RGS22 cDNA ORF clone, Canis lupus familiaris(dog)

The following RGS22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RGS22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe292745 XM_022426563.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292746 XM_022426564.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292747 XM_022426566.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292748 XM_022426567.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292749 XM_022426568.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292750 XM_022426569.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292751 XM_022426570.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292752 XM_022426571.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292753 XM_022426572.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292754 XM_022426573.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292755 XM_022426574.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292756 XM_022426575.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe292757 XM_022426576.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292758 XM_022426577.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292759 XM_022426578.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292760 XM_022426579.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe292761 XM_022426580.1
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39625 XM_014118536.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39626 XM_014118537.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39627 XM_014118538.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39628 XM_014118539.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39629 XM_014118540.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39630 XM_014118541.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39631 XM_014118542.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39632 XM_014118543.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39633 XM_014118544.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39634 XM_014118545.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39635 XM_014118546.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39636 XM_014118547.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39637 XM_014118548.1
Latest version!
Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OCe39626 XM_014118537.1 Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe292745
    Clone ID Related Accession (Same CDS sequence) XM_022426563.1
    Accession Version XM_022426563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGAGCTGGAT CACAGTTGTG GTGCTAGCTG ATAATATCCC ACCACAAAGA
    TGGGACACCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282271.1
    CDS19..3993
    Translation

    Target ORF information:

    RefSeq Version XM_022426563.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGAGCTGGAT CACAGTTGTG GTGCTAGCTG ATAATATCCC ACCACAAAGA
    TGGGACACCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292746
    Clone ID Related Accession (Same CDS sequence) XM_022426564.1
    Accession Version XM_022426564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGAGAATTTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282272.1
    CDS19..3942
    Translation

    Target ORF information:

    RefSeq Version XM_022426564.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGAGAATTTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292747
    Clone ID Related Accession (Same CDS sequence) XM_022426566.1
    Accession Version XM_022426566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGC CTGTGGAGAG TAAATGGATA TCTTCATCTT GTGAAATCAT TGCTTTTCGT
    AAAGCATTAT TGAATCCAGT TACCGTAAGA CAATTTCAAC GGTTTGTGGC TCTAAAAGGA
    GATTTATTGG AAAATGATGT ACTCTTTTGG CAAGAAGTAC AAAAATATAA GGACTTGTGC
    CATTCTCATT GTGAAGAGTC CATCATCCAT AAGAAGATCA TGACTATCAT CAACTGCTTT
    ATTAATTCTA GTATTCCACC AGCTTTACAA ATTGACATTC CAGTAGAGCA AGCCCAAAAA
    ATTATTGAAC ACAGGAAGGA GTTAGGACCA TATGTGTTTA GAGAGGCACA GATGACAATT
    TTTGGGGTTC TGTTTAAATT TTGGCCTCAG TTTTGTGAGT TTAGGAAGAA CTTAACTGAT
    GAAAAAATTA TGAGTGTTTT AGAGAGAAGA CAAGAATATA ATAAGCAGAA AAAAAAATTG
    GCAGTCATAG AAGACGAAAA TTTTGCAAAG GATGGAATCA AACAATATCC AAGTTCTTCA
    GGATCTGCAA TCAAAACAGC TGCTTTAGCC ACTGACCCAG TTTTAGGCCT ACAAGCATAT
    GGCCGGCAGC CAACCTGGTG CTACTCCAAG TACATAGAAG CCTTAGAGCA AGAAAGGATT
    CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA AAGTTATTTA CAGCCACACC ATCTTTTACA
    AACTTCAAGT CTACTGCTTC CACTATGTCT TTGAAAAAGA CCGTATCTTC CCACAACATC
    CAGAGCTGGA TCACAGTTGT GGTGCTAGCT GATAATATCC CACCACAAAG ATGGGACACC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282274.1
    CDS19..3924
    Translation

    Target ORF information:

    RefSeq Version XM_022426566.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGC CTGTGGAGAG TAAATGGATA TCTTCATCTT GTGAAATCAT TGCTTTTCGT
    AAAGCATTAT TGAATCCAGT TACCGTAAGA CAATTTCAAC GGTTTGTGGC TCTAAAAGGA
    GATTTATTGG AAAATGATGT ACTCTTTTGG CAAGAAGTAC AAAAATATAA GGACTTGTGC
    CATTCTCATT GTGAAGAGTC CATCATCCAT AAGAAGATCA TGACTATCAT CAACTGCTTT
    ATTAATTCTA GTATTCCACC AGCTTTACAA ATTGACATTC CAGTAGAGCA AGCCCAAAAA
    ATTATTGAAC ACAGGAAGGA GTTAGGACCA TATGTGTTTA GAGAGGCACA GATGACAATT
    TTTGGGGTTC TGTTTAAATT TTGGCCTCAG TTTTGTGAGT TTAGGAAGAA CTTAACTGAT
    GAAAAAATTA TGAGTGTTTT AGAGAGAAGA CAAGAATATA ATAAGCAGAA AAAAAAATTG
    GCAGTCATAG AAGACGAAAA TTTTGCAAAG GATGGAATCA AACAATATCC AAGTTCTTCA
    GGATCTGCAA TCAAAACAGC TGCTTTAGCC ACTGACCCAG TTTTAGGCCT ACAAGCATAT
    GGCCGGCAGC CAACCTGGTG CTACTCCAAG TACATAGAAG CCTTAGAGCA AGAAAGGATT
    CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA AAGTTATTTA CAGCCACACC ATCTTTTACA
    AACTTCAAGT CTACTGCTTC CACTATGTCT TTGAAAAAGA CCGTATCTTC CCACAACATC
    CAGAGCTGGA TCACAGTTGT GGTGCTAGCT GATAATATCC CACCACAAAG ATGGGACACC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292748
    Clone ID Related Accession (Same CDS sequence) XM_022426567.1
    Accession Version XM_022426567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATACAGATGA GAGACATAGC TGAAGAGCTC
    TTATTACAGA AGCATGAGAG GAAAATCGGG GTTTGGAAGC CTGTGGAGAG TAAATGGATA
    TCTTCATCTT GTGAAATCAT TGCTTTTCGT AAAGCATTAT TGAATCCAGT TACCGTAAGA
    CAATTTCAAC GGTTTGTGGC TCTAAAAGGA GATTTATTGG AAAATGATGT ACTCTTTTGG
    CAAGAAGTAC AAAAATATAA GGACTTGTGC CATTCTCATT GTGAAGAGTC CATCATCCAT
    AAGAAGATCA TGACTATCAT CAACTGCTTT ATTAATTCTA GTATTCCACC AGCTTTACAA
    ATTGACATTC CAGTAGAGCA AGCCCAAAAA ATTATTGAAC ACAGGAAGGA GTTAGGACCA
    TATGTGTTTA GAGAGGCACA GATGACAATT TTTGGGGTTC TGTTTAAATT TTGGCCTCAG
    TTTTGTGAGT TTAGGAAGAA CTTAACTGAT GAAAAAATTA TGAGTGTTTT AGAGAGAAGA
    CAAGAATATA ATAAGCAGAA AAAAAAATTG GCAGTCATAG AAGACGAAAA TTTTGCAAAG
    GATGGAATCA AACAATATCC AAGTTCTTCA GGATCTGCAA TCAAAACAGC TGCTTTAGCC
    ACTGACCCAG TTTTAGGCCT ACAAGCATAT GGCCGGCAGC CAACCTGGTG CTACTCCAAG
    TACATAGAAG CCTTAGAGCA AGAAAGGATT CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA
    AAGTTATTTA CAGCCACACC ATCTTTTACA AACTTCAAGT CTACTGCTTC CACTATGTCT
    TTGAAAAAGA CCGTATCTTC CCACAACATC CAGAGCTGGA TCACAGTTGT GGTGCTAGCT
    GATAATATCC CACCACAAAG ATGGGACACC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282275.1
    CDS19..3834
    Translation

    Target ORF information:

    RefSeq Version XM_022426567.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATACAGATGA GAGACATAGC TGAAGAGCTC
    TTATTACAGA AGCATGAGAG GAAAATCGGG GTTTGGAAGC CTGTGGAGAG TAAATGGATA
    TCTTCATCTT GTGAAATCAT TGCTTTTCGT AAAGCATTAT TGAATCCAGT TACCGTAAGA
    CAATTTCAAC GGTTTGTGGC TCTAAAAGGA GATTTATTGG AAAATGATGT ACTCTTTTGG
    CAAGAAGTAC AAAAATATAA GGACTTGTGC CATTCTCATT GTGAAGAGTC CATCATCCAT
    AAGAAGATCA TGACTATCAT CAACTGCTTT ATTAATTCTA GTATTCCACC AGCTTTACAA
    ATTGACATTC CAGTAGAGCA AGCCCAAAAA ATTATTGAAC ACAGGAAGGA GTTAGGACCA
    TATGTGTTTA GAGAGGCACA GATGACAATT TTTGGGGTTC TGTTTAAATT TTGGCCTCAG
    TTTTGTGAGT TTAGGAAGAA CTTAACTGAT GAAAAAATTA TGAGTGTTTT AGAGAGAAGA
    CAAGAATATA ATAAGCAGAA AAAAAAATTG GCAGTCATAG AAGACGAAAA TTTTGCAAAG
    GATGGAATCA AACAATATCC AAGTTCTTCA GGATCTGCAA TCAAAACAGC TGCTTTAGCC
    ACTGACCCAG TTTTAGGCCT ACAAGCATAT GGCCGGCAGC CAACCTGGTG CTACTCCAAG
    TACATAGAAG CCTTAGAGCA AGAAAGGATT CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA
    AAGTTATTTA CAGCCACACC ATCTTTTACA AACTTCAAGT CTACTGCTTC CACTATGTCT
    TTGAAAAAGA CCGTATCTTC CCACAACATC CAGAGCTGGA TCACAGTTGT GGTGCTAGCT
    GATAATATCC CACCACAAAG ATGGGACACC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292749
    Clone ID Related Accession (Same CDS sequence) XM_022426568.1
    Accession Version XM_022426568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    GATGGAATCA AACAATATCC AAGTTCTTCA GGATCTGCAA TCAAAACAGC TGCTTTAGCC
    ACTGACCCAG TTTTAGGCCT ACAAGCATAT GGCCGGCAGC CAACCTGGTG CTACTCCAAG
    TACATAGAAG CCTTAGAGCA AGAAAGGATT CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA
    AAGTTATTTA CAGCCACACC ATCTTTTACA AACTTCAAGT CTACTGCTTC CACTATGTCT
    TTGAAAAAGA CCGTATCTTC CCACAACATC CAGAGCTGGA TCACAGTTGT GGTGCTAGCT
    GATAATATCC CACCACAAAG ATGGGACACC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282276.1
    CDS19..3834
    Translation

    Target ORF information:

    RefSeq Version XM_022426568.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    GATGGAATCA AACAATATCC AAGTTCTTCA GGATCTGCAA TCAAAACAGC TGCTTTAGCC
    ACTGACCCAG TTTTAGGCCT ACAAGCATAT GGCCGGCAGC CAACCTGGTG CTACTCCAAG
    TACATAGAAG CCTTAGAGCA AGAAAGGATT CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA
    AAGTTATTTA CAGCCACACC ATCTTTTACA AACTTCAAGT CTACTGCTTC CACTATGTCT
    TTGAAAAAGA CCGTATCTTC CCACAACATC CAGAGCTGGA TCACAGTTGT GGTGCTAGCT
    GATAATATCC CACCACAAAG ATGGGACACC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292750
    Clone ID Related Accession (Same CDS sequence) XM_022426569.1
    Accession Version XM_022426569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGGGCAC CAAGATTCTG TGTTACTCAT
    TCATCCTCAG CTAAACATGC TAGCGCTGAA CTGAAATTCT GGCATCTCCG TCAAGAGAAA
    CCAAGGAAGG ACGTTGACCC TTTTCCACAA ATGGCAACCC TCTTGCCATT AAGACCTAAA
    TCTTGCATTC CACAGATATC TGATATGCAG AAAGAAGAAT CCAACTTATT ACAATGTCCT
    AAATCTCCCA AGAAACCACC TAGAGTAAAA ACAGCAGGTC AAAAACCTTG GAAGAGCAAG
    TCTTTGTATC TAATTTCTTC GAAGGATGAT GCAATTGAGA AAGGGTTGAG GCACAGGTCA
    GAAAGCAACA AAGTTATTCG CTTAACATCT TTTACTGACA TTTCTGAATG TCTGAAGCCC
    CAGCTGGATA GAAGATACAC TTACACAGAA GAACCTTCGG TTAAAACCAT GACAGATGGT
    GCCTTGGGAG GATTTGACAT GGAAAATTTG TTGCAATCTT TGTATGTAGA AAATAGAGCT
    GGATTCTTCT TTACTAAATT CTGTGAGCAT TCAGGAAACA AGTTGTGGAA GAACAGTGTG
    TACTTTTGGT TTGACCTACA GGCTTATCAT CAGCTTTTCT ACCAAGAAAC ACTTCACCCT
    TTTAAAGTAT GCAAACAAGC CCAATATCTT TTTGCCACAT ACATTGCTCC TTCGGCTACT
    CTTGACATCG GACTCCAACA GGAAAAGAAA AAAGAAATTT ATATGAAAAT ACAGCCACCA
    TTTGAAGACC TTTTTGACAC AGCAGAAGAA TATATCCTTC TCCTCCTCCT TGAGCCGTGG
    ACAAAGATGG TAAAATTAGA CCAAGTTGCT TTTGGAAAGG TGGAGCTGGT GGAAGACACT
    CGACAGCTAG ACTCCACATA CTTCAGAAAG CTACAGGCTT TGCATAAAGA GACCTTTTCC
    AAGAAAGCTG AGGACACTAC TTGTGAAATT GGAACCGGTA TTTCACGGCT TCCTGAGGTC
    TCTAAAAAAA CAGAATACTG GAGTAATGTT CCTGAAGAAT ATAGGCACTT TAATTTTAAT
    GACCTGCTTA ACAACAAACT GGAGTTTGAA CATTTCCGTC AGTTTCTTGA GGCTCATTCT
    TCAAGTTTGG ACCTTATGTG CTGGACAGAC ATTGAACAGT TCCGAAGAAT CATTTATGGA
    GACCGAAAGC AGAGGGAAGC AAAATCTATA TATATTAAAA ACAAATACCT TAATAAAAAA
    TATTTCTTTG GCCCCCAAAG TCCAGCTTCT CAATATCAGC AGGACCAGAT AATGAATTTA
    AGTGGTGGCT GGGGACAGAT GCTCCATGAG CAGCTTGATG CACCTGTTCT AATTGAAGTT
    CAGAAGCATG TTCTCAATAA GCTAGAAAAT GTGTGGTTGC CATTGTTTCT TGCAAGCGAA
    CAGTTTGTAG CACGTCAAAA GATAAAGATA CAGATGAGAG ACATAGCTGA AGAGCTCTTA
    TTACAGAAGC ATGAGAGGAA AATCGGGGTT TGGAAGCCTG TGGAGAGTAA ATGGATATCT
    TCATCTTGTG AAATCATTGC TTTTCGTAAA GCATTATTGA ATCCAGTTAC CGTAAGACAA
    TTTCAACGGT TTGTGGCTCT AAAAGGAGAT TTATTGGAAA ATGATGTACT CTTTTGGCAA
    GAAGTACAAA AATATAAGGA CTTGTGCCAT TCTCATTGTG AAGAGTCCAT CATCCATAAG
    AAGATCATGA CTATCATCAA CTGCTTTATT AATTCTAGTA TTCCACCAGC TTTACAAATT
    GACATTCCAG TAGAGCAAGC CCAAAAAATT ATTGAACACA GGAAGGAGTT AGGACCATAT
    GTGTTTAGAG AGGCACAGAT GACAATTTTT GGGGTTCTGT TTAAATTTTG GCCTCAGTTT
    TGTGAGTTTA GGAAGAACTT AACTGATGAA AAAATTATGA GTGTTTTAGA GAGAAGACAA
    GAATATAATA AGCAGAAAAA AAAATTGGCA GTCATAGAAG ACGAAAATTT TGCAAAGGAT
    GGAATCAAAC AATATCCAAG TTCTTCAGGA TCTGCAATCA AAACAGCTGC TTTAGCCACT
    GACCCAGTTT TAGGCCTACA AGCATATGGC CGGCAGCCAA CCTGGTGCTA CTCCAAGTAC
    ATAGAAGCCT TAGAGCAAGA AAGGATTCTT CTTAAAATTC AAGAGGAGCT GGAAAAAAAG
    TTATTTACAG CCACACCATC TTTTACAAAC TTCAAGTCTA CTGCTTCCAC TATGTCTTTG
    AAAAAGACCG TATCTTCCCA CAACATCCAG AGCTGGATCA CAGTTGTGGT GCTAGCTGAT
    AATATCCCAC CACAAAGATG GGACACCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282277.1
    CDS19..3831
    Translation

