AFDN cDNA ORF clone, Canis lupus familiaris(dog)

The following AFDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285927 XM_022419086.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe34496 XM_014113200.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe01031 XM_022419058.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe01032 XM_005615575.2
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe01032 XM_022419073.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285916 XM_022419033.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285917 XM_022419034.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285918 XM_022419039.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285919 XM_022419041.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285920 XM_022419044.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285921 XM_022419047.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285922 XM_022419055.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285923 XM_022419066.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285924 XM_022419071.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285925 XM_022419076.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe285926 XM_022419083.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34487 XM_014113172.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34488 XM_014113176.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34489 XM_014113178.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34490 XM_014113184.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34491 XM_014113187.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34492 XM_014113190.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34492 XM_022419031.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34493 XM_014113194.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34494 XM_014113196.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34495 XM_014113198.1
Latest version!
Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe34497 XM_014113201.1
Latest version!
Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285927
    Clone ID Related Accession (Same CDS sequence) XM_022419086.1
    Accession Version XM_022419086.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGTTACA GGATGAGGAG CGGAGGCGCC AGCAGCAGCT GGAAGAGATG
    CGCAAGCGAG AAGCAGAAGA TCGCGCCAGG CAGGAGGACG AGCGGCGCCG CCAGGAGGAG
    GAGAGAGCAA AGCGAGATGC TGAAGAAAAG AGGCGTCAGG AAGAAGGCTA TTACAGCCGC
    CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGTTGCT GGAGCCGGAG
    GAGCCGGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274794.1
    CDS22..5127
    Translation

    Target ORF information:

    RefSeq Version XM_022419086.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419086.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGTTACA GGATGAGGAG CGGAGGCGCC AGCAGCAGCT GGAAGAGATG
    CGCAAGCGAG AAGCAGAAGA TCGCGCCAGG CAGGAGGACG AGCGGCGCCG CCAGGAGGAG
    GAGAGAGCAA AGCGAGATGC TGAAGAAAAG AGGCGTCAGG AAGAAGGCTA TTACAGCCGC
    CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGTTGCT GGAGCCGGAG
    GAGCCGGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34496
    Clone ID Related Accession (Same CDS sequence) XM_014113200.1
    Accession Version XM_014113200.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GAAACAGAAG
    AATATTGCAG GGGCCCTTGC CTTCTGGATG GCCAATGCAT CTGAGCTGCT CAACTTCATT
    AAGCAGGACC GAGATCTTAG TCGAATCACA CTGGACGCCC AGGACGTTTT AGCACACTTG
    GTTCAGATGG CGTTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG
    CCAGCCTTCC TGGATGACCC CGAGGAGAAC AGCCTACAGA GACCAAAAAT AGATGATGTT
    TTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCTGCCCTG
    ACCATCCAGC TCTTCTCTCA GCTCTTCCAC TTTATCAACA TGTGGCTCTT CAACAGATTG
    GTGACCGACC CTGAGTCGGG ACTGTGCTCC CACTACTGGG GGGCGATTAT TCGCCAGCAG
    CTAGGACACG TGGAAGCTTG GGCAGAGAAG CAGGGGCTGG AGCTGGCAGC GGACTGTCAC
    CTCAGCAGGA TCGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC
    ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAATTCAC TGCAACTTCA AGCCTTGTTA
    CAGAATTACC ACTGTGCACC TGATGAGCCT TTTATTCCCA CGGACCTGAT AGAGAATGTG
    GTGGCTGTGG CTGAAAACAC GGCTGACGAG CTGGCGCGCA GTGATGGCCG GGAGGTGCAG
    CTGGAAGAGG ACCCCGATCT GCAGTTGCCT TTTCTTTTGC CAGAAGACGG CTATTCTTGT
    GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TAGACCCTTT GTGCCAGAGA
    GGATTTTGCA GGTTAATCCC TCATGCACGT TCACCAGGTA CTTGGACAAT ATATTTTGAG
    GGTGCAGATT ACGAGAGCCA TCTTCTGCGT GAGAACACGG AGCTGGCTCA GCCTCTGAGG
    AAAGAACCTG AAATAATCAC TGTGACTCTT AAGAAGCAAA ATGGGATGGG CCTTAGCATC
    GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTTGGAATCT ATGTCAAGTC TGTTGTAAAA
    GGCGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCGGGTG ACCAGCTCCT CAGTGTGGAC
    GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AGCTCATGAC AAGAACAAGT
    TCTGTGGTGA CACTAGAAGT AGCAAAGCAA GGAGCAATTT ATCACGGCCT CGCCACGCTG
    CTTAATCAGC CATCCCCAAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC
    CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCAAAACGG GTCACCTGAG
    AGCCCTCAGC TGCCTTGGGC AGAATACACT GAACCCAAGA AATTGCCTGG CGATGACAGA
    CTGATGAAGA ACCGGGCTGA TCACCGCTCC AGCCCCAACG TGGCAAATCA GCCTCCTAGC
    CCTGGAGGGA AAAGCGCATA CGCCCCTGGA ACAACAGCTA AGATAACGTC TGTCTCCACT
    GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCTTA CCCCATCCCC
    ACTCAGACAT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG
    GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CATGGACAAT
    AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AGGAGGAGCT TCGGGAGGAT
    AAGGCTTACC AACTAGAGCG GCATCGGATC GAGGCAGTCC TGGATCGCAA GTCTGATAGC
    GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCGAGCA CTTCCAGCCA AGAGCACCTG
    GACCACCCCT CCAAGGCCGG CACCCCCGCT TCCACTCTGA CCAAGAGTGG CCCTGGGCGC
    TGGAAGACGC CAGCCGCTGT GCAGCCCCTG CCTGTGGCTG GCTCCCAGCC GGTCCAAACT
    GACCTGCCCC CACCACCCCC GCCGCCCCCC ACGCACTACG CCGGAGACTT CGACGGGATC
    CCAACAGACC TGCCTCTCCC ACCACCTCCT GCTAGCCAGA CAGGACCTGC CTCTGCTCAG
    GTGGTGGCTG CAGAGAGGAA GAAGAGGGAG GAGCACCAGC GCTGGTATGA GAAGGAGAAG
    GCCCGTCTAG AGGAGGAGCG GGACAGGAAG CGCCGTGAGC AGGAGCGAAA GCTGGGCCAG
    ATGCGCTCCC AGTCACTGAA CCCAGCCCCG TTTTCTCCTG TAACTGTCCC CCATGTGAAG
    CCAGAAAAAC CTTCTACACT CCAGAGGCCC CAAGAGACGG TCATTCGGGA GCTGCAGCCC
    CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACAGT CAGCAAAGAG
    GAGCTGTCCT CAGGAGATAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CCGGGAGAAG
    CTAGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCAGGAGCTC
    CAGAATAAGC CCGAGCGCAG TGCAGAGGAA AACGATCGCC TGCGGAAACT CATGCTGGAG
    TGGCAGTTCC AGAAGAGGCT CCAGGAGTCC AAGCAGAAGG ACGAGGATGA CGAGGAGGAG
    GAGGATGATG ACGTGGATAC CATGCTCATC ATGCAGCGCC TCGAGGCCGA GCGAAGAGCA
    AGGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGGTTATGGT TCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968675.1
    CDS22..4848
    Translation

    Target ORF information:

    RefSeq Version XM_014113200.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GAAACAGAAG
    AATATTGCAG GGGCCCTTGC CTTCTGGATG GCCAATGCAT CTGAGCTGCT CAACTTCATT
    AAGCAGGACC GAGATCTTAG TCGAATCACA CTGGACGCCC AGGACGTTTT AGCACACTTG
    GTTCAGATGG CGTTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG
    CCAGCCTTCC TGGATGACCC CGAGGAGAAC AGCCTACAGA GACCAAAAAT AGATGATGTT
    TTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCTGCCCTG
    ACCATCCAGC TCTTCTCTCA GCTCTTCCAC TTTATCAACA TGTGGCTCTT CAACAGATTG
    GTGACCGACC CTGAGTCGGG ACTGTGCTCC CACTACTGGG GGGCGATTAT TCGCCAGCAG
    CTAGGACACG TGGAAGCTTG GGCAGAGAAG CAGGGGCTGG AGCTGGCAGC GGACTGTCAC
    CTCAGCAGGA TCGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC
    ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAATTCAC TGCAACTTCA AGCCTTGTTA
    CAGAATTACC ACTGTGCACC TGATGAGCCT TTTATTCCCA CGGACCTGAT AGAGAATGTG
    GTGGCTGTGG CTGAAAACAC GGCTGACGAG CTGGCGCGCA GTGATGGCCG GGAGGTGCAG
    CTGGAAGAGG ACCCCGATCT GCAGTTGCCT TTTCTTTTGC CAGAAGACGG CTATTCTTGT
    GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TAGACCCTTT GTGCCAGAGA
    GGATTTTGCA GGTTAATCCC TCATGCACGT TCACCAGGTA CTTGGACAAT ATATTTTGAG
    GGTGCAGATT ACGAGAGCCA TCTTCTGCGT GAGAACACGG AGCTGGCTCA GCCTCTGAGG
    AAAGAACCTG AAATAATCAC TGTGACTCTT AAGAAGCAAA ATGGGATGGG CCTTAGCATC
    GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTTGGAATCT ATGTCAAGTC TGTTGTAAAA
    GGCGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCGGGTG ACCAGCTCCT CAGTGTGGAC
    GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AGCTCATGAC AAGAACAAGT
    TCTGTGGTGA CACTAGAAGT AGCAAAGCAA GGAGCAATTT ATCACGGCCT CGCCACGCTG
    CTTAATCAGC CATCCCCAAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC
    CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCAAAACGG GTCACCTGAG
    AGCCCTCAGC TGCCTTGGGC AGAATACACT GAACCCAAGA AATTGCCTGG CGATGACAGA
    CTGATGAAGA ACCGGGCTGA TCACCGCTCC AGCCCCAACG TGGCAAATCA GCCTCCTAGC
    CCTGGAGGGA AAAGCGCATA CGCCCCTGGA ACAACAGCTA AGATAACGTC TGTCTCCACT
    GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCTTA CCCCATCCCC
    ACTCAGACAT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG
    GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CATGGACAAT
    AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AGGAGGAGCT TCGGGAGGAT
    AAGGCTTACC AACTAGAGCG GCATCGGATC GAGGCAGTCC TGGATCGCAA GTCTGATAGC
    GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCGAGCA CTTCCAGCCA AGAGCACCTG
    GACCACCCCT CCAAGGCCGG CACCCCCGCT TCCACTCTGA CCAAGAGTGG CCCTGGGCGC
    TGGAAGACGC CAGCCGCTGT GCAGCCCCTG CCTGTGGCTG GCTCCCAGCC GGTCCAAACT
    GACCTGCCCC CACCACCCCC GCCGCCCCCC ACGCACTACG CCGGAGACTT CGACGGGATC
    CCAACAGACC TGCCTCTCCC ACCACCTCCT GCTAGCCAGA CAGGACCTGC CTCTGCTCAG
    GTGGTGGCTG CAGAGAGGAA GAAGAGGGAG GAGCACCAGC GCTGGTATGA GAAGGAGAAG
    GCCCGTCTAG AGGAGGAGCG GGACAGGAAG CGCCGTGAGC AGGAGCGAAA GCTGGGCCAG
    ATGCGCTCCC AGTCACTGAA CCCAGCCCCG TTTTCTCCTG TAACTGTCCC CCATGTGAAG
    CCAGAAAAAC CTTCTACACT CCAGAGGCCC CAAGAGACGG TCATTCGGGA GCTGCAGCCC
    CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACAGT CAGCAAAGAG
    GAGCTGTCCT CAGGAGATAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CCGGGAGAAG
    CTAGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCAGGAGCTC
    CAGAATAAGC CCGAGCGCAG TGCAGAGGAA AACGATCGCC TGCGGAAACT CATGCTGGAG
    TGGCAGTTCC AGAAGAGGCT CCAGGAGTCC AAGCAGAAGG ACGAGGATGA CGAGGAGGAG
    GAGGATGATG ACGTGGATAC CATGCTCATC ATGCAGCGCC TCGAGGCCGA GCGAAGAGCA
    AGGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGGTTATGGT TCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01031
    Clone ID Related Accession (Same CDS sequence) XM_022419058.1
    Accession Version XM_022419058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGTTA CAGGATGAGG AGCGGAGGCG CCAGCAGCAG
    CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA GATCGCGCCA GGCAGGAGGA CGAGCGGCGC
    CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC
    TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGTTG
    CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG
    TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT CCCCCGCCTC AGCGGACCGC CTCCTCCCTC
    CAGGCCCAGG CCCTCTCCCC AGACTCCCTG TACACCGCCA GGCTCGTTGC GTACCACGAG
    GAGGAGGAGG AGGAGCACTG CAGTCTGGCA GGACCAAACT CTTACCTGGG ATCTGCCGGG
    ACAGCTGTTG GCACCCACGA TGCCTATCGG GACCCGAGAG AAAGACTGTC TAAAAGCCAC
    GAGGCAGATT TACCTGGGAG CTCTGGAGCC CCTGAAAACT TGACATTCAA AGAGCGCCAG
    CGTCTGTTCT CACAAGGTCA GGACGTGTCC AATAAAGTAA AAGCCTCTCG TAAATTAACA
    GAACTGGAAA ACGAACTGAA CACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274766.1
    CDS42..5411
    Translation

    Target ORF information:

    RefSeq Version XM_022419058.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGTTA CAGGATGAGG AGCGGAGGCG CCAGCAGCAG
    CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA GATCGCGCCA GGCAGGAGGA CGAGCGGCGC
    CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC
    TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGTTG
    CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG
    TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT CCCCCGCCTC AGCGGACCGC CTCCTCCCTC
    CAGGCCCAGG CCCTCTCCCC AGACTCCCTG TACACCGCCA GGCTCGTTGC GTACCACGAG
    GAGGAGGAGG AGGAGCACTG CAGTCTGGCA GGACCAAACT CTTACCTGGG ATCTGCCGGG
    ACAGCTGTTG GCACCCACGA TGCCTATCGG GACCCGAGAG AAAGACTGTC TAAAAGCCAC
    GAGGCAGATT TACCTGGGAG CTCTGGAGCC CCTGAAAACT TGACATTCAA AGAGCGCCAG
    CGTCTGTTCT CACAAGGTCA GGACGTGTCC AATAAAGTAA AAGCCTCTCG TAAATTAACA
    GAACTGGAAA ACGAACTGAA CACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01032
    Clone ID Related Accession (Same CDS sequence) XM_005615575.2 , XM_022419073.1
    Accession Version XM_005615575.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG
    AAGAATATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAATG CATCTGAGCT GCTCAACTTC
    ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACACTGGACG CCCAGGACGT TTTAGCACAC
    TTGGTTCAGA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC
    ATGCCAGCCT TCCTGGATGA CCCCGAGGAG AACAGCCTAC AGAGACCAAA AATAGATGAT
    GTTTTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTTATCA ACATGTGGCT CTTCAACAGA
    TTGGTGACCG ACCCTGAGTC GGGACTGTGC TCCCACTACT GGGGGGCGAT TATTCGCCAG
    CAGCTAGGAC ACGTGGAAGC TTGGGCAGAG AAGCAGGGGC TGGAGCTGGC AGCGGACTGT
    CACCTCAGCA GGATCGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT
    GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAATT CACTGCAACT TCAAGCCTTG
    TTACAGAATT ACCACTGTGC ACCTGATGAG CCTTTTATTC CCACGGACCT GATAGAGAAT
    GTGGTGGCTG TGGCTGAAAA CACGGCTGAC GAGCTGGCGC GCAGTGATGG CCGGGAGGTG
    CAGCTGGAAG AGGACCCCGA TCTGCAGTTG CCTTTTCTTT TGCCAGAAGA CGGCTATTCT
    TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TTTGTGCCAG
    AGAGGATTTT GCAGGTTAAT CCCTCATGCA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAGGGTGCAG ATTACGAGAG CCATCTTCTG CGTGAGAACA CGGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAGAAGC AAAATGGGAT GGGCCTTAGC
    ATCGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTTGTA
    AAAGGCGGTG CTGCGGATGT GGATGGACGC CTGGCTGCGG GTGACCAGCT CCTCAGTGTG
    GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAGCTCAT GACAAGAACA
    AGTTCTGTGG TGACACTAGA AGTAGCAAAG CAAGGAGCAA TTTATCACGG CCTCGCCACG
    CTGCTTAATC AGCCATCCCC AATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCAAAA CGGGTCACCT
    GAGAGCCCTC AGCTGCCTTG GGCAGAATAC ACTGAACCCA AGAAATTGCC TGGCGATGAC
    AGACTGATGA AGAACCGGGC TGATCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAAAGCGC ATACGCCCCT GGAACAACAG CTAAGATAAC GTCTGTCTCC
    ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC TTACCCCATC
    CCCACTCAGA CATACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACATGGAC
    AATAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAGGAGGA GCTTCGGGAG
    GATAAGGCTT ACCAACTAGA GCGGCATCGG ATCGAGGCAG TCCTGGATCG CAAGTCTGAT
    AGCGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCGA GCACTTCCAG CCAAGAGCAC
    CTGGACCACC CCTCCAAGGC CGGCACCCCC GCTTCCACTC TGACCAAGAG TGGCCCTGGG
    CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC CTGCCTGTGG CTGGCTCCCA GCCGGTCCAA
    ACTGACCTGC CCCCACCACC CCCGCCGCCC CCCACGCACT ACGCCGGAGA CTTCGACGGG
    ATCCCAACAG ACCTGCCTCT CCCACCACCT CCTGCTAGCC AGACAGGACC TGCCTCTGCT
    CAGGTGGTGG CTGCAGAGAG GAAGAAGAGG GAGGAGCACC AGCGCTGGTA TGAGAAGGAG
    AAGGCCCGTC TAGAGGAGGA GCGGGACAGG AAGCGCCGTG AGCAGGAGCG AAAGCTGGGC
    CAGATGCGCT CCCAGTCACT GAACCCAGCC CCGTTTTCTC CTGTAACTGT CCCCCATGTG
    AAGCCAGAAA AACCTTCTAC ACTCCAGAGG CCCCAAGAGA CGGTCATTCG GGAGCTGCAG
    CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC AGTCAGCAAA
    GAGGAGCTGT CCTCAGGAGA TAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCCGGGAG
    AAGCTAGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCAGGAG
    CTCCAGAATA AGCCCGAGCG CAGTGCAGAG GAAAACGATC GCCTGCGGAA ACTCATGCTG
    GAGTGGCAGT TCCAGAAGAG GCTCCAGGAG TCCAAGCAGA AGGACGAGGA TGACGAGGAG
    GAGGAGGATG ATGACGTGGA TACCATGCTC ATCATGCAGC GCCTCGAGGC CGAGCGAAGA
    GCAAGGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615632.1
    CDS28..5370
    Translation

    Target ORF information:

