TJP2 cDNA ORF clone, Canis lupus familiaris(dog)

The following TJP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe00336 NM_001003204.1
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01211 XM_014113984.1
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01215 XM_005615721.3
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01216 XM_005615722.2
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01217 XM_005615725.3
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01217 XM_022416196.1
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01218 XM_005615724.3
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe285678 XM_022415947.1
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe34545 XM_005615719.2
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe34545 XM_022414943.1
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe34546 XM_022415227.1
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe34546 XM_005615720.2
Latest version!
Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OCe01218 XM_005615724.2 Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe01217 XM_005615725.2 Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe01215 XM_005615721.2 Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe00336
    Clone ID Related Accession (Same CDS sequence) NM_001003204.1
    Accession Version NM_001003204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-22
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L27152.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27152.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GACCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGCAAATGAC CAGAACCGCT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001003204.1
    CDS398..3922
    Translation

    Target ORF information:

    RefSeq Version NM_001003204.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001003204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GACCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGCAAATGAC CAGAACCGCT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01211
    Clone ID Related Accession (Same CDS sequence) XM_014113984.1
    Accession Version XM_014113984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGGTACA AGAAGTACAT GGCCATCCTG GAAGCCGCAG TGGGTATCAC CCCACTTAAC 
    AAAAGAGAGC TTCTTCCTGA GAGCAGAAGA CATGTGAATC TCTGGCAGCA CCCCGGGGTG
    CGGAGTACCA CGTTATTATC GTCTCCCTGC GAGTGGGATT GGCCCTTCGG CTGTACCTCC
    TGGCACGAGC ATTCCACAGA GCACAGACGT GCGCTGGCAG GCAGCCTGCT TTTCCAGGCC
    CCAGGCATGG AAGAACTGAT ATGGGAGCAG TACACTGTGA CCCTACAAAA GGATTCCAAA
    AGAGGATTTG GAATTGCAGT GTCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGAGAA
    ACGTCAATCG TCATTTCTGA CGTGCTACCG GGTGGGCCTG CTGATGGGCT GCTTCAAGAA
    AATGACAGGG TGGTCATGGT TAACGGCACC CCCATGGAGG ATGTGCTCCA TTCATTTGCT
    GTCCAGCAGC TGAGAAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCCCCA GGGCAGCCTA CCCGTCGATG AGGATGACCG TGCTTTTGAG
    GTGATGGATG AGTTCGATGG CAGAAGTGCC CGCAGCGGGT ACAGCGAGAG GAGCCGGCGC
    AGCAGCCACG GTGGGCGCAG CCGCAGCTGG GAGGACAGCC CGGAGAGGGG GCGTCCCCAT
    GAGCGGGCGT GGAGCCAGGA GCGCGAGCGC AGCCGTGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACCG GCGGCCTCGA GAGCGCAGCC GGGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ATGACGATGA CTACGGGCGG CCCGGAGAGC GCAGCCACGG CATGAGCACT
    GACAGGGGCT ACGACCGTGG CTACGACCGT GGCTACGACC GTGGCTACGA CCGCACCTAT
    AGCCCAGAGG CCGAGTATGG CCGCAGGGCC CAGCCTGATG CCCGGCACGC AGGGTCCCGG
    AGCCGCAGCC GCGAGCACTT GCGCTCCCGC AGCCCCAGCC CTGAGCTGAG GGGGCGGCCG
    GACCATGCAG GCCAGCCTGA CTCGGACAGG CCCATCGGGG TCCTTCTGAT GAAAAGCAAA
    GCAAATGAAG AGTACGGTCT CCGGCTTGGG AGTCAGATCT TCATAAAGGA AATGACCAGA
    ACCGGTCTGG CAACTAAAGA TGGCAACCTG CACGAAGGAG ACATAATTCT CAAGATAAAT
    GGAACTGTAA CTGAGAACAT GTCTTTAACT GATGCCCGAA AACTGATAGA AAAGTCAAGA
    GGAAAACTCC AACTAGTGGT GTTGAGAGAC AGCAAGCAAA CGCTCATCAA CATTCCATCA
    TTAAATGACA GCGACTCTGA AATAGAAGAT ATCTCAGAAA TAGAGTCAAA CCGATCATTT
    TCTCCAGAGG AGAGACGGCA GCAGTATTCC GACTATGATT ATCATTCCTC AAATGAAAAA
    CTGAAGGAAA GGCCAAATTC AAGAGAGGAC ATGCAGAACA GATGGTCCAG GATGGGCGCC
    ACACCCACTC CCTTTAAGTC CATGGGGGAT ATTGCATCTG TGGTTGGCAC AGAGAACAGC
    AAGGAACCCA GATACCAAGA GGAACCACCA GCTCCTCAAC CTAAAGCAGC CCCAAGAACT
    TTTCTTCGCC CGAGTCCTGA AGATGAAGCA ATATATGGTC CCAATACCAA AATGGTGAGG
    TTTAAGAAGG GAGACAGCGT GGGACTCCGA TTGGCTGGTG GCAATGACGT TGGGATATTT
    GTGGCTGGTA TTCAAGAGGG TACCTCGGCA GAACAAGAAG GCCTTCAAGA GGGGGACCAG
    ATTCTGAAGG TGAACACACA GGATTTCAGA GGGCTAGTTC GGGAAGATGC CGTTCTCTAC
    CTGCTAGAAA TCCCTAAAGG TGAAATGGTG ACCATTTTAG CTCAGAGCCG AGCTGATGTG
    TATAGAGACA TCCTGGCTTG TGGCAGAGGG GACTCATTTT TTATAAGAAG CCACTTTGAA
    TGTGAGAAGG AAACTCCACA GAGCCTGGCC TTCAGCAGGG GGGAGGTCTT CCGAGTTGTA
    GATACACTGT ATGATGGCAA GCTGGGCCAC TGGCTGGCTG TGAGGATTGG AAACGAATTG
    GAGAAGGGCT TAATCCCCAA CAAAAGCAGA GCTGAACAAA TGGCCAGTGT TCAGAATGCC
    CAGAGAGACA ATGCTGGGGA CAGAGCAGAT TTCTGGAGAA TGCGTGGCCA GAGATCTGGC
    ATGAAGAAGA ACTTAAGGAA AAGTCGGGAA GATCTAACAG CTGCTGTGTC AGTTAGCACC
    AAGTTCCCAG CTTACGAAAG GGTTTTGCTG CGAGAAGCTG GTTTCAAGAG ACCTGTGGTC
    TTATTTGGCC CTATAGCAGA TATAGCCTTG GAAAAGTTGG CAAATGAGTT ACCGGACCTG
    TTCCAAACTG CTAAAACGGA ACCAAAAGAT GCAGGATCCG AGAAATCTAG TGGGGTGGTC
    CGGTTAAATA CTGTGAGGCA AATTATTGAA CAGGATAAGC ATGCACTATT GGATGTGACT
    CCTAAAGCTG TGGACCTGTT GAATTATACT CAGTGGTTCC CGATTGTGAT TTTTTTCAAC
    CCAGACTCTA GACAAGGTGT CAAAACCATG AGACAGAGGT TGAATCCAAC ATCCAACAAA
    AGTTCTCGGA AGTTGTATGA TCAAGCCAAC AAGCTTAAGA AAACGTGTGC ACATCTTTTT
    ACAGCTACTA TCAACCTAAA TTCAGCCAAT GATAGCTGGT TTGGCAGCTT GAAGGACACA
    ATTCAGCATC AGCAAGGAGA AGCAGTTTGG GTCTCTGAAG GAAAGATGGA AGGGATGGAT
    GATGACCCTG AAGACCGCAT GTCCTACTTA ACTGCCATGG GCGCAGACTA TCTGAGTTGC
    GACAGCCGCC TCATCAGTGA CTTTGAAGAC ACCGACGGTG AAGGAGGTGC CTACACTGAC
    AATGAGCTGG ATGAGCCAGC TGAGGAGCCA CTGGTGTCTT CCATCACCCG CTCCTCGGAG
    CCGGTGCAGC ATGAGGAGAG CATAAGGAAG CCCAGCCCAG AGCCACGAGC TCAGATGAGG
    AGGGCTGCTA GCAGAGATCA ACTTAGGGAC AGTAGTCCAC CCCCCGCATT CAAGCCAGAG
    CCGCCCAAGG CCAAAACTCA GAACAGAGAA GAATCCTTTG ACATCTCCAG ATCCCATGAC
    TATAAGTCAA ACCCCTCAGC CGTTGCTGGT AATGAAGTTT CTGGGGCATC TACCAGAAGC
    TGTCCTCCTC CTATTGCAGC AAAACCTTCC TTTGGAAGGT CTATACTGAA GCCCTCCACT
    CCTGTCCCTT CCCCAGAGAG TGAGGAGGTG GGAGAGGGCA GTGAAGAGCA AGAAGGCGCT
    CCCAAGTCTG TCCTGGGCAA AGTTAAAATA TTTGAGAAGA TGGATCACAA GGCAAGATTA
    CAGAGAATGC AGGAGCTTCA AGAAGCACAG AATGCAAGGA TTGAAATTGC TCAGAAGCAT
    CCTGACATCT ATGCAGTTCC AATCAAAACA CACAAGCCAG ACCCTGGCCT GTCCCAGCAC
    ACAAGTTCCA GGCCACCCGA GCCCCAGAAA GGCCCTTCCA GGCTCTACCA GGATCCCAGA
    GGAAGTTATG GCAGTGATGC TGAAGAGGAA GAATACCGCC AGCAGCTGTC CGAACACTCG
    AAGCGTGGCT ATTACAGCCA GCCTTCCCGG TACCGGGACA CGGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969459.1
    CDS147..3917
    Translation