    Target ORF information:

    RefSeq Version XM_022426569.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGGGCAC CAAGATTCTG TGTTACTCAT
    TCATCCTCAG CTAAACATGC TAGCGCTGAA CTGAAATTCT GGCATCTCCG TCAAGAGAAA
    CCAAGGAAGG ACGTTGACCC TTTTCCACAA ATGGCAACCC TCTTGCCATT AAGACCTAAA
    TCTTGCATTC CACAGATATC TGATATGCAG AAAGAAGAAT CCAACTTATT ACAATGTCCT
    AAATCTCCCA AGAAACCACC TAGAGTAAAA ACAGCAGGTC AAAAACCTTG GAAGAGCAAG
    TCTTTGTATC TAATTTCTTC GAAGGATGAT GCAATTGAGA AAGGGTTGAG GCACAGGTCA
    GAAAGCAACA AAGTTATTCG CTTAACATCT TTTACTGACA TTTCTGAATG TCTGAAGCCC
    CAGCTGGATA GAAGATACAC TTACACAGAA GAACCTTCGG TTAAAACCAT GACAGATGGT
    GCCTTGGGAG GATTTGACAT GGAAAATTTG TTGCAATCTT TGTATGTAGA AAATAGAGCT
    GGATTCTTCT TTACTAAATT CTGTGAGCAT TCAGGAAACA AGTTGTGGAA GAACAGTGTG
    TACTTTTGGT TTGACCTACA GGCTTATCAT CAGCTTTTCT ACCAAGAAAC ACTTCACCCT
    TTTAAAGTAT GCAAACAAGC CCAATATCTT TTTGCCACAT ACATTGCTCC TTCGGCTACT
    CTTGACATCG GACTCCAACA GGAAAAGAAA AAAGAAATTT ATATGAAAAT ACAGCCACCA
    TTTGAAGACC TTTTTGACAC AGCAGAAGAA TATATCCTTC TCCTCCTCCT TGAGCCGTGG
    ACAAAGATGG TAAAATTAGA CCAAGTTGCT TTTGGAAAGG TGGAGCTGGT GGAAGACACT
    CGACAGCTAG ACTCCACATA CTTCAGAAAG CTACAGGCTT TGCATAAAGA GACCTTTTCC
    AAGAAAGCTG AGGACACTAC TTGTGAAATT GGAACCGGTA TTTCACGGCT TCCTGAGGTC
    TCTAAAAAAA CAGAATACTG GAGTAATGTT CCTGAAGAAT ATAGGCACTT TAATTTTAAT
    GACCTGCTTA ACAACAAACT GGAGTTTGAA CATTTCCGTC AGTTTCTTGA GGCTCATTCT
    TCAAGTTTGG ACCTTATGTG CTGGACAGAC ATTGAACAGT TCCGAAGAAT CATTTATGGA
    GACCGAAAGC AGAGGGAAGC AAAATCTATA TATATTAAAA ACAAATACCT TAATAAAAAA
    TATTTCTTTG GCCCCCAAAG TCCAGCTTCT CAATATCAGC AGGACCAGAT AATGAATTTA
    AGTGGTGGCT GGGGACAGAT GCTCCATGAG CAGCTTGATG CACCTGTTCT AATTGAAGTT
    CAGAAGCATG TTCTCAATAA GCTAGAAAAT GTGTGGTTGC CATTGTTTCT TGCAAGCGAA
    CAGTTTGTAG CACGTCAAAA GATAAAGATA CAGATGAGAG ACATAGCTGA AGAGCTCTTA
    TTACAGAAGC ATGAGAGGAA AATCGGGGTT TGGAAGCCTG TGGAGAGTAA ATGGATATCT
    TCATCTTGTG AAATCATTGC TTTTCGTAAA GCATTATTGA ATCCAGTTAC CGTAAGACAA
    TTTCAACGGT TTGTGGCTCT AAAAGGAGAT TTATTGGAAA ATGATGTACT CTTTTGGCAA
    GAAGTACAAA AATATAAGGA CTTGTGCCAT TCTCATTGTG AAGAGTCCAT CATCCATAAG
    AAGATCATGA CTATCATCAA CTGCTTTATT AATTCTAGTA TTCCACCAGC TTTACAAATT
    GACATTCCAG TAGAGCAAGC CCAAAAAATT ATTGAACACA GGAAGGAGTT AGGACCATAT
    GTGTTTAGAG AGGCACAGAT GACAATTTTT GGGGTTCTGT TTAAATTTTG GCCTCAGTTT
    TGTGAGTTTA GGAAGAACTT AACTGATGAA AAAATTATGA GTGTTTTAGA GAGAAGACAA
    GAATATAATA AGCAGAAAAA AAAATTGGCA GTCATAGAAG ACGAAAATTT TGCAAAGGAT
    GGAATCAAAC AATATCCAAG TTCTTCAGGA TCTGCAATCA AAACAGCTGC TTTAGCCACT
    GACCCAGTTT TAGGCCTACA AGCATATGGC CGGCAGCCAA CCTGGTGCTA CTCCAAGTAC
    ATAGAAGCCT TAGAGCAAGA AAGGATTCTT CTTAAAATTC AAGAGGAGCT GGAAAAAAAG
    TTATTTACAG CCACACCATC TTTTACAAAC TTCAAGTCTA CTGCTTCCAC TATGTCTTTG
    AAAAAGACCG TATCTTCCCA CAACATCCAG AGCTGGATCA CAGTTGTGGT GCTAGCTGAT
    AATATCCCAC CACAAAGATG GGACACCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292751
    Clone ID Related Accession (Same CDS sequence) XM_022426570.1
    Accession Version XM_022426570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGGA CTTGTGCCAT TCTCATTGTG AAGAGTCCAT CATCCATAAG
    AAGATCATGA CTATCATCAA CTGCTTTATT AATTCTAGTA TTCCACCAGC TTTACAAATT
    GACATTCCAG TAGAGCAAGC CCAAAAAATT ATTGAACACA GGAAGGAGTT AGGACCATAT
    GTGTTTAGAG AGGCACAGAT GACAATTTTT GGGGTTCTGT TTAAATTTTG GCCTCAGTTT
    TGTGAGTTTA GGAAGAACTT AACTGATGAA AAAATTATGA GTGTTTTAGA GAGAAGACAA
    GAATATAATA AGCAGAAAAA AAAATTGGCA GTCATAGAAG ACGAAAATTT TGCAAAGGAT
    GGAATCAAAC AATATCCAAG TTCTTCAGGA TCTGCAATCA AAACAGCTGC TTTAGCCACT
    GACCCAGTTT TAGGCCTACA AGCATATGGC CGGCAGCCAA CCTGGTGCTA CTCCAAGTAC
    ATAGAAGCCT TAGAGCAAGA AAGGATTCTT CTTAAAATTC AAGAGGAGCT GGAAAAAAAG
    TTATTTACAG CCACACCATC TTTTACAAAC TTCAAGTCTA CTGCTTCCAC TATGTCTTTG
    AAAAAGACCG TATCTTCCCA CAACATCCAG AGCTGGATCA CAGTTGTGGT GCTAGCTGAT
    AATATCCCAC CACAAAGATG GGACACCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282278.1
    CDS19..3831
    Translation

    Target ORF information:

    RefSeq Version XM_022426570.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGGA CTTGTGCCAT TCTCATTGTG AAGAGTCCAT CATCCATAAG
    AAGATCATGA CTATCATCAA CTGCTTTATT AATTCTAGTA TTCCACCAGC TTTACAAATT
    GACATTCCAG TAGAGCAAGC CCAAAAAATT ATTGAACACA GGAAGGAGTT AGGACCATAT
    GTGTTTAGAG AGGCACAGAT GACAATTTTT GGGGTTCTGT TTAAATTTTG GCCTCAGTTT
    TGTGAGTTTA GGAAGAACTT AACTGATGAA AAAATTATGA GTGTTTTAGA GAGAAGACAA
    GAATATAATA AGCAGAAAAA AAAATTGGCA GTCATAGAAG ACGAAAATTT TGCAAAGGAT
    GGAATCAAAC AATATCCAAG TTCTTCAGGA TCTGCAATCA AAACAGCTGC TTTAGCCACT
    GACCCAGTTT TAGGCCTACA AGCATATGGC CGGCAGCCAA CCTGGTGCTA CTCCAAGTAC
    ATAGAAGCCT TAGAGCAAGA AAGGATTCTT CTTAAAATTC AAGAGGAGCT GGAAAAAAAG
    TTATTTACAG CCACACCATC TTTTACAAAC TTCAAGTCTA CTGCTTCCAC TATGTCTTTG
    AAAAAGACCG TATCTTCCCA CAACATCCAG AGCTGGATCA CAGTTGTGGT GCTAGCTGAT
    AATATCCCAC CACAAAGATG GGACACCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292752
    Clone ID Related Accession (Same CDS sequence) XM_022426571.1
    Accession Version XM_022426571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAAGTGG AGCTGGTGGA AGACACTCGA CAGCTAGACT CCACATACTT CAGAAAGCTA
    CAGGCTTTGC ATAAAGAGAC CTTTTCCAAG AAAGCTGAGG ACACTACTTG TGAAATTGGA
    ACCGGTATTT CACGGCTTCC TGAGGTCTCT AAAAAAACAG AATACTGGAG TAATGTTCCT
    GAAGAATATA GGCACTTTAA TTTTAATGAC CTGCTTAACA ACAAACTGGA GTTTGAACAT
    TTCCGTCAGT TTCTTGAGGC TCATTCTTCA AGTTTGGACC TTATGTGCTG GACAGACATT
    GAACAGTTCC GAAGAATCAT TTATGGAGAC CGAAAGCAGA GGGAAGCAAA ATCTATATAT
    ATTAAAAACA AATACCTTAA TAAAAAATAT TTCTTTGGCC CCCAAAGTCC AGCTTCTCAA
    TATCAGCAGG ACCAGATAAT GAATTTAAGT GGTGGCTGGG GACAGATGCT CCATGAGCAG
    CTTGATGCAC CTGTTCTAAT TGAAGTTCAG AAGCATGTTC TCAATAAGCT AGAAAATGTG
    TGGTTGCCAT TGTTTCTTGC AAGCGAACAG TTTGTAGCAC GTCAAAAGAT AAAGATACAG
    ATGAGAGACA TAGCTGAAGA GCTCTTATTA CAGAAGCATG AGAGGAAAAT CGGGGTTTGG
    AAGCCTGTGG AGAGTAAATG GATATCTTCA TCTTGTGAAA TCATTGCTTT TCGTAAAGCA
    TTATTGAATC CAGTTACCGT AAGACAATTT CAACGGTTTG TGGCTCTAAA AGGAGATTTA
    TTGGAAAATG ATGTACTCTT TTGGCAAGAA GTACAAAAAT ATAAGGACTT GTGCCATTCT
    CATTGTGAAG AGTCCATCAT CCATAAGAAG ATCATGACTA TCATCAACTG CTTTATTAAT
    TCTAGTATTC CACCAGCTTT ACAAATTGAC ATTCCAGTAG AGCAAGCCCA AAAAATTATT
    GAACACAGGA AGGAGTTAGG ACCATATGTG TTTAGAGAGG CACAGATGAC AATTTTTGGG
    GTTCTGTTTA AATTTTGGCC TCAGTTTTGT GAGTTTAGGA AGAACTTAAC TGATGAAAAA
    ATTATGAGTG TTTTAGAGAG AAGACAAGAA TATAATAAGC AGAAAAAAAA ATTGGCAGTC
    ATAGAAGACG AAAATTTTGC AAAGGATGGA ATCAAACAAT ATCCAAGTTC TTCAGGATCT
    GCAATCAAAA CAGCTGCTTT AGCCACTGAC CCAGTTTTAG GCCTACAAGC ATATGGCCGG
    CAGCCAACCT GGTGCTACTC CAAGTACATA GAAGCCTTAG AGCAAGAAAG GATTCTTCTT
    AAAATTCAAG AGGAGCTGGA AAAAAAGTTA TTTACAGCCA CACCATCTTT TACAAACTTC
    AAGTCTACTG CTTCCACTAT GTCTTTGAAA AAGACCGTAT CTTCCCACAA CATCCAGAGC
    TGGATCACAG TTGTGGTGCT AGCTGATAAT ATCCCACCAC AAAGATGGGA CACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282279.1
    CDS19..3798
    Translation

