NOS3 cDNA ORF clone, Canis lupus familiaris(Dog)

The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe293770 XM_022403591.1
Latest version!
Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe34317 NM_001003158.2
Latest version!
Canis lupus familiaris nitric oxide synthase 3 (NOS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17873 XM_005629459.3
Latest version!
Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17874 XM_005629460.3
Latest version!
Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17875 XM_005629461.3
Latest version!
Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17876 XM_005629462.2
Latest version!
Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe17873 XM_005629459.2 Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17874 XM_005629460.2 Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17875 XM_005629461.2 Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe293770
    Clone ID Related Accession (Same CDS sequence) XM_022403591.1
    Accession Version XM_022403591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, endothelial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGAGAT GTCCCAGCAA GGAGATAAGG GACAGACCAG ATGTGTGTGG TGCAGGGCAG 
    GAAGCAGTCA GTGTGGGTGC TGATGATCAG GTGTTTGATG CCCGGGATTG CAGCTCTGCC
    CAGGAGATGT TCACCTACAT CTGCAACCAC ATCAAGTATG CCACCAACCG GGGCAACCTC
    CGCTCGGCCA TCACAGTGTT CCCCCAGCGT GCCTCAGGCC GCGGAGACTT TCGAATTTGG
    AACAGCCAGC TGGTGCGCTA TGCGGGCTAC AGGCAGCAGG ATGGCTCTGT GCGGGGGGAC
    CCAGCCAACG TGGAGATCAC TGAGCTCTGC ATCCAGCATG GCTGGACTCC AGGAAACGGC
    CGCTTTGATG TGCTGCCCCT ACTGCTCCAG GCCCCAGATG AACCCCCAGA ACTCTTTGCT
    CTGCCCCCCG AGCTGGTTCT CGAGGTGCCC CTGGAGCACC CCACGCTGGA GTGGTTTGCG
    GCCCTGGGCC TGCGCTGGTA TGCCCTCCCA GCGGTGAGCA ACATGCTGCT GGAAATTGGG
    GGGCTGGAGT TCCCTGCGGC CCCCTTCAGC GGCTGGTACA TGAGCACTGA GATTGGCACG
    CGGAACCTGT GTGACCCTCA TCGATACAAC ATCCTGGAGG ATGTGGCTGT CTGCATGGAC
    TTGGATACCA GGACCACCTC ATCCCTGTGG AAAGACAAGG CAGCAGTGGA AATCAACTTG
    GCTGTGCTGC ACAGTTACCA GCTGGCCAAA GTGACCATCG TGGACCACCA TGCTGCCACA
    GCCTCCTTCA TGAAGCACCT GGAGAACGAG CAGAAGGCCA GGGGGGGTTG CCCTGCTGAC
    TGGGCCTGGA TCGTGCCCCC CATCTCTGGC AGCCTCACCC CTGTTTTCCA TCAGGAGATG
    GTCAACTATG TCCTGTCCCC GGCCTTCCGC TATCAGACAG ACCCGTGGAA GGGGAGTGCA
    TCCAAGGGTG CCGGCGTCAC CAGGAAGAAA ACCTTTAAGG AAGTGGCCAA TGCGGTGAAG
    ATCTCTGCCT CACTCATGGG CACTGTGATG GCAAAGCGAG TGAAGGCGAC AATCCTCTAT
    GGCTCCGAGA CTGGCCGGGC CCAGAGCTAT GCACAGCAGC TGGGGAGACT CTTCCGGAAG
    GCTTTCGACC CCCGGGTCCT GTGCATGGAT GAGTATGACG TGGTGTCCCT TGAGCATGAG
    ACGCTGGTGT TGGTGGTGAC CAGCACATTT GGGAATGGTG ATCCCCCAGA GAATGGAGAG
    AGTTTTGCGG CAGCCCTGAT GGAGATGTCG GGTCCCTACA ACAGCTCCCC TCGGCCAGAA
    CAGCACAAGA GTTACAAAAT CCGCTTCAAC AGTGTCTCCT GCTCAGACCC ACTGGTGTCC
    TCCTGGAGGC GGAAGAGAAA GGAGTCCAGT AACACAGACA GTGCTGGGGC ACTGGGTACC
    CTCAGGTTCT GTGTGTTCGG GCTGGGCTCC CGGGCATACC CCCACTTTTG TGCCTTTGCT
    CGTGCGGTGG ACACACGGCT GGAAGAGCTG GGTGGGGAGC GGCTGCTGCA GCTGGGCCAA
    GGAGATGAGC TGTGTGGCCA GGAGGAGGCC TTCCGAGGCT GGGCCCAGGC CGCCTTCCAG
    GCCTCCTGTG AGACCTTCTG CGTGGGAGAG GATGCCAAGG CTGCCGCCCG GGACATATTC
    AGCCCCAAAC GGACCTGGAA ACGCCAGAGG TACCGGCTGA GCGCCCAGGC TGAGGGCCTC
    CAGCTGCTGC CAGGACTGAT CCATGTGCAC AGGAGGAAGA TGGTCCAGGC TACTGTCCTC
    GCAGTGGAAA ACCTGCAAAG CAGCAAGTCC ACCCGGGCCA CAATCCTGGT GCGCCTGGAC
    ACTGGAAGCC AGGAGGCGCT GCAGTACCAG CCAGGGGACC ACATTGGCAT CTGCCCGCCC
    AACCGGCCTG GCCTTGTGGA GGCGCTGCTG AGCCGGGTGG AGGATCCACC GCCACCAGGA
    GAGCCAGTGG CAGTGGAGCA GCTGGAGAAG GGCAGCCCCG ATCTCCCCTG TACACCATCA
    CCAGGTGGCC CACCCCCCAG CTGGGTTCGG GACCCTCGCC TGCCCCCATG CACACTGCGC
    CAGGCTCTCA CCTTCTTCCT GGACATCACT TCCCCGCCCA GCCCTCAACT CCTTCGACTG
    CTCAGCACCC TGGCTGAAGA GTCCAGTGAA CAGCAGGAGC TTGAGAGCCT CAGCCAGGAC
    CCCCGGCGCT ATGAGGAATG GAAGTGGTTC CGCTGCCCCA CACTGCTGGA GGTACTGGAA
    CAGTTCCCAT CGGTGGCACT GCCCGCCCCC CTGCTCCTCA CCCAGCTGCC CCTGCTCCAG
    CCCCGGTACT ACTCGGTCAG CTCAGCACCC AGTGCCCACC CAGGAGAGAT CCACCTCACA
    GTAGCTGTGC TGGCATACAG GACACAGGAT GGACTGGGCC CCCTTCATTA TGGAGTCTGC
    TCCACATGGC TAAGCCAACT TAAGGCCGGA GATCCTGTAC CCTGCTTCAT CAGGGGGGCT
    CCCTCCTTCC GGCTGCCACC TGATCCCAGT CTGCCTTGCA TCTTAGTGGG TCCTGGTACG
    GGTATTGCCC CCTTTCGGGG TTTCTGGCAG GAGCGGCTGC ACGACATTGA CAGCAAAGGG
    CTGCAGCCCG CCCCCATGAC TCTGGTGTTC GGCTGCCGAT GCTCCCAGCT CGACCACCTT
    TACCGCGACG AGGTGCAGGA TGCCCAGCAG CGCGGAGTTT TTGGGCGCGT CCTCACCGCC
    TTCTCGCGGG AGCCCGACAG CCCTAAGACG TACGTGCAGG ACATCCTGCG GACGGAGCTG
    GCTGCTGAGG TGCACCGCGT GCTGTGCCTC GAGCGGGGCC ACATGTTTGT GTGCGGCGAC
    GTCACCATGG CAACCAGCGT CCTGCAGACG GTGCAGCGAA TCCTGGCGAC GGAGGGCGAC
    ATGGAGCTGG ACGAGGCCGG CGACGTCATC GGCGTGCTGC GGGATCAGCA ACGCTATCAC
    GAGGACATTT TCGGGCTCAC ACTGCGCACC CAGGAGGTGA CAAGCCGCAT ACGCACCCAG
    AGCTTCTCCT TGCAGGAGCG GCATCTGCGG GGCGCGGTGC CCTGGGCGTT GGACCCGCCC
    GGCCCAGACA CCGCCGGCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259299.1
    CDS749..3892
    Translation