    RefSeq Version XM_005615575.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615575.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG
    AAGAATATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAATG CATCTGAGCT GCTCAACTTC
    ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACACTGGACG CCCAGGACGT TTTAGCACAC
    TTGGTTCAGA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC
    ATGCCAGCCT TCCTGGATGA CCCCGAGGAG AACAGCCTAC AGAGACCAAA AATAGATGAT
    GTTTTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTTATCA ACATGTGGCT CTTCAACAGA
    TTGGTGACCG ACCCTGAGTC GGGACTGTGC TCCCACTACT GGGGGGCGAT TATTCGCCAG
    CAGCTAGGAC ACGTGGAAGC TTGGGCAGAG AAGCAGGGGC TGGAGCTGGC AGCGGACTGT
    CACCTCAGCA GGATCGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT
    GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAATT CACTGCAACT TCAAGCCTTG
    TTACAGAATT ACCACTGTGC ACCTGATGAG CCTTTTATTC CCACGGACCT GATAGAGAAT
    GTGGTGGCTG TGGCTGAAAA CACGGCTGAC GAGCTGGCGC GCAGTGATGG CCGGGAGGTG
    CAGCTGGAAG AGGACCCCGA TCTGCAGTTG CCTTTTCTTT TGCCAGAAGA CGGCTATTCT
    TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TTTGTGCCAG
    AGAGGATTTT GCAGGTTAAT CCCTCATGCA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAGGGTGCAG ATTACGAGAG CCATCTTCTG CGTGAGAACA CGGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAGAAGC AAAATGGGAT GGGCCTTAGC
    ATCGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTTGTA
    AAAGGCGGTG CTGCGGATGT GGATGGACGC CTGGCTGCGG GTGACCAGCT CCTCAGTGTG
    GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAGCTCAT GACAAGAACA
    AGTTCTGTGG TGACACTAGA AGTAGCAAAG CAAGGAGCAA TTTATCACGG CCTCGCCACG
    CTGCTTAATC AGCCATCCCC AATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCAAAA CGGGTCACCT
    GAGAGCCCTC AGCTGCCTTG GGCAGAATAC ACTGAACCCA AGAAATTGCC TGGCGATGAC
    AGACTGATGA AGAACCGGGC TGATCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAAAGCGC ATACGCCCCT GGAACAACAG CTAAGATAAC GTCTGTCTCC
    ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC TTACCCCATC
    CCCACTCAGA CATACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACATGGAC
    AATAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAGGAGGA GCTTCGGGAG
    GATAAGGCTT ACCAACTAGA GCGGCATCGG ATCGAGGCAG TCCTGGATCG CAAGTCTGAT
    AGCGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCGA GCACTTCCAG CCAAGAGCAC
    CTGGACCACC CCTCCAAGGC CGGCACCCCC GCTTCCACTC TGACCAAGAG TGGCCCTGGG
    CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC CTGCCTGTGG CTGGCTCCCA GCCGGTCCAA
    ACTGACCTGC CCCCACCACC CCCGCCGCCC CCCACGCACT ACGCCGGAGA CTTCGACGGG
    ATCCCAACAG ACCTGCCTCT CCCACCACCT CCTGCTAGCC AGACAGGACC TGCCTCTGCT
    CAGGTGGTGG CTGCAGAGAG GAAGAAGAGG GAGGAGCACC AGCGCTGGTA TGAGAAGGAG
    AAGGCCCGTC TAGAGGAGGA GCGGGACAGG AAGCGCCGTG AGCAGGAGCG AAAGCTGGGC
    CAGATGCGCT CCCAGTCACT GAACCCAGCC CCGTTTTCTC CTGTAACTGT CCCCCATGTG
    AAGCCAGAAA AACCTTCTAC ACTCCAGAGG CCCCAAGAGA CGGTCATTCG GGAGCTGCAG
    CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC AGTCAGCAAA
    GAGGAGCTGT CCTCAGGAGA TAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCCGGGAG
    AAGCTAGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCAGGAG
    CTCCAGAATA AGCCCGAGCG CAGTGCAGAG GAAAACGATC GCCTGCGGAA ACTCATGCTG
    GAGTGGCAGT TCCAGAAGAG GCTCCAGGAG TCCAAGCAGA AGGACGAGGA TGACGAGGAG
    GAGGAGGATG ATGACGTGGA TACCATGCTC ATCATGCAGC GCCTCGAGGC CGAGCGAAGA
    GCAAGGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01032
    Clone ID Related Accession (Same CDS sequence) XM_005615575.2 , XM_022419073.1
    Accession Version XM_022419073.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG
    AAGAATATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAATG CATCTGAGCT GCTCAACTTC
    ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACACTGGACG CCCAGGACGT TTTAGCACAC
    TTGGTTCAGA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC
    ATGCCAGCCT TCCTGGATGA CCCCGAGGAG AACAGCCTAC AGAGACCAAA AATAGATGAT
    GTTTTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTTATCA ACATGTGGCT CTTCAACAGA
    TTGGTGACCG ACCCTGAGTC GGGACTGTGC TCCCACTACT GGGGGGCGAT TATTCGCCAG
    CAGCTAGGAC ACGTGGAAGC TTGGGCAGAG AAGCAGGGGC TGGAGCTGGC AGCGGACTGT
    CACCTCAGCA GGATCGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT
    GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAATT CACTGCAACT TCAAGCCTTG
    TTACAGAATT ACCACTGTGC ACCTGATGAG CCTTTTATTC CCACGGACCT GATAGAGAAT
    GTGGTGGCTG TGGCTGAAAA CACGGCTGAC GAGCTGGCGC GCAGTGATGG CCGGGAGGTG
    CAGCTGGAAG AGGACCCCGA TCTGCAGTTG CCTTTTCTTT TGCCAGAAGA CGGCTATTCT
    TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TTTGTGCCAG
    AGAGGATTTT GCAGGTTAAT CCCTCATGCA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAGGGTGCAG ATTACGAGAG CCATCTTCTG CGTGAGAACA CGGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAGAAGC AAAATGGGAT GGGCCTTAGC
    ATCGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTTGTA
    AAAGGCGGTG CTGCGGATGT GGATGGACGC CTGGCTGCGG GTGACCAGCT CCTCAGTGTG
    GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAGCTCAT GACAAGAACA
    AGTTCTGTGG TGACACTAGA AGTAGCAAAG CAAGGAGCAA TTTATCACGG CCTCGCCACG
    CTGCTTAATC AGCCATCCCC AATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCAAAA CGGGTCACCT
    GAGAGCCCTC AGCTGCCTTG GGCAGAATAC ACTGAACCCA AGAAATTGCC TGGCGATGAC
    AGACTGATGA AGAACCGGGC TGATCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAAAGCGC ATACGCCCCT GGAACAACAG CTAAGATAAC GTCTGTCTCC
    ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC TTACCCCATC
    CCCACTCAGA CATACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACATGGAC
    AATAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAGGAGGA GCTTCGGGAG
    GATAAGGCTT ACCAACTAGA GCGGCATCGG ATCGAGGCAG TCCTGGATCG CAAGTCTGAT
    AGCGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCGA GCACTTCCAG CCAAGAGCAC
    CTGGACCACC CCTCCAAGGC CGGCACCCCC GCTTCCACTC TGACCAAGAG TGGCCCTGGG
    CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC CTGCCTGTGG CTGGCTCCCA GCCGGTCCAA
    ACTGACCTGC CCCCACCACC CCCGCCGCCC CCCACGCACT ACGCCGGAGA CTTCGACGGG
    ATCCCAACAG ACCTGCCTCT CCCACCACCT CCTGCTAGCC AGACAGGACC TGCCTCTGCT
    CAGGTGGTGG CTGCAGAGAG GAAGAAGAGG GAGGAGCACC AGCGCTGGTA TGAGAAGGAG
    AAGGCCCGTC TAGAGGAGGA GCGGGACAGG AAGCGCCGTG AGCAGGAGCG AAAGCTGGGC
    CAGATGCGCT CCCAGTCACT GAACCCAGCC CCGTTTTCTC CTGTAACTGT CCCCCATGTG
    AAGCCAGAAA AACCTTCTAC ACTCCAGAGG CCCCAAGAGA CGGTCATTCG GGAGCTGCAG
    CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC AGTCAGCAAA
    GAGGAGCTGT CCTCAGGAGA TAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCCGGGAG
    AAGCTAGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCAGGAG
    CTCCAGAATA AGCCCGAGCG CAGTGCAGAG GAAAACGATC GCCTGCGGAA ACTCATGCTG
    GAGTGGCAGT TCCAGAAGAG GCTCCAGGAG TCCAAGCAGA AGGACGAGGA TGACGAGGAG
    GAGGAGGATG ATGACGTGGA TACCATGCTC ATCATGCAGC GCCTCGAGGC CGAGCGAAGA
    GCAAGGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274781.1
    CDS42..5384
    Translation

    Target ORF information:

    RefSeq Version XM_022419073.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419073.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG
    AAGAATATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAATG CATCTGAGCT GCTCAACTTC
    ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACACTGGACG CCCAGGACGT TTTAGCACAC
    TTGGTTCAGA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC
    ATGCCAGCCT TCCTGGATGA CCCCGAGGAG AACAGCCTAC AGAGACCAAA AATAGATGAT
    GTTTTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTTATCA ACATGTGGCT CTTCAACAGA
    TTGGTGACCG ACCCTGAGTC GGGACTGTGC TCCCACTACT GGGGGGCGAT TATTCGCCAG
    CAGCTAGGAC ACGTGGAAGC TTGGGCAGAG AAGCAGGGGC TGGAGCTGGC AGCGGACTGT
    CACCTCAGCA GGATCGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT
    GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAATT CACTGCAACT TCAAGCCTTG
    TTACAGAATT ACCACTGTGC ACCTGATGAG CCTTTTATTC CCACGGACCT GATAGAGAAT
    GTGGTGGCTG TGGCTGAAAA CACGGCTGAC GAGCTGGCGC GCAGTGATGG CCGGGAGGTG
    CAGCTGGAAG AGGACCCCGA TCTGCAGTTG CCTTTTCTTT TGCCAGAAGA CGGCTATTCT
    TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TTTGTGCCAG
    AGAGGATTTT GCAGGTTAAT CCCTCATGCA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAGGGTGCAG ATTACGAGAG CCATCTTCTG CGTGAGAACA CGGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAGAAGC AAAATGGGAT GGGCCTTAGC
    ATCGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTTGTA
    AAAGGCGGTG CTGCGGATGT GGATGGACGC CTGGCTGCGG GTGACCAGCT CCTCAGTGTG
    GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAGCTCAT GACAAGAACA
    AGTTCTGTGG TGACACTAGA AGTAGCAAAG CAAGGAGCAA TTTATCACGG CCTCGCCACG
    CTGCTTAATC AGCCATCCCC AATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCAAAA CGGGTCACCT
    GAGAGCCCTC AGCTGCCTTG GGCAGAATAC ACTGAACCCA AGAAATTGCC TGGCGATGAC
    AGACTGATGA AGAACCGGGC TGATCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAAAGCGC ATACGCCCCT GGAACAACAG CTAAGATAAC GTCTGTCTCC
    ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC TTACCCCATC
    CCCACTCAGA CATACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACATGGAC
    AATAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAGGAGGA GCTTCGGGAG
    GATAAGGCTT ACCAACTAGA GCGGCATCGG ATCGAGGCAG TCCTGGATCG CAAGTCTGAT
    AGCGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCGA GCACTTCCAG CCAAGAGCAC
    CTGGACCACC CCTCCAAGGC CGGCACCCCC GCTTCCACTC TGACCAAGAG TGGCCCTGGG
    CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC CTGCCTGTGG CTGGCTCCCA GCCGGTCCAA
    ACTGACCTGC CCCCACCACC CCCGCCGCCC CCCACGCACT ACGCCGGAGA CTTCGACGGG
    ATCCCAACAG ACCTGCCTCT CCCACCACCT CCTGCTAGCC AGACAGGACC TGCCTCTGCT
    CAGGTGGTGG CTGCAGAGAG GAAGAAGAGG GAGGAGCACC AGCGCTGGTA TGAGAAGGAG
    AAGGCCCGTC TAGAGGAGGA GCGGGACAGG AAGCGCCGTG AGCAGGAGCG AAAGCTGGGC
    CAGATGCGCT CCCAGTCACT GAACCCAGCC CCGTTTTCTC CTGTAACTGT CCCCCATGTG
    AAGCCAGAAA AACCTTCTAC ACTCCAGAGG CCCCAAGAGA CGGTCATTCG GGAGCTGCAG
    CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC AGTCAGCAAA
    GAGGAGCTGT CCTCAGGAGA TAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCCGGGAG
    AAGCTAGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCAGGAG
    CTCCAGAATA AGCCCGAGCG CAGTGCAGAG GAAAACGATC GCCTGCGGAA ACTCATGCTG
    GAGTGGCAGT TCCAGAAGAG GCTCCAGGAG TCCAAGCAGA AGGACGAGGA TGACGAGGAG
    GAGGAGGATG ATGACGTGGA TACCATGCTC ATCATGCAGC GCCTCGAGGC CGAGCGAAGA
    GCAAGGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285916
    Clone ID Related Accession (Same CDS sequence) XM_022419033.1
    Accession Version XM_022419033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274741.1
    CDS43..5445
    Translation

    Target ORF information:

    RefSeq Version XM_022419033.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285917
    Clone ID Related Accession (Same CDS sequence) XM_022419034.1
    Accession Version XM_022419034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274742.1
    CDS43..5445
    Translation

    Target ORF information:

    RefSeq Version XM_022419034.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285918
    Clone ID Related Accession (Same CDS sequence) XM_022419039.1
    Accession Version XM_022419039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274747.1
    CDS43..5445
    Translation

    Target ORF information:

    RefSeq Version XM_022419039.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285919
    Clone ID Related Accession (Same CDS sequence) XM_022419041.1
    Accession Version XM_022419041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC TATTACAGCC GCCTGGAAGC CGAGAGGCGC
    AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA
    CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT
    CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCCCAGG CCCTCTCCCC AGACTCCCTG
    TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGCACTG CAGTCTGGCA
    GGACCAAACT CTTACCTGGG ATCTGCCGGG ACAGCTGTTG GCACCCACGA TGCCTATCGG
    GACCCGAGAG AAAGACTGTC TAAAAGCCAC GAGGCAGATT TACCTGGGAG CTCTGGAGCC
    CCTGAAAACT TGACATTCAA AGAGCGCCAG CGTCTGTTCT CACAAGGTCA GGACGTGTCC
    AATAAAGTAA AAGCCTCTCG TAAATTAACA GAACTGGAAA ACGAACTGAA CACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274749.1
    CDS43..5442
    Translation

    Target ORF information:

    RefSeq Version XM_022419041.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC TATTACAGCC GCCTGGAAGC CGAGAGGCGC
    AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA
    CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT
    CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCCCAGG CCCTCTCCCC AGACTCCCTG
    TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGCACTG CAGTCTGGCA
    GGACCAAACT CTTACCTGGG ATCTGCCGGG ACAGCTGTTG GCACCCACGA TGCCTATCGG
    GACCCGAGAG AAAGACTGTC TAAAAGCCAC GAGGCAGATT TACCTGGGAG CTCTGGAGCC
    CCTGAAAACT TGACATTCAA AGAGCGCCAG CGTCTGTTCT CACAAGGTCA GGACGTGTCC
    AATAAAGTAA AAGCCTCTCG TAAATTAACA GAACTGGAAA ACGAACTGAA CACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285920
    Clone ID Related Accession (Same CDS sequence) XM_022419044.1
    Accession Version XM_022419044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGGG AGAAGGAATC ACTTTGCCTA CTACAACTAT
    CACACTTATG AAGATGGCTC TGACTCCAGA GATAAGCCCA AGCTTTACCG CCTTCAGTTG
    AGCGTTACTG AGGTTGGGAC GGAAAAGTTT GATGAGAACT CCATCCAGCT GTTCGGATCA
    GGAATTCAGC CCCACCACTG CGACCTCACA AACATGGATG GAGTTGTCAC TGTAACGCCG
    AGAAGCATGG ATGCTGAGAC TTACGTGGAA GGACAGCGCA TCTCGGAGAC AACCATGCTA
    CAGAGCGGGA TGAAAGTGCA GTTTGGGGCG TCACACGTGT TCAAGTTTGT GGATCCCAGC
    CAGGACCACG CTCTGGCAAA GCGATCTGTG GATGGAAGCC TGACCGCCAA GGGTCCCAGA
    CATAAACCTG GAATTGTTCA AGAGACAACA TTTGATTTAG GAGGAGATGT TCACAGTGGA
    ACAGCATTAC CAACAAGTAA GAGCACCACT CGGCTGGACG GCGACAGGGT ATTGTCTGCC
    TCCAGCACAG CGGAGCGAGG GATGGTGAAG CCGATGATGA GAGTAGAGCA GCAGCAGGAC
    TACCGCAGGC AAGAAAACAG AACTCAGGAT GCTCCTGGGC CTGAGCTGAT ACTGCCTGCA
    AGCATTGAAT TCAGGGAAAG TTCTGAAGAT TCATTTTTAT CTGCCATTAT AAACTATACT
    AATAGCTCTA CAGTCCATTT TAAGTTGTCA CCTACATATG TATTATATAT GGCATGTCGG
    TATGTATTGT CAAGCCAGTA CAGACCCGAC GTCAGCCCTA CAGAGCGCAC TCACAAAGTG
    ATTGCAATAG TCAACAAGAT GGTGAGCATG ATGGAGGGGG TGATTCAGGA AGTAGACCAG
    GTTGATCAGA AACAGAAGAA TATTGCAGGG GCCCTTGCCT TCTGGATGGC CAATGCATCT
    GAGCTGCTCA ACTTCATTAA GCAGGACCGA GATCTTAGTC GAATCACACT GGACGCCCAG
    GACGTTTTAG CACACTTGGT TCAGATGGCG TTTAAATATC TGGTTCATTG TCTTCAATCA
    GAACTGAATA ATTACATGCC AGCCTTCCTG GATGACCCCG AGGAGAACAG CCTACAGAGA
    CCAAAAATAG ATGATGTTTT GCACACGCTC ACGGGAGCCA TGTCTTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT TATCAACATG
    TGGCTCTTCA ACAGATTGGT GACCGACCCT GAGTCGGGAC TGTGCTCCCA CTACTGGGGG
    GCGATTATTC GCCAGCAGCT AGGACACGTG GAAGCTTGGG CAGAGAAGCA GGGGCTGGAG
    CTGGCAGCGG ACTGTCACCT CAGCAGGATC GTGCAGGCAA CCACTTTGCT TACAATGGAT
    AAATACGCAC CTGATGACAT TCCAAATATA AACAGTACCT GCTTTAAGTT AAATTCACTG
    CAACTTCAAG CCTTGTTACA GAATTACCAC TGTGCACCTG ATGAGCCTTT TATTCCCACG
    GACCTGATAG AGAATGTGGT GGCTGTGGCT GAAAACACGG CTGACGAGCT GGCGCGCAGT
    GATGGCCGGG AGGTGCAGCT GGAAGAGGAC CCCGATCTGC AGTTGCCTTT TCTTTTGCCA
    GAAGACGGCT ATTCTTGTGA TGTTGTCAGG AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTTTGT GCCAGAGAGG ATTTTGCAGG TTAATCCCTC ATGCACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAGGG TGCAGATTAC GAGAGCCATC TTCTGCGTGA GAACACGGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACTCTTAA GAAGCAAAAT
    GGGATGGGCC TTAGCATCGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT TGGAATCTAT
    GTCAAGTCTG TTGTAAAAGG CGGTGCTGCG GATGTGGATG GACGCCTGGC TGCGGGTGAC
    CAGCTCCTCA GTGTGGACGG ACGAAGTCTG GTAGGACTCT CTCAAGAAAG GGCGGCAGAG
    CTCATGACAA GAACAAGTTC TGTGGTGACA CTAGAAGTAG CAAAGCAAGG AGCAATTTAT
    CACGGCCTCG CCACGCTGCT TAATCAGCCA TCCCCAATGA TGCAAAGAAT TTCAGACCGT
    CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGTTTCG AGCTCTATAA TAATTCAGCT
    CAAAACGGGT CACCTGAGAG CCCTCAGCTG CCTTGGGCAG AATACACTGA ACCCAAGAAA
    TTGCCTGGCG ATGACAGACT GATGAAGAAC CGGGCTGATC ACCGCTCCAG CCCCAACGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAA AGCGCATACG CCCCTGGAAC AACAGCTAAG
    ATAACGTCTG TCTCCACTGG GAACCTCTGC ACTGAGGAAC AGACCCCTCC ACCTAGACCT
    GAGGCTTACC CCATCCCCAC TCAGACATAC ACCAGAGAGT ATTTCACCTT CCCAGCTTCC
    AAATCCCAGG ACCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTACGA GGAGAAGCCA
    CACATGCACA TGGACAATAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC CCGTTCCCAG
    GAGGAGCTTC GGGAGGATAA GGCTTACCAA CTAGAGCGGC ATCGGATCGA GGCAGTCCTG
    GATCGCAAGT CTGATAGCGA TATGTGGATC CATCAGAGCT CCTCAGTGGG CTCGAGCACT
    TCCAGCCAAG AGCACCTGGA CCACCCCTCC AAGGCCGGCA CCCCCGCTTC CACTCTGACC
    AAGAGTGGCC CTGGGCGCTG GAAGACGCCA GCCGCTGTGC AGCCCCTGCC TGTGGCTGGC
    TCCCAGCCGG TCCAAACTGA CCTGCCCCCA CCACCCCCGC CGCCCCCCAC GCACTACGCC
    GGAGACTTCG ACGGGATCCC AACAGACCTG CCTCTCCCAC CACCTCCTGC TAGCCAGACA
    GGACCTGCCT CTGCTCAGGT GGTGGCTGCA GAGAGGAAGA AGAGGGAGGA GCACCAGCGC
    TGGTATGAGA AGGAGAAGGC CCGTCTAGAG GAGGAGCGGG ACAGGAAGCG CCGTGAGCAG
    GAGCGAAAGC TGGGCCAGAT GCGCTCCCAG TCACTGAACC CAGCCCCGTT TTCTCCTGTA
    ACTGTCCCCC ATGTGAAGCC AGAAAAACCT TCTACACTCC AGAGGCCCCA AGAGACGGTC
    ATTCGGGAGC TGCAGCCCCA GCAGCAGCCC CGCACGATTG AGCGCAGAGA CTTACAGTAC
    ATTACAGTCA GCAAAGAGGA GCTGTCCTCA GGAGATAGTC TGTCCCCAGA CCCGTGGAAG
    CGGGATGCCC GGGAGAAGCT AGAGAAGCAG CAGCAGATGC ACATCGTGGA TATGCTGAGC
    AGGGAGATCC AGGAGCTCCA GAATAAGCCC GAGCGCAGTG CAGAGGAAAA CGATCGCCTG
    CGGAAACTCA TGCTGGAGTG GCAGTTCCAG AAGAGGCTCC AGGAGTCCAA GCAGAAGGAC
    GAGGATGACG AGGAGGAGGA GGATGATGAC GTGGATACCA TGCTCATCAT GCAGCGCCTC
    GAGGCCGAGC GAAGAGCAAG GCAGACTGCT CTGCCAGCAA TCTCTGTTCT AGATTTGTTA
    CAGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC TATTACAGCC GCCTGGAAGC CGAGAGGCGC
    AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA
    CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT
    CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCCCAGG CCCTCTCCCC AGACTCCCTG
    TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGCACTG CAGTCTGGCA
    GGACCAAACT CTTACCTGGG ATCTGCCGGG ACAGCTGTTG GCACCCACGA TGCCTATCGG
    GACCCGAGAG AAAGACTGTC TAAAAGCCAC GAGGCAGATT TACCTGGGAG CTCTGGAGCC
    CCTGAAAACT TGACATTCAA AGAGCGCCAG CGTCTGTTCT CACAAGGTCA GGACGTGTCC
    AATAAAGTAA AAGCCTCTCG TAAATTAACA GAACTGGAAA ACGAACTGAA CACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274752.1
    CDS43..5442
    Translation

