COL4A4 cDNA ORF clone, Canis lupus familiaris(dog)

The following COL4A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe34069 NM_001031818.1
Latest version!
Canis lupus familiaris collagen type IV alpha 4 chain (COL4A4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe25516 XM_005635602.3
Latest version!
Canis lupus familiaris collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe25516 XM_005635603.2
Latest version!
Canis lupus familiaris collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe25518 XM_005635606.2
Latest version!
Canis lupus familiaris collagen, type IV, alpha 4 (COL4A4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe297002 XM_022409541.1
Latest version!
Canis lupus familiaris collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OCe25516 XM_005635602.2 Canis lupus familiaris collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe34069
    Clone ID Related Accession (Same CDS sequence) NM_001031818.1
    Accession Version NM_001031818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-21
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-4(IV) chain
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY263363.1. On Aug 19, 2005 this sequence version replaced XM_534589.1. ##Evidence-Data-START## Transcript exon combination :: AY263363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAG GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCAGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGTGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCCGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GACTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCTCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTTGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCCAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCANGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCNT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGNCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCNGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACNCCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCCGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGANGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001026988.1
    CDS94..5160
    Translation

    Target ORF information:

    RefSeq Version NM_001031818.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 4 chain (COL4A4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001031818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAG GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCAGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGTGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCCGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GACTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCTCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTTGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCCAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCANGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCNT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGNCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCNGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACNCCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCCGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGANGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25516
    Clone ID Related Accession (Same CDS sequence) XM_005635603.2 , XM_005635602.3 , XM_005635602.2
    Accession Version XM_005635603.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-4(IV) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAA GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCGGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGGGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCTGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GGCTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCCCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTCGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCAAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCAGGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCCT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGTCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCAGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACACCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCTGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGACGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635660.1
    CDS469..5535
    Translation

    Target ORF information:

    RefSeq Version XM_005635603.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635603.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAA GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCGGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGGGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCTGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GGCTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCCCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTCGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCAAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCAGGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCCT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGTCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCAGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACACCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCTGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGACGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25516
    Clone ID Related Accession (Same CDS sequence) XM_005635603.2 , XM_005635602.3 , XM_005635602.2
    Accession Version XM_005635602.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635602.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAA GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCGGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGGGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCTGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GGCTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCCCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTCGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCAAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCAGGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCCT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGTCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCAGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACACCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCTGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGACGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635659.1
    CDS342..5408
    Translation

    Target ORF information:

    RefSeq Version XM_005635602.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635602.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAA GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCGGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGGGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCTGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GGCTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCCCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTCGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCAAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCAGGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCCT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGTCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCAGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACACCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCTGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGACGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25516
    Clone ID Related Accession (Same CDS sequence) XM_005635603.2 , XM_005635602.3 , XM_005635602.2
    Accession Version XM_005635602.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-4(IV) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAA GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCGGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGGGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCTGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GGCTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCCCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTCGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCAAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCAGGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCCT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGTCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCAGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACACCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCTGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGACGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635659.1
    CDS357..5423
    Translation

    Target ORF information:

    RefSeq Version XM_005635602.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635602.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACATGG CATCAGTAAA GTGTTCATTC AGGTGGATCA AGCCCTTGGC CAGAGATCCC 
    TGGTCACTTA TATTTATCCT CTTTTCTGTA CAACATGCAT ATGGGAGTGG AAAGAAGTTT
    GTCGGCCCCT GTGGAGGAAG AGATTGCTCG GTGTGCCATT GCTTTCCTGA AAAAGGGTCT
    CGGGGTCAAC CAGGACCGCC GGGGCCACAG GGTCCCATTG GACCCCTGGG ACTGCCAGGA
    CCCACAGGAA TTCCAGGAGA GAAAGGGATG AGAGGTGACA GTGGCCCTCC TGGAGCAGCA
    GGTGACAAAG GTGACAAGGG TCCAACTGGT GTTCCTGGAT TTCCAGGGTT GGATGGCATA
    CCTGGCCACC CGGGGCCTCC TGGATCCAGA GGCAAGCCTG GCATGCATGG CTACAATGGT
    TCACGAGGTG ATCCAGGGTT TCCAGGAGAA AGAGGAGTTC CTGGCCCAGG AGGCCCCCCA
    GGCCTTCCTG GTGAAAGGGG AGAAAAAGGA AATTCAGTGT TCATTTTAGG TGCCATTAAA
    GGTATTCAGG GTGACAGGGG GGACCCAGGA CCTCCTGGCT TGCCGGGACC GAAGGGGGCA
    CGAGGACCGG CAGGCCCAAG GGGACAGCCA GGAGAGCCTG GGTTAACAGG TGCTCCGGGC
