NFKB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFKB1
Entrez Gene ID	442859
Full Name	nuclear factor kappa B subunit 1
General protein information	Preferred Names nuclear factor kappa B subunit 1 Names nuclear factor NF-kappa-B p105 subunit nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003344.1	NP_001003344.1	nuclear factor NF-kappa-B p105 subunit

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFKB1	XP_001168718.1
Canis lupus familiaris (dog)	NFKB1	NP_001003344.1
Bos taurus (cattle)	NFKB1	NP_001069877.1
Rattus norvegicus (Norway rat)	Nfkb1	NP_001263640.1
Gallus gallus (chicken)	NFKB1	NP_990465.1
Mus musculus (house mouse)	Nfkb1	NP_032715.2
Homo sapiens (human)	NFKB1	NP_003989.2
Xenopus tropicalis (tropical clawed frog)	nfkb1	XP_002934805.2
	NFKB1	XP_002804199.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of MEK/ERK pathways through NF-?B activation is involved in interleukin-1?-induced cyclooxygenease-2 expression in canine dermal fibroblasts.
Tsuchiya H, Nakano R, Konno T, Okabayashi K, Narita T, Sugiya H
Veterinary immunology and immunopathology168(3-4)223-32(2015 Dec)

Short communication: molecular characterization of dog and cat p65 subunits of NF-kappaB.
Ishikawa S, Takemitsu H, Li G, Mori N, Yamamoto I, Arai T
Research in veterinary science99145-8(2015 Apr)

Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.
Pei Z, Wang J
The Journal of veterinary medical science77(2)139-45(2015 Feb)

Activation of nuclear factor-kappa B and cell adhesion molecule mRNA expression in duodenal mucosa of dogs with lymphocytic-plasmacytic enteritis.
Okanishi H, Kabeya H, Maruyama S, Kagawa Y, Watari T
Veterinary immunology and immunopathology154(3-4)145-52(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that stimulation of MEK/ERK signaling pathway by NF-kappaB activation regulates IL-1beta-induced COX-2 expression and subsequent prostaglandin E2 release in canine dermal fibroblasts.
Title: Activation of MEK/ERK pathways through NF-?B activation is involved in interleukin-1?-induced cyclooxygenease-2 expression in canine dermal fibroblasts.

The dog p65 and cat p65 subunits of NF-kappaB complementary DNA encoded 542 and 555 amino acids, respectively. The p65 messenger RNA is highly expressed in the dog stomach and cat heart and adipose tissue.
Title: Short communication: molecular characterization of dog and cat p65 subunits of NF-kappaB.

Non-cytotoxic levels of propofol pretreatment can down-regulate LPS-induced inflammatory responses in canine PBMCs, possibly by inhibiting the nuclear translocation of the NF-kappaB p65 protein.
Title: Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.

These results suggest that NFkappaB and CAMs may play important roles in the pathogenesis of canine lymphocytic-plasmacytic enteritis.
Title: Activation of nuclear factor-kappa B and cell adhesion molecule mRNA expression in duodenal mucosa of dogs with lymphocytic-plasmacytic enteritis.

These data suggest that NOD2 and NFkappaB play an important role in the pathogenesis of LPC.
Title: NOD2 mRNA expression and NFkappaB activation in dogs with lymphocytic plasmacytic colitis.

The following NFKB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFKB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34316	NM_001003344.1 Latest version!	Canis lupus familiaris nuclear factor kappa B subunit 1 (NFKB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFKB1 ( NM_001003344.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003344.1 Canis lupus familiaris nuclear factor kappa B subunit 1 (NFKB1), mRNA.

