PFKM cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKM
Entrez Gene ID	403849
Full Name	phosphofructokinase, muscle
Synonyms	ATP-PFK,M-PFK,PFK-M
General protein information	Preferred Names phosphofructokinase, muscle Names ATP-dependent 6-phosphofructokinase, muscle type 6-phosphofructokinase type A 6-phosphofructokinase, muscle type PFK-A muscle type phosphofructokinase phosphofructo-1-kinase isozyme A phosphofructokinase 1 phosphofructokinase-M phosphohexokinase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003199.1	NP_001003199.1	ATP-dependent 6-phosphofructokinase, muscle type

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

Missense mutation in PFKM associated with muscle-type phosphofructokinase deficiency in the Wachtelhund dog.
Inal Gultekin G, Raj K, Lehman S, Hillstr?m A, Giger U
Molecular and cellular probes26(6)243-7(2012 Dec)

Molecular basis of canine muscle type phosphofructokinase deficiency.
Smith BF, Stedman H, Rajpurohit Y, Henthorn PS, Wolfe JH, Patterson DF, Giger U
The Journal of biological chemistry271(33)20070-4(1996 Aug)

A cDNA encoding canine muscle-type phosphofructokinase.
Smith BF, Henthorn PS, Rajpurohit Y, Stedman H, Wolfe JH, Patterson DF, Giger U
Gene168(2)275-6(1996 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A point mutation in the PFKM coding sequence resulting in muscle-type phosphofructokinase deficiency in the Wachtelhund dog.
Title: Missense mutation in PFKM associated with muscle-type phosphofructokinase deficiency in the Wachtelhund dog.

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00405	NM_001003199.1 Latest version!	Canis lupus familiaris phosphofructokinase, muscle (PFKM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKM ( NM_001003199.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003199.1 Canis lupus familiaris phosphofructokinase, muscle (PFKM), mRNA.