    CATCCTGGGA GACCAGGCTT GAAGGGTAAT CCTGGTGTGG GAGTAAAGGG GCAAATGGGA
    GACCCGGGTG AGGTTGGCCA GCAGGGTTCT CCTGGACCCA CCTTATTGGT ACAGCCACCT
    GATTCCTGTC TGTATAAAGG AGAAAAGGGC ATAAAAGGAA TGCCTGGTAT GATTGGACCT
    CCAGGACTAC CGGGACCCAA GGGAGAACCT GGAATTGGAC CAAAAGGAGA GAAGGGTATT
    CCTGGGTTCT CAGGACCTCG GGGTGATCCG GGTTCCTATG GCTCTCCAGG TTTTCCAGGA
    TTAAAGGGGA AACCAGGACT GTTTGGAGAT CCTGGATCAT TTGGATTTGT TGGCCCAAAG
    GGGGATCCTG GAGACCGTGG GTACCCAGGA CCACCGGGTG TTCTGGTAAC TCCATCTGTT
    CCACTCAAAG GCCCTCCAGG GGATCCGGGG CGCCCTGGCC ACTATGGAGA AATGGGGTCC
    GTTGGACCAC CTGGTCCCCC TGGTCCCCCA GGTCCACCAG GGGAAGACTG TGCAGGCATG
    ATGGGACCCC CTGGACCAAG AGGATTTCCT GGTCATCCAG GATTTCCAGG GGCAGCTGGT
    ATCCCAGGGA GAGCGGATTT AAGTCCAGGA AAACCAGGGA AACCGGGACC TCCTGGGTTG
    CCCGGAGCAC CAGGGCTGCA AGGACCCCCA GGATCAGATG TTATCTATTG TAGTGTTGGG
    CATCCTGGAC CACAAGGGAT AAAAGGCAAA ATGGGTCCTC CAGGAAGAAG AGGCTCGAAA
    GGAGAAAAAG GAAATGCGGG GCTCTGTGCC TGTGAGCCTG GTCCCATGGG CCCACCAGGC
    CCTCCAGGAC TTCCTGGAAG GCAGGGTAGT AAGGGAGACT TGGGGCTCCC TGGGTGGATT
    GGAGAGAAAG GTCACCCAGG CCCTCCTGGT GCTGAAGGAT CTCCAGGATC ACCAGGAAAA
    CATGGTGCCT CAGGACCACC TGGCAGCAAA GGAGAAAAGG GCGACAGGGT TGTATCAAGA
    GTGAAAGGGA AAAAAGGAGA AAGAGGTCCT GATGGGCTCC CAGGATTTCC AGGACAACAG
    GGACAAAATG GTCGGGATGG ACTTCCTGGA AAAAAGGGGG ATCCAGGCCC CCCGGGGGGT
    CATGAGGATG CAGCCCCAGG TGCTAAAGGG TCTCCTGGAC CACCGGGGCC CCCAGGCAGA
    ACAGGACCCA GGGGACAACC AGGTCTGGGA TTTCCTGGTC CACCAGGAGA GAGAGGACAA
    CCAGGAGCTC CAGGCCGCCC TGGCGAGAGG GGCTTCGGGG GCTTGAAGGG CCAGAAAGGT
    GATACAATTT CTTGTAACGT CACCTACCCT GGGAGGCCGG GGCCCCCAGG TTTTGATGGA
    CCTCCAGGAC CAAAGGGATT TCCAGGTCCT CAAGGCGCTC CAGGGTTGAG GTGCTTGGAT
    GGGCCAAAAG GTCAGCGTGG CAAACCAGGA ATGTCAGAGA TACCCGGTCC ACCTGGTTTT
    CGTGGTGACA TGGGAGATCC GGGTTTTGAA GGTGAAAAGG GGTCCTCCCT TCTTGGGCCG
    CCAGGCTTTC CTGGTTCTCG TGGAGCAAAT GGTCAGAAAG GAATGATGGG AGACATTGCC
    TATGGCCTCC CAGGCCCCCC AGGAAAGAGA GGTCCTTCCG GAGTGCCAGG GTCCAAAGGA
    CACAGAGGTG ATTCAGGACG TCCAGGGTTT GCAGGGCCAG CCGGCAAGCC TGGATCCCCA
    GGTCTCAAAG GTCCCAGAGG CAGAGAGGGA AGTGCTGGGT TTCAAGGTAT CCCGGGTCCA
    CCTGGCCATT CCTGCAAAAG AGGCGCTCCA GGGATACCAG GGCAACCGGG GCTCCCTGGG
    GCCCCGGGGA GTCCAGGTGC CCCAGGTTGG AAAGGACAGC GAGGGGATGT GGGGCCTCCG
    GGTCCAGCTG GAATGAAAGG CCTCCCTGGA GTCCCTGGAC GGCCAGGGGC AGAGGGACCC
    CTAGGATTCC CAGGAGTCCC AGGCCCCTTA GGGGATGATG GGCTGCCTGG TCTTCCAGGC
    CCGAAGGGAC CCCAGGGGCT GCCTGGCTTC CCCGGTTTTC CAGGGGAGAG AGGAAAGCCT
    GGCCCAGAGG GACGCCCTGG CAGCAAGGGG GAACCAGGAG AGGATGGTCG GCCTGGCTTC
    TTCGGAGACC AAGGGGTGAA AGGTGCCAAA GGAGCGAGAG GACCCCCAGG AGATGAAGGA
    GAGATGGCGG TCTTTTCCCA AAAAGGGAAA ACCGGGGAAC CTGGACCTCC AGGAGATGAT
    GGATTCCCAG GAGAAGAAGG TGATAAAGGC AATCCAGGGA TGCCGGGGAG GAGAGGAGAG
    CCTGGAAGAT TCGGAGCACC TGGATTTCAC AGAGGGGAGC CCGGGAGAAC CGGGCAGCCA
    GGGCTTCCTG GCCCCCCAGG CCCCCCAGGC TCACCTGGGC TGAGAGGGAT TATTGGTTTT
    CCGGGATTTC CAGGTGACCA GGGTGAGCCA GGTTCTCCAG GGTCTCCTGG ACTTTCAGGA
    ATTGATGGAA TGAGAGGACC TAAAGGAAGC AAAGGTGACC CTGCAAGTCA GTTCGGCCCA
    CCTGGTCCAA AGGGTGAACC AGGTAGCCCT GGATGTCCAG GACAAGTTGG AGCACCTGGA
    GAGCAGGGCT TGCCTGGTGT TCAAGGGCTC AGAGGACCAC CTGGAAGGCC AGGATTACCT
    GGCTCCTCCG GACCACCAGG GTGTCCAGGT AATCAAGGGG TGCCTGGGCT GACAGGGCCT
    CCAGGAGAAA CAGGGGATCC TGGGCCAAGA GGCATGATGG GAGATCCAGG GCCACCAGGT
    CTTCCAGGAA TAAAAGGTCC CTCCGGGTCG CCGGGTCTGA ATGGCTTGCA TGGTTTAAAG
    GGTCAGAAAG GAGCCAAAGG TGCTTCAGGT TTGCACGAAG TGGGCCCACC CGGTCCAGTG
    GGCGTACCTG GGCTGAAAGG GGAGCCGGGA GACCCTGGGA GCCCAGGAAT TTCTCCCCCA
    GGCCTTTCTG GAGAAAGAGG CCCCCCCGGC CCCCCAGGGA GACCTGGAGC CCCTGGTCCT
    GCAGGTGCCA CAGGAAGAGC TGCTGAAGGT CACGTTCCTG ACCCAGGTCC ACCCGGAGAT
    GTGGGTCCTC CTGGCCCCGA TGGTCCGAGA GGAGCGCCCG GGCCCCCAGG CCCCCCCGGG
    AGCGTTGACC TTCTGAGAGG GGAACCAGGA GACTGTGGGC CGCCGGGGCC TCCAGGTCCC
    CCGGGCCCAC CCGGCCCTCC AGGACACCAA GGCTTCCCAG GATGCGATGG AAAAGACGGC
    CAGAAAGGAC CAATAGGATT CCCGGGGCTG CAGGGGCCTC AGGGACTTCC TGGGCTCCCT
    GGGGAGAAGG GTCTGCTGGG CATTCCAGGC CGGCGAGGGC ACCCCGGTCC TCCAGGTTCC
    AGAGGTGAAC CAGGGCCTCC TGCAGATGTG GATTCGTGCC CCCGCATCCC CGGGCTTCCT
    GGGGTACCAG GCCCAAGAGG ACCAGAAGGA GCCATGGGGG TCCCTGGAAG GAGAGGGCCC
    CCAGGACCAG GGTGCAAAGG AGAGCCGGGA CTGGAGGGCA GGAGGGGCGA GGCCGGCCTC
    CCAGGACCTC CGGGGCCTCC CGGAAGCACA GGGGACGCAG GAGAAGCCGG TTGCCCCGGA
    GCACCAGGGC CCCCCGGGCC CATTGGGGAC CCTGGGCCCA GAGGGTTTGG GCCTGGATAC
    CTCAGTGGCT TCCTCCTGGT TCTCCACAGT CAGACGGATG GGGAGCCCAC CTGCCCCGCG
    GGCATGCCCA GGCTCTGGAC GGGCTACAGT CTGTTATACC TGGAAGGACA GGAGAAGGCC
    CACAATCAGG ACCTTGGTCT GGCAGGGTCT TGCCTTCCCA TGTTCAGCAC CCTGCCCTTT
    GCCTACTGCA ACATCCACCA AGTGTGCCAC TACGCCCGGA GGAACGACCG GTCCTACTGG
    CTGGCCAGTG CTGCGCCCCT GCCCATGATG CCGCTCTCAG AGGAGGAGAT CCGCCCCTAC
    ATCAGCCGCT GTGCCGTGTG CGAGGCCCCA GCCCCCGTGG TGGCGCTGCA CAGCCAGGAC
    CGGTCCATCC CGCCGTGTCC ACGGAGCTGG AGGAGCCTCT GGATCGGGTA CTCGTTCCTG
