MUC1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MUC1
Entrez Gene ID	448784
Full Name	mucin 1, cell surface associated
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005622230.3	XP_005622287.1	mucin-1 isoform X2
XM_022420744.1	XP_022276452.1	mucin-1 isoform X1
XM_005622230.2	XP_005622287.1	mucin-1 isoform X1
NM_001194977.1	NP_001181906.1	mucin-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Determination of carcinoembryonic antigen and cancer antigen (CA 15-3) in bitches with tumours on mammary gland: preliminary report.
Valencakova-Agyagosova A, Frischova Z, Sevcikova Z, Hajurka J, Lepej J, Szakallova I, Kredatusova G, Nagy V, Ledecky V
Veterinary and comparative oncology12(3)205-14(2014 Sep)

CA 15-3 cell lines and tissue expression in canine mammary cancer and the correlation between serum levels and tumour histological grade.
Manuali E, De Giuseppe A, Feliziani F, Forti K, Casciari C, Marchesi MC, Pacifico E, Paw?owski KM, Majchrzak K, Kr?l M
BMC veterinary research886(2012 Jun)

Core-glycosylated mucin-like repeats from MUC1 are an apical targeting signal.
Kinlough CL, Poland PA, Gendler SJ, Mattila PE, Mo D, Weisz OA, Hughey RP
The Journal of biological chemistry286(45)39072-81(2011 Nov)

MUC1 traverses apical recycling endosomes along the biosynthetic pathway in polarized MDCK cells.
Mattila PE, Kinlough CL, Bruns JR, Weisz OA, Hughey RP
Biological chemistry390(7)551-6(2009 Jul)

Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus.
Ishiguro K, Baba E, Torii R, Tamada H, Kawate N, Hatoya S, Wijewardana V, Kumagai D, Sugiura K, Sawada T, Inaba T
Veterinary journal (London, England : 1997)173(2)325-32(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the core-glycosylated mucin-like repeats of MUC1 constitute an apical targeting signal.
Title: Core-glycosylated mucin-like repeats from MUC1 are an apical targeting signal.

MUC1 traffics to the apical surface via apical recycling endosomes in polarized renal epithelial cells
Title: MUC1 traverses apical recycling endosomes along the biosynthetic pathway in polarized MDCK cells.

The relation between adherence of Escherichia coli and expression of mucin-1 mRNA in the endometrium was studied in beagle bitches at different stages of the oestrous cycle and in those with pyometra is reported.
Title: Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus.

The following MUC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe33869	NM_001194977.1 Latest version!	Canis lupus familiaris mucin 1, cell surface associated (MUC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe08928	XM_022420744.1 Latest version!	Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe08929	XM_005622230.3 Latest version!	Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe08929	XM_005622230.2	Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MUC1 ( NM_001194977.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001181906.1 Canis lupus familiaris mucin 1, cell surface associated (MUC1), mRNA.