    Target ORF information:

    RefSeq Version XM_022419044.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGGG AGAAGGAATC ACTTTGCCTA CTACAACTAT
    CACACTTATG AAGATGGCTC TGACTCCAGA GATAAGCCCA AGCTTTACCG CCTTCAGTTG
    AGCGTTACTG AGGTTGGGAC GGAAAAGTTT GATGAGAACT CCATCCAGCT GTTCGGATCA
    GGAATTCAGC CCCACCACTG CGACCTCACA AACATGGATG GAGTTGTCAC TGTAACGCCG
    AGAAGCATGG ATGCTGAGAC TTACGTGGAA GGACAGCGCA TCTCGGAGAC AACCATGCTA
    CAGAGCGGGA TGAAAGTGCA GTTTGGGGCG TCACACGTGT TCAAGTTTGT GGATCCCAGC
    CAGGACCACG CTCTGGCAAA GCGATCTGTG GATGGAAGCC TGACCGCCAA GGGTCCCAGA
    CATAAACCTG GAATTGTTCA AGAGACAACA TTTGATTTAG GAGGAGATGT TCACAGTGGA
    ACAGCATTAC CAACAAGTAA GAGCACCACT CGGCTGGACG GCGACAGGGT ATTGTCTGCC
    TCCAGCACAG CGGAGCGAGG GATGGTGAAG CCGATGATGA GAGTAGAGCA GCAGCAGGAC
    TACCGCAGGC AAGAAAACAG AACTCAGGAT GCTCCTGGGC CTGAGCTGAT ACTGCCTGCA
    AGCATTGAAT TCAGGGAAAG TTCTGAAGAT TCATTTTTAT CTGCCATTAT AAACTATACT
    AATAGCTCTA CAGTCCATTT TAAGTTGTCA CCTACATATG TATTATATAT GGCATGTCGG
    TATGTATTGT CAAGCCAGTA CAGACCCGAC GTCAGCCCTA CAGAGCGCAC TCACAAAGTG
    ATTGCAATAG TCAACAAGAT GGTGAGCATG ATGGAGGGGG TGATTCAGGA AGTAGACCAG
    GTTGATCAGA AACAGAAGAA TATTGCAGGG GCCCTTGCCT TCTGGATGGC CAATGCATCT
    GAGCTGCTCA ACTTCATTAA GCAGGACCGA GATCTTAGTC GAATCACACT GGACGCCCAG
    GACGTTTTAG CACACTTGGT TCAGATGGCG TTTAAATATC TGGTTCATTG TCTTCAATCA
    GAACTGAATA ATTACATGCC AGCCTTCCTG GATGACCCCG AGGAGAACAG CCTACAGAGA
    CCAAAAATAG ATGATGTTTT GCACACGCTC ACGGGAGCCA TGTCTTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT TATCAACATG
    TGGCTCTTCA ACAGATTGGT GACCGACCCT GAGTCGGGAC TGTGCTCCCA CTACTGGGGG
    GCGATTATTC GCCAGCAGCT AGGACACGTG GAAGCTTGGG CAGAGAAGCA GGGGCTGGAG
    CTGGCAGCGG ACTGTCACCT CAGCAGGATC GTGCAGGCAA CCACTTTGCT TACAATGGAT
    AAATACGCAC CTGATGACAT TCCAAATATA AACAGTACCT GCTTTAAGTT AAATTCACTG
    CAACTTCAAG CCTTGTTACA GAATTACCAC TGTGCACCTG ATGAGCCTTT TATTCCCACG
    GACCTGATAG AGAATGTGGT GGCTGTGGCT GAAAACACGG CTGACGAGCT GGCGCGCAGT
    GATGGCCGGG AGGTGCAGCT GGAAGAGGAC CCCGATCTGC AGTTGCCTTT TCTTTTGCCA
    GAAGACGGCT ATTCTTGTGA TGTTGTCAGG AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTTTGT GCCAGAGAGG ATTTTGCAGG TTAATCCCTC ATGCACGTTC ACCAGGTACT
    TGGACAATAT ATTTTGAGGG TGCAGATTAC GAGAGCCATC TTCTGCGTGA GAACACGGAG
    CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACTCTTAA GAAGCAAAAT
    GGGATGGGCC TTAGCATCGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT TGGAATCTAT
    GTCAAGTCTG TTGTAAAAGG CGGTGCTGCG GATGTGGATG GACGCCTGGC TGCGGGTGAC
    CAGCTCCTCA GTGTGGACGG ACGAAGTCTG GTAGGACTCT CTCAAGAAAG GGCGGCAGAG
    CTCATGACAA GAACAAGTTC TGTGGTGACA CTAGAAGTAG CAAAGCAAGG AGCAATTTAT
    CACGGCCTCG CCACGCTGCT TAATCAGCCA TCCCCAATGA TGCAAAGAAT TTCAGACCGT
    CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGTTTCG AGCTCTATAA TAATTCAGCT
    CAAAACGGGT CACCTGAGAG CCCTCAGCTG CCTTGGGCAG AATACACTGA ACCCAAGAAA
    TTGCCTGGCG ATGACAGACT GATGAAGAAC CGGGCTGATC ACCGCTCCAG CCCCAACGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAA AGCGCATACG CCCCTGGAAC AACAGCTAAG
    ATAACGTCTG TCTCCACTGG GAACCTCTGC ACTGAGGAAC AGACCCCTCC ACCTAGACCT
    GAGGCTTACC CCATCCCCAC TCAGACATAC ACCAGAGAGT ATTTCACCTT CCCAGCTTCC
    AAATCCCAGG ACCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTACGA GGAGAAGCCA
    CACATGCACA TGGACAATAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC CCGTTCCCAG
    GAGGAGCTTC GGGAGGATAA GGCTTACCAA CTAGAGCGGC ATCGGATCGA GGCAGTCCTG
    GATCGCAAGT CTGATAGCGA TATGTGGATC CATCAGAGCT CCTCAGTGGG CTCGAGCACT
    TCCAGCCAAG AGCACCTGGA CCACCCCTCC AAGGCCGGCA CCCCCGCTTC CACTCTGACC
    AAGAGTGGCC CTGGGCGCTG GAAGACGCCA GCCGCTGTGC AGCCCCTGCC TGTGGCTGGC
    TCCCAGCCGG TCCAAACTGA CCTGCCCCCA CCACCCCCGC CGCCCCCCAC GCACTACGCC
    GGAGACTTCG ACGGGATCCC AACAGACCTG CCTCTCCCAC CACCTCCTGC TAGCCAGACA
    GGACCTGCCT CTGCTCAGGT GGTGGCTGCA GAGAGGAAGA AGAGGGAGGA GCACCAGCGC
    TGGTATGAGA AGGAGAAGGC CCGTCTAGAG GAGGAGCGGG ACAGGAAGCG CCGTGAGCAG
    GAGCGAAAGC TGGGCCAGAT GCGCTCCCAG TCACTGAACC CAGCCCCGTT TTCTCCTGTA
    ACTGTCCCCC ATGTGAAGCC AGAAAAACCT TCTACACTCC AGAGGCCCCA AGAGACGGTC
    ATTCGGGAGC TGCAGCCCCA GCAGCAGCCC CGCACGATTG AGCGCAGAGA CTTACAGTAC
    ATTACAGTCA GCAAAGAGGA GCTGTCCTCA GGAGATAGTC TGTCCCCAGA CCCGTGGAAG
    CGGGATGCCC GGGAGAAGCT AGAGAAGCAG CAGCAGATGC ACATCGTGGA TATGCTGAGC
    AGGGAGATCC AGGAGCTCCA GAATAAGCCC GAGCGCAGTG CAGAGGAAAA CGATCGCCTG
    CGGAAACTCA TGCTGGAGTG GCAGTTCCAG AAGAGGCTCC AGGAGTCCAA GCAGAAGGAC
    GAGGATGACG AGGAGGAGGA GGATGATGAC GTGGATACCA TGCTCATCAT GCAGCGCCTC
    GAGGCCGAGC GAAGAGCAAG GCAGACTGCT CTGCCAGCAA TCTCTGTTCT AGATTTGTTA
    CAGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC TATTACAGCC GCCTGGAAGC CGAGAGGCGC
    AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA
    CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT
    CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCCCAGG CCCTCTCCCC AGACTCCCTG
    TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGCACTG CAGTCTGGCA
    GGACCAAACT CTTACCTGGG ATCTGCCGGG ACAGCTGTTG GCACCCACGA TGCCTATCGG
    GACCCGAGAG AAAGACTGTC TAAAAGCCAC GAGGCAGATT TACCTGGGAG CTCTGGAGCC
    CCTGAAAACT TGACATTCAA AGAGCGCCAG CGTCTGTTCT CACAAGGTCA GGACGTGTCC
    AATAAAGTAA AAGCCTCTCG TAAATTAACA GAACTGGAAA ACGAACTGAA CACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285921
    Clone ID Related Accession (Same CDS sequence) XM_022419047.1
    Accession Version XM_022419047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    GATGAGGAGC GGAGGCGCCA GCAGCAGCTG GAAGAGATGC GCAAGCGAGA AGCAGAAGAT
    CGCGCCAGGC AGGAGGACGA GCGGCGCCGC CAGGAGGAGG AGAGAGCAAA GCGAGATGCT
    GAAGAAAAGA GGCGTCAGGA AGAAGGCTAT TACAGCCGCC TGGAAGCCGA GAGGCGCAGA
    CAGCACGCGG AGGCCGAGCG CAGGTTGCTG GAGCCGGAGG AGCCGGGTCT GTGCAGACCT
    CCGCTGCCGC GGGGCTACAA GCTCCCGTCC CCGTCCCCTC CGGCTGCCGC TCCTCCTCCC
    CCGCCTCAGC GGACCGCCTC CTCCCTCCAG GCCCAGGCCC TCTCCCCAGA CTCCCTGTAC
    ACCGCCAGGC TCGTTGCGTA CCACGAGGAG GAGGAGGAGG AGCACTGCAG TCTGGCAGGA
    CCAAACTCTT ACCTGGGATC TGCCGGGACA GCTGTTGGCA CCCACGATGC CTATCGGGAC
    CCGAGAGAAA GACTGTCTAA AAGCCACGAG GCAGATTTAC CTGGGAGCTC TGGAGCCCCT
    GAAAACTTGA CATTCAAAGA GCGCCAGCGT CTGTTCTCAC AAGGTCAGGA CGTGTCCAAT
    AAAGTAAAAG CCTCTCGTAA ATTAACAGAA CTGGAAAACG AACTGAACAC CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274755.1
    CDS43..5439
    Translation

    Target ORF information:

    RefSeq Version XM_022419047.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    GATGAGGAGC GGAGGCGCCA GCAGCAGCTG GAAGAGATGC GCAAGCGAGA AGCAGAAGAT
    CGCGCCAGGC AGGAGGACGA GCGGCGCCGC CAGGAGGAGG AGAGAGCAAA GCGAGATGCT
    GAAGAAAAGA GGCGTCAGGA AGAAGGCTAT TACAGCCGCC TGGAAGCCGA GAGGCGCAGA
    CAGCACGCGG AGGCCGAGCG CAGGTTGCTG GAGCCGGAGG AGCCGGGTCT GTGCAGACCT
    CCGCTGCCGC GGGGCTACAA GCTCCCGTCC CCGTCCCCTC CGGCTGCCGC TCCTCCTCCC
    CCGCCTCAGC GGACCGCCTC CTCCCTCCAG GCCCAGGCCC TCTCCCCAGA CTCCCTGTAC
    ACCGCCAGGC TCGTTGCGTA CCACGAGGAG GAGGAGGAGG AGCACTGCAG TCTGGCAGGA
    CCAAACTCTT ACCTGGGATC TGCCGGGACA GCTGTTGGCA CCCACGATGC CTATCGGGAC
    CCGAGAGAAA GACTGTCTAA AAGCCACGAG GCAGATTTAC CTGGGAGCTC TGGAGCCCCT
    GAAAACTTGA CATTCAAAGA GCGCCAGCGT CTGTTCTCAC AAGGTCAGGA CGTGTCCAAT
    AAAGTAAAAG CCTCTCGTAA ATTAACAGAA CTGGAAAACG AACTGAACAC CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285922
    Clone ID Related Accession (Same CDS sequence) XM_022419055.1
    Accession Version XM_022419055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG
    AAGAATATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAATG CATCTGAGCT GCTCAACTTC
    ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACACTGGACG CCCAGGACGT TTTAGCACAC
    TTGGTTCAGA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC
    ATGCCAGCCT TCCTGGATGA CCCCGAGGAG AACAGCCTAC AGAGACCAAA AATAGATGAT
    GTTTTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTTATCA ACATGTGGCT CTTCAACAGA
    TTGGTGACCG ACCCTGAGTC GGGACTGTGC TCCCACTACT GGGGGGCGAT TATTCGCCAG
    CAGCTAGGAC ACGTGGAAGC TTGGGCAGAG AAGCAGGGGC TGGAGCTGGC AGCGGACTGT
    CACCTCAGCA GGATCGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT
    GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAATT CACTGCAACT TCAAGCCTTG
    TTACAGAATT ACCACTGTGC ACCTGATGAG CCTTTTATTC CCACGGACCT GATAGAGAAT
    GTGGTGGCTG TGGCTGAAAA CACGGCTGAC GAGCTGGCGC GCAGTGATGG CCGGGAGGTG
    CAGCTGGAAG AGGACCCCGA TCTGCAGTTG CCTTTTCTTT TGCCAGAAGA CGGCTATTCT
    TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TTTGTGCCAG
    AGAGGATTTT GCAGGTTAAT CCCTCATGCA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAGGGTGCAG ATTACGAGAG CCATCTTCTG CGTGAGAACA CGGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAGAAGC AAAATGGGAT GGGCCTTAGC
    ATCGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTTGTA
    AAAGGCGGTG CTGCGGATGT GGATGGACGC CTGGCTGCGG GTGACCAGCT CCTCAGTGTG
    GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAGCTCAT GACAAGAACA
    AGTTCTGTGG TGACACTAGA AGTAGCAAAG CAAGGAGCAA TTTATCACGG CCTCGCCACG
    CTGCTTAATC AGCCATCCCC AATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCAAAA CGGGTCACCT
    GAGAGCCCTC AGCTGCCTTG GGCAGAATAC ACTGAACCCA AGAAATTGCC TGGCGATGAC
    AGACTGATGA AGAACCGGGC TGATCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAAAGCGC ATACGCCCCT GGAACAACAG CTAAGATAAC GTCTGTCTCC
    ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC TTACCCCATC
    CCCACTCAGA CATACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACATGGAC
    AATAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAGGAGGA GCTTCGGGAG
    GATAAGGCTT ACCAACTAGA GCGGCATCGG ATCGAGGCAG TCCTGGATCG CAAGTCTGAT
    AGCGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCGA GCACTTCCAG CCAAGAGCAC
    CTGGACCACC CCTCCAAGGC CGGCACCCCC GCTTCCACTC TGACCAAGAG TGGCCCTGGG
    CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC CTGCCTGTGG CTGGCTCCCA GCCGGTCCAA
    ACTGACCTGC CCCCACCACC CCCGCCGCCC CCCACGCACT ACGCCGGAGA CTTCGACGGG
    ATCCCAACAG ACCTGCCTCT CCCACCACCT CCTGCTAGCC AGACAGGACC TGCCTCTGCT
    CAGGTGGTGG CTGCAGAGAG GAAGAAGAGG GAGGAGCACC AGCGCTGGTA TGAGAAGGAG
    AAGGCCCGTC TAGAGGAGGA GCGGGACAGG AAGCGCCGTG AGCAGGAGCG AAAGCTGGGC
    CAGATGCGCT CCCAGTCACT GAACCCAGCC CCGTTTTCTC CTGTAACTGT CCCCCATGTG
    AAGCCAGAAA AACCTTCTAC ACTCCAGAGG CCCCAAGAGA CGGTCATTCG GGAGCTGCAG
    CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC AGTCAGCAAA
    GAGGAGCTGT CCTCAGGAGA TAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCCGGGAG
    AAGCTAGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCAGGAG
    CTCCAGAATA AGCCCGAGCG CAGTGCAGAG GAAAACGATC GCCTGCGGAA ACTCATGCTG
    GAGTGGCAGT TCCAGAAGAG GCTCCAGGAG TCCAAGCAGA AGGACGAGGA TGACGAGGAG
    GAGGAGGATG ATGACGTGGA TACCATGCTC ATCATGCAGC GCCTCGAGGC CGAGCGAAGA
    GCAAGGCAGA CTGCTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA TGAGGAGCGG
    AGGCGCCAGC AGCAGCTGGA AGAGATGCGC AAGCGAGAAG CAGAAGATCG CGCCAGGCAG
    GAGGACGAGC GGCGCCGCCA GGAGGAGGAG AGAGCAAAGC GAGATGCTGA AGAAAAGAGG
    CGTCAGGAAG AAGGCTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG
    GCCGAGCGCA GGTTGCTGGA GCCGGAGGAG CCGGGTCTGT GCAGACCTCC GCTGCCGCGG
    GGCTACAAGC TCCCGTCCCC GTCCCCTCCG GCTGCCGCTC CTCCTCCCCC GCCTCAGCGG
    ACCGCCTCCT CCCTCCAGGC CCAGGCCCTC TCCCCAGACT CCCTGTACAC CGCCAGGCTC
    GTTGCGTACC ACGAGGAGGA GGAGGAGGAG CACTGCAGTC TGGCAGGACC AAACTCTTAC
    CTGGGATCTG CCGGGACAGC TGTTGGCACC CACGATGCCT ATCGGGACCC GAGAGAAAGA
    CTGTCTAAAA GCCACGAGGC AGATTTACCT GGGAGCTCTG GAGCCCCTGA AAACTTGACA
    TTCAAAGAGC GCCAGCGTCT GTTCTCACAA GGTCAGGACG TGTCCAATAA AGTAAAAGCC
    TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274763.1
    CDS42..5426
    Translation

    Target ORF information:

