KIT cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIT
Entrez Gene ID	403811
Full Name	KIT proto-oncogene receptor tyrosine kinase
Synonyms	c-KIT
General protein information	Preferred Names KIT proto-oncogene receptor tyrosine kinase Names mast/stem cell growth factor receptor Kit SCFR c-KIT gene1 proto-oncogene c-Kit proto-oncogene tyrosine-protein kinase Kit receptor tyrosine kinase c-kit tyrosine-protein kinase Kit
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005627970.3	XP_005628027.1	mast/stem cell growth factor receptor Kit isoform X3
XM_005627969.2	XP_005628026.1	mast/stem cell growth factor receptor Kit isoform X2
XM_005627968.2	XP_005628025.1	mast/stem cell growth factor receptor Kit isoform X1
NM_001003181.1	NP_001003181.1	mast/stem cell growth factor receptor Kit precursor
XM_005627970.2	XP_005628027.1	mast/stem cell growth factor receptor Kit isoform X3
XM_005627968.3	XP_005628025.1	mast/stem cell growth factor receptor Kit isoform X1
XM_005627969.3	XP_005628026.1	mast/stem cell growth factor receptor Kit isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIT	NP_000213.1
Bos taurus (cattle)	KIT	NP_001159956.1
Rattus norvegicus (Norway rat)	Kit	NP_071600.1
Pan troglodytes (chimpanzee)	KIT	XP_517285.3
Danio rerio (zebrafish)	kita	NP_571128.1
Danio rerio (zebrafish)	kitb	NP_001137390.1
Xenopus tropicalis (tropical clawed frog)	kit	NP_001135545.1
	KIT	XP_001089071.1
Gallus gallus (chicken)	KIT	NP_989692.1
Mus musculus (house mouse)	Kit	NP_001116205.1
Canis lupus familiaris (dog)	KIT	NP_001003181.1

Gene Ontology
Bibliography

Related articles in PubMed

A decrease in ubiquitination and resulting prolonged life-span of KIT underlies the KIT overexpression-mediated imatinib resistance of KIT mutation-driven canine mast cell tumor cells.
Kobayashi M, Kuroki S, Kurita S, Miyamoto R, Tani H, Tamura K, Bonkobara M
Oncology reports38(4)2543-2550(2017 Oct)

Genomic profiling of canine mast cell tumors identifies DNA copy number aberrations associated with KIT mutations and high histological grade.
Mochizuki H, Thomas R, Moroff S, Breen M
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology25(2)129-143(2017 Jun)

KTN0158, a Humanized Anti-KIT Monoclonal Antibody, Demonstrates Biologic Activity against both Normal and Malignant Canine Mast Cells.
London CA, Gardner HL, Rippy S, Post G, La Perle K, Crew L, Lopresti-Morrow L, Garton AJ, McMahon G, LaVallee TM, Gedrich R
Clinical cancer research : an official journal of the American Association for Cancer Research23(10)2565-2574(2017 May)

Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas.
Chen YC, Liao JW, Hsu WL, Chang SC
BMC veterinary research12(1)142(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the high incidence of GNSK-deletion c-kit in canine HSAs implicates KIT isoforms as possibly participating in the tumorigenesis of canine hemangiosarcomas.
Title: Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas.

data indicate that c-KIT exon 11 mutations occur frequently in canine gastrointestinal stromal tumours
Title: Analysis of c-KIT exon 11 mutations in canine gastrointestinal stromal tumours.

These findings indicate that canine gastrointestinal stromal, and possibly mast cell tumors,may be responsive to molecular-targeted therapy.
Title: Imatinib mesylate treatment in a dog with gastrointestinal stromal tumors with a c-kit mutation.

Ki67(-)/KIT(+) animals had a longer survival time than Ki67(+)/KIT(+) animals (p=0.03).
Title: Ki67/KIT double immunohistochemical staining in cutaneous mast cell tumors from Boxer dogs.

This is the first report demonstrating the tumorigenic potential of a mutation in the extracellular domain of KIT.
Title: A point mutation in the extracellular domain of KIT promotes tumorigenesis of mast cells via ligand-independent auto-dimerization.

The following KIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00298	NM_001003181.1 Latest version!	Canis lupus familiaris KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe16039	XM_005627968.3 Latest version!	Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe16040	XM_005627969.3 Latest version!	Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe16041	XM_005627970.3 Latest version!	Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OCe16041	XM_005627970.2	Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe16039	XM_005627968.2	Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe16040	XM_005627969.2	Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIT ( NM_001003181.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003181.1 Canis lupus familiaris KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.

Price & Availability↑

CloneID	OCe00298
Clone ID Related Accession (Same CDS sequence)	NM_001003181.1
Accession Version	NM_001003181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Canis lupus familiaris(dog)
Product	mast/stem cell growth factor receptor Kit precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF044249.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF044249.1, AY313776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCGGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAGAACAGCC AGCAGACTAA TGCACAGACA 
CAGAGTAATA GCTGGCATCA TGGTGACTTC AATTTTGAAC GTCAGGAAAA GTTGATTATC 
AGCTCAGCAA GAGTTAATGA TTCTGGAGTG TTCATGTGTT ACGCCAATAA TACTTTTGGA 
TCAGCAAATG TCACAACAAC CTTGGAAGTA GTAGATAAAG GATTCATTAA TATCTTCCCC 
ATGATGAGTA CTACAATATT TGTAAATGAT GGACAGAATG TGGATCTGAT TGTTGAATAT 
GAGGCATATC CCAAACCGGA GCACCAGCAG TGGATCTATA TGAACAGAAC CTTCACTGAT 
AAATGGGAAG ATTATCCCAA GTCTGACAAT GAAAGTAATA TCAGATATGT GAGTGAACTT 
CATCTAACCA GATTAAAAGG GAACGAAGGA GGCACTTACA CATTTCAAGT GTCCAATTCC 
GATGTCAATT CTTCGGTGAC ATTTAATGTT TATGTGAACA CAAAACCAGA AATCCTGACT 
CATGAAAGTC TCACGAATGG CATGCTCCAG TGTGTGGTTG CAGGATTCCC AGAGCCCGCA 
GTAGGTTGGT ATTTCTGTCC AGGAGCTGAG CAGAGATGTT CTGTCCCTAT TGGGCCAATG 
GATGTGCAGA TGCAAAACTC GTCTCTGTCA CCGTCTGGAA AACTAGTGGT TCAGAGTTCC 
ATCGATTATA GTGCCTTCAA GCACAATGGC ACAGTCGAGT GTAGGGCTTA CAACAATGTA 
GGCAGGAGTT CTGCCTTTTT TAACTTTGCA TTTAAAGAAC AAATCCATCC CCACACCCTG 
TTCACACCTT TGCTGATTGG CTTTGTGATC GCAGCTGGAA TGATGTGCAT TATCGTGATG 
ATTCTTACCT ACAAGTATCT ACAGAAACCC ATGTATGAAG TACAGTGGAA GGTTGTTGAG 
GAGATCAATG GAAACAATTA TGTTTACATA GACCCAACAC AGCTTCCTTA CGATCACAAA 
TGGGAGTTTC CCAGAAACAG GCTGAGCTTT GGGAAAACTT TGGGTGCTGG TGCCTTCGGG 
AAAGTGGTTG AAGCCACCGC ATATGGCCTG ATTAAGTCGG ATGCGGCCAT GACTGTTGCC 
GTTAAGATGC TCAAACCAAG TGCCCATTTA ACCGAACGAG AAGCCCTAAT GTCTGAGCTC 
AAAGTCTTGA GTTACCTCGG TAATCATATG AATATTGTGA ATCTTCTTGG AGCGTGCACC 
GTTGGAGGGC CCACCTTGGT CATTACAGAA TATTGTTGCT ATGGTGATCT TTTGAATTTT 
TTGCGAAGGA AACGTGATTC ATTTATTTGC TCAAAGCAGG AAGATCACGG AGAAGTGGCA 
CTTTATAAGA ACCTTCTGCA TTCAAAGGAG TCTTCCTGCA GTGACAGTAC TAATGAATAC 
ATGGACATGA AACCCGGCGT TTCTTACGTT GTGCCAACCA AGGCAGACAA AAGGAGATCT 
GCGAGAATAG GCTCATACAT AGAAAGGGAT GTGACTCCTG CCATCATGGA AGATGATGAG 
TTGGCTCTAG ATCTAGAGGA CTTGCTGAGC TTTTCTTACC AGGTGGCCAA GGGTATGGCA 
TTCCTGGCCT CGAAGAATTG TATTCACAGA GACTTGGCTG CTAGAAATAT CCTCCTTACT 
CATGGTCGAA TCACAAAGAT TTGTGATTTT GGTCTAGCCA GAGACATCAA GAATGATTCT 
AATTATGTGG TCAAAGGAAA CGCTCGGCTA CCTGTGAAGT GGATGGCCCC TGAGAGCATT 
TTCAACTGTG TGTACACATT TGAAAGTGAT GTCTGGTCCT ATGGGATTTT TCTGTGGGAG 
CTCTTCTCTT TAGGAAGCAG CCCCTACCCT GGGATGCCAG TCGATTCAAA GTTCTACAAG 
ATGATCAAGG AAGGGTTCCG GATGCTCAGC CCTGAGCATG CACCTGCTGA AATGTATGAC 
ATCATGAAGA CGTGCTGGGA TGCTGATCCC CTGAAAAGGC CGACGTTCAA GCAGATCGTG 
CAGCTAATTG AGAAGCAGAT TTCAGATAGC ACCAATCATA TTTATTCCAA CCTCGCGAAC 
TGCAGCCCCA ACCCAGAGCG CCCCGTGGTG GACCATTCCG TGCGGATCAA TTCCGTGGGC 
AGCAGCGCGT CTTCCACCCA GCCTCTGCTG GTACACGAAG ATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003181.1
CDS	29..2956
Translation	MRGARGAWDFLCVLLLLLLLGVRTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGQNVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVGWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	NM_001003181.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003181.1

