IL4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL4
Entrez Gene ID	403785
Full Name	interleukin 4
Synonyms	IL-4
General protein information	Preferred Names interleukin 4 Names interleukin-4 B-cell stimulatory factor 1 BSF-1 lymphocyte stimulatory factor 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003159.1	NP_001003159.1	interleukin-4 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Il4	NP_958427.1
Homo sapiens (human)	IL4	NP_000580.1
Pan troglodytes (chimpanzee)	IL4	NP_001011714.1
	IL4	NP_001028076.1
Bos taurus (cattle)	IL4	NP_776346.1
Mus musculus (house mouse)	Il4	NP_067258.1
Canis lupus familiaris (dog)	IL4	NP_001003159.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of expression of inflammatory cytokines in the spinal cord between young adult and aged beagle dogs.
Lee DH, Ahn JH, Park JH, Yan BC, Cho JH, Kim IH, Lee JC, Jang SH, Lee MH, Hwang IK, Moon SM, Lee B, Cho JH, Shin HC, Kim JS, Won MH
Cellular and molecular neurobiology33(5)615-24(2013 Jul)

Cytokine gene expression in the tissues of dogs infected by Leishmania infantum.
Barbosa MA, Alexandre-Pires G, Soares-Clemente M, Marques C, Rodrigues OR, De Brito TV, Da Fonseca IP, Alves LC, Santos-Gomes GM
Journal of comparative pathology145(4)336-44(2011 Nov)

Evidence for regulated interleukin-4 expression in chondrocyte-scaffolds under in vitro inflammatory conditions.
Rai MF, Graeve T, Twardziok S, Schmidt MF
PloS one6(10)e25749(2011)

Co-expression of insulin-like growth factor-1 and interleukin-4 in an in vitro inflammatory model.
Manning K, Rachakonda PS, Rai MF, Schmidt MF
Cytokine50(3)297-305(2010 Jun)

Effects of polyunsaturated fatty acids on isolated canine peripheral blood mononuclear cells and cytokine expression (IL-4, IFN-gamma, TGF-beta) in healthy and atopic dogs.
Stehle ME, Hanczaruk M, Schwarz SC, G?bel TW, Mueller RS
Veterinary dermatology21(1)112-7(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared.
Title: Comparison of expression of inflammatory cytokines in the spinal cord between young adult and aged beagle dogs.

In dogs with leishmaniasis, there was high expression of genes encoding IL-4 in blood leucocytes. The predominance of IL-4 gene expression in the blood of asymptomatic dogs may favour parasite replication.
Title: Cytokine gene expression in the tissues of dogs infected by Leishmania infantum.

The anti-inflammatory and anabolic effects of regulated expression of IL-4 in chondrocyte-scaffolds under in vitro inflammatory conditions, were investigated.
Title: Evidence for regulated interleukin-4 expression in chondrocyte-scaffolds under in vitro inflammatory conditions.

This study focuses on the co-expression of interleukin-4 (IL-4) and insulin-like-growth factor-1 (IGF-1), which specifically target inflammation and cartilage repair, respectively.
Title: Co-expression of insulin-like growth factor-1 and interleukin-4 in an in vitro inflammatory model.

These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription.
Title: Effects of polyunsaturated fatty acids on isolated canine peripheral blood mononuclear cells and cytokine expression (IL-4, IFN-gamma, TGF-beta) in healthy and atopic dogs.

The following IL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34308	NM_001003159.1 Latest version!	Canis lupus familiaris interleukin 4 (IL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL4 ( NM_001003159.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003159.1 Canis lupus familiaris interleukin 4 (IL4), mRNA.

