gRNAs
The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OCe293770 | |
| Clone ID Related Accession (Same CDS sequence) | XM_022403591.1 | |
| Accession Version | XM_022403591.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nitric oxide synthase, endothelial isoform X4 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGGAGAT GTCCCAGCAA GGAGATAAGG GACAGACCAG ATGTGTGTGG TGCAGGGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_022259299.1 |
| CDS | 749..3892 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_022403591.1 |
1 | ATGGGGAGAT GTCCCAGCAA GGAGATAAGG GACAGACCAG ATGTGTGTGG TGCAGGGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe34317 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001003158.2 | |
| Accession Version | NM_001003158.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nitric oxide synthase, endothelial | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010272.1. On Oct 27, 2011 this sequence version replaced NM_001003158.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF143503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA2240903 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end. | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001003158.2 |
| CDS | 241..3858 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001003158.2 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17873 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629459.2 , XM_005629459.3 | |
| Accession Version | XM_005629459.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | nitric oxide synthase, endothelial isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629516.1 |
| CDS | 73..3714 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629459.2 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17873 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629459.2 , XM_005629459.3 | |
| Accession Version | XM_005629459.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nitric oxide synthase, endothelial isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629516.1 |
| CDS | 78..3719 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629459.3 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17874 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629460.3 , XM_005629460.2 | |
| Accession Version | XM_005629460.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nitric oxide synthase, endothelial isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629517.1 |
| CDS | 78..3716 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629460.3 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17874 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629460.3 , XM_005629460.2 | |
| Accession Version | XM_005629460.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | nitric oxide synthase, endothelial isoform X2 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629517.1 |
| CDS | 73..3711 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629460.2 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17875 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629461.3 , XM_005629461.2 | |
| Accession Version | XM_005629461.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nitric oxide synthase, endothelial isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629518.1 |
| CDS | 1..3615 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629461.3 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17875 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629461.3 , XM_005629461.2 | |
| Accession Version | XM_005629461.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | nitric oxide synthase, endothelial isoform X3 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629518.1 |
| CDS | 73..3687 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629461.2 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe17876 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005629462.2 | |
| Accession Version | XM_005629462.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | nitric oxide synthase, endothelial isoform X4 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005629519.1 |
| CDS | 73..3639 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005629462.2 |
1 | ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CTTGTGGCCT GGGGCTGGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Role of nitric oxide in the coupling of myocardial oxygen consumption and coronary vascular dynamics during pregnancy in the dog. |
Restoration of impaired endothelium-dependent coronary vasodilation in failing heart: role of eNOS phosphorylation and CGMP/cGK-I signaling. |
Effects of nitric oxide synthase inhibition by L-NAME on oxygen uptake kinetics in isolated canine muscle in situ. |
Expression of endothelial nitric oxide synthase in the vascular wall during arteriogenesis. |
Assembly and characterization of canine heart endothelial nitric oxide synthase cDNA and 5'-flanking sequence by homology (RT-)PCR cloning. |