    ATGCACACAG GGGCTGGAGA CCAAGGAGGA GGGCAGGCCC TCATGTCCCC CGGCAGCTGT
    CTGGAAGATT TCCGAGCCGC ACCGTTCCTC GAATGCCAAG GCCGACAGGG AACTTGCCAC
    TTTTTTGCAA ATGAGTATAG CTTCTGGCTG ACGACGGTGA GGCCTGACTT GCAGTTTTCC
    TCGGCACCAT CACCAGACAC CTTGAAAGAA AGCCAGGCCC AGCGCCAGAG AATCAGCAGG
    TGCCAGGTCT GCATGAAGTA TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25518
    Clone ID Related Accession (Same CDS sequence) XM_005635606.2
    Accession Version XM_005635606.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-4(IV) chain isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGCAAC AGAGCAGGAA AGACCCCTGT GATATAAATA CGAGTGGAAA GAAGTTTGTC 
    GGCCCCTGTG GAGGAAGAGA TTGCTCGGTG TGCCATTGCT TTCCTGAAAA AGGGTCTCGG
    GGTCAACCAG GACCGCCGGG GCCACAGGGT CCCATTGGAC CCCTGGGACT GCCAGGACCC
    ACAGGAATTC CAGGAGAGAA AGGGATGAGA GGTGACAGTG GCCCTCCTGG AGCAGCAGGT
    GACAAAGGTG ACAAGGGTCC AACTGGTGTT CCTGGATTTC CAGGGTTGGA TGGCATACCT
    GGCCACCCGG GGCCTCCTGG ATCCAGAGGC AAGCCTGGCA TGCATGGCTA CAATGGTTCA
    CGAGGTGATC CAGGGTTTCC AGGAGAAAGA GGAGTTCCTG GCCCAGGAGG CCCCCCAGGC
    CTTCCTGGTG AAAGGGGAGA AAAAGGAAAT TCAGTGTTCA TTTTAGGTGC CATTAAAGGT
    ATTCAGGGTG ACAGGGGGGA CCCAGGACCT CCTGGCTTGC CGGGACCGAA GGGGGCACGA
    GGACCGGCAG GCCCAAGGGG ACAGCCAGGA GAGCCTGGGT TAACAGGTGC TCCGGGCCAT
    CCTGGGAGAC CAGGCTTGAA GGGTAATCCT GGTGTGGGAG TAAAGGGGCA AATGGGAGAC
    CCGGGTGAGG TTGGCCAGCA GGGTTCTCCT GGACCCACCT TATTGGTACA GCCACCTGAT
    TCCTGTCTGT ATAAAGGAGA AAAGGGCATA AAAGGAATGC CTGGTATGAT TGGACCTCCA
    GGACTACCGG GACCCAAGGG AGAACCTGGA ATTGGACCAA AAGGAGAGAA GGGTATTCCT
    GGGTTCTCAG GACCTCGGGG TGATCCGGGT TCCTATGGCT CTCCAGGTTT TCCAGGATTA
    AAGGGGAAAC CAGGACTGTT TGGAGATCCT GGATCATTTG GATTTGTTGG CCCAAAGGGG
    GATCCTGGAG ACCGTGGGTA CCCAGGACCA CCGGGTGTTC TGGTAACTCC ATCTGTTCCA
    CTCAAAGGCC CTCCAGGGGA TCCGGGGCGC CCTGGCCACT ATGGAGAAAT GGGGTCCGTT
    GGACCACCTG GTCCCCCTGG TCCCCCAGGT CCACCAGGGG AAGACTGTGC AGGCATGATG
    GGACCCCCTG GACCAAGAGG ATTTCCTGGT CATCCAGGAT TTCCAGGGGC AGCTGGTATC
    CCAGGGAGAG CGGATTTAAG TCCAGGAAAA CCAGGGAAAC CGGGACCTCC TGGGTTGCCC
    GGAGCACCAG GGCTGCAAGG ACCCCCAGGA TCAGATGTTA TCTATTGTAG TGTTGGGCAT
    CCTGGACCAC AAGGGATAAA AGGCAAAATG GGTCCTCCAG GAAGAAGAGG CTCGAAAGGA
    GAAAAAGGAA ATGCGGGGCT CTGTGCCTGT GAGCCTGGTC CCATGGGCCC ACCAGGCCCT
    CCAGGACTTC CTGGAAGGCA GGGTAGTAAG GGAGACTTGG GGCTCCCTGG GTGGATTGGA
    GAGAAAGGTC ACCCAGGCCC TCCTGGTGCT GAAGGATCTC CAGGATCACC AGGAAAACAT
    GGTGCCTCAG GACCACCTGG CAGCAAAGGA GAAAAGGGCG ACAGGGTTGT ATCAAGAGTG
    AAAGGGAAAA AAGGAGAAAG AGGTCCTGAT GGGCTCCCAG GATTTCCAGG ACAACAGGGA
    CAAAATGGTC GGGATGGACT TCCTGGAAAA AAGGGGGATC CAGGCCCCCC GGGGGGTCAT
    GAGGATGCAG CCCCAGGTGC TAAAGGGTCT CCTGGACCAC CGGGGCCCCC AGGCAGAACA
    GGACCCAGGG GACAACCAGG TCTGGGATTT CCTGGTCCAC CAGGAGAGAG AGGACAACCA
    GGAGCTCCAG GCCGCCCTGG CGAGAGGGGC TTCGGGGGCT TGAAGGGCCA GAAAGGTGAT
    ACAATTTCTT GTAACGTCAC CTACCCTGGG AGGCCGGGGC CCCCAGGTTT TGATGGACCT
    CCAGGACCAA AGGGATTTCC AGGTCCTCAA GGCGCTCCAG GGTTGAGGTG CTTGGATGGG
    CCAAAAGGTC AGCGTGGCAA ACCAGGAATG TCAGAGATAC CCGGTCCACC TGGTTTTCGT
    GGTGACATGG GAGATCCGGG TTTTGAAGGT GAAAAGGGGT CCTCCCTTCT TGGGCCGCCA
    GGCTTTCCTG GTTCTCGTGG AGCAAATGGT CAGAAAGGAA TGATGGGAGA CATTGCCTAT
    GGCCTCCCAG GCCCCCCAGG AAAGAGAGGT CCTTCCGGAG TGCCAGGGTC CAAAGGACAC
    AGAGGTGATT CAGGACGTCC AGGGTTTGCA GGGCCAGCCG GCAAGCCTGG ATCCCCAGGT
    CTCAAAGGTC CCAGAGGCAG AGAGGGAAGT GCTGGGTTTC AAGGTATCCC GGGTCCACCT
    GGCCATTCCT GCAAAAGAGG CGCTCCAGGG ATACCAGGGC AACCGGGGCT CCCTGGGGCC
    CCGGGGAGTC CAGGTGCCCC AGGTTGGAAA GGACAGCGAG GGGATGTGGG GCCTCCGGGT
    CCAGCTGGAA TGAAAGGCCT CCCTGGAGTC CCTGGACGGC CAGGGGCAGA GGGACCCCTA
    GGATTCCCAG GAGTCCCAGG CCCCTTAGGG GATGATGGGC TGCCTGGTCT TCCAGGCCCG
    AAGGGACCCC AGGGGCTGCC TGGCTTCCCC GGTTTTCCAG GGGAGAGAGG AAAGCCTGGC
    CCAGAGGGAC GCCCTGGCAG CAAGGGGGAA CCAGGAGAGG ATGGTCGGCC TGGCTTCTTC
    GGAGACCAAG GGGTGAAAGG TGCCAAAGGA GCGAGAGGAC CCCCAGGAGA TGAAGGAGAG
    ATGGCGGTCT TTTCCCAAAA AGGGAAAACC GGGGAACCTG GACCTCCAGG AGATGATGGA
    TTCCCAGGAG AAGAAGGTGA TAAAGGCAAT CCAGGGATGC CGGGGAGGAG AGGAGAGCCT
    GGAAGATTCG GAGCACCTGG ATTTCACAGA GGGGAGCCCG GGAGAACCGG GCAGCCAGGG
    CTTCCTGGCC CCCCAGGCCC CCCAGGCTCA CCTGGGCTGA GAGGGATTAT TGGTTTTCCG
    GGATTTCCAG GTGACCAGGG TGAGCCAGGT TCTCCAGGGT CTCCTGGACT TTCAGGAATT
    GATGGAATGA GAGGACCTAA AGGAAGCAAA GGTGACCCTG CAAGTCAGTT CGGCCCACCT
    GGTCCAAAGG GTGAACCAGG TAGCCCTGGA TGTCCAGGAC AAGTTGGAGC ACCTGGAGAG
    CAGGGCTTGC CTGGTGTTCA AGGGCTCAGA GGACCACCTG GAAGGCCAGG ATTACCTGGC
    TCCTCCGGAC CACCAGGGTG TCCAGGTAAT CAAGGGGTGC CTGGGCTGAC AGGGCCTCCA
    GGAGAAACAG GGGATCCTGG GCCAAGAGGC ATGATGGGAG ATCCAGGGCC ACCAGGTCTT
    CCAGGAATAA AAGGTCCCTC CGGGTCGCCG GGTCTGAATG GCTTGCATGG TTTAAAGGGT
    CAGAAAGGAG CCAAAGGTGC TTCAGGTTTG CACGAAGTGG GCCCACCCGG TCCAGTGGGC
    GTACCTGGGC TGAAAGGGGA GCCGGGAGAC CCTGGGAGCC CAGGAATTTC TCCCCCAGGC
    CTTTCTGGAG AAAGAGGCCC CCCCGGCCCC CCAGGGAGAC CTGGAGCCCC TGGTCCTGCA