Price & Availability↑

CloneID	OCe33869
Clone ID Related Accession (Same CDS sequence)	NM_001194977.1
Accession Version	NM_001194977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Canis lupus familiaris(dog)
Product	mucin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03005338.1. On or before Aug 15, 2010 this sequence version replaced XM_537254.2, XM_849949.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGATTCCAA GCTTCCAGGC CCCTTTCTTC TTCCTGCTCC TGGTAACCCG AGAGCTTACA 
GCACAGGGAG GCAACTCATC CCTGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCCTC CTCATCCTGG GCCAGCAGCC ACACCACCAG GACTACCGCC 
AGTACCACTA ACCATAACAT GGAACCTCCC ATCTCCTCCA ATCATAGCAC TTCTCCCCAG 
TCTATTAGGA TCTCCTTCTT CTTCCTGTCC TTTTCCATTT TGAACCTCCA GTTTAATTCT 
TCTCTGGAAA ATCCCAGCAG CAACTACTAC CAGGAGCTGC AGAGGAACAT TTCTGAATTG 
TTTTTGCAGA TTTATGGACA GGAATATTTC CTGGGCCTTT ATAACATCAG ATTCAGACCC 
GGATCTGTGG TGGTAGAATC AACTCTGGCC TTCCGAAATG GTGCCACTGA TGCCAACAAG 
GTGAAGACAC AGTTTGAAGA CAGCAAAGAA GGAGCCAGAT ATAACCTGAA CATCTCGAGT 
ATTAGTGTGC GAGATGTGTC CTTCCCTTCC TCTGCCCCGT TCAAGTCTGG GGTTCCTGGC 
TGGGGCATTG CCCTGCTGGT GCTGGTCTGT GTTCTGGTTG CACTCGCCAT CATCTATGTT 
GTTGCCCTGA CTGTGTGCCA GTGCCGCCGA AAGAACTGTG GGCAGCTGGA CATCTTTCCA 
ACCCGAGACG CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT 
GTGCCCCCTG GCGGTAATAG ACGCAGCCCC TATGAGGAGG TTTCTGCAGG CAATGGGGGC 
AGCAGCCTCT CTTACATGAA CCCGAACCCA GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181906.1
CDS	37..1650
Translation	MIPSFQAPFFFLLLVTRELTAQGGNSSLTSSPASSSSSTLRKHSSSSLTSSPASSSTTSLGKLSSSSLTSSPVSGSIITPAQHGTSSPTSSPASSSSSTLRKHSSSSLTSSPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSSSWASSHTTRTTASTTNHNMEPPISSNHSTSPQSIRISFFFLSFSILNLQFNSSLENPSSNYYQELQRNISELFLQIYGQEYFLGLYNIRFRPGSVVVESTLAFRNGATDANKVKTQFEDSKEGARYNLNISSISVRDVSFPSSAPFKSGVPGWGIALLVLVCVLVALAIIYVVALTVCQCRRKNCGQLDIFPTRDAYHPMSEYPTYHTHGRYVPPGGNRRSPYEEVSAGNGGSSLSYMNPNPAATSANL

Target ORF information:

RefSeq Version	NM_001194977.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mucin 1, cell surface associated (MUC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194977.1

ORF Insert Sequence:

ATGATTCCAA GCTTCCAGGC CCCTTTCTTC TTCCTGCTCC TGGTAACCCG AGAGCTTACA 
GCACAGGGAG GCAACTCATC CCTGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCCTC CTCATCCTGG GCCAGCAGCC ACACCACCAG GACTACCGCC 
AGTACCACTA ACCATAACAT GGAACCTCCC ATCTCCTCCA ATCATAGCAC TTCTCCCCAG 
TCTATTAGGA TCTCCTTCTT CTTCCTGTCC TTTTCCATTT TGAACCTCCA GTTTAATTCT 
TCTCTGGAAA ATCCCAGCAG CAACTACTAC CAGGAGCTGC AGAGGAACAT TTCTGAATTG 
TTTTTGCAGA TTTATGGACA GGAATATTTC CTGGGCCTTT ATAACATCAG ATTCAGACCC 
GGATCTGTGG TGGTAGAATC AACTCTGGCC TTCCGAAATG GTGCCACTGA TGCCAACAAG 
GTGAAGACAC AGTTTGAAGA CAGCAAAGAA GGAGCCAGAT ATAACCTGAA CATCTCGAGT 
ATTAGTGTGC GAGATGTGTC CTTCCCTTCC TCTGCCCCGT TCAAGTCTGG GGTTCCTGGC 
TGGGGCATTG CCCTGCTGGT GCTGGTCTGT GTTCTGGTTG CACTCGCCAT CATCTATGTT 
GTTGCCCTGA CTGTGTGCCA GTGCCGCCGA AAGAACTGTG GGCAGCTGGA CATCTTTCCA 
ACCCGAGACG CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT 
GTGCCCCCTG GCGGTAATAG ACGCAGCCCC TATGAGGAGG TTTCTGCAGG CAATGGGGGC 
AGCAGCCTCT CTTACATGAA CCCGAACCCA GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MUC1 ( XM_022420744.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276452.1 Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08928
Clone ID Related Accession (Same CDS sequence)	XM_022420744.1
Accession Version	XM_022420744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mucin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCCC AGGTACCCTC CAGGAGGGCC CAGGAATGGT TGGGGAGGAG GAGGAAGAAG 
TCGGAGTTCA CCACCACCAT GATTCCAAGC TTCCAGGCCC CTTTCTTCTT CCTGCTCCTG 
GTAACCCGAG AGCTTACAGC ACAGGGAGGC AACTCATCCC TGACCAGCAG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCGACCA CCAGCCTGGG AAAACTCAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCAG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCCCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCGG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCATCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAACCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAAACA CCAGCCTGGG AAAACTCAGC TCCTCATCCC CAACCAGCCG CCCAGCCCCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCGG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCATCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAACCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAAACA CCAGCCTGGG AAAACTCAGC TCCTCATCCC CAACCAGCCG CCCAGCCCCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCGG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCAG CCCAGCCCCA 
AGCTTGACCT CCACCTTGAG AAAGCATAGC TCTTCATCCT TGACCAGCAG CCCAGCCCCA 
AGCTTGACCT CCACCTTGAG AAAGCATAGC TCTTCATCCT TGACCAGCAG CCCAGCCTCA 
AGCTCCACCT CCTCATCCCC TACCGGCAGC CCTGTCTCAG GCTCCACCAC CACCCCAGCA 
CAGCATGGCA CCTCATCCTC CACCACCAGC CTAGCCTCAA GCTCCACCTC CACCTTGGGA 
AAACTCAGCT CCTCATCCTG GGCCAGCAGC CACACCACCA GGACTACCGC CAGTACCACT 
AACCATAACA TGGAACCTCC CATCTCCTCC AATCATAGCA CTTCTCCCCA GTCTATTAGG 
ATCTCCTTCT TCTTCCTGTC CTTTTCCATT TTGAACCTCC AGTTTAATTC TTCTCTGGAA 
AATCCCAGCA GCAACTACTA CCAGGAGCTG CAGAGGAACA TTTCTGAATT GTTTTTGCAG 
ATTTATGGAC AGGAATATTT CCTGGGCCTT TATAACATCA GATTCAGACC CGGATCTGTG 
GTGGTAGAAT CAACTCTGGC CTTCCGAAAT GGTGCCACTG ATGCCAACAA GGTGAAGACA 
CAGTTTGAAG ACAGCAAAGA AGGAGCCAGA TATAACCTGA ACATCTCGAG TATTAGTGTG 
CGAGATGTGT CCTTCCCTTC CTCTGCCCCG TTCAAGTCTG GGGTTCCTGG CTGGGGCATT 
GCCCTGCTGG TGCTGGTCTG TGTTCTGGTT GCACTCGCCA TCATCTATGT TGTTGCCCTG 
ACTGTGTGCC AGTGCCGCCG AAAGAACTGT GGGCAGCTGG ACATCTTTCC AACCCGAGAC 
GCCTACCATC CTATGAGCGA GTACCCCACC TACCACACCC ATGGGCGCTA TGTGCCCCCT 
GGCGGTAATA GACGCAGCCC CTATGAGGAG GTTTCTGCAG GCAATGGGGG CAGCAGCCTC 
TCTTACATGA ACCCGAACCC AGCAGCCACT TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276452.1
CDS	571..2895
Translation	MGAQVPSRRAQEWLGRRRKKSEFTTTMIPSFQAPFFFLLLVTRELTAQGGNSSLTSSPASSSSSTLRKHSSSSLTSSPASSSTTSLGKLSSSSLTSSPVSGSIITPAQHGTSSPTSSPASSSSSTLRKHSSSSLTSSPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSSPAPSLTSTLRKHSSSSLTSSPAPSLTSTLRKHSSSSLTSSPASSSTSSSPTGSPVSGSTTTPAQHGTSSSTTSLASSSTSTLGKLSSSSWASSHTTRTTASTTNHNMEPPISSNHSTSPQSIRISFFFLSFSILNLQFNSSLENPSSNYYQELQRNISELFLQIYGQEYFLGLYNIRFRPGSVVVESTLAFRNGATDANKVKTQFEDSKEGARYNLNISSISVRDVSFPSSAPFKSGVPGWGIALLVLVCVLVALAIIYVVALTVCQCRRKNCGQLDIFPTRDAYHPMSEYPTYHTHGRYVPPGGNRRSPYEEVSAGNGGSSLSYMNPNPAATSANL

Target ORF information:

RefSeq Version	XM_022420744.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420744.1

ORF Insert Sequence:

ATGGGAGCCC AGGTACCCTC CAGGAGGGCC CAGGAATGGT TGGGGAGGAG GAGGAAGAAG 
TCGGAGTTCA CCACCACCAT GATTCCAAGC TTCCAGGCCC CTTTCTTCTT CCTGCTCCTG 
GTAACCCGAG AGCTTACAGC ACAGGGAGGC AACTCATCCC TGACCAGCAG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCGACCA CCAGCCTGGG AAAACTCAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCAG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCCCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCGG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCATCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAACCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAAACA CCAGCCTGGG AAAACTCAGC TCCTCATCCC CAACCAGCCG CCCAGCCCCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCGG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCATCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAACCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCAAACA CCAGCCTGGG AAAACTCAGC TCCTCATCCC CAACCAGCCG CCCAGCCCCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCGG CCCAGCCTCA 
AGCTCGTCCT CCACCTTGAG AAAGCATAGC TCCTCGTCCT TGACCAGCAG CCCAGCCTCA 
AGCTCGACCA CCAGCCTGGG AAAACACAGC TCCTCATCCT TGACCAGCAG CCCTGTCTCA 
GGCTCGATAA TCACCCCAGC ACAGCATGGC ACCTCATCCC CGACCAGCAG CCCAGCCCCA 
AGCTTGACCT CCACCTTGAG AAAGCATAGC TCTTCATCCT TGACCAGCAG CCCAGCCCCA 
AGCTTGACCT CCACCTTGAG AAAGCATAGC TCTTCATCCT TGACCAGCAG CCCAGCCTCA 
AGCTCCACCT CCTCATCCCC TACCGGCAGC CCTGTCTCAG GCTCCACCAC CACCCCAGCA 
CAGCATGGCA CCTCATCCTC CACCACCAGC CTAGCCTCAA GCTCCACCTC CACCTTGGGA 
AAACTCAGCT CCTCATCCTG GGCCAGCAGC CACACCACCA GGACTACCGC CAGTACCACT 
AACCATAACA TGGAACCTCC CATCTCCTCC AATCATAGCA CTTCTCCCCA GTCTATTAGG 
ATCTCCTTCT TCTTCCTGTC CTTTTCCATT TTGAACCTCC AGTTTAATTC TTCTCTGGAA 
AATCCCAGCA GCAACTACTA CCAGGAGCTG CAGAGGAACA TTTCTGAATT GTTTTTGCAG 
ATTTATGGAC AGGAATATTT CCTGGGCCTT TATAACATCA GATTCAGACC CGGATCTGTG 
GTGGTAGAAT CAACTCTGGC CTTCCGAAAT GGTGCCACTG ATGCCAACAA GGTGAAGACA 
CAGTTTGAAG ACAGCAAAGA AGGAGCCAGA TATAACCTGA ACATCTCGAG TATTAGTGTG 
CGAGATGTGT CCTTCCCTTC CTCTGCCCCG TTCAAGTCTG GGGTTCCTGG CTGGGGCATT 
GCCCTGCTGG TGCTGGTCTG TGTTCTGGTT GCACTCGCCA TCATCTATGT TGTTGCCCTG 
ACTGTGTGCC AGTGCCGCCG AAAGAACTGT GGGCAGCTGG ACATCTTTCC AACCCGAGAC 
GCCTACCATC CTATGAGCGA GTACCCCACC TACCACACCC ATGGGCGCTA TGTGCCCCCT 
GGCGGTAATA GACGCAGCCC CTATGAGGAG GTTTCTGCAG GCAATGGGGG CAGCAGCCTC 
TCTTACATGA ACCCGAACCC AGCAGCCACT TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MUC1 ( XM_005622230.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622287.1 Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08929
Clone ID Related Accession (Same CDS sequence)	XM_005622230.3 , XM_005622230.2
Accession Version	XM_005622230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mucin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAA GCTTCCAGGC CCCTTTCTTC TTCCTGCTCC TGGTAACCCG AGAGCTTACA 
GCACAGGGAG GCAACTCATC CCTGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCCAC CTCCTCATCC 
CCTACCGGCA GCCCTGTCTC AGGCTCCACC ACCACCCCAG CACAGCATGG CACCTCATCC 
TCCACCACCA GCCTAGCCTC AAGCTCCACC TCCACCTTGG GAAAACTCAG CTCCTCATCC 
TGGGCCAGCA GCCACACCAC CAGGACTACC GCCAGTACCA CTAACCATAA CATGGAACCT 
CCCATCTCCT CCAATCATAG CACTTCTCCC CAGTCTATTA GGATCTCCTT CTTCTTCCTG 
TCCTTTTCCA TTTTGAACCT CCAGTTTAAT TCTTCTCTGG AAAATCCCAG CAGCAACTAC 
TACCAGGAGC TGCAGAGGAA CATTTCTGAA TTGTTTTTGC AGATTTATGG ACAGGAATAT 
TTCCTGGGCC TTTATAACAT CAGATTCAGA CCCGGATCTG TGGTGGTAGA ATCAACTCTG 
GCCTTCCGAA ATGGTGCCAC TGATGCCAAC AAGGTGAAGA CACAGTTTGA AGACAGCAAA 
GAAGGAGCCA GATATAACCT GAACATCTCG AGTATTAGTG TGCGAGATGT GTCCTTCCCT 
TCCTCTGCCC CGTTCAAGTC TGGGGTTCCT GGCTGGGGCA TTGCCCTGCT GGTGCTGGTC 
TGTGTTCTGG TTGCACTCGC CATCATCTAT GTTGTTGCCC TGACTGTGTG CCAGTGCCGC 
CGAAAGAACT GTGGGCAGCT GGACATCTTT CCAACCCGAG ACGCCTACCA TCCTATGAGC 
GAGTACCCCA CCTACCACAC CCATGGGCGC TATGTGCCCC CTGGCGGTAA TAGACGCAGC 
CCCTATGAGG AGGTTTCTGC AGGCAATGGG GGCAGCAGCC TCTCTTACAT GAACCCGAAC 
CCAGCAGCCA CTTCTGCCAA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622287.1
CDS	343..2589
Translation	MIPSFQAPFFFLLLVTRELTAQGGNSSLTSSPASSSSSTLRKHSSSSLTSSPASSSTTSLGKLSSSSLTSSPVSGSIITPAQHGTSSPTSSPASSSSSTLRKHSSSSLTSSPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSSPAPSLTSTLRKHSSSSLTSSPAPSLTSTLRKHSSSSLTSSPASSSTSSSPTGSPVSGSTTTPAQHGTSSSTTSLASSSTSTLGKLSSSSWASSHTTRTTASTTNHNMEPPISSNHSTSPQSIRISFFFLSFSILNLQFNSSLENPSSNYYQELQRNISELFLQIYGQEYFLGLYNIRFRPGSVVVESTLAFRNGATDANKVKTQFEDSKEGARYNLNISSISVRDVSFPSSAPFKSGVPGWGIALLVLVCVLVALAIIYVVALTVCQCRRKNCGQLDIFPTRDAYHPMSEYPTYHTHGRYVPPGGNRRSPYEEVSAGNGGSSLSYMNPNPAATSANL