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCGGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAGAACAGCC AGCAGACTAA TGCACAGACA 
CAGAGTAATA GCTGGCATCA TGGTGACTTC AATTTTGAAC GTCAGGAAAA GTTGATTATC 
AGCTCAGCAA GAGTTAATGA TTCTGGAGTG TTCATGTGTT ACGCCAATAA TACTTTTGGA 
TCAGCAAATG TCACAACAAC CTTGGAAGTA GTAGATAAAG GATTCATTAA TATCTTCCCC 
ATGATGAGTA CTACAATATT TGTAAATGAT GGACAGAATG TGGATCTGAT TGTTGAATAT 
GAGGCATATC CCAAACCGGA GCACCAGCAG TGGATCTATA TGAACAGAAC CTTCACTGAT 
AAATGGGAAG ATTATCCCAA GTCTGACAAT GAAAGTAATA TCAGATATGT GAGTGAACTT 
CATCTAACCA GATTAAAAGG GAACGAAGGA GGCACTTACA CATTTCAAGT GTCCAATTCC 
GATGTCAATT CTTCGGTGAC ATTTAATGTT TATGTGAACA CAAAACCAGA AATCCTGACT 
CATGAAAGTC TCACGAATGG CATGCTCCAG TGTGTGGTTG CAGGATTCCC AGAGCCCGCA 
GTAGGTTGGT ATTTCTGTCC AGGAGCTGAG CAGAGATGTT CTGTCCCTAT TGGGCCAATG 
GATGTGCAGA TGCAAAACTC GTCTCTGTCA CCGTCTGGAA AACTAGTGGT TCAGAGTTCC 
ATCGATTATA GTGCCTTCAA GCACAATGGC ACAGTCGAGT GTAGGGCTTA CAACAATGTA 
GGCAGGAGTT CTGCCTTTTT TAACTTTGCA TTTAAAGAAC AAATCCATCC CCACACCCTG 
TTCACACCTT TGCTGATTGG CTTTGTGATC GCAGCTGGAA TGATGTGCAT TATCGTGATG 
ATTCTTACCT ACAAGTATCT ACAGAAACCC ATGTATGAAG TACAGTGGAA GGTTGTTGAG 
GAGATCAATG GAAACAATTA TGTTTACATA GACCCAACAC AGCTTCCTTA CGATCACAAA 
TGGGAGTTTC CCAGAAACAG GCTGAGCTTT GGGAAAACTT TGGGTGCTGG TGCCTTCGGG 
AAAGTGGTTG AAGCCACCGC ATATGGCCTG ATTAAGTCGG ATGCGGCCAT GACTGTTGCC 
GTTAAGATGC TCAAACCAAG TGCCCATTTA ACCGAACGAG AAGCCCTAAT GTCTGAGCTC 
AAAGTCTTGA GTTACCTCGG TAATCATATG AATATTGTGA ATCTTCTTGG AGCGTGCACC 
GTTGGAGGGC CCACCTTGGT CATTACAGAA TATTGTTGCT ATGGTGATCT TTTGAATTTT 
TTGCGAAGGA AACGTGATTC ATTTATTTGC TCAAAGCAGG AAGATCACGG AGAAGTGGCA 
CTTTATAAGA ACCTTCTGCA TTCAAAGGAG TCTTCCTGCA GTGACAGTAC TAATGAATAC 
ATGGACATGA AACCCGGCGT TTCTTACGTT GTGCCAACCA AGGCAGACAA AAGGAGATCT 
GCGAGAATAG GCTCATACAT AGAAAGGGAT GTGACTCCTG CCATCATGGA AGATGATGAG 
TTGGCTCTAG ATCTAGAGGA CTTGCTGAGC TTTTCTTACC AGGTGGCCAA GGGTATGGCA 
TTCCTGGCCT CGAAGAATTG TATTCACAGA GACTTGGCTG CTAGAAATAT CCTCCTTACT 
CATGGTCGAA TCACAAAGAT TTGTGATTTT GGTCTAGCCA GAGACATCAA GAATGATTCT 
AATTATGTGG TCAAAGGAAA CGCTCGGCTA CCTGTGAAGT GGATGGCCCC TGAGAGCATT 
TTCAACTGTG TGTACACATT TGAAAGTGAT GTCTGGTCCT ATGGGATTTT TCTGTGGGAG 
CTCTTCTCTT TAGGAAGCAG CCCCTACCCT GGGATGCCAG TCGATTCAAA GTTCTACAAG 
ATGATCAAGG AAGGGTTCCG GATGCTCAGC CCTGAGCATG CACCTGCTGA AATGTATGAC 
ATCATGAAGA CGTGCTGGGA TGCTGATCCC CTGAAAAGGC CGACGTTCAA GCAGATCGTG 
CAGCTAATTG AGAAGCAGAT TTCAGATAGC ACCAATCATA TTTATTCCAA CCTCGCGAAC 
TGCAGCCCCA ACCCAGAGCG CCCCGTGGTG GACCATTCCG TGCGGATCAA TTCCGTGGGC 
AGCAGCGCGT CTTCCACCCA GCCTCTGCTG GTACACGAAG ATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005627968.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628025.1 Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe16039
Clone ID Related Accession (Same CDS sequence)	XM_005627968.2 , XM_005627968.3
Accession Version	XM_005627968.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mast/stem cell growth factor receptor Kit isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGCAGACTAA TGCACAGACA 
CAGAGTAATA GCTGGCATCA TGGTGACTTC AATTTTGAAC GTCAGGAAAA GTTGATTATC 
AGCTCAGCAA GAGTTAATGA TTCTGGAGTG TTCATGTGTT ACGCCAATAA TACTTTTGGA 
TCAGCAAATG TCACAACAAC CTTGGAAGTA GTAGATAAAG GATTCATTAA TATCTTCCCC 
ATGATGAGTA CTACAATATT TGTAAATGAT GGAGAGAATG TGGATCTGAT TGTTGAATAT 
GAGGCATATC CCAAACCGGA GCACCAGCAG TGGATCTATA TGAACAGAAC CTTCACTGAT 
AAATGGGAAG ATTATCCCAA GTCTGACAAT GAAAGTAATA TCAGATATGT GAGTGAACTT 
CATCTAACCA GATTAAAAGG GAACGAAGGA GGCACTTACA CATTTCAAGT GTCCAATTCC 
GATGTCAATT CTTCGGTGAC ATTTAATGTT TATGTGAACA CAAAACCAGA AATCCTGACT 
CATGAAAGTC TCACAAATGG CATGCTCCAG TGTGTGGTTG CAGGATTCCC AGAGCCCGCA 
GTAGATTGGT ATTTCTGTCC AGGAGCTGAG CAGAGATGTT CTGTCCCTAT TGGGCCAATG 
GATGTGCAGA TGCAAAACTC GTCTCTGTCA CCGTCTGGAA AACTAGTGGT TCAGAGTTCC 
ATCGATTATA GTGCCTTCAA GCACAATGGC ACAGTCGAGT GTAGGGCTTA CAACAATGTA 
GGCAGGAGTT CTGCCTTTTT TAACTTTGCA TTTAAAGGTA ACAGCAAAGA ACAAATCCAT 
CCCCACACCC TGTTCACACC TTTGCTGATT GGCTTTGTGA TCGCAGCTGG AATGATGTGC 
ATTATCGTGA TGATTCTTAC CTACAAGTAT CTACAGAAAC CCATGTATGA AGTACAGTGG 
AAGGTTGTTG AGGAGATCAA TGGAAACAAT TATGTTTACA TAGACCCAAC ACAGCTTCCT 
TACGATCACA AATGGGAGTT TCCCAGAAAC AGGCTGAGCT TTGGGAAAAC TTTGGGTGCT 
GGTGCCTTCG GGAAAGTGGT TGAAGCCACT GCATATGGCC TGATTAAGTC GGATGCGGCC 
ATGACTGTTG CCGTTAAGAT GCTCAAACCA AGTGCCCATT TAACCGAACG AGAAGCCCTA 
ATGTCTGAGC TCAAAGTCTT GAGTTACCTC GGTAATCATA TGAATATTGT GAATCTTCTT 
GGAGCGTGCA CCGTTGGAGG GCCCACCTTG GTCATTACTG AATATTGTTG CTATGGTGAT 
CTTTTGAATT TTTTGCGAAG GAAACGTGAT TCATTTATTT GCTCAAAGCA GGAAGATCAC 
GGAGAAGTGG CACTTTATAA GAACCTTCTG CATTCAAAGG AGTCTTCCTG CAGTGACAGT 
ACTAATGAAT ACATGGACAT GAAACCCGGC GTTTCTTACG TTGTGCCAAC CAAGGCAGAC 
AAAAGGAGAT CTGCGAGAAT AGGCTCATAC ATAGAAAGGG ATGTGACTCC TGCCATCATG 
GAAGATGATG AGTTGGCTCT AGATCTAGAG GACTTGCTGA GCTTTTCTTA CCAGGTGGCC 
AAGGGTATGG CATTCCTGGC CTCGAAGAAT TGTATTCACA GAGACTTGGC TGCTAGAAAT 
ATCCTCCTTA CTCATGGTCG AATCACAAAG ATTTGTGATT TTGGTCTAGC CAGAGACATC 
AAGAATGATT CTAATTATGT GGTCAAAGGA AACGCTCGGC TACCTGTGAA GTGGATGGCC 
CCTGAGAGCA TTTTCAACTG TGTGTACACA TTTGAAAGTG ATGTCTGGTC CTATGGGATT 
TTTCTGTGGG AGCTCTTCTC TTTAGGAAGC AGCCCCTACC CTGGGATGCC AGTCGATTCA 
AAGTTCTACA AGATGATCAA GGAAGGGTTC CGGATGCTCA GCCCTGAGCA TGCACCTGCT 
GAAATGTATG ACATCATGAA GACGTGCTGG GATGCTGATC CCCTGAAAAG GCCGACGTTC 
AAGCAGATCG TGCAGCTAAT TGAGAAGCAG ATTTCAGATA GCACCAATCA TATTTATTCC 
AACCTCGCGA ACTGCAGCCC CAACCCAGAG CGCCCCGTGG TGGACCATTC CGTGCGGATC 
AATTCCGTGG GCAGCAGCGC GTCTTCCACC CAGCCTCTGC TGGTACACGA AGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628025.1
CDS	8..2947
Translation	MRGARGAWDFLCVLLLLLLLGVQTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGENVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVDWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKGNSKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005627968.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627968.2

