAGCCAC CAACAAAGGC CACATGTGAT
    CAGCGTGGGT GCTGCTGGAG ACCTCAGGGG ACCATAAATG TACCCTGGTG CTACTATTCT
    AGGAGTCATG GTTATCAAGG GGAGGGTGAT GTTGTAAAAA CGAATGCAGG GTTCACAGCC
    CAGTTGAAGA GGTTGCCTTC TCCATCCCTA TTTGGAAATG ATGTCAACAA TGTCCTTCTC
    ACAGCAGAGT ATCAGACGTC CAATCGCTTC CACTTTAAGT TAACTGATCA GAGCAAAGAC
    AGGTATGAAG TGCCCCATGA ACATGTGCAA CCCTTTAAAG GAGATGCTGC TTCACCCTTG
    ACCTATGAAG TTGAGGTTTC CAAACAGCCA TTTAGCATCA AAGTAATCAG AACAAGCAAT
    AATCGTGTTC TGCTGGACTC AGGCATTGGA CCCCTCCTGT TTGCTGATCA GTTCCTGCAA
    TTCTCCACCC GACTGCCCAG TGCTAATGTG TACGGTCTGG GAGAACAGGT GCACCAACAG
    TACAGGCATG ATATGAATTG GAAGACCTGG CCTATATTTG CCCGGGACAC AACTCCCAAT
    GGGGATGGAA CTAACTTGTA TGGTGCACAG ACATTCTTCC TGTGTCTTGA AGATGCTAGT
    GGATTGTCCT TTGGAGTGTT TCTAATGAAC AGCAATGCCA TGGAGGTTGC CCTCCAGCCC
    ACCCCAGCCA TCACTTACCG CACCATCGGG GGCATTCTCG ACTTCTATGT GTTTTTGGGA
    AACACTCCAG AGCAAGTTGT TCAAGAGTAT CTAGAGCTCA TTGGACGGCC AGCCCTTCCC
    TCATACTGGG CCCTTGGATT TCATCTCAGT CGTTATGATT ATGGAACGCT AGAGAGCATG
    AGGGAAGTCG TGCAGAGAAA CCGTGTGGCT CGGCTCCCTT ATGACGTGCA GCATGCTGAT
    ATTGATTATA TGCATGAGAG GAAGGACTTC ACTTATGATC CAGTAAATTA TAAAGGCTTC
    CCTGAGTTTG TTAAGGAGTT ACACAATAAC AGTCAGAAAT TGGTCATCAT TGTGGATCCA
    GCCATCTCCA ACAACTCTTC CCCCAGCAAT CCTTACGGCC CATATGACAG AGGTTCAGGT
    GCAAAGATAT GGGTGAATGC TTCAGATGGG TTGACGCCGC TCATTGGAGA GGTCTGGCCT
    GGAAAAACTG TGTTTCCTGA TTATACCAAC CCTAGCTGTG CTGTTTGGTG GGCAAAGGAA
    TTTGAGCTTT TCCACAACAA AATAGAATTT GATGGAATCT GGATTGATAT GAATGAAGTC
    TCCAACTTTG TTGATGGTTC CATTTCAGGG TGTTCCACGA GCCATCTTAA TTATCCCCCA
    TTTGTTCCCA GAATCCTGGA TGGGTACCTG TTCTCCAAGA GTCTCTGCAT GGATGCAGTG
    CAGCATTGGG GCAAGCAGTA CGACGTCCAC AATCTGTATG GCTACTCCAT GGCGATCGCC
    ACGGCAGAAG CTGTCAAGAC CGTGTTCCCT AATAAGAGAA GCTTCATTTT AACCCGTTCT
    ACCTTTGCGG GATCTGGCAA GTTTGCAGCC CACTGGTTAG GAGACAATGC GGCCACCTGG
    AATGATCTTC GGTGGTCTAT CCCTGGCATG CTCGAGTTCA ACCTTTTTGG TATCCCAATG
    GTGGGTGCTG ACATATGTGG CTTTGCACTG GACACCTCTG AGGAGCTCTG TAGGAGGTGG
    ATGCAGCTGG GAGCATTTTA TCCATTTTCC AGGAATCACA ATGGCCAAGG CTACAAGGCC
    CAGGATCCTG CCTCTTTTGG AGCTGACTCT CTGCTGTTGA ACTCCTCCAG GCACTACCTT