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CloneID	OCe34316
Clone ID Related Accession (Same CDS sequence)	NM_001003344.1
Accession Version	NM_001003344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Canis lupus familiaris(dog)
Product	nuclear factor NF-kappa-B p105 subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB183419.1. ##Evidence-Data-START## Transcript exon combination :: AB183419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAG ACGATACATA TTTGGGAGCG CATGAGCAAA TGTTTCACTT GGATCCTTTG 
ACTCATACAA TATTTAATCC AGAATTATTT CAACCAGAGA TGCCACTACC CACAGCAGAT 
GGTCCATATC TTCAAATATT AGAGCAACCT AAACAGAGAG GATTTCGTTT CCGTTATGTA 
TGTGAAGGCC CATCCCATGG TGGACTCCCT GGTGCATCTA GTGAAAAGAA CAAGAAGTCC 
TACCCCCAGG TCAAAATCTG CAACTATGTG GGACCTGCAA AGGTTATTGT TCAGTTGGTC 
ACAAATGGGA AAAACATCCA CCTGCATGCA CACAGCCTGG TGGGAAAACA CTGTGAGGAT 
GGGATCTGCA CTGTAACTGC TGGACCCAAG GACATGGTGG TCGGCTTTGC AAACCTGGGT 
ATACTTCATG TGACAAAGAA AAAAGTATTT GAAACACTGG AAGCACGAAT GACAGAGGCA 
TGTACGAAGG GCTACAATCC TGGCCTTTTG GTGCATCCGG ATCTCGCCTA TCTGCAAGCG 
GAAGGCGGAG GAGACCGGCA GCTTACAGAT CGGGAAAAGG AGATCATCCG CCAGGCAGCT 
CTTCAGCAGA CAAAGGAGAT GGACCTCAGT GTGGTGCGGC TCATGTTTAC AGCTTTTCTT 
CCAGATAGCA CCGGCAGCTT CACAAGGCGC CTGGAACCCG TAGTGTCAGA TGCCATCTAC 
GACAGCAAAG CCCCCAATGC GTCCAACTTG AAAATTGTAA GAATGGACAG GACAGCTGGA 
TGCGTAACAG GAGGGGAAGA AATTTATCTT CTCTGTGACA AGGTTCAGAA AGATGACATC 
CAGATTCGAT TTTATGAAGA GGAAGGAAAT GGTGGGATCT GGGGAGGATT TGGGGATTTT 
TCCCCCACAG ATGTTCATAG ACAATTTGCC ATTGTCTTCA AAACGCCAAA GTATAAAGAT 
GTCAACATCA CAAAACCAAC CTCTGTGTTC GTCCAGCTTC GGAGGAAATC TGATTTGGAA 
ACTAGTGAAC CCAAACCTTT TCTCTACTAC CCTGAAATCA AAGATAAAGA GGAAGTGCAG 
AGGAAACGGC AGAAGCTCAT GCCTAATTTC TCAGATAGTT TCGGTGGCGG TAGTGGAGCA 
GGAGCTGGAG GCGGAGGCAT GTTTGGCAGC GGTGGTGGAG GAGGCGGTGC TGGAAGTACG 
GGTCCAGGGT ATGGTTTCCC TCACTATGGA TTTCCTACAT ATGGTGGAAT TACTTTCCAT 
CCTGGAACCA CTAAATCTAA TGCCGGGATG AAAAATGGAA CCATAGACAC CCCATCTAAA 
AATGATTCTG AAGGTTGTGG CAAGAATGTG GACAGAGAGG CAGTAAACCT CTCTGGGAAA 
GTAACTGAAC CCACAGAACA AGATAAGGAG TCCAGCATGG GGGTTGATGA GGTTACTCTG 
ACATATACAG TGGGCATAAA GGAAGAGAAT TCTAGGTTTC AAGATAACCT CTTTCTAGAA 
AAGGCTATGC AGCTTGCCAA GCGGCATGCC AATGCCCTTT TTGACTATGC GGTGACAGGA 
GATGTGAAAA TGCTGCTGGC TGTCCAGCGC CATCTCACTG CAGTGCAGGA TGGGAATGGG 
GACAGTGTCT TACACTTAGC GATCATCCAT CTTCATGCTC AACTTGTGAG GGATCTACTA 