    Target ORF information:

    RefSeq Version XM_022426571.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAAGTGG AGCTGGTGGA AGACACTCGA CAGCTAGACT CCACATACTT CAGAAAGCTA
    CAGGCTTTGC ATAAAGAGAC CTTTTCCAAG AAAGCTGAGG ACACTACTTG TGAAATTGGA
    ACCGGTATTT CACGGCTTCC TGAGGTCTCT AAAAAAACAG AATACTGGAG TAATGTTCCT
    GAAGAATATA GGCACTTTAA TTTTAATGAC CTGCTTAACA ACAAACTGGA GTTTGAACAT
    TTCCGTCAGT TTCTTGAGGC TCATTCTTCA AGTTTGGACC TTATGTGCTG GACAGACATT
    GAACAGTTCC GAAGAATCAT TTATGGAGAC CGAAAGCAGA GGGAAGCAAA ATCTATATAT
    ATTAAAAACA AATACCTTAA TAAAAAATAT TTCTTTGGCC CCCAAAGTCC AGCTTCTCAA
    TATCAGCAGG ACCAGATAAT GAATTTAAGT GGTGGCTGGG GACAGATGCT CCATGAGCAG
    CTTGATGCAC CTGTTCTAAT TGAAGTTCAG AAGCATGTTC TCAATAAGCT AGAAAATGTG
    TGGTTGCCAT TGTTTCTTGC AAGCGAACAG TTTGTAGCAC GTCAAAAGAT AAAGATACAG
    ATGAGAGACA TAGCTGAAGA GCTCTTATTA CAGAAGCATG AGAGGAAAAT CGGGGTTTGG
    AAGCCTGTGG AGAGTAAATG GATATCTTCA TCTTGTGAAA TCATTGCTTT TCGTAAAGCA
    TTATTGAATC CAGTTACCGT AAGACAATTT CAACGGTTTG TGGCTCTAAA AGGAGATTTA
    TTGGAAAATG ATGTACTCTT TTGGCAAGAA GTACAAAAAT ATAAGGACTT GTGCCATTCT
    CATTGTGAAG AGTCCATCAT CCATAAGAAG ATCATGACTA TCATCAACTG CTTTATTAAT
    TCTAGTATTC CACCAGCTTT ACAAATTGAC ATTCCAGTAG AGCAAGCCCA AAAAATTATT
    GAACACAGGA AGGAGTTAGG ACCATATGTG TTTAGAGAGG CACAGATGAC AATTTTTGGG
    GTTCTGTTTA AATTTTGGCC TCAGTTTTGT GAGTTTAGGA AGAACTTAAC TGATGAAAAA
    ATTATGAGTG TTTTAGAGAG AAGACAAGAA TATAATAAGC AGAAAAAAAA ATTGGCAGTC
    ATAGAAGACG AAAATTTTGC AAAGGATGGA ATCAAACAAT ATCCAAGTTC TTCAGGATCT
    GCAATCAAAA CAGCTGCTTT AGCCACTGAC CCAGTTTTAG GCCTACAAGC ATATGGCCGG
    CAGCCAACCT GGTGCTACTC CAAGTACATA GAAGCCTTAG AGCAAGAAAG GATTCTTCTT
    AAAATTCAAG AGGAGCTGGA AAAAAAGTTA TTTACAGCCA CACCATCTTT TACAAACTTC
    AAGTCTACTG CTTCCACTAT GTCTTTGAAA AAGACCGTAT CTTCCCACAA CATCCAGAGC
    TGGATCACAG TTGTGGTGCT AGCTGATAAT ATCCCACCAC AAAGATGGGA CACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292753
    Clone ID Related Accession (Same CDS sequence) XM_022426572.1
    Accession Version XM_022426572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGG ACTTGTGCCA TTCTCATTGT GAAGAGTCCA TCATCCATAA GAAGATCATG
    ACTATCATCA ACTGCTTTAT TAATTCTAGT ATTCCACCAG CTTTACAAAT TGACATTCCA
    GTAGAGCAAG CCCAAAAAAT TATTGAACAC AGGAAGGAGT TAGGACCATA TGTGTTTAGA
    GAGGCACAGA TGACAATTTT TGGGGTTCTG TTTAAATTTT GGCCTCAGTT TTGTGAGTTT
    AGGAAGAACT TAACTGATGA AAAAATTATG AGTGTTTTAG AGAGAAGACA AGAATATAAT
    AAGCAGAAAA AAAAATTGGC AGTCATAGAA GACGAAAATT TTGCAAAGGA TGGAATCAAA
    CAATATCCAA GTTCTTCAGG ATCTGCAATC AAAACAGCTG CTTTAGCCAC TGACCCAGTT
    TTAGGCCTAC AAGCATATGG CCGGCAGCCA ACCTGGTGCT ACTCCAAGTA CATAGAAGCC
    TTAGAGCAAG AAAGGATTCT TCTTAAAATT CAAGAGGAGC TGGAAAAAAA GTTATTTACA
    GCCACACCAT CTTTTACAAA CTTCAAGTCT ACTGCTTCCA CTATGTCTTT GAAAAAGACC
    GTATCTTCCC ACAACATCCA GAGCTGGATC ACAGTTGTGG TGCTAGCTGA TAATATCCCA
    CCACAAAGAT GGGACACCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282280.1
    CDS19..3762
    Translation

    Target ORF information:

    RefSeq Version XM_022426572.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGG ACTTGTGCCA TTCTCATTGT GAAGAGTCCA TCATCCATAA GAAGATCATG
    ACTATCATCA ACTGCTTTAT TAATTCTAGT ATTCCACCAG CTTTACAAAT TGACATTCCA
    GTAGAGCAAG CCCAAAAAAT TATTGAACAC AGGAAGGAGT TAGGACCATA TGTGTTTAGA
    GAGGCACAGA TGACAATTTT TGGGGTTCTG TTTAAATTTT GGCCTCAGTT TTGTGAGTTT
    AGGAAGAACT TAACTGATGA AAAAATTATG AGTGTTTTAG AGAGAAGACA AGAATATAAT
    AAGCAGAAAA AAAAATTGGC AGTCATAGAA GACGAAAATT TTGCAAAGGA TGGAATCAAA
    CAATATCCAA GTTCTTCAGG ATCTGCAATC AAAACAGCTG CTTTAGCCAC TGACCCAGTT
    TTAGGCCTAC AAGCATATGG CCGGCAGCCA ACCTGGTGCT ACTCCAAGTA CATAGAAGCC
    TTAGAGCAAG AAAGGATTCT TCTTAAAATT CAAGAGGAGC TGGAAAAAAA GTTATTTACA
    GCCACACCAT CTTTTACAAA CTTCAAGTCT ACTGCTTCCA CTATGTCTTT GAAAAAGACC
    GTATCTTCCC ACAACATCCA GAGCTGGATC ACAGTTGTGG TGCTAGCTGA TAATATCCCA
    CCACAAAGAT GGGACACCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292754
    Clone ID Related Accession (Same CDS sequence) XM_022426573.1
    Accession Version XM_022426573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATACAGATGA GAGACATAGC TGAAGAGCTC
    TTATTACAGA AGCATGAGAG GAAAATCGGG GTTTGGAAGG ACTTGTGCCA TTCTCATTGT
    GAAGAGTCCA TCATCCATAA GAAGATCATG ACTATCATCA ACTGCTTTAT TAATTCTAGT
    ATTCCACCAG CTTTACAAAT TGACATTCCA GTAGAGCAAG CCCAAAAAAT TATTGAACAC
    AGGAAGGAGT TAGGACCATA TGTGTTTAGA GAGGCACAGA TGACAATTTT TGGGGTTCTG
    TTTAAATTTT GGCCTCAGTT TTGTGAGTTT AGGAAGAACT TAACTGATGA AAAAATTATG
    AGTGTTTTAG AGAGAAGACA AGAATATAAT AAGCAGAAAA AAAAATTGGC AGTCATAGAA
    GACGAAAATT TTGCAAAGGA TGGAATCAAA CAATATCCAA GTTCTTCAGG ATCTGCAATC
    AAAACAGCTG CTTTAGCCAC TGACCCAGTT TTAGGCCTAC AAGCATATGG CCGGCAGCCA
    ACCTGGTGCT ACTCCAAGTA CATAGAAGCC TTAGAGCAAG AAAGGATTCT TCTTAAAATT
    CAAGAGGAGC TGGAAAAAAA GTTATTTACA GCCACACCAT CTTTTACAAA CTTCAAGTCT
    ACTGCTTCCA CTATGTCTTT GAAAAAGACC GTATCTTCCC ACAACATCCA GAGCTGGATC
    ACAGTTGTGG TGCTAGCTGA TAATATCCCA CCACAAAGAT GGGACACCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282281.1
    CDS19..3672
    Translation

    Target ORF information:

    RefSeq Version XM_022426573.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATACAGATGA GAGACATAGC TGAAGAGCTC
    TTATTACAGA AGCATGAGAG GAAAATCGGG GTTTGGAAGG ACTTGTGCCA TTCTCATTGT
    GAAGAGTCCA TCATCCATAA GAAGATCATG ACTATCATCA ACTGCTTTAT TAATTCTAGT
    ATTCCACCAG CTTTACAAAT TGACATTCCA GTAGAGCAAG CCCAAAAAAT TATTGAACAC
    AGGAAGGAGT TAGGACCATA TGTGTTTAGA GAGGCACAGA TGACAATTTT TGGGGTTCTG
    TTTAAATTTT GGCCTCAGTT TTGTGAGTTT AGGAAGAACT TAACTGATGA AAAAATTATG
    AGTGTTTTAG AGAGAAGACA AGAATATAAT AAGCAGAAAA AAAAATTGGC AGTCATAGAA
    GACGAAAATT TTGCAAAGGA TGGAATCAAA CAATATCCAA GTTCTTCAGG ATCTGCAATC
    AAAACAGCTG CTTTAGCCAC TGACCCAGTT TTAGGCCTAC AAGCATATGG CCGGCAGCCA
    ACCTGGTGCT ACTCCAAGTA CATAGAAGCC TTAGAGCAAG AAAGGATTCT TCTTAAAATT
    CAAGAGGAGC TGGAAAAAAA GTTATTTACA GCCACACCAT CTTTTACAAA CTTCAAGTCT
    ACTGCTTCCA CTATGTCTTT GAAAAAGACC GTATCTTCCC ACAACATCCA GAGCTGGATC
    ACAGTTGTGG TGCTAGCTGA TAATATCCCA CCACAAAGAT GGGACACCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292755
    Clone ID Related Accession (Same CDS sequence) XM_022426574.1
    Accession Version XM_022426574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    AAAATCTGCT ACCACAAATA TGTGGTAAAA TACGTGTGTT CCAAGATCTG TATATTTTTA
    AAGATTGGAA TATTCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282282.1
    CDS19..3579
    Translation

    Target ORF information:

    RefSeq Version XM_022426574.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    AAAATCTGCT ACCACAAATA TGTGGTAAAA TACGTGTGTT CCAAGATCTG TATATTTTTA
    AAGATTGGAA TATTCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292756
    Clone ID Related Accession (Same CDS sequence) XM_022426575.1
    Accession Version XM_022426575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATGCCC CCTATGAAGA TGAGGCCATT AACGTCAACT ACAATATTAT GTGTCTCAGT 
    CGTGAAGAAG GTATTGAGTG GATCAAAAAA GAAAGACTTC CAGCATTTCT GGAAAGTGAT
    TGTTACTTTG AATACAGATT AGCCAAATTG GTCTCACAAG TAAGATGGAG TGGATCCGGC
    ATGAACATCA CAGTCAATAC AGAATTTTCT CCCTGGGTCC TGAGAAAACC ACCCAGTCCA
    CCACCACCTG TTGTTGAAGA AGACAATGTT ATAATTATGA AAAAGTTCTA CATTTCTCTT
    GGCGAGGGCT CCTATACTCA AACAAAAGAT TGGTTTGCAT TAGCAAAACA GAGTTTGCAA
    ACAGTACCAA CATTTTCATT ACCATCTTGT GTACTCCACA GCAAACCTGA ACCACCGGTT
    GTTTCATCCA TTTCTGAAAG CTTTATCTTT GATGATAGAA TTCACCCAAG GACAAAAAGG
    GACTCATCTG AAACCATTAG ATTAACTTCT CATTTTGAAG AAGAAGAAGA TGGGTCTGTA
    TCTGTACAAG ACACTCCTTC TCAAGCCCTT CTAAGAATAT ACCTTGAGAG GCAACAGGAT
    GCTGATGAAA GCCTTCCATT GCATTTCACA ACATGTGAAG AATTTTTAAG ATCTTATATA
    GATTTTATTT TGAGAGTAGC AATTCATCAA ATTTTTGAAG AGCCACTTAC AGAAAGTCCA
    GATTATATAA ATTTCAACAA AGTAGCAGAA GTGGTGCTCG AGGAGGGTTT TGAGCCTCTC
    CATGGCAGGA ATGTTTTAAG TGAAACATTT CAAACCACAA ATGAAAAGTC AAAAGAGATG
    TTAGAACGAG CAAATTCAAA GAGTGAGAGC ATTGGACCAG AAAGCAGGGC TTATTGGTGT
    ATGTCTCACA AAGCTTATGA TATTGGCAAT AGAAAGGAGT TTGAAAGATT TAAGAAATTT
    ATAAAAGGTA CTTTAGGAGA GAGATATTGG TCATTATGGA TGGATATTGA GAGGTTAAAA
    GTTCTTAAGG ACCGCGGAAG ACATCAAAGA CATCTTGAGA AGATGAAAAA ATTCTATCTA
    GTGAGCAATG GAGATTGCTA TCTTTCTACA GAAATATTAT CAAAATTTAA ACTGCTAGAT
    GCATCTCAGT GGAATGAAGA ACACTTAAGA AATATTCAGA CAGAGGTTGT AAAACCATTA
    CTTCTATATT GGGCACCAAG ATTCTGTGTT ACTCATTCAT CCTCAGCTAA ACATGCTAGC
    GCTGAACTGA AATTCTGGCA TCTCCGTCAA GAGAAACCAA GGAAGGACGT TGACCCTTTT
    CCACAAATGG CAACCCTCTT GCCATTAAGA CCTAAATCTT GCATTCCACA GATATCTGAT
    ATGCAGAAAG AAGAATCCAA CTTATTACAA TGTCCTAAAT CTCCCAAGAA ACCACCTAGA
    GTAAAAACAG CAGGTCAAAA ACCTTGGAAG AGCAAGTCTT TGTATCTAAT TTCTTCGAAG
    GATGATGCAA TTGAGAAAGG GTTGAGGCAC AGGTCAGAAA GCAACAAAGT TATTCGCTTA
    ACATCTTTTA CTGACATTTC TGAATGTCTG AAGCCCCAGC TGGATAGAAG ATACACTTAC
    ACAGAAGAAC CTTCGGTTAA AACCATGACA GATGGTGCCT TGGGAGGATT TGACATGGAA
    AATTTGTTGC AATCTTTGTA TGTAGAAAAT AGAGCTGGAT TCTTCTTTAC TAAATTCTGT
    GAGCATTCAG GAAACAAGTT GTGGAAGAAC AGTGTGTACT TTTGGTTTGA CCTACAGGCT
    TATCATCAGC TTTTCTACCA AGAAACACTT CACCCTTTTA AAGTATGCAA ACAAGCCCAA
    TATCTTTTTG CCACATACAT TGCTCCTTCG GCTACTCTTG ACATCGGACT CCAACAGGAA
    AAGAAAAAAG AAATTTATAT GAAAATACAG CCACCATTTG AAGACCTTTT TGACACAGCA
    GAAGAATATA TCCTTCTCCT CCTCCTTGAG CCGTGGACAA AGATGGTAAA ATTAGACCAA
    GTTGCTTTTG GAAAGGTGGA GCTGGTGGAA GACACTCGAC AGCTAGACTC CACATACTTC
    AGAAAGCTAC AGGCTTTGCA TAAAGAGACC TTTTCCAAGA AAGCTGAGGA CACTACTTGT
    GAAATTGGAA CCGGTATTTC ACGGCTTCCT GAGGTCTCTA AAAAAACAGA ATACTGGAGT
    AATGTTCCTG AAGAATATAG GCACTTTAAT TTTAATGACC TGCTTAACAA CAAACTGGAG
    TTTGAACATT TCCGTCAGTT TCTTGAGGCT CATTCTTCAA GTTTGGACCT TATGTGCTGG
    ACAGACATTG AACAGTTCCG AAGAATCATT TATGGAGACC GAAAGCAGAG GGAAGCAAAA
    TCTATATATA TTAAAAACAA ATACCTTAAT AAAAAATATT TCTTTGGCCC CCAAAGTCCA
    GCTTCTCAAT ATCAGCAGGA CCAGATAATG AATTTAAGTG GTGGCTGGGG ACAGATGCTC
    CATGAGCAGC TTGATGCACC TGTTCTAATT GAAGTTCAGA AGCATGTTCT CAATAAGCTA
    GAAAATGTGT GGTTGCCATT GTTTCTTGCA AGCGAACAGT TTGTAGCACG TCAAAAGATA
    AAGATACAGA TGAGAGACAT AGCTGAAGAG CTCTTATTAC AGAAGCATGA GAGGAAAATC
    GGGGTTTGGA AGCCTGTGGA GAGTAAATGG ATATCTTCAT CTTGTGAAAT CATTGCTTTT
    CGTAAAGCAT TATTGAATCC AGTTACCGTA AGACAATTTC AACGGTTTGT GGCTCTAAAA
    GGAGATTTAT TGGAAAATGA TGTACTCTTT TGGCAAGAAG TACAAAAATA TAAGGACTTG
    TGCCATTCTC ATTGTGAAGA GTCCATCATC CATAAGAAGA TCATGACTAT CATCAACTGC
    TTTATTAATT CTAGTATTCC ACCAGCTTTA CAAATTGACA TTCCAGTAGA GCAAGCCCAA
    AAAATTATTG AACACAGGAA GGAGTTAGGA CCATATGTGT TTAGAGAGGC ACAGATGACA
    ATTTTTGGGG TTCTGTTTAA ATTTTGGCCT CAGTTTTGTG AGTTTAGGAA GAACTTAACT
    GATGAAAAAA TTATGAGTGT TTTAGAGAGA AGACAAGAAT ATAATAAGCA GAAAAAAAAA
    TTGGCAGTCA TAGAAGACGA AAATTTTGCA AAGGATGGAA TCAAACAATA TCCAAGTTCT
    TCAGGATCTG CAATCAAAAC AGCTGCTTTA GCCACTGACC CAGTTTTAGG CCTACAAGCA
    TATGGCCGGC AGCCAACCTG GTGCTACTCC AAGTACATAG AAGCCTTAGA GCAAGAAAGG
    ATTCTTCTTA AAATTCAAGA GGAGCTGGAA AAAAAGTTAT TTACAGCCAC ACCATCTTTT
    ACAAACTTCA AGTCTACTGC TTCCACTATG TCTTTGAAAA AGACCGTATC TTCCCACAAC
    ATCCAGAGCT GGATCACAGT TGTGGTGCTA GCTGATAATA TCCCACCACA AAGATGGGAC
    ACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282283.1
    CDS384..3932
    Translation

    Target ORF information:

    RefSeq Version XM_022426575.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATGCCC CCTATGAAGA TGAGGCCATT AACGTCAACT ACAATATTAT GTGTCTCAGT 
    CGTGAAGAAG GTATTGAGTG GATCAAAAAA GAAAGACTTC CAGCATTTCT GGAAAGTGAT
    TGTTACTTTG AATACAGATT AGCCAAATTG GTCTCACAAG TAAGATGGAG TGGATCCGGC
    ATGAACATCA CAGTCAATAC AGAATTTTCT CCCTGGGTCC TGAGAAAACC ACCCAGTCCA
    CCACCACCTG TTGTTGAAGA AGACAATGTT ATAATTATGA AAAAGTTCTA CATTTCTCTT
    GGCGAGGGCT CCTATACTCA AACAAAAGAT TGGTTTGCAT TAGCAAAACA GAGTTTGCAA
    ACAGTACCAA CATTTTCATT ACCATCTTGT GTACTCCACA GCAAACCTGA ACCACCGGTT
    GTTTCATCCA TTTCTGAAAG CTTTATCTTT GATGATAGAA TTCACCCAAG GACAAAAAGG
    GACTCATCTG AAACCATTAG ATTAACTTCT CATTTTGAAG AAGAAGAAGA TGGGTCTGTA
    TCTGTACAAG ACACTCCTTC TCAAGCCCTT CTAAGAATAT ACCTTGAGAG GCAACAGGAT
    GCTGATGAAA GCCTTCCATT GCATTTCACA ACATGTGAAG AATTTTTAAG ATCTTATATA
    GATTTTATTT TGAGAGTAGC AATTCATCAA ATTTTTGAAG AGCCACTTAC AGAAAGTCCA
    GATTATATAA ATTTCAACAA AGTAGCAGAA GTGGTGCTCG AGGAGGGTTT TGAGCCTCTC
    CATGGCAGGA ATGTTTTAAG TGAAACATTT CAAACCACAA ATGAAAAGTC AAAAGAGATG
    TTAGAACGAG CAAATTCAAA GAGTGAGAGC ATTGGACCAG AAAGCAGGGC TTATTGGTGT
    ATGTCTCACA AAGCTTATGA TATTGGCAAT AGAAAGGAGT TTGAAAGATT TAAGAAATTT
    ATAAAAGGTA CTTTAGGAGA GAGATATTGG TCATTATGGA TGGATATTGA GAGGTTAAAA
    GTTCTTAAGG ACCGCGGAAG ACATCAAAGA CATCTTGAGA AGATGAAAAA ATTCTATCTA
    GTGAGCAATG GAGATTGCTA TCTTTCTACA GAAATATTAT CAAAATTTAA ACTGCTAGAT
    GCATCTCAGT GGAATGAAGA ACACTTAAGA AATATTCAGA CAGAGGTTGT AAAACCATTA
    CTTCTATATT GGGCACCAAG ATTCTGTGTT ACTCATTCAT CCTCAGCTAA ACATGCTAGC
    GCTGAACTGA AATTCTGGCA TCTCCGTCAA GAGAAACCAA GGAAGGACGT TGACCCTTTT
    CCACAAATGG CAACCCTCTT GCCATTAAGA CCTAAATCTT GCATTCCACA GATATCTGAT
    ATGCAGAAAG AAGAATCCAA CTTATTACAA TGTCCTAAAT CTCCCAAGAA ACCACCTAGA
    GTAAAAACAG CAGGTCAAAA ACCTTGGAAG AGCAAGTCTT TGTATCTAAT TTCTTCGAAG
    GATGATGCAA TTGAGAAAGG GTTGAGGCAC AGGTCAGAAA GCAACAAAGT TATTCGCTTA
    ACATCTTTTA CTGACATTTC TGAATGTCTG AAGCCCCAGC TGGATAGAAG ATACACTTAC
    ACAGAAGAAC CTTCGGTTAA AACCATGACA GATGGTGCCT TGGGAGGATT TGACATGGAA
    AATTTGTTGC AATCTTTGTA TGTAGAAAAT AGAGCTGGAT TCTTCTTTAC TAAATTCTGT
    GAGCATTCAG GAAACAAGTT GTGGAAGAAC AGTGTGTACT TTTGGTTTGA CCTACAGGCT
    TATCATCAGC TTTTCTACCA AGAAACACTT CACCCTTTTA AAGTATGCAA ACAAGCCCAA
    TATCTTTTTG CCACATACAT TGCTCCTTCG GCTACTCTTG ACATCGGACT CCAACAGGAA
    AAGAAAAAAG AAATTTATAT GAAAATACAG CCACCATTTG AAGACCTTTT TGACACAGCA
    GAAGAATATA TCCTTCTCCT CCTCCTTGAG CCGTGGACAA AGATGGTAAA ATTAGACCAA
    GTTGCTTTTG GAAAGGTGGA GCTGGTGGAA GACACTCGAC AGCTAGACTC CACATACTTC
    AGAAAGCTAC AGGCTTTGCA TAAAGAGACC TTTTCCAAGA AAGCTGAGGA CACTACTTGT
    GAAATTGGAA CCGGTATTTC ACGGCTTCCT GAGGTCTCTA AAAAAACAGA ATACTGGAGT
    AATGTTCCTG AAGAATATAG GCACTTTAAT TTTAATGACC TGCTTAACAA CAAACTGGAG
    TTTGAACATT TCCGTCAGTT TCTTGAGGCT CATTCTTCAA GTTTGGACCT TATGTGCTGG
    ACAGACATTG AACAGTTCCG AAGAATCATT TATGGAGACC GAAAGCAGAG GGAAGCAAAA
    TCTATATATA TTAAAAACAA ATACCTTAAT AAAAAATATT TCTTTGGCCC CCAAAGTCCA
    GCTTCTCAAT ATCAGCAGGA CCAGATAATG AATTTAAGTG GTGGCTGGGG ACAGATGCTC
    CATGAGCAGC TTGATGCACC TGTTCTAATT GAAGTTCAGA AGCATGTTCT CAATAAGCTA
    GAAAATGTGT GGTTGCCATT GTTTCTTGCA AGCGAACAGT TTGTAGCACG TCAAAAGATA
    AAGATACAGA TGAGAGACAT AGCTGAAGAG CTCTTATTAC AGAAGCATGA GAGGAAAATC
    GGGGTTTGGA AGCCTGTGGA GAGTAAATGG ATATCTTCAT CTTGTGAAAT CATTGCTTTT
    CGTAAAGCAT TATTGAATCC AGTTACCGTA AGACAATTTC AACGGTTTGT GGCTCTAAAA
    GGAGATTTAT TGGAAAATGA TGTACTCTTT TGGCAAGAAG TACAAAAATA TAAGGACTTG
    TGCCATTCTC ATTGTGAAGA GTCCATCATC CATAAGAAGA TCATGACTAT CATCAACTGC
    TTTATTAATT CTAGTATTCC ACCAGCTTTA CAAATTGACA TTCCAGTAGA GCAAGCCCAA
    AAAATTATTG AACACAGGAA GGAGTTAGGA CCATATGTGT TTAGAGAGGC ACAGATGACA
    ATTTTTGGGG TTCTGTTTAA ATTTTGGCCT CAGTTTTGTG AGTTTAGGAA GAACTTAACT
    GATGAAAAAA TTATGAGTGT TTTAGAGAGA AGACAAGAAT ATAATAAGCA GAAAAAAAAA
    TTGGCAGTCA TAGAAGACGA AAATTTTGCA AAGGATGGAA TCAAACAATA TCCAAGTTCT
    TCAGGATCTG CAATCAAAAC AGCTGCTTTA GCCACTGACC CAGTTTTAGG CCTACAAGCA
    TATGGCCGGC AGCCAACCTG GTGCTACTCC AAGTACATAG AAGCCTTAGA GCAAGAAAGG
    ATTCTTCTTA AAATTCAAGA GGAGCTGGAA AAAAAGTTAT TTACAGCCAC ACCATCTTTT
    ACAAACTTCA AGTCTACTGC TTCCACTATG TCTTTGAAAA AGACCGTATC TTCCCACAAC
    ATCCAGAGCT GGATCACAGT TGTGGTGCTA GCTGATAATA TCCCACCACA AAGATGGGAC
    ACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292757
    Clone ID Related Accession (Same CDS sequence) XM_022426576.1
    Accession Version XM_022426576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGATACAG
    ATGAGAGACA TAGCTGAAGA GCTCTTATTA CAGAAGCATG AGAGGAAAAT CGGGGTTTGG
    AAGCCTGTGG AGAGTAAATG GATATCTTCA TCTTGTGAAA TCATTGCTTT TCGTAAAGCA
    TTATTGAATC CAGTTACCGT AAGACAATTT CAACGGTTTG TGGCTCTAAA AGGAGATTTA
    TTGGAAAATG ATGTACTCTT TTGGCAAGAA GTACAAAAAT ATAAGGACTT GTGCCATTCT
    CATTGTGAAG AGTCCATCAT CCATAAGAAG ATCATGACTA TCATCAACTG CTTTATTAAT
    TCTAGTATTC CACCAGCTTT ACAAATTGAC ATTCCAGTAG AGCAAGCCCA AAAAATTATT
    GAACACAGGA AGGAGTTAGG ACCATATGTG TTTAGAGAGG CACAGATGAC AATTTTTGGG
    GTTCTGTTTA AATTTTGGCC TCAGTTTTGT GAGTTTAGGA AGAACTTAAC TGATGAAAAA
    ATTATGAGTG TTTTAGAGAG AAGACAAGAA TATAATAAGC AGAAAAAAAA ATTGGCAGTC
    ATAGAAGACG AAAATTTTGC AAAGGATGGA ATCAAACAAT ATCCAAGTTC TTCAGGATCT
    GCAATCAAAA CAGCTGCTTT AGCCACTGAC CCAGTTTTAG GCCTACAAGC ATATGGCCGG
    CAGCCAACCT GGTGCTACTC CAAGTACATA GAAGCCTTAG AGCAAGAAAG GATTCTTCTT
    AAAATTCAAG AGGAGCTGGA AAAAAAGTTA TTTACAGCCA CACCATCTTT TACAAACTTC
    AAGTCTACTG CTTCCACTAT GTCTTTGAAA AAGACCGTAT CTTCCCACAA CATCCAGAGC
    TGGATCACAG TTGTGGTGCT AGCTGATAAT ATCCCACCAC AAAGATGGGA CACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282284.1
    CDS19..3498
    Translation