    AACATCCGTT ACACTCTATT ACCCTACCTC TACACCCTCT TCTACCGTGC CCACAGCCGG
    GGGGACACAG TGGCCAGGCC CCTTATGCAT GAGTTCTATG GGGATAGCAG TACATGGGAT
    GTACACCAAC AGTTTTTATG GGGACCCGGC CTCCTCATCA CTCCAGTTCT GGATGAGGGT
    GCAGAGAAAG TGATGGCTTA TATACCCGAT GCCGTCTGGT ACGATTATGA GACTGGGGCC
    CGAGCAAGAT GGAGGAAGCA AAGAGTGGAG ATGGGACTTC CTGCAGACAA AATTGGACTT
    CACCTTCGCG GAGGCCACAT CTTTCCCACA CAACAGCCAG CCACCACCAC GGTGGCCAGT
    CGCCGGAACC CCCTTGGTCT TATCATTGCC CTGGATGAAA ACAAAGAAGC AAAAGGCGAA
    CTTTTCTGGG ATGATGGGGA AACTAAAGAT ACTGTGGCCA ATCAAGTTTA TCTCTTATGT
    GAGTTTTCTG TCACCCAAAA CCGCTTGGAT GTGAAGATTT TGCAGTCAAC TTACAAAGAC
    CCCAATAATT TAGTATTTAA AGAGATTAAA ATTCTTGGGA CCCAGGAACC TACCAATGTT
    ATAGTGAAAC ACAATGGTAT CCCAAGTCAA ACTTCTCCTA ATGTCACTTA TGATTCTAAC
    CTACAGGTTG CTCTTATCAC AGAAATTGAT CTTATCCTCG GAGAAGCATA CACGGTGGAG
    TGGGACCTGA AGATAAGGGA TGAAGAGAAA ATAGACTGTT TTCCTGATGA GAGCGGTGCT
    TCTGCAGCAA ACTGTACTGC CCGTGGCTGT GCCTGGGAGG CATCCAGTTC TCCTGGAGTC
    CCATTCTGTT ATTTTGTCAA CGATCTGTAC TCTGTCAGCG ATGTTCAGTA TGACTCACAT
    GGAGCCACTG CTACCATCTC CTTAAAATCT TCTGTCTATG CCTCCGCTCT GCCCTCAGTG
    CCCGTGACTT CTCTCCGCCT ACGTGTGACC TACCATAAGA ATGACATGCT ACAGTTTAAG
    ATTTATGATC CCAATAATAA TCGGTATGAA GTCCCAGTCC CTCTCAACAT ACCCAGGGTT
    CCATCCAGCA CCTCTGAGAG TCAACTCTAT GATGTCCTCA TTAAGAAGAA TCCATTTGGA
    ATCGAAATTC GCCGGAAGAG TACGGGCACT GTGATTTGGG ACTCTCAGCT CCTTGGCTTT
    ACCTTCAATG ACATGTTCAT CCGTATCTCC ACTCGCCTTC CCTCCCAGTA CCTCTATGGT
    TTTGGGGAAA CTGAGCACAC AGCCTTTCGG AGAGACTTGA ACTGGCACAC ATGGGGGATG
    TTCTCCAGGG ACCAACCTCC GGGGTACAAG AAGAATTCCT ATGGTGTCCA CCCCTACTAC
    ATGGCACTGG AGGAGGATGG TAGTGCCCAT GGGGTGTTAC TGCTCAACAG CAATGCCATG
    GATGTGACAT TCCAGCCCCT GCCTGCCTTG ACATACCGCA CCATAGGAGG AATTCTGGAC
    TTTTATGTGT TCTTGGGGCC AACTCCAGAG CTTGTCACTC AGCAGTATAC TGAGATGATT
    GGTCGGCCAG TGATGGTTCC TTACTGGTCC TTGGGGTTTC AGCTGTGTCG CTATGGCTAC
    CAGAACGACT CTGAGATTGC CAGCCTGTAT GATGAGATGG TGGCTGCCCA GATCCCTTAT
    GATGTGCAGT ACTCCGACAT CGACTACATG GAGCGGCAGC TGGACTTCAC CCTCAGCCCC
    AAGTTCGCAG GATTTCCAGC TCTGATTACT CGCATGAAGG CCAACGGAAT GCGGGTCATC