    RefSeq Version XM_022419055.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG
    AAGAATATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAATG CATCTGAGCT GCTCAACTTC
    ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACACTGGACG CCCAGGACGT TTTAGCACAC
    TTGGTTCAGA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC
    ATGCCAGCCT TCCTGGATGA CCCCGAGGAG AACAGCCTAC AGAGACCAAA AATAGATGAT
    GTTTTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTTATCA ACATGTGGCT CTTCAACAGA
    TTGGTGACCG ACCCTGAGTC GGGACTGTGC TCCCACTACT GGGGGGCGAT TATTCGCCAG
    CAGCTAGGAC ACGTGGAAGC TTGGGCAGAG AAGCAGGGGC TGGAGCTGGC AGCGGACTGT
    CACCTCAGCA GGATCGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT
    GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAATT CACTGCAACT TCAAGCCTTG
    TTACAGAATT ACCACTGTGC ACCTGATGAG CCTTTTATTC CCACGGACCT GATAGAGAAT
    GTGGTGGCTG TGGCTGAAAA CACGGCTGAC GAGCTGGCGC GCAGTGATGG CCGGGAGGTG
    CAGCTGGAAG AGGACCCCGA TCTGCAGTTG CCTTTTCTTT TGCCAGAAGA CGGCTATTCT
    TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TTTGTGCCAG
    AGAGGATTTT GCAGGTTAAT CCCTCATGCA CGTTCACCAG GTACTTGGAC AATATATTTT
    GAGGGTGCAG ATTACGAGAG CCATCTTCTG CGTGAGAACA CGGAGCTGGC TCAGCCTCTG
    AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAGAAGC AAAATGGGAT GGGCCTTAGC
    ATCGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTTGTA
    AAAGGCGGTG CTGCGGATGT GGATGGACGC CTGGCTGCGG GTGACCAGCT CCTCAGTGTG
    GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAGCTCAT GACAAGAACA
    AGTTCTGTGG TGACACTAGA AGTAGCAAAG CAAGGAGCAA TTTATCACGG CCTCGCCACG
    CTGCTTAATC AGCCATCCCC AATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA
    CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCAAAA CGGGTCACCT
    GAGAGCCCTC AGCTGCCTTG GGCAGAATAC ACTGAACCCA AGAAATTGCC TGGCGATGAC
    AGACTGATGA AGAACCGGGC TGATCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAAAGCGC ATACGCCCCT GGAACAACAG CTAAGATAAC GTCTGTCTCC
    ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC TTACCCCATC
    CCCACTCAGA CATACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG
    ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACATGGAC
    AATAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAGGAGGA GCTTCGGGAG
    GATAAGGCTT ACCAACTAGA GCGGCATCGG ATCGAGGCAG TCCTGGATCG CAAGTCTGAT
    AGCGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCGA GCACTTCCAG CCAAGAGCAC
    CTGGACCACC CCTCCAAGGC CGGCACCCCC GCTTCCACTC TGACCAAGAG TGGCCCTGGG
    CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC CTGCCTGTGG CTGGCTCCCA GCCGGTCCAA
    ACTGACCTGC CCCCACCACC CCCGCCGCCC CCCACGCACT ACGCCGGAGA CTTCGACGGG
    ATCCCAACAG ACCTGCCTCT CCCACCACCT CCTGCTAGCC AGACAGGACC TGCCTCTGCT
    CAGGTGGTGG CTGCAGAGAG GAAGAAGAGG GAGGAGCACC AGCGCTGGTA TGAGAAGGAG
    AAGGCCCGTC TAGAGGAGGA GCGGGACAGG AAGCGCCGTG AGCAGGAGCG AAAGCTGGGC
    CAGATGCGCT CCCAGTCACT GAACCCAGCC CCGTTTTCTC CTGTAACTGT CCCCCATGTG
    AAGCCAGAAA AACCTTCTAC ACTCCAGAGG CCCCAAGAGA CGGTCATTCG GGAGCTGCAG
    CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC AGTCAGCAAA
    GAGGAGCTGT CCTCAGGAGA TAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCCGGGAG
    AAGCTAGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCAGGAG
    CTCCAGAATA AGCCCGAGCG CAGTGCAGAG GAAAACGATC GCCTGCGGAA ACTCATGCTG
    GAGTGGCAGT TCCAGAAGAG GCTCCAGGAG TCCAAGCAGA AGGACGAGGA TGACGAGGAG
    GAGGAGGATG ATGACGTGGA TACCATGCTC ATCATGCAGC GCCTCGAGGC CGAGCGAAGA
    GCAAGGCAGA CTGCTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA TGAGGAGCGG
    AGGCGCCAGC AGCAGCTGGA AGAGATGCGC AAGCGAGAAG CAGAAGATCG CGCCAGGCAG
    GAGGACGAGC GGCGCCGCCA GGAGGAGGAG AGAGCAAAGC GAGATGCTGA AGAAAAGAGG
    CGTCAGGAAG AAGGCTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG
    GCCGAGCGCA GGTTGCTGGA GCCGGAGGAG CCGGGTCTGT GCAGACCTCC GCTGCCGCGG
    GGCTACAAGC TCCCGTCCCC GTCCCCTCCG GCTGCCGCTC CTCCTCCCCC GCCTCAGCGG
    ACCGCCTCCT CCCTCCAGGC CCAGGCCCTC TCCCCAGACT CCCTGTACAC CGCCAGGCTC
    GTTGCGTACC ACGAGGAGGA GGAGGAGGAG CACTGCAGTC TGGCAGGACC AAACTCTTAC
    CTGGGATCTG CCGGGACAGC TGTTGGCACC CACGATGCCT ATCGGGACCC GAGAGAAAGA
    CTGTCTAAAA GCCACGAGGC AGATTTACCT GGGAGCTCTG GAGCCCCTGA AAACTTGACA
    TTCAAAGAGC GCCAGCGTCT GTTCTCACAA GGTCAGGACG TGTCCAATAA AGTAAAAGCC
    TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285923
    Clone ID Related Accession (Same CDS sequence) XM_022419066.1
    Accession Version XM_022419066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGGAT GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA
    GAGATGCGCA AGCGAGAAGC AGAAGATCGC GCCAGGCAGG AGGACGAGCG GCGCCGCCAG
    GAGGAGGAGA GAGCAAAGCG AGATGCTGAA GAAAAGAGGC GTCAGGAAGA AGGCTATTAC
    AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GTTGCTGGAG
    CCGGAGGAGC CGGGTCTGTG CAGACCTCCG CTGCCGCGGG GCTACAAGCT CCCGTCCCCG
    TCCCCTCCGG CTGCCGCTCC TCCTCCCCCG CCTCAGCGGA CCGCCTCCTC CCTCCAGGCC
    CAGGCCCTCT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG
    GAGGAGGAGC ACTGCAGTCT GGCAGGACCA AACTCTTACC TGGGATCTGC CGGGACAGCT
    GTTGGCACCC ACGATGCCTA TCGGGACCCG AGAGAAAGAC TGTCTAAAAG CCACGAGGCA
    GATTTACCTG GGAGCTCTGG AGCCCCTGAA AACTTGACAT TCAAAGAGCG CCAGCGTCTG
    TTCTCACAAG GTCAGGACGT GTCCAATAAA GTAAAAGCCT CTCGTAAATT AACAGAACTG
    GAAAACGAAC TGAACACCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274774.1
    CDS42..5405
    Translation

    Target ORF information:

    RefSeq Version XM_022419066.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGGAT GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA
    GAGATGCGCA AGCGAGAAGC AGAAGATCGC GCCAGGCAGG AGGACGAGCG GCGCCGCCAG
    GAGGAGGAGA GAGCAAAGCG AGATGCTGAA GAAAAGAGGC GTCAGGAAGA AGGCTATTAC
    AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GTTGCTGGAG
    CCGGAGGAGC CGGGTCTGTG CAGACCTCCG CTGCCGCGGG GCTACAAGCT CCCGTCCCCG
    TCCCCTCCGG CTGCCGCTCC TCCTCCCCCG CCTCAGCGGA CCGCCTCCTC CCTCCAGGCC
    CAGGCCCTCT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG
    GAGGAGGAGC ACTGCAGTCT GGCAGGACCA AACTCTTACC TGGGATCTGC CGGGACAGCT
    GTTGGCACCC ACGATGCCTA TCGGGACCCG AGAGAAAGAC TGTCTAAAAG CCACGAGGCA
    GATTTACCTG GGAGCTCTGG AGCCCCTGAA AACTTGACAT TCAAAGAGCG CCAGCGTCTG
    TTCTCACAAG GTCAGGACGT GTCCAATAAA GTAAAAGCCT CTCGTAAATT AACAGAACTG
    GAAAACGAAC TGAACACCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285924
    Clone ID Related Accession (Same CDS sequence) XM_022419071.1
    Accession Version XM_022419071.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGATGGCT CTGACTCCAG AGATAAGCCC
    AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTTGGGA CGGAAAAGTT TGATGAGAAC
    TCCATCCAGC TGTTCGGATC AGGAATTCAG CCCCACCACT GCGACCTCAC AAACATGGAT
    GGAGTTGTCA CTGTAACGCC GAGAAGCATG GATGCTGAGA CTTACGTGGA AGGACAGCGC
    ATCTCGGAGA CAACCATGCT ACAGAGCGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG
    TTCAAGTTTG TGGATCCCAG CCAGGACCAC GCTCTGGCAA AGCGATCTGT GGATGGAAGC
    CTGACCGCCA AGGGTCCCAG ACATAAACCT GGAATTGTTC AAGAGACAAC ATTTGATTTA
    GGAGGAGATG TTCACAGTGG AACAGCATTA CCAACAAGTA AGAGCACCAC TCGGCTGGAC
    GGCGACAGGG TATTGTCTGC CTCCAGCACA GCGGAGCGAG GGATGGTGAA GCCGATGATG
    AGAGTAGAGC AGCAGCAGGA CTACCGCAGG CAAGAAAACA GAACTCAGGA TGCTCCTGGG
    CCTGAGCTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTA
    TCTGCCATTA TAAACTATAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC ACCTACATAT
    GTATTATATA TGGCATGTCG GTATGTATTG TCAAGCCAGT ACAGACCCGA CGTCAGCCCT
    ACAGAGCGCA CTCACAAAGT GATTGCAATA GTCAACAAGA TGGTGAGCAT GATGGAGGGG
    GTGATTCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ATATTGCAGG GGCCCTTGCC
    TTCTGGATGG CCAATGCATC TGAGCTGCTC AACTTCATTA AGCAGGACCG AGATCTTAGT
    CGAATCACAC TGGACGCCCA GGACGTTTTA GCACACTTGG TTCAGATGGC GTTTAAATAT
    CTGGTTCATT GTCTTCAATC AGAACTGAAT AATTACATGC CAGCCTTCCT GGATGACCCC
    GAGGAGAACA GCCTACAGAG ACCAAAAATA GATGATGTTT TGCACACGCT CACGGGAGCC
    ATGTCTTTGC TGCGGCGCTG CAGAGTCAAT GCTGCCCTGA CCATCCAGCT CTTCTCTCAG
    CTCTTCCACT TTATCAACAT GTGGCTCTTC AACAGATTGG TGACCGACCC TGAGTCGGGA
    CTGTGCTCCC ACTACTGGGG GGCGATTATT CGCCAGCAGC TAGGACACGT GGAAGCTTGG
    GCAGAGAAGC AGGGGCTGGA GCTGGCAGCG GACTGTCACC TCAGCAGGAT CGTGCAGGCA
    ACCACTTTGC TTACAATGGA TAAATACGCA CCTGATGACA TTCCAAATAT AAACAGTACC
    TGCTTTAAGT TAAATTCACT GCAACTTCAA GCCTTGTTAC AGAATTACCA CTGTGCACCT
    GATGAGCCTT TTATTCCCAC GGACCTGATA GAGAATGTGG TGGCTGTGGC TGAAAACACG
    GCTGACGAGC TGGCGCGCAG TGATGGCCGG GAGGTGCAGC TGGAAGAGGA CCCCGATCTG
    CAGTTGCCTT TTCTTTTGCC AGAAGACGGC TATTCTTGTG ATGTTGTCAG GAACATTCCA
    AATGGTTTAC AAGAATTTTT AGACCCTTTG TGCCAGAGAG GATTTTGCAG GTTAATCCCT
    CATGCACGTT CACCAGGTAC TTGGACAATA TATTTTGAGG GTGCAGATTA CGAGAGCCAT
    CTTCTGCGTG AGAACACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCTGA AATAATCACT
    GTGACTCTTA AGAAGCAAAA TGGGATGGGC CTTAGCATCG TTGCAGCAAA GGGTGCTGGT
    CAAGATAAAC TTGGAATCTA TGTCAAGTCT GTTGTAAAAG GCGGTGCTGC GGATGTGGAT
    GGACGCCTGG CTGCGGGTGA CCAGCTCCTC AGTGTGGACG GACGAAGTCT GGTAGGACTC
    TCTCAAGAAA GGGCGGCAGA GCTCATGACA AGAACAAGTT CTGTGGTGAC ACTAGAAGTA
    GCAAAGCAAG GAGCAATTTA TCACGGCCTC GCCACGCTGC TTAATCAGCC ATCCCCAATG
    ATGCAAAGAA TTTCAGACCG TCGTGGCTCA GGTAAACCCC GACCAAAGAG TGAAGGTTTC
    GAGCTCTATA ATAATTCAGC TCAAAACGGG TCACCTGAGA GCCCTCAGCT GCCTTGGGCA
    GAATACACTG AACCCAAGAA ATTGCCTGGC GATGACAGAC TGATGAAGAA CCGGGCTGAT
    CACCGCTCCA GCCCCAACGT GGCAAATCAG CCTCCTAGCC CTGGAGGGAA AAGCGCATAC
    GCCCCTGGAA CAACAGCTAA GATAACGTCT GTCTCCACTG GGAACCTCTG CACTGAGGAA
    CAGACCCCTC CACCTAGACC TGAGGCTTAC CCCATCCCCA CTCAGACATA CACCAGAGAG
    TATTTCACCT TCCCAGCTTC CAAATCCCAG GACCGGATGG CTCCTCCTCA GAACCAGTGG
    CCAAATTACG AGGAGAAGCC ACACATGCAC ATGGACAATA ATCATTCCAG TATTGCAATT
    CAGCGTGTTA CCCGTTCCCA GGAGGAGCTT CGGGAGGATA AGGCTTACCA ACTAGAGCGG
    CATCGGATCG AGGCAGTCCT GGATCGCAAG TCTGATAGCG ATATGTGGAT CCATCAGAGC
    TCCTCAGTGG GCTCGAGCAC TTCCAGCCAA GAGCACCTGG ACCACCCCTC CAAGGCCGGC
    ACCCCCGCTT CCACTCTGAC CAAGAGTGGC CCTGGGCGCT GGAAGACGCC AGCCGCTGTG
    CAGCCCCTGC CTGTGGCTGG CTCCCAGCCG GTCCAAACTG ACCTGCCCCC ACCACCCCCG
    CCGCCCCCCA CGCACTACGC CGGAGACTTC GACGGGATCC CAACAGACCT GCCTCTCCCA
    CCACCTCCTG CTAGCCAGAC AGGACCTGCC TCTGCTCAGG TGGTGGCTGC AGAGAGGAAG
    AAGAGGGAGG AGCACCAGCG CTGGTATGAG AAGGAGAAGG CCCGTCTAGA GGAGGAGCGG
    GACAGGAAGC GCCGTGAGCA GGAGCGAAAG CTGGGCCAGA TGCGCTCCCA GTCACTGAAC
    CCAGCCCCGT TTTCTCCTGT AACTGTCCCC CATGTGAAGC CAGAAAAACC TTCTACACTC
    CAGAGGCCCC AAGAGACGGT CATTCGGGAG CTGCAGCCCC AGCAGCAGCC CCGCACGATT
    GAGCGCAGAG ACTTACAGTA CATTACAGTC AGCAAAGAGG AGCTGTCCTC AGGAGATAGT
    CTGTCCCCAG ACCCGTGGAA GCGGGATGCC CGGGAGAAGC TAGAGAAGCA GCAGCAGATG
    CACATCGTGG ATATGCTGAG CAGGGAGATC CAGGAGCTCC AGAATAAGCC CGAGCGCAGT
    GCAGAGGAAA ACGATCGCCT GCGGAAACTC ATGCTGGAGT GGCAGTTCCA GAAGAGGCTC
    CAGGAGTCCA AGCAGAAGGA CGAGGATGAC GAGGAGGAGG AGGATGATGA CGTGGATACC
    ATGCTCATCA TGCAGCGCCT CGAGGCCGAG CGAAGAGCAA GGCAGACTGC TCTGCCAGCA
    ATCTCTGTTC TAGATTTGTT ACAGGATGAG GAGCGGAGGC GCCAGCAGCA GCTGGAAGAG
    ATGCGCAAGC GAGAAGCAGA AGATCGCGCC AGGCAGGAGG ACGAGCGGCG CCGCCAGGAG
    GAGGAGAGAG CAAAGCGAGA TGCTGAAGAA AAGAGGCGTC AGGAAGAAGG CTATTACAGC
    CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGTT GCTGGAGCCG
    GAGGAGCCGG GTCTGTGCAG ACCTCCGCTG CCGCGGGGCT ACAAGCTCCC GTCCCCGTCC
    CCTCCGGCTG CCGCTCCTCC TCCCCCGCCT CAGCGGACCG CCTCCTCCCT CCAGGCCCAG
    GCCCTCTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG
    GAGGAGCACT GCAGTCTGGC AGGACCAAAC TCTTACCTGG GATCTGCCGG GACAGCTGTT
    GGCACCCACG ATGCCTATCG GGACCCGAGA GAAAGACTGT CTAAAAGCCA CGAGGCAGAT
    TTACCTGGGA GCTCTGGAGC CCCTGAAAAC TTGACATTCA AAGAGCGCCA GCGTCTGTTC
    TCACAAGGTC AGGACGTGTC CAATAAAGTA AAAGCCTCTC GTAAATTAAC AGAACTGGAA
    AACGAACTGA ACACCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274779.1
    CDS42..5402
    Translation

    Target ORF information:

