MDM2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDM2
Entrez Gene ID	403693
Full Name	MDM2 proto-oncogene
General protein information	Preferred Names MDM2 proto-oncogene Names E3 ubiquitin-protein ligase Mdm2 MDM2 alpha Mdm2 p53 binding protein homolog Mdm2, p53 E3 ubiquitin protein ligase homolog RING-type E3 ubiquitin transferase Mdm2 cdm2 double minute 2 homolog double minute 2 protein p53-binding protein Mdm2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005625451.2	XP_005625508.1	E3 ubiquitin-protein ligase Mdm2 isoform X2
NM_001003103.2	NP_001003103.1	E3 ubiquitin-protein ligase Mdm2
XM_022423984.1	XP_022279692.1	E3 ubiquitin-protein ligase Mdm2 isoform X4
XM_005625451.3	XP_005625508.1	E3 ubiquitin-protein ligase Mdm2 isoform X2
XM_005625450.2	XP_005625507.1	E3 ubiquitin-protein ligase Mdm2 isoform X1
XM_022423982.1	XP_022279690.1	E3 ubiquitin-protein ligase Mdm2 isoform X2
XM_005625450.3	XP_005625507.1	E3 ubiquitin-protein ligase Mdm2 isoform X1
XM_022423983.1	XP_022279691.1	E3 ubiquitin-protein ligase Mdm2 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mdm2	NP_001101569.1
Gallus gallus (chicken)	MDM2	NP_001186313.1
Danio rerio (zebrafish)	mdm2	NP_571439.2
Xenopus tropicalis (tropical clawed frog)	mdm2	NP_001231689.1
Mus musculus (house mouse)	Mdm2	NP_034916.1
Pan troglodytes (chimpanzee)	MDM2	XP_001155208.1
	MDM2	XP_001117336.2
Bos taurus (cattle)	MDM2	NP_001092577.1
Homo sapiens (human)	MDM2	NP_002383.2
Canis lupus familiaris (dog)	MDM2	NP_001003103.1

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphism of P53-Ets/AP1 transactivation region of MDM2 oncogene and its immunohistochemical analysis in canine tumours.
Rezaie A, Tabandeh MR, Noori SM
Veterinary and comparative oncology14(2)137-46(2016 Jun)

Cloning, sequence analysis and expression of the cDNAs encoding the canine and equine homologues of the mouse double minute 2 (mdm2) proto-oncogene.
Nasir L, Burr PD, McFarlane ST, Gault E, Thompson H, Argyle DJ
Cancer letters152(1)9-13(2000 Apr)

A novel exon within the mdm2 gene modulates translation initiation in vitro and disrupts the p53-binding domain of mdm2 protein.
Veldhoen N, Metcalfe S, Milner J
Oncogene18(50)7026-33(1999 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tumours that exhibited mutation within the MDM2 promoter had amplification of MDM2 protein levels.
Title: Polymorphism of P53-Ets/AP1 transactivation region of MDM2 oncogene and its immunohistochemical analysis in canine tumours.

The following MDM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00283	NM_001003103.2 Latest version!	Canis lupus familiaris MDM2 proto-oncogene (MDM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe12970	XM_005625450.3 Latest version!	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe12971	XM_005625451.3 Latest version!	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe12971	XM_022423982.1 Latest version!	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe291198	XM_022423983.1 Latest version!	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe291199	XM_022423984.1 Latest version!	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OCe12971	XM_005625451.2	Canis lupus familiaris MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe12970	XM_005625450.2	Canis lupus familiaris MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDM2 ( NM_001003103.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003103.1 Canis lupus familiaris MDM2 proto-oncogene (MDM2), mRNA.