    CTCATTCTGG ACCCAGCCAT TTCTGGTAAT GAGACGCAGC CCTATCCTGC ATTCACTCGG
    GGTGTGGAGG ATGATGTCTT CATCAAAGCT CCAAATGGTG GAGGCATTGT CTGGGGGAAG
    GTGTGGCCTG ATTTCCCTGA TGTCGTCATA AACGCATCCC TAGACTGGGA TAGTCAAGTG
    GAGCTGTACC GAGCTTATGT GGCCTTTCCT GACTTTTTCC GTAACTCCAC TACCACATGG
    TGGAAGAGGG AGCTGCAAGA ACTATACACC AACCCCCAGA ATCCAGAGAG GAGCTTGAAG
    TTTGACGGCA TGTGGATTGA TATGAATGAG CCAGCAAGTT TTGTGAATGG AGCAGTCCCC
    CCAGGCTGCA GGGATGCCAC TATGAACCAC CCCCCCTATA TGCCACATTT GGAGTCCAGG
    GATAAAGGCC TGAGCAGCAA GACCCTGTGC ATGGAGAGCG AGCAGGTCCT GCCAGATGGC
    TCACGGGTGC GGCACTATGA TGTTCACAGC CTGTATGGAT GGGCCCAGAC CAGACCCACC
    TACGAAGCTG TGCAGGAGGT GACGGGACAG AGAGGGATTG TCATCACCCG CTCCACATTC
    CCCTCTTCTG GCCGCTGGGG AGGCCACTGG CTGGGTGACA ACACGGCCGC GTGGGACCAG
    CTGAAGAAGT CTATCATTGG CATGATGGAG TTCAGCCTCT TCGGTATATC TTATACAGGA
    GCAGATATTT GTGGGTTCTT TCAAGATGCT GAATATGAGA TGTGTGTTCG CTGGATGCAG
    CTGGGAGCCT TTTACCCCTT TTCGAGGAAC CACAACACCA TTGGGACCAG GAGGCAAGAC
    CCTGTGTCCT GGAATGCAAC CTTTGAGGAT ATCTCCAGAA GTGTGCTGCA GACCAGATAC
    ACCCTGTTGC CATATCTCTA CACCCTGATG CACCTGGCCC ACACGGAGGG CAGTACTGTC
    GTGCGGCCTC TTCTCCATGA GTTTGTGTCA GACCGGGTGA CCTGGAATGT AGACAGTCAG
    TTTCTGCTGG GCCCAGCCTT CCTAGTCAGC CCTGTCCTGG AGCCTAATGC CAGAAATGTC
    ACTGCATATT TCCCTAGAGC CCGCTGGTAT GATTACTACA CGGGTGTGGA CATTGGAGCA
    AGGGCAGAGT GGAAGTCCTT GCCAGCCCCT CTTGACCACA TTAACCTTCA TGTCCGTGGG
    GGCTACATCC TGCCCTGGCA AGAGCCTGCG CAGAACACCC ACTTAAGCCG TCAGAAATTC
    ATAGGCTTCA AGGTTGCTTT GGATGATGAG GGGGCTGCTA AAGGCTGGCT CTTCTGGGAT
    GATGGGCAAA GCATTGACAC CTATGGAAAA GGACACTATT ACCTGGCCAA CTTTTCTGCC
    AGCCAGAATA TGATGCAGAG TCATATAGTT TTCAACAAAT ACATCACCGA TGCAAACCCT
    TTGAAACTGG GCTACGTTGA GATCTGGGGA GTGGGCAGTG TTTCCATTTC CAGTGTCAGC
    ATCTCTGTGA GCGGCATGGT GATAACGCCC ACCTTCACTT ACAACCCTGC AACACAGGTG
    TTAAGCATTG ATGTGACCGG CAGAGATATC AGCCTCCATA ATTTCATTTC ACTGACGTGG
    AGAAGCACTC TGAATTTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_014119955.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product maltase-glucoamylase, intestinal
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975430.1
    CDS94..5595
    Translation

