ABCB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCB1
Entrez Gene ID	403879
Full Name	ATP binding cassette subfamily B member 1
Synonyms	MDR1,p-gp
General protein information	Preferred Names ATP binding cassette subfamily B member 1 Names multidrug resistance protein 1 multidrug resistance p-glycoprotein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003215.2	NP_001003215.2	multidrug resistance protein 1
XM_014118874.1	XP_013974349.1	multidrug resistance protein 1 isoform X1
XM_014118873.1	XP_013974348.1	multidrug resistance protein 1 isoform X1
XM_014118873.2	XP_013974348.1	multidrug resistance protein 1 isoform X1
XM_014118874.2	XP_013974349.1	multidrug resistance protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ABCB1	XP_001163417.1
Mus musculus (house mouse)	Abcb1a	NP_035206.2
Rattus norvegicus (Norway rat)	Abcb1a	NP_596892.1
Caenorhabditis elegans (roundworm)	pgp-9	NP_507487.1
Arabidopsis thaliana (thale cress)	PGP9	NP_193539.6
	ABCB1	NP_001028059.1
Bos taurus (cattle)	ABCB1	XP_002686777.3
Drosophila melanogaster (fruit fly)	Mdr50	NP_523740.3
Arabidopsis thaliana (thale cress)	PGP5	NP_192092.1
Oryza sativa (rice)	Os01g0695700	NP_001043962.1
Xenopus tropicalis (tropical clawed frog)	abcb1	XP_004921510.1
Caenorhabditis elegans (roundworm)	pgp-1	NP_502413.1
Schizosaccharomyces pombe (fission yeast)	pmd1	NP_588265.1
Arabidopsis thaliana (thale cress)	PGP11	NP_171753.1
Arabidopsis thaliana (thale cress)	PGP7	NP_199466.1
Arabidopsis thaliana (thale cress)	ABCB4	NP_182223.1
Arabidopsis thaliana (thale cress)	PGP12	NP_171754.1
Homo sapiens (human)	ABCB1	NP_000918.2
Canis lupus familiaris (dog)	ABCB1	NP_001003215.1
Magnaporthe oryzae (rice blast fungus)	MGG_00141	XP_003718990.1
Neurospora crassa	NCU07546	XP_959059.2
Arabidopsis thaliana (thale cress)	PGP21	NP_191774.2
Arabidopsis thaliana (thale cress)	PGP3	NP_192091.1

Gene Ontology
Bibliography

Related articles in PubMed

Immunohistochemical Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Hyperplasia, Neoplasia and Supporting Stroma.
Levi M, Brunetti B, Sarli G, Benazzi C
Journal of comparative pathology155(4)277-285(2016 Nov)

The prevalence of ABCB1:c.227_230delATAG mutation in affected dog breeds from European countries.
Firdova Z, Turnova E, Bielikova M, Turna J, Dudas A
Research in veterinary science10689-92(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Malignant and invasive tumours were more likely to express PGP and/or BCRP in luminal and stromal components and evaluation of these markers could provide valuable information for the identification of tumours characterized by an aggressive and chemoresistant phenotype.
Title: Immunohistochemical Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Hyperplasia, Neoplasia and Supporting Stroma.

The genetics of the MDR1 mutation that causes hypersensitivity to macrocyclic lactones was studied in predisposed dog breeds in multiple European countries.
Title: The prevalence of ABCB1:c.227_230delATAG mutation in affected dog breeds from European countries.

In ABCB1 mut/mut dogs acepromazine dose rates should be reduced and careful monitoring performed during sedation.
Title: The Effect of the Canine ABCB1-1? Mutation on Sedation after Intravenous Administration of Acepromazine.

Specific efflux mediated by hPGP or hBCRP can be determined with MDCKII cells where cPgp has been knocked out by zinc finger nucleases.
Title: Genomic Knockout of Endogenous Canine P-Glycoprotein in Wild-Type, Human P-Glycoprotein and Human BCRP Transfected MDCKII Cell Lines by Zinc Finger Nucleases.

These results indicate that P-glycoprotein expression is present in nearly one third of newly diagnosed canine lymphomas of different morphological subtypes including those most commonly occurring, such as cenroblastic lymphomas.
Title: Immunohistochemical detection of P-glycoprotein in various subtypes of canine lymphomas.