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGCAGACTAA TGCACAGACA 
CAGAGTAATA GCTGGCATCA TGGTGACTTC AATTTTGAAC GTCAGGAAAA GTTGATTATC 
AGCTCAGCAA GAGTTAATGA TTCTGGAGTG TTCATGTGTT ACGCCAATAA TACTTTTGGA 
TCAGCAAATG TCACAACAAC CTTGGAAGTA GTAGATAAAG GATTCATTAA TATCTTCCCC 
ATGATGAGTA CTACAATATT TGTAAATGAT GGAGAGAATG TGGATCTGAT TGTTGAATAT 
GAGGCATATC CCAAACCGGA GCACCAGCAG TGGATCTATA TGAACAGAAC CTTCACTGAT 
AAATGGGAAG ATTATCCCAA GTCTGACAAT GAAAGTAATA TCAGATATGT GAGTGAACTT 
CATCTAACCA GATTAAAAGG GAACGAAGGA GGCACTTACA CATTTCAAGT GTCCAATTCC 
GATGTCAATT CTTCGGTGAC ATTTAATGTT TATGTGAACA CAAAACCAGA AATCCTGACT 
CATGAAAGTC TCACAAATGG CATGCTCCAG TGTGTGGTTG CAGGATTCCC AGAGCCCGCA 
GTAGATTGGT ATTTCTGTCC AGGAGCTGAG CAGAGATGTT CTGTCCCTAT TGGGCCAATG 
GATGTGCAGA TGCAAAACTC GTCTCTGTCA CCGTCTGGAA AACTAGTGGT TCAGAGTTCC 
ATCGATTATA GTGCCTTCAA GCACAATGGC ACAGTCGAGT GTAGGGCTTA CAACAATGTA 
GGCAGGAGTT CTGCCTTTTT TAACTTTGCA TTTAAAGGTA ACAGCAAAGA ACAAATCCAT 
CCCCACACCC TGTTCACACC TTTGCTGATT GGCTTTGTGA TCGCAGCTGG AATGATGTGC 
ATTATCGTGA TGATTCTTAC CTACAAGTAT CTACAGAAAC CCATGTATGA AGTACAGTGG 
AAGGTTGTTG AGGAGATCAA TGGAAACAAT TATGTTTACA TAGACCCAAC ACAGCTTCCT 
TACGATCACA AATGGGAGTT TCCCAGAAAC AGGCTGAGCT TTGGGAAAAC TTTGGGTGCT 
GGTGCCTTCG GGAAAGTGGT TGAAGCCACT GCATATGGCC TGATTAAGTC GGATGCGGCC 
ATGACTGTTG CCGTTAAGAT GCTCAAACCA AGTGCCCATT TAACCGAACG AGAAGCCCTA 
ATGTCTGAGC TCAAAGTCTT GAGTTACCTC GGTAATCATA TGAATATTGT GAATCTTCTT 
GGAGCGTGCA CCGTTGGAGG GCCCACCTTG GTCATTACTG AATATTGTTG CTATGGTGAT 
CTTTTGAATT TTTTGCGAAG GAAACGTGAT TCATTTATTT GCTCAAAGCA GGAAGATCAC 
GGAGAAGTGG CACTTTATAA GAACCTTCTG CATTCAAAGG AGTCTTCCTG CAGTGACAGT 
ACTAATGAAT ACATGGACAT GAAACCCGGC GTTTCTTACG TTGTGCCAAC CAAGGCAGAC 
AAAAGGAGAT CTGCGAGAAT AGGCTCATAC ATAGAAAGGG ATGTGACTCC TGCCATCATG 
GAAGATGATG AGTTGGCTCT AGATCTAGAG GACTTGCTGA GCTTTTCTTA CCAGGTGGCC 
AAGGGTATGG CATTCCTGGC CTCGAAGAAT TGTATTCACA GAGACTTGGC TGCTAGAAAT 
ATCCTCCTTA CTCATGGTCG AATCACAAAG ATTTGTGATT TTGGTCTAGC CAGAGACATC 
AAGAATGATT CTAATTATGT GGTCAAAGGA AACGCTCGGC TACCTGTGAA GTGGATGGCC 
CCTGAGAGCA TTTTCAACTG TGTGTACACA TTTGAAAGTG ATGTCTGGTC CTATGGGATT 
TTTCTGTGGG AGCTCTTCTC TTTAGGAAGC AGCCCCTACC CTGGGATGCC AGTCGATTCA 
AAGTTCTACA AGATGATCAA GGAAGGGTTC CGGATGCTCA GCCCTGAGCA TGCACCTGCT 
GAAATGTATG ACATCATGAA GACGTGCTGG GATGCTGATC CCCTGAAAAG GCCGACGTTC 
AAGCAGATCG TGCAGCTAAT TGAGAAGCAG ATTTCAGATA GCACCAATCA TATTTATTCC 
AACCTCGCGA ACTGCAGCCC CAACCCAGAG CGCCCCGTGG TGGACCATTC CGTGCGGATC 
AATTCCGTGG GCAGCAGCGC GTCTTCCACC CAGCCTCTGC TGGTACACGA AGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005627968.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628025.1 Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe16039
Clone ID Related Accession (Same CDS sequence)	XM_005627968.2 , XM_005627968.3
Accession Version	XM_005627968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mast/stem cell growth factor receptor Kit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGCAGACTAA TGCACAGACA 
CAGAGTAATA GCTGGCATCA TGGTGACTTC AATTTTGAAC GTCAGGAAAA GTTGATTATC 
AGCTCAGCAA GAGTTAATGA TTCTGGAGTG TTCATGTGTT ACGCCAATAA TACTTTTGGA 
TCAGCAAATG TCACAACAAC CTTGGAAGTA GTAGATAAAG GATTCATTAA TATCTTCCCC 
ATGATGAGTA CTACAATATT TGTAAATGAT GGAGAGAATG TGGATCTGAT TGTTGAATAT 
GAGGCATATC CCAAACCGGA GCACCAGCAG TGGATCTATA TGAACAGAAC CTTCACTGAT 
AAATGGGAAG ATTATCCCAA GTCTGACAAT GAAAGTAATA TCAGATATGT GAGTGAACTT 
CATCTAACCA GATTAAAAGG GAACGAAGGA GGCACTTACA CATTTCAAGT GTCCAATTCC 
GATGTCAATT CTTCGGTGAC ATTTAATGTT TATGTGAACA CAAAACCAGA AATCCTGACT 
CATGAAAGTC TCACAAATGG CATGCTCCAG TGTGTGGTTG CAGGATTCCC AGAGCCCGCA 
GTAGATTGGT ATTTCTGTCC AGGAGCTGAG CAGAGATGTT CTGTCCCTAT TGGGCCAATG 
GATGTGCAGA TGCAAAACTC GTCTCTGTCA CCGTCTGGAA AACTAGTGGT TCAGAGTTCC 
ATCGATTATA GTGCCTTCAA GCACAATGGC ACAGTCGAGT GTAGGGCTTA CAACAATGTA 
GGCAGGAGTT CTGCCTTTTT TAACTTTGCA TTTAAAGGTA ACAGCAAAGA ACAAATCCAT 
CCCCACACCC TGTTCACACC TTTGCTGATT GGCTTTGTGA TCGCAGCTGG AATGATGTGC 
ATTATCGTGA TGATTCTTAC CTACAAGTAT CTACAGAAAC CCATGTATGA AGTACAGTGG 
AAGGTTGTTG AGGAGATCAA TGGAAACAAT TATGTTTACA TAGACCCAAC ACAGCTTCCT 
TACGATCACA AATGGGAGTT TCCCAGAAAC AGGCTGAGCT TTGGGAAAAC TTTGGGTGCT 
GGTGCCTTCG GGAAAGTGGT TGAAGCCACT GCATATGGCC TGATTAAGTC GGATGCGGCC 
ATGACTGTTG CCGTTAAGAT GCTCAAACCA AGTGCCCATT TAACCGAACG AGAAGCCCTA 
ATGTCTGAGC TCAAAGTCTT GAGTTACCTC GGTAATCATA TGAATATTGT GAATCTTCTT 
GGAGCGTGCA CCGTTGGAGG GCCCACCTTG GTCATTACTG AATATTGTTG CTATGGTGAT 
CTTTTGAATT TTTTGCGAAG GAAACGTGAT TCATTTATTT GCTCAAAGCA GGAAGATCAC 
GGAGAAGTGG CACTTTATAA GAACCTTCTG CATTCAAAGG AGTCTTCCTG CAGTGACAGT 
ACTAATGAAT ACATGGACAT GAAACCCGGC GTTTCTTACG TTGTGCCAAC CAAGGCAGAC 
AAAAGGAGAT CTGCGAGAAT AGGCTCATAC ATAGAAAGGG ATGTGACTCC TGCCATCATG 
GAAGATGATG AGTTGGCTCT AGATCTAGAG GACTTGCTGA GCTTTTCTTA CCAGGTGGCC 
AAGGGTATGG CATTCCTGGC CTCGAAGAAT TGTATTCACA GAGACTTGGC TGCTAGAAAT 
ATCCTCCTTA CTCATGGTCG AATCACAAAG ATTTGTGATT TTGGTCTAGC CAGAGACATC 
AAGAATGATT CTAATTATGT GGTCAAAGGA AACGCTCGGC TACCTGTGAA GTGGATGGCC 
CCTGAGAGCA TTTTCAACTG TGTGTACACA TTTGAAAGTG ATGTCTGGTC CTATGGGATT 
TTTCTGTGGG AGCTCTTCTC TTTAGGAAGC AGCCCCTACC CTGGGATGCC AGTCGATTCA 
AAGTTCTACA AGATGATCAA GGAAGGGTTC CGGATGCTCA GCCCTGAGCA TGCACCTGCT 
GAAATGTATG ACATCATGAA GACGTGCTGG GATGCTGATC CCCTGAAAAG GCCGACGTTC 
AAGCAGATCG TGCAGCTAAT TGAGAAGCAG ATTTCAGATA GCACCAATCA TATTTATTCC 
AACCTCGCGA ACTGCAGCCC CAACCCAGAG CGCCCCGTGG TGGACCATTC CGTGCGGATC 
AATTCCGTGG GCAGCAGCGC GTCTTCCACC CAGCCTCTGC TGGTACACGA AGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628025.1
CDS	91..3030
Translation	MRGARGAWDFLCVLLLLLLLGVQTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGENVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVDWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKGNSKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005627968.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627968.3