    Target ORF information:

    RefSeq Version XM_014119955.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119955.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_014119956.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975431.1
    CDS331..5832
    Translation

    Target ORF information:

    RefSeq Version XM_014119956.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119956.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_022403729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259437.1
    CDS285..5786
    Translation

    Target ORF information:

    RefSeq Version XM_022403729.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_005629542.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product maltase-glucoamylase, intestinal
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629599.1
    CDS66..5567
    Translation

    Target ORF information:

    RefSeq Version XM_005629542.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629542.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_014119954.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product maltase-glucoamylase, intestinal
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975429.1
    CDS200..5701
    Translation

    Target ORF information:

    RefSeq Version XM_014119954.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119954.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_014119956.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product maltase-glucoamylase, intestinal
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975431.1
    CDS177..5678
    Translation

    Target ORF information:

    RefSeq Version XM_014119956.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_014119957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product maltase-glucoamylase, intestinal
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975432.1
    CDS20..5521
    Translation

    Target ORF information:

    RefSeq Version XM_014119957.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17967
    Clone ID Related Accession (Same CDS sequence) XM_014119955.1 , XM_014119956.2 , XM_022403729.1 , XM_005629542.2 , XM_014119954.1 , XM_014119956.1 , XM_014119957.1 , XM_022403728.1
    Accession Version XM_022403728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259436.1
    CDS124..5625
    Translation

    Target ORF information:

    RefSeq Version XM_022403728.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA GGAGGTGGAA AGATTTCACG ACTTTGGAGC TTATGCTCAG TGTTCTTCTG 
    CTTATGGTGT TTATCATCAC TATTCCTCTG TTTGTGCTTT CAGCTCAAGA TTCTTTGGAA
    TCAAAAGATC CTGGGACAGT AGTTACCCCA GATCCTGGGA CAGCAAGTAC CACCCCTAAT
    TCTGCTGTGT GCCCGGTGGT AGATGAAGCA GAACGGATCA ACTGCATTCC TGACCAGCCA
    CCAACAAAGG CCACATGTGA TCAGCGTGGG TGCTGCTGGA GACCTCAGGG GACCATAAAT
    GTACCCTGGT GCTACTATTC TAGGAGTCAT GGTTATCAAG GGGAGGGTGA TGTTGTAAAA
    ACGAATGCAG GGTTCACAGC CCAGTTGAAG AGGTTGCCTT CTCCATCCCT ATTTGGAAAT
    GATGTCAACA ATGTCCTTCT CACAGCAGAG TATCAGACGT CCAATCGCTT CCACTTTAAG
    TTAACTGATC AGAGCAAAGA CAGGTATGAA GTGCCCCATG AACATGTGCA ACCCTTTAAA
    GGAGATGCTG CTTCACCCTT GACCTATGAA GTTGAGGTTT CCAAACAGCC ATTTAGCATC
    AAAGTAATCA GAACAAGCAA TAATCGTGTT CTGCTGGACT CAGGCATTGG ACCCCTCCTG
    TTTGCTGATC AGTTCCTGCA ATTCTCCACC CGACTGCCCA GTGCTAATGT GTACGGTCTG
    GGAGAACAGG TGCACCAACA GTACAGGCAT GATATGAATT GGAAGACCTG GCCTATATTT
    GCCCGGGACA CAACTCCCAA TGGGGATGGA ACTAACTTGT ATGGTGCACA GACATTCTTC
    CTGTGTCTTG AAGATGCTAG TGGATTGTCC TTTGGAGTGT TTCTAATGAA CAGCAATGCC
    ATGGAGGTTG CCCTCCAGCC CACCCCAGCC ATCACTTACC GCACCATCGG GGGCATTCTC
    GACTTCTATG TGTTTTTGGG AAACACTCCA GAGCAAGTTG TTCAAGAGTA TCTAGAGCTC
    ATTGGACGGC CAGCCCTTCC CTCATACTGG GCCCTTGGAT TTCATCTCAG TCGTTATGAT
    TATGGAACGC TAGAGAGCAT GAGGGAAGTC GTGCAGAGAA ACCGTGTGGC TCGGCTCCCT
    TATGACGTGC AGCATGCTGA TATTGATTAT ATGCATGAGA GGAAGGACTT CACTTATGAT
    CCAGTAAATT ATAAAGGCTT CCCTGAGTTT GTTAAGGAGT TACACAATAA CAGTCAGAAA
    TTGGTCATCA TTGTGGATCC AGCCATCTCC AACAACTCTT CCCCCAGCAA TCCTTACGGC
    CCATATGACA GAGGTTCAGG TGCAAAGATA TGGGTGAATG CTTCAGATGG GTTGACGCCG
    CTCATTGGAG AGGTCTGGCC TGGAAAAACT GTGTTTCCTG ATTATACCAA CCCTAGCTGT
    GCTGTTTGGT GGGCAAAGGA ATTTGAGCTT TTCCACAACA AAATAGAATT TGATGGAATC
    TGGATTGATA TGAATGAAGT CTCCAACTTT GTTGATGGTT CCATTTCAGG GTGTTCCACG
    AGCCATCTTA ATTATCCCCC ATTTGTTCCC AGAATCCTGG ATGGGTACCT GTTCTCCAAG
    AGTCTCTGCA TGGATGCAGT GCAGCATTGG GGCAAGCAGT ACGACGTCCA CAATCTGTAT
    GGCTACTCCA TGGCGATCGC CACGGCAGAA GCTGTCAAGA CCGTGTTCCC TAATAAGAGA
    AGCTTCATTT TAACCCGTTC TACCTTTGCG GGATCTGGCA AGTTTGCAGC CCACTGGTTA