    Target ORF information:

    RefSeq Version XM_022403591.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGAGAT GTCCCAGCAA GGAGATAAGG GACAGACCAG ATGTGTGTGG TGCAGGGCAG 
    GAAGCAGTCA GTGTGGGTGC TGATGATCAG GTGTTTGATG CCCGGGATTG CAGCTCTGCC
    CAGGAGATGT TCACCTACAT CTGCAACCAC ATCAAGTATG CCACCAACCG GGGCAACCTC
    CGCTCGGCCA TCACAGTGTT CCCCCAGCGT GCCTCAGGCC GCGGAGACTT TCGAATTTGG
    AACAGCCAGC TGGTGCGCTA TGCGGGCTAC AGGCAGCAGG ATGGCTCTGT GCGGGGGGAC
    CCAGCCAACG TGGAGATCAC TGAGCTCTGC ATCCAGCATG GCTGGACTCC AGGAAACGGC
    CGCTTTGATG TGCTGCCCCT ACTGCTCCAG GCCCCAGATG AACCCCCAGA ACTCTTTGCT
    CTGCCCCCCG AGCTGGTTCT CGAGGTGCCC CTGGAGCACC CCACGCTGGA GTGGTTTGCG
    GCCCTGGGCC TGCGCTGGTA TGCCCTCCCA GCGGTGAGCA ACATGCTGCT GGAAATTGGG
    GGGCTGGAGT TCCCTGCGGC CCCCTTCAGC GGCTGGTACA TGAGCACTGA GATTGGCACG
    CGGAACCTGT GTGACCCTCA TCGATACAAC ATCCTGGAGG ATGTGGCTGT CTGCATGGAC
    TTGGATACCA GGACCACCTC ATCCCTGTGG AAAGACAAGG CAGCAGTGGA AATCAACTTG
    GCTGTGCTGC ACAGTTACCA GCTGGCCAAA GTGACCATCG TGGACCACCA TGCTGCCACA
    GCCTCCTTCA TGAAGCACCT GGAGAACGAG CAGAAGGCCA GGGGGGGTTG CCCTGCTGAC
    TGGGCCTGGA TCGTGCCCCC CATCTCTGGC AGCCTCACCC CTGTTTTCCA TCAGGAGATG
    GTCAACTATG TCCTGTCCCC GGCCTTCCGC TATCAGACAG ACCCGTGGAA GGGGAGTGCA
    TCCAAGGGTG CCGGCGTCAC CAGGAAGAAA ACCTTTAAGG AAGTGGCCAA TGCGGTGAAG
    ATCTCTGCCT CACTCATGGG CACTGTGATG GCAAAGCGAG TGAAGGCGAC AATCCTCTAT
    GGCTCCGAGA CTGGCCGGGC CCAGAGCTAT GCACAGCAGC TGGGGAGACT CTTCCGGAAG
    GCTTTCGACC CCCGGGTCCT GTGCATGGAT GAGTATGACG TGGTGTCCCT TGAGCATGAG
    ACGCTGGTGT TGGTGGTGAC CAGCACATTT GGGAATGGTG ATCCCCCAGA GAATGGAGAG
    AGTTTTGCGG CAGCCCTGAT GGAGATGTCG GGTCCCTACA ACAGCTCCCC TCGGCCAGAA
    CAGCACAAGA GTTACAAAAT CCGCTTCAAC AGTGTCTCCT GCTCAGACCC ACTGGTGTCC
    TCCTGGAGGC GGAAGAGAAA GGAGTCCAGT AACACAGACA GTGCTGGGGC ACTGGGTACC
    CTCAGGTTCT GTGTGTTCGG GCTGGGCTCC CGGGCATACC CCCACTTTTG TGCCTTTGCT
    CGTGCGGTGG ACACACGGCT GGAAGAGCTG GGTGGGGAGC GGCTGCTGCA GCTGGGCCAA
    GGAGATGAGC TGTGTGGCCA GGAGGAGGCC TTCCGAGGCT GGGCCCAGGC CGCCTTCCAG
    GCCTCCTGTG AGACCTTCTG CGTGGGAGAG GATGCCAAGG CTGCCGCCCG GGACATATTC
    AGCCCCAAAC GGACCTGGAA ACGCCAGAGG TACCGGCTGA GCGCCCAGGC TGAGGGCCTC
    CAGCTGCTGC CAGGACTGAT CCATGTGCAC AGGAGGAAGA TGGTCCAGGC TACTGTCCTC
    GCAGTGGAAA ACCTGCAAAG CAGCAAGTCC ACCCGGGCCA CAATCCTGGT GCGCCTGGAC
    ACTGGAAGCC AGGAGGCGCT GCAGTACCAG CCAGGGGACC ACATTGGCAT CTGCCCGCCC
    AACCGGCCTG GCCTTGTGGA GGCGCTGCTG AGCCGGGTGG AGGATCCACC GCCACCAGGA
    GAGCCAGTGG CAGTGGAGCA GCTGGAGAAG GGCAGCCCCG ATCTCCCCTG TACACCATCA
    CCAGGTGGCC CACCCCCCAG CTGGGTTCGG GACCCTCGCC TGCCCCCATG CACACTGCGC
    CAGGCTCTCA CCTTCTTCCT GGACATCACT TCCCCGCCCA GCCCTCAACT CCTTCGACTG
    CTCAGCACCC TGGCTGAAGA GTCCAGTGAA CAGCAGGAGC TTGAGAGCCT CAGCCAGGAC
    CCCCGGCGCT ATGAGGAATG GAAGTGGTTC CGCTGCCCCA CACTGCTGGA GGTACTGGAA
    CAGTTCCCAT CGGTGGCACT GCCCGCCCCC CTGCTCCTCA CCCAGCTGCC CCTGCTCCAG
    CCCCGGTACT ACTCGGTCAG CTCAGCACCC AGTGCCCACC CAGGAGAGAT CCACCTCACA
    GTAGCTGTGC TGGCATACAG GACACAGGAT GGACTGGGCC CCCTTCATTA TGGAGTCTGC
    TCCACATGGC TAAGCCAACT TAAGGCCGGA GATCCTGTAC CCTGCTTCAT CAGGGGGGCT
    CCCTCCTTCC GGCTGCCACC TGATCCCAGT CTGCCTTGCA TCTTAGTGGG TCCTGGTACG
    GGTATTGCCC CCTTTCGGGG TTTCTGGCAG GAGCGGCTGC ACGACATTGA CAGCAAAGGG
    CTGCAGCCCG CCCCCATGAC TCTGGTGTTC GGCTGCCGAT GCTCCCAGCT CGACCACCTT
    TACCGCGACG AGGTGCAGGA TGCCCAGCAG CGCGGAGTTT TTGGGCGCGT CCTCACCGCC
    TTCTCGCGGG AGCCCGACAG CCCTAAGACG TACGTGCAGG ACATCCTGCG GACGGAGCTG
    GCTGCTGAGG TGCACCGCGT GCTGTGCCTC GAGCGGGGCC ACATGTTTGT GTGCGGCGAC
    GTCACCATGG CAACCAGCGT CCTGCAGACG GTGCAGCGAA TCCTGGCGAC GGAGGGCGAC
    ATGGAGCTGG ACGAGGCCGG CGACGTCATC GGCGTGCTGC GGGATCAGCA ACGCTATCAC
    GAGGACATTT TCGGGCTCAC ACTGCGCACC CAGGAGGTGA CAAGCCGCAT ACGCACCCAG
    AGCTTCTCCT TGCAGGAGCG GCATCTGCGG GGCGCGGTGC CCTGGGCGTT GGACCCGCCC
    GGCCCAGACA CCGCCGGCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34317
    Clone ID Related Accession (Same CDS sequence) NM_001003158.2
    Accession Version NM_001003158.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, endothelial
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010272.1. On Oct 27, 2011 this sequence version replaced NM_001003158.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF143503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA2240903 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGGT
    GGCCCACCCC CCAGCTGGGT TCGGGACCCT CGCCTGCCCC CATGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACTTCCCCG CCCAGCCCTC AACTCCTTCG ACTGCTCAGC
    ACCCTGGCTG AAGAGTCCAG TGAACAGCAG GAGCTTGAGA GCCTCAGCCA GGACCCCCGG
    CGCTATGAGG AATGGAAGTG GTTCCGCTGC CCCACACTGC TGGAGGTACT GGAACAGTTC
    CCATCGGTGG CACTGCCCGC CCCCCTGCTC CTCACCCAGC TGCCCCTGCT CCAGCCCCGG
    TACTACTCGG TCAGCTCAGC ACCCAGTGCC CACCCAGGAG AGATCCACCT CACAGTAGCT
    GTGCTGGCAT ACAGGACACA GGATGGACTG GGCCCCCTTC ATTATGGAGT CTGCTCCACA
    TGGCTAAGCC AACTTAAGGC CGGAGATCCT GTACCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CACCTGATCC CAGTCTGCCT TGCATCTTAG TGGGTCCTGG TACGGGTATT
    GCCCCCTTTC GGGGTTTCTG GCAGGAGCGG CTGCACGACA TTGACAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACTCTGGT GTTCGGCTGC CGATGCTCCC AGCTCGACCA CCTTTACCGC
    GACGAGGTGC AGGATGCCCA GCAGCGCGGA GTTTTTGGGC GCGTCCTCAC CGCCTTCTCG
    CGGGAGCCCG ACAGCCCTAA GACGTACGTG CAGGACATCC TGCGGACGGA GCTGGCTGCT
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTGTGCGG CGACGTCACC
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGAATCCTGG CGACGGAGGG CGACATGGAG
    CTGGACGAGG CCGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGGC TCACACTGCG CACCCAGGAG GTGACAAGCC GCATACGCAC CCAGAGCTTC
    TCCTTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CGTTGGACCC GCCCGGCCCA
    GACACCGCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001003158.2
    CDS241..3858
    Translation