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGCAGACTAA TGCACAGACA 
CAGAGTAATA GCTGGCATCA TGGTGACTTC AATTTTGAAC GTCAGGAAAA GTTGATTATC 
AGCTCAGCAA GAGTTAATGA TTCTGGAGTG TTCATGTGTT ACGCCAATAA TACTTTTGGA 
TCAGCAAATG TCACAACAAC CTTGGAAGTA GTAGATAAAG GATTCATTAA TATCTTCCCC 
ATGATGAGTA CTACAATATT TGTAAATGAT GGAGAGAATG TGGATCTGAT TGTTGAATAT 
GAGGCATATC CCAAACCGGA GCACCAGCAG TGGATCTATA TGAACAGAAC CTTCACTGAT 
AAATGGGAAG ATTATCCCAA GTCTGACAAT GAAAGTAATA TCAGATATGT GAGTGAACTT 
CATCTAACCA GATTAAAAGG GAACGAAGGA GGCACTTACA CATTTCAAGT GTCCAATTCC 
GATGTCAATT CTTCGGTGAC ATTTAATGTT TATGTGAACA CAAAACCAGA AATCCTGACT 
CATGAAAGTC TCACAAATGG CATGCTCCAG TGTGTGGTTG CAGGATTCCC AGAGCCCGCA 
GTAGATTGGT ATTTCTGTCC AGGAGCTGAG CAGAGATGTT CTGTCCCTAT TGGGCCAATG 
GATGTGCAGA TGCAAAACTC GTCTCTGTCA CCGTCTGGAA AACTAGTGGT TCAGAGTTCC 
ATCGATTATA GTGCCTTCAA GCACAATGGC ACAGTCGAGT GTAGGGCTTA CAACAATGTA 
GGCAGGAGTT CTGCCTTTTT TAACTTTGCA TTTAAAGGTA ACAGCAAAGA ACAAATCCAT 
CCCCACACCC TGTTCACACC TTTGCTGATT GGCTTTGTGA TCGCAGCTGG AATGATGTGC 
ATTATCGTGA TGATTCTTAC CTACAAGTAT CTACAGAAAC CCATGTATGA AGTACAGTGG 
AAGGTTGTTG AGGAGATCAA TGGAAACAAT TATGTTTACA TAGACCCAAC ACAGCTTCCT 
TACGATCACA AATGGGAGTT TCCCAGAAAC AGGCTGAGCT TTGGGAAAAC TTTGGGTGCT 
GGTGCCTTCG GGAAAGTGGT TGAAGCCACT GCATATGGCC TGATTAAGTC GGATGCGGCC 
ATGACTGTTG CCGTTAAGAT GCTCAAACCA AGTGCCCATT TAACCGAACG AGAAGCCCTA 
ATGTCTGAGC TCAAAGTCTT GAGTTACCTC GGTAATCATA TGAATATTGT GAATCTTCTT 
GGAGCGTGCA CCGTTGGAGG GCCCACCTTG GTCATTACTG AATATTGTTG CTATGGTGAT 
CTTTTGAATT TTTTGCGAAG GAAACGTGAT TCATTTATTT GCTCAAAGCA GGAAGATCAC 
GGAGAAGTGG CACTTTATAA GAACCTTCTG CATTCAAAGG AGTCTTCCTG CAGTGACAGT 
ACTAATGAAT ACATGGACAT GAAACCCGGC GTTTCTTACG TTGTGCCAAC CAAGGCAGAC 
AAAAGGAGAT CTGCGAGAAT AGGCTCATAC ATAGAAAGGG ATGTGACTCC TGCCATCATG 
GAAGATGATG AGTTGGCTCT AGATCTAGAG GACTTGCTGA GCTTTTCTTA CCAGGTGGCC 
AAGGGTATGG CATTCCTGGC CTCGAAGAAT TGTATTCACA GAGACTTGGC TGCTAGAAAT 
ATCCTCCTTA CTCATGGTCG AATCACAAAG ATTTGTGATT TTGGTCTAGC CAGAGACATC 
AAGAATGATT CTAATTATGT GGTCAAAGGA AACGCTCGGC TACCTGTGAA GTGGATGGCC 
CCTGAGAGCA TTTTCAACTG TGTGTACACA TTTGAAAGTG ATGTCTGGTC CTATGGGATT 
TTTCTGTGGG AGCTCTTCTC TTTAGGAAGC AGCCCCTACC CTGGGATGCC AGTCGATTCA 
AAGTTCTACA AGATGATCAA GGAAGGGTTC CGGATGCTCA GCCCTGAGCA TGCACCTGCT 
GAAATGTATG ACATCATGAA GACGTGCTGG GATGCTGATC CCCTGAAAAG GCCGACGTTC 
AAGCAGATCG TGCAGCTAAT TGAGAAGCAG ATTTCAGATA GCACCAATCA TATTTATTCC 
AACCTCGCGA ACTGCAGCCC CAACCCAGAG CGCCCCGTGG TGGACCATTC CGTGCGGATC 
AATTCCGTGG GCAGCAGCGC GTCTTCCACC CAGCCTCTGC TGGTACACGA AGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005627969.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628026.1 Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe16040
Clone ID Related Accession (Same CDS sequence)	XM_005627969.2 , XM_005627969.3
Accession Version	XM_005627969.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mast/stem cell growth factor receptor Kit isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAACA AATCCATCCC 
CACACCCTGT TCACACCTTT GCTGATTGGC TTTGTGATCG CAGCTGGAAT GATGTGCATT 
ATCGTGATGA TTCTTACCTA CAAGTATCTA CAGAAACCCA TGTATGAAGT ACAGTGGAAG 
GTTGTTGAGG AGATCAATGG AAACAATTAT GTTTACATAG ACCCAACACA GCTTCCTTAC 
GATCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGCTTTG GGAAAACTTT GGGTGCTGGT 
GCCTTCGGGA AAGTGGTTGA AGCCACTGCA TATGGCCTGA TTAAGTCGGA TGCGGCCATG 
ACTGTTGCCG TTAAGATGCT CAAACCAAGT GCCCATTTAA CCGAACGAGA AGCCCTAATG 
TCTGAGCTCA AAGTCTTGAG TTACCTCGGT AATCATATGA ATATTGTGAA TCTTCTTGGA 
GCGTGCACCG TTGGAGGGCC CACCTTGGTC ATTACTGAAT ATTGTTGCTA TGGTGATCTT 
TTGAATTTTT TGCGAAGGAA ACGTGATTCA TTTATTTGCT CAAAGCAGGA AGATCACGGA 
GAAGTGGCAC TTTATAAGAA CCTTCTGCAT TCAAAGGAGT CTTCCTGCAG TGACAGTACT 
AATGAATACA TGGACATGAA ACCCGGCGTT TCTTACGTTG TGCCAACCAA GGCAGACAAA 
AGGAGATCTG CGAGAATAGG CTCATACATA GAAAGGGATG TGACTCCTGC CATCATGGAA 
GATGATGAGT TGGCTCTAGA TCTAGAGGAC TTGCTGAGCT TTTCTTACCA GGTGGCCAAG 
GGTATGGCAT TCCTGGCCTC GAAGAATTGT ATTCACAGAG ACTTGGCTGC TAGAAATATC 
CTCCTTACTC ATGGTCGAAT CACAAAGATT TGTGATTTTG GTCTAGCCAG AGACATCAAG 
AATGATTCTA ATTATGTGGT CAAAGGAAAC GCTCGGCTAC CTGTGAAGTG GATGGCCCCT 
GAGAGCATTT TCAACTGTGT GTACACATTT GAAAGTGATG TCTGGTCCTA TGGGATTTTT 
CTGTGGGAGC TCTTCTCTTT AGGAAGCAGC CCCTACCCTG GGATGCCAGT CGATTCAAAG 
TTCTACAAGA TGATCAAGGA AGGGTTCCGG ATGCTCAGCC CTGAGCATGC ACCTGCTGAA 
ATGTATGACA TCATGAAGAC GTGCTGGGAT GCTGATCCCC TGAAAAGGCC GACGTTCAAG 
CAGATCGTGC AGCTAATTGA GAAGCAGATT TCAGATAGCA CCAATCATAT TTATTCCAAC 
CTCGCGAACT GCAGCCCCAA CCCAGAGCGC CCCGTGGTGG ACCATTCCGT GCGGATCAAT 
TCCGTGGGCA GCAGCGCGTC TTCCACCCAG CCTCTGCTGG TACACGAAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628026.1
CDS	125..3061
Translation	MRGARGAWDFLCVLLLLLLLGVQTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGENVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVDWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKGNSKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005627969.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627969.2