    RefSeq Version XM_022419071.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419071.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGATGGCT CTGACTCCAG AGATAAGCCC
    AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTTGGGA CGGAAAAGTT TGATGAGAAC
    TCCATCCAGC TGTTCGGATC AGGAATTCAG CCCCACCACT GCGACCTCAC AAACATGGAT
    GGAGTTGTCA CTGTAACGCC GAGAAGCATG GATGCTGAGA CTTACGTGGA AGGACAGCGC
    ATCTCGGAGA CAACCATGCT ACAGAGCGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG
    TTCAAGTTTG TGGATCCCAG CCAGGACCAC GCTCTGGCAA AGCGATCTGT GGATGGAAGC
    CTGACCGCCA AGGGTCCCAG ACATAAACCT GGAATTGTTC AAGAGACAAC ATTTGATTTA
    GGAGGAGATG TTCACAGTGG AACAGCATTA CCAACAAGTA AGAGCACCAC TCGGCTGGAC
    GGCGACAGGG TATTGTCTGC CTCCAGCACA GCGGAGCGAG GGATGGTGAA GCCGATGATG
    AGAGTAGAGC AGCAGCAGGA CTACCGCAGG CAAGAAAACA GAACTCAGGA TGCTCCTGGG
    CCTGAGCTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTA
    TCTGCCATTA TAAACTATAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC ACCTACATAT
    GTATTATATA TGGCATGTCG GTATGTATTG TCAAGCCAGT ACAGACCCGA CGTCAGCCCT
    ACAGAGCGCA CTCACAAAGT GATTGCAATA GTCAACAAGA TGGTGAGCAT GATGGAGGGG
    GTGATTCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ATATTGCAGG GGCCCTTGCC
    TTCTGGATGG CCAATGCATC TGAGCTGCTC AACTTCATTA AGCAGGACCG AGATCTTAGT
    CGAATCACAC TGGACGCCCA GGACGTTTTA GCACACTTGG TTCAGATGGC GTTTAAATAT
    CTGGTTCATT GTCTTCAATC AGAACTGAAT AATTACATGC CAGCCTTCCT GGATGACCCC
    GAGGAGAACA GCCTACAGAG ACCAAAAATA GATGATGTTT TGCACACGCT CACGGGAGCC
    ATGTCTTTGC TGCGGCGCTG CAGAGTCAAT GCTGCCCTGA CCATCCAGCT CTTCTCTCAG
    CTCTTCCACT TTATCAACAT GTGGCTCTTC AACAGATTGG TGACCGACCC TGAGTCGGGA
    CTGTGCTCCC ACTACTGGGG GGCGATTATT CGCCAGCAGC TAGGACACGT GGAAGCTTGG
    GCAGAGAAGC AGGGGCTGGA GCTGGCAGCG GACTGTCACC TCAGCAGGAT CGTGCAGGCA
    ACCACTTTGC TTACAATGGA TAAATACGCA CCTGATGACA TTCCAAATAT AAACAGTACC
    TGCTTTAAGT TAAATTCACT GCAACTTCAA GCCTTGTTAC AGAATTACCA CTGTGCACCT
    GATGAGCCTT TTATTCCCAC GGACCTGATA GAGAATGTGG TGGCTGTGGC TGAAAACACG
    GCTGACGAGC TGGCGCGCAG TGATGGCCGG GAGGTGCAGC TGGAAGAGGA CCCCGATCTG
    CAGTTGCCTT TTCTTTTGCC AGAAGACGGC TATTCTTGTG ATGTTGTCAG GAACATTCCA
    AATGGTTTAC AAGAATTTTT AGACCCTTTG TGCCAGAGAG GATTTTGCAG GTTAATCCCT
    CATGCACGTT CACCAGGTAC TTGGACAATA TATTTTGAGG GTGCAGATTA CGAGAGCCAT
    CTTCTGCGTG AGAACACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCTGA AATAATCACT
    GTGACTCTTA AGAAGCAAAA TGGGATGGGC CTTAGCATCG TTGCAGCAAA GGGTGCTGGT
    CAAGATAAAC TTGGAATCTA TGTCAAGTCT GTTGTAAAAG GCGGTGCTGC GGATGTGGAT
    GGACGCCTGG CTGCGGGTGA CCAGCTCCTC AGTGTGGACG GACGAAGTCT GGTAGGACTC
    TCTCAAGAAA GGGCGGCAGA GCTCATGACA AGAACAAGTT CTGTGGTGAC ACTAGAAGTA
    GCAAAGCAAG GAGCAATTTA TCACGGCCTC GCCACGCTGC TTAATCAGCC ATCCCCAATG
    ATGCAAAGAA TTTCAGACCG TCGTGGCTCA GGTAAACCCC GACCAAAGAG TGAAGGTTTC
    GAGCTCTATA ATAATTCAGC TCAAAACGGG TCACCTGAGA GCCCTCAGCT GCCTTGGGCA
    GAATACACTG AACCCAAGAA ATTGCCTGGC GATGACAGAC TGATGAAGAA CCGGGCTGAT
    CACCGCTCCA GCCCCAACGT GGCAAATCAG CCTCCTAGCC CTGGAGGGAA AAGCGCATAC
    GCCCCTGGAA CAACAGCTAA GATAACGTCT GTCTCCACTG GGAACCTCTG CACTGAGGAA
    CAGACCCCTC CACCTAGACC TGAGGCTTAC CCCATCCCCA CTCAGACATA CACCAGAGAG
    TATTTCACCT TCCCAGCTTC CAAATCCCAG GACCGGATGG CTCCTCCTCA GAACCAGTGG
    CCAAATTACG AGGAGAAGCC ACACATGCAC ATGGACAATA ATCATTCCAG TATTGCAATT
    CAGCGTGTTA CCCGTTCCCA GGAGGAGCTT CGGGAGGATA AGGCTTACCA ACTAGAGCGG
    CATCGGATCG AGGCAGTCCT GGATCGCAAG TCTGATAGCG ATATGTGGAT CCATCAGAGC
    TCCTCAGTGG GCTCGAGCAC TTCCAGCCAA GAGCACCTGG ACCACCCCTC CAAGGCCGGC
    ACCCCCGCTT CCACTCTGAC CAAGAGTGGC CCTGGGCGCT GGAAGACGCC AGCCGCTGTG
    CAGCCCCTGC CTGTGGCTGG CTCCCAGCCG GTCCAAACTG ACCTGCCCCC ACCACCCCCG
    CCGCCCCCCA CGCACTACGC CGGAGACTTC GACGGGATCC CAACAGACCT GCCTCTCCCA
    CCACCTCCTG CTAGCCAGAC AGGACCTGCC TCTGCTCAGG TGGTGGCTGC AGAGAGGAAG
    AAGAGGGAGG AGCACCAGCG CTGGTATGAG AAGGAGAAGG CCCGTCTAGA GGAGGAGCGG
    GACAGGAAGC GCCGTGAGCA GGAGCGAAAG CTGGGCCAGA TGCGCTCCCA GTCACTGAAC
    CCAGCCCCGT TTTCTCCTGT AACTGTCCCC CATGTGAAGC CAGAAAAACC TTCTACACTC
    CAGAGGCCCC AAGAGACGGT CATTCGGGAG CTGCAGCCCC AGCAGCAGCC CCGCACGATT
    GAGCGCAGAG ACTTACAGTA CATTACAGTC AGCAAAGAGG AGCTGTCCTC AGGAGATAGT
    CTGTCCCCAG ACCCGTGGAA GCGGGATGCC CGGGAGAAGC TAGAGAAGCA GCAGCAGATG
    CACATCGTGG ATATGCTGAG CAGGGAGATC CAGGAGCTCC AGAATAAGCC CGAGCGCAGT
    GCAGAGGAAA ACGATCGCCT GCGGAAACTC ATGCTGGAGT GGCAGTTCCA GAAGAGGCTC
    CAGGAGTCCA AGCAGAAGGA CGAGGATGAC GAGGAGGAGG AGGATGATGA CGTGGATACC
    ATGCTCATCA TGCAGCGCCT CGAGGCCGAG CGAAGAGCAA GGCAGACTGC TCTGCCAGCA
    ATCTCTGTTC TAGATTTGTT ACAGGATGAG GAGCGGAGGC GCCAGCAGCA GCTGGAAGAG
    ATGCGCAAGC GAGAAGCAGA AGATCGCGCC AGGCAGGAGG ACGAGCGGCG CCGCCAGGAG
    GAGGAGAGAG CAAAGCGAGA TGCTGAAGAA AAGAGGCGTC AGGAAGAAGG CTATTACAGC
    CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGTT GCTGGAGCCG
    GAGGAGCCGG GTCTGTGCAG ACCTCCGCTG CCGCGGGGCT ACAAGCTCCC GTCCCCGTCC
    CCTCCGGCTG CCGCTCCTCC TCCCCCGCCT CAGCGGACCG CCTCCTCCCT CCAGGCCCAG
    GCCCTCTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG
    GAGGAGCACT GCAGTCTGGC AGGACCAAAC TCTTACCTGG GATCTGCCGG GACAGCTGTT
    GGCACCCACG ATGCCTATCG GGACCCGAGA GAAAGACTGT CTAAAAGCCA CGAGGCAGAT
    TTACCTGGGA GCTCTGGAGC CCCTGAAAAC TTGACATTCA AAGAGCGCCA GCGTCTGTTC
    TCACAAGGTC AGGACGTGTC CAATAAAGTA AAAGCCTCTC GTAAATTAAC AGAACTGGAA
    AACGAACTGA ACACCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285925
    Clone ID Related Accession (Same CDS sequence) XM_022419076.1
    Accession Version XM_022419076.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAAATCAG
    CCTCCTAGCC CTGGAGGGAA AAGCGCATAC GCCCCTGGAA CAACAGCTAA GATAACGTCT
    GTCTCCACTG GGAACCTCTG CACTGAGGAA CAGACCCCTC CACCTAGACC TGAGGCTTAC
    CCCATCCCCA CTCAGACATA CACCAGAGAG TATTTCACCT TCCCAGCTTC CAAATCCCAG
    GACCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTACG AGGAGAAGCC ACACATGCAC
    ATGGACAATA ATCATTCCAG TATTGCAATT CAGCGTGTTA CCCGTTCCCA GGAGGAGCTT
    CGGGAGGATA AGGCTTACCA ACTAGAGCGG CATCGGATCG AGGCAGTCCT GGATCGCAAG
    TCTGATAGCG ATATGTGGAT CCATCAGAGC TCCTCAGTGG GCTCGAGCAC TTCCAGCCAA
    GAGCACCTGG ACCACCCCTC CAAGGCCGGC ACCCCCGCTT CCACTCTGAC CAAGAGTGGC
    CCTGGGCGCT GGAAGACGCC AGCCGCTGTG CAGCCCCTGC CTGTGGCTGG CTCCCAGCCG
    GTCCAAACTG ACCTGCCCCC ACCACCCCCG CCGCCCCCCA CGCACTACGC CGGAGACTTC
    GACGGGATCC CAACAGACCT GCCTCTCCCA CCACCTCCTG CTAGCCAGAC AGGACCTGCC
    TCTGCTCAGG TGGTGGCTGC AGAGAGGAAG AAGAGGGAGG AGCACCAGCG CTGGTATGAG
    AAGGAGAAGG CCCGTCTAGA GGAGGAGCGG GACAGGAAGC GCCGTGAGCA GGAGCGAAAG
    CTGGGCCAGA TGCGCTCCCA GTCACTGAAC CCAGCCCCGT TTTCTCCTGT AACTGTCCCC
    CATGTGAAGC CAGAAAAACC TTCTACACTC CAGAGGCCCC AAGAGACGGT CATTCGGGAG
    CTGCAGCCCC AGCAGCAGCC CCGCACGATT GAGCGCAGAG ACTTACAGTA CATTACAGTC
    AGCAAAGAGG AGCTGTCCTC AGGAGATAGT CTGTCCCCAG ACCCGTGGAA GCGGGATGCC
    CGGGAGAAGC TAGAGAAGCA GCAGCAGATG CACATCGTGG ATATGCTGAG CAGGGAGATC
    CAGGAGCTCC AGAATAAGCC CGAGCGCAGT GCAGAGGAAA ACGATCGCCT GCGGAAACTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGGCTC CAGGAGTCCA AGCAGAAGGA CGAGGATGAC
    GAGGAGGAGG AGGATGATGA CGTGGATACC ATGCTCATCA TGCAGCGCCT CGAGGCCGAG
    CGAAGAGCAA GGCAGACTGC TCTGCCAGCA ATCTCTGTTC TAGATTTGTT ACAGGATGAG
    GAGCGGAGGC GCCAGCAGCA GCTGGAAGAG ATGCGCAAGC GAGAAGCAGA AGATCGCGCC
    AGGCAGGAGG ACGAGCGGCG CCGCCAGGAG GAGGAGAGAG CAAAGCGAGA TGCTGAAGAA
    AAGAGGCGTC AGGAAGAAGG CTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC
    GCGGAGGCCG AGCGCAGGTT GCTGGAGCCG GAGGAGCCGG GTCTGTGCAG ACCTCCGCTG
    CCGCGGGGCT ACAAGCTCCC GTCCCCGTCC CCTCCGGCTG CCGCTCCTCC TCCCCCGCCT
    CAGCGGACCG CCTCCTCCCT CCAGGCCCAG GCCCTCTCCC CAGACTCCCT GTACACCGCC
    AGGCTCGTTG CGTACCACGA GGAGGAGGAG GAGGAGCACT GCAGTCTGGC AGGACCAAAC
    TCTTACCTGG GATCTGCCGG GACAGCTGTT GGCACCCACG ATGCCTATCG GGACCCGAGA
    GAAAGACTGT CTAAAAGCCA CGAGGCAGAT TTACCTGGGA GCTCTGGAGC CCCTGAAAAC
    TTGACATTCA AAGAGCGCCA GCGTCTGTTC TCACAAGGTC AGGACGTGTC CAATAAAGTA
    AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274784.1
    CDS43..5253
    Translation

    Target ORF information:

    RefSeq Version XM_022419076.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAAATCAG
    CCTCCTAGCC CTGGAGGGAA AAGCGCATAC GCCCCTGGAA CAACAGCTAA GATAACGTCT
    GTCTCCACTG GGAACCTCTG CACTGAGGAA CAGACCCCTC CACCTAGACC TGAGGCTTAC
    CCCATCCCCA CTCAGACATA CACCAGAGAG TATTTCACCT TCCCAGCTTC CAAATCCCAG
    GACCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTACG AGGAGAAGCC ACACATGCAC
    ATGGACAATA ATCATTCCAG TATTGCAATT CAGCGTGTTA CCCGTTCCCA GGAGGAGCTT
    CGGGAGGATA AGGCTTACCA ACTAGAGCGG CATCGGATCG AGGCAGTCCT GGATCGCAAG
    TCTGATAGCG ATATGTGGAT CCATCAGAGC TCCTCAGTGG GCTCGAGCAC TTCCAGCCAA
    GAGCACCTGG ACCACCCCTC CAAGGCCGGC ACCCCCGCTT CCACTCTGAC CAAGAGTGGC
    CCTGGGCGCT GGAAGACGCC AGCCGCTGTG CAGCCCCTGC CTGTGGCTGG CTCCCAGCCG
    GTCCAAACTG ACCTGCCCCC ACCACCCCCG CCGCCCCCCA CGCACTACGC CGGAGACTTC
    GACGGGATCC CAACAGACCT GCCTCTCCCA CCACCTCCTG CTAGCCAGAC AGGACCTGCC
    TCTGCTCAGG TGGTGGCTGC AGAGAGGAAG AAGAGGGAGG AGCACCAGCG CTGGTATGAG
    AAGGAGAAGG CCCGTCTAGA GGAGGAGCGG GACAGGAAGC GCCGTGAGCA GGAGCGAAAG
    CTGGGCCAGA TGCGCTCCCA GTCACTGAAC CCAGCCCCGT TTTCTCCTGT AACTGTCCCC
    CATGTGAAGC CAGAAAAACC TTCTACACTC CAGAGGCCCC AAGAGACGGT CATTCGGGAG
    CTGCAGCCCC AGCAGCAGCC CCGCACGATT GAGCGCAGAG ACTTACAGTA CATTACAGTC
    AGCAAAGAGG AGCTGTCCTC AGGAGATAGT CTGTCCCCAG ACCCGTGGAA GCGGGATGCC
    CGGGAGAAGC TAGAGAAGCA GCAGCAGATG CACATCGTGG ATATGCTGAG CAGGGAGATC
    CAGGAGCTCC AGAATAAGCC CGAGCGCAGT GCAGAGGAAA ACGATCGCCT GCGGAAACTC
    ATGCTGGAGT GGCAGTTCCA GAAGAGGCTC CAGGAGTCCA AGCAGAAGGA CGAGGATGAC
    GAGGAGGAGG AGGATGATGA CGTGGATACC ATGCTCATCA TGCAGCGCCT CGAGGCCGAG
    CGAAGAGCAA GGCAGACTGC TCTGCCAGCA ATCTCTGTTC TAGATTTGTT ACAGGATGAG
    GAGCGGAGGC GCCAGCAGCA GCTGGAAGAG ATGCGCAAGC GAGAAGCAGA AGATCGCGCC
    AGGCAGGAGG ACGAGCGGCG CCGCCAGGAG GAGGAGAGAG CAAAGCGAGA TGCTGAAGAA
    AAGAGGCGTC AGGAAGAAGG CTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC
    GCGGAGGCCG AGCGCAGGTT GCTGGAGCCG GAGGAGCCGG GTCTGTGCAG ACCTCCGCTG
    CCGCGGGGCT ACAAGCTCCC GTCCCCGTCC CCTCCGGCTG CCGCTCCTCC TCCCCCGCCT
    CAGCGGACCG CCTCCTCCCT CCAGGCCCAG GCCCTCTCCC CAGACTCCCT GTACACCGCC
    AGGCTCGTTG CGTACCACGA GGAGGAGGAG GAGGAGCACT GCAGTCTGGC AGGACCAAAC
    TCTTACCTGG GATCTGCCGG GACAGCTGTT GGCACCCACG ATGCCTATCG GGACCCGAGA
    GAAAGACTGT CTAAAAGCCA CGAGGCAGAT TTACCTGGGA GCTCTGGAGC CCCTGAAAAC
    TTGACATTCA AAGAGCGCCA GCGTCTGTTC TCACAAGGTC AGGACGTGTC CAATAAAGTA
    AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285926
    Clone ID Related Accession (Same CDS sequence) XM_022419083.1
    Accession Version XM_022419083.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGCAGAC TGCTCTGCCA GCAATCTCTG TTCTAGATTT GTTACAGGAT
    GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA GAGATGCGCA AGCGAGAAGC AGAAGATCGC
    GCCAGGCAGG AGGACGAGCG GCGCCGCCAG GAGGAGGAGA GAGCAAAGCG AGATGCTGAA
    GAAAAGAGGC GTCAGGAAGA AGGCTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGCGGAGG CCGAGCGCAG GTTGCTGGAG CCGGAGGAGC CGGGTCTGTG CAGACCTCCG
    CTGCCGCGGG GCTACAAGCT CCCGTCCCCG TCCCCTCCGG CTGCCGCTCC TCCTCCCCCG
    CCTCAGCGGA CCGCCTCCTC CCTCCAGGCC CAGGCCCTCT CCCCAGACTC CCTGTACACC
    GCCAGGCTCG TTGCGTACCA CGAGGAGGAG GAGGAGGAGC ACTGCAGTCT GGCAGGACCA
    AACTCTTACC TGGGATCTGC CGGGACAGCT GTTGGCACCC ACGATGCCTA TCGGGACCCG
    AGAGAAAGAC TGTCTAAAAG CCACGAGGCA GATTTACCTG GGAGCTCTGG AGCCCCTGAA
    AACTTGACAT TCAAAGAGCG CCAGCGTCTG TTCTCACAAG GTCAGGACGT GTCCAATAAA
    GTAAAAGCCT CTCGTAAATT AACAGAACTG GAAAACGAAC TGAACACCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274791.1
    CDS22..5355
    Translation

    Target ORF information:

    RefSeq Version XM_022419083.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGCAGAC TGCTCTGCCA GCAATCTCTG TTCTAGATTT GTTACAGGAT
    GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA GAGATGCGCA AGCGAGAAGC AGAAGATCGC
    GCCAGGCAGG AGGACGAGCG GCGCCGCCAG GAGGAGGAGA GAGCAAAGCG AGATGCTGAA
    GAAAAGAGGC GTCAGGAAGA AGGCTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGCGGAGG CCGAGCGCAG GTTGCTGGAG CCGGAGGAGC CGGGTCTGTG CAGACCTCCG
    CTGCCGCGGG GCTACAAGCT CCCGTCCCCG TCCCCTCCGG CTGCCGCTCC TCCTCCCCCG
    CCTCAGCGGA CCGCCTCCTC CCTCCAGGCC CAGGCCCTCT CCCCAGACTC CCTGTACACC
    GCCAGGCTCG TTGCGTACCA CGAGGAGGAG GAGGAGGAGC ACTGCAGTCT GGCAGGACCA
    AACTCTTACC TGGGATCTGC CGGGACAGCT GTTGGCACCC ACGATGCCTA TCGGGACCCG
    AGAGAAAGAC TGTCTAAAAG CCACGAGGCA GATTTACCTG GGAGCTCTGG AGCCCCTGAA
    AACTTGACAT TCAAAGAGCG CCAGCGTCTG TTCTCACAAG GTCAGGACGT GTCCAATAAA
    GTAAAAGCCT CTCGTAAATT AACAGAACTG GAAAACGAAC TGAACACCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34487
    Clone ID Related Accession (Same CDS sequence) XM_014113172.1
    Accession Version XM_014113172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGTCAGG ACAAGCACTC TGGCACGAGG AAGTCCCACG GGCACCTTCT TCCCGCCGCC
    CTTGAGCCGC AGCGGCCCCC CCGCCCGCCG CGCCCGGCCT CCGACGGCAT CTTTCTCTGC
    ACCTCATTCC GGCCCCCCTT GGCCCGAGCC AACAGTACTG CTCACAAAGC AGGCCAGCCC
    CCACCCCCTC CAAAAAAACC AGGCTTCTAC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT
    TACCTGGGAT CTGCCGGGAC AGCTGTTGGC ACCCACGATG CCTATCGGGA CCCGAGAGAA
    AGACTGTCTA AAAGCCACGA GGCAGATTTA CCTGGGAGCT CTGGAGCCCC TGAAAACTTG
    ACATTCAAAG AGCGCCAGCG TCTGTTCTCA CAAGGTCAGG ACGTGTCCAA TAAAGTAAAA
    GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968647.1
    CDS28..5655
    Translation

    Target ORF information:

    RefSeq Version XM_014113172.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGTCAGG ACAAGCACTC TGGCACGAGG AAGTCCCACG GGCACCTTCT TCCCGCCGCC
    CTTGAGCCGC AGCGGCCCCC CCGCCCGCCG CGCCCGGCCT CCGACGGCAT CTTTCTCTGC
    ACCTCATTCC GGCCCCCCTT GGCCCGAGCC AACAGTACTG CTCACAAAGC AGGCCAGCCC
    CCACCCCCTC CAAAAAAACC AGGCTTCTAC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT
    TACCTGGGAT CTGCCGGGAC AGCTGTTGGC ACCCACGATG CCTATCGGGA CCCGAGAGAA
    AGACTGTCTA AAAGCCACGA GGCAGATTTA CCTGGGAGCT CTGGAGCCCC TGAAAACTTG
    ACATTCAAAG AGCGCCAGCG TCTGTTCTCA CAAGGTCAGG ACGTGTCCAA TAAAGTAAAA
    GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34488
    Clone ID Related Accession (Same CDS sequence) XM_014113176.1
    Accession Version XM_014113176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGTCAGG ACAAGCACTC TGGCACGAGG AAGTCCCACG GGCACCTTCT TCCCGCCGCC
    CTTGAGCCGC AGCGGCCCCC CCGCCCGCCG CGCCCGGCCT CCGACGGCAT CTTTCTCTGC
    ACCTCATTCC GGCCCCCCTT GGCCCGAGCC AACAGTACTG CTCACAAAGC AGGCCAGCCC
    CCACCCCCTC CAAAAAAACC AGGCTTCTAC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT
    TACCTGGGAT CTGCCGGGAC AGCTGTTGGC ACCCACGATG CCTATCGGGA CCCGAGAGAA
    AGACTGTCTA AAAGCCACGA GGCAGATTTA CCTGGGAGCT CTGGAGCCCC TGAAAACTTG
    ACATTCAAAG AGCGCCAGCG TCTGTTCTCA CAAGGTCAGG ACGTGTCCAA TAAAGTAAAA
    GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968651.1
    CDS28..5655
    Translation

    Target ORF information:

    RefSeq Version XM_014113176.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGTCAGG ACAAGCACTC TGGCACGAGG AAGTCCCACG GGCACCTTCT TCCCGCCGCC
    CTTGAGCCGC AGCGGCCCCC CCGCCCGCCG CGCCCGGCCT CCGACGGCAT CTTTCTCTGC
    ACCTCATTCC GGCCCCCCTT GGCCCGAGCC AACAGTACTG CTCACAAAGC AGGCCAGCCC
    CCACCCCCTC CAAAAAAACC AGGCTTCTAC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT
    TACCTGGGAT CTGCCGGGAC AGCTGTTGGC ACCCACGATG CCTATCGGGA CCCGAGAGAA
    AGACTGTCTA AAAGCCACGA GGCAGATTTA CCTGGGAGCT CTGGAGCCCC TGAAAACTTG
    ACATTCAAAG AGCGCCAGCG TCTGTTCTCA CAAGGTCAGG ACGTGTCCAA TAAAGTAAAA
    GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34489
    Clone ID Related Accession (Same CDS sequence) XM_014113178.1
    Accession Version XM_014113178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGTCAGG ACAAGCACTC TGGCACGAGG AAGTCCCACG GGCACCTTCT TCCCGCCGCC
    CTTGAGCCGC AGCGGCCCCC CCGCCCGCCG CGCCCGGCCT CCGACGGCAT CTTTCTCTGC
    ACCTCATTCC GGCCCCCCTT GGCCCGAGCC AACAGTACTG CTCACAAAGC AGGCCAGCCC
    CCACCCCCTC CAAAAAAACC AGGCTTCTAC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT
    TACCTGGGAT CTGCCGGGAC AGCTGTTGGC ACCCACGATG CCTATCGGGA CCCGAGAGAA
    AGACTGTCTA AAAGCCACGA GGCAGATTTA CCTGGGAGCT CTGGAGCCCC TGAAAACTTG
    ACATTCAAAG AGCGCCAGCG TCTGTTCTCA CAAGGTCAGG ACGTGTCCAA TAAAGTAAAA
    GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968653.1
    CDS28..5655
    Translation