    Target ORF information:

    RefSeq Version XM_014113984.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGGTACA AGAAGTACAT GGCCATCCTG GAAGCCGCAG TGGGTATCAC CCCACTTAAC 
    AAAAGAGAGC TTCTTCCTGA GAGCAGAAGA CATGTGAATC TCTGGCAGCA CCCCGGGGTG
    CGGAGTACCA CGTTATTATC GTCTCCCTGC GAGTGGGATT GGCCCTTCGG CTGTACCTCC
    TGGCACGAGC ATTCCACAGA GCACAGACGT GCGCTGGCAG GCAGCCTGCT TTTCCAGGCC
    CCAGGCATGG AAGAACTGAT ATGGGAGCAG TACACTGTGA CCCTACAAAA GGATTCCAAA
    AGAGGATTTG GAATTGCAGT GTCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGAGAA
    ACGTCAATCG TCATTTCTGA CGTGCTACCG GGTGGGCCTG CTGATGGGCT GCTTCAAGAA
    AATGACAGGG TGGTCATGGT TAACGGCACC CCCATGGAGG ATGTGCTCCA TTCATTTGCT
    GTCCAGCAGC TGAGAAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCCCCA GGGCAGCCTA CCCGTCGATG AGGATGACCG TGCTTTTGAG
    GTGATGGATG AGTTCGATGG CAGAAGTGCC CGCAGCGGGT ACAGCGAGAG GAGCCGGCGC
    AGCAGCCACG GTGGGCGCAG CCGCAGCTGG GAGGACAGCC CGGAGAGGGG GCGTCCCCAT
    GAGCGGGCGT GGAGCCAGGA GCGCGAGCGC AGCCGTGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACCG GCGGCCTCGA GAGCGCAGCC GGGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ATGACGATGA CTACGGGCGG CCCGGAGAGC GCAGCCACGG CATGAGCACT
    GACAGGGGCT ACGACCGTGG CTACGACCGT GGCTACGACC GTGGCTACGA CCGCACCTAT
    AGCCCAGAGG CCGAGTATGG CCGCAGGGCC CAGCCTGATG CCCGGCACGC AGGGTCCCGG
    AGCCGCAGCC GCGAGCACTT GCGCTCCCGC AGCCCCAGCC CTGAGCTGAG GGGGCGGCCG
    GACCATGCAG GCCAGCCTGA CTCGGACAGG CCCATCGGGG TCCTTCTGAT GAAAAGCAAA
    GCAAATGAAG AGTACGGTCT CCGGCTTGGG AGTCAGATCT TCATAAAGGA AATGACCAGA
    ACCGGTCTGG CAACTAAAGA TGGCAACCTG CACGAAGGAG ACATAATTCT CAAGATAAAT
    GGAACTGTAA CTGAGAACAT GTCTTTAACT GATGCCCGAA AACTGATAGA AAAGTCAAGA
    GGAAAACTCC AACTAGTGGT GTTGAGAGAC AGCAAGCAAA CGCTCATCAA CATTCCATCA
    TTAAATGACA GCGACTCTGA AATAGAAGAT ATCTCAGAAA TAGAGTCAAA CCGATCATTT
    TCTCCAGAGG AGAGACGGCA GCAGTATTCC GACTATGATT ATCATTCCTC AAATGAAAAA
    CTGAAGGAAA GGCCAAATTC AAGAGAGGAC ATGCAGAACA GATGGTCCAG GATGGGCGCC
    ACACCCACTC CCTTTAAGTC CATGGGGGAT ATTGCATCTG TGGTTGGCAC AGAGAACAGC
    AAGGAACCCA GATACCAAGA GGAACCACCA GCTCCTCAAC CTAAAGCAGC CCCAAGAACT
    TTTCTTCGCC CGAGTCCTGA AGATGAAGCA ATATATGGTC CCAATACCAA AATGGTGAGG
    TTTAAGAAGG GAGACAGCGT GGGACTCCGA TTGGCTGGTG GCAATGACGT TGGGATATTT
    GTGGCTGGTA TTCAAGAGGG TACCTCGGCA GAACAAGAAG GCCTTCAAGA GGGGGACCAG
    ATTCTGAAGG TGAACACACA GGATTTCAGA GGGCTAGTTC GGGAAGATGC CGTTCTCTAC
    CTGCTAGAAA TCCCTAAAGG TGAAATGGTG ACCATTTTAG CTCAGAGCCG AGCTGATGTG
    TATAGAGACA TCCTGGCTTG TGGCAGAGGG GACTCATTTT TTATAAGAAG CCACTTTGAA
    TGTGAGAAGG AAACTCCACA GAGCCTGGCC TTCAGCAGGG GGGAGGTCTT CCGAGTTGTA
    GATACACTGT ATGATGGCAA GCTGGGCCAC TGGCTGGCTG TGAGGATTGG AAACGAATTG
    GAGAAGGGCT TAATCCCCAA CAAAAGCAGA GCTGAACAAA TGGCCAGTGT TCAGAATGCC
    CAGAGAGACA ATGCTGGGGA CAGAGCAGAT TTCTGGAGAA TGCGTGGCCA GAGATCTGGC
    ATGAAGAAGA ACTTAAGGAA AAGTCGGGAA GATCTAACAG CTGCTGTGTC AGTTAGCACC
    AAGTTCCCAG CTTACGAAAG GGTTTTGCTG CGAGAAGCTG GTTTCAAGAG ACCTGTGGTC
    TTATTTGGCC CTATAGCAGA TATAGCCTTG GAAAAGTTGG CAAATGAGTT ACCGGACCTG
    TTCCAAACTG CTAAAACGGA ACCAAAAGAT GCAGGATCCG AGAAATCTAG TGGGGTGGTC
    CGGTTAAATA CTGTGAGGCA AATTATTGAA CAGGATAAGC ATGCACTATT GGATGTGACT
    CCTAAAGCTG TGGACCTGTT GAATTATACT CAGTGGTTCC CGATTGTGAT TTTTTTCAAC
    CCAGACTCTA GACAAGGTGT CAAAACCATG AGACAGAGGT TGAATCCAAC ATCCAACAAA
    AGTTCTCGGA AGTTGTATGA TCAAGCCAAC AAGCTTAAGA AAACGTGTGC ACATCTTTTT
    ACAGCTACTA TCAACCTAAA TTCAGCCAAT GATAGCTGGT TTGGCAGCTT GAAGGACACA
    ATTCAGCATC AGCAAGGAGA AGCAGTTTGG GTCTCTGAAG GAAAGATGGA AGGGATGGAT
    GATGACCCTG AAGACCGCAT GTCCTACTTA ACTGCCATGG GCGCAGACTA TCTGAGTTGC
    GACAGCCGCC TCATCAGTGA CTTTGAAGAC ACCGACGGTG AAGGAGGTGC CTACACTGAC
    AATGAGCTGG ATGAGCCAGC TGAGGAGCCA CTGGTGTCTT CCATCACCCG CTCCTCGGAG
    CCGGTGCAGC ATGAGGAGAG CATAAGGAAG CCCAGCCCAG AGCCACGAGC TCAGATGAGG
    AGGGCTGCTA GCAGAGATCA ACTTAGGGAC AGTAGTCCAC CCCCCGCATT CAAGCCAGAG
    CCGCCCAAGG CCAAAACTCA GAACAGAGAA GAATCCTTTG ACATCTCCAG ATCCCATGAC
    TATAAGTCAA ACCCCTCAGC CGTTGCTGGT AATGAAGTTT CTGGGGCATC TACCAGAAGC
    TGTCCTCCTC CTATTGCAGC AAAACCTTCC TTTGGAAGGT CTATACTGAA GCCCTCCACT
    CCTGTCCCTT CCCCAGAGAG TGAGGAGGTG GGAGAGGGCA GTGAAGAGCA AGAAGGCGCT
    CCCAAGTCTG TCCTGGGCAA AGTTAAAATA TTTGAGAAGA TGGATCACAA GGCAAGATTA
    CAGAGAATGC AGGAGCTTCA AGAAGCACAG AATGCAAGGA TTGAAATTGC TCAGAAGCAT
    CCTGACATCT ATGCAGTTCC AATCAAAACA CACAAGCCAG ACCCTGGCCT GTCCCAGCAC
    ACAAGTTCCA GGCCACCCGA GCCCCAGAAA GGCCCTTCCA GGCTCTACCA GGATCCCAGA
    GGAAGTTATG GCAGTGATGC TGAAGAGGAA GAATACCGCC AGCAGCTGTC CGAACACTCG
    AAGCGTGGCT ATTACAGCCA GCCTTCCCGG TACCGGGACA CGGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01215
    Clone ID Related Accession (Same CDS sequence) XM_005615721.2 , XM_005615721.3
    Accession Version XM_005615721.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tight junction protein ZO-2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA GATCAACTTA GGGACAGTAG TCCACCCCCC
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACTCAGAACA GAGAAGAATC CTTTGACATC
    TCCAGATCCC ATGACTATAA GTCAAACCCC TCAGCCGTTG CTGGTAATGA AGTTTCTGGG
    GCATCTACCA GAAGCTGTCC TCCTCCTATT GCAGCAAAAC CTTCCTTTGG AAGGTCTATA
    CTGAAGCCCT CCACTCCTGT CCCTTCCCCA GAGAGTGAGG AGGTGGGAGA GGGCAGTGAA
    GAGCAAGAAG GCGCTCCCAA GTCTGTCCTG GGCAAAGTTA AAATATTTGA GAAGATGGAT
    CACAAGGCAA GATTACAGAG AATGCAGGAG CTTCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA CATCTATGCA GTTCCAATCA AAACACACAA GCCAGACCCT
    GGCCTGTCCC AGCACACAAG TTCCAGGCCA CCCGAGCCCC AGAAAGGCCC TTCCAGGCTC
    TACCAGGATC CCAGAGGAAG TTATGGCAGT GATGCTGAAG AGGAAGAATA CCGCCAGCAG
    CTGTCCGAAC ACTCGAAGCG TGGCTATTAC AGCCAGCCTT CCCGGTACCG GGACACGGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615778.1
    CDS45..3650
    Translation