    GGTGCCACAG GAAGAGCTGC TGAAGGTCAC GTTCCTGACC CAGGTCCACC CGGAGATGTG
    GGTCCTCCTG GCCCCGATGG TCCGAGAGGA GCGCCCGGGC CCCCAGGCCC CCCCGGGAGC
    GTTGACCTTC TGAGAGGGGA ACCAGGAGAC TGTGGGCCGC CGGGGCCTCC AGGTCCCCCG
    GGCCCACCCG GCCCTCCAGG ACACCAAGGC TTCCCAGGAT GCGATGGAAA AGACGGCCAG
    AAAGGACCAA TAGGATTCCC GGGGCTGCAG GGGCCTCAGG GACTTCCTGG GCTCCCTGGG
    GAGAAGGGTC TGCTGGGCAT TCCAGGCCGG CGAGGGCACC CCGGTCCTCC AGGTTCCAGA
    GGTGAACCAG GGCCTCCTGC AGATGTGGAT TCGTGCCCCC GCATCCCCGG GCTTCCTGGG
    GTACCAGGCC CAAGAGGACC AGAAGGAGCC ATGGGGGTCC CTGGAAGGAG AGGGCCCCCA
    GGACCAGGGT GCAAAGGAGA GCCGGGACTG GAGGGCAGGA GGGGCGAGGC CGGCCTCCCA
    GGACCTCCGG GGCCTCCCGG AAGCACAGGG GACGCAGGAG AAGCCGGTTG CCCCGGAGCA
    CCAGGGCCCC CCGGGCCCAT TGGGGACCCT GGGCCCAGAG GGTTTGGGCC TGGATACCTC
    AGTGGCTTCC TCCTGGTTCT CCACAGTCAG ACGGATGGGG AGCCCACCTG CCCCGCGGGC
    ATGCCCAGGC TCTGGACGGG CTACAGTCTG TTATACCTGG AAGGACAGGA GAAGGCCCAC
    AATCAGGACC TTGGTCTGGC AGGGTCTTGC CTTCCCATGT TCAGCACCCT GCCCTTTGCC
    TACTGCAACA TCCACCAAGT GTGCCACTAC GCCCGGAGGA ACGACCGGTC CTACTGGCTG
    GCCAGTGCTG CGCCCCTGCC CATGATGCCG CTCTCAGAGG AGGAGATCCG CCCCTACATC
    AGCCGCTGTG CCGTGTGCGA GGCCCCAGCC CCCGTGGTGG CGCTGCACAG CCAGGACCGG
    TCCATCCCGC CGTGTCCACG GAGCTGGAGG AGCCTCTGGA TCGGGTACTC GTTCCTGATG
    CACACAGGGG CTGGAGACCA AGGAGGAGGG CAGGCCCTCA TGTCCCCCGG CAGCTGTCTG
    GAAGATTTCC GAGCCGCACC GTTCCTCGAA TGCCAAGGCC GACAGGGAAC TTGCCACTTT
    TTTGCAAATG AGTATAGCTT CTGGCTGACG ACGGTGAGGC CTGACTTGCA GTTTTCCTCG
    GCACCATCAC CAGACACCTT GAAAGAAAGC CAGGCCCAGC GCCAGAGAAT CAGCAGGTGC
    CAGGTCTGCA TGAAGTATGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635663.1
    CDS220..5223
    Translation

    Target ORF information:

    RefSeq Version XM_005635606.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 4 (COL4A4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635606.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGCAAC AGAGCAGGAA AGACCCCTGT GATATAAATA CGAGTGGAAA GAAGTTTGTC 
    GGCCCCTGTG GAGGAAGAGA TTGCTCGGTG TGCCATTGCT TTCCTGAAAA AGGGTCTCGG
    GGTCAACCAG GACCGCCGGG GCCACAGGGT CCCATTGGAC CCCTGGGACT GCCAGGACCC
    ACAGGAATTC CAGGAGAGAA AGGGATGAGA GGTGACAGTG GCCCTCCTGG AGCAGCAGGT
    GACAAAGGTG ACAAGGGTCC AACTGGTGTT CCTGGATTTC CAGGGTTGGA TGGCATACCT
    GGCCACCCGG GGCCTCCTGG ATCCAGAGGC AAGCCTGGCA TGCATGGCTA CAATGGTTCA
    CGAGGTGATC CAGGGTTTCC AGGAGAAAGA GGAGTTCCTG GCCCAGGAGG CCCCCCAGGC
    CTTCCTGGTG AAAGGGGAGA AAAAGGAAAT TCAGTGTTCA TTTTAGGTGC CATTAAAGGT
    ATTCAGGGTG ACAGGGGGGA CCCAGGACCT CCTGGCTTGC CGGGACCGAA GGGGGCACGA
    GGACCGGCAG GCCCAAGGGG ACAGCCAGGA GAGCCTGGGT TAACAGGTGC TCCGGGCCAT
    CCTGGGAGAC CAGGCTTGAA GGGTAATCCT GGTGTGGGAG TAAAGGGGCA AATGGGAGAC
    CCGGGTGAGG TTGGCCAGCA GGGTTCTCCT GGACCCACCT TATTGGTACA GCCACCTGAT
    TCCTGTCTGT ATAAAGGAGA AAAGGGCATA AAAGGAATGC CTGGTATGAT TGGACCTCCA
    GGACTACCGG GACCCAAGGG AGAACCTGGA ATTGGACCAA AAGGAGAGAA GGGTATTCCT
    GGGTTCTCAG GACCTCGGGG TGATCCGGGT TCCTATGGCT CTCCAGGTTT TCCAGGATTA
    AAGGGGAAAC CAGGACTGTT TGGAGATCCT GGATCATTTG GATTTGTTGG CCCAAAGGGG
    GATCCTGGAG ACCGTGGGTA CCCAGGACCA CCGGGTGTTC TGGTAACTCC ATCTGTTCCA
    CTCAAAGGCC CTCCAGGGGA TCCGGGGCGC CCTGGCCACT ATGGAGAAAT GGGGTCCGTT
    GGACCACCTG GTCCCCCTGG TCCCCCAGGT CCACCAGGGG AAGACTGTGC AGGCATGATG
    GGACCCCCTG GACCAAGAGG ATTTCCTGGT CATCCAGGAT TTCCAGGGGC AGCTGGTATC
    CCAGGGAGAG CGGATTTAAG TCCAGGAAAA CCAGGGAAAC CGGGACCTCC TGGGTTGCCC
    GGAGCACCAG GGCTGCAAGG ACCCCCAGGA TCAGATGTTA TCTATTGTAG TGTTGGGCAT
    CCTGGACCAC AAGGGATAAA AGGCAAAATG GGTCCTCCAG GAAGAAGAGG CTCGAAAGGA
    GAAAAAGGAA ATGCGGGGCT CTGTGCCTGT GAGCCTGGTC CCATGGGCCC ACCAGGCCCT
    CCAGGACTTC CTGGAAGGCA GGGTAGTAAG GGAGACTTGG GGCTCCCTGG GTGGATTGGA
    GAGAAAGGTC ACCCAGGCCC TCCTGGTGCT GAAGGATCTC CAGGATCACC AGGAAAACAT
    GGTGCCTCAG GACCACCTGG CAGCAAAGGA GAAAAGGGCG ACAGGGTTGT ATCAAGAGTG
    AAAGGGAAAA AAGGAGAAAG AGGTCCTGAT GGGCTCCCAG GATTTCCAGG ACAACAGGGA
    CAAAATGGTC GGGATGGACT TCCTGGAAAA AAGGGGGATC CAGGCCCCCC GGGGGGTCAT
    GAGGATGCAG CCCCAGGTGC TAAAGGGTCT CCTGGACCAC CGGGGCCCCC AGGCAGAACA
    GGACCCAGGG GACAACCAGG TCTGGGATTT CCTGGTCCAC CAGGAGAGAG AGGACAACCA
    GGAGCTCCAG GCCGCCCTGG CGAGAGGGGC TTCGGGGGCT TGAAGGGCCA GAAAGGTGAT
    ACAATTTCTT GTAACGTCAC CTACCCTGGG AGGCCGGGGC CCCCAGGTTT TGATGGACCT
    CCAGGACCAA AGGGATTTCC AGGTCCTCAA GGCGCTCCAG GGTTGAGGTG CTTGGATGGG
    CCAAAAGGTC AGCGTGGCAA ACCAGGAATG TCAGAGATAC CCGGTCCACC TGGTTTTCGT
    GGTGACATGG GAGATCCGGG TTTTGAAGGT GAAAAGGGGT CCTCCCTTCT TGGGCCGCCA
    GGCTTTCCTG GTTCTCGTGG AGCAAATGGT CAGAAAGGAA TGATGGGAGA CATTGCCTAT
    GGCCTCCCAG GCCCCCCAGG AAAGAGAGGT CCTTCCGGAG TGCCAGGGTC CAAAGGACAC
    AGAGGTGATT CAGGACGTCC AGGGTTTGCA GGGCCAGCCG GCAAGCCTGG ATCCCCAGGT
    CTCAAAGGTC CCAGAGGCAG AGAGGGAAGT GCTGGGTTTC AAGGTATCCC GGGTCCACCT
    GGCCATTCCT GCAAAAGAGG CGCTCCAGGG ATACCAGGGC AACCGGGGCT CCCTGGGGCC
    CCGGGGAGTC CAGGTGCCCC AGGTTGGAAA GGACAGCGAG GGGATGTGGG GCCTCCGGGT
