UBR2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005627359.2	XP_005627416.1	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_005627358.3	XP_005627415.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_005627359.3	XP_005627416.1	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_532138.6	XP_532138.2	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_022426054.1	XP_022281762.1	E3 ubiquitin-protein ligase UBR2 isoform X5
XM_022426053.1	XP_022281761.1	E3 ubiquitin-protein ligase UBR2 isoform X4
XM_005627360.3	XP_005627417.1	E3 ubiquitin-protein ligase UBR2 isoform X7
XM_532138.5	XP_532138.2	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_005627360.2	XP_005627417.1	E3 ubiquitin-protein ligase UBR2 isoform X4
XM_005627358.2	XP_005627415.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_022426055.1	XP_022281763.1	E3 ubiquitin-protein ligase UBR2 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe292462	XM_022426054.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe292463	XM_022426055.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe15271	XM_005627360.3 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe292461	XM_022426053.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe15268	XM_532138.6 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe15269	XM_005627358.3 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe15270	XM_005627359.3 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OCe15270	XM_005627359.2	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe15268	XM_532138.5	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe15271	XM_005627360.2	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe15269	XM_005627358.2	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

UBR2 ( XM_022426054.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281762.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe292462
Clone ID Related Accession (Same CDS sequence)	XM_022426054.1
Accession Version	XM_022426054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTATC TTGGCTGGGA 
AATGTTATTG GATATTCAGA TGGGCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GCGAAAACTC TTCTCTAGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTTATGA GCAGTCTACT TATGGATTTG 
AAATATAAGA AGTTGTTTGC TATTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAATCGCAG ACCTCTCGGT TCAGATATTC 
ACGGTCCCTT CACTTGCTCG GATGCTTATC ACAGAAGAAA ACCTGATGAC CATCATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA GTTTGAACGA 
TATACTGCTT TACAAGCCTT CAAGTTTAGG AGAGTTCAGA GCCTTATTTT TGATCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTAGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAAGTAGGAC AGCATATTGA AATGGAACCA GAGTGGGAGG CAGCCTTCAC ACTACAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GACTGGTGTG CTTTAGATGA AAAAGTGTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCTGTA TTGATGCAGT GTCATGGTGG TTTCACTGAT 
GGTGAACAAC CAATCACATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCCCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTACAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTAAGTGAA 
CTTAGCCCAC CCATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTA 
CATGCAGGAA TGTGGAGGAG AAATGGCTTC TCTCTAGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAG TAATGCTTCA GACAGGTGTC 
TCCATGATGG ATCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA TGGAAAAAGA TTTAGCTCTG AGATTACCCA TAAGGATGTA 
GTTCAGCAAA ACAATACTCT AATAGAAGAA ATGCTATACC TCATTATAAT GATTGTTGGA 
GAGAGATTTA GTCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAAT TAAGCATCAA ACCTATGGCT CATAGTGAAT TGGTGAAGTC TTTACCTGAA 
GACGAGAACA AGGAGACTGG CATGGAGAGC GTAATTGAAG CAGTTGCCCA TTTCAAAAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAGGAAGC TCAACGGAAA 
TTGAAAAGAC AAAACAGGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCTTTCTGC 
CCTCTATTTG CAAGTCTGGT AAACATTTTG CAGTCAGATG TGATGTTGTG CATCATGAGG 
ACAGTACTGC AATGGGCTGT AGAACATAAC GGATATGCGT GGTCTGAGTC CATGCTCCAA 
AGGGTGTTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCATTT AGACAATGTC 
ACAGAAGAGC ATGTAGTAAC ATTTACCTTC ACTCAGAAGA TATCAAAACC TGGTGAAGCA 
CCAAACAACT CCCCTAGCAT ATTAGCTATG CTGGAAACAC TACAGAATGC TCCCTACCTA 
GATGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATGCTAT TAAGAAGATA 
AGAGAGAGTT CATCCACCAG TCCTGTGGCA GAGACTGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TTATTGATGA GAACAAAGAA 
CTCTTTCAGC AGACATTAGA ACTAGATGCC TCTACTTCTG CTGTTCTTGA TAATAGCCCT 
ATGGTTTCAA ATATGGCACT TACAGCTTTG GGGCCAGCAC AAACTCAGGT CCCTGAACAG 
AGACAGTTTG TTACCTGTAT ATTGTGTCAA GAGGAGCAAG AGGTGAAGGT GGAGAGCAAG 
GCAATGGTTC TTGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA TAGGAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTGATCT GTCTTGCGGA 
ACACACACTG GTAGCTGTGG GCACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTGC GCTTACATAC CAGCTATGAT 
GTGGAAAACG GAGAATTCCT TTGTCCCCTC TGTGAGTGCT TGAGTAATAC TGTAATTCCT 
CTGCTGCTTC CTCCACGAAA TATTTTTAAC AGCAGGTTAA ATATTTCAGA CCAACCAAAA 
CTGACTCAGT GGATTAGAAC AATCTCTCAG CAAATAAAAG CATTACAGGT TCTTAGGAAA 
GAAGAAAGTA CTCCTAATAC TGCTTCTTCA AAGAATTTAG AGAATATGGA TGAATTGCAG 
TTCCCTGAAG GGTTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA GAGCATAAAA 
GAAATGCTAA CAACATTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAGGAGGATC CCCGTGTACC CATAATGTGT TGGGGTAGTT GTGCATACAC CATCCAAAGC 
ATAGAAAGAA TTTTAAGTGA TGAAGATAAA CCATTATTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCATT AACAAGATTT GCTGCAGCAC ATTGGACAGT GGCATCACTT 
TCAGTGGTGC AAGGACACTT TTGTAAACTT TTTGCATCAT TGGTGCCAGA TGACAACCAT 
GGAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCTG CATTGCAGTG TCAGGATTTT TCTGGGATCA GCCTTGGCAC TGGAGATCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATCGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAAATCCT GCTGGTGAAG AAGAATTAGC AGTTCTTGCT 
TTGTATAAAA CACTTCACCA GTGTACAGGA AGTGCCTTGA AAGAAATACC ATCTGGCTGG 
CATCTATGGA GGAATGTCAG AAATGGAATC ATGCCTTTCC TTAAGTGTTG CGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAACTTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TGTGTAACTA CCTTTCCCTG CCAAACAACC TCATTTGCCT TTTTCAAGAA 
AATAATGAGA TAACAAAATT ACTGATTGAA AGTTGGTGTC ATAACATTGA AGTTAAAAGA 
TATCTTGAAG GTGAAAGAGA TGCTATAAGC TATCCAAGAG AATCCAACAA ATTAATAGAC 
CTTCCAGAGG ATTATAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCATG CCCCAAATCA 
GGTGGTGATA AGAGCAGAGC CCCCACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC TGAACTGGAA GGGGAAGATG TGGGAGCCTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTCAGAG TGCGGGAATG TCAGGTGCTA 
TTTTTAGCTG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAAACTGACC AGGGACTCAG ACGGGGAAAT CCTTTACATT TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTTTGGCA CCAGCACAGT ATCACGGAGG AAATTGGACA TGCACAGGAA 
GCAAATCAGA CACTGGTTGG CATCGACTGG CAACATTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281762.1
CDS	159..4520
Translation	MHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNSRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_022426054.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426054.1

ORF Insert Sequence:

ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTATC TTGGCTGGGA 
AATGTTATTG GATATTCAGA TGGGCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GCGAAAACTC TTCTCTAGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTTATGA GCAGTCTACT TATGGATTTG 
AAATATAAGA AGTTGTTTGC TATTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAATCGCAG ACCTCTCGGT TCAGATATTC 
ACGGTCCCTT CACTTGCTCG GATGCTTATC ACAGAAGAAA ACCTGATGAC CATCATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA GTTTGAACGA 
TATACTGCTT TACAAGCCTT CAAGTTTAGG AGAGTTCAGA GCCTTATTTT TGATCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTAGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAAGTAGGAC AGCATATTGA AATGGAACCA GAGTGGGAGG CAGCCTTCAC ACTACAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GACTGGTGTG CTTTAGATGA AAAAGTGTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCTGTA TTGATGCAGT GTCATGGTGG TTTCACTGAT 
GGTGAACAAC CAATCACATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCCCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTACAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTAAGTGAA 
CTTAGCCCAC CCATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTA 
CATGCAGGAA TGTGGAGGAG AAATGGCTTC TCTCTAGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAG TAATGCTTCA GACAGGTGTC 
TCCATGATGG ATCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA TGGAAAAAGA TTTAGCTCTG AGATTACCCA TAAGGATGTA 
GTTCAGCAAA ACAATACTCT AATAGAAGAA ATGCTATACC TCATTATAAT GATTGTTGGA 
GAGAGATTTA GTCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAAT TAAGCATCAA ACCTATGGCT CATAGTGAAT TGGTGAAGTC TTTACCTGAA 
GACGAGAACA AGGAGACTGG CATGGAGAGC GTAATTGAAG CAGTTGCCCA TTTCAAAAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAGGAAGC TCAACGGAAA 
TTGAAAAGAC AAAACAGGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCTTTCTGC 
CCTCTATTTG CAAGTCTGGT AAACATTTTG CAGTCAGATG TGATGTTGTG CATCATGAGG 
ACAGTACTGC AATGGGCTGT AGAACATAAC GGATATGCGT GGTCTGAGTC CATGCTCCAA 
AGGGTGTTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCATTT AGACAATGTC 
ACAGAAGAGC ATGTAGTAAC ATTTACCTTC ACTCAGAAGA TATCAAAACC TGGTGAAGCA 
CCAAACAACT CCCCTAGCAT ATTAGCTATG CTGGAAACAC TACAGAATGC TCCCTACCTA 
GATGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATGCTAT TAAGAAGATA 
AGAGAGAGTT CATCCACCAG TCCTGTGGCA GAGACTGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TTATTGATGA GAACAAAGAA 
CTCTTTCAGC AGACATTAGA ACTAGATGCC TCTACTTCTG CTGTTCTTGA TAATAGCCCT 
ATGGTTTCAA ATATGGCACT TACAGCTTTG GGGCCAGCAC AAACTCAGGT CCCTGAACAG 
AGACAGTTTG TTACCTGTAT ATTGTGTCAA GAGGAGCAAG AGGTGAAGGT GGAGAGCAAG 
GCAATGGTTC TTGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA TAGGAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTGATCT GTCTTGCGGA 
ACACACACTG GTAGCTGTGG GCACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTGC GCTTACATAC CAGCTATGAT 
GTGGAAAACG GAGAATTCCT TTGTCCCCTC TGTGAGTGCT TGAGTAATAC TGTAATTCCT 
CTGCTGCTTC CTCCACGAAA TATTTTTAAC AGCAGGTTAA ATATTTCAGA CCAACCAAAA 
CTGACTCAGT GGATTAGAAC AATCTCTCAG CAAATAAAAG CATTACAGGT TCTTAGGAAA 
GAAGAAAGTA CTCCTAATAC TGCTTCTTCA AAGAATTTAG AGAATATGGA TGAATTGCAG 
TTCCCTGAAG GGTTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA GAGCATAAAA 
GAAATGCTAA CAACATTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAGGAGGATC CCCGTGTACC CATAATGTGT TGGGGTAGTT GTGCATACAC CATCCAAAGC 
ATAGAAAGAA TTTTAAGTGA TGAAGATAAA CCATTATTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCATT AACAAGATTT GCTGCAGCAC ATTGGACAGT GGCATCACTT 
TCAGTGGTGC AAGGACACTT TTGTAAACTT TTTGCATCAT TGGTGCCAGA TGACAACCAT 
GGAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCTG CATTGCAGTG TCAGGATTTT TCTGGGATCA GCCTTGGCAC TGGAGATCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATCGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAAATCCT GCTGGTGAAG AAGAATTAGC AGTTCTTGCT 
TTGTATAAAA CACTTCACCA GTGTACAGGA AGTGCCTTGA AAGAAATACC ATCTGGCTGG 
CATCTATGGA GGAATGTCAG AAATGGAATC ATGCCTTTCC TTAAGTGTTG CGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAACTTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TGTGTAACTA CCTTTCCCTG CCAAACAACC TCATTTGCCT TTTTCAAGAA 
AATAATGAGA TAACAAAATT ACTGATTGAA AGTTGGTGTC ATAACATTGA AGTTAAAAGA 
TATCTTGAAG GTGAAAGAGA TGCTATAAGC TATCCAAGAG AATCCAACAA ATTAATAGAC 
CTTCCAGAGG ATTATAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCATG CCCCAAATCA 
GGTGGTGATA AGAGCAGAGC CCCCACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC TGAACTGGAA GGGGAAGATG TGGGAGCCTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTCAGAG TGCGGGAATG TCAGGTGCTA 
TTTTTAGCTG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAAACTGACC AGGGACTCAG ACGGGGAAAT CCTTTACATT TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTTTGGCA CCAGCACAGT ATCACGGAGG AAATTGGACA TGCACAGGAA 
GCAAATCAGA CACTGGTTGG CATCGACTGG CAACATTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_022426055.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281763.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe292463
Clone ID Related Accession (Same CDS sequence)	XM_022426055.1
Accession Version	XM_022426055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGAT ACTGCTTCTT CAAAGAATTT 
AGAGAATATG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281763.1
CDS	61..3795
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNRYCFFKEFREYG

Target ORF information:

RefSeq Version	XM_022426055.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426055.1

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGAT ACTGCTTCTT CAAAGAATTT 
AGAGAATATG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005627360.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627417.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X7, mRNA.

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CloneID	OCe15271
Clone ID Related Accession (Same CDS sequence)	XM_005627360.3 , XM_005627360.2
Accession Version	XM_005627360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627417.1
CDS	61..3600
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQS

Target ORF information:

RefSeq Version	XM_005627360.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627360.3

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005627360.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627417.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

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CloneID	OCe15271
Clone ID Related Accession (Same CDS sequence)	XM_005627360.3 , XM_005627360.2
Accession Version	XM_005627360.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627417.1
CDS	474..4013
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQS

Target ORF information:

RefSeq Version	XM_005627360.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627360.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_022426053.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281761.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe292461
Clone ID Related Accession (Same CDS sequence)	XM_022426053.1
Accession Version	XM_022426053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAT GGCTGCAAGC AACTGACCTC ACTAGAGAAG TGTACCAGCA TTTAGCCCAC 
TATGTACCCA AAATCTACTG CAGGGGTCCC AACCCTTTTC CACAGAAGGA GGACATGCTG 
GCACAGCATG TTTTGTTGGG ACCAATGGAA TGGTACCTTT GTGGTGAAGA TCCCGCATTT 
GGATTTCCAA AACTTGAACA AGCAAATAAA CCTTCTCATC TTTGTGGTCG TGTTTTTAAA 
GTAGGAGAGC CTACATACTC TTGCAGAGAC TGTGCAGTTG ATCCAACTTG TGTTTTATGC 
ATGGAGTGCT TTTTGGGAAG TATTCACAGA GACCATCGAT ATAGGATGAC AACATCAGGA 
GGTGGAGGTT TCTGTGACTG TGGTGATACT GAAGCTTGGA AAGAGGGTCC TTACTGTCAA 
AAACATGAAC TTAACACTTC TGAAATTGAG GAAGAAGAGG ATCCTCTTGT CCATTTATCA 
GAGGATGTGA TAGCGAGAAC CTACAACATT TTTGCTATTA TGTTTCGATA TGCAGTGGAG 
ATACTAACCT GGGAAAAAGA AAGTGAATTG CCAGCAGATT TAGAGATAGT AGAGAAGAGT 
GATACCTACT ATTGCATGCT GTTTAATGAT GAGGTGCATA CTTATGAACA AGTTATTTAT 
ACCCTTCAGA AAGCTGTTAA CTGTACACAA AAAGAAGCCA TTGGCTTTGC AACTACAGTA 
GATCGAGATG GACGTAGGTC TGTTCGATAT GGAGATTTCC AGTATTGTGA ACAAGCAAAA 
TCAGTAATTG TGAGAAATAC CAGTAGACAG ACAAAGCCAC TCAAAGTTCA AGTTATGCAT 
TCGTCTATTG TCGCACATCA GAATTTTGGT TTGAAACTTT TATCTTGGCT GGGAAATGTT 
ATTGGATATT CAGATGGGCT TCGCCGAATT TTATGTCAAG TTGGTTTACA AGAAGGGCCA 
GATGGCGAAA ACTCTTCTCT AGTGGATAGA TTGATGCTTA ATGATTCCAA ATTGTGGAAA 
GGTGCTAGGA GTGTGTATCA TCAGTTGTTT ATGAGCAGTC TACTTATGGA TTTGAAATAT 
AAGAAGTTGT TTGCTATTCG ATTTGCAAAG AACTATGAGC GTTTGCAGAG TGATTATGTG 
ACAGATGACC ACGACAGAGA GTTTTCAATC GCAGACCTCT CGGTTCAGAT ATTCACGGTC 
CCTTCACTTG CTCGGATGCT TATCACAGAA GAAAACCTGA TGACCATCAT CATTAAGACT 
TTTATGGATC ATTTGAGACA TCGAGATGCC CAGGGCAGAT TTCAGTTTGA ACGATATACT 
GCTTTACAAG CCTTCAAGTT TAGGAGAGTT CAGAGCCTTA TTTTTGATCT CAAGTATGTG 
TTAATTAGCA AACCAACTGA ATGGTCAGAT GATCTGAGGC AGAAGTTCCT AGAAGGATTT 
GATGCCTTTT TGGAATTACT CAAATGTATG CAGGGAATGG ATCCAATTAC ACGTCAAGTA 
GGACAGCATA TTGAAATGGA ACCAGAGTGG GAGGCAGCCT TCACACTACA AATGAAATTA 
ACACATGTCA TTTCAATGAT GCAGGACTGG TGTGCTTTAG ATGAAAAAGT GTTAATTGAA 
GCTTACAAGA AATGCCTTGC TGTATTGATG CAGTGTCATG GTGGTTTCAC TGATGGTGAA 
CAACCAATCA CATTAAGCAT TTGTGGACAT TCAGTGGAAA CTATCAGATA CTGTGTTTCC 
CAAGAAAAAG TTAGCATTCA CCTCCCAGTT TCTCGCTTAC TTGCAGGTTT ACATGTATTA 
TTAAGCAAAA GTGAAGTGGC ATATAAATTT CCAGAGCTCC TACCTCTAAG TGAACTTAGC 
CCACCCATGT TGATAGAACA CCCTCTTAGA TGTCTTGTTT TATGTGCCCA AGTACATGCA 
GGAATGTGGA GGAGAAATGG CTTCTCTCTA GTAAACCAGA TTTATTACTA CCATAATGTG 
AAATGCAGAC GTGAAATGTT TGACAAGGAT ATAGTAATGC TTCAGACAGG TGTCTCCATG 
ATGGATCCAA ATCATTTCCT GATGATAATG CTCAGCCGCT TTGAACTTTA TCAGATTTTC 
AGTACTCCAG ACTATGGAAA AAGATTTAGC TCTGAGATTA CCCATAAGGA TGTAGTTCAG 
CAAAACAATA CTCTAATAGA AGAAATGCTA TACCTCATTA TAATGATTGT TGGAGAGAGA 
TTTAGTCCTG GAGTTGGACA GGTAAATGCT ACAGATGAAA TTAAGCGAGA GATTATCCAT 
CAATTAAGCA TCAAACCTAT GGCTCATAGT GAATTGGTGA AGTCTTTACC TGAAGACGAG 
AACAAGGAGA CTGGCATGGA GAGCGTAATT GAAGCAGTTG CCCATTTCAA AAAACCTGGA 
TTAACAGGAC GAGGCATGTA TGAACTGAAA CCAGAGTGTG CCAAAGAGTT CAATTTGTAT 
TTCTATCACT TTTCAAGGGC AGAGCAGTCC AAGGCAGAGG AAGCTCAACG GAAATTGAAA 
AGACAAAACA GGGAAGATAC AGCACTTCCA CCTCCAGTTT TGCCTCCTTT CTGCCCTCTA 
TTTGCAAGTC TGGTAAACAT TTTGCAGTCA GATGTGATGT TGTGCATCAT GAGGACAGTA 
CTGCAATGGG CTGTAGAACA TAACGGATAT GCGTGGTCTG AGTCCATGCT CCAAAGGGTG 
TTACATTTAA TTGGAATGGC ACTACAAGAA GAAAAACAGC ATTTAGACAA TGTCACAGAA 
GAGCATGTAG TAACATTTAC CTTCACTCAG AAGATATCAA AACCTGGTGA AGCACCAAAC 
AACTCCCCTA GCATATTAGC TATGCTGGAA ACACTACAGA ATGCTCCCTA CCTAGATGTC 
CACAAAGATA TGATTAGATG GATATTAAAG ACTTTTAATG CTATTAAGAA GATAAGAGAG 
AGTTCATCCA CCAGTCCTGT GGCAGAGACT GAAGGAACCA TAATGGAAGA GAGTTCAAGA 
GACAAAGACA AAGCTGAGAG GAAGAGAAAA GCTGAGATTG CCAGACTGCG CAGAGAAAAG 
ATCATGGCCC AGATGTCTGA GATGCAGCGG CACTTTATTG ATGAGAACAA AGAACTCTTT 
CAGCAGACAT TAGAACTAGA TGCCTCTACT TCTGCTGTTC TTGATAATAG CCCTATGGTT 
TCAAATATGG CACTTACAGC TTTGGGGCCA GCACAAACTC AGGTCCCTGA ACAGAGACAG 
TTTGTTACCT GTATATTGTG TCAAGAGGAG CAAGAGGTGA AGGTGGAGAG CAAGGCAATG 
GTTCTTGCAG CATTTGTTCA AAGATCAACT GTATTATCAA AAAATAGGAG TAAATTCATT 
CAGGATCCAG AAAAATATGA TCCATTATTC ATGCACCCTG ATCTGTCTTG CGGAACACAC 
ACTGGTAGCT GTGGGCACAT TATGCATGCC CATTGTTGGC AAAGGTATTT TGATTCCGTT 
CAAGCTAAAG AACAGCGAAG GCAACAGAGA TTGCGCTTAC ATACCAGCTA TGATGTGGAA 
AACGGAGAAT TCCTTTGTCC CCTCTGTGAG TGCTTGAGTA ATACTGTAAT TCCTCTGCTG 
CTTCCTCCAC GAAATATTTT TAACAGCAGG TTAAATATTT CAGACCAACC AAAACTGACT 
CAGTGGATTA GAACAATCTC TCAGCAAATA AAAGCATTAC AGGTTCTTAG GAAAGAAGAA 
AGTACTCCTA ATACTGCTTC TTCAAAGAAT TTAGAGAATA TGGATGAATT GCAGTTCCCT 
GAAGGGTTTA GGCCTGATTT TCATCCTAAG AACCCTTATT CTGAGAGCAT AAAAGAAATG 
CTAACAACAT TTGGAACTGC TACTTACAAG GTGGGACTAA AGGTTCATCC CAATGAGGAG 
GATCCCCGTG TACCCATAAT GTGTTGGGGT AGTTGTGCAT ACACCATCCA AAGCATAGAA 
AGAATTTTAA GTGATGAAGA TAAACCATTA TTTGGTCCTT TGCCTTGCAG ACTGGATGAC 
TGTCTTAGGT CATTAACAAG ATTTGCTGCA GCACATTGGA CAGTGGCATC ACTTTCAGTG 
GTGCAAGGAC ACTTTTGTAA ACTTTTTGCA TCATTGGTGC CAGATGACAA CCATGGAGAC 
CTTCCATGCA TATTAGATAT CGACATGTTT CATTTATTGG TGGGCTTGGT GCTCGCTTTC 
CCTGCATTGC AGTGTCAGGA TTTTTCTGGG ATCAGCCTTG GCACTGGAGA TCTTCACATT 
TTCCATCTGG TTACTATGGC ACACATCGTA CAGATCTTAC TTACCTCGTG TACAGAAGAG 
AATGGCATGG ATCAAGAAAA TCCTGCTGGT GAAGAAGAAT TAGCAGTTCT TGCTTTGTAT 
AAAACACTTC ACCAGTGTAC AGGAAGTGCC TTGAAAGAAA TACCATCTGG CTGGCATCTA 
TGGAGGAATG TCAGAAATGG AATCATGCCT TTCCTTAAGT GTTGCGCTTT GTTTTTTCAT 
TACTTAAATG GAGTTCCTTC TCCACCTGAA CTTCAAGTTT CTGGAACAAG CCATTTTGAA 
CATTTGTGTA ACTACCTTTC CCTGCCAAAC AACCTCATTT GCCTTTTTCA AGAAAATAAT 
GAGATAACAA AATTACTGAT TGAAAGTTGG TGTCATAACA TTGAAGTTAA AAGATATCTT 
GAAGGTGAAA GAGATGCTAT AAGCTATCCA AGAGAATCCA ACAAATTAAT AGACCTTCCA 
GAGGATTATA GCAGCCTCAT TAATCAAGCA TCTAATTTCT CATGCCCCAA ATCAGGTGGT 
GATAAGAGCA GAGCCCCCAC TCTGTGCCTT GTGTGTGGAA CTCTGCTGTG CTCCCAGAGC 
TACTGCTGTC AGACTGAACT GGAAGGGGAA GATGTGGGAG CCTGCACAGC TCACACCTAC 
TCCTGTGGTT CTGGGGTGGG CATCTTCCTC AGAGTGCGGG AATGTCAGGT GCTATTTTTA 
GCTGGCAAAA CCAAAGGCTG TTTTTATTCT CCTCCTTACC TTGATGACTA TGGGGAAACT 
GACCAGGGAC TCAGACGGGG AAATCCTTTA CATTTGTGCA AAGAGCGATT TAAGAAGATT 
CAGAAGCTTT GGCACCAGCA CAGTATCACG GAGGAAATTG GACATGCACA GGAAGCAAAT 
CAGACACTGG TTGGCATCGA CTGGCAACAT TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281761.1
CDS	204..5399
Translation	MTKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNSRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_022426053.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426053.1