    Target ORF information:

    RefSeq Version XM_005615721.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615721.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA GATCAACTTA GGGACAGTAG TCCACCCCCC
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACTCAGAACA GAGAAGAATC CTTTGACATC
    TCCAGATCCC ATGACTATAA GTCAAACCCC TCAGCCGTTG CTGGTAATGA AGTTTCTGGG
    GCATCTACCA GAAGCTGTCC TCCTCCTATT GCAGCAAAAC CTTCCTTTGG AAGGTCTATA
    CTGAAGCCCT CCACTCCTGT CCCTTCCCCA GAGAGTGAGG AGGTGGGAGA GGGCAGTGAA
    GAGCAAGAAG GCGCTCCCAA GTCTGTCCTG GGCAAAGTTA AAATATTTGA GAAGATGGAT
    CACAAGGCAA GATTACAGAG AATGCAGGAG CTTCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA CATCTATGCA GTTCCAATCA AAACACACAA GCCAGACCCT
    GGCCTGTCCC AGCACACAAG TTCCAGGCCA CCCGAGCCCC AGAAAGGCCC TTCCAGGCTC
    TACCAGGATC CCAGAGGAAG TTATGGCAGT GATGCTGAAG AGGAAGAATA CCGCCAGCAG
    CTGTCCGAAC ACTCGAAGCG TGGCTATTAC AGCCAGCCTT CCCGGTACCG GGACACGGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01215
    Clone ID Related Accession (Same CDS sequence) XM_005615721.2 , XM_005615721.3
    Accession Version XM_005615721.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA GATCAACTTA GGGACAGTAG TCCACCCCCC
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACTCAGAACA GAGAAGAATC CTTTGACATC
    TCCAGATCCC ATGACTATAA GTCAAACCCC TCAGCCGTTG CTGGTAATGA AGTTTCTGGG
    GCATCTACCA GAAGCTGTCC TCCTCCTATT GCAGCAAAAC CTTCCTTTGG AAGGTCTATA
    CTGAAGCCCT CCACTCCTGT CCCTTCCCCA GAGAGTGAGG AGGTGGGAGA GGGCAGTGAA
    GAGCAAGAAG GCGCTCCCAA GTCTGTCCTG GGCAAAGTTA AAATATTTGA GAAGATGGAT
    CACAAGGCAA GATTACAGAG AATGCAGGAG CTTCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA CATCTATGCA GTTCCAATCA AAACACACAA GCCAGACCCT
    GGCCTGTCCC AGCACACAAG TTCCAGGCCA CCCGAGCCCC AGAAAGGCCC TTCCAGGCTC
    TACCAGGATC CCAGAGGAAG TTATGGCAGT GATGCTGAAG AGGAAGAATA CCGCCAGCAG
    CTGTCCGAAC ACTCGAAGCG TGGCTATTAC AGCCAGCCTT CCCGGTACCG GGACACGGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615778.1
    CDS70..3675
    Translation

    Target ORF information:

    RefSeq Version XM_005615721.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615721.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA GATCAACTTA GGGACAGTAG TCCACCCCCC
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACTCAGAACA GAGAAGAATC CTTTGACATC
    TCCAGATCCC ATGACTATAA GTCAAACCCC TCAGCCGTTG CTGGTAATGA AGTTTCTGGG
    GCATCTACCA GAAGCTGTCC TCCTCCTATT GCAGCAAAAC CTTCCTTTGG AAGGTCTATA
    CTGAAGCCCT CCACTCCTGT CCCTTCCCCA GAGAGTGAGG AGGTGGGAGA GGGCAGTGAA
    GAGCAAGAAG GCGCTCCCAA GTCTGTCCTG GGCAAAGTTA AAATATTTGA GAAGATGGAT
    CACAAGGCAA GATTACAGAG AATGCAGGAG CTTCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA CATCTATGCA GTTCCAATCA AAACACACAA GCCAGACCCT
    GGCCTGTCCC AGCACACAAG TTCCAGGCCA CCCGAGCCCC AGAAAGGCCC TTCCAGGCTC
    TACCAGGATC CCAGAGGAAG TTATGGCAGT GATGCTGAAG AGGAAGAATA CCGCCAGCAG
    CTGTCCGAAC ACTCGAAGCG TGGCTATTAC AGCCAGCCTT CCCGGTACCG GGACACGGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01216
    Clone ID Related Accession (Same CDS sequence) XM_005615722.2
    Accession Version XM_005615722.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GAGGAGATCG CGGGTTCCCA CCCGGGCGGG AGCTGTCAGG CTGGCTCCGC 
    GCCCCAGGCA TGGAAGAACT GATATGGGAG CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TCGTCATTTC TGACGTGCTA CCGGGTGGGC CTGCTGATGG GCTGCTTCAA
    GAAAATGACA GGGTGGTCAT GGTTAACGGC ACCCCCATGG AGGATGTGCT CCATTCATTT
    GCTGTCCAGC AGCTGAGAAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCC CCAGGGCAGC CTACCCGTCG ATGAGGATGA CCGTGCTTTT
    GAGGTGATGG ATGAGTTCGA TGGCAGAAGT GCCCGCAGCG GGTACAGCGA GAGGAGCCGG
    CGCAGCAGCC ACGGTGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAGAG GGGGCGTCCC
    CATGAGCGGG CGTGGAGCCA GGAGCGCGAG CGCAGCCGTG GCCGGAGCCT GGAGCGGGGC
    CTGGACCATG ACGATGACTA CCGGCGGCCT CGAGAGCGCA GCCGGGGCCG GAGCCTGGAG
    CGGGGCCTGG ACCATGACGA TGACTACGGG CGGCCCGGAG AGCGCAGCCA CGGCATGAGC
    ACTGACAGGG GCTACGACCG TGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGCACC
    TATAGCCCAG AGGCCGAGTA TGGCCGCAGG GCCCAGCCTG ATGCCCGGCA CGCAGGGTCC
    CGGAGCCGCA GCCGCGAGCA CTTGCGCTCC CGCAGCCCCA GCCCTGAGCT GAGGGGGCGG
    CCGGACCATG CAGGCCAGCC TGACTCGGAC AGGCCCATCG GGGTCCTTCT GATGAAAAGC
    AAAGCAAATG AAGAGTACGG TCTCCGGCTT GGGAGTCAGA TCTTCATAAA GGAAATGACC
    AGAACCGGTC TGGCAACTAA AGATGGCAAC CTGCACGAAG GAGACATAAT TCTCAAGATA
    AATGGAACTG TAACTGAGAA CATGTCTTTA ACTGATGCCC GAAAACTGAT AGAAAAGTCA
    AGAGGAAAAC TCCAACTAGT GGTGTTGAGA GACAGCAAGC AAACGCTCAT CAACATTCCA
    TCATTAAATG ACAGCGACTC TGAAATAGAA GATATCTCAG AAATAGAGTC AAACCGATCA
    TTTTCTCCAG AGGAGAGACG GCAGCAGTAT TCCGACTATG ATTATCATTC CTCAAATGAA
    AAACTGAAGG AAAGGCCAAA TTCAAGAGAG GACATGCAGA ACAGATGGTC CAGGATGGGC
    GCCACACCCA CTCCCTTTAA GTCCATGGGG GATATTGCAT CTGTGGTTGG CACAGAGAAC
    AGCAAGGAAC CCAGATACCA AGAGGAACCA CCAGCTCCTC AACCTAAAGC AGCCCCAAGA
    ACTTTTCTTC GCCCGAGTCC TGAAGATGAA GCAATATATG GTCCCAATAC CAAAATGGTG
    AGGTTTAAGA AGGGAGACAG CGTGGGACTC CGATTGGCTG GTGGCAATGA CGTTGGGATA
    TTTGTGGCTG GTATTCAAGA GGGTACCTCG GCAGAACAAG AAGGCCTTCA AGAGGGGGAC
    CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TTCGGGAAGA TGCCGTTCTC
    TACCTGCTAG AAATCCCTAA AGGTGAAATG GTGACCATTT TAGCTCAGAG CCGAGCTGAT
    GTGTATAGAG ACATCCTGGC TTGTGGCAGA GGGGACTCAT TTTTTATAAG AAGCCACTTT
    GAATGTGAGA AGGAAACTCC ACAGAGCCTG GCCTTCAGCA GGGGGGAGGT CTTCCGAGTT
    GTAGATACAC TGTATGATGG CAAGCTGGGC CACTGGCTGG CTGTGAGGAT TGGAAACGAA
    TTGGAGAAGG GCTTAATCCC CAACAAAAGC AGAGCTGAAC AAATGGCCAG TGTTCAGAAT
    GCCCAGAGAG ACAATGCTGG GGACAGAGCA GATTTCTGGA GAATGCGTGG CCAGAGATCT
    GGCATGAAGA AGAACTTAAG GAAAAGTCGG GAAGATCTAA CAGCTGCTGT GTCAGTTAGC
    ACCAAGTTCC CAGCTTACGA AAGGGTTTTG CTGCGAGAAG CTGGTTTCAA GAGACCTGTG
    GTCTTATTTG GCCCTATAGC AGATATAGCC TTGGAAAAGT TGGCAAATGA GTTACCGGAC
    CTGTTCCAAA CTGCTAAAAC GGAACCAAAA GATGCAGGAT CCGAGAAATC TAGTGGGGTG
    GTCCGGTTAA ATACTGTGAG GCAAATTATT GAACAGGATA AGCATGCACT ATTGGATGTG
    ACTCCTAAAG CTGTGGACCT GTTGAATTAT ACTCAGTGGT TCCCGATTGT GATTTTTTTC
    AACCCAGACT CTAGACAAGG TGTCAAAACC ATGAGACAGA GGTTGAATCC AACATCCAAC
    AAAAGTTCTC GGAAGTTGTA TGATCAAGCC AACAAGCTTA AGAAAACGTG TGCACATCTT
    TTTACAGCTA CTATCAACCT AAATTCAGCC AATGATAGCT GGTTTGGCAG CTTGAAGGAC
    ACAATTCAGC ATCAGCAAGG AGAAGCAGTT TGGGTCTCTG AAGGAAAGAT GGAAGGGATG
    GATGATGACC CTGAAGACCG CATGTCCTAC TTAACTGCCA TGGGCGCAGA CTATCTGAGT
    TGCGACAGCC GCCTCATCAG TGACTTTGAA GACACCGACG GTGAAGGAGG TGCCTACACT
    GACAATGAGC TGGATGAGCC AGCTGAGGAG CCACTGGTGT CTTCCATCAC CCGCTCCTCG
    GAGCCGGTGC AGCATGAGGA GAGCATAAGG AAGCCCAGCC CAGAGCCACG AGCTCAGATG
    AGGAGGGCTG CTAGCAGAGA TCAACTTAGG GACAGTAGTC CACCCCCCGC ATTCAAGCCA
    GAGCCGCCCA AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GGTAATGAAG TTTCTGGGGC ATCTACCAGA
    AGCTGTCCTC CTCCTATTGC AGCAAAACCT TCCTTTGGAA GGTCTATACT GAAGCCCTCC
    ACTCCTGTCC CTTCCCCAGA GAGTGAGGAG GTGGGAGAGG GCAGTGAAGA GCAAGAAGGC
    GCTCCCAAGT CTGTCCTGGG CAAAGTTAAA ATATTTGAGA AGATGGATCA CAAGGCAAGA
    TTACAGAGAA TGCAGGAGCT TCAAGAAGCA CAGAATGCAA GGATTGAAAT TGCTCAGAAG
    CATCCTGACA TCTATGCAGT TCCAATCAAA ACACACAAGC CAGACCCTGG CCTGTCCCAG
    CACACAAGTT CCAGGCCACC CGAGCCCCAG AAAGGCCCTT CCAGGCTCTA CCAGGATCCC
    AGAGGAAGTT ATGGCAGTGA TGCTGAAGAG GAAGAATACC GCCAGCAGCT GTCCGAACAC
    TCGAAGCGTG GCTATTACAG CCAGCCTTCC CGGTACCGGG ACACGGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615779.1
    CDS76..3669
    Translation

    Target ORF information:

    RefSeq Version XM_005615722.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615722.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GAGGAGATCG CGGGTTCCCA CCCGGGCGGG AGCTGTCAGG CTGGCTCCGC 
    GCCCCAGGCA TGGAAGAACT GATATGGGAG CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TCGTCATTTC TGACGTGCTA CCGGGTGGGC CTGCTGATGG GCTGCTTCAA
    GAAAATGACA GGGTGGTCAT GGTTAACGGC ACCCCCATGG AGGATGTGCT CCATTCATTT
    GCTGTCCAGC AGCTGAGAAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCC CCAGGGCAGC CTACCCGTCG ATGAGGATGA CCGTGCTTTT
    GAGGTGATGG ATGAGTTCGA TGGCAGAAGT GCCCGCAGCG GGTACAGCGA GAGGAGCCGG
    CGCAGCAGCC ACGGTGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAGAG GGGGCGTCCC
    CATGAGCGGG CGTGGAGCCA GGAGCGCGAG CGCAGCCGTG GCCGGAGCCT GGAGCGGGGC
    CTGGACCATG ACGATGACTA CCGGCGGCCT CGAGAGCGCA GCCGGGGCCG GAGCCTGGAG
    CGGGGCCTGG ACCATGACGA TGACTACGGG CGGCCCGGAG AGCGCAGCCA CGGCATGAGC
    ACTGACAGGG GCTACGACCG TGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGCACC
    TATAGCCCAG AGGCCGAGTA TGGCCGCAGG GCCCAGCCTG ATGCCCGGCA CGCAGGGTCC
    CGGAGCCGCA GCCGCGAGCA CTTGCGCTCC CGCAGCCCCA GCCCTGAGCT GAGGGGGCGG
    CCGGACCATG CAGGCCAGCC TGACTCGGAC AGGCCCATCG GGGTCCTTCT GATGAAAAGC
    AAAGCAAATG AAGAGTACGG TCTCCGGCTT GGGAGTCAGA TCTTCATAAA GGAAATGACC
    AGAACCGGTC TGGCAACTAA AGATGGCAAC CTGCACGAAG GAGACATAAT TCTCAAGATA
    AATGGAACTG TAACTGAGAA CATGTCTTTA ACTGATGCCC GAAAACTGAT AGAAAAGTCA
    AGAGGAAAAC TCCAACTAGT GGTGTTGAGA GACAGCAAGC AAACGCTCAT CAACATTCCA
    TCATTAAATG ACAGCGACTC TGAAATAGAA GATATCTCAG AAATAGAGTC AAACCGATCA
    TTTTCTCCAG AGGAGAGACG GCAGCAGTAT TCCGACTATG ATTATCATTC CTCAAATGAA
    AAACTGAAGG AAAGGCCAAA TTCAAGAGAG GACATGCAGA ACAGATGGTC CAGGATGGGC
    GCCACACCCA CTCCCTTTAA GTCCATGGGG GATATTGCAT CTGTGGTTGG CACAGAGAAC
    AGCAAGGAAC CCAGATACCA AGAGGAACCA CCAGCTCCTC AACCTAAAGC AGCCCCAAGA
    ACTTTTCTTC GCCCGAGTCC TGAAGATGAA GCAATATATG GTCCCAATAC CAAAATGGTG
    AGGTTTAAGA AGGGAGACAG CGTGGGACTC CGATTGGCTG GTGGCAATGA CGTTGGGATA
    TTTGTGGCTG GTATTCAAGA GGGTACCTCG GCAGAACAAG AAGGCCTTCA AGAGGGGGAC
    CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TTCGGGAAGA TGCCGTTCTC
    TACCTGCTAG AAATCCCTAA AGGTGAAATG GTGACCATTT TAGCTCAGAG CCGAGCTGAT
    GTGTATAGAG ACATCCTGGC TTGTGGCAGA GGGGACTCAT TTTTTATAAG AAGCCACTTT
    GAATGTGAGA AGGAAACTCC ACAGAGCCTG GCCTTCAGCA GGGGGGAGGT CTTCCGAGTT
    GTAGATACAC TGTATGATGG CAAGCTGGGC CACTGGCTGG CTGTGAGGAT TGGAAACGAA
    TTGGAGAAGG GCTTAATCCC CAACAAAAGC AGAGCTGAAC AAATGGCCAG TGTTCAGAAT
    GCCCAGAGAG ACAATGCTGG GGACAGAGCA GATTTCTGGA GAATGCGTGG CCAGAGATCT
    GGCATGAAGA AGAACTTAAG GAAAAGTCGG GAAGATCTAA CAGCTGCTGT GTCAGTTAGC
    ACCAAGTTCC CAGCTTACGA AAGGGTTTTG CTGCGAGAAG CTGGTTTCAA GAGACCTGTG
    GTCTTATTTG GCCCTATAGC AGATATAGCC TTGGAAAAGT TGGCAAATGA GTTACCGGAC
    CTGTTCCAAA CTGCTAAAAC GGAACCAAAA GATGCAGGAT CCGAGAAATC TAGTGGGGTG
    GTCCGGTTAA ATACTGTGAG GCAAATTATT GAACAGGATA AGCATGCACT ATTGGATGTG
    ACTCCTAAAG CTGTGGACCT GTTGAATTAT ACTCAGTGGT TCCCGATTGT GATTTTTTTC
    AACCCAGACT CTAGACAAGG TGTCAAAACC ATGAGACAGA GGTTGAATCC AACATCCAAC
    AAAAGTTCTC GGAAGTTGTA TGATCAAGCC AACAAGCTTA AGAAAACGTG TGCACATCTT
    TTTACAGCTA CTATCAACCT AAATTCAGCC AATGATAGCT GGTTTGGCAG CTTGAAGGAC
    ACAATTCAGC ATCAGCAAGG AGAAGCAGTT TGGGTCTCTG AAGGAAAGAT GGAAGGGATG
    GATGATGACC CTGAAGACCG CATGTCCTAC TTAACTGCCA TGGGCGCAGA CTATCTGAGT
    TGCGACAGCC GCCTCATCAG TGACTTTGAA GACACCGACG GTGAAGGAGG TGCCTACACT
    GACAATGAGC TGGATGAGCC AGCTGAGGAG CCACTGGTGT CTTCCATCAC CCGCTCCTCG
    GAGCCGGTGC AGCATGAGGA GAGCATAAGG AAGCCCAGCC CAGAGCCACG AGCTCAGATG
    AGGAGGGCTG CTAGCAGAGA TCAACTTAGG GACAGTAGTC CACCCCCCGC ATTCAAGCCA
    GAGCCGCCCA AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GGTAATGAAG TTTCTGGGGC ATCTACCAGA
    AGCTGTCCTC CTCCTATTGC AGCAAAACCT TCCTTTGGAA GGTCTATACT GAAGCCCTCC
    ACTCCTGTCC CTTCCCCAGA GAGTGAGGAG GTGGGAGAGG GCAGTGAAGA GCAAGAAGGC
    GCTCCCAAGT CTGTCCTGGG CAAAGTTAAA ATATTTGAGA AGATGGATCA CAAGGCAAGA
    TTACAGAGAA TGCAGGAGCT TCAAGAAGCA CAGAATGCAA GGATTGAAAT TGCTCAGAAG
    CATCCTGACA TCTATGCAGT TCCAATCAAA ACACACAAGC CAGACCCTGG CCTGTCCCAG
    CACACAAGTT CCAGGCCACC CGAGCCCCAG AAAGGCCCTT CCAGGCTCTA CCAGGATCCC
    AGAGGAAGTT ATGGCAGTGA TGCTGAAGAG GAAGAATACC GCCAGCAGCT GTCCGAACAC
    TCGAAGCGTG GCTATTACAG CCAGCCTTCC CGGTACCGGG ACACGGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01217
    Clone ID Related Accession (Same CDS sequence) XM_005615725.2 , XM_005615725.3 , XM_022416196.1
    Accession Version XM_005615725.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tight junction protein ZO-2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615782.1
    CDS269..3793
    Translation

    Target ORF information:

    RefSeq Version XM_005615725.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615725.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01217
    Clone ID Related Accession (Same CDS sequence) XM_005615725.2 , XM_005615725.3 , XM_022416196.1
    Accession Version XM_005615725.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615782.1
    CDS287..3811
    Translation

    Target ORF information:

    RefSeq Version XM_005615725.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615725.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01217
    Clone ID Related Accession (Same CDS sequence) XM_005615725.2 , XM_005615725.3 , XM_022416196.1
    Accession Version XM_022416196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271904.1
    CDS92..3616
    Translation

    Target ORF information:

    RefSeq Version XM_022416196.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGGTAATGAA GTTTCTGGGG CATCTACCAG AAGCTGTCCT
    CCTCCTATTG CAGCAAAACC TTCCTTTGGA AGGTCTATAC TGAAGCCCTC CACTCCTGTC
    CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG
    TCTGTCCTGG GCAAAGTTAA AATATTTGAG AAGATGGATC ACAAGGCAAG ATTACAGAGA
    ATGCAGGAGC TTCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAC
    ATCTATGCAG TTCCAATCAA AACACACAAG CCAGACCCTG GCCTGTCCCA GCACACAAGT
    TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT TCCAGGCTCT ACCAGGATCC CAGAGGAAGT
    TATGGCAGTG ATGCTGAAGA GGAAGAATAC CGCCAGCAGC TGTCCGAACA CTCGAAGCGT
    GGCTATTACA GCCAGCCTTC CCGGTACCGG GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01218
    Clone ID Related Accession (Same CDS sequence) XM_005615724.2 , XM_005615724.3
    Accession Version XM_005615724.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tight junction protein ZO-2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGGCCAAAA CTCAGAACAG AGAAGAATCC
    TTTGACATCT CCAGATCCCA TGACTATAAG TCAAACCCCT CAGCCGTTGC TGGTAATGAA
    GTTTCTGGGG CATCTACCAG AAGCTGTCCT CCTCCTATTG CAGCAAAACC TTCCTTTGGA
    AGGTCTATAC TGAAGCCCTC CACTCCTGTC CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG
    GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG TCTGTCCTGG GCAAAGTTAA AATATTTGAG
    AAGATGGATC ACAAGGCAAG ATTACAGAGA ATGCAGGAGC TTCAAGAAGC ACAGAATGCA
    AGGATTGAAA TTGCTCAGAA GCATCCTGAC ATCTATGCAG TTCCAATCAA AACACACAAG
    CCAGACCCTG GCCTGTCCCA GCACACAAGT TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT
    TCCAGGCTCT ACCAGGATCC CAGAGGAAGT TATGGCAGTG ATGCTGAAGA GGAAGAATAC
    CGCCAGCAGC TGTCCGAACA CTCGAAGCGT GGCTATTACA GCCAGCCTTC CCGGTACCGG
    GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615781.1
    CDS46..3540
    Translation