    GGAGACAATG CGGCCACCTG GAATGATCTT CGGTGGTCTA TCCCTGGCAT GCTCGAGTTC
    AACCTTTTTG GTATCCCAAT GGTGGGTGCT GACATATGTG GCTTTGCACT GGACACCTCT
    GAGGAGCTCT GTAGGAGGTG GATGCAGCTG GGAGCATTTT ATCCATTTTC CAGGAATCAC
    AATGGCCAAG GCTACAAGGC CCAGGATCCT GCCTCTTTTG GAGCTGACTC TCTGCTGTTG
    AACTCCTCCA GGCACTACCT TAACATCCGT TACACTCTAT TACCCTACCT CTACACCCTC
    TTCTACCGTG CCCACAGCCG GGGGGACACA GTGGCCAGGC CCCTTATGCA TGAGTTCTAT
    GGGGATAGCA GTACATGGGA TGTACACCAA CAGTTTTTAT GGGGACCCGG CCTCCTCATC
    ACTCCAGTTC TGGATGAGGG TGCAGAGAAA GTGATGGCTT ATATACCCGA TGCCGTCTGG
    TACGATTATG AGACTGGGGC CCGAGCAAGA TGGAGGAAGC AAAGAGTGGA GATGGGACTT
    CCTGCAGACA AAATTGGACT TCACCTTCGC GGAGGCCACA TCTTTCCCAC ACAACAGCCA
    GCCACCACCA CGGTGGCCAG TCGCCGGAAC CCCCTTGGTC TTATCATTGC CCTGGATGAA
    AACAAAGAAG CAAAAGGCGA ACTTTTCTGG GATGATGGGG AAACTAAAGA TACTGTGGCC
    AATCAAGTTT ATCTCTTATG TGAGTTTTCT GTCACCCAAA ACCGCTTGGA TGTGAAGATT
    TTGCAGTCAA CTTACAAAGA CCCCAATAAT TTAGTATTTA AAGAGATTAA AATTCTTGGG
    ACCCAGGAAC CTACCAATGT TATAGTGAAA CACAATGGTA TCCCAAGTCA AACTTCTCCT
    AATGTCACTT ATGATTCTAA CCTACAGGTT GCTCTTATCA CAGAAATTGA TCTTATCCTC
    GGAGAAGCAT ACACGGTGGA GTGGGACCTG AAGATAAGGG ATGAAGAGAA AATAGACTGT
    TTTCCTGATG AGAGCGGTGC TTCTGCAGCA AACTGTACTG CCCGTGGCTG TGCCTGGGAG
    GCATCCAGTT CTCCTGGAGT CCCATTCTGT TATTTTGTCA ACGATCTGTA CTCTGTCAGC
    GATGTTCAGT ATGACTCACA TGGAGCCACT GCTACCATCT CCTTAAAATC TTCTGTCTAT
    GCCTCCGCTC TGCCCTCAGT GCCCGTGACT TCTCTCCGCC TACGTGTGAC CTACCATAAG
    AATGACATGC TACAGTTTAA GATTTATGAT CCCAATAATA ATCGGTATGA AGTCCCAGTC
    CCTCTCAACA TACCCAGGGT TCCATCCAGC ACCTCTGAGA GTCAACTCTA TGATGTCCTC
    ATTAAGAAGA ATCCATTTGG AATCGAAATT CGCCGGAAGA GTACGGGCAC TGTGATTTGG
    GACTCTCAGC TCCTTGGCTT TACCTTCAAT GACATGTTCA TCCGTATCTC CACTCGCCTT
    CCCTCCCAGT ACCTCTATGG TTTTGGGGAA ACTGAGCACA CAGCCTTTCG GAGAGACTTG
    AACTGGCACA CATGGGGGAT GTTCTCCAGG GACCAACCTC CGGGGTACAA GAAGAATTCC
    TATGGTGTCC ACCCCTACTA CATGGCACTG GAGGAGGATG GTAGTGCCCA TGGGGTGTTA
    CTGCTCAACA GCAATGCCAT GGATGTGACA TTCCAGCCCC TGCCTGCCTT GACATACCGC
    ACCATAGGAG GAATTCTGGA CTTTTATGTG TTCTTGGGGC CAACTCCAGA GCTTGTCACT
    CAGCAGTATA CTGAGATGAT TGGTCGGCCA GTGATGGTTC CTTACTGGTC CTTGGGGTTT