ORF Insert Sequence:

ATGACAAAAT GGCTGCAAGC AACTGACCTC ACTAGAGAAG TGTACCAGCA TTTAGCCCAC 
TATGTACCCA AAATCTACTG CAGGGGTCCC AACCCTTTTC CACAGAAGGA GGACATGCTG 
GCACAGCATG TTTTGTTGGG ACCAATGGAA TGGTACCTTT GTGGTGAAGA TCCCGCATTT 
GGATTTCCAA AACTTGAACA AGCAAATAAA CCTTCTCATC TTTGTGGTCG TGTTTTTAAA 
GTAGGAGAGC CTACATACTC TTGCAGAGAC TGTGCAGTTG ATCCAACTTG TGTTTTATGC 
ATGGAGTGCT TTTTGGGAAG TATTCACAGA GACCATCGAT ATAGGATGAC AACATCAGGA 
GGTGGAGGTT TCTGTGACTG TGGTGATACT GAAGCTTGGA AAGAGGGTCC TTACTGTCAA 
AAACATGAAC TTAACACTTC TGAAATTGAG GAAGAAGAGG ATCCTCTTGT CCATTTATCA 
GAGGATGTGA TAGCGAGAAC CTACAACATT TTTGCTATTA TGTTTCGATA TGCAGTGGAG 
ATACTAACCT GGGAAAAAGA AAGTGAATTG CCAGCAGATT TAGAGATAGT AGAGAAGAGT 
GATACCTACT ATTGCATGCT GTTTAATGAT GAGGTGCATA CTTATGAACA AGTTATTTAT 
ACCCTTCAGA AAGCTGTTAA CTGTACACAA AAAGAAGCCA TTGGCTTTGC AACTACAGTA 
GATCGAGATG GACGTAGGTC TGTTCGATAT GGAGATTTCC AGTATTGTGA ACAAGCAAAA 
TCAGTAATTG TGAGAAATAC CAGTAGACAG ACAAAGCCAC TCAAAGTTCA AGTTATGCAT 
TCGTCTATTG TCGCACATCA GAATTTTGGT TTGAAACTTT TATCTTGGCT GGGAAATGTT 
ATTGGATATT CAGATGGGCT TCGCCGAATT TTATGTCAAG TTGGTTTACA AGAAGGGCCA 
GATGGCGAAA ACTCTTCTCT AGTGGATAGA TTGATGCTTA ATGATTCCAA ATTGTGGAAA 
GGTGCTAGGA GTGTGTATCA TCAGTTGTTT ATGAGCAGTC TACTTATGGA TTTGAAATAT 
AAGAAGTTGT TTGCTATTCG ATTTGCAAAG AACTATGAGC GTTTGCAGAG TGATTATGTG 
ACAGATGACC ACGACAGAGA GTTTTCAATC GCAGACCTCT CGGTTCAGAT ATTCACGGTC 
CCTTCACTTG CTCGGATGCT TATCACAGAA GAAAACCTGA TGACCATCAT CATTAAGACT 
TTTATGGATC ATTTGAGACA TCGAGATGCC CAGGGCAGAT TTCAGTTTGA ACGATATACT 
GCTTTACAAG CCTTCAAGTT TAGGAGAGTT CAGAGCCTTA TTTTTGATCT CAAGTATGTG 
TTAATTAGCA AACCAACTGA ATGGTCAGAT GATCTGAGGC AGAAGTTCCT AGAAGGATTT 
GATGCCTTTT TGGAATTACT CAAATGTATG CAGGGAATGG ATCCAATTAC ACGTCAAGTA 
GGACAGCATA TTGAAATGGA ACCAGAGTGG GAGGCAGCCT TCACACTACA AATGAAATTA 
ACACATGTCA TTTCAATGAT GCAGGACTGG TGTGCTTTAG ATGAAAAAGT GTTAATTGAA 
GCTTACAAGA AATGCCTTGC TGTATTGATG CAGTGTCATG GTGGTTTCAC TGATGGTGAA 
CAACCAATCA CATTAAGCAT TTGTGGACAT TCAGTGGAAA CTATCAGATA CTGTGTTTCC 
CAAGAAAAAG TTAGCATTCA CCTCCCAGTT TCTCGCTTAC TTGCAGGTTT ACATGTATTA 
TTAAGCAAAA GTGAAGTGGC ATATAAATTT CCAGAGCTCC TACCTCTAAG TGAACTTAGC 
CCACCCATGT TGATAGAACA CCCTCTTAGA TGTCTTGTTT TATGTGCCCA AGTACATGCA 
GGAATGTGGA GGAGAAATGG CTTCTCTCTA GTAAACCAGA TTTATTACTA CCATAATGTG 
AAATGCAGAC GTGAAATGTT TGACAAGGAT ATAGTAATGC TTCAGACAGG TGTCTCCATG 
ATGGATCCAA ATCATTTCCT GATGATAATG CTCAGCCGCT TTGAACTTTA TCAGATTTTC 
AGTACTCCAG ACTATGGAAA AAGATTTAGC TCTGAGATTA CCCATAAGGA TGTAGTTCAG 
CAAAACAATA CTCTAATAGA AGAAATGCTA TACCTCATTA TAATGATTGT TGGAGAGAGA 
TTTAGTCCTG GAGTTGGACA GGTAAATGCT ACAGATGAAA TTAAGCGAGA GATTATCCAT 
CAATTAAGCA TCAAACCTAT GGCTCATAGT GAATTGGTGA AGTCTTTACC TGAAGACGAG 
AACAAGGAGA CTGGCATGGA GAGCGTAATT GAAGCAGTTG CCCATTTCAA AAAACCTGGA 
TTAACAGGAC GAGGCATGTA TGAACTGAAA CCAGAGTGTG CCAAAGAGTT CAATTTGTAT 
TTCTATCACT TTTCAAGGGC AGAGCAGTCC AAGGCAGAGG AAGCTCAACG GAAATTGAAA 
AGACAAAACA GGGAAGATAC AGCACTTCCA CCTCCAGTTT TGCCTCCTTT CTGCCCTCTA 
TTTGCAAGTC TGGTAAACAT TTTGCAGTCA GATGTGATGT TGTGCATCAT GAGGACAGTA 
CTGCAATGGG CTGTAGAACA TAACGGATAT GCGTGGTCTG AGTCCATGCT CCAAAGGGTG 
TTACATTTAA TTGGAATGGC ACTACAAGAA GAAAAACAGC ATTTAGACAA TGTCACAGAA 
GAGCATGTAG TAACATTTAC CTTCACTCAG AAGATATCAA AACCTGGTGA AGCACCAAAC 
AACTCCCCTA GCATATTAGC TATGCTGGAA ACACTACAGA ATGCTCCCTA CCTAGATGTC 
CACAAAGATA TGATTAGATG GATATTAAAG ACTTTTAATG CTATTAAGAA GATAAGAGAG 
AGTTCATCCA CCAGTCCTGT GGCAGAGACT GAAGGAACCA TAATGGAAGA GAGTTCAAGA 
GACAAAGACA AAGCTGAGAG GAAGAGAAAA GCTGAGATTG CCAGACTGCG CAGAGAAAAG 
ATCATGGCCC AGATGTCTGA GATGCAGCGG CACTTTATTG ATGAGAACAA AGAACTCTTT 
CAGCAGACAT TAGAACTAGA TGCCTCTACT TCTGCTGTTC TTGATAATAG CCCTATGGTT 
TCAAATATGG CACTTACAGC TTTGGGGCCA GCACAAACTC AGGTCCCTGA ACAGAGACAG 
TTTGTTACCT GTATATTGTG TCAAGAGGAG CAAGAGGTGA AGGTGGAGAG CAAGGCAATG 
GTTCTTGCAG CATTTGTTCA AAGATCAACT GTATTATCAA AAAATAGGAG TAAATTCATT 
CAGGATCCAG AAAAATATGA TCCATTATTC ATGCACCCTG ATCTGTCTTG CGGAACACAC 
ACTGGTAGCT GTGGGCACAT TATGCATGCC CATTGTTGGC AAAGGTATTT TGATTCCGTT 
CAAGCTAAAG AACAGCGAAG GCAACAGAGA TTGCGCTTAC ATACCAGCTA TGATGTGGAA 
AACGGAGAAT TCCTTTGTCC CCTCTGTGAG TGCTTGAGTA ATACTGTAAT TCCTCTGCTG 
CTTCCTCCAC GAAATATTTT TAACAGCAGG TTAAATATTT CAGACCAACC AAAACTGACT 
CAGTGGATTA GAACAATCTC TCAGCAAATA AAAGCATTAC AGGTTCTTAG GAAAGAAGAA 
AGTACTCCTA ATACTGCTTC TTCAAAGAAT TTAGAGAATA TGGATGAATT GCAGTTCCCT 
GAAGGGTTTA GGCCTGATTT TCATCCTAAG AACCCTTATT CTGAGAGCAT AAAAGAAATG 