Price & Availability↑

CloneID	OCe34308
Clone ID Related Accession (Same CDS sequence)	NM_001003159.1
Accession Version	NM_001003159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	interleukin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF239917.1. ##Evidence-Data-START## Transcript exon combination :: AF239917.1, AF083270.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN07419803, SAMN07419815 [ECO:0000350] ##Evidence-Data-END##

ATGGGTCTCA CCTCCCAACT GATTCCAACT CTGGTCTGCT TACTAGCACT CACCAGCACC 
TTTGTCCACG GACATAACTT CAATATTACT ATTAAAGAGA TCATCAAAAT GTTGAACATC 
CTCACAGCGA GAAACGACTC GTGCATGGAG CTGACTGTCA AGGACGTCTT CACTGCTCCA 
AAGAACACAA GCGATAAGGA AATCTTCTGC AGAGCTGCTA CTGTACTGCG GCAGATCTAT 
ACACACAACT GCTCCAACAG ATATCTCAGA GGACTCTACA GGAACCTCAG CAGCATGGCA 
AACAAGACCT GTTCTATGAA TGAAATCAAG AAGAGTACAC TGAAAGACTT CTTGGAAAGG 
CTAAAAGTGA TCATGCAGAA GAAATACTAC AGGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003159.1
CDS	64..462
Translation	MGLTSQLIPTLVCLLALTSTFVHGHNFNITIKEIIKMLNILTARNDSCMELTVKDVFTAPKNTSDKEIFCRAATVLRQIYTHNCSNRYLRGLYRNLSSMANKTCSMNEIKKSTLKDFLERLKVIMQKKYYRH

Target ORF information:

RefSeq Version	NM_001003159.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris interleukin 4 (IL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003159.1

ORF Insert Sequence:

ATGGGTCTCA CCTCCCAACT GATTCCAACT CTGGTCTGCT TACTAGCACT CACCAGCACC 
TTTGTCCACG GACATAACTT CAATATTACT ATTAAAGAGA TCATCAAAAT GTTGAACATC 
CTCACAGCGA GAAACGACTC GTGCATGGAG CTGACTGTCA AGGACGTCTT CACTGCTCCA 
AAGAACACAA GCGATAAGGA AATCTTCTGC AGAGCTGCTA CTGTACTGCG GCAGATCTAT 
ACACACAACT GCTCCAACAG ATATCTCAGA GGACTCTACA GGAACCTCAG CAGCATGGCA 
AACAAGACCT GTTCTATGAA TGAAATCAAG AAGAGTACAC TGAAAGACTT CTTGGAAAGG 
CTAAAAGTGA TCATGCAGAA GAAATACTAC AGGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of expression of inflammatory cytokines in the spinal cord between young adult and aged beagle dogs.
Cellular and molecular neurobiology33(5)615-24(2013 Jul)
Lee DH,Ahn JH,Park JH,Yan BC,Cho JH,Kim IH,Lee JC,Jang SH,Lee MH,Hwang IK,Moon SM,Lee B,Cho JH,Shin HC,Kim JS,Won MH

Cytokine gene expression in the tissues of dogs infected by Leishmania infantum.
Journal of comparative pathology145(4)336-44(2011 Nov)
Barbosa MA,Alexandre-Pires G,Soares-Clemente M,Marques C,Rodrigues OR,De Brito TV,Da Fonseca IP,Alves LC,Santos-Gomes GM

Evidence for regulated interleukin-4 expression in chondrocyte-scaffolds under in vitro inflammatory conditions.
PloS one6(10)e25749(2011)
Rai MF,Graeve T,Twardziok S,Schmidt MF

Co-expression of insulin-like growth factor-1 and interleukin-4 in an in vitro inflammatory model.
Cytokine50(3)297-305(2010 Jun)
Manning K,Rachakonda PS,Rai MF,Schmidt MF

Effects of polyunsaturated fatty acids on isolated canine peripheral blood mononuclear cells and cytokine expression (IL-4, IFN-gamma, TGF-beta) in healthy and atopic dogs.
Veterinary dermatology21(1)112-7(2010 Feb)
Stehle ME,Hanczaruk M,Schwarz SC,G?bel TW,Mueller RS

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IL4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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