Price & Availability↑

CloneID	OCe00405
Clone ID Related Accession (Same CDS sequence)	NM_001003199.1
Accession Version	NM_001003199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Canis lupus familiaris(dog)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U25183.1. ##Evidence-Data-START## Transcript exon combination :: U25183.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTG 
GGTATCTTTA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAGGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTCA TTGGAAGTGC CCGGTGTAAG GATTTTCGGG AACGAGAGGG GCGACTTCGA 
GCTGCCCACA ACCTAGTGAA ACGTGGGATC ACCAATCTGT GTGTCATCGG GGGTGATGGC 
AGCCTCACTG GGGCGGACAC CTTCCGTTCT GAGTGGAGTG ACTTGCTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC GGAGGAGGCT ACGAAGTCCA GCTACCTGAA TATCGTGGGC 
TTGGTGGGCT CAATTGACAA TGACTTTTGC GGCACTGACA TGACCATCGG CACCGACTCT 
GCCCTGCACC GGATCATAGA GATCGTGGAT GCCATCACCA CTACTGCTCA GAGCCACCAG 
AGGACGTTTG TGTTGGAAGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCC 
CTCTCCTGTG GGGCTGACTG GGTTTTTATT CCTGAATGTC CGCCAGATGA TGACTGGGAG 
GAACATCTTT GTCGCCGGCT TAGTGAGACA AGGACCCGGG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAAGAAT GGGAAACCAA TCACCTCAGA AGAAATCAAG 
GAGCTGGTGG TAAAGCGTCT GGGATATGAC ACTCGGGTCA CTGTCTTGGG GCATGTGCAG 
CGGGGTGGGA CCCCATCAGC CTTCGACCGA ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCGC TCTTGGAGGG GACCCCGGAC ACCCCGGCCT GCGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAG TGTGTACAGG TGACCAAGGA TGTGACCAAG 
GCCATGAATG ACAGGAAATT CGATGAAGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTGGCTCAC ATCAGACCTC CCGTCTCGAA GACATCGGCT 
ACCATGCACA CGGTGGCCGT GATGAACGTG GGTGCCCCAG CCGCAGGCAT GAATGCTGCC 
GTCCGCTCCA CCGTGAGAAT TGGACTCATC CAGGGCAACC GGGTGCTGGT TGTGCATGAT 
GGCTTCGAGG GCCTGGCCAA GGGTCAGATC GAGGAGGCTG GCTGGAGCTA TGTGGGGGGC 
TGGACTGGCC AAGGCGGTTC CAAACTTGGG ACTAAGAGGA CTCTGCCCAA GAAGAGCTTT 
GAGCAGATCA GTGCCAACAT CACTAAGTTT AACATTCAGG GCCTGATCAT CATCGGGGGC 
TTTGAGGCTT ACACTGGGGG CCTGGAGCTG ATGGAGGGCA GGAAGCAGTT TGATGAGCTG 
TGCATCCCGT TTGTGGTCAT CCCTGCTACG GTGTCCAACA ACGTCCCTGG CTCGGACTTC 
AGCGTGGGCG CTGACACAGC CCTCAACACT ATCTGCACGA CCTGTGACCG CATCAAGCAG 
TCAGCAGCAG GCACCAAGCG GCGGGTGTTC ATCATTGAAA CTATGGGTGG CTACTGCGGC 
TACCTGGCCA CCATGGCAGG CCTGGCCGCT GGGGCGGATG CTGCCTACAT TTTTGAGGAG 
CCCTTCACCA TTCGGGACCT GCAGGCAAAT GTTGAACATC TGGTGCAAAA GATGAAAACA 
ACTGTGAAGA GGGGCCTGGT GTTAAGGAAT GAGAAGTGCA ATGAGAACTA TACCACGGAC 
TTCATTTTCA ACCTGTACTC TGAGGAGGGG AAGGGCATCT TCGACAGCAG GAAGAATGTG 
CTCGGCCACA TGCAGCAGGG TGGGAGCCCA ACTCCATTTG ATAGGAATTT TGCCACTAAG 
ATGGGCGCCA AGGCTATGAA CTGGATGTCT GGGAAAATCA AAGAGAGTTA CCGTAATGGG 
AGGATCTTTG CCAATACGCC AGACTCGGGC TGTGTTCTGG GGATGCGTAA GAGGGCTCTG 
GTCTTTCAAC CAGTGACTGA GCTGAAGGAC CAGACAGATT TTGATCACCG CATCCCCAAG 
GAACAGTGGT GGCTGAAGCT GAGGCCCATC CTCAAAATCC TAGCCAAGTA CGAGATTGAC 
TTGGACACCA CAGAGCACGC CCACCTGGAG CACATCAGTC GGAAGCGATC TGGAGAAACT 
TCTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003199.1
CDS	23..2371
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEEIKELVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMNDRKFDEAMKLRGRSFMNNWEVYKLLAHIRPPVSKTSATMHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLIIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFDHRIPKEQWWLKLRPILKILAKYEIDLDTTEHAHLEHISRKRSGETSI

Target ORF information:

RefSeq Version	NM_001003199.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphofructokinase, muscle (PFKM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003199.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTG 
GGTATCTTTA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAGGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTCA TTGGAAGTGC CCGGTGTAAG GATTTTCGGG AACGAGAGGG GCGACTTCGA 
GCTGCCCACA ACCTAGTGAA ACGTGGGATC ACCAATCTGT GTGTCATCGG GGGTGATGGC 
AGCCTCACTG GGGCGGACAC CTTCCGTTCT GAGTGGAGTG ACTTGCTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC GGAGGAGGCT ACGAAGTCCA GCTACCTGAA TATCGTGGGC 
TTGGTGGGCT CAATTGACAA TGACTTTTGC GGCACTGACA TGACCATCGG CACCGACTCT 
GCCCTGCACC GGATCATAGA GATCGTGGAT GCCATCACCA CTACTGCTCA GAGCCACCAG 
AGGACGTTTG TGTTGGAAGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCC 
CTCTCCTGTG GGGCTGACTG GGTTTTTATT CCTGAATGTC CGCCAGATGA TGACTGGGAG 
GAACATCTTT GTCGCCGGCT TAGTGAGACA AGGACCCGGG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAAGAAT GGGAAACCAA TCACCTCAGA AGAAATCAAG 
GAGCTGGTGG TAAAGCGTCT GGGATATGAC ACTCGGGTCA CTGTCTTGGG GCATGTGCAG 
CGGGGTGGGA CCCCATCAGC CTTCGACCGA ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCGC TCTTGGAGGG GACCCCGGAC ACCCCGGCCT GCGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAG TGTGTACAGG TGACCAAGGA TGTGACCAAG 
GCCATGAATG ACAGGAAATT CGATGAAGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTGGCTCAC ATCAGACCTC CCGTCTCGAA GACATCGGCT 
ACCATGCACA CGGTGGCCGT GATGAACGTG GGTGCCCCAG CCGCAGGCAT GAATGCTGCC 
GTCCGCTCCA CCGTGAGAAT TGGACTCATC CAGGGCAACC GGGTGCTGGT TGTGCATGAT 
GGCTTCGAGG GCCTGGCCAA GGGTCAGATC GAGGAGGCTG GCTGGAGCTA TGTGGGGGGC 
TGGACTGGCC AAGGCGGTTC CAAACTTGGG ACTAAGAGGA CTCTGCCCAA GAAGAGCTTT 
GAGCAGATCA GTGCCAACAT CACTAAGTTT AACATTCAGG GCCTGATCAT CATCGGGGGC 
TTTGAGGCTT ACACTGGGGG CCTGGAGCTG ATGGAGGGCA GGAAGCAGTT TGATGAGCTG 
TGCATCCCGT TTGTGGTCAT CCCTGCTACG GTGTCCAACA ACGTCCCTGG CTCGGACTTC 
AGCGTGGGCG CTGACACAGC CCTCAACACT ATCTGCACGA CCTGTGACCG CATCAAGCAG 
TCAGCAGCAG GCACCAAGCG GCGGGTGTTC ATCATTGAAA CTATGGGTGG CTACTGCGGC 
TACCTGGCCA CCATGGCAGG CCTGGCCGCT GGGGCGGATG CTGCCTACAT TTTTGAGGAG 
CCCTTCACCA TTCGGGACCT GCAGGCAAAT GTTGAACATC TGGTGCAAAA GATGAAAACA 
ACTGTGAAGA GGGGCCTGGT GTTAAGGAAT GAGAAGTGCA ATGAGAACTA TACCACGGAC 
TTCATTTTCA ACCTGTACTC TGAGGAGGGG AAGGGCATCT TCGACAGCAG GAAGAATGTG 
CTCGGCCACA TGCAGCAGGG TGGGAGCCCA ACTCCATTTG ATAGGAATTT TGCCACTAAG 
ATGGGCGCCA AGGCTATGAA CTGGATGTCT GGGAAAATCA AAGAGAGTTA CCGTAATGGG 
AGGATCTTTG CCAATACGCC AGACTCGGGC TGTGTTCTGG GGATGCGTAA GAGGGCTCTG 
GTCTTTCAAC CAGTGACTGA GCTGAAGGAC CAGACAGATT TTGATCACCG CATCCCCAAG 
GAACAGTGGT GGCTGAAGCT GAGGCCCATC CTCAAAATCC TAGCCAAGTA CGAGATTGAC 
TTGGACACCA CAGAGCACGC CCACCTGGAG CACATCAGTC GGAAGCGATC TGGAGAAACT 
TCTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Missense mutation in PFKM associated with muscle-type phosphofructokinase deficiency in the Wachtelhund dog.
Molecular and cellular probes26(6)243-7(2012 Dec)
Inal Gultekin G,Raj K,Lehman S,Hillstr?m A,Giger U

Molecular basis of canine muscle type phosphofructokinase deficiency.
The Journal of biological chemistry271(33)20070-4(1996 Aug)
Smith BF,Stedman H,Rajpurohit Y,Henthorn PS,Wolfe JH,Patterson DF,Giger U

A cDNA encoding canine muscle-type phosphofructokinase.
Gene168(2)275-6(1996 Feb)
Smith BF,Henthorn PS,Rajpurohit Y,Stedman H,Wolfe JH,Patterson DF,Giger U

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PFKM cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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