CTAACAACAT TTGGAACTGC TACTTACAAG GTGGGACTAA AGGTTCATCC CAATGAGGAG 
GATCCCCGTG TACCCATAAT GTGTTGGGGT AGTTGTGCAT ACACCATCCA AAGCATAGAA 
AGAATTTTAA GTGATGAAGA TAAACCATTA TTTGGTCCTT TGCCTTGCAG ACTGGATGAC 
TGTCTTAGGT CATTAACAAG ATTTGCTGCA GCACATTGGA CAGTGGCATC ACTTTCAGTG 
GTGCAAGGAC ACTTTTGTAA ACTTTTTGCA TCATTGGTGC CAGATGACAA CCATGGAGAC 
CTTCCATGCA TATTAGATAT CGACATGTTT CATTTATTGG TGGGCTTGGT GCTCGCTTTC 
CCTGCATTGC AGTGTCAGGA TTTTTCTGGG ATCAGCCTTG GCACTGGAGA TCTTCACATT 
TTCCATCTGG TTACTATGGC ACACATCGTA CAGATCTTAC TTACCTCGTG TACAGAAGAG 
AATGGCATGG ATCAAGAAAA TCCTGCTGGT GAAGAAGAAT TAGCAGTTCT TGCTTTGTAT 
AAAACACTTC ACCAGTGTAC AGGAAGTGCC TTGAAAGAAA TACCATCTGG CTGGCATCTA 
TGGAGGAATG TCAGAAATGG AATCATGCCT TTCCTTAAGT GTTGCGCTTT GTTTTTTCAT 
TACTTAAATG GAGTTCCTTC TCCACCTGAA CTTCAAGTTT CTGGAACAAG CCATTTTGAA 
CATTTGTGTA ACTACCTTTC CCTGCCAAAC AACCTCATTT GCCTTTTTCA AGAAAATAAT 
GAGATAACAA AATTACTGAT TGAAAGTTGG TGTCATAACA TTGAAGTTAA AAGATATCTT 
GAAGGTGAAA GAGATGCTAT AAGCTATCCA AGAGAATCCA ACAAATTAAT AGACCTTCCA 
GAGGATTATA GCAGCCTCAT TAATCAAGCA TCTAATTTCT CATGCCCCAA ATCAGGTGGT 
GATAAGAGCA GAGCCCCCAC TCTGTGCCTT GTGTGTGGAA CTCTGCTGTG CTCCCAGAGC 
TACTGCTGTC AGACTGAACT GGAAGGGGAA GATGTGGGAG CCTGCACAGC TCACACCTAC 
TCCTGTGGTT CTGGGGTGGG CATCTTCCTC AGAGTGCGGG AATGTCAGGT GCTATTTTTA 
GCTGGCAAAA CCAAAGGCTG TTTTTATTCT CCTCCTTACC TTGATGACTA TGGGGAAACT 
GACCAGGGAC TCAGACGGGG AAATCCTTTA CATTTGTGCA AAGAGCGATT TAAGAAGATT 
CAGAAGCTTT GGCACCAGCA CAGTATCACG GAGGAAATTG GACATGCACA GGAAGCAAAT 
CAGACACTGG TTGGCATCGA CTGGCAACAT TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_532138.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532138.2 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

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CloneID	OCe15268
Clone ID Related Accession (Same CDS sequence)	XM_532138.6 , XM_532138.5
Accession Version	XM_532138.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532138.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGAGCGA GCAGTGTCCG TGACTGCTCT GTCTGTCCAG 
TTCTTCACCG CACCTACTCT GGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532138.2
CDS	61..5328
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYQQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNSRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_532138.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532138.6

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGAGCGA GCAGTGTCCG TGACTGCTCT GTCTGTCCAG 
TTCTTCACCG CACCTACTCT GGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_532138.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532138.2 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

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CloneID	OCe15268
Clone ID Related Accession (Same CDS sequence)	XM_532138.6 , XM_532138.5
Accession Version	XM_532138.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532138.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGAGCGA GCAGTGTCCG TGACTGCTCT GTCTGTCCAG 
TTCTTCACCG CACCTACTCT GGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532138.2
CDS	472..5739
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYQQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNSRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_532138.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532138.5

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAATTACCA GCAGTTGCAG 
AGAGATTTTA TGGAGGATGA TCACGAGCGA GCAGTGTCCG TGACTGCTCT GTCTGTCCAG 
TTCTTCACCG CACCTACTCT GGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005627358.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627415.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

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CloneID	OCe15269
Clone ID Related Accession (Same CDS sequence)	XM_005627358.3 , XM_005627358.2
Accession Version	XM_005627358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627415.1
CDS	61..5328
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNSRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005627358.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627358.3

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005627358.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627415.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