    CCAGCTGGAA TGAAAGGCCT CCCTGGAGTC CCTGGACGGC CAGGGGCAGA GGGACCCCTA
    GGATTCCCAG GAGTCCCAGG CCCCTTAGGG GATGATGGGC TGCCTGGTCT TCCAGGCCCG
    AAGGGACCCC AGGGGCTGCC TGGCTTCCCC GGTTTTCCAG GGGAGAGAGG AAAGCCTGGC
    CCAGAGGGAC GCCCTGGCAG CAAGGGGGAA CCAGGAGAGG ATGGTCGGCC TGGCTTCTTC
    GGAGACCAAG GGGTGAAAGG TGCCAAAGGA GCGAGAGGAC CCCCAGGAGA TGAAGGAGAG
    ATGGCGGTCT TTTCCCAAAA AGGGAAAACC GGGGAACCTG GACCTCCAGG AGATGATGGA
    TTCCCAGGAG AAGAAGGTGA TAAAGGCAAT CCAGGGATGC CGGGGAGGAG AGGAGAGCCT
    GGAAGATTCG GAGCACCTGG ATTTCACAGA GGGGAGCCCG GGAGAACCGG GCAGCCAGGG
    CTTCCTGGCC CCCCAGGCCC CCCAGGCTCA CCTGGGCTGA GAGGGATTAT TGGTTTTCCG
    GGATTTCCAG GTGACCAGGG TGAGCCAGGT TCTCCAGGGT CTCCTGGACT TTCAGGAATT
    GATGGAATGA GAGGACCTAA AGGAAGCAAA GGTGACCCTG CAAGTCAGTT CGGCCCACCT
    GGTCCAAAGG GTGAACCAGG TAGCCCTGGA TGTCCAGGAC AAGTTGGAGC ACCTGGAGAG
    CAGGGCTTGC CTGGTGTTCA AGGGCTCAGA GGACCACCTG GAAGGCCAGG ATTACCTGGC
    TCCTCCGGAC CACCAGGGTG TCCAGGTAAT CAAGGGGTGC CTGGGCTGAC AGGGCCTCCA
    GGAGAAACAG GGGATCCTGG GCCAAGAGGC ATGATGGGAG ATCCAGGGCC ACCAGGTCTT
    CCAGGAATAA AAGGTCCCTC CGGGTCGCCG GGTCTGAATG GCTTGCATGG TTTAAAGGGT
    CAGAAAGGAG CCAAAGGTGC TTCAGGTTTG CACGAAGTGG GCCCACCCGG TCCAGTGGGC
    GTACCTGGGC TGAAAGGGGA GCCGGGAGAC CCTGGGAGCC CAGGAATTTC TCCCCCAGGC
    CTTTCTGGAG AAAGAGGCCC CCCCGGCCCC CCAGGGAGAC CTGGAGCCCC TGGTCCTGCA
    GGTGCCACAG GAAGAGCTGC TGAAGGTCAC GTTCCTGACC CAGGTCCACC CGGAGATGTG
    GGTCCTCCTG GCCCCGATGG TCCGAGAGGA GCGCCCGGGC CCCCAGGCCC CCCCGGGAGC
    GTTGACCTTC TGAGAGGGGA ACCAGGAGAC TGTGGGCCGC CGGGGCCTCC AGGTCCCCCG
    GGCCCACCCG GCCCTCCAGG ACACCAAGGC TTCCCAGGAT GCGATGGAAA AGACGGCCAG
    AAAGGACCAA TAGGATTCCC GGGGCTGCAG GGGCCTCAGG GACTTCCTGG GCTCCCTGGG
    GAGAAGGGTC TGCTGGGCAT TCCAGGCCGG CGAGGGCACC CCGGTCCTCC AGGTTCCAGA
    GGTGAACCAG GGCCTCCTGC AGATGTGGAT TCGTGCCCCC GCATCCCCGG GCTTCCTGGG
    GTACCAGGCC CAAGAGGACC AGAAGGAGCC ATGGGGGTCC CTGGAAGGAG AGGGCCCCCA
    GGACCAGGGT GCAAAGGAGA GCCGGGACTG GAGGGCAGGA GGGGCGAGGC CGGCCTCCCA
    GGACCTCCGG GGCCTCCCGG AAGCACAGGG GACGCAGGAG AAGCCGGTTG CCCCGGAGCA
    CCAGGGCCCC CCGGGCCCAT TGGGGACCCT GGGCCCAGAG GGTTTGGGCC TGGATACCTC
    AGTGGCTTCC TCCTGGTTCT CCACAGTCAG ACGGATGGGG AGCCCACCTG CCCCGCGGGC
    ATGCCCAGGC TCTGGACGGG CTACAGTCTG TTATACCTGG AAGGACAGGA GAAGGCCCAC
    AATCAGGACC TTGGTCTGGC AGGGTCTTGC CTTCCCATGT TCAGCACCCT GCCCTTTGCC
    TACTGCAACA TCCACCAAGT GTGCCACTAC GCCCGGAGGA ACGACCGGTC CTACTGGCTG
    GCCAGTGCTG CGCCCCTGCC CATGATGCCG CTCTCAGAGG AGGAGATCCG CCCCTACATC
    AGCCGCTGTG CCGTGTGCGA GGCCCCAGCC CCCGTGGTGG CGCTGCACAG CCAGGACCGG
    TCCATCCCGC CGTGTCCACG GAGCTGGAGG AGCCTCTGGA TCGGGTACTC GTTCCTGATG
    CACACAGGGG CTGGAGACCA AGGAGGAGGG CAGGCCCTCA TGTCCCCCGG CAGCTGTCTG
    GAAGATTTCC GAGCCGCACC GTTCCTCGAA TGCCAAGGCC GACAGGGAAC TTGCCACTTT
    TTTGCAAATG AGTATAGCTT CTGGCTGACG ACGGTGAGGC CTGACTTGCA GTTTTCCTCG
    GCACCATCAC CAGACACCTT GAAAGAAAGC CAGGCCCAGC GCCAGAGAAT CAGCAGGTGC
    CAGGTCTGCA TGAAGTATGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297002
    Clone ID Related Accession (Same CDS sequence) XM_022409541.1
    Accession Version XM_022409541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-4(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAGGTG ACAGTGGCCC TCCTGGAGCA GCAGGTGACA AAGGTGACAA GGGTCCAACT 
    GGTGTTCCTG GATTTCCAGG GTTGGATGGC ATACCTGGCC ACCCGGGGCC TCCTGGATCC
    AGAGGCAAGC CTGGCATGCA TGGCTACAAT GGTTCACGAG GTGATCCAGG GTTTCCAGGA
    GAAAGAGGAG TTCCTGGCCC AGGAGGCCCC CCAGGCCTTC CTGGTGAAAG GGGAGAAAAA
    GGAAATTCAG TGTTCATTTT AGGTGCCATT AAAGGTATTC AGGGTGACAG GGGGGACCCA
    GGACCTCCTG GCTTGCCGGG ACCGAAGGGG GCACGAGGAC CGGCAGGCCC AAGGGGACAG
    CCAGGAGAGC CTGGGTTAAC AGGTGCTCCG GGCCATCCTG GGAGACCAGG CTTGAAGGGT
    AATCCTGGTG TGGGAGTAAA GGGGCAAATG GGAGACCCGG GTGAGGTTGG CCAGCAGGGT
    TCTCCTGGAC CCACCTTATT GGTACAGCCA CCTGATTCCT GTCTGTATAA AGGAGAAAAG
    GGCATAAAAG GAATGCCTGG TATGATTGGA CCTCCAGGAC TACCGGGACC CAAGGGAGAA
    CCTGGAATTG GACCAAAAGG AGAGAAGGGT ATTCCTGGGT TCTCAGGACC TCGGGGTGAT
    CCGGGTTCCT ATGGCTCTCC AGGTTTTCCA GGATTAAAGG GGAAACCAGG ACTGTTTGGA
    GATCCTGGAT CATTTGGATT TGTTGGCCCA AAGGGGGATC CTGGAGACCG TGGGTACCCA
    GGACCACCGG GTGTTCTGGT AACTCCATCT GTTCCACTCA AAGGCCCTCC AGGGGATCCG
    GGGCGCCCTG GCCACTATGG AGAAATGGGG TCCGTTGGAC CACCTGGTCC CCCTGGTCCC
    CCAGGTCCAC CAGGGGAAGA CTGTGCAGGC ATGATGGGAC CCCCTGGACC AAGAGGATTT
    CCTGGTCATC CAGGATTTCC AGGGGCAGCT GGTATCCCAG GGAGAGCGGA TTTAAGTCCA
    GGAAAACCAG GGAAACCGGG ACCTCCTGGG TTGCCCGGAG CACCAGGGCT GCAAGGACCC
    CCAGGATCAG ATGTTATCTA TTGTAGTGTT GGGCATCCTG GACCACAAGG GATAAAAGGC
    AAAATGGGTC CTCCAGGAAG AAGAGGCTCG AAAGGAGAAA AAGGAAATGC GGGGCTCTGT
    GCCTGTGAGC CTGGTCCCAT GGGCCCACCA GGCCCTCCAG GACTTCCTGG AAGGCAGGGT
    AGTAAGGGAG ACTTGGGGCT CCCTGGGTGG ATTGGAGAGA AAGGTCACCC AGGCCCTCCT
    GGTGCTGAAG GATCTCCAGG ATCACCAGGA AAACATGGTG CCTCAGGACC ACCTGGCAGC
    AAAGGAGAAA AGGGCGACAG GGTTGTATCA AGAGTGAAAG GGAAAAAAGG AGAAAGAGGT
    CCTGATGGGC TCCCAGGATT TCCAGGACAA CAGGGACAAA ATGGTCGGGA TGGACTTCCT
