RPE65 cDNA ORF clone, Canis lupus familiaris(dog)

RPE65 gene therapy slows cone loss in Rpe65-deficient dogs.
Mowat FM, Breuwer AR, Bartoe JT, Annear MJ, Zhang Z, Smith AJ, Bainbridge JW, Petersen-Jones SM, Ali RR
Gene therapy20(5)545-55(2013 May)

Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis.
Hern?ndez M, Pearce-Kelling SE, Rodriguez FD, Aguirre GD, Vecino E
Investigative ophthalmology & visual science51(12)6793-802(2010 Dec)

Improvement of visual performance with intravitreal administration of 9-cis-retinal in Rpe65-mutant dogs.
Gearhart PM, Gearhart C, Thompson DA, Petersen-Jones SM
Archives of ophthalmology (Chicago, Ill. : 1960)128(11)1442-8(2010 Nov)

Morphological aspects related to long-term functional improvement of the retina in the 4 years following rAAV-mediated gene transfer in the RPE65 null mutation dog.
Narfstr?m K, Seeliger M, Lai CM, Vaegan, Katz M, Rakoczy EP, Rem? C
Advances in experimental medicine and biology613139-46(2008)

Effect of Leu/Met variation at residue 450 on isomerase activity and protein expression of RPE65 and its modulation by variation at other residues.
Redmond TM, Weber CH, Poliakov E, Yu S, Gentleman S
Molecular vision131813-21(2007 Sep)

We examined the retinas of Rpe65-deficient dogs after RPE65 gene therapy to evaluate the preservation of rods and cone photoreceptor subtypes. We found that gene therapy preserves both rods and cones
Title: RPE65 gene therapy slows cone loss in Rpe65-deficient dogs.

The structure of the retina is well preserved in the mutant retina, but several molecular changes take place in photoreceptors and retinal neurons, whereas others reflect a change in localization of the examined proteins.
Title: Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis.

Our findings establish that 9-cis-retinoid therapy can restore visual function in a canine model of human disease resulting from RPE65 mutations.
Title: Improvement of visual performance with intravitreal administration of 9-cis-retinal in Rpe65-mutant dogs.

Morphological aspects related to long-term functional improvement of the retina in the 4 years following rAAV-mediated gene transfer in the RPE65 null mutation.
Title: Morphological aspects related to long-term functional improvement of the retina in the 4 years following rAAV-mediated gene transfer in the RPE65 null mutation dog.

Wildtype dog RPE65 is more active than wildtype mouse RPE65. The effect of Met at aa450 is more severe in mouse RPE65 than in dog. Effects of variation at residues 446 (K or R) modulate variation at aa450.
Title: Effect of Leu/Met variation at residue 450 on isomerase activity and protein expression of RPE65 and its modulation by variation at other residues.