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAACA AATCCATCCC 
CACACCCTGT TCACACCTTT GCTGATTGGC TTTGTGATCG CAGCTGGAAT GATGTGCATT 
ATCGTGATGA TTCTTACCTA CAAGTATCTA CAGAAACCCA TGTATGAAGT ACAGTGGAAG 
GTTGTTGAGG AGATCAATGG AAACAATTAT GTTTACATAG ACCCAACACA GCTTCCTTAC 
GATCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGCTTTG GGAAAACTTT GGGTGCTGGT 
GCCTTCGGGA AAGTGGTTGA AGCCACTGCA TATGGCCTGA TTAAGTCGGA TGCGGCCATG 
ACTGTTGCCG TTAAGATGCT CAAACCAAGT GCCCATTTAA CCGAACGAGA AGCCCTAATG 
TCTGAGCTCA AAGTCTTGAG TTACCTCGGT AATCATATGA ATATTGTGAA TCTTCTTGGA 
GCGTGCACCG TTGGAGGGCC CACCTTGGTC ATTACTGAAT ATTGTTGCTA TGGTGATCTT 
TTGAATTTTT TGCGAAGGAA ACGTGATTCA TTTATTTGCT CAAAGCAGGA AGATCACGGA 
GAAGTGGCAC TTTATAAGAA CCTTCTGCAT TCAAAGGAGT CTTCCTGCAG TGACAGTACT 
AATGAATACA TGGACATGAA ACCCGGCGTT TCTTACGTTG TGCCAACCAA GGCAGACAAA 
AGGAGATCTG CGAGAATAGG CTCATACATA GAAAGGGATG TGACTCCTGC CATCATGGAA 
GATGATGAGT TGGCTCTAGA TCTAGAGGAC TTGCTGAGCT TTTCTTACCA GGTGGCCAAG 
GGTATGGCAT TCCTGGCCTC GAAGAATTGT ATTCACAGAG ACTTGGCTGC TAGAAATATC 
CTCCTTACTC ATGGTCGAAT CACAAAGATT TGTGATTTTG GTCTAGCCAG AGACATCAAG 
AATGATTCTA ATTATGTGGT CAAAGGAAAC GCTCGGCTAC CTGTGAAGTG GATGGCCCCT 
GAGAGCATTT TCAACTGTGT GTACACATTT GAAAGTGATG TCTGGTCCTA TGGGATTTTT 
CTGTGGGAGC TCTTCTCTTT AGGAAGCAGC CCCTACCCTG GGATGCCAGT CGATTCAAAG 
TTCTACAAGA TGATCAAGGA AGGGTTCCGG ATGCTCAGCC CTGAGCATGC ACCTGCTGAA 
ATGTATGACA TCATGAAGAC GTGCTGGGAT GCTGATCCCC TGAAAAGGCC GACGTTCAAG 
CAGATCGTGC AGCTAATTGA GAAGCAGATT TCAGATAGCA CCAATCATAT TTATTCCAAC 
CTCGCGAACT GCAGCCCCAA CCCAGAGCGC CCCGTGGTGG ACCATTCCGT GCGGATCAAT 
TCCGTGGGCA GCAGCGCGTC TTCCACCCAG CCTCTGCTGG TACACGAAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005627969.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628026.1 Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe16040
Clone ID Related Accession (Same CDS sequence)	XM_005627969.2 , XM_005627969.3
Accession Version	XM_005627969.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mast/stem cell growth factor receptor Kit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAACA AATCCATCCC 
CACACCCTGT TCACACCTTT GCTGATTGGC TTTGTGATCG CAGCTGGAAT GATGTGCATT 
ATCGTGATGA TTCTTACCTA CAAGTATCTA CAGAAACCCA TGTATGAAGT ACAGTGGAAG 
GTTGTTGAGG AGATCAATGG AAACAATTAT GTTTACATAG ACCCAACACA GCTTCCTTAC 
GATCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGCTTTG GGAAAACTTT GGGTGCTGGT 
GCCTTCGGGA AAGTGGTTGA AGCCACTGCA TATGGCCTGA TTAAGTCGGA TGCGGCCATG 
ACTGTTGCCG TTAAGATGCT CAAACCAAGT GCCCATTTAA CCGAACGAGA AGCCCTAATG 
TCTGAGCTCA AAGTCTTGAG TTACCTCGGT AATCATATGA ATATTGTGAA TCTTCTTGGA 
GCGTGCACCG TTGGAGGGCC CACCTTGGTC ATTACTGAAT ATTGTTGCTA TGGTGATCTT 
TTGAATTTTT TGCGAAGGAA ACGTGATTCA TTTATTTGCT CAAAGCAGGA AGATCACGGA 
GAAGTGGCAC TTTATAAGAA CCTTCTGCAT TCAAAGGAGT CTTCCTGCAG TGACAGTACT 
AATGAATACA TGGACATGAA ACCCGGCGTT TCTTACGTTG TGCCAACCAA GGCAGACAAA 
AGGAGATCTG CGAGAATAGG CTCATACATA GAAAGGGATG TGACTCCTGC CATCATGGAA 
GATGATGAGT TGGCTCTAGA TCTAGAGGAC TTGCTGAGCT TTTCTTACCA GGTGGCCAAG 
GGTATGGCAT TCCTGGCCTC GAAGAATTGT ATTCACAGAG ACTTGGCTGC TAGAAATATC 
CTCCTTACTC ATGGTCGAAT CACAAAGATT TGTGATTTTG GTCTAGCCAG AGACATCAAG 
AATGATTCTA ATTATGTGGT CAAAGGAAAC GCTCGGCTAC CTGTGAAGTG GATGGCCCCT 
GAGAGCATTT TCAACTGTGT GTACACATTT GAAAGTGATG TCTGGTCCTA TGGGATTTTT 
CTGTGGGAGC TCTTCTCTTT AGGAAGCAGC CCCTACCCTG GGATGCCAGT CGATTCAAAG 
TTCTACAAGA TGATCAAGGA AGGGTTCCGG ATGCTCAGCC CTGAGCATGC ACCTGCTGAA 
ATGTATGACA TCATGAAGAC GTGCTGGGAT GCTGATCCCC TGAAAAGGCC GACGTTCAAG 
CAGATCGTGC AGCTAATTGA GAAGCAGATT TCAGATAGCA CCAATCATAT TTATTCCAAC 
CTCGCGAACT GCAGCCCCAA CCCAGAGCGC CCCGTGGTGG ACCATTCCGT GCGGATCAAT 
TCCGTGGGCA GCAGCGCGTC TTCCACCCAG CCTCTGCTGG TACACGAAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628026.1
CDS	91..3027
Translation	MRGARGAWDFLCVLLLLLLLGVQTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGENVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVDWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKGNSKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005627969.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627969.3