    Target ORF information:

    RefSeq Version XM_005615724.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615724.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGGCCAAAA CTCAGAACAG AGAAGAATCC
    TTTGACATCT CCAGATCCCA TGACTATAAG TCAAACCCCT CAGCCGTTGC TGGTAATGAA
    GTTTCTGGGG CATCTACCAG AAGCTGTCCT CCTCCTATTG CAGCAAAACC TTCCTTTGGA
    AGGTCTATAC TGAAGCCCTC CACTCCTGTC CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG
    GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG TCTGTCCTGG GCAAAGTTAA AATATTTGAG
    AAGATGGATC ACAAGGCAAG ATTACAGAGA ATGCAGGAGC TTCAAGAAGC ACAGAATGCA
    AGGATTGAAA TTGCTCAGAA GCATCCTGAC ATCTATGCAG TTCCAATCAA AACACACAAG
    CCAGACCCTG GCCTGTCCCA GCACACAAGT TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT
    TCCAGGCTCT ACCAGGATCC CAGAGGAAGT TATGGCAGTG ATGCTGAAGA GGAAGAATAC
    CGCCAGCAGC TGTCCGAACA CTCGAAGCGT GGCTATTACA GCCAGCCTTC CCGGTACCGG
    GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01218
    Clone ID Related Accession (Same CDS sequence) XM_005615724.2 , XM_005615724.3
    Accession Version XM_005615724.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGGCCAAAA CTCAGAACAG AGAAGAATCC
    TTTGACATCT CCAGATCCCA TGACTATAAG TCAAACCCCT CAGCCGTTGC TGGTAATGAA
    GTTTCTGGGG CATCTACCAG AAGCTGTCCT CCTCCTATTG CAGCAAAACC TTCCTTTGGA
    AGGTCTATAC TGAAGCCCTC CACTCCTGTC CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG
    GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG TCTGTCCTGG GCAAAGTTAA AATATTTGAG
    AAGATGGATC ACAAGGCAAG ATTACAGAGA ATGCAGGAGC TTCAAGAAGC ACAGAATGCA
    AGGATTGAAA TTGCTCAGAA GCATCCTGAC ATCTATGCAG TTCCAATCAA AACACACAAG
    CCAGACCCTG GCCTGTCCCA GCACACAAGT TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT
    TCCAGGCTCT ACCAGGATCC CAGAGGAAGT TATGGCAGTG ATGCTGAAGA GGAAGAATAC
    CGCCAGCAGC TGTCCGAACA CTCGAAGCGT GGCTATTACA GCCAGCCTTC CCGGTACCGG
    GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615781.1
    CDS71..3565
    Translation

    Target ORF information:

    RefSeq Version XM_005615724.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615724.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACTG CTCAGGTCCT CCAGAGGATG TGGATCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCCCCAGG CATGGAAGAA CTGATATGGG AGCAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATCGTCATT TCTGACGTGC TACCGGGTGG GCCTGCTGAT
    GGGCTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTTAACG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCTGTCCA GCAGCTGAGA AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CCCCAGGGCA GCCTACCCGT CGATGAGGAT
    GACCGTGCTT TTGAGGTGAT GGATGAGTTC GATGGCAGAA GTGCCCGCAG CGGGTACAGC
    GAGAGGAGCC GGCGCAGCAG CCACGGTGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAG
    AGGGGGCGTC CCCATGAGCG GGCGTGGAGC CAGGAGCGCG AGCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA TGACGATGAC TACCGGCGGC CTCGAGAGCG CAGCCGGGGC
    CGGAGCCTGG AGCGGGGCCT GGACCATGAC GATGACTACG GGCGGCCCGG AGAGCGCAGC
    CACGGCATGA GCACTGACAG GGGCTACGAC CGTGGCTACG ACCGTGGCTA CGACCGTGGC
    TACGACCGCA CCTATAGCCC AGAGGCCGAG TATGGCCGCA GGGCCCAGCC TGATGCCCGG
    CACGCAGGGT CCCGGAGCCG CAGCCGCGAG CACTTGCGCT CCCGCAGCCC CAGCCCTGAG
    CTGAGGGGGC GGCCGGACCA TGCAGGCCAG CCTGACTCGG ACAGGCCCAT CGGGGTCCTT
    CTGATGAAAA GCAAAGCAAA TGAAGAGTAC GGTCTCCGGC TTGGGAGTCA GATCTTCATA
    AAGGAAATGA CCAGAACCGG TCTGGCAACT AAAGATGGCA ACCTGCACGA AGGAGACATA
    ATTCTCAAGA TAAATGGAAC TGTAACTGAG AACATGTCTT TAACTGATGC CCGAAAACTG
    ATAGAAAAGT CAAGAGGAAA ACTCCAACTA GTGGTGTTGA GAGACAGCAA GCAAACGCTC
    ATCAACATTC CATCATTAAA TGACAGCGAC TCTGAAATAG AAGATATCTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGGCAGCAGT ATTCCGACTA TGATTATCAT
    TCCTCAAATG AAAAACTGAA GGAAAGGCCA AATTCAAGAG AGGACATGCA GAACAGATGG
    TCCAGGATGG GCGCCACACC CACTCCCTTT AAGTCCATGG GGGATATTGC ATCTGTGGTT
    GGCACAGAGA ACAGCAAGGA ACCCAGATAC CAAGAGGAAC CACCAGCTCC TCAACCTAAA
    GCAGCCCCAA GAACTTTTCT TCGCCCGAGT CCTGAAGATG AAGCAATATA TGGTCCCAAT
    ACCAAAATGG TGAGGTTTAA GAAGGGAGAC AGCGTGGGAC TCCGATTGGC TGGTGGCAAT
    GACGTTGGGA TATTTGTGGC TGGTATTCAA GAGGGTACCT CGGCAGAACA AGAAGGCCTT
    CAAGAGGGGG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGGCT AGTTCGGGAA
    GATGCCGTTC TCTACCTGCT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCTG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGACTC ATTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAG CAGGGGGGAG
    GTCTTCCGAG TTGTAGATAC ACTGTATGAT GGCAAGCTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAACG AATTGGAGAA GGGCTTAATC CCCAACAAAA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAGA ATGCCCAGAG AGACAATGCT GGGGACAGAG CAGATTTCTG GAGAATGCGT
    GGCCAGAGAT CTGGCATGAA GAAGAACTTA AGGAAAAGTC GGGAAGATCT AACAGCTGCT
    GTGTCAGTTA GCACCAAGTT CCCAGCTTAC GAAAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT TGGCCCTATA GCAGATATAG CCTTGGAAAA GTTGGCAAAT
    GAGTTACCGG ACCTGTTCCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCCGAGAAA
    TCTAGTGGGG TGGTCCGGTT AAATACTGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTATTGGATG TGACTCCTAA AGCTGTGGAC CTGTTGAATT ATACTCAGTG GTTCCCGATT
    GTGATTTTTT TCAACCCAGA CTCTAGACAA GGTGTCAAAA CCATGAGACA GAGGTTGAAT
    CCAACATCCA ACAAAAGTTC TCGGAAGTTG TATGATCAAG CCAACAAGCT TAAGAAAACG
    TGTGCACATC TTTTTACAGC TACTATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTGAAGG ACACAATTCA GCATCAGCAA GGAGAAGCAG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCTGAAGAC CGCATGTCCT ACTTAACTGC CATGGGCGCA
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACCGA CGGTGAAGGA
    GGTGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCACTGGT GTCTTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCATGAG GAGGCCAAAA CTCAGAACAG AGAAGAATCC
    TTTGACATCT CCAGATCCCA TGACTATAAG TCAAACCCCT CAGCCGTTGC TGGTAATGAA
    GTTTCTGGGG CATCTACCAG AAGCTGTCCT CCTCCTATTG CAGCAAAACC TTCCTTTGGA
    AGGTCTATAC TGAAGCCCTC CACTCCTGTC CCTTCCCCAG AGAGTGAGGA GGTGGGAGAG
    GGCAGTGAAG AGCAAGAAGG CGCTCCCAAG TCTGTCCTGG GCAAAGTTAA AATATTTGAG
    AAGATGGATC ACAAGGCAAG ATTACAGAGA ATGCAGGAGC TTCAAGAAGC ACAGAATGCA
    AGGATTGAAA TTGCTCAGAA GCATCCTGAC ATCTATGCAG TTCCAATCAA AACACACAAG
    CCAGACCCTG GCCTGTCCCA GCACACAAGT TCCAGGCCAC CCGAGCCCCA GAAAGGCCCT
    TCCAGGCTCT ACCAGGATCC CAGAGGAAGT TATGGCAGTG ATGCTGAAGA GGAAGAATAC
    CGCCAGCAGC TGTCCGAACA CTCGAAGCGT GGCTATTACA GCCAGCCTTC CCGGTACCGG
    GACACGGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285678
    Clone ID Related Accession (Same CDS sequence) XM_022415947.1
    Accession Version XM_022415947.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GAGAGTGAGG AGGTGGGAGA GGGCAGTGAA
    GAGCAAGAAG GCGCTCCCAA GTCTGTCCTG GGCAAAGTTA AAATATTTGA GAAGATGGAT
    CACAAGGCAA GATTACAGAG AATGCAGGAG CTTCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA CATCTATGCA GTTCCAATCA AAACACACAA GCCAGACCCT
    GGCCTGTCCC AGCACACAAG TTCCAGGCCA CCCGAGCCCC AGAAAGGCCC TTCCAGGCTC
    TACCAGGATC CCAGAGGAAG TTATGGCAGT GATGCTGAAG AGGAAGAATA CCGCCAGCAG
    CTGTCCGAAC ACTCGAAGCG TGGCTATTAC AGCCAGCCTT CCCGGTACCG GGACACGGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271655.1
    CDS131..3436
    Translation