    Target ORF information:

    RefSeq Version NM_001003158.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (NOS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001003158.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGGT
    GGCCCACCCC CCAGCTGGGT TCGGGACCCT CGCCTGCCCC CATGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACTTCCCCG CCCAGCCCTC AACTCCTTCG ACTGCTCAGC
    ACCCTGGCTG AAGAGTCCAG TGAACAGCAG GAGCTTGAGA GCCTCAGCCA GGACCCCCGG
    CGCTATGAGG AATGGAAGTG GTTCCGCTGC CCCACACTGC TGGAGGTACT GGAACAGTTC
    CCATCGGTGG CACTGCCCGC CCCCCTGCTC CTCACCCAGC TGCCCCTGCT CCAGCCCCGG
    TACTACTCGG TCAGCTCAGC ACCCAGTGCC CACCCAGGAG AGATCCACCT CACAGTAGCT
    GTGCTGGCAT ACAGGACACA GGATGGACTG GGCCCCCTTC ATTATGGAGT CTGCTCCACA
    TGGCTAAGCC AACTTAAGGC CGGAGATCCT GTACCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CACCTGATCC CAGTCTGCCT TGCATCTTAG TGGGTCCTGG TACGGGTATT
    GCCCCCTTTC GGGGTTTCTG GCAGGAGCGG CTGCACGACA TTGACAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACTCTGGT GTTCGGCTGC CGATGCTCCC AGCTCGACCA CCTTTACCGC
    GACGAGGTGC AGGATGCCCA GCAGCGCGGA GTTTTTGGGC GCGTCCTCAC CGCCTTCTCG
    CGGGAGCCCG ACAGCCCTAA GACGTACGTG CAGGACATCC TGCGGACGGA GCTGGCTGCT
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTGTGCGG CGACGTCACC
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGAATCCTGG CGACGGAGGG CGACATGGAG
    CTGGACGAGG CCGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGGC TCACACTGCG CACCCAGGAG GTGACAAGCC GCATACGCAC CCAGAGCTTC
    TCCTTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CGTTGGACCC GCCCGGCCCA
    GACACCGCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17873
    Clone ID Related Accession (Same CDS sequence) XM_005629459.2 , XM_005629459.3
    Accession Version XM_005629459.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, endothelial isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGAT
    CTCCCCTGTA CACCATCACC AGGTGGCCCA CCCCCCAGCT GGGTTCGGGA CCCTCGCCTG
    CCCCCATGCA CACTGCGCCA GGCTCTCACC TTCTTCCTGG ACATCACTTC CCCGCCCAGC
    CCTCAACTCC TTCGACTGCT CAGCACCCTG GCTGAAGAGT CCAGTGAACA GCAGGAGCTT
    GAGAGCCTCA GCCAGGACCC CCGGCGCTAT GAGGAATGGA AGTGGTTCCG CTGCCCCACA
    CTGCTGGAGG TACTGGAACA GTTCCCATCG GTGGCACTGC CCGCCCCCCT GCTCCTCACC
    CAGCTGCCCC TGCTCCAGCC CCGGTACTAC TCGGTCAGCT CAGCACCCAG TGCCCACCCA
    GGAGAGATCC ACCTCACAGT AGCTGTGCTG GCATACAGGA CACAGGATGG ACTGGGCCCC
    CTTCATTATG GAGTCTGCTC CACATGGCTA AGCCAACTTA AGGCCGGAGA TCCTGTACCC
    TGCTTCATCA GGGGGGCTCC CTCCTTCCGG CTGCCACCTG ATCCCAGTCT GCCTTGCATC
    TTAGTGGGTC CTGGTACGGG TATTGCCCCC TTTCGGGGTT TCTGGCAGGA GCGGCTGCAC
    GACATTGACA GCAAAGGGCT GCAGCCCGCC CCCATGACTC TGGTGTTCGG CTGCCGATGC
    TCCCAGCTCG ACCACCTTTA CCGCGACGAG GTGCAGGATG CCCAGCAGCG CGGAGTTTTT
    GGGCGCGTCC TCACCGCCTT CTCGCGGGAG CCCGACAGCC CTAAGACGTA CGTGCAGGAC
    ATCCTGCGGA CGGAGCTGGC TGCTGAGGTG CACCGCGTGC TGTGCCTCGA GCGGGGCCAC
    ATGTTTGTGT GCGGCGACGT CACCATGGCA ACCAGCGTCC TGCAGACGGT GCAGCGAATC
    CTGGCGACGG AGGGCGACAT GGAGCTGGAC GAGGCCGGCG ACGTCATCGG CGTGCTGCGG
    GATCAGCAAC GCTATCACGA GGACATTTTC GGGCTCACAC TGCGCACCCA GGAGGTGACA
    AGCCGCATAC GCACCCAGAG CTTCTCCTTG CAGGAGCGGC ATCTGCGGGG CGCGGTGCCC
    TGGGCGTTGG ACCCGCCCGG CCCAGACACC GCCGGCCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629516.1
    CDS73..3714
    Translation

    Target ORF information:

    RefSeq Version XM_005629459.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGAT
    CTCCCCTGTA CACCATCACC AGGTGGCCCA CCCCCCAGCT GGGTTCGGGA CCCTCGCCTG
    CCCCCATGCA CACTGCGCCA GGCTCTCACC TTCTTCCTGG ACATCACTTC CCCGCCCAGC
    CCTCAACTCC TTCGACTGCT CAGCACCCTG GCTGAAGAGT CCAGTGAACA GCAGGAGCTT
    GAGAGCCTCA GCCAGGACCC CCGGCGCTAT GAGGAATGGA AGTGGTTCCG CTGCCCCACA
    CTGCTGGAGG TACTGGAACA GTTCCCATCG GTGGCACTGC CCGCCCCCCT GCTCCTCACC
    CAGCTGCCCC TGCTCCAGCC CCGGTACTAC TCGGTCAGCT CAGCACCCAG TGCCCACCCA
    GGAGAGATCC ACCTCACAGT AGCTGTGCTG GCATACAGGA CACAGGATGG ACTGGGCCCC
    CTTCATTATG GAGTCTGCTC CACATGGCTA AGCCAACTTA AGGCCGGAGA TCCTGTACCC
    TGCTTCATCA GGGGGGCTCC CTCCTTCCGG CTGCCACCTG ATCCCAGTCT GCCTTGCATC
    TTAGTGGGTC CTGGTACGGG TATTGCCCCC TTTCGGGGTT TCTGGCAGGA GCGGCTGCAC
    GACATTGACA GCAAAGGGCT GCAGCCCGCC CCCATGACTC TGGTGTTCGG CTGCCGATGC
    TCCCAGCTCG ACCACCTTTA CCGCGACGAG GTGCAGGATG CCCAGCAGCG CGGAGTTTTT
    GGGCGCGTCC TCACCGCCTT CTCGCGGGAG CCCGACAGCC CTAAGACGTA CGTGCAGGAC
    ATCCTGCGGA CGGAGCTGGC TGCTGAGGTG CACCGCGTGC TGTGCCTCGA GCGGGGCCAC
    ATGTTTGTGT GCGGCGACGT CACCATGGCA ACCAGCGTCC TGCAGACGGT GCAGCGAATC
    CTGGCGACGG AGGGCGACAT GGAGCTGGAC GAGGCCGGCG ACGTCATCGG CGTGCTGCGG
    GATCAGCAAC GCTATCACGA GGACATTTTC GGGCTCACAC TGCGCACCCA GGAGGTGACA
    AGCCGCATAC GCACCCAGAG CTTCTCCTTG CAGGAGCGGC ATCTGCGGGG CGCGGTGCCC
    TGGGCGTTGG ACCCGCCCGG CCCAGACACC GCCGGCCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17873
    Clone ID Related Accession (Same CDS sequence) XM_005629459.2 , XM_005629459.3
    Accession Version XM_005629459.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGAT
    CTCCCCTGTA CACCATCACC AGGTGGCCCA CCCCCCAGCT GGGTTCGGGA CCCTCGCCTG
    CCCCCATGCA CACTGCGCCA GGCTCTCACC TTCTTCCTGG ACATCACTTC CCCGCCCAGC
    CCTCAACTCC TTCGACTGCT CAGCACCCTG GCTGAAGAGT CCAGTGAACA GCAGGAGCTT
    GAGAGCCTCA GCCAGGACCC CCGGCGCTAT GAGGAATGGA AGTGGTTCCG CTGCCCCACA
    CTGCTGGAGG TACTGGAACA GTTCCCATCG GTGGCACTGC CCGCCCCCCT GCTCCTCACC
    CAGCTGCCCC TGCTCCAGCC CCGGTACTAC TCGGTCAGCT CAGCACCCAG TGCCCACCCA
    GGAGAGATCC ACCTCACAGT AGCTGTGCTG GCATACAGGA CACAGGATGG ACTGGGCCCC
    CTTCATTATG GAGTCTGCTC CACATGGCTA AGCCAACTTA AGGCCGGAGA TCCTGTACCC
    TGCTTCATCA GGGGGGCTCC CTCCTTCCGG CTGCCACCTG ATCCCAGTCT GCCTTGCATC
    TTAGTGGGTC CTGGTACGGG TATTGCCCCC TTTCGGGGTT TCTGGCAGGA GCGGCTGCAC
    GACATTGACA GCAAAGGGCT GCAGCCCGCC CCCATGACTC TGGTGTTCGG CTGCCGATGC
    TCCCAGCTCG ACCACCTTTA CCGCGACGAG GTGCAGGATG CCCAGCAGCG CGGAGTTTTT
    GGGCGCGTCC TCACCGCCTT CTCGCGGGAG CCCGACAGCC CTAAGACGTA CGTGCAGGAC
    ATCCTGCGGA CGGAGCTGGC TGCTGAGGTG CACCGCGTGC TGTGCCTCGA GCGGGGCCAC
    ATGTTTGTGT GCGGCGACGT CACCATGGCA ACCAGCGTCC TGCAGACGGT GCAGCGAATC
    CTGGCGACGG AGGGCGACAT GGAGCTGGAC GAGGCCGGCG ACGTCATCGG CGTGCTGCGG
    GATCAGCAAC GCTATCACGA GGACATTTTC GGGCTCACAC TGCGCACCCA GGAGGTGACA
    AGCCGCATAC GCACCCAGAG CTTCTCCTTG CAGGAGCGGC ATCTGCGGGG CGCGGTGCCC
    TGGGCGTTGG ACCCGCCCGG CCCAGACACC GCCGGCCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629516.1
    CDS78..3719
    Translation

    Target ORF information:

    RefSeq Version XM_005629459.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629459.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGAT
    CTCCCCTGTA CACCATCACC AGGTGGCCCA CCCCCCAGCT GGGTTCGGGA CCCTCGCCTG
    CCCCCATGCA CACTGCGCCA GGCTCTCACC TTCTTCCTGG ACATCACTTC CCCGCCCAGC
    CCTCAACTCC TTCGACTGCT CAGCACCCTG GCTGAAGAGT CCAGTGAACA GCAGGAGCTT
    GAGAGCCTCA GCCAGGACCC CCGGCGCTAT GAGGAATGGA AGTGGTTCCG CTGCCCCACA
    CTGCTGGAGG TACTGGAACA GTTCCCATCG GTGGCACTGC CCGCCCCCCT GCTCCTCACC
    CAGCTGCCCC TGCTCCAGCC CCGGTACTAC TCGGTCAGCT CAGCACCCAG TGCCCACCCA
    GGAGAGATCC ACCTCACAGT AGCTGTGCTG GCATACAGGA CACAGGATGG ACTGGGCCCC
    CTTCATTATG GAGTCTGCTC CACATGGCTA AGCCAACTTA AGGCCGGAGA TCCTGTACCC
    TGCTTCATCA GGGGGGCTCC CTCCTTCCGG CTGCCACCTG ATCCCAGTCT GCCTTGCATC
    TTAGTGGGTC CTGGTACGGG TATTGCCCCC TTTCGGGGTT TCTGGCAGGA GCGGCTGCAC
    GACATTGACA GCAAAGGGCT GCAGCCCGCC CCCATGACTC TGGTGTTCGG CTGCCGATGC
    TCCCAGCTCG ACCACCTTTA CCGCGACGAG GTGCAGGATG CCCAGCAGCG CGGAGTTTTT
    GGGCGCGTCC TCACCGCCTT CTCGCGGGAG CCCGACAGCC CTAAGACGTA CGTGCAGGAC
    ATCCTGCGGA CGGAGCTGGC TGCTGAGGTG CACCGCGTGC TGTGCCTCGA GCGGGGCCAC
    ATGTTTGTGT GCGGCGACGT CACCATGGCA ACCAGCGTCC TGCAGACGGT GCAGCGAATC
    CTGGCGACGG AGGGCGACAT GGAGCTGGAC GAGGCCGGCG ACGTCATCGG CGTGCTGCGG
    GATCAGCAAC GCTATCACGA GGACATTTTC GGGCTCACAC TGCGCACCCA GGAGGTGACA
    AGCCGCATAC GCACCCAGAG CTTCTCCTTG CAGGAGCGGC ATCTGCGGGG CGCGGTGCCC
    TGGGCGTTGG ACCCGCCCGG CCCAGACACC GCCGGCCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17874
    Clone ID Related Accession (Same CDS sequence) XM_005629460.3 , XM_005629460.2
    Accession Version XM_005629460.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, endothelial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGATCTC
    CCCTGTACAC CATCACCAGG TGGCCCACCC CCCAGCTGGG TTCGGGACCC TCGCCTGCCC
    CCATGCACAC TGCGCCAGGC TCTCACCTTC TTCCTGGACA TCACTTCCCC GCCCAGCCCT
    CAACTCCTTC GACTGCTCAG CACCCTGGCT GAAGAGTCCA GTGAACAGCA GGAGCTTGAG
    AGCCTCAGCC AGGACCCCCG GCGCTATGAG GAATGGAAGT GGTTCCGCTG CCCCACACTG
    CTGGAGGTAC TGGAACAGTT CCCATCGGTG GCACTGCCCG CCCCCCTGCT CCTCACCCAG
    CTGCCCCTGC TCCAGCCCCG GTACTACTCG GTCAGCTCAG CACCCAGTGC CCACCCAGGA
    GAGATCCACC TCACAGTAGC TGTGCTGGCA TACAGGACAC AGGATGGACT GGGCCCCCTT
    CATTATGGAG TCTGCTCCAC ATGGCTAAGC CAACTTAAGG CCGGAGATCC TGTACCCTGC
    TTCATCAGGG GGGCTCCCTC CTTCCGGCTG CCACCTGATC CCAGTCTGCC TTGCATCTTA
    GTGGGTCCTG GTACGGGTAT TGCCCCCTTT CGGGGTTTCT GGCAGGAGCG GCTGCACGAC
    ATTGACAGCA AAGGGCTGCA GCCCGCCCCC ATGACTCTGG TGTTCGGCTG CCGATGCTCC
    CAGCTCGACC ACCTTTACCG CGACGAGGTG CAGGATGCCC AGCAGCGCGG AGTTTTTGGG
    CGCGTCCTCA CCGCCTTCTC GCGGGAGCCC GACAGCCCTA AGACGTACGT GCAGGACATC
    CTGCGGACGG AGCTGGCTGC TGAGGTGCAC CGCGTGCTGT GCCTCGAGCG GGGCCACATG
    TTTGTGTGCG GCGACGTCAC CATGGCAACC AGCGTCCTGC AGACGGTGCA GCGAATCCTG
    GCGACGGAGG GCGACATGGA GCTGGACGAG GCCGGCGACG TCATCGGCGT GCTGCGGGAT
    CAGCAACGCT ATCACGAGGA CATTTTCGGG CTCACACTGC GCACCCAGGA GGTGACAAGC
    CGCATACGCA CCCAGAGCTT CTCCTTGCAG GAGCGGCATC TGCGGGGCGC GGTGCCCTGG
    GCGTTGGACC CGCCCGGCCC AGACACCGCC GGCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629517.1
    CDS78..3716
    Translation