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAACA AATCCATCCC 
CACACCCTGT TCACACCTTT GCTGATTGGC TTTGTGATCG CAGCTGGAAT GATGTGCATT 
ATCGTGATGA TTCTTACCTA CAAGTATCTA CAGAAACCCA TGTATGAAGT ACAGTGGAAG 
GTTGTTGAGG AGATCAATGG AAACAATTAT GTTTACATAG ACCCAACACA GCTTCCTTAC 
GATCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGCTTTG GGAAAACTTT GGGTGCTGGT 
GCCTTCGGGA AAGTGGTTGA AGCCACTGCA TATGGCCTGA TTAAGTCGGA TGCGGCCATG 
ACTGTTGCCG TTAAGATGCT CAAACCAAGT GCCCATTTAA CCGAACGAGA AGCCCTAATG 
TCTGAGCTCA AAGTCTTGAG TTACCTCGGT AATCATATGA ATATTGTGAA TCTTCTTGGA 
GCGTGCACCG TTGGAGGGCC CACCTTGGTC ATTACTGAAT ATTGTTGCTA TGGTGATCTT 
TTGAATTTTT TGCGAAGGAA ACGTGATTCA TTTATTTGCT CAAAGCAGGA AGATCACGGA 
GAAGTGGCAC TTTATAAGAA CCTTCTGCAT TCAAAGGAGT CTTCCTGCAG TGACAGTACT 
AATGAATACA TGGACATGAA ACCCGGCGTT TCTTACGTTG TGCCAACCAA GGCAGACAAA 
AGGAGATCTG CGAGAATAGG CTCATACATA GAAAGGGATG TGACTCCTGC CATCATGGAA 
GATGATGAGT TGGCTCTAGA TCTAGAGGAC TTGCTGAGCT TTTCTTACCA GGTGGCCAAG 
GGTATGGCAT TCCTGGCCTC GAAGAATTGT ATTCACAGAG ACTTGGCTGC TAGAAATATC 
CTCCTTACTC ATGGTCGAAT CACAAAGATT TGTGATTTTG GTCTAGCCAG AGACATCAAG 
AATGATTCTA ATTATGTGGT CAAAGGAAAC GCTCGGCTAC CTGTGAAGTG GATGGCCCCT 
GAGAGCATTT TCAACTGTGT GTACACATTT GAAAGTGATG TCTGGTCCTA TGGGATTTTT 
CTGTGGGAGC TCTTCTCTTT AGGAAGCAGC CCCTACCCTG GGATGCCAGT CGATTCAAAG 
TTCTACAAGA TGATCAAGGA AGGGTTCCGG ATGCTCAGCC CTGAGCATGC ACCTGCTGAA 
ATGTATGACA TCATGAAGAC GTGCTGGGAT GCTGATCCCC TGAAAAGGCC GACGTTCAAG 
CAGATCGTGC AGCTAATTGA GAAGCAGATT TCAGATAGCA CCAATCATAT TTATTCCAAC 
CTCGCGAACT GCAGCCCCAA CCCAGAGCGC CCCGTGGTGG ACCATTCCGT GCGGATCAAT 
TCCGTGGGCA GCAGCGCGTC TTCCACCCAG CCTCTGCTGG TACACGAAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005627970.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628027.1 Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe16041
Clone ID Related Accession (Same CDS sequence)	XM_005627970.3 , XM_005627970.2
Accession Version	XM_005627970.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mast/stem cell growth factor receptor Kit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGAACAAA TCCATCCCCA CACCCTGTTC 
ACACCTTTGC TGATTGGCTT TGTGATCGCA GCTGGAATGA TGTGCATTAT CGTGATGATT 
CTTACCTACA AGTATCTACA GAAACCCATG TATGAAGTAC AGTGGAAGGT TGTTGAGGAG 
ATCAATGGAA ACAATTATGT TTACATAGAC CCAACACAGC TTCCTTACGA TCACAAATGG 
GAGTTTCCCA GAAACAGGCT GAGCTTTGGG AAAACTTTGG GTGCTGGTGC CTTCGGGAAA 
GTGGTTGAAG CCACTGCATA TGGCCTGATT AAGTCGGATG CGGCCATGAC TGTTGCCGTT 
AAGATGCTCA AACCAAGTGC CCATTTAACC GAACGAGAAG CCCTAATGTC TGAGCTCAAA 
GTCTTGAGTT ACCTCGGTAA TCATATGAAT ATTGTGAATC TTCTTGGAGC GTGCACCGTT 
GGAGGGCCCA CCTTGGTCAT TACTGAATAT TGTTGCTATG GTGATCTTTT GAATTTTTTG 
CGAAGGAAAC GTGATTCATT TATTTGCTCA AAGCAGGAAG ATCACGGAGA AGTGGCACTT 
TATAAGAACC TTCTGCATTC AAAGGAGTCT TCCTGCAGTG ACAGTACTAA TGAATACATG 
GACATGAAAC CCGGCGTTTC TTACGTTGTG CCAACCAAGG CAGACAAAAG GAGATCTGCG 
AGAATAGGCT CATACATAGA AAGGGATGTG ACTCCTGCCA TCATGGAAGA TGATGAGTTG 
GCTCTAGATC TAGAGGACTT GCTGAGCTTT TCTTACCAGG TGGCCAAGGG TATGGCATTC 
CTGGCCTCGA AGAATTGTAT TCACAGAGAC TTGGCTGCTA GAAATATCCT CCTTACTCAT 
GGTCGAATCA CAAAGATTTG TGATTTTGGT CTAGCCAGAG ACATCAAGAA TGATTCTAAT 
TATGTGGTCA AAGGAAACGC TCGGCTACCT GTGAAGTGGA TGGCCCCTGA GAGCATTTTC 
AACTGTGTGT ACACATTTGA AAGTGATGTC TGGTCCTATG GGATTTTTCT GTGGGAGCTC 
TTCTCTTTAG GAAGCAGCCC CTACCCTGGG ATGCCAGTCG ATTCAAAGTT CTACAAGATG 
ATCAAGGAAG GGTTCCGGAT GCTCAGCCCT GAGCATGCAC CTGCTGAAAT GTATGACATC 
ATGAAGACGT GCTGGGATGC TGATCCCCTG AAAAGGCCGA CGTTCAAGCA GATCGTGCAG 
CTAATTGAGA AGCAGATTTC AGATAGCACC AATCATATTT ATTCCAACCT CGCGAACTGC 
AGCCCCAACC CAGAGCGCCC CGTGGTGGAC CATTCCGTGC GGATCAATTC CGTGGGCAGC 
AGCGCGTCTT CCACCCAGCC TCTGCTGGTA CACGAAGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628027.1
CDS	91..3015
Translation	MRGARGAWDFLCVLLLLLLLGVQTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGENVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVDWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005627970.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627970.3