GAAGTCACAT CTGGTTTGAT TTCTGATGAC ATTATCAATA TGAGGAATGA TCTCTACCAG 
ACGCCCTTGC ACTTGGCAGT GATTACCAAG CAGGAGGCTG TGGTGGACGA CTTGCTGAGG 
GCCGGGGCCG ACCTGAGCCT TCTGGACCGC TTGGGTAACT CTGTTTTGCA CCTAGCTGCC 
AAAGAAGGAC AAGATAAGAT TCTCAGTATT TTACTCAAGC ACAAAAAGGC AGCACTACTT 
ATGGACCACC CTAACGGGGA AGGTCTAAAC GCCATTCACA TAGCCGTGAT GAGCAACAGC 
ATGCCGTGTC TGCTGCTGCT GGTGGCCGCC GGGGCCGACG TCAACGCCCA GGAGCGGAAG 
TCGGGGCGCA CGGCGCTTCA CCTGGCCGTG GAGCACGACA ACATCTCCCT GGCTGGCTGC 
CTGCTCCTGG AGGGTGATGC CCATGTAGAC AGTACGACCT ACGATGGAAC TACACCCCTG 
CACATAGCAG CCGGGAGAGG GTCCACCAGG CTGGCAGCTC TTCTCAAAGC AGCAGGTGCA 
GATCCTCTGG TGGAGAACTT TGAGCCTCTC TATGACCTGG ATGACTCTTG GGAAAAAGAT 
GGAGAGGATG AAGGAGTCGT GCCTGGAACC ACACCTCTAG ATATGGCCAC CAACTGGCAG 
GTGTTTGATA TACTAAATGG GAAACCATAT GAGCCAGAGT TTACATCTGA CGATTTACTT 
GCACAAGGAG ACATGAAACA GCTGACTGAG GATGCTAAAT TGCAGCTCTA CAAGTTGCTA 
GAGACCCCTG ATCCGGACAA AAACTGGGCT ACTCTGGCAC AGAAATTAGG TCAGGGGATA 
CTGAATAATG CCTTCCGGCT GAGTCCTGCT CCTTCTAAAA CTCTTATGGA TAACTACGAA 
GTCTCCGGGG GGACAATAAA AGAGCTGGTG GAGGCCCTGA GACAGATGGG CTATACCGAA 
GCCATCGAAG TGATCCAGGC GGCCTTCTGC GCTCCGGAAA CTGCGGCCCC CAGCCCAGGG 
AAGGGTGCAC CTCAGACCCT CTCGCTGCCC CTCTCATCTG CCTCCACGAG GTCGCCAGTA 
GACGAGGTCC GAGACGACAG CATCTGTGAC AGCGGCGTGG AGACCTCCTT CCGCAGGCTC 
AGCTTCACAG AGTCTCTGAC CAGTGGCAGC TCCTTGCTGA CTCTTAACAA AGCGCCCCAC 
GAGTACGGGC AGGAAGGACC GATAGAGGGC AAAATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003344.1
CDS	51..2969
Translation	MAEDDTYLGAHEQMFHLDPLTHTIFNPELFQPEMPLPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACTKGYNPGLLVHPDLAYLQAEGGGDRQLTDREKEIIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEGNGGIWGGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPTSVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGAGSTGPGYGFPHYGFPTYGGITFHPGTTKSNAGMKNGTIDTPSKNDSEGCGKNVDREAVNLSGKVTEPTEQDKESSMGVDEVTLTYTVGIKEENSRFQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDGNGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEAVVDDLLRAGADLSLLDRLGNSVLHLAAKEGQDKILSILLKHKKAALLMDHPNGEGLNAIHIAVMSNSMPCLLLLVAAGADVNAQERKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKDGEDEGVVPGTTPLDMATNWQVFDILNGKPYEPEFTSDDLLAQGDMKQLTEDAKLQLYKLLETPDPDKNWATLAQKLGQGILNNAFRLSPAPSKTLMDNYEVSGGTIKELVEALRQMGYTEAIEVIQAAFCAPETAAPSPGKGAPQTLSLPLSSASTRSPVDEVRDDSICDSGVETSFRRLSFTESLTSGSSLLTLNKAPHEYGQEGPIEGKI