    Target ORF information:

    RefSeq Version XM_005629460.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGATCTC
    CCCTGTACAC CATCACCAGG TGGCCCACCC CCCAGCTGGG TTCGGGACCC TCGCCTGCCC
    CCATGCACAC TGCGCCAGGC TCTCACCTTC TTCCTGGACA TCACTTCCCC GCCCAGCCCT
    CAACTCCTTC GACTGCTCAG CACCCTGGCT GAAGAGTCCA GTGAACAGCA GGAGCTTGAG
    AGCCTCAGCC AGGACCCCCG GCGCTATGAG GAATGGAAGT GGTTCCGCTG CCCCACACTG
    CTGGAGGTAC TGGAACAGTT CCCATCGGTG GCACTGCCCG CCCCCCTGCT CCTCACCCAG
    CTGCCCCTGC TCCAGCCCCG GTACTACTCG GTCAGCTCAG CACCCAGTGC CCACCCAGGA
    GAGATCCACC TCACAGTAGC TGTGCTGGCA TACAGGACAC AGGATGGACT GGGCCCCCTT
    CATTATGGAG TCTGCTCCAC ATGGCTAAGC CAACTTAAGG CCGGAGATCC TGTACCCTGC
    TTCATCAGGG GGGCTCCCTC CTTCCGGCTG CCACCTGATC CCAGTCTGCC TTGCATCTTA
    GTGGGTCCTG GTACGGGTAT TGCCCCCTTT CGGGGTTTCT GGCAGGAGCG GCTGCACGAC
    ATTGACAGCA AAGGGCTGCA GCCCGCCCCC ATGACTCTGG TGTTCGGCTG CCGATGCTCC
    CAGCTCGACC ACCTTTACCG CGACGAGGTG CAGGATGCCC AGCAGCGCGG AGTTTTTGGG
    CGCGTCCTCA CCGCCTTCTC GCGGGAGCCC GACAGCCCTA AGACGTACGT GCAGGACATC
    CTGCGGACGG AGCTGGCTGC TGAGGTGCAC CGCGTGCTGT GCCTCGAGCG GGGCCACATG
    TTTGTGTGCG GCGACGTCAC CATGGCAACC AGCGTCCTGC AGACGGTGCA GCGAATCCTG
    GCGACGGAGG GCGACATGGA GCTGGACGAG GCCGGCGACG TCATCGGCGT GCTGCGGGAT
    CAGCAACGCT ATCACGAGGA CATTTTCGGG CTCACACTGC GCACCCAGGA GGTGACAAGC
    CGCATACGCA CCCAGAGCTT CTCCTTGCAG GAGCGGCATC TGCGGGGCGC GGTGCCCTGG
    GCGTTGGACC CGCCCGGCCC AGACACCGCC GGCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17874
    Clone ID Related Accession (Same CDS sequence) XM_005629460.3 , XM_005629460.2
    Accession Version XM_005629460.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, endothelial isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGATCTC
    CCCTGTACAC CATCACCAGG TGGCCCACCC CCCAGCTGGG TTCGGGACCC TCGCCTGCCC
    CCATGCACAC TGCGCCAGGC TCTCACCTTC TTCCTGGACA TCACTTCCCC GCCCAGCCCT
    CAACTCCTTC GACTGCTCAG CACCCTGGCT GAAGAGTCCA GTGAACAGCA GGAGCTTGAG
    AGCCTCAGCC AGGACCCCCG GCGCTATGAG GAATGGAAGT GGTTCCGCTG CCCCACACTG
    CTGGAGGTAC TGGAACAGTT CCCATCGGTG GCACTGCCCG CCCCCCTGCT CCTCACCCAG
    CTGCCCCTGC TCCAGCCCCG GTACTACTCG GTCAGCTCAG CACCCAGTGC CCACCCAGGA
    GAGATCCACC TCACAGTAGC TGTGCTGGCA TACAGGACAC AGGATGGACT GGGCCCCCTT
    CATTATGGAG TCTGCTCCAC ATGGCTAAGC CAACTTAAGG CCGGAGATCC TGTACCCTGC
    TTCATCAGGG GGGCTCCCTC CTTCCGGCTG CCACCTGATC CCAGTCTGCC TTGCATCTTA
    GTGGGTCCTG GTACGGGTAT TGCCCCCTTT CGGGGTTTCT GGCAGGAGCG GCTGCACGAC
    ATTGACAGCA AAGGGCTGCA GCCCGCCCCC ATGACTCTGG TGTTCGGCTG CCGATGCTCC
    CAGCTCGACC ACCTTTACCG CGACGAGGTG CAGGATGCCC AGCAGCGCGG AGTTTTTGGG
    CGCGTCCTCA CCGCCTTCTC GCGGGAGCCC GACAGCCCTA AGACGTACGT GCAGGACATC
    CTGCGGACGG AGCTGGCTGC TGAGGTGCAC CGCGTGCTGT GCCTCGAGCG GGGCCACATG
    TTTGTGTGCG GCGACGTCAC CATGGCAACC AGCGTCCTGC AGACGGTGCA GCGAATCCTG
    GCGACGGAGG GCGACATGGA GCTGGACGAG GCCGGCGACG TCATCGGCGT GCTGCGGGAT
    CAGCAACGCT ATCACGAGGA CATTTTCGGG CTCACACTGC GCACCCAGGA GGTGACAAGC
    CGCATACGCA CCCAGAGCTT CTCCTTGCAG GAGCGGCATC TGCGGGGCGC GGTGCCCTGG
    GCGTTGGACC CGCCCGGCCC AGACACCGCC GGCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629517.1
    CDS73..3711
    Translation

    Target ORF information:

    RefSeq Version XM_005629460.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGATCTC
    CCCTGTACAC CATCACCAGG TGGCCCACCC CCCAGCTGGG TTCGGGACCC TCGCCTGCCC
    CCATGCACAC TGCGCCAGGC TCTCACCTTC TTCCTGGACA TCACTTCCCC GCCCAGCCCT
    CAACTCCTTC GACTGCTCAG CACCCTGGCT GAAGAGTCCA GTGAACAGCA GGAGCTTGAG
    AGCCTCAGCC AGGACCCCCG GCGCTATGAG GAATGGAAGT GGTTCCGCTG CCCCACACTG
    CTGGAGGTAC TGGAACAGTT CCCATCGGTG GCACTGCCCG CCCCCCTGCT CCTCACCCAG
    CTGCCCCTGC TCCAGCCCCG GTACTACTCG GTCAGCTCAG CACCCAGTGC CCACCCAGGA
    GAGATCCACC TCACAGTAGC TGTGCTGGCA TACAGGACAC AGGATGGACT GGGCCCCCTT
    CATTATGGAG TCTGCTCCAC ATGGCTAAGC CAACTTAAGG CCGGAGATCC TGTACCCTGC
    TTCATCAGGG GGGCTCCCTC CTTCCGGCTG CCACCTGATC CCAGTCTGCC TTGCATCTTA
    GTGGGTCCTG GTACGGGTAT TGCCCCCTTT CGGGGTTTCT GGCAGGAGCG GCTGCACGAC
    ATTGACAGCA AAGGGCTGCA GCCCGCCCCC ATGACTCTGG TGTTCGGCTG CCGATGCTCC
    CAGCTCGACC ACCTTTACCG CGACGAGGTG CAGGATGCCC AGCAGCGCGG AGTTTTTGGG
    CGCGTCCTCA CCGCCTTCTC GCGGGAGCCC GACAGCCCTA AGACGTACGT GCAGGACATC
    CTGCGGACGG AGCTGGCTGC TGAGGTGCAC CGCGTGCTGT GCCTCGAGCG GGGCCACATG
    TTTGTGTGCG GCGACGTCAC CATGGCAACC AGCGTCCTGC AGACGGTGCA GCGAATCCTG
    GCGACGGAGG GCGACATGGA GCTGGACGAG GCCGGCGACG TCATCGGCGT GCTGCGGGAT
    CAGCAACGCT ATCACGAGGA CATTTTCGGG CTCACACTGC GCACCCAGGA GGTGACAAGC
    CGCATACGCA CCCAGAGCTT CTCCTTGCAG GAGCGGCATC TGCGGGGCGC GGTGCCCTGG
    GCGTTGGACC CGCCCGGCCC AGACACCGCC GGCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17875
    Clone ID Related Accession (Same CDS sequence) XM_005629461.3 , XM_005629461.2
    Accession Version XM_005629461.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, endothelial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGGTGGC
    CCACCCCCCA GCTGGGTTCG GGACCCTCGC CTGCCCCCAT GCACACTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    CTGGCTGAAG AGTCCAGTGA ACAGCAGGAG CTTGAGAGCC TCAGCCAGGA CCCCCGGCGC
    TATGAGGAAT GGAAGTGGTT CCGCTGCCCC ACACTGCTGG AGGTACTGGA ACAGTTCCCA
    TCGGTGGCAC TGCCCGCCCC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCGGTCA GCTCAGCACC CAGTGCCCAC CCAGGAGAGA TCCACCTCAC AGTAGCTGTG
    CTGGCATACA GGACACAGGA TGGACTGGGC CCCCTTCATT ATGGAGTCTG CTCCACATGG
    CTAAGCCAAC TTAAGGCCGG AGATCCTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCAC CTGATCCCAG TCTGCCTTGC ATCTTAGTGG GTCCTGGTAC GGGTATTGCC
    CCCTTTCGGG GTTTCTGGCA GGAGCGGCTG CACGACATTG ACAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CTCTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGACCACCT TTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGAGTT TTTGGGCGCG TCCTCACCGC CTTCTCGCGG
    GAGCCCGACA GCCCTAAGAC GTACGTGCAG GACATCCTGC GGACGGAGCT GGCTGCTGAG
    GTGCACCGCG TGCTGTGCCT CGAGCGGGGC CACATGTTTG TGTGCGGCGA CGTCACCATG
    GCAACCAGCG TCCTGCAGAC GGTGCAGCGA ATCCTGGCGA CGGAGGGCGA CATGGAGCTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CACTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCTTCTCC
    TTGCAGGAGC GGCATCTGCG GGGCGCGGTG CCCTGGGCGT TGGACCCGCC CGGCCCAGAC
    ACCGCCGGCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629518.1
    CDS1..3615
    Translation

    Target ORF information:

    RefSeq Version XM_005629461.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629461.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGGTGGC
    CCACCCCCCA GCTGGGTTCG GGACCCTCGC CTGCCCCCAT GCACACTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    CTGGCTGAAG AGTCCAGTGA ACAGCAGGAG CTTGAGAGCC TCAGCCAGGA CCCCCGGCGC
    TATGAGGAAT GGAAGTGGTT CCGCTGCCCC ACACTGCTGG AGGTACTGGA ACAGTTCCCA
    TCGGTGGCAC TGCCCGCCCC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCGGTCA GCTCAGCACC CAGTGCCCAC CCAGGAGAGA TCCACCTCAC AGTAGCTGTG
    CTGGCATACA GGACACAGGA TGGACTGGGC CCCCTTCATT ATGGAGTCTG CTCCACATGG
    CTAAGCCAAC TTAAGGCCGG AGATCCTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCAC CTGATCCCAG TCTGCCTTGC ATCTTAGTGG GTCCTGGTAC GGGTATTGCC
    CCCTTTCGGG GTTTCTGGCA GGAGCGGCTG CACGACATTG ACAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CTCTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGACCACCT TTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGAGTT TTTGGGCGCG TCCTCACCGC CTTCTCGCGG
    GAGCCCGACA GCCCTAAGAC GTACGTGCAG GACATCCTGC GGACGGAGCT GGCTGCTGAG
    GTGCACCGCG TGCTGTGCCT CGAGCGGGGC CACATGTTTG TGTGCGGCGA CGTCACCATG
    GCAACCAGCG TCCTGCAGAC GGTGCAGCGA ATCCTGGCGA CGGAGGGCGA CATGGAGCTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CACTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCTTCTCC
    TTGCAGGAGC GGCATCTGCG GGGCGCGGTG CCCTGGGCGT TGGACCCGCC CGGCCCAGAC
    ACCGCCGGCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17875
    Clone ID Related Accession (Same CDS sequence) XM_005629461.3 , XM_005629461.2
    Accession Version XM_005629461.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, endothelial isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGGTGGC
    CCACCCCCCA GCTGGGTTCG GGACCCTCGC CTGCCCCCAT GCACACTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    CTGGCTGAAG AGTCCAGTGA ACAGCAGGAG CTTGAGAGCC TCAGCCAGGA CCCCCGGCGC
    TATGAGGAAT GGAAGTGGTT CCGCTGCCCC ACACTGCTGG AGGTACTGGA ACAGTTCCCA
    TCGGTGGCAC TGCCCGCCCC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCGGTCA GCTCAGCACC CAGTGCCCAC CCAGGAGAGA TCCACCTCAC AGTAGCTGTG
    CTGGCATACA GGACACAGGA TGGACTGGGC CCCCTTCATT ATGGAGTCTG CTCCACATGG
    CTAAGCCAAC TTAAGGCCGG AGATCCTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCAC CTGATCCCAG TCTGCCTTGC ATCTTAGTGG GTCCTGGTAC GGGTATTGCC
    CCCTTTCGGG GTTTCTGGCA GGAGCGGCTG CACGACATTG ACAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CTCTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGACCACCT TTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGAGTT TTTGGGCGCG TCCTCACCGC CTTCTCGCGG
    GAGCCCGACA GCCCTAAGAC GTACGTGCAG GACATCCTGC GGACGGAGCT GGCTGCTGAG
    GTGCACCGCG TGCTGTGCCT CGAGCGGGGC CACATGTTTG TGTGCGGCGA CGTCACCATG
    GCAACCAGCG TCCTGCAGAC GGTGCAGCGA ATCCTGGCGA CGGAGGGCGA CATGGAGCTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CACTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCTTCTCC
    TTGCAGGAGC GGCATCTGCG GGGCGCGGTG CCCTGGGCGT TGGACCCGCC CGGCCCAGAC
    ACCGCCGGCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629518.1
    CDS73..3687
    Translation

    Target ORF information:

    RefSeq Version XM_005629461.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CCCCCCACTC
    ACCCGGCCTC CAGATGGGCC CAAGTTCCCT CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTATGACA CCTTGAGTGC CCAGTCACAG CAGGATGGGC CCTGCACCCC AAGACGCTGT
    CTAGGCTCTC TGGTATTTCC ACGGAAACTA CAGAGCCGGC CCTCCCAGAA CCCTGCACCC
    CCTGAGCAGC TGCTGAGCCA GGCCAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGTGGCT CCCAGGCCCA TGAGCAGAGG CTTCAAGAGG TGGAAGCTGA GGTAGCAGCC
    ACAGGCACCT ATCAGCTTCG GGAAAGCGAG CTGGTGTTCG GGGCCAAGCA GGCCTGGCGA
    AACGCTCCTC GCTGCGTGGG CCGGATCCAG TGGGGGAAGC TGCAGGTGTT TGATGCCCGG
    GATTGCAGCT CTGCCCAGGA GATGTTCACC TACATCTGCA ACCACATCAA GTATGCCACC
    AACCGGGGCA ACCTCCGCTC GGCCATCACA GTGTTCCCCC AGCGTGCCTC AGGCCGCGGA
    GACTTTCGAA TTTGGAACAG CCAGCTGGTG CGCTATGCGG GCTACAGGCA GCAGGATGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACTGAGC TCTGCATCCA GCATGGCTGG
    ACTCCAGGAA ACGGCCGCTT TGATGTGCTG CCCCTACTGC TCCAGGCCCC AGATGAACCC
    CCAGAACTCT TTGCTCTGCC CCCCGAGCTG GTTCTCGAGG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TTGCGGCCCT GGGCCTGCGC TGGTATGCCC TCCCAGCGGT GAGCAACATG
    CTGCTGGAAA TTGGGGGGCT GGAGTTCCCT GCGGCCCCCT TCAGCGGCTG GTACATGAGC
    ACTGAGATTG GCACGCGGAA CCTGTGTGAC CCTCATCGAT ACAACATCCT GGAGGATGTG
    GCTGTCTGCA TGGACTTGGA TACCAGGACC ACCTCATCCC TGTGGAAAGA CAAGGCAGCA
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATCGTGGAC
    CACCATGCTG CCACAGCCTC CTTCATGAAG CACCTGGAGA ACGAGCAGAA GGCCAGGGGG
    GGTTGCCCTG CTGACTGGGC CTGGATCGTG CCCCCCATCT CTGGCAGCCT CACCCCTGTT
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCGGCCT TCCGCTATCA GACAGACCCG
    TGGAAGGGGA GTGCATCCAA GGGTGCCGGC GTCACCAGGA AGAAAACCTT TAAGGAAGTG
    GCCAATGCGG TGAAGATCTC TGCCTCACTC ATGGGCACTG TGATGGCAAA GCGAGTGAAG
    GCGACAATCC TCTATGGCTC CGAGACTGGC CGGGCCCAGA GCTATGCACA GCAGCTGGGG
    AGACTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGACGTGGTG
    TCCCTTGAGC ATGAGACGCT GGTGTTGGTG GTGACCAGCA CATTTGGGAA TGGTGATCCC
    CCAGAGAATG GAGAGAGTTT TGCGGCAGCC CTGATGGAGA TGTCGGGTCC CTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGTGT CTCCTGCTCA
    GACCCACTGG TGTCCTCCTG GAGGCGGAAG AGAAAGGAGT CCAGTAACAC AGACAGTGCT
    GGGGCACTGG GTACCCTCAG GTTCTGTGTG TTCGGGCTGG GCTCCCGGGC ATACCCCCAC
    TTTTGTGCCT TTGCTCGTGC GGTGGACACA CGGCTGGAAG AGCTGGGTGG GGAGCGGCTG
    CTGCAGCTGG GCCAAGGAGA TGAGCTGTGT GGCCAGGAGG AGGCCTTCCG AGGCTGGGCC
    CAGGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCC
    GCCCGGGACA TATTCAGCCC CAAACGGACC TGGAAACGCC AGAGGTACCG GCTGAGCGCC
    CAGGCTGAGG GCCTCCAGCT GCTGCCAGGA CTGATCCATG TGCACAGGAG GAAGATGGTC
    CAGGCTACTG TCCTCGCAGT GGAAAACCTG CAAAGCAGCA AGTCCACCCG GGCCACAATC
    CTGGTGCGCC TGGACACTGG AAGCCAGGAG GCGCTGCAGT ACCAGCCAGG GGACCACATT
    GGCATCTGCC CGCCCAACCG GCCTGGCCTT GTGGAGGCGC TGCTGAGCCG GGTGGAGGAT
    CCACCGCCAC CAGGAGAGCC AGTGGCAGTG GAGCAGCTGG AGAAGGGCAG CCCCGGTGGC
    CCACCCCCCA GCTGGGTTCG GGACCCTCGC CTGCCCCCAT GCACACTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    CTGGCTGAAG AGTCCAGTGA ACAGCAGGAG CTTGAGAGCC TCAGCCAGGA CCCCCGGCGC
    TATGAGGAAT GGAAGTGGTT CCGCTGCCCC ACACTGCTGG AGGTACTGGA ACAGTTCCCA
    TCGGTGGCAC TGCCCGCCCC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCGGTCA GCTCAGCACC CAGTGCCCAC CCAGGAGAGA TCCACCTCAC AGTAGCTGTG
    CTGGCATACA GGACACAGGA TGGACTGGGC CCCCTTCATT ATGGAGTCTG CTCCACATGG
    CTAAGCCAAC TTAAGGCCGG AGATCCTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCAC CTGATCCCAG TCTGCCTTGC ATCTTAGTGG GTCCTGGTAC GGGTATTGCC
    CCCTTTCGGG GTTTCTGGCA GGAGCGGCTG CACGACATTG ACAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CTCTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGACCACCT TTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGAGTT TTTGGGCGCG TCCTCACCGC CTTCTCGCGG
    GAGCCCGACA GCCCTAAGAC GTACGTGCAG GACATCCTGC GGACGGAGCT GGCTGCTGAG
    GTGCACCGCG TGCTGTGCCT CGAGCGGGGC CACATGTTTG TGTGCGGCGA CGTCACCATG
    GCAACCAGCG TCCTGCAGAC GGTGCAGCGA ATCCTGGCGA CGGAGGGCGA CATGGAGCTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CACTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCTTCTCC
    TTGCAGGAGC GGCATCTGCG GGGCGCGGTG CCCTGGGCGT TGGACCCGCC CGGCCCAGAC
    ACCGCCGGCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17876
    Clone ID Related Accession (Same CDS sequence) XM_005629462.2
    Accession Version XM_005629462.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, endothelial isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGAT
    CTCCCCTGTA CACCATCACC AGGTGGCCCA CCCCCCAGCT GGGTTCGGGA CCCTCGCCTG
    CCCCCATGCA CACTGCGCCA GGCTCTCACC TTCTTCCTGG ACATCACTTC CCCGCCCAGC
    CCTCAACTCC TTCGACTGCT CAGCACCCTG GCTGAAGAGT CCAGTGAACA GCAGGAGCTT
    GAGAGCCTCA GCCAGGACCC CCGGCGCTAT GAGGAATGGA AGTGGTTCCG CTGCCCCACA
    CTGCTGGAGG TACTGGAACA GTTCCCATCG GTGGCACTGC CCGCCCCCCT GCTCCTCACC
    CAGCTGCCCC TGCTCCAGCC CCGGTACTAC TCGGTCAGCT CAGCACCCAG TGCCCACCCA
    GGAGAGATCC ACCTCACAGT AGCTGTGCTG GCATACAGGA CACAGGATGG ACTGGGCCCC
    CTTCATTATG GAGTCTGCTC CACATGGCTA AGCCAACTTA AGGCCGGAGA TCCTGTACCC
    TGCTTCATCA GGGGGGCTCC CTCCTTCCGG CTGCCACCTG ATCCCAGTCT GCCTTGCATC
    TTAGTGGGTC CTGGTACGGG TATTGCCCCC TTTCGGGGTT TCTGGCAGGA GCGGCTGCAC
    GACATTGACA GCAAAGGGCT GCAGCCCGCC CCCATGACTC TGGTGTTCGG CTGCCGATGC
    TCCCAGCTCG ACCACCTTTA CCGCGACGAG GTGCAGGATG CCCAGCAGCG CGGAGTTTTT
    GGGCGCGTCC TCACCGCCTT CTCGCGGGAG CCCGACAGCC CTAAGGGCAC GCCCCTAACC
    CCGGCTTCCC TCAGACGTAC GTGCAGGACA TCCTGCGGAC GGAGCTGGCT GCTGAGGTGC
    ACCGCGTGCT GTGCCTCGAG CGGGGCCACA TGTTTGTGTG CGGCGACGTC ACCATGGCAA
    CCAGCGTCCT GCAGACGGTG CAGCGAATCC TGGCGACGGA GGGCGACATG GAGCTGGACG
    AGGCCGGCGA CGTCATCGGC GTGCTGCGGG ATCAGCAACG CTATCACGAG GACATTTTCG
    GGCTCACACT GCGCACCCAG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629519.1
    CDS73..3639
    Translation