The following ABCB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe39859	NM_001003215.2 Latest version!	Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe39859	XM_014118873.2 Latest version!	Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe39859	XM_014118874.2 Latest version!	Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OCe39859	XM_014118873.1	Canis lupus familiaris ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe39859	XM_014118874.1	Canis lupus familiaris ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCB1 ( NM_001003215.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003215.2 Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), mRNA.

Price & Availability↑

CloneID	OCe39859
Clone ID Related Accession (Same CDS sequence)	NM_001003215.2 , XM_014118874.1 , XM_014118873.1 , XM_014118873.2 , XM_014118874.2
Accession Version	NM_001003215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Canis lupus familiaris(dog)
Product	multidrug resistance protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ068953.1, AF536758.1 and DN877923.1. On Sep 4, 2015 this sequence version replaced NM_001003215.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY582533.1, AF536758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003215.2
CDS	1..3846
Translation	MDPEGGRKGSAEKNFWKMGKKSKKNEKKEKKPTVSTFAMFRYSNWLDRLYMLVGTMAAIIHGAALPLMMLVFGNMTDSFANAGISRNKTFPVIINESITNNTQHFINHLEEEMTTYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQILKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSIATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAIWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTSLVLSSEYSIGQVLTVFFSVLIGAFSIGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVCIDGQDIRTINVRHLREITGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTRGNEIELENATGESKSESDALEMSPKDSGSSLIKRRSTRRSIHAPQGQDRKLGTKEDLNENVPPVSFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSIIFSRIIGIFTRDEDPETKRQNSNMFSVLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGAGKIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGVSFSITQAMMYFSYAGCFRFGAYLVANEFMNFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKSPLIDSYSPHGLKPNTLEGNVTFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGSVLIDGKEIKHLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIMQAAKEANIHHFIETLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQAGAKR

Target ORF information:

RefSeq Version	NM_001003215.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003215.2

ORF Insert Sequence:

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( XM_014118874.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974349.1 Canis lupus familiaris ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe39859
Clone ID Related Accession (Same CDS sequence)	NM_001003215.2 , XM_014118874.1 , XM_014118873.1 , XM_014118873.2 , XM_014118874.2
Accession Version	XM_014118874.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	multidrug resistance protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974349.1
CDS	118..3963
Translation	MDPEGGRKGSAEKNFWKMGKKSKKNEKKEKKPTVSTFAMFRYSNWLDRLYMLVGTMAAIIHGAALPLMMLVFGNMTDSFANAGISRNKTFPVIINESITNNTQHFINHLEEEMTTYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQILKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSIATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAIWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTSLVLSSEYSIGQVLTVFFSVLIGAFSIGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVCIDGQDIRTINVRHLREITGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTRGNEIELENATGESKSESDALEMSPKDSGSSLIKRRSTRRSIHAPQGQDRKLGTKEDLNENVPPVSFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSIIFSRIIGIFTRDEDPETKRQNSNMFSVLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGAGKIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGVSFSITQAMMYFSYAGCFRFGAYLVANEFMNFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKSPLIDSYSPHGLKPNTLEGNVTFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGSVLIDGKEIKHLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIMQAAKEANIHHFIETLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQAGAKR

Target ORF information:

RefSeq Version	XM_014118874.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118874.1

ORF Insert Sequence:

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( XM_014118873.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974348.1 Canis lupus familiaris ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1), transcript variant X1, mRNA.

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CloneID	OCe39859
Clone ID Related Accession (Same CDS sequence)	NM_001003215.2 , XM_014118874.1 , XM_014118873.1 , XM_014118873.2 , XM_014118874.2
Accession Version	XM_014118873.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	multidrug resistance protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974348.1
CDS	162..4007
Translation	MDPEGGRKGSAEKNFWKMGKKSKKNEKKEKKPTVSTFAMFRYSNWLDRLYMLVGTMAAIIHGAALPLMMLVFGNMTDSFANAGISRNKTFPVIINESITNNTQHFINHLEEEMTTYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQILKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSIATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAIWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTSLVLSSEYSIGQVLTVFFSVLIGAFSIGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVCIDGQDIRTINVRHLREITGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTRGNEIELENATGESKSESDALEMSPKDSGSSLIKRRSTRRSIHAPQGQDRKLGTKEDLNENVPPVSFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSIIFSRIIGIFTRDEDPETKRQNSNMFSVLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGAGKIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGVSFSITQAMMYFSYAGCFRFGAYLVANEFMNFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKSPLIDSYSPHGLKPNTLEGNVTFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGSVLIDGKEIKHLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIMQAAKEANIHHFIETLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQAGAKR

Target ORF information:

RefSeq Version	XM_014118873.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118873.1

ORF Insert Sequence:

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( XM_014118873.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974348.1 Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe39859
Clone ID Related Accession (Same CDS sequence)	NM_001003215.2 , XM_014118874.1 , XM_014118873.1 , XM_014118873.2 , XM_014118874.2
Accession Version	XM_014118873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	multidrug resistance protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974348.1
CDS	174..4019
Translation	MDPEGGRKGSAEKNFWKMGKKSKKNEKKEKKPTVSTFAMFRYSNWLDRLYMLVGTMAAIIHGAALPLMMLVFGNMTDSFANAGISRNKTFPVIINESITNNTQHFINHLEEEMTTYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQILKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSIATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAIWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTSLVLSSEYSIGQVLTVFFSVLIGAFSIGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVCIDGQDIRTINVRHLREITGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTRGNEIELENATGESKSESDALEMSPKDSGSSLIKRRSTRRSIHAPQGQDRKLGTKEDLNENVPPVSFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSIIFSRIIGIFTRDEDPETKRQNSNMFSVLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGAGKIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGVSFSITQAMMYFSYAGCFRFGAYLVANEFMNFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKSPLIDSYSPHGLKPNTLEGNVTFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGSVLIDGKEIKHLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIMQAAKEANIHHFIETLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQAGAKR

Target ORF information:

RefSeq Version	XM_014118873.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118873.2

ORF Insert Sequence:

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( XM_014118874.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013974349.1 Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe39859
Clone ID Related Accession (Same CDS sequence)	NM_001003215.2 , XM_014118874.1 , XM_014118873.1 , XM_014118873.2 , XM_014118874.2
Accession Version	XM_014118874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	multidrug resistance protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974349.1
CDS	104..3949
Translation	MDPEGGRKGSAEKNFWKMGKKSKKNEKKEKKPTVSTFAMFRYSNWLDRLYMLVGTMAAIIHGAALPLMMLVFGNMTDSFANAGISRNKTFPVIINESITNNTQHFINHLEEEMTTYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQILKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSIATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAIWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTSLVLSSEYSIGQVLTVFFSVLIGAFSIGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVCIDGQDIRTINVRHLREITGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTRGNEIELENATGESKSESDALEMSPKDSGSSLIKRRSTRRSIHAPQGQDRKLGTKEDLNENVPPVSFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSIIFSRIIGIFTRDEDPETKRQNSNMFSVLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGAGKIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGVSFSITQAMMYFSYAGCFRFGAYLVANEFMNFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKSPLIDSYSPHGLKPNTLEGNVTFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGSVLIDGKEIKHLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIMQAAKEANIHHFIETLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQAGAKR

Target ORF information:

RefSeq Version	XM_014118874.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ATP binding cassette subfamily B member 1 (ABCB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118874.2

ORF Insert Sequence:

ATGGATCCTG AAGGAGGCCG TAAGGGGAGT GCAGAGAAGA ACTTCTGGAA AATGGGCAAA 
AAAAGTAAAA AAAATGAGAA GAAAGAAAAG AAACCAACTG TCAGCACGTT TGCAATGTTT 
CGCTATTCAA ATTGGCTTGA TAGGTTGTAT ATGTTGGTGG GGACAATGGC TGCCATCATC 
CATGGAGCTG CACTCCCTCT CATGATGCTG GTTTTTGGAA ACATGACAGA TAGCTTTGCA 
AATGCAGGAA TTTCAAGAAA CAAAACTTTT CCAGTTATAA TTAATGAAAG TATTACGAAC 
AATACACAAC ATTTCATCAA CCATCTGGAG GAGGAAATGA CCACGTATGC CTATTATTAC 
AGTGGGATCG GTGCTGGCGT GCTGGTGGCT GCTTACATCC AGGTTTCATT CTGGTGCCTG 
GCAGCAGGAA GACAGATACT CAAAATTAGA AAACAATTTT TTCATGCTAT CATGCGACAG 
GAGATTGGCT GGTTTGACGT GCATGACGTT GGGGAGCTTA ACACCCGGCT CACAGACGAT 
GTCTCCAAAA TCAATGAAGG AATTGGCGAC AAAATTGGAA TGTTCTTTCA ATCAATAGCA 
ACATTTTTCA CCGGTTTTAT AGTGGGGTTT ACACGTGGTT GGAAGCTAAC CCTTGTGATT 
TTGGCCATCA GCCCTGTTCT TGGACTTTCA GCCGCCATCT GGGCAAAGAT ACTATCTTCA 
TTTACTGATA AAGAACTCTT GGCCTATGCA AAAGCTGGAG CAGTAGCTGA AGAAGTCTTA 
GCAGCAATCA GAACTGTGAT TGCCTTTGGA GGACAAAAGA AAGAACTTGA AAGGTACAAC 
AAAAATTTAG AAGAAGCTAA AAGAATTGGG ATAAAGAAAG CTATCACGGC CAACATTTCT 
ATTGGTGCCG CTTTCTTATT GATCTATGCA TCATATGCTC TGGCTTTCTG GTATGGGACC 
TCCTTGGTCC TCTCCAGTGA ATATTCTATT GGACAAGTAC TCACTGTCTT CTTTTCTGTA 
TTAATTGGGG CTTTTAGTAT TGGACAGGCA TCCCCAAGCA TTGAAGCATT TGCAAACGCA 
AGAGGAGCAG CTTATGAAAT CTTCAAGATA ATTGACAATA AACCAAGCAT TGACAGCTAT 
TCGAAGAGTG GACATAAACC AGATAATATT AAGGGAAATT TGGAATTCAA AAATGTTCAC 
TTCAGTTACC CTTCTCGAAA AGAAGTTAAG ATCTTAAAGG GTCTCAACCT GAAGGTTCAG 
AGTGGGCAGA CAGTGGCGCT GGTTGGGAAC AGTGGCTGCG GGAAGAGCAC GACCGTGCAG 
CTGATGCAGA GGCTCTATGA CCCCACAGAT GGCATGGTCT GTATTGATGG ACAGGACATT 
AGGACCATAA ATGTAAGGCA TCTTCGGGAA ATTACTGGTG TGGTGAGTCA GGAGCCTGTG 
TTGTTTGCCA CCACGATAGC TGAAAACATT CGCTATGGCC GCGAAAATGT CACCATGGAT 
GAGATTGAGA AAGCTGTTAA GGAAGCCAAT GCCTATGATT TTATCATGAA ACTACCTAAT 
AAATTTGACA CTCTGGTTGG AGAGAGAGGG GCCCAGCTGA GTGGTGGACA GAAACAGAGA 
ATCGCCATTG CTCGGGCCCT GGTTCGCAAC CCCAAGATTC TTCTGCTGGA TGAGGCAACG 
TCAGCTCTGG ACACTGAAAG TGAAGCAGTG GTTCAGGTGG CCCTGGATAA GGCCAGAAAA 
GGCCGGACTA CCATTGTGAT AGCTCATCGT TTGTCTACAG TTCGTAATGC CGATGTCATT 
GCTGGTTTTG ATGATGGAGT CATTGTGGAG AAAGGAAATC ATGATGAACT CATGAAAGAG 
AAGGGCATTT ACTTCAAACT TGTCACAATG CAGACAAGAG GAAATGAAAT TGAGTTAGAA 
AATGCCACTG GTGAATCCAA AAGTGAAAGT GATGCCTTGG AAATGTCTCC AAAAGATTCA 
GGGTCCAGTT TAATAAAAAG AAGATCAACT CGCAGGAGTA TACATGCACC ACAAGGCCAA 
GACAGAAAGC TTGGTACAAA AGAGGACTTG AATGAGAATG TACCTCCAGT TTCCTTCTGG 
AGGATTCTGA AGCTGAACTC AACTGAATGG CCTTATTTTG TGGTTGGTAT ATTTTGTGCT 
ATTATAAACG GAGGCCTGCA ACCAGCATTT TCAATAATAT TTTCAAGGAT TATAGGGATC 