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CloneID	OCe15269
Clone ID Related Accession (Same CDS sequence)	XM_005627358.3 , XM_005627358.2
Accession Version	XM_005627358.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627415.1
CDS	472..5739
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNSRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005627358.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627358.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGCA GGTTAAATAT TTCAGACCAA 
CCAAAACTGA CTCAGTGGAT TAGAACAATC TCTCAGCAAA TAAAAGCATT ACAGGTTCTT 
AGGAAAGAAG AAAGTACTCC TAATACTGCT TCTTCAAAGA ATTTAGAGAA TATGGATGAA 
TTGCAGTTCC CTGAAGGGTT TAGGCCTGAT TTTCATCCTA AGAACCCTTA TTCTGAGAGC 
ATAAAAGAAA TGCTAACAAC ATTTGGAACT GCTACTTACA AGGTGGGACT AAAGGTTCAT 
CCCAATGAGG AGGATCCCCG TGTACCCATA ATGTGTTGGG GTAGTTGTGC ATACACCATC 
CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TATTTGGTCC TTTGCCTTGC 
AGACTGGATG ACTGTCTTAG GTCATTAACA AGATTTGCTG CAGCACATTG GACAGTGGCA 
TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCAGATGAC 
AACCATGGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG 
GTGCTCGCTT TCCCTGCATT GCAGTGTCAG GATTTTTCTG GGATCAGCCT TGGCACTGGA 
GATCTTCACA TTTTCCATCT GGTTACTATG GCACACATCG TACAGATCTT ACTTACCTCG 
TGTACAGAAG AGAATGGCAT GGATCAAGAA AATCCTGCTG GTGAAGAAGA ATTAGCAGTT 
CTTGCTTTGT ATAAAACACT TCACCAGTGT ACAGGAAGTG CCTTGAAAGA AATACCATCT 
GGCTGGCATC TATGGAGGAA TGTCAGAAAT GGAATCATGC CTTTCCTTAA GTGTTGCGCT 
TTGTTTTTTC ATTACTTAAA TGGAGTTCCT TCTCCACCTG AACTTCAAGT TTCTGGAACA 
AGCCATTTTG AACATTTGTG TAACTACCTT TCCCTGCCAA ACAACCTCAT TTGCCTTTTT 
CAAGAAAATA ATGAGATAAC AAAATTACTG ATTGAAAGTT GGTGTCATAA CATTGAAGTT 
AAAAGATATC TTGAAGGTGA AAGAGATGCT ATAAGCTATC CAAGAGAATC CAACAAATTA 
ATAGACCTTC CAGAGGATTA TAGCAGCCTC ATTAATCAAG CATCTAATTT CTCATGCCCC 
AAATCAGGTG GTGATAAGAG CAGAGCCCCC ACTCTGTGCC TTGTGTGTGG AACTCTGCTG 
TGCTCCCAGA GCTACTGCTG TCAGACTGAA CTGGAAGGGG AAGATGTGGG AGCCTGCACA 
GCTCACACCT ACTCCTGTGG TTCTGGGGTG GGCATCTTCC TCAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC 
TATGGGGAAA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAAAGAGCGA 
TTTAAGAAGA TTCAGAAGCT TTGGCACCAG CACAGTATCA CGGAGGAAAT TGGACATGCA 
CAGGAAGCAA ATCAGACACT GGTTGGCATC GACTGGCAAC ATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005627359.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627416.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

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CloneID	OCe15270
Clone ID Related Accession (Same CDS sequence)	XM_005627359.2 , XM_005627359.3
Accession Version	XM_005627359.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGGT TAAATATTTC AGACCAACCA 
AAACTGACTC AGTGGATTAG AACAATCTCT CAGCAAATAA AAGCATTACA GGTTCTTAGG 
AAAGAAGAAA GTACTCCTAA TACTGCTTCT TCAAAGAATT TAGAGAATAT GGATGAATTG 
CAGTTCCCTG AAGGGTTTAG GCCTGATTTT CATCCTAAGA ACCCTTATTC TGAGAGCATA 
AAAGAAATGC TAACAACATT TGGAACTGCT ACTTACAAGG TGGGACTAAA GGTTCATCCC 
AATGAGGAGG ATCCCCGTGT ACCCATAATG TGTTGGGGTA GTTGTGCATA CACCATCCAA 
AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTAT TTGGTCCTTT GCCTTGCAGA 
CTGGATGACT GTCTTAGGTC ATTAACAAGA TTTGCTGCAG CACATTGGAC AGTGGCATCA 
CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC AGATGACAAC 
CATGGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTG 
CTCGCTTTCC CTGCATTGCA GTGTCAGGAT TTTTCTGGGA TCAGCCTTGG CACTGGAGAT 
CTTCACATTT TCCATCTGGT TACTATGGCA CACATCGTAC AGATCTTACT TACCTCGTGT 
ACAGAAGAGA ATGGCATGGA TCAAGAAAAT CCTGCTGGTG AAGAAGAATT AGCAGTTCTT 
GCTTTGTATA AAACACTTCA CCAGTGTACA GGAAGTGCCT TGAAAGAAAT ACCATCTGGC 
TGGCATCTAT GGAGGAATGT CAGAAATGGA ATCATGCCTT TCCTTAAGTG TTGCGCTTTG 
TTTTTTCATT ACTTAAATGG AGTTCCTTCT CCACCTGAAC TTCAAGTTTC TGGAACAAGC 
CATTTTGAAC ATTTGTGTAA CTACCTTTCC CTGCCAAACA ACCTCATTTG CCTTTTTCAA 
GAAAATAATG AGATAACAAA ATTACTGATT GAAAGTTGGT GTCATAACAT TGAAGTTAAA 
AGATATCTTG AAGGTGAAAG AGATGCTATA AGCTATCCAA GAGAATCCAA CAAATTAATA 
GACCTTCCAG AGGATTATAG CAGCCTCATT AATCAAGCAT CTAATTTCTC ATGCCCCAAA 
TCAGGTGGTG ATAAGAGCAG AGCCCCCACT CTGTGCCTTG TGTGTGGAAC TCTGCTGTGC 
TCCCAGAGCT ACTGCTGTCA GACTGAACTG GAAGGGGAAG ATGTGGGAGC CTGCACAGCT 
CACACCTACT CCTGTGGTTC TGGGGTGGGC ATCTTCCTCA GAGTGCGGGA ATGTCAGGTG 
CTATTTTTAG CTGGCAAAAC CAAAGGCTGT TTTTATTCTC CTCCTTACCT TGATGACTAT 
GGGGAAACTG ACCAGGGACT CAGACGGGGA AATCCTTTAC ATTTGTGCAA AGAGCGATTT 
AAGAAGATTC AGAAGCTTTG GCACCAGCAC AGTATCACGG AGGAAATTGG ACATGCACAG 
GAAGCAAATC AGACACTGGT TGGCATCGAC TGGCAACATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627416.1
CDS	472..5736
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005627359.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627359.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGGT TAAATATTTC AGACCAACCA 
AAACTGACTC AGTGGATTAG AACAATCTCT CAGCAAATAA AAGCATTACA GGTTCTTAGG 
AAAGAAGAAA GTACTCCTAA TACTGCTTCT TCAAAGAATT TAGAGAATAT GGATGAATTG 
CAGTTCCCTG AAGGGTTTAG GCCTGATTTT CATCCTAAGA ACCCTTATTC TGAGAGCATA 
AAAGAAATGC TAACAACATT TGGAACTGCT ACTTACAAGG TGGGACTAAA GGTTCATCCC 
AATGAGGAGG ATCCCCGTGT ACCCATAATG TGTTGGGGTA GTTGTGCATA CACCATCCAA 
AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTAT TTGGTCCTTT GCCTTGCAGA 
CTGGATGACT GTCTTAGGTC ATTAACAAGA TTTGCTGCAG CACATTGGAC AGTGGCATCA 
CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC AGATGACAAC 
CATGGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTG 
CTCGCTTTCC CTGCATTGCA GTGTCAGGAT TTTTCTGGGA TCAGCCTTGG CACTGGAGAT 
CTTCACATTT TCCATCTGGT TACTATGGCA CACATCGTAC AGATCTTACT TACCTCGTGT 
ACAGAAGAGA ATGGCATGGA TCAAGAAAAT CCTGCTGGTG AAGAAGAATT AGCAGTTCTT 
GCTTTGTATA AAACACTTCA CCAGTGTACA GGAAGTGCCT TGAAAGAAAT ACCATCTGGC 
TGGCATCTAT GGAGGAATGT CAGAAATGGA ATCATGCCTT TCCTTAAGTG TTGCGCTTTG 
TTTTTTCATT ACTTAAATGG AGTTCCTTCT CCACCTGAAC TTCAAGTTTC TGGAACAAGC 
CATTTTGAAC ATTTGTGTAA CTACCTTTCC CTGCCAAACA ACCTCATTTG CCTTTTTCAA 
GAAAATAATG AGATAACAAA ATTACTGATT GAAAGTTGGT GTCATAACAT TGAAGTTAAA 
AGATATCTTG AAGGTGAAAG AGATGCTATA AGCTATCCAA GAGAATCCAA CAAATTAATA 
GACCTTCCAG AGGATTATAG CAGCCTCATT AATCAAGCAT CTAATTTCTC ATGCCCCAAA 
TCAGGTGGTG ATAAGAGCAG AGCCCCCACT CTGTGCCTTG TGTGTGGAAC TCTGCTGTGC 
TCCCAGAGCT ACTGCTGTCA GACTGAACTG GAAGGGGAAG ATGTGGGAGC CTGCACAGCT 
CACACCTACT CCTGTGGTTC TGGGGTGGGC ATCTTCCTCA GAGTGCGGGA ATGTCAGGTG 
CTATTTTTAG CTGGCAAAAC CAAAGGCTGT TTTTATTCTC CTCCTTACCT TGATGACTAT 
GGGGAAACTG ACCAGGGACT CAGACGGGGA AATCCTTTAC ATTTGTGCAA AGAGCGATTT 
AAGAAGATTC AGAAGCTTTG GCACCAGCAC AGTATCACGG AGGAAATTGG ACATGCACAG 
GAAGCAAATC AGACACTGGT TGGCATCGAC TGGCAACATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005627359.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627416.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