Target ORF information:

RefSeq Version	XM_005622230.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622230.3

ORF Insert Sequence:

ATGATTCCAA GCTTCCAGGC CCCTTTCTTC TTCCTGCTCC TGGTAACCCG AGAGCTTACA 
GCACAGGGAG GCAACTCATC CCTGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCCAC CTCCTCATCC 
CCTACCGGCA GCCCTGTCTC AGGCTCCACC ACCACCCCAG CACAGCATGG CACCTCATCC 
TCCACCACCA GCCTAGCCTC AAGCTCCACC TCCACCTTGG GAAAACTCAG CTCCTCATCC 
TGGGCCAGCA GCCACACCAC CAGGACTACC GCCAGTACCA CTAACCATAA CATGGAACCT 
CCCATCTCCT CCAATCATAG CACTTCTCCC CAGTCTATTA GGATCTCCTT CTTCTTCCTG 
TCCTTTTCCA TTTTGAACCT CCAGTTTAAT TCTTCTCTGG AAAATCCCAG CAGCAACTAC 
TACCAGGAGC TGCAGAGGAA CATTTCTGAA TTGTTTTTGC AGATTTATGG ACAGGAATAT 
TTCCTGGGCC TTTATAACAT CAGATTCAGA CCCGGATCTG TGGTGGTAGA ATCAACTCTG 
GCCTTCCGAA ATGGTGCCAC TGATGCCAAC AAGGTGAAGA CACAGTTTGA AGACAGCAAA 
GAAGGAGCCA GATATAACCT GAACATCTCG AGTATTAGTG TGCGAGATGT GTCCTTCCCT 
TCCTCTGCCC CGTTCAAGTC TGGGGTTCCT GGCTGGGGCA TTGCCCTGCT GGTGCTGGTC 
TGTGTTCTGG TTGCACTCGC CATCATCTAT GTTGTTGCCC TGACTGTGTG CCAGTGCCGC 
CGAAAGAACT GTGGGCAGCT GGACATCTTT CCAACCCGAG ACGCCTACCA TCCTATGAGC 
GAGTACCCCA CCTACCACAC CCATGGGCGC TATGTGCCCC CTGGCGGTAA TAGACGCAGC 
CCCTATGAGG AGGTTTCTGC AGGCAATGGG GGCAGCAGCC TCTCTTACAT GAACCCGAAC 
CCAGCAGCCA CTTCTGCCAA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MUC1 ( XM_005622230.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622287.1 Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X1, mRNA.