TTTACCCGAG ATGAGGATCC TGAAACAAAA CGACAGAATA GTAACATGTT TTCTGTATTG 
TTTCTAGTCC TTGGAATTAT TTCTTTTATT ACATTTTTCC TCCAGGGCTT CACATTTGGC 
AAAGCTGGGG AGATCCTCAC TAAGCGGCTT CGATACATGG TTTTCAGATC CATGCTGAGA 
CAGGATGTCA GCTGGTTTGA TGACCCTAAA AACACCACTG GAGCATTGAC AACCAGGCTT 
GCCAATGATG CGGCTCAAGT TAAAGGGGCT ATAGGTTCCA GGCTTGCTGT CATTACCCAG 
AATATAGCAA ATCTTGGGAC AGGCATTATT ATATCCTTAA TCTATGGTTG GCAATTAACA 
CTTTTACTCT TAGCAATTGT ACCCATCATT GCAATAGCAG GAGTTGTTGA AATGAAAATG 
TTGTCTGGAC AAGCACTGAA AGATAAGAAA GAGCTAGAAG GAGCTGGGAA GATTGCTACA 
GAAGCCATCG AAAACTTCCG AACTGTTGTT TCTTTGACTC GGGAGCAGAA GTTTGAATAC 
ATGTATGCAC AGAGTTTGCA AGTACCATAC AGAAACTCTT TGAGGAAAGC ACACATCTTC 
GGGGTCTCAT TTTCTATCAC CCAGGCAATG ATGTATTTTT CCTATGCTGG CTGTTTCCGG 
TTTGGTGCCT ACTTGGTGGC AAATGAGTTC ATGAACTTTC AGGATGTTCT TTTGGTATTC 
TCAGCTATTG TCTTTGGTGC CATGGCAGTG GGGCAGGTCA GTTCATTTGC TCCTGACTAT 
GCCAAAGCCA AAGTATCAGC AGCCCACGTC ATCATGATCA TTGAAAAAAG CCCTCTGATT 
GACAGCTACA GCCCTCACGG CCTCAAGCCA AATACGTTGG AAGGAAATGT GACATTTAAT 
GAGGTCGTGT TCAACTATCC CACTCGACCA GACATCCCCG TGCTCCAGGG GCTGAGCCTC 
GAGGTGAAGA AGGGCCAGAC GCTGGCCCTC GTAGGTAGCA GTGGCTGTGG GAAGAGCACA 
GTTGTTCAGC TCCTAGAGCG CTTCTATGAC CCCTTGGCTG GTTCAGTGCT AATTGATGGC 
AAAGAGATAA AGCACCTGAA TGTCCAGTGG CTCCGAGCAC ACCTGGGCAT CGTGTCTCAG 
GAGCCCATCC TGTTTGACTG CAGCATTGCC GAGAACATTG CCTATGGAGA CAACAGCCGG 
GTCGTATCAC ATGAAGAGAT TATGCAGGCA GCCAAGGAGG CCAACATACA CCACTTCATC 
GAGACACTCC CTGAGAAATA CAACACCAGA GTAGGAGACA AAGGAACCCA GCTCTCTGGT 
GGCCAGAAAC AGCGCATTGC CATAGCTCGC GCTCTTGTTA GACAGCCTCA TATTTTGCTT 
TTGGATGAAG CTACATCAGC TCTGGATACA GAAAGTGAAA AGGTTGTCCA AGAAGCCCTG 
GACAAAGCCA GAGAAGGCCG CACCTGCATT GTGATCGCCC ACCGCTTGTC CACCATCCAG 
AATGCAGATT TAATAGTGGT GTTTCAGAAT GGCAAAGTCA AGGAGCATGG CACACATCAA 
CAGCTGCTGG CTCAGAAAGG CATCTATTTT TCCATGGTCA GTGTCCAGGC TGGAGCAAAG 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Immunohistochemical Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Hyperplasia, Neoplasia and Supporting Stroma.
Journal of comparative pathology155(4)277-285(2016 Nov)
Levi M,Brunetti B,Sarli G,Benazzi C

The prevalence of ABCB1:c.227_230delATAG mutation in affected dog breeds from European countries.
Research in veterinary science10689-92(2016 Jun)
Firdova Z,Turnova E,Bielikova M,Turna J,Dudas A

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オンライン注文

ABCB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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