The following IL12A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL12A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OCe45926 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001003293.2 | |
| Accession Version | NM_001003293.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-29 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | interleukin-12 subunit alpha precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03017327.1 and AAEX03017328.1. On Sep 2, 2015 this sequence version replaced NM_001003293.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: U49085.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTGCCCGC CGCGCGGCCT CCTCCTTGTG ACCATCCTGG TCCTGCTAAG CCACCTGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001003293.2 |
| CDS | 1..669 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001003293.2 |
1 | ATGTGCCCGC CGCGCGGCCT CCTCCTTGTG ACCATCCTGG TCCTGCTAAG CCACCTGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe44795 | |
| Clone ID Related Accession (Same CDS sequence) | XM_014110344.2 , XM_014110344.1 | |
| Accession Version | XM_014110344.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | interleukin-12 subunit alpha isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAGGTA GAGGCAGGCC AAACCAAGCT GACAAGCTCA GCCTGCTCCT TGCTTCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_013965819.1 |
| CDS | 535..1251 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_014110344.2 |
1 | ATGGAAGGTA GAGGCAGGCC AAACCAAGCT GACAAGCTCA GCCTGCTCCT TGCTTCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe44795 | |
| Clone ID Related Accession (Same CDS sequence) | XM_014110344.2 , XM_014110344.1 | |
| Accession Version | XM_014110344.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | interleukin-12 subunit alpha isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAGGTA GAGGCAGGCC AAACCAAGCT GACAAGCTCA GCCTGCTCCT TGCTTCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_013965819.1 |
| CDS | 513..1229 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_014110344.1 |
1 | ATGGAAGGTA GAGGCAGGCC AAACCAAGCT GACAAGCTCA GCCTGCTCCT TGCTTCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis. |
Cytokine gene expression in the tissues of dogs infected by Leishmania infantum. |
Detection, cDNA cloning and sequencing of canine interleukin 12. |