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CloneID	OCe15270
Clone ID Related Accession (Same CDS sequence)	XM_005627359.2 , XM_005627359.3
Accession Version	XM_005627359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGGT TAAATATTTC AGACCAACCA 
AAACTGACTC AGTGGATTAG AACAATCTCT CAGCAAATAA AAGCATTACA GGTTCTTAGG 
AAAGAAGAAA GTACTCCTAA TACTGCTTCT TCAAAGAATT TAGAGAATAT GGATGAATTG 
CAGTTCCCTG AAGGGTTTAG GCCTGATTTT CATCCTAAGA ACCCTTATTC TGAGAGCATA 
AAAGAAATGC TAACAACATT TGGAACTGCT ACTTACAAGG TGGGACTAAA GGTTCATCCC 
AATGAGGAGG ATCCCCGTGT ACCCATAATG TGTTGGGGTA GTTGTGCATA CACCATCCAA 
AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTAT TTGGTCCTTT GCCTTGCAGA 
CTGGATGACT GTCTTAGGTC ATTAACAAGA TTTGCTGCAG CACATTGGAC AGTGGCATCA 
CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC AGATGACAAC 
CATGGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTG 
CTCGCTTTCC CTGCATTGCA GTGTCAGGAT TTTTCTGGGA TCAGCCTTGG CACTGGAGAT 
CTTCACATTT TCCATCTGGT TACTATGGCA CACATCGTAC AGATCTTACT TACCTCGTGT 
ACAGAAGAGA ATGGCATGGA TCAAGAAAAT CCTGCTGGTG AAGAAGAATT AGCAGTTCTT 
GCTTTGTATA AAACACTTCA CCAGTGTACA GGAAGTGCCT TGAAAGAAAT ACCATCTGGC 
TGGCATCTAT GGAGGAATGT CAGAAATGGA ATCATGCCTT TCCTTAAGTG TTGCGCTTTG 
TTTTTTCATT ACTTAAATGG AGTTCCTTCT CCACCTGAAC TTCAAGTTTC TGGAACAAGC 
CATTTTGAAC ATTTGTGTAA CTACCTTTCC CTGCCAAACA ACCTCATTTG CCTTTTTCAA 
GAAAATAATG AGATAACAAA ATTACTGATT GAAAGTTGGT GTCATAACAT TGAAGTTAAA 
AGATATCTTG AAGGTGAAAG AGATGCTATA AGCTATCCAA GAGAATCCAA CAAATTAATA 
GACCTTCCAG AGGATTATAG CAGCCTCATT AATCAAGCAT CTAATTTCTC ATGCCCCAAA 
TCAGGTGGTG ATAAGAGCAG AGCCCCCACT CTGTGCCTTG TGTGTGGAAC TCTGCTGTGC 
TCCCAGAGCT ACTGCTGTCA GACTGAACTG GAAGGGGAAG ATGTGGGAGC CTGCACAGCT 
CACACCTACT CCTGTGGTTC TGGGGTGGGC ATCTTCCTCA GAGTGCGGGA ATGTCAGGTG 
CTATTTTTAG CTGGCAAAAC CAAAGGCTGT TTTTATTCTC CTCCTTACCT TGATGACTAT 
GGGGAAACTG ACCAGGGACT CAGACGGGGA AATCCTTTAC ATTTGTGCAA AGAGCGATTT 
AAGAAGATTC AGAAGCTTTG GCACCAGCAC AGTATCACGG AGGAAATTGG ACATGCACAG 
GAAGCAAATC AGACACTGGT TGGCATCGAC TGGCAACATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627416.1
CDS	61..5325
Translation	MASELEPEVQAIDRSLLECSAEETAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAIMFRYAVEILTWEKESELPADLEIVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGNVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAIRFAKNYERLQSDYVTDDHDREFSIADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLIFDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMIVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTVLQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLDNVTEEHVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPYLDVHKDMIRWILKTFNAIKKIRESSSTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDNSPMVSNMALTALGPAQTQVPEQRQFVTCILCQEEQEVKVESKAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNRLNISDQPKLTQWIRTISQQIKALQVLRKEESTPNTASSKNLENMDELQFPEGFRPDFHPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASLSVVQGHFCKLFASLVPDDNHGDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPAGEEELAVLALYKTLHQCTGSALKEIPSGWHLWRNVRNGIMPFLKCCALFFHYLNGVPSPPELQVSGTSHFEHLCNYLSLPNNLICLFQENNEITKLLIESWCHNIEVKRYLEGERDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005627359.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627359.3