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGAACAAA TCCATCCCCA CACCCTGTTC 
ACACCTTTGC TGATTGGCTT TGTGATCGCA GCTGGAATGA TGTGCATTAT CGTGATGATT 
CTTACCTACA AGTATCTACA GAAACCCATG TATGAAGTAC AGTGGAAGGT TGTTGAGGAG 
ATCAATGGAA ACAATTATGT TTACATAGAC CCAACACAGC TTCCTTACGA TCACAAATGG 
GAGTTTCCCA GAAACAGGCT GAGCTTTGGG AAAACTTTGG GTGCTGGTGC CTTCGGGAAA 
GTGGTTGAAG CCACTGCATA TGGCCTGATT AAGTCGGATG CGGCCATGAC TGTTGCCGTT 
AAGATGCTCA AACCAAGTGC CCATTTAACC GAACGAGAAG CCCTAATGTC TGAGCTCAAA 
GTCTTGAGTT ACCTCGGTAA TCATATGAAT ATTGTGAATC TTCTTGGAGC GTGCACCGTT 
GGAGGGCCCA CCTTGGTCAT TACTGAATAT TGTTGCTATG GTGATCTTTT GAATTTTTTG 
CGAAGGAAAC GTGATTCATT TATTTGCTCA AAGCAGGAAG ATCACGGAGA AGTGGCACTT 
TATAAGAACC TTCTGCATTC AAAGGAGTCT TCCTGCAGTG ACAGTACTAA TGAATACATG 
GACATGAAAC CCGGCGTTTC TTACGTTGTG CCAACCAAGG CAGACAAAAG GAGATCTGCG 
AGAATAGGCT CATACATAGA AAGGGATGTG ACTCCTGCCA TCATGGAAGA TGATGAGTTG 
GCTCTAGATC TAGAGGACTT GCTGAGCTTT TCTTACCAGG TGGCCAAGGG TATGGCATTC 
CTGGCCTCGA AGAATTGTAT TCACAGAGAC TTGGCTGCTA GAAATATCCT CCTTACTCAT 
GGTCGAATCA CAAAGATTTG TGATTTTGGT CTAGCCAGAG ACATCAAGAA TGATTCTAAT 
TATGTGGTCA AAGGAAACGC TCGGCTACCT GTGAAGTGGA TGGCCCCTGA GAGCATTTTC 
AACTGTGTGT ACACATTTGA AAGTGATGTC TGGTCCTATG GGATTTTTCT GTGGGAGCTC 
TTCTCTTTAG GAAGCAGCCC CTACCCTGGG ATGCCAGTCG ATTCAAAGTT CTACAAGATG 
ATCAAGGAAG GGTTCCGGAT GCTCAGCCCT GAGCATGCAC CTGCTGAAAT GTATGACATC 
ATGAAGACGT GCTGGGATGC TGATCCCCTG AAAAGGCCGA CGTTCAAGCA GATCGTGCAG 
CTAATTGAGA AGCAGATTTC AGATAGCACC AATCATATTT ATTCCAACCT CGCGAACTGC 
AGCCCCAACC CAGAGCGCCC CGTGGTGGAC CATTCCGTGC GGATCAATTC CGTGGGCAGC 
AGCGCGTCTT CCACCCAGCC TCTGCTGGTA CACGAAGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005627970.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628027.1 Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X3, mRNA.

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CloneID	OCe16041
Clone ID Related Accession (Same CDS sequence)	XM_005627970.3 , XM_005627970.2
Accession Version	XM_005627970.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mast/stem cell growth factor receptor Kit isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGAACAAA TCCATCCCCA CACCCTGTTC 
ACACCTTTGC TGATTGGCTT TGTGATCGCA GCTGGAATGA TGTGCATTAT CGTGATGATT 
CTTACCTACA AGTATCTACA GAAACCCATG TATGAAGTAC AGTGGAAGGT TGTTGAGGAG 
ATCAATGGAA ACAATTATGT TTACATAGAC CCAACACAGC TTCCTTACGA TCACAAATGG 
GAGTTTCCCA GAAACAGGCT GAGCTTTGGG AAAACTTTGG GTGCTGGTGC CTTCGGGAAA 
GTGGTTGAAG CCACTGCATA TGGCCTGATT AAGTCGGATG CGGCCATGAC TGTTGCCGTT 
AAGATGCTCA AACCAAGTGC CCATTTAACC GAACGAGAAG CCCTAATGTC TGAGCTCAAA 
GTCTTGAGTT ACCTCGGTAA TCATATGAAT ATTGTGAATC TTCTTGGAGC GTGCACCGTT 
GGAGGGCCCA CCTTGGTCAT TACTGAATAT TGTTGCTATG GTGATCTTTT GAATTTTTTG 
CGAAGGAAAC GTGATTCATT TATTTGCTCA AAGCAGGAAG ATCACGGAGA AGTGGCACTT 
TATAAGAACC TTCTGCATTC AAAGGAGTCT TCCTGCAGTG ACAGTACTAA TGAATACATG 
GACATGAAAC CCGGCGTTTC TTACGTTGTG CCAACCAAGG CAGACAAAAG GAGATCTGCG 
AGAATAGGCT CATACATAGA AAGGGATGTG ACTCCTGCCA TCATGGAAGA TGATGAGTTG 
GCTCTAGATC TAGAGGACTT GCTGAGCTTT TCTTACCAGG TGGCCAAGGG TATGGCATTC 
CTGGCCTCGA AGAATTGTAT TCACAGAGAC TTGGCTGCTA GAAATATCCT CCTTACTCAT 
GGTCGAATCA CAAAGATTTG TGATTTTGGT CTAGCCAGAG ACATCAAGAA TGATTCTAAT 
TATGTGGTCA AAGGAAACGC TCGGCTACCT GTGAAGTGGA TGGCCCCTGA GAGCATTTTC 
AACTGTGTGT ACACATTTGA AAGTGATGTC TGGTCCTATG GGATTTTTCT GTGGGAGCTC 
TTCTCTTTAG GAAGCAGCCC CTACCCTGGG ATGCCAGTCG ATTCAAAGTT CTACAAGATG 
ATCAAGGAAG GGTTCCGGAT GCTCAGCCCT GAGCATGCAC CTGCTGAAAT GTATGACATC 
ATGAAGACGT GCTGGGATGC TGATCCCCTG AAAAGGCCGA CGTTCAAGCA GATCGTGCAG 
CTAATTGAGA AGCAGATTTC AGATAGCACC AATCATATTT ATTCCAACCT CGCGAACTGC 
AGCCCCAACC CAGAGCGCCC CGTGGTGGAC CATTCCGTGC GGATCAATTC CGTGGGCAGC 
AGCGCGTCTT CCACCCAGCC TCTGCTGGTA CACGAAGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628027.1
CDS	125..3049
Translation	MRGARGAWDFLCVLLLLLLLGVQTGSSQPSVSPGEPSLPSIHPAKSELIVSVGDELRLSCTDPGFVKWTFETLGQLNENTHNEWITEKAEAGHTGNYTCTNRDGLSRSIYVFVRDPAKLFLVDLPLYGKEGNDTLVRCPLTDPEVTNYSLRGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSADQKGRTVLSKKFTLKVRAAIRAVPVVSVSKTSSLLKEGEAFSVMCFIKDVSSFVDSMWIKENSQTNAQTQSNSWHHGDFNFERQEKLIISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMSTTIFVNDGENVDLIVEYEAYPKPEHQQWIYMNRTFTDKWEDYPKSDNESNIRYVSELHLTRLKGNEGGTYTFQVSNSDVNSSVTFNVYVNTKPEILTHESLTNGMLQCVVAGFPEPAVDWYFCPGAEQRCSVPIGPMDVQMQNSSLSPSGKLVVQSSIDYSAFKHNGTVECRAYNNVGRSSAFFNFAFKEQIHPHTLFTPLLIGFVIAAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISDSTNHIYSNLANCSPNPERPVVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005627970.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627970.2