Target ORF information:

RefSeq Version	NM_001003344.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear factor kappa B subunit 1 (NFKB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003344.1

ORF Insert Sequence:

ATGGCAGAAG ACGATACATA TTTGGGAGCG CATGAGCAAA TGTTTCACTT GGATCCTTTG 
ACTCATACAA TATTTAATCC AGAATTATTT CAACCAGAGA TGCCACTACC CACAGCAGAT 
GGTCCATATC TTCAAATATT AGAGCAACCT AAACAGAGAG GATTTCGTTT CCGTTATGTA 
TGTGAAGGCC CATCCCATGG TGGACTCCCT GGTGCATCTA GTGAAAAGAA CAAGAAGTCC 
TACCCCCAGG TCAAAATCTG CAACTATGTG GGACCTGCAA AGGTTATTGT TCAGTTGGTC 
ACAAATGGGA AAAACATCCA CCTGCATGCA CACAGCCTGG TGGGAAAACA CTGTGAGGAT 
GGGATCTGCA CTGTAACTGC TGGACCCAAG GACATGGTGG TCGGCTTTGC AAACCTGGGT 
ATACTTCATG TGACAAAGAA AAAAGTATTT GAAACACTGG AAGCACGAAT GACAGAGGCA 
TGTACGAAGG GCTACAATCC TGGCCTTTTG GTGCATCCGG ATCTCGCCTA TCTGCAAGCG 
GAAGGCGGAG GAGACCGGCA GCTTACAGAT CGGGAAAAGG AGATCATCCG CCAGGCAGCT 
CTTCAGCAGA CAAAGGAGAT GGACCTCAGT GTGGTGCGGC TCATGTTTAC AGCTTTTCTT 
CCAGATAGCA CCGGCAGCTT CACAAGGCGC CTGGAACCCG TAGTGTCAGA TGCCATCTAC 
GACAGCAAAG CCCCCAATGC GTCCAACTTG AAAATTGTAA GAATGGACAG GACAGCTGGA 
TGCGTAACAG GAGGGGAAGA AATTTATCTT CTCTGTGACA AGGTTCAGAA AGATGACATC 
CAGATTCGAT TTTATGAAGA GGAAGGAAAT GGTGGGATCT GGGGAGGATT TGGGGATTTT 
TCCCCCACAG ATGTTCATAG ACAATTTGCC ATTGTCTTCA AAACGCCAAA GTATAAAGAT 
GTCAACATCA CAAAACCAAC CTCTGTGTTC GTCCAGCTTC GGAGGAAATC TGATTTGGAA 
ACTAGTGAAC CCAAACCTTT TCTCTACTAC CCTGAAATCA AAGATAAAGA GGAAGTGCAG 
AGGAAACGGC AGAAGCTCAT GCCTAATTTC TCAGATAGTT TCGGTGGCGG TAGTGGAGCA 
GGAGCTGGAG GCGGAGGCAT GTTTGGCAGC GGTGGTGGAG GAGGCGGTGC TGGAAGTACG 
GGTCCAGGGT ATGGTTTCCC TCACTATGGA TTTCCTACAT ATGGTGGAAT TACTTTCCAT 
CCTGGAACCA CTAAATCTAA TGCCGGGATG AAAAATGGAA CCATAGACAC CCCATCTAAA 
AATGATTCTG AAGGTTGTGG CAAGAATGTG GACAGAGAGG CAGTAAACCT CTCTGGGAAA 
GTAACTGAAC CCACAGAACA AGATAAGGAG TCCAGCATGG GGGTTGATGA GGTTACTCTG 
ACATATACAG TGGGCATAAA GGAAGAGAAT TCTAGGTTTC AAGATAACCT CTTTCTAGAA 
AAGGCTATGC AGCTTGCCAA GCGGCATGCC AATGCCCTTT TTGACTATGC GGTGACAGGA 
GATGTGAAAA TGCTGCTGGC TGTCCAGCGC CATCTCACTG CAGTGCAGGA TGGGAATGGG 
GACAGTGTCT TACACTTAGC GATCATCCAT CTTCATGCTC AACTTGTGAG GGATCTACTA 
GAAGTCACAT CTGGTTTGAT TTCTGATGAC ATTATCAATA TGAGGAATGA TCTCTACCAG 
ACGCCCTTGC ACTTGGCAGT GATTACCAAG CAGGAGGCTG TGGTGGACGA CTTGCTGAGG 
GCCGGGGCCG ACCTGAGCCT TCTGGACCGC TTGGGTAACT CTGTTTTGCA CCTAGCTGCC 