    Target ORF information:

    RefSeq Version XM_022415947.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GAGAGTGAGG AGGTGGGAGA GGGCAGTGAA
    GAGCAAGAAG GCGCTCCCAA GTCTGTCCTG GGCAAAGTTA AAATATTTGA GAAGATGGAT
    CACAAGGCAA GATTACAGAG AATGCAGGAG CTTCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA CATCTATGCA GTTCCAATCA AAACACACAA GCCAGACCCT
    GGCCTGTCCC AGCACACAAG TTCCAGGCCA CCCGAGCCCC AGAAAGGCCC TTCCAGGCTC
    TACCAGGATC CCAGAGGAAG TTATGGCAGT GATGCTGAAG AGGAAGAATA CCGCCAGCAG
    CTGTCCGAAC ACTCGAAGCG TGGCTATTAC AGCCAGCCTT CCCGGTACCG GGACACGGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34545
    Clone ID Related Accession (Same CDS sequence) XM_022414943.1 , XM_005615719.2
    Accession Version XM_022414943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGAGAGTGAG GAGGTGGGAG AGGGCAGTGA AGAGCAAGAA
    GGCGCTCCCA AGTCTGTCCT GGGCAAAGTT AAAATATTTG AGAAGATGGA TCACAAGGCA
    AGATTACAGA GAATGCAGGA GCTTCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ACATCTATGC AGTTCCAATC AAAACACACA AGCCAGACCC TGGCCTGTCC
    CAGCACACAA GTTCCAGGCC ACCCGAGCCC CAGAAAGGCC CTTCCAGGCT CTACCAGGAT
    CCCAGAGGAA GTTATGGCAG TGATGCTGAA GAGGAAGAAT ACCGCCAGCA GCTGTCCGAA
    CACTCGAAGC GTGGCTATTA CAGCCAGCCT TCCCGGTACC GGGACACGGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270651.1
    CDS131..3547
    Translation

    Target ORF information:

    RefSeq Version XM_022414943.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGAGAGTGAG GAGGTGGGAG AGGGCAGTGA AGAGCAAGAA
    GGCGCTCCCA AGTCTGTCCT GGGCAAAGTT AAAATATTTG AGAAGATGGA TCACAAGGCA
    AGATTACAGA GAATGCAGGA GCTTCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ACATCTATGC AGTTCCAATC AAAACACACA AGCCAGACCC TGGCCTGTCC
    CAGCACACAA GTTCCAGGCC ACCCGAGCCC CAGAAAGGCC CTTCCAGGCT CTACCAGGAT
    CCCAGAGGAA GTTATGGCAG TGATGCTGAA GAGGAAGAAT ACCGCCAGCA GCTGTCCGAA
    CACTCGAAGC GTGGCTATTA CAGCCAGCCT TCCCGGTACC GGGACACGGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34545
    Clone ID Related Accession (Same CDS sequence) XM_022414943.1 , XM_005615719.2
    Accession Version XM_005615719.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tight junction protein ZO-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGAGAGTGAG GAGGTGGGAG AGGGCAGTGA AGAGCAAGAA
    GGCGCTCCCA AGTCTGTCCT GGGCAAAGTT AAAATATTTG AGAAGATGGA TCACAAGGCA
    AGATTACAGA GAATGCAGGA GCTTCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ACATCTATGC AGTTCCAATC AAAACACACA AGCCAGACCC TGGCCTGTCC
    CAGCACACAA GTTCCAGGCC ACCCGAGCCC CAGAAAGGCC CTTCCAGGCT CTACCAGGAT
    CCCAGAGGAA GTTATGGCAG TGATGCTGAA GAGGAAGAAT ACCGCCAGCA GCTGTCCGAA
    CACTCGAAGC GTGGCTATTA CAGCCAGCCT TCCCGGTACC GGGACACGGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615776.2
    CDS152..3568
    Translation

    Target ORF information:

    RefSeq Version XM_005615719.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615719.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAGCCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAGTAGT CCACCCCCCG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CTCAGAACAG AGAAGAATCC TTTGACATCT CCAGATCCCA TGACTATAAG
    TCAAACCCCT CAGCCGTTGC TGAGAGTGAG GAGGTGGGAG AGGGCAGTGA AGAGCAAGAA
    GGCGCTCCCA AGTCTGTCCT GGGCAAAGTT AAAATATTTG AGAAGATGGA TCACAAGGCA
    AGATTACAGA GAATGCAGGA GCTTCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ACATCTATGC AGTTCCAATC AAAACACACA AGCCAGACCC TGGCCTGTCC
    CAGCACACAA GTTCCAGGCC ACCCGAGCCC CAGAAAGGCC CTTCCAGGCT CTACCAGGAT
    CCCAGAGGAA GTTATGGCAG TGATGCTGAA GAGGAAGAAT ACCGCCAGCA GCTGTCCGAA
    CACTCGAAGC GTGGCTATTA CAGCCAGCCT TCCCGGTACC GGGACACGGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34546
    Clone ID Related Accession (Same CDS sequence) XM_005615720.2 , XM_022415227.1
    Accession Version XM_005615720.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tight junction protein ZO-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GGTAATGAAG TTTCTGGGGC ATCTACCAGA
    AGCTGTCCTC CTCCTATTGC AGCAAAACCT TCCTTTGGAA GGTCTATACT GAAGCCCTCC
    ACTCCTGTCC CTTCCCCAGA GAGTGAGGAG GTGGGAGAGG GCAGTGAAGA GCAAGAAGGC
    GCTCCCAAGT CTGTCCTGGG CAAAGTTAAA ATATTTGAGA AGATGGATCA CAAGGCAAGA
    TTACAGAGAA TGCAGGAGCT TCAAGAAGCA CAGAATGCAA GGATTGAAAT TGCTCAGAAG
    CATCCTGACA TCTATGCAGT TCCAATCAAA ACACACAAGC CAGACCCTGG CCTGTCCCAG
    CACACAAGTT CCAGGCCACC CGAGCCCCAG AAAGGCCCTT CCAGGCTCTA CCAGGATCCC
    AGAGGAAGTT ATGGCAGTGA TGCTGAAGAG GAAGAATACC GCCAGCAGCT GTCCGAACAC
    TCGAAGCGTG GCTATTACAG CCAGCCTTCC CGGTACCGGG ACACGGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615777.2
    CDS152..3565
    Translation

    Target ORF information:

    RefSeq Version XM_005615720.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615720.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GGTAATGAAG TTTCTGGGGC ATCTACCAGA
    AGCTGTCCTC CTCCTATTGC AGCAAAACCT TCCTTTGGAA GGTCTATACT GAAGCCCTCC
    ACTCCTGTCC CTTCCCCAGA GAGTGAGGAG GTGGGAGAGG GCAGTGAAGA GCAAGAAGGC
    GCTCCCAAGT CTGTCCTGGG CAAAGTTAAA ATATTTGAGA AGATGGATCA CAAGGCAAGA
    TTACAGAGAA TGCAGGAGCT TCAAGAAGCA CAGAATGCAA GGATTGAAAT TGCTCAGAAG
    CATCCTGACA TCTATGCAGT TCCAATCAAA ACACACAAGC CAGACCCTGG CCTGTCCCAG
    CACACAAGTT CCAGGCCACC CGAGCCCCAG AAAGGCCCTT CCAGGCTCTA CCAGGATCCC
    AGAGGAAGTT ATGGCAGTGA TGCTGAAGAG GAAGAATACC GCCAGCAGCT GTCCGAACAC
    TCGAAGCGTG GCTATTACAG CCAGCCTTCC CGGTACCGGG ACACGGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34546
    Clone ID Related Accession (Same CDS sequence) XM_005615720.2 , XM_022415227.1
    Accession Version XM_022415227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GGTAATGAAG TTTCTGGGGC ATCTACCAGA
    AGCTGTCCTC CTCCTATTGC AGCAAAACCT TCCTTTGGAA GGTCTATACT GAAGCCCTCC
    ACTCCTGTCC CTTCCCCAGA GAGTGAGGAG GTGGGAGAGG GCAGTGAAGA GCAAGAAGGC
    GCTCCCAAGT CTGTCCTGGG CAAAGTTAAA ATATTTGAGA AGATGGATCA CAAGGCAAGA
    TTACAGAGAA TGCAGGAGCT TCAAGAAGCA CAGAATGCAA GGATTGAAAT TGCTCAGAAG
    CATCCTGACA TCTATGCAGT TCCAATCAAA ACACACAAGC CAGACCCTGG CCTGTCCCAG
    CACACAAGTT CCAGGCCACC CGAGCCCCAG AAAGGCCCTT CCAGGCTCTA CCAGGATCCC
    AGAGGAAGTT ATGGCAGTGA TGCTGAAGAG GAAGAATACC GCCAGCAGCT GTCCGAACAC
    TCGAAGCGTG GCTATTACAG CCAGCCTTCC CGGTACCGGG ACACGGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270935.1
    CDS131..3544
    Translation

    Target ORF information:

    RefSeq Version XM_022415227.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAAC TGATATGGGA GCAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATCGTCATTT CTGACGTGCT ACCGGGTGGG CCTGCTGATG GGCTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTTAACGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCTGTCCAG
    CAGCTGAGAA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC CCCAGGGCAG CCTACCCGTC GATGAGGATG ACCGTGCTTT TGAGGTGATG
    GATGAGTTCG ATGGCAGAAG TGCCCGCAGC GGGTACAGCG AGAGGAGCCG GCGCAGCAGC
    CACGGTGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAGA GGGGGCGTCC CCATGAGCGG
    GCGTGGAGCC AGGAGCGCGA GCGCAGCCGT GGCCGGAGCC TGGAGCGGGG CCTGGACCAT
    GACGATGACT ACCGGCGGCC TCGAGAGCGC AGCCGGGGCC GGAGCCTGGA GCGGGGCCTG
    GACCATGACG ATGACTACGG GCGGCCCGGA GAGCGCAGCC ACGGCATGAG CACTGACAGG
    GGCTACGACC GTGGCTACGA CCGTGGCTAC GACCGTGGCT ACGACCGCAC CTATAGCCCA
    GAGGCCGAGT ATGGCCGCAG GGCCCAGCCT GATGCCCGGC ACGCAGGGTC CCGGAGCCGC
    AGCCGCGAGC ACTTGCGCTC CCGCAGCCCC AGCCCTGAGC TGAGGGGGCG GCCGGACCAT
    GCAGGCCAGC CTGACTCGGA CAGGCCCATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAGTACG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTGGCAACTA AAGATGGCAA CCTGCACGAA GGAGACATAA TTCTCAAGAT AAATGGAACT
    GTAACTGAGA ACATGTCTTT AACTGATGCC CGAAAACTGA TAGAAAAGTC AAGAGGAAAA
    CTCCAACTAG TGGTGTTGAG AGACAGCAAG CAAACGCTCA TCAACATTCC ATCATTAAAT
    GACAGCGACT CTGAAATAGA AGATATCTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GGCAGCAGTA TTCCGACTAT GATTATCATT CCTCAAATGA AAAACTGAAG
    GAAAGGCCAA ATTCAAGAGA GGACATGCAG AACAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCATGGG GGATATTGCA TCTGTGGTTG GCACAGAGAA CAGCAAGGAA
    CCCAGATACC AAGAGGAACC ACCAGCTCCT CAACCTAAAG CAGCCCCAAG AACTTTTCTT
    CGCCCGAGTC CTGAAGATGA AGCAATATAT GGTCCCAATA CCAAAATGGT GAGGTTTAAG
    AAGGGAGACA GCGTGGGACT CCGATTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCT
    GGTATTCAAG AGGGTACCTC GGCAGAACAA GAAGGCCTTC AAGAGGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGGCTA GTTCGGGAAG ATGCCGTTCT CTACCTGCTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGACTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCAGC AGGGGGGAGG TCTTCCGAGT TGTAGATACA
    CTGTATGATG GCAAGCTGGG CCACTGGCTG GCTGTGAGGA TTGGAAACGA ATTGGAGAAG
    GGCTTAATCC CCAACAAAAG CAGAGCTGAA CAAATGGCCA GTGTTCAGAA TGCCCAGAGA
    GACAATGCTG GGGACAGAGC AGATTTCTGG AGAATGCGTG GCCAGAGATC TGGCATGAAG
    AAGAACTTAA GGAAAAGTCG GGAAGATCTA ACAGCTGCTG TGTCAGTTAG CACCAAGTTC
    CCAGCTTACG AAAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTT
    GGCCCTATAG CAGATATAGC CTTGGAAAAG TTGGCAAATG AGTTACCGGA CCTGTTCCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCCGAGAAAT CTAGTGGGGT GGTCCGGTTA
    AATACTGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TATTGGATGT GACTCCTAAA
    GCTGTGGACC TGTTGAATTA TACTCAGTGG TTCCCGATTG TGATTTTTTT CAACCCAGAC
    TCTAGACAAG GTGTCAAAAC CATGAGACAG AGGTTGAATC CAACATCCAA CAAAAGTTCT
    CGGAAGTTGT ATGATCAAGC CAACAAGCTT AAGAAAACGT GTGCACATCT TTTTACAGCT
    ACTATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTGAAGGA CACAATTCAG
    CATCAGCAAG GAGAAGCAGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCTGAAGACC GCATGTCCTA CTTAACTGCC ATGGGCGCAG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCACTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGGCCAAAAC TCAGAACAGA GAAGAATCCT TTGACATCTC CAGATCCCAT
    GACTATAAGT CAAACCCCTC AGCCGTTGCT GGTAATGAAG TTTCTGGGGC ATCTACCAGA
    AGCTGTCCTC CTCCTATTGC AGCAAAACCT TCCTTTGGAA GGTCTATACT GAAGCCCTCC
    ACTCCTGTCC CTTCCCCAGA GAGTGAGGAG GTGGGAGAGG GCAGTGAAGA GCAAGAAGGC
    GCTCCCAAGT CTGTCCTGGG CAAAGTTAAA ATATTTGAGA AGATGGATCA CAAGGCAAGA
    TTACAGAGAA TGCAGGAGCT TCAAGAAGCA CAGAATGCAA GGATTGAAAT TGCTCAGAAG
    CATCCTGACA TCTATGCAGT TCCAATCAAA ACACACAAGC CAGACCCTGG CCTGTCCCAG
    CACACAAGTT CCAGGCCACC CGAGCCCCAG AAAGGCCCTT CCAGGCTCTA CCAGGATCCC
    AGAGGAAGTT ATGGCAGTGA TGCTGAAGAG GAAGAATACC GCCAGCAGCT GTCCGAACAC
    TCGAAGCGTG GCTATTACAG CCAGCCTTCC CGGTACCGGG ACACGGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.