    Target ORF information:

    RefSeq Version XM_014113178.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC
    CAGGTTGATC AGAAACAGAA GAATATTGCA GGGGCCCTTG CCTTCTGGAT GGCCAATGCA
    TCTGAGCTGC TCAACTTCAT TAAGCAGGAC CGAGATCTTA GTCGAATCAC ACTGGACGCC
    CAGGACGTTT TAGCACACTT GGTTCAGATG GCGTTTAAAT ATCTGGTTCA TTGTCTTCAA
    TCAGAACTGA ATAATTACAT GCCAGCCTTC CTGGATGACC CCGAGGAGAA CAGCCTACAG
    AGACCAAAAA TAGATGATGT TTTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC
    TGCAGAGTCA ATGCTGCCCT GACCATCCAG CTCTTCTCTC AGCTCTTCCA CTTTATCAAC
    ATGTGGCTCT TCAACAGATT GGTGACCGAC CCTGAGTCGG GACTGTGCTC CCACTACTGG
    GGGGCGATTA TTCGCCAGCA GCTAGGACAC GTGGAAGCTT GGGCAGAGAA GCAGGGGCTG
    GAGCTGGCAG CGGACTGTCA CCTCAGCAGG ATCGTGCAGG CAACCACTTT GCTTACAATG
    GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAATTCA
    CTGCAACTTC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CTGATGAGCC TTTTATTCCC
    ACGGACCTGA TAGAGAATGT GGTGGCTGTG GCTGAAAACA CGGCTGACGA GCTGGCGCGC
    AGTGATGGCC GGGAGGTGCA GCTGGAAGAG GACCCCGATC TGCAGTTGCC TTTTCTTTTG
    CCAGAAGACG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT
    TTAGACCCTT TGTGCCAGAG AGGATTTTGC AGGTTAATCC CTCATGCACG TTCACCAGGT
    ACTTGGACAA TATATTTTGA GGGTGCAGAT TACGAGAGCC ATCTTCTGCG TGAGAACACG
    GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAGAAGCAA
    AATGGGATGG GCCTTAGCAT CGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTTGGAATC
    TATGTCAAGT CTGTTGTAAA AGGCGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCGGGT
    GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA
    GAGCTCATGA CAAGAACAAG TTCTGTGGTG ACACTAGAAG TAGCAAAGCA AGGAGCAATT
    TATCACGGCC TCGCCACGCT GCTTAATCAG CCATCCCCAA TGATGCAAAG AATTTCAGAC
    CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA
    GCTCAAAACG GGTCACCTGA GAGCCCTCAG CTGCCTTGGG CAGAATACAC TGAACCCAAG
    AAATTGCCTG GCGATGACAG ACTGATGAAG AACCGGGCTG ATCACCGCTC CAGCCCCAAC
    GTGGCAAATC AGCCTCCTAG CCCTGGAGGG AAAAGCGCAT ACGCCCCTGG AACAACAGCT
    AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA
    CCTGAGGCTT ACCCCATCCC CACTCAGACA TACACCAGAG AGTATTTCAC CTTCCCAGCT
    TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG
    CCACACATGC ACATGGACAA TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC
    CAGGAGGAGC TTCGGGAGGA TAAGGCTTAC CAACTAGAGC GGCATCGGAT CGAGGCAGTC
    CTGGATCGCA AGTCTGATAG CGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCGAGC
    ACTTCCAGCC AAGAGCACCT GGACCACCCC TCCAAGGCCG GCACCCCCGC TTCCACTCTG
    ACCAAGAGTG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCCT GCCTGTGGCT
    GGCTCCCAGC CGGTCCAAAC TGACCTGCCC CCACCACCCC CGCCGCCCCC CACGCACTAC
    GCCGGAGACT TCGACGGGAT CCCAACAGAC CTGCCTCTCC CACCACCTCC TGCTAGCCAG
    ACAGGACCTG CCTCTGCTCA GGTGGTGGCT GCAGAGAGGA AGAAGAGGGA GGAGCACCAG
    CGCTGGTATG AGAAGGAGAA GGCCCGTCTA GAGGAGGAGC GGGACAGGAA GCGCCGTGAG
    CAGGAGCGAA AGCTGGGCCA GATGCGCTCC CAGTCACTGA ACCCAGCCCC GTTTTCTCCT
    GTAACTGTCC CCCATGTGAA GCCAGAAAAA CCTTCTACAC TCCAGAGGCC CCAAGAGACG
    GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG
    TACATTACAG TCAGCAAAGA GGAGCTGTCC TCAGGAGATA GTCTGTCCCC AGACCCGTGG
    AAGCGGGATG CCCGGGAGAA GCTAGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG
    AGCAGGGAGA TCCAGGAGCT CCAGAATAAG CCCGAGCGCA GTGCAGAGGA AAACGATCGC
    CTGCGGAAAC TCATGCTGGA GTGGCAGTTC CAGAAGAGGC TCCAGGAGTC CAAGCAGAAG
    GACGAGGATG ACGAGGAGGA GGAGGATGAT GACGTGGATA CCATGCTCAT CATGCAGCGC
    CTCGAGGCCG AGCGAAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGAGAAGCA
    GAAGATCGCG CCAGGCAGGA GGACGAGCGG CGCCGCCAGG AGGAGGAGAG AGCAAAGCGA
    GATGCTGAAG AAAAGAGGCG TCAGGAAGAA GGCTATTACA GCCGCCTGGA AGCCGAGAGG
    CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC
    AGACCTCCGC TGCCGCGGGG CTACAAGCTC CCGTCCCCGT CCCCTCCGGC TGCCGCTCCT
    CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCCC AGGCCCTCTC CCCAGACTCC
    CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGCA CTGCAGTCTG
    GCAGGTCAGG ACAAGCACTC TGGCACGAGG AAGTCCCACG GGCACCTTCT TCCCGCCGCC
    CTTGAGCCGC AGCGGCCCCC CCGCCCGCCG CGCCCGGCCT CCGACGGCAT CTTTCTCTGC
    ACCTCATTCC GGCCCCCCTT GGCCCGAGCC AACAGTACTG CTCACAAAGC AGGCCAGCCC
    CCACCCCCTC CAAAAAAACC AGGCTTCTAC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT
    TACCTGGGAT CTGCCGGGAC AGCTGTTGGC ACCCACGATG CCTATCGGGA CCCGAGAGAA
    AGACTGTCTA AAAGCCACGA GGCAGATTTA CCTGGGAGCT CTGGAGCCCC TGAAAACTTG
    ACATTCAAAG AGCGCCAGCG TCTGTTCTCA CAAGGTCAGG ACGTGTCCAA TAAAGTAAAA
    GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34490
    Clone ID Related Accession (Same CDS sequence) XM_014113184.1
    Accession Version XM_014113184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5625bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC TATTACAGCC GCCTGGAAGC CGAGAGGCGC
    AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA
    CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT
    CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCCCAGG CCCTCTCCCC AGACTCCCTG
    TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGCACTG CAGTCTGGCA
    GGTCAGGACA AGCACTCTGG CACGAGGAAG TCCCACGGGC ACCTTCTTCC CGCCGCCCTT
    GAGCCGCAGC GGCCCCCCCG CCCGCCGCGC CCGGCCTCCG ACGGCATCTT TCTCTGCACC
    TCATTCCGGC CCCCCTTGGC CCGAGCCAAC AGTACTGCTC ACAAAGCAGG CCAGCCCCCA
    CCCCCTCCAA AAAAACCAGG CTTCTACCAG CCCAGCCACT CGAAAGGACC AAACTCTTAC
    CTGGGATCTG CCGGGACAGC TGTTGGCACC CACGATGCCT ATCGGGACCC GAGAGAAAGA
    CTGTCTAAAA GCCACGAGGC AGATTTACCT GGGAGCTCTG GAGCCCCTGA AAACTTGACA
    TTCAAAGAGC GCCAGCGTCT GTTCTCACAA GGTCAGGACG TGTCCAATAA AGTAAAAGCC
    TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968659.1
    CDS28..5652
    Translation

    Target ORF information:

    RefSeq Version XM_014113184.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG AGAAGCAGAA
    GATCGCGCCA GGCAGGAGGA CGAGCGGCGC CGCCAGGAGG AGGAGAGAGC AAAGCGAGAT
    GCTGAAGAAA AGAGGCGTCA GGAAGAAGGC TATTACAGCC GCCTGGAAGC CGAGAGGCGC
    AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA
    CCTCCGCTGC CGCGGGGCTA CAAGCTCCCG TCCCCGTCCC CTCCGGCTGC CGCTCCTCCT
    CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCCCAGG CCCTCTCCCC AGACTCCCTG
    TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGCACTG CAGTCTGGCA
    GGTCAGGACA AGCACTCTGG CACGAGGAAG TCCCACGGGC ACCTTCTTCC CGCCGCCCTT
    GAGCCGCAGC GGCCCCCCCG CCCGCCGCGC CCGGCCTCCG ACGGCATCTT TCTCTGCACC
    TCATTCCGGC CCCCCTTGGC CCGAGCCAAC AGTACTGCTC ACAAAGCAGG CCAGCCCCCA
    CCCCCTCCAA AAAAACCAGG CTTCTACCAG CCCAGCCACT CGAAAGGACC AAACTCTTAC
    CTGGGATCTG CCGGGACAGC TGTTGGCACC CACGATGCCT ATCGGGACCC GAGAGAAAGA
    CTGTCTAAAA GCCACGAGGC AGATTTACCT GGGAGCTCTG GAGCCCCTGA AAACTTGACA
    TTCAAAGAGC GCCAGCGTCT GTTCTCACAA GGTCAGGACG TGTCCAATAA AGTAAAAGCC
    TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34491
    Clone ID Related Accession (Same CDS sequence) XM_014113187.1
    Accession Version XM_014113187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    GATGAGGAGC GGAGGCGCCA GCAGCAGCTG GAAGAGATGC GCAAGCGAGA AGCAGAAGAT
    CGCGCCAGGC AGGAGGACGA GCGGCGCCGC CAGGAGGAGG AGAGAGCAAA GCGAGATGCT
    GAAGAAAAGA GGCGTCAGGA AGAAGGCTAT TACAGCCGCC TGGAAGCCGA GAGGCGCAGA
    CAGCACGCGG AGGCCGAGCG CAGGTTGCTG GAGCCGGAGG AGCCGGGTCT GTGCAGACCT
    CCGCTGCCGC GGGGCTACAA GCTCCCGTCC CCGTCCCCTC CGGCTGCCGC TCCTCCTCCC
    CCGCCTCAGC GGACCGCCTC CTCCCTCCAG GCCCAGGCCC TCTCCCCAGA CTCCCTGTAC
    ACCGCCAGGC TCGTTGCGTA CCACGAGGAG GAGGAGGAGG AGCACTGCAG TCTGGCAGGT
    CAGGACAAGC ACTCTGGCAC GAGGAAGTCC CACGGGCACC TTCTTCCCGC CGCCCTTGAG
    CCGCAGCGGC CCCCCCGCCC GCCGCGCCCG GCCTCCGACG GCATCTTTCT CTGCACCTCA
    TTCCGGCCCC CCTTGGCCCG AGCCAACAGT ACTGCTCACA AAGCAGGCCA GCCCCCACCC
    CCTCCAAAAA AACCAGGCTT CTACCAGCCC AGCCACTCGA AAGGACCAAA CTCTTACCTG
    GGATCTGCCG GGACAGCTGT TGGCACCCAC GATGCCTATC GGGACCCGAG AGAAAGACTG
    TCTAAAAGCC ACGAGGCAGA TTTACCTGGG AGCTCTGGAG CCCCTGAAAA CTTGACATTC
    AAAGAGCGCC AGCGTCTGTT CTCACAAGGT CAGGACGTGT CCAATAAAGT AAAAGCCTCT
    CGTAAATTAA CAGAACTGGA AAACGAACTG AACACCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968662.1
    CDS28..5649
    Translation

    Target ORF information:

    RefSeq Version XM_014113187.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG
    GATGAGGAGC GGAGGCGCCA GCAGCAGCTG GAAGAGATGC GCAAGCGAGA AGCAGAAGAT
    CGCGCCAGGC AGGAGGACGA GCGGCGCCGC CAGGAGGAGG AGAGAGCAAA GCGAGATGCT
    GAAGAAAAGA GGCGTCAGGA AGAAGGCTAT TACAGCCGCC TGGAAGCCGA GAGGCGCAGA
    CAGCACGCGG AGGCCGAGCG CAGGTTGCTG GAGCCGGAGG AGCCGGGTCT GTGCAGACCT
    CCGCTGCCGC GGGGCTACAA GCTCCCGTCC CCGTCCCCTC CGGCTGCCGC TCCTCCTCCC
    CCGCCTCAGC GGACCGCCTC CTCCCTCCAG GCCCAGGCCC TCTCCCCAGA CTCCCTGTAC
    ACCGCCAGGC TCGTTGCGTA CCACGAGGAG GAGGAGGAGG AGCACTGCAG TCTGGCAGGT
    CAGGACAAGC ACTCTGGCAC GAGGAAGTCC CACGGGCACC TTCTTCCCGC CGCCCTTGAG
    CCGCAGCGGC CCCCCCGCCC GCCGCGCCCG GCCTCCGACG GCATCTTTCT CTGCACCTCA
    TTCCGGCCCC CCTTGGCCCG AGCCAACAGT ACTGCTCACA AAGCAGGCCA GCCCCCACCC
    CCTCCAAAAA AACCAGGCTT CTACCAGCCC AGCCACTCGA AAGGACCAAA CTCTTACCTG
    GGATCTGCCG GGACAGCTGT TGGCACCCAC GATGCCTATC GGGACCCGAG AGAAAGACTG
    TCTAAAAGCC ACGAGGCAGA TTTACCTGGG AGCTCTGGAG CCCCTGAAAA CTTGACATTC
    AAAGAGCGCC AGCGTCTGTT CTCACAAGGT CAGGACGTGT CCAATAAAGT AAAAGCCTCT
    CGTAAATTAA CAGAACTGGA AAACGAACTG AACACCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34492
    Clone ID Related Accession (Same CDS sequence) XM_014113190.1 , XM_022419031.1
    Accession Version XM_014113190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968665.1
    CDS28..5433
    Translation

    Target ORF information:

    RefSeq Version XM_014113190.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34492
    Clone ID Related Accession (Same CDS sequence) XM_014113190.1 , XM_022419031.1
    Accession Version XM_022419031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274739.1
    CDS43..5448
    Translation

    Target ORF information:

    RefSeq Version XM_022419031.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAGAA
    AGAAGATTGG ATATAGATGA GAAACCCCTA GTTGTGCAGT TGAATTGGAA TAAAGATGAT
    CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTGCTCCTAA GAAGGCTCAG
    AGCAATGGAC CCGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC
    TCCAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTGAG GCAGGCATCT
    GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACATTGAGA ATTCCCGACT GGCTGCTGAG
    GTCTACAAAG ACATGCCTGA GACCAGCTTT ACCCGAACAA TTTCGAACCC AGAGGTGGTG
    ATGAAGCGTC GCAGGCAGCA GAAACTGGAG AGGAGGATGC AGGAATTCCG CAGCTCGGAT
    GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCCG ATAGTTTAAA ACCAAATATT
    CCCTACAAGA CAATCCTGCT GTCTACCACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCG
    TTAGAGAAAT ATGGTCTAGA GAAAGAAAAT CCTAAGGATT ACTGCATTGC CCGAGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATGAT CCTGGATGAT
    GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGCG ACAAAGGGCT TCTAGTCTTT
    CAGTTGAAGA GGAGGCCAGC CGACTATATT CCAAAGAAAA CTAAGAAACA CGTCGATGGG
    AAGCTGGTGA AGGGAAAGGA GAGAGCAGAG GGGTCAGCCT ACGGTTCTGC ACTCCCTCCC
    GAGAAACTGC CCTATCTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AACTATCACA CTTATGAAGA TGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT
    CAGTTGAGCG TTACTGAGGT TGGGACGGAA AAGTTTGATG AGAACTCCAT CCAGCTGTTC
    GGATCAGGAA TTCAGCCCCA CCACTGCGAC CTCACAAACA TGGATGGAGT TGTCACTGTA
    ACGCCGAGAA GCATGGATGC TGAGACTTAC GTGGAAGGAC AGCGCATCTC GGAGACAACC
    ATGCTACAGA GCGGGATGAA AGTGCAGTTT GGGGCGTCAC ACGTGTTCAA GTTTGTGGAT
    CCCAGCCAGG ACCACGCTCT GGCAAAGCGA TCTGTGGATG GAAGCCTGAC CGCCAAGGGT
    CCCAGACATA AACCTGGAAT TGTTCAAGAG ACAACATTTG ATTTAGGAGG AGATGTTCAC
    AGTGGAACAG CATTACCAAC AAGTAAGAGC ACCACTCGGC TGGACGGCGA CAGGGTATTG
    TCTGCCTCCA GCACAGCGGA GCGAGGGATG GTGAAGCCGA TGATGAGAGT AGAGCAGCAG
    CAGGACTACC GCAGGCAAGA AAACAGAACT CAGGATGCTC CTGGGCCTGA GCTGATACTG
    CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAC
    TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA
    TGTCGGTATG TATTGTCAAG CCAGTACAGA CCCGACGTCA GCCCTACAGA GCGCACTCAC
    AAAGTGATTG CAATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAATATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAT
    GCATCTGAGC TGCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACACTGGAC
    GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCGTTTA AATATCTGGT TCATTGTCTT
    CAATCAGAAC TGAATAATTA CATGCCAGCC TTCCTGGATG ACCCCGAGGA GAACAGCCTA
    CAGAGACCAA AAATAGATGA TGTTTTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTTATC
    AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCTGAGT CGGGACTGTG CTCCCACTAC
    TGGGGGGCGA TTATTCGCCA GCAGCTAGGA CACGTGGAAG CTTGGGCAGA GAAGCAGGGG
    CTGGAGCTGG CAGCGGACTG TCACCTCAGC AGGATCGTGC AGGCAACCAC TTTGCTTACA
    ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAT
    TCACTGCAAC TTCAAGCCTT GTTACAGAAT TACCACTGTG CACCTGATGA GCCTTTTATT
    CCCACGGACC TGATAGAGAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA CGAGCTGGCG
    CGCAGTGATG GCCGGGAGGT GCAGCTGGAA GAGGACCCCG ATCTGCAGTT GCCTTTTCTT
    TTGCCAGAAG ACGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTTTGTGCCA GAGAGGATTT TGCAGGTTAA TCCCTCATGC ACGTTCACCA
    GGTACTTGGA CAATATATTT TGAGGGTGCA GATTACGAGA GCCATCTTCT GCGTGAGAAC
    ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAGAAG
    CAAAATGGGA TGGGCCTTAG CATCGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA
    ATCTATGTCA AGTCTGTTGT AAAAGGCGGT GCTGCGGATG TGGATGGACG CCTGGCTGCG
    GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG
    GCAGAGCTCA TGACAAGAAC AAGTTCTGTG GTGACACTAG AAGTAGCAAA GCAAGGAGCA
    ATTTATCACG GCCTCGCCAC GCTGCTTAAT CAGCCATCCC CAATGATGCA AAGAATTTCA
    GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT
    TCAGCTCAAA ACGGGTCACC TGAGAGCCCT CAGCTGCCTT GGGCAGAATA CACTGAACCC
    AAGAAATTGC CTGGCGATGA CAGACTGATG AAGAACCGGG CTGATCACCG CTCCAGCCCC
    AACGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAAAGCG CATACGCCCC TGGAACAACA
    GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT
    AGACCTGAGG CTTACCCCAT CCCCACTCAG ACATACACCA GAGAGTATTT CACCTTCCCA
    GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG
    AAGCCACACA TGCACATGGA CAATAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT
    TCCCAGGAGG AGCTTCGGGA GGATAAGGCT TACCAACTAG AGCGGCATCG GATCGAGGCA
    GTCCTGGATC GCAAGTCTGA TAGCGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCG
    AGCACTTCCA GCCAAGAGCA CCTGGACCAC CCCTCCAAGG CCGGCACCCC CGCTTCCACT
    CTGACCAAGA GTGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CCTGCCTGTG
    GCTGGCTCCC AGCCGGTCCA AACTGACCTG CCCCCACCAC CCCCGCCGCC CCCCACGCAC
    TACGCCGGAG ACTTCGACGG GATCCCAACA GACCTGCCTC TCCCACCACC TCCTGCTAGC
    CAGACAGGAC CTGCCTCTGC TCAGGTGGTG GCTGCAGAGA GGAAGAAGAG GGAGGAGCAC
    CAGCGCTGGT ATGAGAAGGA GAAGGCCCGT CTAGAGGAGG AGCGGGACAG GAAGCGCCGT
    GAGCAGGAGC GAAAGCTGGG CCAGATGCGC TCCCAGTCAC TGAACCCAGC CCCGTTTTCT
    CCTGTAACTG TCCCCCATGT GAAGCCAGAA AAACCTTCTA CACTCCAGAG GCCCCAAGAG
    ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA
    CAGTACATTA CAGTCAGCAA AGAGGAGCTG TCCTCAGGAG ATAGTCTGTC CCCAGACCCG
    TGGAAGCGGG ATGCCCGGGA GAAGCTAGAG AAGCAGCAGC AGATGCACAT CGTGGATATG
    CTGAGCAGGG AGATCCAGGA GCTCCAGAAT AAGCCCGAGC GCAGTGCAGA GGAAAACGAT
    CGCCTGCGGA AACTCATGCT GGAGTGGCAG TTCCAGAAGA GGCTCCAGGA GTCCAAGCAG
    AAGGACGAGG ATGACGAGGA GGAGGAGGAT GATGACGTGG ATACCATGCT CATCATGCAG
    CGCCTCGAGG CCGAGCGAAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT
    TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34493
    Clone ID Related Accession (Same CDS sequence) XM_014113194.1
    Accession Version XM_014113194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GAAACAGAAG
    AATATTGCAG GGGCCCTTGC CTTCTGGATG GCCAATGCAT CTGAGCTGCT CAACTTCATT
    AAGCAGGACC GAGATCTTAG TCGAATCACA CTGGACGCCC AGGACGTTTT AGCACACTTG
    GTTCAGATGG CGTTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG
    CCAGCCTTCC TGGATGACCC CGAGGAGAAC AGCCTACAGA GACCAAAAAT AGATGATGTT
    TTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCTGCCCTG
    ACCATCCAGC TCTTCTCTCA GCTCTTCCAC TTTATCAACA TGTGGCTCTT CAACAGATTG
    GTGACCGACC CTGAGTCGGG ACTGTGCTCC CACTACTGGG GGGCGATTAT TCGCCAGCAG
    CTAGGACACG TGGAAGCTTG GGCAGAGAAG CAGGGGCTGG AGCTGGCAGC GGACTGTCAC
    CTCAGCAGGA TCGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC
    ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAATTCAC TGCAACTTCA AGCCTTGTTA
    CAGAATTACC ACTGTGCACC TGATGAGCCT TTTATTCCCA CGGACCTGAT AGAGAATGTG
    GTGGCTGTGG CTGAAAACAC GGCTGACGAG CTGGCGCGCA GTGATGGCCG GGAGGTGCAG
    CTGGAAGAGG ACCCCGATCT GCAGTTGCCT TTTCTTTTGC CAGAAGACGG CTATTCTTGT
    GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TAGACCCTTT GTGCCAGAGA
    GGATTTTGCA GGTTAATCCC TCATGCACGT TCACCAGGTA CTTGGACAAT ATATTTTGAG
    GGTGCAGATT ACGAGAGCCA TCTTCTGCGT GAGAACACGG AGCTGGCTCA GCCTCTGAGG
    AAAGAACCTG AAATAATCAC TGTGACTCTT AAGAAGCAAA ATGGGATGGG CCTTAGCATC
    GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTTGGAATCT ATGTCAAGTC TGTTGTAAAA
    GGCGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCGGGTG ACCAGCTCCT CAGTGTGGAC
    GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AGCTCATGAC AAGAACAAGT
    TCTGTGGTGA CACTAGAAGT AGCAAAGCAA GGAGCAATTT ATCACGGCCT CGCCACGCTG
    CTTAATCAGC CATCCCCAAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC
    CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCAAAACGG GTCACCTGAG
    AGCCCTCAGC TGCCTTGGGC AGAATACACT GAACCCAAGA AATTGCCTGG CGATGACAGA
    CTGATGAAGA ACCGGGCTGA TCACCGCTCC AGCCCCAACG TGGCAAATCA GCCTCCTAGC
    CCTGGAGGGA AAAGCGCATA CGCCCCTGGA ACAACAGCTA AGATAACGTC TGTCTCCACT
    GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCTTA CCCCATCCCC
    ACTCAGACAT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG
    GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CATGGACAAT
    AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AGGAGGAGCT TCGGGAGGAT
    AAGGCTTACC AACTAGAGCG GCATCGGATC GAGGCAGTCC TGGATCGCAA GTCTGATAGC
    GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCGAGCA CTTCCAGCCA AGAGCACCTG
    GACCACCCCT CCAAGGCCGG CACCCCCGCT TCCACTCTGA CCAAGAGTGG CCCTGGGCGC
    TGGAAGACGC CAGCCGCTGT GCAGCCCCTG CCTGTGGCTG GCTCCCAGCC GGTCCAAACT
    GACCTGCCCC CACCACCCCC GCCGCCCCCC ACGCACTACG CCGGAGACTT CGACGGGATC
    CCAACAGACC TGCCTCTCCC ACCACCTCCT GCTAGCCAGA CAGGACCTGC CTCTGCTCAG
    GTGGTGGCTG CAGAGAGGAA GAAGAGGGAG GAGCACCAGC GCTGGTATGA GAAGGAGAAG
    GCCCGTCTAG AGGAGGAGCG GGACAGGAAG CGCCGTGAGC AGGAGCGAAA GCTGGGCCAG
    ATGCGCTCCC AGTCACTGAA CCCAGCCCCG TTTTCTCCTG TAACTGTCCC CCATGTGAAG
    CCAGAAAAAC CTTCTACACT CCAGAGGCCC CAAGAGACGG TCATTCGGGA GCTGCAGCCC
    CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACAGT CAGCAAAGAG
    GAGCTGTCCT CAGGAGATAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CCGGGAGAAG
    CTAGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCAGGAGCTC
    CAGAATAAGC CCGAGCGCAG TGCAGAGGAA AACGATCGCC TGCGGAAACT CATGCTGGAG
    TGGCAGTTCC AGAAGAGGCT CCAGGAGTCC AAGCAGAAGG ACGAGGATGA CGAGGAGGAG
    GAGGATGATG ACGTGGATAC CATGCTCATC ATGCAGCGCC TCGAGGCCGA GCGAAGAGCA
    AGGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGTC AGGACAAGCA CTCTGGCACG AGGAAGTCCC ACGGGCACCT TCTTCCCGCC
    GCCCTTGAGC CGCAGCGGCC CCCCCGCCCG CCGCGCCCGG CCTCCGACGG CATCTTTCTC
    TGCACCTCAT TCCGGCCCCC CTTGGCCCGA GCCAACAGTA CTGCTCACAA AGCAGGCCAG
    CCCCCACCCC CTCCAAAAAA ACCAGGCTTC TACCAGCCCA GCCACTCGAA AGGACCAAAC
    TCTTACCTGG GATCTGCCGG GACAGCTGTT GGCACCCACG ATGCCTATCG GGACCCGAGA
    GAAAGACTGT CTAAAAGCCA CGAGGCAGAT TTACCTGGGA GCTCTGGAGC CCCTGAAAAC
    TTGACATTCA AAGAGCGCCA GCGTCTGTTC TCACAAGGTC AGGACGTGTC CAATAAAGTA
    AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968669.1
    CDS22..5592
    Translation

    Target ORF information:

    RefSeq Version XM_014113194.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GAAACAGAAG
    AATATTGCAG GGGCCCTTGC CTTCTGGATG GCCAATGCAT CTGAGCTGCT CAACTTCATT
    AAGCAGGACC GAGATCTTAG TCGAATCACA CTGGACGCCC AGGACGTTTT AGCACACTTG
    GTTCAGATGG CGTTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG
    CCAGCCTTCC TGGATGACCC CGAGGAGAAC AGCCTACAGA GACCAAAAAT AGATGATGTT
    TTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCTGCCCTG
    ACCATCCAGC TCTTCTCTCA GCTCTTCCAC TTTATCAACA TGTGGCTCTT CAACAGATTG
    GTGACCGACC CTGAGTCGGG ACTGTGCTCC CACTACTGGG GGGCGATTAT TCGCCAGCAG
    CTAGGACACG TGGAAGCTTG GGCAGAGAAG CAGGGGCTGG AGCTGGCAGC GGACTGTCAC
    CTCAGCAGGA TCGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC
    ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAATTCAC TGCAACTTCA AGCCTTGTTA
    CAGAATTACC ACTGTGCACC TGATGAGCCT TTTATTCCCA CGGACCTGAT AGAGAATGTG
    GTGGCTGTGG CTGAAAACAC GGCTGACGAG CTGGCGCGCA GTGATGGCCG GGAGGTGCAG
    CTGGAAGAGG ACCCCGATCT GCAGTTGCCT TTTCTTTTGC CAGAAGACGG CTATTCTTGT
    GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TAGACCCTTT GTGCCAGAGA
    GGATTTTGCA GGTTAATCCC TCATGCACGT TCACCAGGTA CTTGGACAAT ATATTTTGAG
    GGTGCAGATT ACGAGAGCCA TCTTCTGCGT GAGAACACGG AGCTGGCTCA GCCTCTGAGG
    AAAGAACCTG AAATAATCAC TGTGACTCTT AAGAAGCAAA ATGGGATGGG CCTTAGCATC
    GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTTGGAATCT ATGTCAAGTC TGTTGTAAAA
    GGCGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCGGGTG ACCAGCTCCT CAGTGTGGAC
    GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AGCTCATGAC AAGAACAAGT
    TCTGTGGTGA CACTAGAAGT AGCAAAGCAA GGAGCAATTT ATCACGGCCT CGCCACGCTG
    CTTAATCAGC CATCCCCAAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC
    CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCAAAACGG GTCACCTGAG
    AGCCCTCAGC TGCCTTGGGC AGAATACACT GAACCCAAGA AATTGCCTGG CGATGACAGA
    CTGATGAAGA ACCGGGCTGA TCACCGCTCC AGCCCCAACG TGGCAAATCA GCCTCCTAGC
    CCTGGAGGGA AAAGCGCATA CGCCCCTGGA ACAACAGCTA AGATAACGTC TGTCTCCACT
    GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCTTA CCCCATCCCC
    ACTCAGACAT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG
    GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CATGGACAAT
    AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AGGAGGAGCT TCGGGAGGAT
    AAGGCTTACC AACTAGAGCG GCATCGGATC GAGGCAGTCC TGGATCGCAA GTCTGATAGC
    GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCGAGCA CTTCCAGCCA AGAGCACCTG
    GACCACCCCT CCAAGGCCGG CACCCCCGCT TCCACTCTGA CCAAGAGTGG CCCTGGGCGC
    TGGAAGACGC CAGCCGCTGT GCAGCCCCTG CCTGTGGCTG GCTCCCAGCC GGTCCAAACT
    GACCTGCCCC CACCACCCCC GCCGCCCCCC ACGCACTACG CCGGAGACTT CGACGGGATC
    CCAACAGACC TGCCTCTCCC ACCACCTCCT GCTAGCCAGA CAGGACCTGC CTCTGCTCAG
    GTGGTGGCTG CAGAGAGGAA GAAGAGGGAG GAGCACCAGC GCTGGTATGA GAAGGAGAAG
    GCCCGTCTAG AGGAGGAGCG GGACAGGAAG CGCCGTGAGC AGGAGCGAAA GCTGGGCCAG
    ATGCGCTCCC AGTCACTGAA CCCAGCCCCG TTTTCTCCTG TAACTGTCCC CCATGTGAAG
    CCAGAAAAAC CTTCTACACT CCAGAGGCCC CAAGAGACGG TCATTCGGGA GCTGCAGCCC
    CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACAGT CAGCAAAGAG
    GAGCTGTCCT CAGGAGATAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CCGGGAGAAG
    CTAGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCAGGAGCTC
    CAGAATAAGC CCGAGCGCAG TGCAGAGGAA AACGATCGCC TGCGGAAACT CATGCTGGAG
    TGGCAGTTCC AGAAGAGGCT CCAGGAGTCC AAGCAGAAGG ACGAGGATGA CGAGGAGGAG
    GAGGATGATG ACGTGGATAC CATGCTCATC ATGCAGCGCC TCGAGGCCGA GCGAAGAGCA
    AGGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGTC AGGACAAGCA CTCTGGCACG AGGAAGTCCC ACGGGCACCT TCTTCCCGCC
    GCCCTTGAGC CGCAGCGGCC CCCCCGCCCG CCGCGCCCGG CCTCCGACGG CATCTTTCTC
    TGCACCTCAT TCCGGCCCCC CTTGGCCCGA GCCAACAGTA CTGCTCACAA AGCAGGCCAG
    CCCCCACCCC CTCCAAAAAA ACCAGGCTTC TACCAGCCCA GCCACTCGAA AGGACCAAAC
    TCTTACCTGG GATCTGCCGG GACAGCTGTT GGCACCCACG ATGCCTATCG GGACCCGAGA
    GAAAGACTGT CTAAAAGCCA CGAGGCAGAT TTACCTGGGA GCTCTGGAGC CCCTGAAAAC
    TTGACATTCA AAGAGCGCCA GCGTCTGTTC TCACAAGGTC AGGACGTGTC CAATAAAGTA
    AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34494
    Clone ID Related Accession (Same CDS sequence) XM_014113196.1
    Accession Version XM_014113196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGCAGAC TGCTCTGCCA GCAATCTCTG TTCTAGATTT GTTACAGGAT
    GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA GAGATGCGCA AGCGAGAAGC AGAAGATCGC
    GCCAGGCAGG AGGACGAGCG GCGCCGCCAG GAGGAGGAGA GAGCAAAGCG AGATGCTGAA
    GAAAAGAGGC GTCAGGAAGA AGGCTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGCGGAGG CCGAGCGCAG GTTGCTGGAG CCGGAGGAGC CGGGTCTGTG CAGACCTCCG
    CTGCCGCGGG GCTACAAGCT CCCGTCCCCG TCCCCTCCGG CTGCCGCTCC TCCTCCCCCG
    CCTCAGCGGA CCGCCTCCTC CCTCCAGGCC CAGGCCCTCT CCCCAGACTC CCTGTACACC
    GCCAGGCTCG TTGCGTACCA CGAGGAGGAG GAGGAGGAGC ACTGCAGTCT GGCAGGTCAG
    GACAAGCACT CTGGCACGAG GAAGTCCCAC GGGCACCTTC TTCCCGCCGC CCTTGAGCCG
    CAGCGGCCCC CCCGCCCGCC GCGCCCGGCC TCCGACGGCA TCTTTCTCTG CACCTCATTC
    CGGCCCCCCT TGGCCCGAGC CAACAGTACT GCTCACAAAG CAGGCCAGCC CCCACCCCCT
    CCAAAAAAAC CAGGCTTCTA CCAGCCCAGC CACTCGAAAG GACCAAACTC TTACCTGGGA
    TCTGCCGGGA CAGCTGTTGG CACCCACGAT GCCTATCGGG ACCCGAGAGA AAGACTGTCT
    AAAAGCCACG AGGCAGATTT ACCTGGGAGC TCTGGAGCCC CTGAAAACTT GACATTCAAA
    GAGCGCCAGC GTCTGTTCTC ACAAGGTCAG GACGTGTCCA ATAAAGTAAA AGCCTCTCGT
    AAATTAACAG AACTGGAAAA CGAACTGAAC ACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968671.1
    CDS22..5580
    Translation

    Target ORF information:

    RefSeq Version XM_014113196.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGCAGAC TGCTCTGCCA GCAATCTCTG TTCTAGATTT GTTACAGGAT
    GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA GAGATGCGCA AGCGAGAAGC AGAAGATCGC
    GCCAGGCAGG AGGACGAGCG GCGCCGCCAG GAGGAGGAGA GAGCAAAGCG AGATGCTGAA
    GAAAAGAGGC GTCAGGAAGA AGGCTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG
    CACGCGGAGG CCGAGCGCAG GTTGCTGGAG CCGGAGGAGC CGGGTCTGTG CAGACCTCCG
    CTGCCGCGGG GCTACAAGCT CCCGTCCCCG TCCCCTCCGG CTGCCGCTCC TCCTCCCCCG
    CCTCAGCGGA CCGCCTCCTC CCTCCAGGCC CAGGCCCTCT CCCCAGACTC CCTGTACACC
    GCCAGGCTCG TTGCGTACCA CGAGGAGGAG GAGGAGGAGC ACTGCAGTCT GGCAGGTCAG
    GACAAGCACT CTGGCACGAG GAAGTCCCAC GGGCACCTTC TTCCCGCCGC CCTTGAGCCG
    CAGCGGCCCC CCCGCCCGCC GCGCCCGGCC TCCGACGGCA TCTTTCTCTG CACCTCATTC
    CGGCCCCCCT TGGCCCGAGC CAACAGTACT GCTCACAAAG CAGGCCAGCC CCCACCCCCT
    CCAAAAAAAC CAGGCTTCTA CCAGCCCAGC CACTCGAAAG GACCAAACTC TTACCTGGGA
    TCTGCCGGGA CAGCTGTTGG CACCCACGAT GCCTATCGGG ACCCGAGAGA AAGACTGTCT
    AAAAGCCACG AGGCAGATTT ACCTGGGAGC TCTGGAGCCC CTGAAAACTT GACATTCAAA
    GAGCGCCAGC GTCTGTTCTC ACAAGGTCAG GACGTGTCCA ATAAAGTAAA AGCCTCTCGT
    AAATTAACAG AACTGGAAAA CGAACTGAAC ACCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34495
    Clone ID Related Accession (Same CDS sequence) XM_014113198.1
    Accession Version XM_014113198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product afadin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GAAACAGAAG
    AATATTGCAG GGGCCCTTGC CTTCTGGATG GCCAATGCAT CTGAGCTGCT CAACTTCATT
    AAGCAGGACC GAGATCTTAG TCGAATCACA CTGGACGCCC AGGACGTTTT AGCACACTTG
    GTTCAGATGG CGTTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG
    CCAGCCTTCC TGGATGACCC CGAGGAGAAC AGCCTACAGA GACCAAAAAT AGATGATGTT
    TTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCTGCCCTG
    ACCATCCAGC TCTTCTCTCA GCTCTTCCAC TTTATCAACA TGTGGCTCTT CAACAGATTG
    GTGACCGACC CTGAGTCGGG ACTGTGCTCC CACTACTGGG GGGCGATTAT TCGCCAGCAG
    CTAGGACACG TGGAAGCTTG GGCAGAGAAG CAGGGGCTGG AGCTGGCAGC GGACTGTCAC
    CTCAGCAGGA TCGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC
    ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAATTCAC TGCAACTTCA AGCCTTGTTA
    CAGAATTACC ACTGTGCACC TGATGAGCCT TTTATTCCCA CGGACCTGAT AGAGAATGTG
    GTGGCTGTGG CTGAAAACAC GGCTGACGAG CTGGCGCGCA GTGATGGCCG GGAGGTGCAG
    CTGGAAGAGG ACCCCGATCT GCAGTTGCCT TTTCTTTTGC CAGAAGACGG CTATTCTTGT
    GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TAGACCCTTT GTGCCAGAGA
    GGATTTTGCA GGTTAATCCC TCATGCACGT TCACCAGGTA CTTGGACAAT ATATTTTGAG
    GGTGCAGATT ACGAGAGCCA TCTTCTGCGT GAGAACACGG AGCTGGCTCA GCCTCTGAGG
    AAAGAACCTG AAATAATCAC TGTGACTCTT AAGAAGCAAA ATGGGATGGG CCTTAGCATC
    GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTTGGAATCT ATGTCAAGTC TGTTGTAAAA
    GGCGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCGGGTG ACCAGCTCCT CAGTGTGGAC
    GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AGCTCATGAC AAGAACAAGT
    TCTGTGGTGA CACTAGAAGT AGCAAAGCAA GGAGCAATTT ATCACGGCCT CGCCACGCTG
    CTTAATCAGC CATCCCCAAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC
    CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCAAAACGG GTCACCTGAG
    AGCCCTCAGC TGCCTTGGGC AGAATACACT GAACCCAAGA AATTGCCTGG CGATGACAGA
    CTGATGAAGA ACCGGGCTGA TCACCGCTCC AGCCCCAACG TGGCAAATCA GCCTCCTAGC
    CCTGGAGGGA AAAGCGCATA CGCCCCTGGA ACAACAGCTA AGATAACGTC TGTCTCCACT
    GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCTTA CCCCATCCCC
    ACTCAGACAT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG
    GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CATGGACAAT
    AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AGGAGGAGCT TCGGGAGGAT
    AAGGCTTACC AACTAGAGCG GCATCGGATC GAGGCAGTCC TGGATCGCAA GTCTGATAGC
    GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCGAGCA CTTCCAGCCA AGAGCACCTG
    GACCACCCCT CCAAGGCCGG CACCCCCGCT TCCACTCTGA CCAAGAGTGG CCCTGGGCGC
    TGGAAGACGC CAGCCGCTGT GCAGCCCCTG CCTGTGGCTG GCTCCCAGCC GGTCCAAACT
    GACCTGCCCC CACCACCCCC GCCGCCCCCC ACGCACTACG CCGGAGACTT CGACGGGATC
    CCAACAGACC TGCCTCTCCC ACCACCTCCT GCTAGCCAGA CAGGACCTGC CTCTGCTCAG
    GTGGTGGCTG CAGAGAGGAA GAAGAGGGAG GAGCACCAGC GCTGGTATGA GAAGGAGAAG
    GCCCGTCTAG AGGAGGAGCG GGACAGGAAG CGCCGTGAGC AGGAGCGAAA GCTGGGCCAG
    ATGCGCTCCC AGTCACTGAA CCCAGCCCCG TTTTCTCCTG TAACTGTCCC CCATGTGAAG
    CCAGAAAAAC CTTCTACACT CCAGAGGCCC CAAGAGACGG TCATTCGGGA GCTGCAGCCC
    CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACAGT CAGCAAAGAG
    GAGCTGTCCT CAGGAGATAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CCGGGAGAAG
    CTAGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCAGGAGCTC
    CAGAATAAGC CCGAGCGCAG TGCAGAGGAA AACGATCGCC TGCGGAAACT CATGCTGGAG
    TGGCAGTTCC AGAAGAGGCT CCAGGAGTCC AAGCAGAAGG ACGAGGATGA CGAGGAGGAG
    GAGGATGATG ACGTGGATAC CATGCTCATC ATGCAGCGCC TCGAGGCCGA GCGAAGAGCA
    AGGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968673.1
    CDS22..5367
    Translation