AAAGAAGGAC AAGATAAGAT TCTCAGTATT TTACTCAAGC ACAAAAAGGC AGCACTACTT 
ATGGACCACC CTAACGGGGA AGGTCTAAAC GCCATTCACA TAGCCGTGAT GAGCAACAGC 
ATGCCGTGTC TGCTGCTGCT GGTGGCCGCC GGGGCCGACG TCAACGCCCA GGAGCGGAAG 
TCGGGGCGCA CGGCGCTTCA CCTGGCCGTG GAGCACGACA ACATCTCCCT GGCTGGCTGC 
CTGCTCCTGG AGGGTGATGC CCATGTAGAC AGTACGACCT ACGATGGAAC TACACCCCTG 
CACATAGCAG CCGGGAGAGG GTCCACCAGG CTGGCAGCTC TTCTCAAAGC AGCAGGTGCA 
GATCCTCTGG TGGAGAACTT TGAGCCTCTC TATGACCTGG ATGACTCTTG GGAAAAAGAT 
GGAGAGGATG AAGGAGTCGT GCCTGGAACC ACACCTCTAG ATATGGCCAC CAACTGGCAG 
GTGTTTGATA TACTAAATGG GAAACCATAT GAGCCAGAGT TTACATCTGA CGATTTACTT 
GCACAAGGAG ACATGAAACA GCTGACTGAG GATGCTAAAT TGCAGCTCTA CAAGTTGCTA 
GAGACCCCTG ATCCGGACAA AAACTGGGCT ACTCTGGCAC AGAAATTAGG TCAGGGGATA 
CTGAATAATG CCTTCCGGCT GAGTCCTGCT CCTTCTAAAA CTCTTATGGA TAACTACGAA 
GTCTCCGGGG GGACAATAAA AGAGCTGGTG GAGGCCCTGA GACAGATGGG CTATACCGAA 
GCCATCGAAG TGATCCAGGC GGCCTTCTGC GCTCCGGAAA CTGCGGCCCC CAGCCCAGGG 
AAGGGTGCAC CTCAGACCCT CTCGCTGCCC CTCTCATCTG CCTCCACGAG GTCGCCAGTA 
GACGAGGTCC GAGACGACAG CATCTGTGAC AGCGGCGTGG AGACCTCCTT CCGCAGGCTC 
AGCTTCACAG AGTCTCTGAC CAGTGGCAGC TCCTTGCTGA CTCTTAACAA AGCGCCCCAC 
GAGTACGGGC AGGAAGGACC GATAGAGGGC AAAATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of MEK/ERK pathways through NF-?B activation is involved in interleukin-1?-induced cyclooxygenease-2 expression in canine dermal fibroblasts.
Veterinary immunology and immunopathology168(3-4)223-32(2015 Dec)
Tsuchiya H,Nakano R,Konno T,Okabayashi K,Narita T,Sugiya H

Short communication: molecular characterization of dog and cat p65 subunits of NF-kappaB.
Research in veterinary science99145-8(2015 Apr)
Ishikawa S,Takemitsu H,Li G,Mori N,Yamamoto I,Arai T

Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.
The Journal of veterinary medical science77(2)139-45(2015 Feb)
Pei Z,Wang J

Activation of nuclear factor-kappa B and cell adhesion molecule mRNA expression in duodenal mucosa of dogs with lymphocytic-plasmacytic enteritis.
Veterinary immunology and immunopathology154(3-4)145-52(2013 Aug)
Okanishi H,Kabeya H,Maruyama S,Kagawa Y,Watari T

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NFKB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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