    Target ORF information:

    RefSeq Version XM_022426576.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGATACAG
    ATGAGAGACA TAGCTGAAGA GCTCTTATTA CAGAAGCATG AGAGGAAAAT CGGGGTTTGG
    AAGCCTGTGG AGAGTAAATG GATATCTTCA TCTTGTGAAA TCATTGCTTT TCGTAAAGCA
    TTATTGAATC CAGTTACCGT AAGACAATTT CAACGGTTTG TGGCTCTAAA AGGAGATTTA
    TTGGAAAATG ATGTACTCTT TTGGCAAGAA GTACAAAAAT ATAAGGACTT GTGCCATTCT
    CATTGTGAAG AGTCCATCAT CCATAAGAAG ATCATGACTA TCATCAACTG CTTTATTAAT
    TCTAGTATTC CACCAGCTTT ACAAATTGAC ATTCCAGTAG AGCAAGCCCA AAAAATTATT
    GAACACAGGA AGGAGTTAGG ACCATATGTG TTTAGAGAGG CACAGATGAC AATTTTTGGG
    GTTCTGTTTA AATTTTGGCC TCAGTTTTGT GAGTTTAGGA AGAACTTAAC TGATGAAAAA
    ATTATGAGTG TTTTAGAGAG AAGACAAGAA TATAATAAGC AGAAAAAAAA ATTGGCAGTC
    ATAGAAGACG AAAATTTTGC AAAGGATGGA ATCAAACAAT ATCCAAGTTC TTCAGGATCT
    GCAATCAAAA CAGCTGCTTT AGCCACTGAC CCAGTTTTAG GCCTACAAGC ATATGGCCGG
    CAGCCAACCT GGTGCTACTC CAAGTACATA GAAGCCTTAG AGCAAGAAAG GATTCTTCTT
    AAAATTCAAG AGGAGCTGGA AAAAAAGTTA TTTACAGCCA CACCATCTTT TACAAACTTC
    AAGTCTACTG CTTCCACTAT GTCTTTGAAA AAGACCGTAT CTTCCCACAA CATCCAGAGC
    TGGATCACAG TTGTGGTGCT AGCTGATAAT ATCCCACCAC AAAGATGGGA CACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292758
    Clone ID Related Accession (Same CDS sequence) XM_022426577.1
    Accession Version XM_022426577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGG ATGGAATCAA ACAATATCCA AGTTCTTCAG GATCTGCAAT CAAAACAGCT
    GCTTTAGCCA CTGACCCAGT TTTAGGCCTA CAAGCATATG GCCGGCAGCC AACCTGGTGC
    TACTCCAAGT ACATAGAAGC CTTAGAGCAA GAAAGGATTC TTCTTAAAAT TCAAGAGGAG
    CTGGAAAAAA AGTTATTTAC AGCCACACCA TCTTTTACAA ACTTCAAGTC TACTGCTTCC
    ACTATGTCTT TGAAAAAGAC CGTATCTTCC CACAACATCC AGAGCTGGAT CACAGTTGTG
    GTGCTAGCTG ATAATATCCC ACCACAAAGA TGGGACACCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282285.1
    CDS19..3423
    Translation

    Target ORF information:

    RefSeq Version XM_022426577.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGG ATGGAATCAA ACAATATCCA AGTTCTTCAG GATCTGCAAT CAAAACAGCT
    GCTTTAGCCA CTGACCCAGT TTTAGGCCTA CAAGCATATG GCCGGCAGCC AACCTGGTGC
    TACTCCAAGT ACATAGAAGC CTTAGAGCAA GAAAGGATTC TTCTTAAAAT TCAAGAGGAG
    CTGGAAAAAA AGTTATTTAC AGCCACACCA TCTTTTACAA ACTTCAAGTC TACTGCTTCC
    ACTATGTCTT TGAAAAAGAC CGTATCTTCC CACAACATCC AGAGCTGGAT CACAGTTGTG
    GTGCTAGCTG ATAATATCCC ACCACAAAGA TGGGACACCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292759
    Clone ID Related Accession (Same CDS sequence) XM_022426578.1
    Accession Version XM_022426578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGATACAG
    ATGAGAGACA TAGCTGAAGA GCTCTTATTA CAGAAGCATG AGAGGAAAAT CGGGGTTTGG
    AAGGACTTGT GCCATTCTCA TTGTGAAGAG TCCATCATCC ATAAGAAGAT CATGACTATC
    ATCAACTGCT TTATTAATTC TAGTATTCCA CCAGCTTTAC AAATTGACAT TCCAGTAGAG
    CAAGCCCAAA AAATTATTGA ACACAGGAAG GAGTTAGGAC CATATGTGTT TAGAGAGGCA
    CAGATGACAA TTTTTGGGGT TCTGTTTAAA TTTTGGCCTC AGTTTTGTGA GTTTAGGAAG
    AACTTAACTG ATGAAAAAAT TATGAGTGTT TTAGAGAGAA GACAAGAATA TAATAAGCAG
    AAAAAAAAAT TGGCAGTCAT AGAAGACGAA AATTTTGCAA AGGATGGAAT CAAACAATAT
    CCAAGTTCTT CAGGATCTGC AATCAAAACA GCTGCTTTAG CCACTGACCC AGTTTTAGGC
    CTACAAGCAT ATGGCCGGCA GCCAACCTGG TGCTACTCCA AGTACATAGA AGCCTTAGAG
    CAAGAAAGGA TTCTTCTTAA AATTCAAGAG GAGCTGGAAA AAAAGTTATT TACAGCCACA
    CCATCTTTTA CAAACTTCAA GTCTACTGCT TCCACTATGT CTTTGAAAAA GACCGTATCT
    TCCCACAACA TCCAGAGCTG GATCACAGTT GTGGTGCTAG CTGATAATAT CCCACCACAA
    AGATGGGACA CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282286.1
    CDS19..3336
    Translation

    Target ORF information:

    RefSeq Version XM_022426578.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGATACAG
    ATGAGAGACA TAGCTGAAGA GCTCTTATTA CAGAAGCATG AGAGGAAAAT CGGGGTTTGG
    AAGGACTTGT GCCATTCTCA TTGTGAAGAG TCCATCATCC ATAAGAAGAT CATGACTATC
    ATCAACTGCT TTATTAATTC TAGTATTCCA CCAGCTTTAC AAATTGACAT TCCAGTAGAG
    CAAGCCCAAA AAATTATTGA ACACAGGAAG GAGTTAGGAC CATATGTGTT TAGAGAGGCA
    CAGATGACAA TTTTTGGGGT TCTGTTTAAA TTTTGGCCTC AGTTTTGTGA GTTTAGGAAG
    AACTTAACTG ATGAAAAAAT TATGAGTGTT TTAGAGAGAA GACAAGAATA TAATAAGCAG
    AAAAAAAAAT TGGCAGTCAT AGAAGACGAA AATTTTGCAA AGGATGGAAT CAAACAATAT
    CCAAGTTCTT CAGGATCTGC AATCAAAACA GCTGCTTTAG CCACTGACCC AGTTTTAGGC
    CTACAAGCAT ATGGCCGGCA GCCAACCTGG TGCTACTCCA AGTACATAGA AGCCTTAGAG
    CAAGAAAGGA TTCTTCTTAA AATTCAAGAG GAGCTGGAAA AAAAGTTATT TACAGCCACA
    CCATCTTTTA CAAACTTCAA GTCTACTGCT TCCACTATGT CTTTGAAAAA GACCGTATCT
    TCCCACAACA TCCAGAGCTG GATCACAGTT GTGGTGCTAG CTGATAATAT CCCACCACAA
    AGATGGGACA CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292760
    Clone ID Related Accession (Same CDS sequence) XM_022426579.1
    Accession Version XM_022426579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGCTGG
    ATCACAGTTG TGGTGCTAGC TGATAATATC CCACCACAAA GATGGGACAC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282287.1
    CDS19..3315
    Translation

    Target ORF information:

    RefSeq Version XM_022426579.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGCTGG
    ATCACAGTTG TGGTGCTAGC TGATAATATC CCACCACAAA GATGGGACAC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292761
    Clone ID Related Accession (Same CDS sequence) XM_022426580.1
    Accession Version XM_022426580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACTTG
    TGCCATTCTC ATTGTGAAGA GTCCATCATC CATAAGAAGA TCATGACTAT CATCAACTGC
    TTTATTAATT CTAGTATTCC ACCAGCTTTA CAAATTGACA TTCCAGTAGA GCAAGCCCAA
    AAAATTATTG AACACAGGAA GGAGTTAGGA CCATATGTGT TTAGAGAGGC ACAGATGACA
    ATTTTTGGGG TTCTGTTTAA ATTTTGGCCT CAGTTTTGTG AGTTTAGGAA GAACTTAACT
    GATGAAAAAA TTATGAGTGT TTTAGAGAGA AGACAAGAAT ATAATAAGCA GAAAAAAAAA
    TTGGCAGTCA TAGAAGACGA AAATTTTGCA AAGGATGGAA TCAAACAATA TCCAAGTTCT
    TCAGGATCTG CAATCAAAAC AGCTGCTTTA GCCACTGACC CAGTTTTAGG CCTACAAGCA
    TATGGCCGGC AGCCAACCTG GTGCTACTCC AAGTACATAG AAGCCTTAGA GCAAGAAAGG
    ATTCTTCTTA AAATTCAAGA GGAGCTGGAA AAAAAGTTAT TTACAGCCAC ACCATCTTTT
    ACAAACTTCA AGTCTACTGC TTCCACTATG TCTTTGAAAA AGACCGTATC TTCCCACAAC
    ATCCAGAGCT GGATCACAGT TGTGGTGCTA GCTGATAATA TCCCACCACA AAGATGGGAC
    ACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282288.1
    CDS19..3267
    Translation

    Target ORF information:

    RefSeq Version XM_022426580.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGGCTTC 
    CGGATATATC TGTATGTCTC CAGTCCAGAA CTCTGTATTC GTTGGGTTAT CGGGCACCTT
    CATCATCTGG ATGCCCCTGC ATCACCTCCA CAATCACATT CCACCCTCTG TTTTTTTCAA
    GAGTGTGATC AGGAAGATTC TTTGGCAACA GATGATTTCC TTGTGGACTA CTTTAATGAA
    TTCCTAAGCC TTCCAACCTT TTCAGAGGCA GTTAGATTTA ATGTGGATTA TGGAGTTTTT
    GAAATAGTTA ACAATGCTCC ACAACTTCTA GAAAAGCAAC TGAAAAAAAT TCTTCGAAAC
    CAGCAACCTC GAAATCCCAT CTATGATGTT GTAAGGAAAG GAAAGAATGA TATTAAACCT
    GTTCAGATGA ATGCCCCCTA TGAAGATGAG GCCATTAACG TCAACTACAA TATTATGTGT
    CTCAGTCGTG AAGAAGGTAT TGAGTGGATC AAAAAAGAAA GACTTCCAGC ATTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGATTAGCC AAATTGGTCT CACAAGTAAG ATGGAGTGGA
    TCCGGCATGA ACATCACAGT CAATACAGAA TTTTCTCCCT GGGTCCTGAG AAAACCACCC
    AGTCCACCAC CACCTGTTGT TGAAGAAGAC AATGTTATAA TTATGAAAAA GTTCTACATT
    TCTCTTGGCG AGGGCTCCTA TACTCAAACA AAAGATTGGT TTGCATTAGC AAAACAGAGT
    TTGCAAACAG TACCAACATT TTCATTACCA TCTTGTGTAC TCCACAGCAA ACCTGAACCA
    CCGGTTGTTT CATCCATTTC TGAAAGCTTT ATCTTTGATG ATAGAATTCA CCCAAGGACA
    AAAAGGGACT CATCTGAAAC CATTAGATTA ACTTCTCATT TTGAAGAAGA AGAAGATGGG
    TCTGTATCTG TACAAGACAC TCCTTCTCAA GCCCTTCTAA GAATATACCT TGAGAGGCAA
    CAGGATGCTG ATGAAAGCCT TCCATTGCAT TTCACAACAT GTGAAGAATT TTTAAGATCT
    TATATAGATT TTATTTTGAG AGTAGCAATT CATCAAATTT TTGAAGAGCC ACTTACAGAA
    AGTCCAGATT ATATAAATTT CAACAAAGTA GCAGAAGTGG TGCTCGAGGA GGGTTTTGAG
    CCTCTCCATG GCAGGAATGT TTTAAGTGAA ACATTTCAAA CCACAAATGA AAAGTCAAAA
    GAGATGTTAG AACGAGCAAA TTCAAAGAGT GAGAGCATTG GACCAGAAAG CAGGGCTTAT
    TGGTGTATGT CTCACAAAGC TTATGATATT GGCAATAGAA AGGAGTTTGA AAGATTTAAG
    AAATTTATAA AAGGTACTTT AGGAGAGAGA TATTGGTCAT TATGGATGGA TATTGAGAGG
    TTAAAAGTTC TTAAGGACCG CGGAAGACAT CAAAGACATC TTGAGAAGAT GAAAAAATTC
    TATCTAGTGA GCAATGGAGA TTGCTATCTT TCTACAGAAA TATTATCAAA ATTTAAACTG
    CTAGATGCAT CTCAGTGGAA TGAAGAACAC TTAAGAAATA TTCAGACAGA GGTTGTAAAA
    CCATTACTTC TATATTGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACTTG
    TGCCATTCTC ATTGTGAAGA GTCCATCATC CATAAGAAGA TCATGACTAT CATCAACTGC
    TTTATTAATT CTAGTATTCC ACCAGCTTTA CAAATTGACA TTCCAGTAGA GCAAGCCCAA
    AAAATTATTG AACACAGGAA GGAGTTAGGA CCATATGTGT TTAGAGAGGC ACAGATGACA
    ATTTTTGGGG TTCTGTTTAA ATTTTGGCCT CAGTTTTGTG AGTTTAGGAA GAACTTAACT
    GATGAAAAAA TTATGAGTGT TTTAGAGAGA AGACAAGAAT ATAATAAGCA GAAAAAAAAA
    TTGGCAGTCA TAGAAGACGA AAATTTTGCA AAGGATGGAA TCAAACAATA TCCAAGTTCT
    TCAGGATCTG CAATCAAAAC AGCTGCTTTA GCCACTGACC CAGTTTTAGG CCTACAAGCA
    TATGGCCGGC AGCCAACCTG GTGCTACTCC AAGTACATAG AAGCCTTAGA GCAAGAAAGG
    ATTCTTCTTA AAATTCAAGA GGAGCTGGAA AAAAAGTTAT TTACAGCCAC ACCATCTTTT
    ACAAACTTCA AGTCTACTGC TTCCACTATG TCTTTGAAAA AGACCGTATC TTCCCACAAC
    ATCCAGAGCT GGATCACAGT TGTGGTGCTA GCTGATAATA TCCCACCACA AAGATGGGAC
    ACCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39625
    Clone ID Related Accession (Same CDS sequence) XM_014118536.1
    Accession Version XM_014118536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGACTTCC
    TGCATTGAAG AAAGGAAGAT GGGCCCTTTG GAGACTTTTG CCAGATTTCT TTCACCAGCA
    ATTTCAAGAG CAAACACTTG GAATATCCTT GGTTACCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974011.1
    CDS193..4074
    Translation

    Target ORF information:

    RefSeq Version XM_014118536.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGACTTCC
    TGCATTGAAG AAAGGAAGAT GGGCCCTTTG GAGACTTTTG CCAGATTTCT TTCACCAGCA
    ATTTCAAGAG CAAACACTTG GAATATCCTT GGTTACCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39626
    Clone ID Related Accession (Same CDS sequence) XM_014118537.2 , XM_014118537.1
    Accession Version XM_014118537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 22 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGCTGG
    ATCACAGTTG TGGTGCTAGC TGATAATATC CCACCACAAA GATGGGACAC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974012.1
    CDS29..3865
    Translation

    Target ORF information:

    RefSeq Version XM_014118537.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 22 (RGS22), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGCTGG
    ATCACAGTTG TGGTGCTAGC TGATAATATC CCACCACAAA GATGGGACAC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39626
    Clone ID Related Accession (Same CDS sequence) XM_014118537.2 , XM_014118537.1
    Accession Version XM_014118537.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGCTGG
    ATCACAGTTG TGGTGCTAGC TGATAATATC CCACCACAAA GATGGGACAC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974012.1
    CDS193..4029
    Translation

    Target ORF information:

    RefSeq Version XM_014118537.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGCTGG
    ATCACAGTTG TGGTGCTAGC TGATAATATC CCACCACAAA GATGGGACAC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39627
    Clone ID Related Accession (Same CDS sequence) XM_014118538.1
    Accession Version XM_014118538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GCCTGTGGAG
    AGTAAATGGA TATCTTCATC TTGTGAAATC ATTGCTTTTC GTAAAGCATT ATTGAATCCA
    GTTACCGTAA GACAATTTCA ACGGTTTGTG GCTCTAAAAG GAGATTTATT GGAAAATGAT
    GTACTCTTTT GGCAAGAAGT ACAAAAATAT AAGGACTTGT GCCATTCTCA TTGTGAAGAG
    TCCATCATCC ATAAGAAGAT CATGACTATC ATCAACTGCT TTATTAATTC TAGTATTCCA
    CCAGCTTTAC AAATTGACAT TCCAGTAGAG CAAGCCCAAA AAATTATTGA ACACAGGAAG
    GAGTTAGGAC CATATGTGTT TAGAGAGGCA CAGATGACAA TTTTTGGGGT TCTGTTTAAA
    TTTTGGCCTC AGTTTTGTGA GTTTAGGAAG AACTTAACTG ATGAAAAAAT TATGAGTGTT
    TTAGAGAGAA GACAAGAATA TAATAAGCAG AAAAAAAAAT TGGCAGTCAT AGAAGACGAA
    AATTTTGCAA AGGATGGAAT CAAACAATAT CCAAGTTCTT CAGGATCTGC AATCAAAACA
    GCTGCTTTAG CCACTGACCC AGTTTTAGGC CTACAAGCAT ATGGCCGGCA GCCAACCTGG
    TGCTACTCCA AGTACATAGA AGCCTTAGAG CAAGAAAGGA TTCTTCTTAA AATTCAAGAG
    GAGCTGGAAA AAAAGTTATT TACAGCCACA CCATCTTTTA CAAACTTCAA GTCTACTGCT
    TCCACTATGT CTTTGAAAAA GACCGTATCT TCCCACAACA TCCAGACTTC CTGCATTGAA
    GAAAGGAAGA TGGGCCCTTT GGAGACTTTT GCCAGATTTC TTTCACCAGC AATTTCAAGA
    GCAAACACTT GGAATATCCT TGGTTACCAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974013.1
    CDS193..4005
    Translation

    Target ORF information:

    RefSeq Version XM_014118538.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GCCTGTGGAG
    AGTAAATGGA TATCTTCATC TTGTGAAATC ATTGCTTTTC GTAAAGCATT ATTGAATCCA
    GTTACCGTAA GACAATTTCA ACGGTTTGTG GCTCTAAAAG GAGATTTATT GGAAAATGAT
    GTACTCTTTT GGCAAGAAGT ACAAAAATAT AAGGACTTGT GCCATTCTCA TTGTGAAGAG
    TCCATCATCC ATAAGAAGAT CATGACTATC ATCAACTGCT TTATTAATTC TAGTATTCCA
    CCAGCTTTAC AAATTGACAT TCCAGTAGAG CAAGCCCAAA AAATTATTGA ACACAGGAAG
    GAGTTAGGAC CATATGTGTT TAGAGAGGCA CAGATGACAA TTTTTGGGGT TCTGTTTAAA
    TTTTGGCCTC AGTTTTGTGA GTTTAGGAAG AACTTAACTG ATGAAAAAAT TATGAGTGTT
    TTAGAGAGAA GACAAGAATA TAATAAGCAG AAAAAAAAAT TGGCAGTCAT AGAAGACGAA
    AATTTTGCAA AGGATGGAAT CAAACAATAT CCAAGTTCTT CAGGATCTGC AATCAAAACA
    GCTGCTTTAG CCACTGACCC AGTTTTAGGC CTACAAGCAT ATGGCCGGCA GCCAACCTGG
    TGCTACTCCA AGTACATAGA AGCCTTAGAG CAAGAAAGGA TTCTTCTTAA AATTCAAGAG
    GAGCTGGAAA AAAAGTTATT TACAGCCACA CCATCTTTTA CAAACTTCAA GTCTACTGCT
    TCCACTATGT CTTTGAAAAA GACCGTATCT TCCCACAACA TCCAGACTTC CTGCATTGAA
    GAAAGGAAGA TGGGCCCTTT GGAGACTTTT GCCAGATTTC TTTCACCAGC AATTTCAAGA
    GCAAACACTT GGAATATCCT TGGTTACCAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39628
    Clone ID Related Accession (Same CDS sequence) XM_014118539.1
    Accession Version XM_014118539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGAATT
    TCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974014.1
    CDS193..3978
    Translation

    Target ORF information:

    RefSeq Version XM_014118539.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGATGACAAT TTTTGGGGTT
    CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG TTTAGGAAGA ACTTAACTGA TGAAAAAATT
    ATGAGTGTTT TAGAGAGAAG ACAAGAATAT AATAAGCAGA AAAAAAAATT GGCAGTCATA
    GAAGACGAAA ATTTTGCAAA GGATGGAATC AAACAATATC CAAGTTCTTC AGGATCTGCA
    ATCAAAACAG CTGCTTTAGC CACTGACCCA GTTTTAGGCC TACAAGCATA TGGCCGGCAG
    CCAACCTGGT GCTACTCCAA GTACATAGAA GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA
    ATTCAAGAGG AGCTGGAAAA AAAGTTATTT ACAGCCACAC CATCTTTTAC AAACTTCAAG
    TCTACTGCTT CCACTATGTC TTTGAAAAAG ACCGTATCTT CCCACAACAT CCAGAGAATT
    TCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39629
    Clone ID Related Accession (Same CDS sequence) XM_014118540.1
    Accession Version XM_014118540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGGATGGAAT CAAACAATAT
    CCAAGTTCTT CAGGATCTGC AATCAAAACA GCTGCTTTAG CCACTGACCC AGTTTTAGGC
    CTACAAGCAT ATGGCCGGCA GCCAACCTGG TGCTACTCCA AGTACATAGA AGCCTTAGAG
    CAAGAAAGGA TTCTTCTTAA AATTCAAGAG GAGCTGGAAA AAAAGTTATT TACAGCCACA
    CCATCTTTTA CAAACTTCAA GTCTACTGCT TCCACTATGT CTTTGAAAAA GACCGTATCT
    TCCCACAACA TCCAGACTTC CTGCATTGAA GAAAGGAAGA TGGGCCCTTT GGAGACTTTT
    GCCAGATTTC TTTCACCAGC AATTTCAAGA GCAAACACTT GGAATATCCT TGGTTACCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974015.1
    CDS193..3915
    Translation

    Target ORF information:

    RefSeq Version XM_014118540.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGGATGGAAT CAAACAATAT
    CCAAGTTCTT CAGGATCTGC AATCAAAACA GCTGCTTTAG CCACTGACCC AGTTTTAGGC
    CTACAAGCAT ATGGCCGGCA GCCAACCTGG TGCTACTCCA AGTACATAGA AGCCTTAGAG
    CAAGAAAGGA TTCTTCTTAA AATTCAAGAG GAGCTGGAAA AAAAGTTATT TACAGCCACA
    CCATCTTTTA CAAACTTCAA GTCTACTGCT TCCACTATGT CTTTGAAAAA GACCGTATCT
    TCCCACAACA TCCAGACTTC CTGCATTGAA GAAAGGAAGA TGGGCCCTTT GGAGACTTTT
    GCCAGATTTC TTTCACCAGC AATTTCAAGA GCAAACACTT GGAATATCCT TGGTTACCAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39630
    Clone ID Related Accession (Same CDS sequence) XM_014118541.1
    Accession Version XM_014118541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGACTTCCTG CATTGAAGAA AGGAAGATGG GCCCTTTGGA GACTTTTGCC
    AGATTTCTTT CACCAGCAAT TTCAAGAGCA AACACTTGGA ATATCCTTGG TTACCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974016.1
    CDS193..3912
    Translation

    Target ORF information:

    RefSeq Version XM_014118541.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGGGC ACCAAGATTC TGTGTTACTC ATTCATCCTC AGCTAAACAT
    GCTAGCGCTG AACTGAAATT CTGGCATCTC CGTCAAGAGA AACCAAGGAA GGACGTTGAC
    CCTTTTCCAC AAATGGCAAC CCTCTTGCCA TTAAGACCTA AATCTTGCAT TCCACAGATA
    TCTGATATGC AGAAAGAAGA ATCCAACTTA TTACAATGTC CTAAATCTCC CAAGAAACCA
    CCTAGAGTAA AAACAGCAGG TCAAAAACCT TGGAAGAGCA AGTCTTTGTA TCTAATTTCT
    TCGAAGGATG ATGCAATTGA GAAAGGGTTG AGGCACAGGT CAGAAAGCAA CAAAGTTATT
    CGCTTAACAT CTTTTACTGA CATTTCTGAA TGTCTGAAGC CCCAGCTGGA TAGAAGATAC
    ACTTACACAG AAGAACCTTC GGTTAAAACC ATGACAGATG GTGCCTTGGG AGGATTTGAC
    ATGGAAAATT TGTTGCAATC TTTGTATGTA GAAAATAGAG CTGGATTCTT CTTTACTAAA
    TTCTGTGAGC ATTCAGGAAA CAAGTTGTGG AAGAACAGTG TGTACTTTTG GTTTGACCTA
    CAGGCTTATC ATCAGCTTTT CTACCAAGAA ACACTTCACC CTTTTAAAGT ATGCAAACAA
    GCCCAATATC TTTTTGCCAC ATACATTGCT CCTTCGGCTA CTCTTGACAT CGGACTCCAA
    CAGGAAAAGA AAAAAGAAAT TTATATGAAA ATACAGCCAC CATTTGAAGA CCTTTTTGAC
    ACAGCAGAAG AATATATCCT TCTCCTCCTC CTTGAGCCGT GGACAAAGAT GGTAAAATTA
    GACCAAGTTG CTTTTGGAAA GGTGGAGCTG GTGGAAGACA CTCGACAGCT AGACTCCACA
    TACTTCAGAA AGCTACAGGC TTTGCATAAA GAGACCTTTT CCAAGAAAGC TGAGGACACT
    ACTTGTGAAA TTGGAACCGG TATTTCACGG CTTCCTGAGG TCTCTAAAAA AACAGAATAC
    TGGAGTAATG TTCCTGAAGA ATATAGGCAC TTTAATTTTA ATGACCTGCT TAACAACAAA
    CTGGAGTTTG AACATTTCCG TCAGTTTCTT GAGGCTCATT CTTCAAGTTT GGACCTTATG
    TGCTGGACAG ACATTGAACA GTTCCGAAGA ATCATTTATG GAGACCGAAA GCAGAGGGAA
    GCAAAATCTA TATATATTAA AAACAAATAC CTTAATAAAA AATATTTCTT TGGCCCCCAA
    AGTCCAGCTT CTCAATATCA GCAGGACCAG ATAATGAATT TAAGTGGTGG CTGGGGACAG
    ATGCTCCATG AGCAGCTTGA TGCACCTGTT CTAATTGAAG TTCAGAAGCA TGTTCTCAAT
    AAGCTAGAAA ATGTGTGGTT GCCATTGTTT CTTGCAAGCG AACAGTTTGT AGCACGTCAA
    AAGATAAAGA TACAGATGAG AGACATAGCT GAAGAGCTCT TATTACAGAA GCATGAGAGG
    AAAATCGGGG TTTGGAAGCC TGTGGAGAGT AAATGGATAT CTTCATCTTG TGAAATCATT
    GCTTTTCGTA AAGCATTATT GAATCCAGTT ACCGTAAGAC AATTTCAACG GTTTGTGGCT
    CTAAAAGGAG ATTTATTGGA AAATGATGTA CTCTTTTGGC AAGAAGTACA AAAATATAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGACTTCCTG CATTGAAGAA AGGAAGATGG GCCCTTTGGA GACTTTTGCC
    AGATTTCTTT CACCAGCAAT TTCAAGAGCA AACACTTGGA ATATCCTTGG TTACCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39631
    Clone ID Related Accession (Same CDS sequence) XM_014118542.1
    Accession Version XM_014118542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGACTTCCTG CATTGAAGAA AGGAAGATGG GCCCTTTGGA GACTTTTGCC
    AGATTTCTTT CACCAGCAAT TTCAAGAGCA AACACTTGGA ATATCCTTGG TTACCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974017.1
    CDS193..3912
    Translation