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC CGAGGTGCAG GCCATCGACC GGAGTTTGCT GGAATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAG 
GAGGACATGC TGGCACAGCA TGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA 
GATCCCGCAT TTGGATTTCC AAAACTTGAA CAAGCAAATA AACCTTCTCA TCTTTGTGGT 
CGTGTTTTTA AAGTAGGAGA GCCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT 
TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCATCG ATATAGGATG 
ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT 
CCTTACTGTC AAAAACATGA ACTTAACACT TCTGAAATTG AGGAAGAAGA GGATCCTCTT 
GTCCATTTAT CAGAGGATGT GATAGCGAGA ACCTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTGG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCAGCAGA TTTAGAGATA 
GTAGAGAAGA GTGATACCTA CTATTGCATG CTGTTTAATG ATGAGGTGCA TACTTATGAA 
CAAGTTATTT ATACCCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTT 
GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGATTT CCAGTATTGT 
GAACAAGCAA AATCAGTAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT 
CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAACT TTTATCTTGG 
CTGGGAAATG TTATTGGATA TTCAGATGGG CTTCGCCGAA TTTTATGTCA AGTTGGTTTA 
CAAGAAGGGC CAGATGGCGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAATGATTCC 
AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TTATGAGCAG TCTACTTATG 
GATTTGAAAT ATAAGAAGTT GTTTGCTATT CGATTTGCAA AGAACTATGA GCGTTTGCAG 
AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACCT CTCGGTTCAG 
ATATTCACGG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATC 
ATCATTAAGA CTTTTATGGA TCATTTGAGA CATCGAGATG CCCAGGGCAG ATTTCAGTTT 
GAACGATATA CTGCTTTACA AGCCTTCAAG TTTAGGAGAG TTCAGAGCCT TATTTTTGAT 
CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGATCTGAG GCAGAAGTTC 
CTAGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT 
ACACGTCAAG TAGGACAGCA TATTGAAATG GAACCAGAGT GGGAGGCAGC CTTCACACTA 
CAAATGAAAT TAACACATGT CATTTCAATG ATGCAGGACT GGTGTGCTTT AGATGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA TGCAGTGTCA TGGTGGTTTC 
ACTGATGGTG AACAACCAAT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT 
TTACATGTAT TATTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC 
CAAGTACATG CAGGAATGTG GAGGAGAAAT GGCTTCTCTC TAGTAAACCA GATTTATTAC 
TACCATAATG TGAAATGCAG ACGTGAAATG TTTGACAAGG ATATAGTAAT GCTTCAGACA 
GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTCAGCCG CTTTGAACTT 
TATCAGATTT TCAGTACTCC AGACTATGGA AAAAGATTTA GCTCTGAGAT TACCCATAAG 
GATGTAGTTC AGCAAAACAA TACTCTAATA GAAGAAATGC TATACCTCAT TATAATGATT 
GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTAAATG CTACAGATGA AATTAAGCGA 
GAGATTATCC ATCAATTAAG CATCAAACCT ATGGCTCATA GTGAATTGGT GAAGTCTTTA 
CCTGAAGACG AGAACAAGGA GACTGGCATG GAGAGCGTAA TTGAAGCAGT TGCCCATTTC 
AAAAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG 
TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAA 
CGGAAATTGA AAAGACAAAA CAGGGAAGAT ACAGCACTTC CACCTCCAGT TTTGCCTCCT 
TTCTGCCCTC TATTTGCAAG TCTGGTAAAC ATTTTGCAGT CAGATGTGAT GTTGTGCATC 
ATGAGGACAG TACTGCAATG GGCTGTAGAA CATAACGGAT ATGCGTGGTC TGAGTCCATG 
CTCCAAAGGG TGTTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAC 
AATGTCACAG AAGAGCATGT AGTAACATTT ACCTTCACTC AGAAGATATC AAAACCTGGT 
GAAGCACCAA ACAACTCCCC TAGCATATTA GCTATGCTGG AAACACTACA GAATGCTCCC 
TACCTAGATG TCCACAAAGA TATGATTAGA TGGATATTAA AGACTTTTAA TGCTATTAAG 
AAGATAAGAG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CTGAAGGAAC CATAATGGAA 
GAGAGTTCAA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGACTG 
CGCAGAGAAA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC 
AAAGAACTCT TTCAGCAGAC ATTAGAACTA GATGCCTCTA CTTCTGCTGT TCTTGATAAT 
AGCCCTATGG TTTCAAATAT GGCACTTACA GCTTTGGGGC CAGCACAAAC TCAGGTCCCT 
GAACAGAGAC AGTTTGTTAC CTGTATATTG TGTCAAGAGG AGCAAGAGGT GAAGGTGGAG 
AGCAAGGCAA TGGTTCTTGC AGCATTTGTT CAAAGATCAA CTGTATTATC AAAAAATAGG 
AGTAAATTCA TTCAGGATCC AGAAAAATAT GATCCATTAT TCATGCACCC TGATCTGTCT 
TGCGGAACAC ACACTGGTAG CTGTGGGCAC ATTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTGCGCTT ACATACCAGC 
TATGATGTGG AAAACGGAGA ATTCCTTTGT CCCCTCTGTG AGTGCTTGAG TAATACTGTA 
ATTCCTCTGC TGCTTCCTCC ACGAAATATT TTTAACAGGT TAAATATTTC AGACCAACCA 
AAACTGACTC AGTGGATTAG AACAATCTCT CAGCAAATAA AAGCATTACA GGTTCTTAGG 
AAAGAAGAAA GTACTCCTAA TACTGCTTCT TCAAAGAATT TAGAGAATAT GGATGAATTG 
CAGTTCCCTG AAGGGTTTAG GCCTGATTTT CATCCTAAGA ACCCTTATTC TGAGAGCATA 
AAAGAAATGC TAACAACATT TGGAACTGCT ACTTACAAGG TGGGACTAAA GGTTCATCCC 
AATGAGGAGG ATCCCCGTGT ACCCATAATG TGTTGGGGTA GTTGTGCATA CACCATCCAA 
AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTAT TTGGTCCTTT GCCTTGCAGA 
CTGGATGACT GTCTTAGGTC ATTAACAAGA TTTGCTGCAG CACATTGGAC AGTGGCATCA 
CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC AGATGACAAC 
CATGGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTG 
CTCGCTTTCC CTGCATTGCA GTGTCAGGAT TTTTCTGGGA TCAGCCTTGG CACTGGAGAT 
CTTCACATTT TCCATCTGGT TACTATGGCA CACATCGTAC AGATCTTACT TACCTCGTGT 
ACAGAAGAGA ATGGCATGGA TCAAGAAAAT CCTGCTGGTG AAGAAGAATT AGCAGTTCTT 
GCTTTGTATA AAACACTTCA CCAGTGTACA GGAAGTGCCT TGAAAGAAAT ACCATCTGGC 
TGGCATCTAT GGAGGAATGT CAGAAATGGA ATCATGCCTT TCCTTAAGTG TTGCGCTTTG 
TTTTTTCATT ACTTAAATGG AGTTCCTTCT CCACCTGAAC TTCAAGTTTC TGGAACAAGC 
CATTTTGAAC ATTTGTGTAA CTACCTTTCC CTGCCAAACA ACCTCATTTG CCTTTTTCAA 
GAAAATAATG AGATAACAAA ATTACTGATT GAAAGTTGGT GTCATAACAT TGAAGTTAAA 
AGATATCTTG AAGGTGAAAG AGATGCTATA AGCTATCCAA GAGAATCCAA CAAATTAATA 
GACCTTCCAG AGGATTATAG CAGCCTCATT AATCAAGCAT CTAATTTCTC ATGCCCCAAA 
TCAGGTGGTG ATAAGAGCAG AGCCCCCACT CTGTGCCTTG TGTGTGGAAC TCTGCTGTGC 
TCCCAGAGCT ACTGCTGTCA GACTGAACTG GAAGGGGAAG ATGTGGGAGC CTGCACAGCT 
CACACCTACT CCTGTGGTTC TGGGGTGGGC ATCTTCCTCA GAGTGCGGGA ATGTCAGGTG 
CTATTTTTAG CTGGCAAAAC CAAAGGCTGT TTTTATTCTC CTCCTTACCT TGATGACTAT 
GGGGAAACTG ACCAGGGACT CAGACGGGGA AATCCTTTAC ATTTGTGCAA AGAGCGATTT 
AAGAAGATTC AGAAGCTTTG GCACCAGCAC AGTATCACGG AGGAAATTGG ACATGCACAG 
GAAGCAAATC AGACACTGGT TGGCATCGAC TGGCAACATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	UBR2
Entrez Gene ID	474903
Full Name	ubiquitin protein ligase E3 component n-recognin 2
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 2 Names E3 ubiquitin-protein ligase UBR2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Rattus norvegicus (Norway rat)	Ubr2	NP_001171542.1
Gallus gallus (chicken)	UBR2	XP_004935398.1
Drosophila melanogaster (fruit fly)	CG9086	NP_001245723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000171	XP_310967.5
Caenorhabditis elegans (roundworm)	ubr-1	NP_491231.2
Xenopus tropicalis (tropical clawed frog)	ubr2	XP_002934736.1
	UBR2	XP_001088074.2
Bos taurus (cattle)	UBR2	NP_001179567.1
Danio rerio (zebrafish)	ubr2	XP_005156554.1
Mus musculus (house mouse)	Ubr2	NP_001170845.1
Homo sapiens (human)	UBR2	NP_056070.1
Pan troglodytes (chimpanzee)	UBR2	XP_001134707.1
Canis lupus familiaris (dog)	UBR2	XP_005627416.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

UBR2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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