    Target ORF information:

    RefSeq Version XM_014113198.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGAA GGCTCAGAGC
    AATGGACCCG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC
    AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTGAGGCA GGCATCTGAT
    AAGGATGATA GGCCTTTCCA AGGGGATGAC ATTGAGAATT CCCGACTGGC TGCTGAGGTC
    TACAAAGACA TGCCTGAGAC CAGCTTTACC CGAACAATTT CGAACCCAGA GGTGGTGATG
    AAGCGTCGCA GGCAGCAGAA ACTGGAGAGG AGGATGCAGG AATTCCGCAG CTCGGATGGG
    CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCCGATA GTTTAAAACC AAATATTCCC
    TACAAGACAA TCCTGCTGTC TACCACAGAT CCTGCAGACT TTGCTGTGGC TGAAGCGTTA
    GAGAAATATG GTCTAGAGAA AGAAAATCCT AAGGATTACT GCATTGCCCG AGTTATGCTT
    CCTCCTGGAG CCCAGCATTC TGATGAAAAA GGTGCTAAAG AAATGATCCT GGATGATGAT
    GAGTGTCCTT TACAAATCTT CAGGGAATGG CCAAGCGACA AAGGGCTTCT AGTCTTTCAG
    TTGAAGAGGA GGCCAGCCGA CTATATTCCA AAGAAAACTA AGAAACACGT CGATGGGAAG
    CTGGTGAAGG GAAAGGAGAG AGCAGAGGGG TCAGCCTACG GTTCTGCACT CCCTCCCGAG
    AAACTGCCCT ATCTAGTAGA GTTAAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC
    TATCACACTT ATGAAGATGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG
    TTGAGCGTTA CTGAGGTTGG GACGGAAAAG TTTGATGAGA ACTCCATCCA GCTGTTCGGA
    TCAGGAATTC AGCCCCACCA CTGCGACCTC ACAAACATGG ATGGAGTTGT CACTGTAACG
    CCGAGAAGCA TGGATGCTGA GACTTACGTG GAAGGACAGC GCATCTCGGA GACAACCATG
    CTACAGAGCG GGATGAAAGT GCAGTTTGGG GCGTCACACG TGTTCAAGTT TGTGGATCCC
    AGCCAGGACC ACGCTCTGGC AAAGCGATCT GTGGATGGAA GCCTGACCGC CAAGGGTCCC
    AGACATAAAC CTGGAATTGT TCAAGAGACA ACATTTGATT TAGGAGGAGA TGTTCACAGT
    GGAACAGCAT TACCAACAAG TAAGAGCACC ACTCGGCTGG ACGGCGACAG GGTATTGTCT
    GCCTCCAGCA CAGCGGAGCG AGGGATGGTG AAGCCGATGA TGAGAGTAGA GCAGCAGCAG
    GACTACCGCA GGCAAGAAAA CAGAACTCAG GATGCTCCTG GGCCTGAGCT GATACTGCCT
    GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAACTAT
    ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCACCTACAT ATGTATTATA TATGGCATGT
    CGGTATGTAT TGTCAAGCCA GTACAGACCC GACGTCAGCC CTACAGAGCG CACTCACAAA
    GTGATTGCAA TAGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GAAACAGAAG
    AATATTGCAG GGGCCCTTGC CTTCTGGATG GCCAATGCAT CTGAGCTGCT CAACTTCATT
    AAGCAGGACC GAGATCTTAG TCGAATCACA CTGGACGCCC AGGACGTTTT AGCACACTTG
    GTTCAGATGG CGTTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG
    CCAGCCTTCC TGGATGACCC CGAGGAGAAC AGCCTACAGA GACCAAAAAT AGATGATGTT
    TTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCTGCCCTG
    ACCATCCAGC TCTTCTCTCA GCTCTTCCAC TTTATCAACA TGTGGCTCTT CAACAGATTG
    GTGACCGACC CTGAGTCGGG ACTGTGCTCC CACTACTGGG GGGCGATTAT TCGCCAGCAG
    CTAGGACACG TGGAAGCTTG GGCAGAGAAG CAGGGGCTGG AGCTGGCAGC GGACTGTCAC
    CTCAGCAGGA TCGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC
    ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAATTCAC TGCAACTTCA AGCCTTGTTA
    CAGAATTACC ACTGTGCACC TGATGAGCCT TTTATTCCCA CGGACCTGAT AGAGAATGTG
    GTGGCTGTGG CTGAAAACAC GGCTGACGAG CTGGCGCGCA GTGATGGCCG GGAGGTGCAG
    CTGGAAGAGG ACCCCGATCT GCAGTTGCCT TTTCTTTTGC CAGAAGACGG CTATTCTTGT
    GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TAGACCCTTT GTGCCAGAGA
    GGATTTTGCA GGTTAATCCC TCATGCACGT TCACCAGGTA CTTGGACAAT ATATTTTGAG
    GGTGCAGATT ACGAGAGCCA TCTTCTGCGT GAGAACACGG AGCTGGCTCA GCCTCTGAGG
    AAAGAACCTG AAATAATCAC TGTGACTCTT AAGAAGCAAA ATGGGATGGG CCTTAGCATC
    GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTTGGAATCT ATGTCAAGTC TGTTGTAAAA
    GGCGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCGGGTG ACCAGCTCCT CAGTGTGGAC
    GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AGCTCATGAC AAGAACAAGT
    TCTGTGGTGA CACTAGAAGT AGCAAAGCAA GGAGCAATTT ATCACGGCCT CGCCACGCTG
    CTTAATCAGC CATCCCCAAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC
    CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCAAAACGG GTCACCTGAG
    AGCCCTCAGC TGCCTTGGGC AGAATACACT GAACCCAAGA AATTGCCTGG CGATGACAGA
    CTGATGAAGA ACCGGGCTGA TCACCGCTCC AGCCCCAACG TGGCAAATCA GCCTCCTAGC
    CCTGGAGGGA AAAGCGCATA CGCCCCTGGA ACAACAGCTA AGATAACGTC TGTCTCCACT
    GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCTTA CCCCATCCCC
    ACTCAGACAT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG
    GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CATGGACAAT
    AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AGGAGGAGCT TCGGGAGGAT
    AAGGCTTACC AACTAGAGCG GCATCGGATC GAGGCAGTCC TGGATCGCAA GTCTGATAGC
    GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCGAGCA CTTCCAGCCA AGAGCACCTG
    GACCACCCCT CCAAGGCCGG CACCCCCGCT TCCACTCTGA CCAAGAGTGG CCCTGGGCGC
    TGGAAGACGC CAGCCGCTGT GCAGCCCCTG CCTGTGGCTG GCTCCCAGCC GGTCCAAACT
    GACCTGCCCC CACCACCCCC GCCGCCCCCC ACGCACTACG CCGGAGACTT CGACGGGATC
    CCAACAGACC TGCCTCTCCC ACCACCTCCT GCTAGCCAGA CAGGACCTGC CTCTGCTCAG
    GTGGTGGCTG CAGAGAGGAA GAAGAGGGAG GAGCACCAGC GCTGGTATGA GAAGGAGAAG
    GCCCGTCTAG AGGAGGAGCG GGACAGGAAG CGCCGTGAGC AGGAGCGAAA GCTGGGCCAG
    ATGCGCTCCC AGTCACTGAA CCCAGCCCCG TTTTCTCCTG TAACTGTCCC CCATGTGAAG
    CCAGAAAAAC CTTCTACACT CCAGAGGCCC CAAGAGACGG TCATTCGGGA GCTGCAGCCC
    CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACAGT CAGCAAAGAG
    GAGCTGTCCT CAGGAGATAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CCGGGAGAAG
    CTAGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCAGGAGCTC
    CAGAATAAGC CCGAGCGCAG TGCAGAGGAA AACGATCGCC TGCGGAAACT CATGCTGGAG
    TGGCAGTTCC AGAAGAGGCT CCAGGAGTCC AAGCAGAAGG ACGAGGATGA CGAGGAGGAG
    GAGGATGATG ACGTGGATAC CATGCTCATC ATGCAGCGCC TCGAGGCCGA GCGAAGAGCA
    AGGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGAGAA
    GCAGAAGATC GCGCCAGGCA GGAGGACGAG CGGCGCCGCC AGGAGGAGGA GAGAGCAAAG
    CGAGATGCTG AAGAAAAGAG GCGTCAGGAA GAAGGCTATT ACAGCCGCCT GGAAGCCGAG
    AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG
    TGCAGACCTC CGCTGCCGCG GGGCTACAAG CTCCCGTCCC CGTCCCCTCC GGCTGCCGCT
    CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CCCAGGCCCT CTCCCCAGAC
    TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GCACTGCAGT
    CTGGCAGGAC CAAACTCTTA CCTGGGATCT GCCGGGACAG CTGTTGGCAC CCACGATGCC
    TATCGGGACC CGAGAGAAAG ACTGTCTAAA AGCCACGAGG CAGATTTACC TGGGAGCTCT
    GGAGCCCCTG AAAACTTGAC ATTCAAAGAG CGCCAGCGTC TGTTCTCACA AGGTCAGGAC
    GTGTCCAATA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACC
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34497
    Clone ID Related Accession (Same CDS sequence) XM_014113201.1
    Accession Version XM_014113201.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product afadin isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGTTACA GGATGAGGAG CGGAGGCGCC AGCAGCAGCT GGAAGAGATG
    CGCAAGCGAG AAGCAGAAGA TCGCGCCAGG CAGGAGGACG AGCGGCGCCG CCAGGAGGAG
    GAGAGAGCAA AGCGAGATGC TGAAGAAAAG AGGCGTCAGG AAGAAGGCTA TTACAGCCGC
    CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGTTGCT GGAGCCGGAG
    GAGCCGGGTC TGTGCAGACC TCCGCTGCCG CGGGGCTACA AGCTCCCGTC CCCGTCCCCT
    CCGGCTGCCG CTCCTCCTCC CCCGCCTCAG CGGACCGCCT CCTCCCTCCA GGCCCAGGCC
    CTCTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG
    GAGCACTGCA GTCTGGCAGG TCAGGACAAG CACTCTGGCA CGAGGAAGTC CCACGGGCAC
    CTTCTTCCCG CCGCCCTTGA GCCGCAGCGG CCCCCCCGCC CGCCGCGCCC GGCCTCCGAC
    GGCATCTTTC TCTGCACCTC ATTCCGGCCC CCCTTGGCCC GAGCCAACAG TACTGCTCAC
    AAAGCAGGCC AGCCCCCACC CCCTCCAAAA AAACCAGGCT TCTACCAGCC CAGCCACTCG
    AAAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968676.1
    CDS22..5544
    Translation

    Target ORF information:

    RefSeq Version XM_014113201.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113201.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
    TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CAGAGAAATT CCGGCCTGAC
    ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TGCATGTCAG TGGAGAAAGA
    AGATTGGATA TAGATGAGAA ACCCCTAGTT GTGCAGTTGA ATTGGAATAA AGATGATCGA
    GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTG CTCCTAAGGC TCAGAGCAAT
    GGACCCGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG
    AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TGAGGCAGGC ATCTGATAAG
    GATGATAGGC CTTTCCAAGG GGATGACATT GAGAATTCCC GACTGGCTGC TGAGGTCTAC
    AAAGACATGC CTGAGACCAG CTTTACCCGA ACAATTTCGA ACCCAGAGGT GGTGATGAAG
    CGTCGCAGGC AGCAGAAACT GGAGAGGAGG ATGCAGGAAT TCCGCAGCTC GGATGGGCGG
    CCTGACTCAG GTGGAACATT GAGAATTTAT GCCGATAGTT TAAAACCAAA TATTCCCTAC
    AAGACAATCC TGCTGTCTAC CACAGATCCT GCAGACTTTG CTGTGGCTGA AGCGTTAGAG
    AAATATGGTC TAGAGAAAGA AAATCCTAAG GATTACTGCA TTGCCCGAGT TATGCTTCCT
    CCTGGAGCCC AGCATTCTGA TGAAAAAGGT GCTAAAGAAA TGATCCTGGA TGATGATGAG
    TGTCCTTTAC AAATCTTCAG GGAATGGCCA AGCGACAAAG GGCTTCTAGT CTTTCAGTTG
    AAGAGGAGGC CAGCCGACTA TATTCCAAAG AAAACTAAGA AACACGTCGA TGGGAAGCTG
    GTGAAGGGAA AGGAGAGAGC AGAGGGGTCA GCCTACGGTT CTGCACTCCC TCCCGAGAAA
    CTGCCCTATC TAGTAGAGTT AAGCCCAGAT GGCTCTGACT CCAGAGATAA GCCCAAGCTT
    TACCGCCTTC AGTTGAGCGT TACTGAGGTT GGGACGGAAA AGTTTGATGA GAACTCCATC
    CAGCTGTTCG GATCAGGAAT TCAGCCCCAC CACTGCGACC TCACAAACAT GGATGGAGTT
    GTCACTGTAA CGCCGAGAAG CATGGATGCT GAGACTTACG TGGAAGGACA GCGCATCTCG
    GAGACAACCA TGCTACAGAG CGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG
    TTTGTGGATC CCAGCCAGGA CCACGCTCTG GCAAAGCGAT CTGTGGATGG AAGCCTGACC
    GCCAAGGGTC CCAGACATAA ACCTGGAATT GTTCAAGAGA CAACATTTGA TTTAGGAGGA
    GATGTTCACA GTGGAACAGC ATTACCAACA AGTAAGAGCA CCACTCGGCT GGACGGCGAC
    AGGGTATTGT CTGCCTCCAG CACAGCGGAG CGAGGGATGG TGAAGCCGAT GATGAGAGTA
    GAGCAGCAGC AGGACTACCG CAGGCAAGAA AACAGAACTC AGGATGCTCC TGGGCCTGAG
    CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC
    ATTATAAACT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA
    TATATGGCAT GTCGGTATGT ATTGTCAAGC CAGTACAGAC CCGACGTCAG CCCTACAGAG
    CGCACTCACA AAGTGATTGC AATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT
    CAGAAACAGA AGAATATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAATGC ATCTGAGCTG
    CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CACTGGACGC CCAGGACGTT
    TTAGCACACT TGGTTCAGAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG
    AATAATTACA TGCCAGCCTT CCTGGATGAC CCCGAGGAGA ACAGCCTACA GAGACCAAAA
    ATAGATGATG TTTTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC
    AATGCTGCCC TGACCATCCA GCTCTTCTCT CAGCTCTTCC ACTTTATCAA CATGTGGCTC
    TTCAACAGAT TGGTGACCGA CCCTGAGTCG GGACTGTGCT CCCACTACTG GGGGGCGATT
    ATTCGCCAGC AGCTAGGACA CGTGGAAGCT TGGGCAGAGA AGCAGGGGCT GGAGCTGGCA
    GCGGACTGTC ACCTCAGCAG GATCGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC
    GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAATTC ACTGCAACTT
    CAAGCCTTGT TACAGAATTA CCACTGTGCA CCTGATGAGC CTTTTATTCC CACGGACCTG
    ATAGAGAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGACG AGCTGGCGCG CAGTGATGGC
    CGGGAGGTGC AGCTGGAAGA GGACCCCGAT CTGCAGTTGC CTTTTCTTTT GCCAGAAGAC
    GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTAGACCCT
    TTGTGCCAGA GAGGATTTTG CAGGTTAATC CCTCATGCAC GTTCACCAGG TACTTGGACA
    ATATATTTTG AGGGTGCAGA TTACGAGAGC CATCTTCTGC GTGAGAACAC GGAGCTGGCT
    CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAGAAGCA AAATGGGATG
    GGCCTTAGCA TCGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG
    TCTGTTGTAA AAGGCGGTGC TGCGGATGTG GATGGACGCC TGGCTGCGGG TGACCAGCTC
    CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAGCTCATG
    ACAAGAACAA GTTCTGTGGT GACACTAGAA GTAGCAAAGC AAGGAGCAAT TTATCACGGC
    CTCGCCACGC TGCTTAATCA GCCATCCCCA ATGATGCAAA GAATTTCAGA CCGTCGTGGC
    TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCAAAAC
    GGGTCACCTG AGAGCCCTCA GCTGCCTTGG GCAGAATACA CTGAACCCAA GAAATTGCCT
    GGCGATGACA GACTGATGAA GAACCGGGCT GATCACCGCT CCAGCCCCAA CGTGGCAAAT
    CAGCCTCCTA GCCCTGGAGG GAAAAGCGCA TACGCCCCTG GAACAACAGC TAAGATAACG
    TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCT
    TACCCCATCC CCACTCAGAC ATACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC
    CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG
    CACATGGACA ATAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAGGAGGAG
    CTTCGGGAGG ATAAGGCTTA CCAACTAGAG CGGCATCGGA TCGAGGCAGT CCTGGATCGC
    AAGTCTGATA GCGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCGAG CACTTCCAGC
    CAAGAGCACC TGGACCACCC CTCCAAGGCC GGCACCCCCG CTTCCACTCT GACCAAGAGT
    GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCC TGCCTGTGGC TGGCTCCCAG
    CCGGTCCAAA CTGACCTGCC CCCACCACCC CCGCCGCCCC CCACGCACTA CGCCGGAGAC
    TTCGACGGGA TCCCAACAGA CCTGCCTCTC CCACCACCTC CTGCTAGCCA GACAGGACCT
    GCCTCTGCTC AGGTGGTGGC TGCAGAGAGG AAGAAGAGGG AGGAGCACCA GCGCTGGTAT
    GAGAAGGAGA AGGCCCGTCT AGAGGAGGAG CGGGACAGGA AGCGCCGTGA GCAGGAGCGA
    AAGCTGGGCC AGATGCGCTC CCAGTCACTG AACCCAGCCC CGTTTTCTCC TGTAACTGTC
    CCCCATGTGA AGCCAGAAAA ACCTTCTACA CTCCAGAGGC CCCAAGAGAC GGTCATTCGG
    GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACA
    GTCAGCAAAG AGGAGCTGTC CTCAGGAGAT AGTCTGTCCC CAGACCCGTG GAAGCGGGAT
    GCCCGGGAGA AGCTAGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG
    ATCCAGGAGC TCCAGAATAA GCCCGAGCGC AGTGCAGAGG AAAACGATCG CCTGCGGAAA
    CTCATGCTGG AGTGGCAGTT CCAGAAGAGG CTCCAGGAGT CCAAGCAGAA GGACGAGGAT
    GACGAGGAGG AGGAGGATGA TGACGTGGAT ACCATGCTCA TCATGCAGCG CCTCGAGGCC
    GAGCGAAGAG CAAGGTTACA GGATGAGGAG CGGAGGCGCC AGCAGCAGCT GGAAGAGATG
    CGCAAGCGAG AAGCAGAAGA TCGCGCCAGG CAGGAGGACG AGCGGCGCCG CCAGGAGGAG
    GAGAGAGCAA AGCGAGATGC TGAAGAAAAG AGGCGTCAGG AAGAAGGCTA TTACAGCCGC
    CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGTTGCT GGAGCCGGAG
    GAGCCGGGTC TGTGCAGACC TCCGCTGCCG CGGGGCTACA AGCTCCCGTC CCCGTCCCCT
    CCGGCTGCCG CTCCTCCTCC CCCGCCTCAG CGGACCGCCT CCTCCCTCCA GGCCCAGGCC
    CTCTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG
    GAGCACTGCA GTCTGGCAGG TCAGGACAAG CACTCTGGCA CGAGGAAGTC CCACGGGCAC
    CTTCTTCCCG CCGCCCTTGA GCCGCAGCGG CCCCCCCGCC CGCCGCGCCC GGCCTCCGAC
    GGCATCTTTC TCTGCACCTC ATTCCGGCCC CCCTTGGCCC GAGCCAACAG TACTGCTCAC
    AAAGCAGGCC AGCCCCCACC CCCTCCAAAA AAACCAGGCT TCTACCAGCC CAGCCACTCG
    AAAGGACCAA ACTCTTACCT GGGATCTGCC GGGACAGCTG TTGGCACCCA CGATGCCTAT
    CGGGACCCGA GAGAAAGACT GTCTAAAAGC CACGAGGCAG ATTTACCTGG GAGCTCTGGA
    GCCCCTGAAA ACTTGACATT CAAAGAGCGC CAGCGTCTGT TCTCACAAGG TCAGGACGTG
    TCCAATAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACCAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.