    Target ORF information:

    RefSeq Version XM_014118542.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    GACTTGTGCC ATTCTCATTG TGAAGAGTCC ATCATCCATA AGAAGATCAT GACTATCATC
    AACTGCTTTA TTAATTCTAG TATTCCACCA GCTTTACAAA TTGACATTCC AGTAGAGCAA
    GCCCAAAAAA TTATTGAACA CAGGAAGGAG TTAGGACCAT ATGTGTTTAG AGAGGCACAG
    ATGACAATTT TTGGGGTTCT GTTTAAATTT TGGCCTCAGT TTTGTGAGTT TAGGAAGAAC
    TTAACTGATG AAAAAATTAT GAGTGTTTTA GAGAGAAGAC AAGAATATAA TAAGCAGAAA
    AAAAAATTGG CAGTCATAGA AGACGAAAAT TTTGCAAAGG ATGGAATCAA ACAATATCCA
    AGTTCTTCAG GATCTGCAAT CAAAACAGCT GCTTTAGCCA CTGACCCAGT TTTAGGCCTA
    CAAGCATATG GCCGGCAGCC AACCTGGTGC TACTCCAAGT ACATAGAAGC CTTAGAGCAA
    GAAAGGATTC TTCTTAAAAT TCAAGAGGAG CTGGAAAAAA AGTTATTTAC AGCCACACCA
    TCTTTTACAA ACTTCAAGTC TACTGCTTCC ACTATGTCTT TGAAAAAGAC CGTATCTTCC
    CACAACATCC AGACTTCCTG CATTGAAGAA AGGAAGATGG GCCCTTTGGA GACTTTTGCC
    AGATTTCTTT CACCAGCAAT TTCAAGAGCA AACACTTGGA ATATCCTTGG TTACCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39632
    Clone ID Related Accession (Same CDS sequence) XM_014118543.1
    Accession Version XM_014118543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GGACTTGTGC
    CATTCTCATT GTGAAGAGTC CATCATCCAT AAGAAGATCA TGACTATCAT CAACTGCTTT
    ATTAATTCTA GTATTCCACC AGCTTTACAA ATTGACATTC CAGTAGAGCA AGCCCAAAAA
    ATTATTGAAC ACAGGAAGGA GTTAGGACCA TATGTGTTTA GAGAGGCACA GATGACAATT
    TTTGGGGTTC TGTTTAAATT TTGGCCTCAG TTTTGTGAGT TTAGGAAGAA CTTAACTGAT
    GAAAAAATTA TGAGTGTTTT AGAGAGAAGA CAAGAATATA ATAAGCAGAA AAAAAAATTG
    GCAGTCATAG AAGACGAAAA TTTTGCAAAG GATGGAATCA AACAATATCC AAGTTCTTCA
    GGATCTGCAA TCAAAACAGC TGCTTTAGCC ACTGACCCAG TTTTAGGCCT ACAAGCATAT
    GGCCGGCAGC CAACCTGGTG CTACTCCAAG TACATAGAAG CCTTAGAGCA AGAAAGGATT
    CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA AAGTTATTTA CAGCCACACC ATCTTTTACA
    AACTTCAAGT CTACTGCTTC CACTATGTCT TTGAAAAAGA CCGTATCTTC CCACAACATC
    CAGACTTCCT GCATTGAAGA AAGGAAGATG GGCCCTTTGG AGACTTTTGC CAGATTTCTT
    TCACCAGCAA TTTCAAGAGC AAACACTTGG AATATCCTTG GTTACCACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974018.1
    CDS193..3843
    Translation

    Target ORF information:

    RefSeq Version XM_014118543.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GGACTTGTGC
    CATTCTCATT GTGAAGAGTC CATCATCCAT AAGAAGATCA TGACTATCAT CAACTGCTTT
    ATTAATTCTA GTATTCCACC AGCTTTACAA ATTGACATTC CAGTAGAGCA AGCCCAAAAA
    ATTATTGAAC ACAGGAAGGA GTTAGGACCA TATGTGTTTA GAGAGGCACA GATGACAATT
    TTTGGGGTTC TGTTTAAATT TTGGCCTCAG TTTTGTGAGT TTAGGAAGAA CTTAACTGAT
    GAAAAAATTA TGAGTGTTTT AGAGAGAAGA CAAGAATATA ATAAGCAGAA AAAAAAATTG
    GCAGTCATAG AAGACGAAAA TTTTGCAAAG GATGGAATCA AACAATATCC AAGTTCTTCA
    GGATCTGCAA TCAAAACAGC TGCTTTAGCC ACTGACCCAG TTTTAGGCCT ACAAGCATAT
    GGCCGGCAGC CAACCTGGTG CTACTCCAAG TACATAGAAG CCTTAGAGCA AGAAAGGATT
    CTTCTTAAAA TTCAAGAGGA GCTGGAAAAA AAGTTATTTA CAGCCACACC ATCTTTTACA
    AACTTCAAGT CTACTGCTTC CACTATGTCT TTGAAAAAGA CCGTATCTTC CCACAACATC
    CAGACTTCCT GCATTGAAGA AAGGAAGATG GGCCCTTTGG AGACTTTTGC CAGATTTCTT
    TCACCAGCAA TTTCAAGAGC AAACACTTGG AATATCCTTG GTTACCACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39633
    Clone ID Related Accession (Same CDS sequence) XM_014118544.1
    Accession Version XM_014118544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGGACTTGTG CCATTCTCAT TGTGAAGAGT CCATCATCCA TAAGAAGATC
    ATGACTATCA TCAACTGCTT TATTAATTCT AGTATTCCAC CAGCTTTACA AATTGACATT
    CCAGTAGAGC AAGCCCAAAA AATTATTGAA CACAGGAAGG AGTTAGGACC ATATGTGTTT
    AGAGAGGCAC AGATGACAAT TTTTGGGGTT CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG
    TTTAGGAAGA ACTTAACTGA TGAAAAAATT ATGAGTGTTT TAGAGAGAAG ACAAGAATAT
    AATAAGCAGA AAAAAAAATT GGCAGTCATA GAAGACGAAA ATTTTGCAAA GGATGGAATC
    AAACAATATC CAAGTTCTTC AGGATCTGCA ATCAAAACAG CTGCTTTAGC CACTGACCCA
    GTTTTAGGCC TACAAGCATA TGGCCGGCAG CCAACCTGGT GCTACTCCAA GTACATAGAA
    GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA ATTCAAGAGG AGCTGGAAAA AAAGTTATTT
    ACAGCCACAC CATCTTTTAC AAACTTCAAG TCTACTGCTT CCACTATGTC TTTGAAAAAG
    ACCGTATCTT CCCACAACAT CCAGACTTCC TGCATTGAAG AAAGGAAGAT GGGCCCTTTG
    GAGACTTTTG CCAGATTTCT TTCACCAGCA ATTTCAAGAG CAAACACTTG GAATATCCTT
    GGTTACCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974019.1
    CDS193..3684
    Translation

    Target ORF information:

    RefSeq Version XM_014118544.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGGACTTGTG CCATTCTCAT TGTGAAGAGT CCATCATCCA TAAGAAGATC
    ATGACTATCA TCAACTGCTT TATTAATTCT AGTATTCCAC CAGCTTTACA AATTGACATT
    CCAGTAGAGC AAGCCCAAAA AATTATTGAA CACAGGAAGG AGTTAGGACC ATATGTGTTT
    AGAGAGGCAC AGATGACAAT TTTTGGGGTT CTGTTTAAAT TTTGGCCTCA GTTTTGTGAG
    TTTAGGAAGA ACTTAACTGA TGAAAAAATT ATGAGTGTTT TAGAGAGAAG ACAAGAATAT
    AATAAGCAGA AAAAAAAATT GGCAGTCATA GAAGACGAAA ATTTTGCAAA GGATGGAATC
    AAACAATATC CAAGTTCTTC AGGATCTGCA ATCAAAACAG CTGCTTTAGC CACTGACCCA
    GTTTTAGGCC TACAAGCATA TGGCCGGCAG CCAACCTGGT GCTACTCCAA GTACATAGAA
    GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA ATTCAAGAGG AGCTGGAAAA AAAGTTATTT
    ACAGCCACAC CATCTTTTAC AAACTTCAAG TCTACTGCTT CCACTATGTC TTTGAAAAAG
    ACCGTATCTT CCCACAACAT CCAGACTTCC TGCATTGAAG AAAGGAAGAT GGGCCCTTTG
    GAGACTTTTG CCAGATTTCT TTCACCAGCA ATTTCAAGAG CAAACACTTG GAATATCCTT
    GGTTACCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39634
    Clone ID Related Accession (Same CDS sequence) XM_014118545.1
    Accession Version XM_014118545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGAAAATCTG CTACCACAAA
    TATGTGGTAA AATACGTGTG TTCCAAGATC TGTATATTTT TAAAGATTGG AATATTCACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974020.1
    CDS193..3615
    Translation

    Target ORF information:

    RefSeq Version XM_014118545.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GATACAGATG
    AGAGACATAG CTGAAGAGCT CTTATTACAG AAGCATGAGA GGAAAATCGG GGTTTGGAAG
    CCTGTGGAGA GTAAATGGAT ATCTTCATCT TGTGAAATCA TTGCTTTTCG TAAAGCATTA
    TTGAATCCAG TTACCGTAAG ACAATTTCAA CGGTTTGTGG CTCTAAAAGG AGATTTATTG
    GAAAATGATG TACTCTTTTG GCAAGAAGTA CAAAAATATA AGGACTTGTG CCATTCTCAT
    TGTGAAGAGT CCATCATCCA TAAGAAGATC ATGACTATCA TCAACTGCTT TATTAATTCT
    AGTATTCCAC CAGCTTTACA AATTGACATT CCAGTAGAGC AAGCCCAAAA AATTATTGAA
    CACAGGAAGG AGTTAGGACC ATATGTGTTT AGAGAGGCAC AGAAAATCTG CTACCACAAA
    TATGTGGTAA AATACGTGTG TTCCAAGATC TGTATATTTT TAAAGATTGG AATATTCACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39635
    Clone ID Related Accession (Same CDS sequence) XM_014118546.1
    Accession Version XM_014118546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGATAC AGATGAGAGA CATAGCTGAA
    GAGCTCTTAT TACAGAAGCA TGAGAGGAAA ATCGGGGTTT GGAAGCCTGT GGAGAGTAAA
    TGGATATCTT CATCTTGTGA AATCATTGCT TTTCGTAAAG CATTATTGAA TCCAGTTACC
    GTAAGACAAT TTCAACGGTT TGTGGCTCTA AAAGGAGATT TATTGGAAAA TGATGTACTC
    TTTTGGCAAG AAGTACAAAA ATATAAGGAC TTGTGCCATT CTCATTGTGA AGAGTCCATC
    ATCCATAAGA AGATCATGAC TATCATCAAC TGCTTTATTA ATTCTAGTAT TCCACCAGCT
    TTACAAATTG ACATTCCAGT AGAGCAAGCC CAAAAAATTA TTGAACACAG GAAGGAGTTA
    GGACCATATG TGTTTAGAGA GGCACAGATG ACAATTTTTG GGGTTCTGTT TAAATTTTGG
    CCTCAGTTTT GTGAGTTTAG GAAGAACTTA ACTGATGAAA AAATTATGAG TGTTTTAGAG
    AGAAGACAAG AATATAATAA GCAGAAAAAA AAATTGGCAG TCATAGAAGA CGAAAATTTT
    GCAAAGGATG GAATCAAACA ATATCCAAGT TCTTCAGGAT CTGCAATCAA AACAGCTGCT
    TTAGCCACTG ACCCAGTTTT AGGCCTACAA GCATATGGCC GGCAGCCAAC CTGGTGCTAC
    TCCAAGTACA TAGAAGCCTT AGAGCAAGAA AGGATTCTTC TTAAAATTCA AGAGGAGCTG
    GAAAAAAAGT TATTTACAGC CACACCATCT TTTACAAACT TCAAGTCTAC TGCTTCCACT
    ATGTCTTTGA AAAAGACCGT ATCTTCCCAC AACATCCAGA CTTCCTGCAT TGAAGAAAGG
    AAGATGGGCC CTTTGGAGAC TTTTGCCAGA TTTCTTTCAC CAGCAATTTC AAGAGCAAAC
    ACTTGGAATA TCCTTGGTTA CCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974021.1
    CDS193..3579
    Translation