    Target ORF information:

    RefSeq Version XM_005629462.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629462.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC 
    CTGGGCCTGG GGCTATGTGG CAAGCAGGGC CCTGCCTCCC CGACCTCAGA GCCCAGCCGG
    GCACCGGCCC TGGCGCCCCC ACCCTCGCCC CCACCAGCAC CAGACCACAG CAGCCCCCCA
    CTCACCCGGC CTCCAGATGG GCCCAAGTTC CCTCGTGTGA AGAACTGGGA GGTGGGGAGC
    ATCACCTATG ACACCTTGAG TGCCCAGTCA CAGCAGGATG GGCCCTGCAC CCCAAGACGC
    TGTCTAGGCT CTCTGGTATT TCCACGGAAA CTACAGAGCC GGCCCTCCCA GAACCCTGCA
    CCCCCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC CCATGAGCAG AGGCTTCAAG AGGTGGAAGC TGAGGTAGCA
    GCCACAGGCA CCTATCAGCT TCGGGAAAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGAAACGCTC CTCGCTGCGT GGGCCGGATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGATTGCA GCTCTGCCCA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTATGCC
    ACCAACCGGG GCAACCTCCG CTCGGCCATC ACAGTGTTCC CCCAGCGTGC CTCAGGCCGC
    GGAGACTTTC GAATTTGGAA CAGCCAGCTG GTGCGCTATG CGGGCTACAG GCAGCAGGAT
    GGCTCTGTGC GGGGGGACCC AGCCAACGTG GAGATCACTG AGCTCTGCAT CCAGCATGGC
    TGGACTCCAG GAAACGGCCG CTTTGATGTG CTGCCCCTAC TGCTCCAGGC CCCAGATGAA
    CCCCCAGAAC TCTTTGCTCT GCCCCCCGAG CTGGTTCTCG AGGTGCCCCT GGAGCACCCC
    ACGCTGGAGT GGTTTGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCAGC GGTGAGCAAC
    ATGCTGCTGG AAATTGGGGG GCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACTGAGA TTGGCACGCG GAACCTGTGT GACCCTCATC GATACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACTT GGATACCAGG ACCACCTCAT CCCTGTGGAA AGACAAGGCA
    GCAGTGGAAA TCAACTTGGC TGTGCTGCAC AGTTACCAGC TGGCCAAAGT GACCATCGTG
    GACCACCATG CTGCCACAGC CTCCTTCATG AAGCACCTGG AGAACGAGCA GAAGGCCAGG
    GGGGGTTGCC CTGCTGACTG GGCCTGGATC GTGCCCCCCA TCTCTGGCAG CCTCACCCCT
    GTTTTCCATC AGGAGATGGT CAACTATGTC CTGTCCCCGG CCTTCCGCTA TCAGACAGAC
    CCGTGGAAGG GGAGTGCATC CAAGGGTGCC GGCGTCACCA GGAAGAAAAC CTTTAAGGAA
    GTGGCCAATG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CTGTGATGGC AAAGCGAGTG
    AAGGCGACAA TCCTCTATGG CTCCGAGACT GGCCGGGCCC AGAGCTATGC ACAGCAGCTG
    GGGAGACTCT TCCGGAAGGC TTTCGACCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTTG AGCATGAGAC GCTGGTGTTG GTGGTGACCA GCACATTTGG GAATGGTGAT
    CCCCCAGAGA ATGGAGAGAG TTTTGCGGCA GCCCTGATGG AGATGTCGGG TCCCTACAAC
    AGCTCCCCTC GGCCAGAACA GCACAAGAGT TACAAAATCC GCTTCAACAG TGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGAGGCGG AAGAGAAAGG AGTCCAGTAA CACAGACAGT
    GCTGGGGCAC TGGGTACCCT CAGGTTCTGT GTGTTCGGGC TGGGCTCCCG GGCATACCCC
    CACTTTTGTG CCTTTGCTCG TGCGGTGGAC ACACGGCTGG AAGAGCTGGG TGGGGAGCGG
    CTGCTGCAGC TGGGCCAAGG AGATGAGCTG TGTGGCCAGG AGGAGGCCTT CCGAGGCTGG
    GCCCAGGCCG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TGGGAGAGGA TGCCAAGGCT
    GCCGCCCGGG ACATATTCAG CCCCAAACGG ACCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCTG AGGGCCTCCA GCTGCTGCCA GGACTGATCC ATGTGCACAG GAGGAAGATG
    GTCCAGGCTA CTGTCCTCGC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACA
    ATCCTGGTGC GCCTGGACAC TGGAAGCCAG GAGGCGCTGC AGTACCAGCC AGGGGACCAC
    ATTGGCATCT GCCCGCCCAA CCGGCCTGGC CTTGTGGAGG CGCTGCTGAG CCGGGTGGAG
    GATCCACCGC CACCAGGAGA GCCAGTGGCA GTGGAGCAGC TGGAGAAGGG CAGCCCCGAT
    CTCCCCTGTA CACCATCACC AGGTGGCCCA CCCCCCAGCT GGGTTCGGGA CCCTCGCCTG
    CCCCCATGCA CACTGCGCCA GGCTCTCACC TTCTTCCTGG ACATCACTTC CCCGCCCAGC
    CCTCAACTCC TTCGACTGCT CAGCACCCTG GCTGAAGAGT CCAGTGAACA GCAGGAGCTT
    GAGAGCCTCA GCCAGGACCC CCGGCGCTAT GAGGAATGGA AGTGGTTCCG CTGCCCCACA
    CTGCTGGAGG TACTGGAACA GTTCCCATCG GTGGCACTGC CCGCCCCCCT GCTCCTCACC
    CAGCTGCCCC TGCTCCAGCC CCGGTACTAC TCGGTCAGCT CAGCACCCAG TGCCCACCCA
    GGAGAGATCC ACCTCACAGT AGCTGTGCTG GCATACAGGA CACAGGATGG ACTGGGCCCC
    CTTCATTATG GAGTCTGCTC CACATGGCTA AGCCAACTTA AGGCCGGAGA TCCTGTACCC
    TGCTTCATCA GGGGGGCTCC CTCCTTCCGG CTGCCACCTG ATCCCAGTCT GCCTTGCATC
    TTAGTGGGTC CTGGTACGGG TATTGCCCCC TTTCGGGGTT TCTGGCAGGA GCGGCTGCAC
    GACATTGACA GCAAAGGGCT GCAGCCCGCC CCCATGACTC TGGTGTTCGG CTGCCGATGC
    TCCCAGCTCG ACCACCTTTA CCGCGACGAG GTGCAGGATG CCCAGCAGCG CGGAGTTTTT
    GGGCGCGTCC TCACCGCCTT CTCGCGGGAG CCCGACAGCC CTAAGGGCAC GCCCCTAACC
    CCGGCTTCCC TCAGACGTAC GTGCAGGACA TCCTGCGGAC GGAGCTGGCT GCTGAGGTGC
    ACCGCGTGCT GTGCCTCGAG CGGGGCCACA TGTTTGTGTG CGGCGACGTC ACCATGGCAA
    CCAGCGTCCT GCAGACGGTG CAGCGAATCC TGGCGACGGA GGGCGACATG GAGCTGGACG
    AGGCCGGCGA CGTCATCGGC GTGCTGCGGG ATCAGCAACG CTATCACGAG GACATTTTCG
    GGCTCACACT GCGCACCCAG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.