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CloneID	OCe08929
Clone ID Related Accession (Same CDS sequence)	XM_005622230.3 , XM_005622230.2
Accession Version	XM_005622230.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mucin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAA GCTTCCAGGC CCCTTTCTTC TTCCTGCTCC TGGTAACCCG AGAGCTTACA 
GCACAGGGAG GCAACTCATC CCTGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCCAC CTCCTCATCC 
CCTACCGGCA GCCCTGTCTC AGGCTCCACC ACCACCCCAG CACAGCATGG CACCTCATCC 
TCCACCACCA GCCTAGCCTC AAGCTCCACC TCCACCTTGG GAAAACTCAG CTCCTCATCC 
TGGGCCAGCA GCCACACCAC CAGGACTACC GCCAGTACCA CTAACCATAA CATGGAACCT 
CCCATCTCCT CCAATCATAG CACTTCTCCC CAGTCTATTA GGATCTCCTT CTTCTTCCTG 
TCCTTTTCCA TTTTGAACCT CCAGTTTAAT TCTTCTCTGG AAAATCCCAG CAGCAACTAC 
TACCAGGAGC TGCAGAGGAA CATTTCTGAA TTGTTTTTGC AGATTTATGG ACAGGAATAT 
TTCCTGGGCC TTTATAACAT CAGATTCAGA CCCGGATCTG TGGTGGTAGA ATCAACTCTG 
GCCTTCCGAA ATGGTGCCAC TGATGCCAAC AAGGTGAAGA CACAGTTTGA AGACAGCAAA 
GAAGGAGCCA GATATAACCT GAACATCTCG AGTATTAGTG TGCGAGATGT GTCCTTCCCT 
TCCTCTGCCC CGTTCAAGTC TGGGGTTCCT GGCTGGGGCA TTGCCCTGCT GGTGCTGGTC 
TGTGTTCTGG TTGCACTCGC CATCATCTAT GTTGTTGCCC TGACTGTGTG CCAGTGCCGC 
CGAAAGAACT GTGGGCAGCT GGACATCTTT CCAACCCGAG ACGCCTACCA TCCTATGAGC 
GAGTACCCCA CCTACCACAC CCATGGGCGC TATGTGCCCC CTGGCGGTAA TAGACGCAGC 
CCCTATGAGG AGGTTTCTGC AGGCAATGGG GGCAGCAGCC TCTCTTACAT GAACCCGAAC 
CCAGCAGCCA CTTCTGCCAA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622287.1
CDS	379..2625
Translation	MIPSFQAPFFFLLLVTRELTAQGGNSSLTSSPASSSSSTLRKHSSSSLTSSPASSSTTSLGKLSSSSLTSSPVSGSIITPAQHGTSSPTSSPASSSSSTLRKHSSSSLTSSPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTSTLRKHSSSSLTSSPASSSNTSLGKLSSSSPTSRPAPSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSGPASSSSSTLRKHSSSSLTSSPASSSTTSLGKHSSSSLTSSPVSGSIITPAQHGTSSPTSSPAPSLTSTLRKHSSSSLTSSPAPSLTSTLRKHSSSSLTSSPASSSTSSSPTGSPVSGSTTTPAQHGTSSSTTSLASSSTSTLGKLSSSSWASSHTTRTTASTTNHNMEPPISSNHSTSPQSIRISFFFLSFSILNLQFNSSLENPSSNYYQELQRNISELFLQIYGQEYFLGLYNIRFRPGSVVVESTLAFRNGATDANKVKTQFEDSKEGARYNLNISSISVRDVSFPSSAPFKSGVPGWGIALLVLVCVLVALAIIYVVALTVCQCRRKNCGQLDIFPTRDAYHPMSEYPTYHTHGRYVPPGGNRRSPYEEVSAGNGGSSLSYMNPNPAATSANL

Target ORF information:

RefSeq Version	XM_005622230.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mucin 1, cell surface associated (MUC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622230.2

ORF Insert Sequence:

ATGATTCCAA GCTTCCAGGC CCCTTTCTTC TTCCTGCTCC TGGTAACCCG AGAGCTTACA 
GCACAGGGAG GCAACTCATC CCTGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCAAC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCAAA CACCAGCCTG 
GGAAAACTCA GCTCCTCATC CCCAACCAGC CGCCCAGCCC CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC GGCCCAGCCT CAAGCTCGTC CTCCACCTTG 
AGAAAGCATA GCTCCTCGTC CTTGACCAGC AGCCCAGCCT CAAGCTCGAC CACCAGCCTG 
GGAAAACACA GCTCCTCATC CTTGACCAGC AGCCCTGTCT CAGGCTCGAT AATCACCCCA 
GCACAGCATG GCACCTCATC CCCGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCC CAAGCTTGAC CTCCACCTTG 
AGAAAGCATA GCTCTTCATC CTTGACCAGC AGCCCAGCCT CAAGCTCCAC CTCCTCATCC 
CCTACCGGCA GCCCTGTCTC AGGCTCCACC ACCACCCCAG CACAGCATGG CACCTCATCC 
TCCACCACCA GCCTAGCCTC AAGCTCCACC TCCACCTTGG GAAAACTCAG CTCCTCATCC 
TGGGCCAGCA GCCACACCAC CAGGACTACC GCCAGTACCA CTAACCATAA CATGGAACCT 
CCCATCTCCT CCAATCATAG CACTTCTCCC CAGTCTATTA GGATCTCCTT CTTCTTCCTG 
TCCTTTTCCA TTTTGAACCT CCAGTTTAAT TCTTCTCTGG AAAATCCCAG CAGCAACTAC 
TACCAGGAGC TGCAGAGGAA CATTTCTGAA TTGTTTTTGC AGATTTATGG ACAGGAATAT 
TTCCTGGGCC TTTATAACAT CAGATTCAGA CCCGGATCTG TGGTGGTAGA ATCAACTCTG 
GCCTTCCGAA ATGGTGCCAC TGATGCCAAC AAGGTGAAGA CACAGTTTGA AGACAGCAAA 
GAAGGAGCCA GATATAACCT GAACATCTCG AGTATTAGTG TGCGAGATGT GTCCTTCCCT 
TCCTCTGCCC CGTTCAAGTC TGGGGTTCCT GGCTGGGGCA TTGCCCTGCT GGTGCTGGTC 
TGTGTTCTGG TTGCACTCGC CATCATCTAT GTTGTTGCCC TGACTGTGTG CCAGTGCCGC 
CGAAAGAACT GTGGGCAGCT GGACATCTTT CCAACCCGAG ACGCCTACCA TCCTATGAGC 
GAGTACCCCA CCTACCACAC CCATGGGCGC TATGTGCCCC CTGGCGGTAA TAGACGCAGC 
CCCTATGAGG AGGTTTCTGC AGGCAATGGG GGCAGCAGCC TCTCTTACAT GAACCCGAAC 
CCAGCAGCCA CTTCTGCCAA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Determination of carcinoembryonic antigen and cancer antigen (CA 15-3) in bitches with tumours on mammary gland: preliminary report.
Veterinary and comparative oncology12(3)205-14(2014 Sep)
Valencakova-Agyagosova A,Frischova Z,Sevcikova Z,Hajurka J,Lepej J,Szakallova I,Kredatusova G,Nagy V,Ledecky V

CA 15-3 cell lines and tissue expression in canine mammary cancer and the correlation between serum levels and tumour histological grade.
BMC veterinary research886(2012 Jun)
Manuali E,De Giuseppe A,Feliziani F,Forti K,Casciari C,Marchesi MC,Pacifico E,Paw?owski KM,Majchrzak K,Kr?l M

Core-glycosylated mucin-like repeats from MUC1 are an apical targeting signal.
The Journal of biological chemistry286(45)39072-81(2011 Nov)
Kinlough CL,Poland PA,Gendler SJ,Mattila PE,Mo D,Weisz OA,Hughey RP

MUC1 traverses apical recycling endosomes along the biosynthetic pathway in polarized MDCK cells.
Biological chemistry390(7)551-6(2009 Jul)
Mattila PE,Kinlough CL,Bruns JR,Weisz OA,Hughey RP

Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus.
Veterinary journal (London, England : 1997)173(2)325-32(2007 Mar)
Ishiguro K,Baba E,Torii R,Tamada H,Kawate N,Hatoya S,Wijewardana V,Kumagai D,Sugiura K,Sawada T,Inaba T

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MUC1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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