    CAGCTGTGTC GCTATGGCTA CCAGAACGAC TCTGAGATTG CCAGCCTGTA TGATGAGATG
    GTGGCTGCCC AGATCCCTTA TGATGTGCAG TACTCCGACA TCGACTACAT GGAGCGGCAG
    CTGGACTTCA CCCTCAGCCC CAAGTTCGCA GGATTTCCAG CTCTGATTAC TCGCATGAAG
    GCCAACGGAA TGCGGGTCAT CCTCATTCTG GACCCAGCCA TTTCTGGTAA TGAGACGCAG
    CCCTATCCTG CATTCACTCG GGGTGTGGAG GATGATGTCT TCATCAAAGC TCCAAATGGT
    GGAGGCATTG TCTGGGGGAA GGTGTGGCCT GATTTCCCTG ATGTCGTCAT AAACGCATCC
    CTAGACTGGG ATAGTCAAGT GGAGCTGTAC CGAGCTTATG TGGCCTTTCC TGACTTTTTC
    CGTAACTCCA CTACCACATG GTGGAAGAGG GAGCTGCAAG AACTATACAC CAACCCCCAG
    AATCCAGAGA GGAGCTTGAA GTTTGACGGC ATGTGGATTG ATATGAATGA GCCAGCAAGT
    TTTGTGAATG GAGCAGTCCC CCCAGGCTGC AGGGATGCCA CTATGAACCA CCCCCCCTAT
    ATGCCACATT TGGAGTCCAG GGATAAAGGC CTGAGCAGCA AGACCCTGTG CATGGAGAGC
    GAGCAGGTCC TGCCAGATGG CTCACGGGTG CGGCACTATG ATGTTCACAG CCTGTATGGA
    TGGGCCCAGA CCAGACCCAC CTACGAAGCT GTGCAGGAGG TGACGGGACA GAGAGGGATT
    GTCATCACCC GCTCCACATT CCCCTCTTCT GGCCGCTGGG GAGGCCACTG GCTGGGTGAC
    AACACGGCCG CGTGGGACCA GCTGAAGAAG TCTATCATTG GCATGATGGA GTTCAGCCTC
    TTCGGTATAT CTTATACAGG AGCAGATATT TGTGGGTTCT TTCAAGATGC TGAATATGAG
    ATGTGTGTTC GCTGGATGCA GCTGGGAGCC TTTTACCCCT TTTCGAGGAA CCACAACACC
    ATTGGGACCA GGAGGCAAGA CCCTGTGTCC TGGAATGCAA CCTTTGAGGA TATCTCCAGA
    AGTGTGCTGC AGACCAGATA CACCCTGTTG CCATATCTCT ACACCCTGAT GCACCTGGCC
    CACACGGAGG GCAGTACTGT CGTGCGGCCT CTTCTCCATG AGTTTGTGTC AGACCGGGTG
    ACCTGGAATG TAGACAGTCA GTTTCTGCTG GGCCCAGCCT TCCTAGTCAG CCCTGTCCTG
    GAGCCTAATG CCAGAAATGT CACTGCATAT TTCCCTAGAG CCCGCTGGTA TGATTACTAC
    ACGGGTGTGG ACATTGGAGC AAGGGCAGAG TGGAAGTCCT TGCCAGCCCC TCTTGACCAC
    ATTAACCTTC ATGTCCGTGG GGGCTACATC CTGCCCTGGC AAGAGCCTGC GCAGAACACC
    CACTTAAGCC GTCAGAAATT CATAGGCTTC AAGGTTGCTT TGGATGATGA GGGGGCTGCT
    AAAGGCTGGC TCTTCTGGGA TGATGGGCAA AGCATTGACA CCTATGGAAA AGGACACTAT
    TACCTGGCCA ACTTTTCTGC CAGCCAGAAT ATGATGCAGA GTCATATAGT TTTCAACAAA
    TACATCACCG ATGCAAACCC TTTGAAACTG GGCTACGTTG AGATCTGGGG AGTGGGCAGT
    GTTTCCATTT CCAGTGTCAG CATCTCTGTG AGCGGCATGG TGATAACGCC CACCTTCACT
    TACAACCCTG CAACACAGGT GTTAAGCATT GATGTGACCG GCAGAGATAT CAGCCTCCAT
    AATTTCATTT CACTGACGTG GAGAAGCACT CTGAATTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.