    Target ORF information:

    RefSeq Version XM_014118546.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGATAC AGATGAGAGA CATAGCTGAA
    GAGCTCTTAT TACAGAAGCA TGAGAGGAAA ATCGGGGTTT GGAAGCCTGT GGAGAGTAAA
    TGGATATCTT CATCTTGTGA AATCATTGCT TTTCGTAAAG CATTATTGAA TCCAGTTACC
    GTAAGACAAT TTCAACGGTT TGTGGCTCTA AAAGGAGATT TATTGGAAAA TGATGTACTC
    TTTTGGCAAG AAGTACAAAA ATATAAGGAC TTGTGCCATT CTCATTGTGA AGAGTCCATC
    ATCCATAAGA AGATCATGAC TATCATCAAC TGCTTTATTA ATTCTAGTAT TCCACCAGCT
    TTACAAATTG ACATTCCAGT AGAGCAAGCC CAAAAAATTA TTGAACACAG GAAGGAGTTA
    GGACCATATG TGTTTAGAGA GGCACAGATG ACAATTTTTG GGGTTCTGTT TAAATTTTGG
    CCTCAGTTTT GTGAGTTTAG GAAGAACTTA ACTGATGAAA AAATTATGAG TGTTTTAGAG
    AGAAGACAAG AATATAATAA GCAGAAAAAA AAATTGGCAG TCATAGAAGA CGAAAATTTT
    GCAAAGGATG GAATCAAACA ATATCCAAGT TCTTCAGGAT CTGCAATCAA AACAGCTGCT
    TTAGCCACTG ACCCAGTTTT AGGCCTACAA GCATATGGCC GGCAGCCAAC CTGGTGCTAC
    TCCAAGTACA TAGAAGCCTT AGAGCAAGAA AGGATTCTTC TTAAAATTCA AGAGGAGCTG
    GAAAAAAAGT TATTTACAGC CACACCATCT TTTACAAACT TCAAGTCTAC TGCTTCCACT
    ATGTCTTTGA AAAAGACCGT ATCTTCCCAC AACATCCAGA CTTCCTGCAT TGAAGAAAGG
    AAGATGGGCC CTTTGGAGAC TTTTGCCAGA TTTCTTTCAC CAGCAATTTC AAGAGCAAAC
    ACTTGGAATA TCCTTGGTTA CCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39636
    Clone ID Related Accession (Same CDS sequence) XM_014118547.1
    Accession Version XM_014118547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GGATGGAATC
    AAACAATATC CAAGTTCTTC AGGATCTGCA ATCAAAACAG CTGCTTTAGC CACTGACCCA
    GTTTTAGGCC TACAAGCATA TGGCCGGCAG CCAACCTGGT GCTACTCCAA GTACATAGAA
    GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA ATTCAAGAGG AGCTGGAAAA AAAGTTATTT
    ACAGCCACAC CATCTTTTAC AAACTTCAAG TCTACTGCTT CCACTATGTC TTTGAAAAAG
    ACCGTATCTT CCCACAACAT CCAGACTTCC TGCATTGAAG AAAGGAAGAT GGGCCCTTTG
    GAGACTTTTG CCAGATTTCT TTCACCAGCA ATTTCAAGAG CAAACACTTG GAATATCCTT
    GGTTACCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974022.1
    CDS193..3504
    Translation

    Target ORF information:

    RefSeq Version XM_014118547.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACA CTACTTGTGA AATTGGAACC
    GGTATTTCAC GGCTTCCTGA GGTCTCTAAA AAAACAGAAT ACTGGAGTAA TGTTCCTGAA
    GAATATAGGC ACTTTAATTT TAATGACCTG CTTAACAACA AACTGGAGTT TGAACATTTC
    CGTCAGTTTC TTGAGGCTCA TTCTTCAAGT TTGGACCTTA TGTGCTGGAC AGACATTGAA
    CAGTTCCGAA GAATCATTTA TGGAGACCGA AAGCAGAGGG AAGCAAAATC TATATATATT
    AAAAACAAAT ACCTTAATAA AAAATATTTC TTTGGCCCCC AAAGTCCAGC TTCTCAATAT
    CAGCAGGACC AGATAATGAA TTTAAGTGGT GGCTGGGGAC AGATGCTCCA TGAGCAGCTT
    GATGCACCTG TTCTAATTGA AGTTCAGAAG CATGTTCTCA ATAAGCTAGA AAATGTGTGG
    TTGCCATTGT TTCTTGCAAG CGAACAGTTT GTAGCACGTC AAAAGATAAA GGATGGAATC
    AAACAATATC CAAGTTCTTC AGGATCTGCA ATCAAAACAG CTGCTTTAGC CACTGACCCA
    GTTTTAGGCC TACAAGCATA TGGCCGGCAG CCAACCTGGT GCTACTCCAA GTACATAGAA
    GCCTTAGAGC AAGAAAGGAT TCTTCTTAAA ATTCAAGAGG AGCTGGAAAA AAAGTTATTT
    ACAGCCACAC CATCTTTTAC AAACTTCAAG TCTACTGCTT CCACTATGTC TTTGAAAAAG
    ACCGTATCTT CCCACAACAT CCAGACTTCC TGCATTGAAG AAAGGAAGAT GGGCCCTTTG
    GAGACTTTTG CCAGATTTCT TTCACCAGCA ATTTCAAGAG CAAACACTTG GAATATCCTT
    GGTTACCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39637
    Clone ID Related Accession (Same CDS sequence) XM_014118548.1
    Accession Version XM_014118548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 22 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACT TGTGCCATTC TCATTGTGAA
    GAGTCCATCA TCCATAAGAA GATCATGACT ATCATCAACT GCTTTATTAA TTCTAGTATT
    CCACCAGCTT TACAAATTGA CATTCCAGTA GAGCAAGCCC AAAAAATTAT TGAACACAGG
    AAGGAGTTAG GACCATATGT GTTTAGAGAG GCACAGATGA CAATTTTTGG GGTTCTGTTT
    AAATTTTGGC CTCAGTTTTG TGAGTTTAGG AAGAACTTAA CTGATGAAAA AATTATGAGT
    GTTTTAGAGA GAAGACAAGA ATATAATAAG CAGAAAAAAA AATTGGCAGT CATAGAAGAC
    GAAAATTTTG CAAAGGATGG AATCAAACAA TATCCAAGTT CTTCAGGATC TGCAATCAAA
    ACAGCTGCTT TAGCCACTGA CCCAGTTTTA GGCCTACAAG CATATGGCCG GCAGCCAACC
    TGGTGCTACT CCAAGTACAT AGAAGCCTTA GAGCAAGAAA GGATTCTTCT TAAAATTCAA
    GAGGAGCTGG AAAAAAAGTT ATTTACAGCC ACACCATCTT TTACAAACTT CAAGTCTACT
    GCTTCCACTA TGTCTTTGAA AAAGACCGTA TCTTCCCACA ACATCCAGAC TTCCTGCATT
    GAAGAAAGGA AGATGGGCCC TTTGGAGACT TTTGCCAGAT TTCTTTCACC AGCAATTTCA
    AGAGCAAACA CTTGGAATAT CCTTGGTTAC CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974023.1
    CDS193..3348
    Translation

    Target ORF information:

    RefSeq Version XM_014118548.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 22 (RGS22), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCGGGA AGCTGCTGGC GGCGGAGCCA CCAGATATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCAA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGAT TATGGAGTTT TTGAAATAGT TAACAATGCT
    CCACAACTTC TAGAAAAGCA ACTGAAAAAA ATTCTTCGAA ACCAGCAACC TCGAAATCCC
    ATCTATGATG TTGTAAGGAA AGGAAAGAAT GATATTAAAC CTGTTCAGAT GAATGCCCCC
    TATGAAGATG AGGCCATTAA CGTCAACTAC AATATTATGT GTCTCAGTCG TGAAGAAGGT
    ATTGAGTGGA TCAAAAAAGA AAGACTTCCA GCATTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGATTAG CCAAATTGGT CTCACAAGTA AGATGGAGTG GATCCGGCAT GAACATCACA
    GTCAATACAG AATTTTCTCC CTGGGTCCTG AGAAAACCAC CCAGTCCACC ACCACCTGTT
    GTTGAAGAAG ACAATGTTAT AATTATGAAA AAGTTCTACA TTTCTCTTGG CGAGGGCTCC
    TATACTCAAA CAAAAGATTG GTTTGCATTA GCAAAACAGA GTTTGCAAAC AGTACCAACA
    TTTTCATTAC CATCTTGTGT ACTCCACAGC AAACCTGAAC CACCGGTTGT TTCATCCATT
    TCTGAAAGCT TTATCTTTGA TGATAGAATT CACCCAAGGA CAAAAAGGGA CTCATCTGAA
    ACCATTAGAT TAACTTCTCA TTTTGAAGAA GAAGAAGATG GGTCTGTATC TGTACAAGAC
    ACTCCTTCTC AAGCCCTTCT AAGAATATAC CTTGAGAGGC AACAGGATGC TGATGAAAGC
    CTTCCATTGC ATTTCACAAC ATGTGAAGAA TTTTTAAGAT CTTATATAGA TTTTATTTTG
    AGAGTAGCAA TTCATCAAAT TTTTGAAGAG CCACTTACAG AAAGTCCAGA TTATATAAAT
    TTCAACAAAG TAGCAGAAGT GGTGCTCGAG GAGGGTTTTG AGCCTCTCCA TGGCAGGAAT
    GTTTTAAGTG AAACATTTCA AACCACAAAT GAAAAGTCAA AAGAGATGTT AGAACGAGCA
    AATTCAAAGA GTGAGAGCAT TGGACCAGAA AGCAGGGCTT ATTGGTGTAT GTCTCACAAA
    GCTTATGATA TTGGCAATAG AAAGGAGTTT GAAAGATTTA AGAAATTTAT AAAAGGTACT
    TTAGGAGAGA GATATTGGTC ATTATGGATG GATATTGAGA GGTTAAAAGT TCTTAAGGAC
    CGCGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT TCTATCTAGT GAGCAATGGA
    GATTGCTATC TTTCTACAGA AATATTATCA AAATTTAAAC TGCTAGATGC ATCTCAGTGG
    AATGAAGAAC ACTTAAGAAA TATTCAGACA GAGGTTGTAA AACCATTACT TCTATATTGG
    GCACCAAGAT TCTGTGTTAC TCATTCATCC TCAGCTAAAC ATGCTAGCGC TGAACTGAAA
    TTCTGGCATC TCCGTCAAGA GAAACCAAGG AAGGACGTTG ACCCTTTTCC ACAAATGGCA
    ACCCTCTTGC CATTAAGACC TAAATCTTGC ATTCCACAGA TATCTGATAT GCAGAAAGAA
    GAATCCAACT TATTACAATG TCCTAAATCT CCCAAGAAAC CACCTAGAGT AAAAACAGCA
    GGTCAAAAAC CTTGGAAGAG CAAGTCTTTG TATCTAATTT CTTCGAAGGA TGATGCAATT
    GAGAAAGGGT TGAGGCACAG GTCAGAAAGC AACAAAGTTA TTCGCTTAAC ATCTTTTACT
    GACATTTCTG AATGTCTGAA GCCCCAGCTG GATAGAAGAT ACACTTACAC AGAAGAACCT
    TCGGTTAAAA CCATGACAGA TGGTGCCTTG GGAGGATTTG ACATGGAAAA TTTGTTGCAA
    TCTTTGTATG TAGAAAATAG AGCTGGATTC TTCTTTACTA AATTCTGTGA GCATTCAGGA
    AACAAGTTGT GGAAGAACAG TGTGTACTTT TGGTTTGACC TACAGGCTTA TCATCAGCTT
    TTCTACCAAG AAACACTTCA CCCTTTTAAA GTATGCAAAC AAGCCCAATA TCTTTTTGCC
    ACATACATTG CTCCTTCGGC TACTCTTGAC ATCGGACTCC AACAGGAAAA GAAAAAAGAA
    ATTTATATGA AAATACAGCC ACCATTTGAA GACCTTTTTG ACACAGCAGA AGAATATATC
    CTTCTCCTCC TCCTTGAGCC GTGGACAAAG ATGGTAAAAT TAGACCAAGT TGCTTTTGGA
    AAGGTGGAGC TGGTGGAAGA CACTCGACAG CTAGACTCCA CATACTTCAG AAAGCTACAG
    GCTTTGCATA AAGAGACCTT TTCCAAGAAA GCTGAGGACT TGTGCCATTC TCATTGTGAA
    GAGTCCATCA TCCATAAGAA GATCATGACT ATCATCAACT GCTTTATTAA TTCTAGTATT
    CCACCAGCTT TACAAATTGA CATTCCAGTA GAGCAAGCCC AAAAAATTAT TGAACACAGG
    AAGGAGTTAG GACCATATGT GTTTAGAGAG GCACAGATGA CAATTTTTGG GGTTCTGTTT
    AAATTTTGGC CTCAGTTTTG TGAGTTTAGG AAGAACTTAA CTGATGAAAA AATTATGAGT
    GTTTTAGAGA GAAGACAAGA ATATAATAAG CAGAAAAAAA AATTGGCAGT CATAGAAGAC
    GAAAATTTTG CAAAGGATGG AATCAAACAA TATCCAAGTT CTTCAGGATC TGCAATCAAA
    ACAGCTGCTT TAGCCACTGA CCCAGTTTTA GGCCTACAAG CATATGGCCG GCAGCCAACC
    TGGTGCTACT CCAAGTACAT AGAAGCCTTA GAGCAAGAAA GGATTCTTCT TAAAATTCAA
    GAGGAGCTGG AAAAAAAGTT ATTTACAGCC ACACCATCTT TTACAAACTT CAAGTCTACT
    GCTTCCACTA TGTCTTTGAA AAAGACCGTA TCTTCCCACA ACATCCAGAC TTCCTGCATT
    GAAGAAAGGA AGATGGGCCC TTTGGAGACT TTTGCCAGAT TTCTTTCACC AGCAATTTCA
    AGAGCAAACA CTTGGAATAT CCTTGGTTAC CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.