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTGCT GCTGCTGCTC 
GGCGTCCAGA CAGGCTCTTC TCAACCATCT GTGAGTCCAG GGGAACCGTC TCTCCCATCC 
ATCCATCCAG CAAAATCAGA GTTAATAGTC AGTGTCGGCG ACGAGCTTAG GCTGTCCTGC 
ACCGACCCAG GATTTGTCAA GTGGACTTTT GAGACCCTGG GTCAACTGAA TGAGAACACA 
CACAACGAAT GGATCACAGA GAAGGCAGAG GCTGGCCACA CGGGCAATTA CACGTGCACC 
AACAGAGATG GCTTGAGCAG GTCCATTTAT GTGTTTGTCA GAGATCCTGC AAAGCTTTTC 
CTCGTTGACC TTCCCTTGTA TGGGAAAGAA GGCAATGATA CGCTGGTCCG CTGCCCTCTG 
ACGGACCCAG AAGTGACCAA TTACTCCCTC AGGGGGTGCG AGGGGAAGCC TCTTCCCAAG 
GACTTGACGT TCGTCGCTGA TCCCAAAGCT GGCATCACGA TCAGAAACGT GAAGCGCGAG 
TATCATCGGC TCTGCTTGCA CTGCTCTGCG GACCAGAAGG GCAGGACGGT GCTGTCCAAG 
AAATTCACCC TGAAAGTGAG GGCAGCCATC AGAGCTGTAC CAGTTGTGTC AGTATCCAAA 
ACAAGCTCTC TCCTGAAGGA AGGGGAAGCC TTCTCTGTGA TGTGCTTTAT AAAAGATGTG 
TCTAGTTTCG TGGACTCGAT GTGGATAAAG GAAAACAGCC AGACTAATGC ACAGACACAG 
AGTAATAGCT GGCATCATGG TGACTTCAAT TTTGAACGTC AGGAAAAGTT GATTATCAGC 
TCAGCAAGAG TTAATGATTC TGGAGTGTTC ATGTGTTACG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GGAAGTAGTA GATAAAGGAT TCATTAATAT CTTCCCCATG 
ATGAGTACTA CAATATTTGT AAATGATGGA GAGAATGTGG ATCTGATTGT TGAATATGAG 
GCATATCCCA AACCGGAGCA CCAGCAGTGG ATCTATATGA ACAGAACCTT CACTGATAAA 
TGGGAAGATT ATCCCAAGTC TGACAATGAA AGTAATATCA GATATGTGAG TGAACTTCAT 
CTAACCAGAT TAAAAGGGAA CGAAGGAGGC ACTTACACAT TTCAAGTGTC CAATTCCGAT 
GTCAATTCTT CGGTGACATT TAATGTTTAT GTGAACACAA AACCAGAAAT CCTGACTCAT 
GAAAGTCTCA CAAATGGCAT GCTCCAGTGT GTGGTTGCAG GATTCCCAGA GCCCGCAGTA 
GATTGGTATT TCTGTCCAGG AGCTGAGCAG AGATGTTCTG TCCCTATTGG GCCAATGGAT 
GTGCAGATGC AAAACTCGTC TCTGTCACCG TCTGGAAAAC TAGTGGTTCA GAGTTCCATC 
GATTATAGTG CCTTCAAGCA CAATGGCACA GTCGAGTGTA GGGCTTACAA CAATGTAGGC 
AGGAGTTCTG CCTTTTTTAA CTTTGCATTT AAAGAACAAA TCCATCCCCA CACCCTGTTC 
ACACCTTTGC TGATTGGCTT TGTGATCGCA GCTGGAATGA TGTGCATTAT CGTGATGATT 
CTTACCTACA AGTATCTACA GAAACCCATG TATGAAGTAC AGTGGAAGGT TGTTGAGGAG 
ATCAATGGAA ACAATTATGT TTACATAGAC CCAACACAGC TTCCTTACGA TCACAAATGG 
GAGTTTCCCA GAAACAGGCT GAGCTTTGGG AAAACTTTGG GTGCTGGTGC CTTCGGGAAA 
GTGGTTGAAG CCACTGCATA TGGCCTGATT AAGTCGGATG CGGCCATGAC TGTTGCCGTT 
AAGATGCTCA AACCAAGTGC CCATTTAACC GAACGAGAAG CCCTAATGTC TGAGCTCAAA 
GTCTTGAGTT ACCTCGGTAA TCATATGAAT ATTGTGAATC TTCTTGGAGC GTGCACCGTT 
GGAGGGCCCA CCTTGGTCAT TACTGAATAT TGTTGCTATG GTGATCTTTT GAATTTTTTG 
CGAAGGAAAC GTGATTCATT TATTTGCTCA AAGCAGGAAG ATCACGGAGA AGTGGCACTT 
TATAAGAACC TTCTGCATTC AAAGGAGTCT TCCTGCAGTG ACAGTACTAA TGAATACATG 
GACATGAAAC CCGGCGTTTC TTACGTTGTG CCAACCAAGG CAGACAAAAG GAGATCTGCG 
AGAATAGGCT CATACATAGA AAGGGATGTG ACTCCTGCCA TCATGGAAGA TGATGAGTTG 
GCTCTAGATC TAGAGGACTT GCTGAGCTTT TCTTACCAGG TGGCCAAGGG TATGGCATTC 
CTGGCCTCGA AGAATTGTAT TCACAGAGAC TTGGCTGCTA GAAATATCCT CCTTACTCAT 
GGTCGAATCA CAAAGATTTG TGATTTTGGT CTAGCCAGAG ACATCAAGAA TGATTCTAAT 
TATGTGGTCA AAGGAAACGC TCGGCTACCT GTGAAGTGGA TGGCCCCTGA GAGCATTTTC 
AACTGTGTGT ACACATTTGA AAGTGATGTC TGGTCCTATG GGATTTTTCT GTGGGAGCTC 
TTCTCTTTAG GAAGCAGCCC CTACCCTGGG ATGCCAGTCG ATTCAAAGTT CTACAAGATG 
ATCAAGGAAG GGTTCCGGAT GCTCAGCCCT GAGCATGCAC CTGCTGAAAT GTATGACATC 
ATGAAGACGT GCTGGGATGC TGATCCCCTG AAAAGGCCGA CGTTCAAGCA GATCGTGCAG 
CTAATTGAGA AGCAGATTTC AGATAGCACC AATCATATTT ATTCCAACCT CGCGAACTGC 
AGCCCCAACC CAGAGCGCCC CGTGGTGGAC CATTCCGTGC GGATCAATTC CGTGGGCAGC 
AGCGCGTCTT CCACCCAGCC TCTGCTGGTA CACGAAGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A decrease in ubiquitination and resulting prolonged life-span of KIT underlies the KIT overexpression-mediated imatinib resistance of KIT mutation-driven canine mast cell tumor cells.
Oncology reports38(4)2543-2550(2017 Oct)
Kobayashi M,Kuroki S,Kurita S,Miyamoto R,Tani H,Tamura K,Bonkobara M

Genomic profiling of canine mast cell tumors identifies DNA copy number aberrations associated with KIT mutations and high histological grade.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology25(2)129-143(2017 Jun)
Mochizuki H,Thomas R,Moroff S,Breen M

KTN0158, a Humanized Anti-KIT Monoclonal Antibody, Demonstrates Biologic Activity against both Normal and Malignant Canine Mast Cells.
Clinical cancer research : an official journal of the American Association for Cancer Research23(10)2565-2574(2017 May)
London CA,Gardner HL,Rippy S,Post G,La Perle K,Crew L,Lopresti-Morrow L,Garton AJ,McMahon G,LaVallee TM,Gedrich R

Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas.
BMC veterinary research12(1)142(2016 Jul)
Chen YC,Liao JW,Hsu WL,Chang SC

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KIT cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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