    GGAAAAAAGG GGGATCCAGG CCCCCCGGGG GGTCATGAGG ATGCAGCCCC AGGTGCTAAA
    GGGTCTCCTG GACCACCGGG GCCCCCAGGC AGAACAGGAC CCAGGGGACA ACCAGGTCTG
    GGATTTCCTG GTCCACCAGG AGAGAGAGGA CAACCAGGAG CTCCAGGCCG CCCTGGCGAG
    AGGGGCTTCG GGGGCTTGAA GGGCCAGAAA GGTGATACAA TTTCTTGTAA CGTCACCTAC
    CCTGGGAGGC CGGGGCCCCC AGGTTTTGAT GGACCTCCAG GACCAAAGGG ATTTCCAGGT
    CCTCAAGGCG CTCCAGGGTT GAGGTGCTTG GATGGGCCAA AAGGTCAGCG TGGCAAACCA
    GGAATGTCAG AGATACCCGG TCCACCTGGT TTTCGTGGTG ACATGGGAGA TCCGGGTTTT
    GAAGGTGAAA AGGGGTCCTC CCTTCTTGGG CCGCCAGGCT TTCCTGGTTC TCGTGGAGCA
    AATGGTCAGA AAGGAATGAT GGGAGACATT GCCTATGGCC TCCCAGGCCC CCCAGGAAAG
    AGAGGTCCTT CCGGAGTGCC AGGGTCCAAA GGACACAGAG GTGATTCAGG ACGTCCAGGG
    TTTGCAGGGC CAGCCGGCAA GCCTGGATCC CCAGGTCTCA AAGGTCCCAG AGGCAGAGAG
    GGAAGTGCTG GGTTTCAAGG TATCCCGGGT CCACCTGGCC ATTCCTGCAA AAGAGGCGCT
    CCAGGGATAC CAGGGCAACC GGGGCTCCCT GGGGCCCCGG GGAGTCCAGG TGCCCCAGGT
    TGGAAAGGAC AGCGAGGGGA TGTGGGGCCT CCGGGTCCAG CTGGAATGAA AGGCCTCCCT
    GGAGTCCCTG GACGGCCAGG GGCAGAGGGA CCCCTAGGAT TCCCAGGAGT CCCAGGCCCC
    TTAGGGGATG ATGGGCTGCC TGGTCTTCCA GGCCCGAAGG GACCCCAGGG GCTGCCTGGC
    TTCCCCGGTT TTCCAGGGGA GAGAGGAAAG CCTGGCCCAG AGGGACGCCC TGGCAGCAAG
    GGGGAACCAG GAGAGGATGG TCGGCCTGGC TTCTTCGGAG ACCAAGGGGT GAAAGGTGCC
    AAAGGAGCGA GAGGACCCCC AGGAGATGAA GGAGAGATGG CGGTCTTTTC CCAAAAAGGG
    AAAACCGGGG AACCTGGACC TCCAGGAGAT GATGGATTCC CAGGAGAAGA AGGTGATAAA
    GGCAATCCAG GGATGCCGGG GAGGAGAGGA GAGCCTGGAA GATTCGGAGC ACCTGGATTT
    CACAGAGGGG AGCCCGGGAG AACCGGGCAG CCAGGGCTTC CTGGCCCCCC AGGCCCCCCA
    GGCTCACCTG GGCTGAGAGG GATTATTGGT TTTCCGGGAT TTCCAGGTGA CCAGGGTGAG
    CCAGGTTCTC CAGGGTCTCC TGGACTTTCA GGAATTGATG GAATGAGAGG ACCTAAAGGA
    AGCAAAGGTG ACCCTGCAAG TCAGTTCGGC CCACCTGGTC CAAAGGGTGA ACCAGGTAGC
    CCTGGATGTC CAGGACAAGT TGGAGCACCT GGAGAGCAGG GCTTGCCTGG TGTTCAAGGG
    CTCAGAGGAC CACCTGGAAG GCCAGGATTA CCTGGCTCCT CCGGACCACC AGGGTGTCCA
    GGTAATCAAG GGGTGCCTGG GCTGACAGGG CCTCCAGGAG AAACAGGGGA TCCTGGGCCA
    AGAGGCATGA TGGGAGATCC AGGGCCACCA GGTCTTCCAG GAATAAAAGG TCCCTCCGGG
    TCGCCGGGTC TGAATGGCTT GCATGGTTTA AAGGGTCAGA AAGGAGCCAA AGGTGCTTCA
    GGTTTGCACG AAGTGGGCCC ACCCGGTCCA GTGGGCGTAC CTGGGCTGAA AGGGGAGCCG
    GGAGACCCTG GGAGCCCAGG AATTTCTCCC CCAGGCCTTT CTGGAGAAAG AGGCCCCCCC
    GGCCCCCCAG GGAGACCTGG AGCCCCTGGT CCTGCAGGTG CCACAGGAAG AGCTGCTGAA
    GGTCACGTTC CTGACCCAGG TCCACCCGGA GATGTGGGTC CTCCTGGCCC CGATGGTCCG
    AGAGGAGCGC CCGGGCCCCC AGGCCCCCCC GGGAGCGTTG ACCTTCTGAG AGGGGAACCA
    GGAGACTGTG GGCCGCCGGG GCCTCCAGGT CCCCCGGGCC CACCCGGCCC TCCAGGACAC
    CAAGGCTTCC CAGGATGCGA TGGAAAAGAC GGCCAGAAAG GACCAATAGG ATTCCCGGGG
    CTGCAGGGGC CTCAGGGACT TCCTGGGCTC CCTGGGGAGA AGGGTCTGCT GGGCATTCCA
    GGCCGGCGAG GGCACCCCGG TCCTCCAGGT TCCAGAGGTG AACCAGGGCC TCCTGCAGAT
    GTGGATTCGT GCCCCCGCAT CCCCGGGCTT CCTGGGGTAC CAGGCCCAAG AGGACCAGAA
    GGAGCCATGG GGGTCCCTGG AAGGAGAGGG CCCCCAGGAC CAGGGTGCAA AGGAGAGCCG
    GGACTGGAGG GCAGGAGGGG CGAGGCCGGC CTCCCAGGAC CTCCGGGGCC TCCCGGAAGC
    ACAGGGGACG CAGGAGAAGC CGGTTGCCCC GGAGCACCAG GGCCCCCCGG GCCCATTGGG
    GACCCTGGGC CCAGAGGGTT TGGGCCTGGA TACCTCAGTG GCTTCCTCCT GGTTCTCCAC
    AGTCAGACGG ATGGGGAGCC CACCTGCCCC GCGGGCATGC CCAGGCTCTG GACGGGCTAC
    AGTCTGTTAT ACCTGGAAGG ACAGGAGAAG GCCCACAATC AGGACCTTGG TCTGGCAGGG
    TCTTGCCTTC CCATGTTCAG CACCCTGCCC TTTGCCTACT GCAACATCCA CCAAGTGTGC
    CACTACGCCC GGAGGAACGA CCGGTCCTAC TGGCTGGCCA GTGCTGCGCC CCTGCCCATG
    ATGCCGCTCT CAGAGGAGGA GATCCGCCCC TACATCAGCC GCTGTGCCGT GTGCGAGGCC
    CCAGCCCCCG TGGTGGCGCT GCACAGCCAG GACCGGTCCA TCCCGCCGTG TCCACGGAGC
    TGGAGGAGCC TCTGGATCGG GTACTCGTTC CTGATGCACA CAGGGGCTGG AGACCAAGGA
    GGAGGGCAGG CCCTCATGTC CCCCGGCAGC TGTCTGGAAG ATTTCCGAGC CGCACCGTTC
    CTCGAATGCC AAGGCCGACA GGGAACTTGC CACTTTTTTG CAAATGAGTA TAGCTTCTGG
    CTGACGACGG TGAGGCCTGA CTTGCAGTTT TCCTCGGCAC CATCACCAGA CACCTTGAAA
    GAAAGCCAGG CCCAGCGCCA GAGAATCAGC AGGTGCCAGG TCTGCATGAA GTATGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265249.1
    CDS452..5251
    Translation

    Target ORF information:

    RefSeq Version XM_022409541.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAGGTG ACAGTGGCCC TCCTGGAGCA GCAGGTGACA AAGGTGACAA GGGTCCAACT 
    GGTGTTCCTG GATTTCCAGG GTTGGATGGC ATACCTGGCC ACCCGGGGCC TCCTGGATCC
    AGAGGCAAGC CTGGCATGCA TGGCTACAAT GGTTCACGAG GTGATCCAGG GTTTCCAGGA
    GAAAGAGGAG TTCCTGGCCC AGGAGGCCCC CCAGGCCTTC CTGGTGAAAG GGGAGAAAAA
    GGAAATTCAG TGTTCATTTT AGGTGCCATT AAAGGTATTC AGGGTGACAG GGGGGACCCA
    GGACCTCCTG GCTTGCCGGG ACCGAAGGGG GCACGAGGAC CGGCAGGCCC AAGGGGACAG
    CCAGGAGAGC CTGGGTTAAC AGGTGCTCCG GGCCATCCTG GGAGACCAGG CTTGAAGGGT
    AATCCTGGTG TGGGAGTAAA GGGGCAAATG GGAGACCCGG GTGAGGTTGG CCAGCAGGGT
    TCTCCTGGAC CCACCTTATT GGTACAGCCA CCTGATTCCT GTCTGTATAA AGGAGAAAAG
    GGCATAAAAG GAATGCCTGG TATGATTGGA CCTCCAGGAC TACCGGGACC CAAGGGAGAA
    CCTGGAATTG GACCAAAAGG AGAGAAGGGT ATTCCTGGGT TCTCAGGACC TCGGGGTGAT
    CCGGGTTCCT ATGGCTCTCC AGGTTTTCCA GGATTAAAGG GGAAACCAGG ACTGTTTGGA
    GATCCTGGAT CATTTGGATT TGTTGGCCCA AAGGGGGATC CTGGAGACCG TGGGTACCCA
    GGACCACCGG GTGTTCTGGT AACTCCATCT GTTCCACTCA AAGGCCCTCC AGGGGATCCG
    GGGCGCCCTG GCCACTATGG AGAAATGGGG TCCGTTGGAC CACCTGGTCC CCCTGGTCCC
    CCAGGTCCAC CAGGGGAAGA CTGTGCAGGC ATGATGGGAC CCCCTGGACC AAGAGGATTT
    CCTGGTCATC CAGGATTTCC AGGGGCAGCT GGTATCCCAG GGAGAGCGGA TTTAAGTCCA
    GGAAAACCAG GGAAACCGGG ACCTCCTGGG TTGCCCGGAG CACCAGGGCT GCAAGGACCC
    CCAGGATCAG ATGTTATCTA TTGTAGTGTT GGGCATCCTG GACCACAAGG GATAAAAGGC
    AAAATGGGTC CTCCAGGAAG AAGAGGCTCG AAAGGAGAAA AAGGAAATGC GGGGCTCTGT
    GCCTGTGAGC CTGGTCCCAT GGGCCCACCA GGCCCTCCAG GACTTCCTGG AAGGCAGGGT
    AGTAAGGGAG ACTTGGGGCT CCCTGGGTGG ATTGGAGAGA AAGGTCACCC AGGCCCTCCT
    GGTGCTGAAG GATCTCCAGG ATCACCAGGA AAACATGGTG CCTCAGGACC ACCTGGCAGC
    AAAGGAGAAA AGGGCGACAG GGTTGTATCA AGAGTGAAAG GGAAAAAAGG AGAAAGAGGT
    CCTGATGGGC TCCCAGGATT TCCAGGACAA CAGGGACAAA ATGGTCGGGA TGGACTTCCT
    GGAAAAAAGG GGGATCCAGG CCCCCCGGGG GGTCATGAGG ATGCAGCCCC AGGTGCTAAA
    GGGTCTCCTG GACCACCGGG GCCCCCAGGC AGAACAGGAC CCAGGGGACA ACCAGGTCTG
    GGATTTCCTG GTCCACCAGG AGAGAGAGGA CAACCAGGAG CTCCAGGCCG CCCTGGCGAG
    AGGGGCTTCG GGGGCTTGAA GGGCCAGAAA GGTGATACAA TTTCTTGTAA CGTCACCTAC
    CCTGGGAGGC CGGGGCCCCC AGGTTTTGAT GGACCTCCAG GACCAAAGGG ATTTCCAGGT
    CCTCAAGGCG CTCCAGGGTT GAGGTGCTTG GATGGGCCAA AAGGTCAGCG TGGCAAACCA
    GGAATGTCAG AGATACCCGG TCCACCTGGT TTTCGTGGTG ACATGGGAGA TCCGGGTTTT
    GAAGGTGAAA AGGGGTCCTC CCTTCTTGGG CCGCCAGGCT TTCCTGGTTC TCGTGGAGCA
    AATGGTCAGA AAGGAATGAT GGGAGACATT GCCTATGGCC TCCCAGGCCC CCCAGGAAAG
    AGAGGTCCTT CCGGAGTGCC AGGGTCCAAA GGACACAGAG GTGATTCAGG ACGTCCAGGG
    TTTGCAGGGC CAGCCGGCAA GCCTGGATCC CCAGGTCTCA AAGGTCCCAG AGGCAGAGAG
    GGAAGTGCTG GGTTTCAAGG TATCCCGGGT CCACCTGGCC ATTCCTGCAA AAGAGGCGCT
    CCAGGGATAC CAGGGCAACC GGGGCTCCCT GGGGCCCCGG GGAGTCCAGG TGCCCCAGGT
    TGGAAAGGAC AGCGAGGGGA TGTGGGGCCT CCGGGTCCAG CTGGAATGAA AGGCCTCCCT
    GGAGTCCCTG GACGGCCAGG GGCAGAGGGA CCCCTAGGAT TCCCAGGAGT CCCAGGCCCC
    TTAGGGGATG ATGGGCTGCC TGGTCTTCCA GGCCCGAAGG GACCCCAGGG GCTGCCTGGC
    TTCCCCGGTT TTCCAGGGGA GAGAGGAAAG CCTGGCCCAG AGGGACGCCC TGGCAGCAAG
    GGGGAACCAG GAGAGGATGG TCGGCCTGGC TTCTTCGGAG ACCAAGGGGT GAAAGGTGCC
    AAAGGAGCGA GAGGACCCCC AGGAGATGAA GGAGAGATGG CGGTCTTTTC CCAAAAAGGG
    AAAACCGGGG AACCTGGACC TCCAGGAGAT GATGGATTCC CAGGAGAAGA AGGTGATAAA
    GGCAATCCAG GGATGCCGGG GAGGAGAGGA GAGCCTGGAA GATTCGGAGC ACCTGGATTT
    CACAGAGGGG AGCCCGGGAG AACCGGGCAG CCAGGGCTTC CTGGCCCCCC AGGCCCCCCA
    GGCTCACCTG GGCTGAGAGG GATTATTGGT TTTCCGGGAT TTCCAGGTGA CCAGGGTGAG
    CCAGGTTCTC CAGGGTCTCC TGGACTTTCA GGAATTGATG GAATGAGAGG ACCTAAAGGA
    AGCAAAGGTG ACCCTGCAAG TCAGTTCGGC CCACCTGGTC CAAAGGGTGA ACCAGGTAGC
    CCTGGATGTC CAGGACAAGT TGGAGCACCT GGAGAGCAGG GCTTGCCTGG TGTTCAAGGG
    CTCAGAGGAC CACCTGGAAG GCCAGGATTA CCTGGCTCCT CCGGACCACC AGGGTGTCCA
    GGTAATCAAG GGGTGCCTGG GCTGACAGGG CCTCCAGGAG AAACAGGGGA TCCTGGGCCA
    AGAGGCATGA TGGGAGATCC AGGGCCACCA GGTCTTCCAG GAATAAAAGG TCCCTCCGGG
    TCGCCGGGTC TGAATGGCTT GCATGGTTTA AAGGGTCAGA AAGGAGCCAA AGGTGCTTCA
    GGTTTGCACG AAGTGGGCCC ACCCGGTCCA GTGGGCGTAC CTGGGCTGAA AGGGGAGCCG
    GGAGACCCTG GGAGCCCAGG AATTTCTCCC CCAGGCCTTT CTGGAGAAAG AGGCCCCCCC
    GGCCCCCCAG GGAGACCTGG AGCCCCTGGT CCTGCAGGTG CCACAGGAAG AGCTGCTGAA
    GGTCACGTTC CTGACCCAGG TCCACCCGGA GATGTGGGTC CTCCTGGCCC CGATGGTCCG
    AGAGGAGCGC CCGGGCCCCC AGGCCCCCCC GGGAGCGTTG ACCTTCTGAG AGGGGAACCA
    GGAGACTGTG GGCCGCCGGG GCCTCCAGGT CCCCCGGGCC CACCCGGCCC TCCAGGACAC
    CAAGGCTTCC CAGGATGCGA TGGAAAAGAC GGCCAGAAAG GACCAATAGG ATTCCCGGGG
    CTGCAGGGGC CTCAGGGACT TCCTGGGCTC CCTGGGGAGA AGGGTCTGCT GGGCATTCCA
    GGCCGGCGAG GGCACCCCGG TCCTCCAGGT TCCAGAGGTG AACCAGGGCC TCCTGCAGAT
    GTGGATTCGT GCCCCCGCAT CCCCGGGCTT CCTGGGGTAC CAGGCCCAAG AGGACCAGAA
    GGAGCCATGG GGGTCCCTGG AAGGAGAGGG CCCCCAGGAC CAGGGTGCAA AGGAGAGCCG
    GGACTGGAGG GCAGGAGGGG CGAGGCCGGC CTCCCAGGAC CTCCGGGGCC TCCCGGAAGC
    ACAGGGGACG CAGGAGAAGC CGGTTGCCCC GGAGCACCAG GGCCCCCCGG GCCCATTGGG
    GACCCTGGGC CCAGAGGGTT TGGGCCTGGA TACCTCAGTG GCTTCCTCCT GGTTCTCCAC
    AGTCAGACGG ATGGGGAGCC CACCTGCCCC GCGGGCATGC CCAGGCTCTG GACGGGCTAC
    AGTCTGTTAT ACCTGGAAGG ACAGGAGAAG GCCCACAATC AGGACCTTGG TCTGGCAGGG
    TCTTGCCTTC CCATGTTCAG CACCCTGCCC TTTGCCTACT GCAACATCCA CCAAGTGTGC
    CACTACGCCC GGAGGAACGA CCGGTCCTAC TGGCTGGCCA GTGCTGCGCC CCTGCCCATG
    ATGCCGCTCT CAGAGGAGGA GATCCGCCCC TACATCAGCC GCTGTGCCGT GTGCGAGGCC
    CCAGCCCCCG TGGTGGCGCT GCACAGCCAG GACCGGTCCA TCCCGCCGTG TCCACGGAGC
    TGGAGGAGCC TCTGGATCGG GTACTCGTTC CTGATGCACA CAGGGGCTGG AGACCAAGGA
    GGAGGGCAGG CCCTCATGTC CCCCGGCAGC TGTCTGGAAG ATTTCCGAGC CGCACCGTTC
    CTCGAATGCC AAGGCCGACA GGGAACTTGC CACTTTTTTG CAAATGAGTA TAGCTTCTGG
    CTGACGACGG TGAGGCCTGA CTTGCAGTTT TCCTCGGCAC CATCACCAGA CACCTTGAAA
    GAAAGCCAGG CCCAGCGCCA GAGAATCAGC AGGTGCCAGG TCTGCATGAA GTATGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.