Price & Availability↑

CloneID	OCe00283
Clone ID Related Accession (Same CDS sequence)	NM_001003103.2
Accession Version	NM_001003103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Mdm2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF322416.2. On Feb 21, 2013 this sequence version replaced NM_001003103.1. ##Evidence-Data-START## Transcript exon combination :: AF322416.2, AB031276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AACCATCAGA TTCAGGCACA 
TCTGTGAGTG AAAATAGCTG TCACCGTGAA GGTGGGAGTG ATCAAAAGGA CCCTGTGCAA 
GAGTTGCAGG AAGAGAAACC TTCATCTTCA GATTTGATTT CTAGACCATC TACCTCATCT 
AGAAGGAGAA CAATTAGTGA GACAGAAGAA CATGCAGATG ATTTACCTGG TGAACGGCAG 
AGAAAGCGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT TGCTCTGTGT 
GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AGTCAACAGG GACGCCATCA 
AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCACCATTCA 
GTTTCAGATC AATTCAGTGT AGAATTTGAA GTTGAGTCTC TTGATTCAGA AGATTATAGC 
CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTGTATCG AGTAACTGTG 
TATCAGGCAG GGGAGAGTGA TACAGATTCA TTTGAAGAAG ATCCTGAAAT TTCCTTAGCT 
GACTATTGGA AGTGTACCTC ATGCAATGAA ATGAATCCTC CTCTTCCACC TCATTGCAAT 
AGATGTTGGG CCCTTCGTGA GAATTGGCTT CCTGAAGATA AAGGGAAAAT CCCTGAGAAA 
GCCACACCAG AAAACTCAAC ACAAGTAGAA GAGGGCTTTG ATGTCCCTGA TTGTAAAAAA 
GCTGCAGCCA GTGATTCCAG AGAATCATGT GCTGAGGAAA TTGATGATAA AATCACACAA 
GCCTCCCACT CCCAAGAAAG TGAGGACTAT TCTCAGCCAT CAACTTCTAA CAGCATCATT 
TATAGCAGCC AAGAAGATGT CAAAGAATTT GAGAGAGAAG AAACACAAGA CAAAGAAGAA 
ATTGTGGAAT CTAGTTTTCC CCTTAATGCC ATTGAACCTT GTGTGATTTG CCAAGGTCGA 
CCTAAAAACG GTTGCATTGT TCATGGCAAA ACAGGACATC TTATGGCATG CTTCACGTGT 
GCAAAGAAGC TAAAGAAAAG GAATAAGCCC TGTCCAGTAT GTAGACAACC AATTCAAATG 
ATTGTGCTAA CTTATTTCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003103.1
CDS	7..1470
Translation	MCNTNMSVSTDGAVSTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDHHSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	NM_001003103.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene (MDM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003103.2

ORF Insert Sequence:

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AACCATCAGA TTCAGGCACA 
TCTGTGAGTG AAAATAGCTG TCACCGTGAA GGTGGGAGTG ATCAAAAGGA CCCTGTGCAA 
GAGTTGCAGG AAGAGAAACC TTCATCTTCA GATTTGATTT CTAGACCATC TACCTCATCT 
AGAAGGAGAA CAATTAGTGA GACAGAAGAA CATGCAGATG ATTTACCTGG TGAACGGCAG 
AGAAAGCGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT TGCTCTGTGT 
GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AGTCAACAGG GACGCCATCA 
AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCACCATTCA 
GTTTCAGATC AATTCAGTGT AGAATTTGAA GTTGAGTCTC TTGATTCAGA AGATTATAGC 
CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTGTATCG AGTAACTGTG 
TATCAGGCAG GGGAGAGTGA TACAGATTCA TTTGAAGAAG ATCCTGAAAT TTCCTTAGCT 
GACTATTGGA AGTGTACCTC ATGCAATGAA ATGAATCCTC CTCTTCCACC TCATTGCAAT 
AGATGTTGGG CCCTTCGTGA GAATTGGCTT CCTGAAGATA AAGGGAAAAT CCCTGAGAAA 
GCCACACCAG AAAACTCAAC ACAAGTAGAA GAGGGCTTTG ATGTCCCTGA TTGTAAAAAA 
GCTGCAGCCA GTGATTCCAG AGAATCATGT GCTGAGGAAA TTGATGATAA AATCACACAA 
GCCTCCCACT CCCAAGAAAG TGAGGACTAT TCTCAGCCAT CAACTTCTAA CAGCATCATT 
TATAGCAGCC AAGAAGATGT CAAAGAATTT GAGAGAGAAG AAACACAAGA CAAAGAAGAA 
ATTGTGGAAT CTAGTTTTCC CCTTAATGCC ATTGAACCTT GTGTGATTTG CCAAGGTCGA 
CCTAAAAACG GTTGCATTGT TCATGGCAAA ACAGGACATC TTATGGCATG CTTCACGTGT 
GCAAAGAAGC TAAAGAAAAG GAATAAGCCC TGTCCAGTAT GTAGACAACC AATTCAAATG 
ATTGTGCTAA CTTATTTCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_005625450.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625507.1 Canis lupus familiaris MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12970
Clone ID Related Accession (Same CDS sequence)	XM_005625450.2 , XM_005625450.3
Accession Version	XM_005625450.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AACCATCAGA TTCAGGCACA 
TCTGTGAGTG AAAATAGCTG TCACCGTGAA GGTGGGAGTG ATCAAAAGGA CCCTGTGCAA 
GAGTTGCAGG AAGAGAAACC TTCATCTTCA GATTTGATTT CTAGACCATC TACCTCATCT 
AGAAGGAGAA CAATTAGTGA GACAGAAGAA CATGCAGATG ATTTACCTGG TGAACGGCAG 
AGAAAGCGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT TGCTCTGTGT 
GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AGTCAACAGG GACGCCATCA 
AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCAGGATTCA 
GTTTCAGATC AATTCAGTGT AGAATTTGAA GTTGAGTCTC TTGATTCAGA AGATTATAGC 
CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTGTATCG AGTAACTGTG 
TATCAGGCAG GGGAGAGTGA TACAGATTCA TTTGAAGAAG ATCCTGAAAT TTCCTTAGCT 
GACTATTGGA AGTGTACCTC ATGCAATGAA ATGAATCCTC CTCTTCCACC TCATTGCAAT 
AGATGTTGGG CCCTTCGTGA GAATTGGCTT CCTGAAGATA AAGGGAAAAT CCCTGAGAAA 
GCCACACCAG AAAACTCAAC ACAAGTAGAA GAGGGCTTTG ATGTCCCTGA TTGTAAAAAA 
GCTGCAGCCA GTGATTCCAG AGAATCATGT GCTGAGGAAA TTGATGATAA AATCACACAA 
GCCTCCCACT CCCAAGAAAG TGAGGACTAT TCTCAGCCAT CAACTTCTAA CAGCATCATT 
TATAGCAGCC AAGAAGATGT CAAAGAATTT GAGAGAGAAG AAACACAAGA CAAAGAAGAA 
ATTGTGGAAT CTAGTTTTCC CCTTAATGCC ATTGAACCTT GTGTGATTTG CCAAGGTCGA 
CCTAAAAACG GTTGCATTGT TCATGGCAAA ACAGGACATC TTATGGCATG CTTCACGTGT 
GCAAAGAAGC TAAAGAAAAG GAATAAGCCC TGTCCAGTAT GTAGACAACC AATTCAAATG 
ATTGTGCTAA CTTATTTCCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625507.1
CDS	113..1576
Translation	MCNTNMSVSTDGAVSTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_005625450.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625450.2

ORF Insert Sequence:

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AACCATCAGA TTCAGGCACA 
TCTGTGAGTG AAAATAGCTG TCACCGTGAA GGTGGGAGTG ATCAAAAGGA CCCTGTGCAA 
GAGTTGCAGG AAGAGAAACC TTCATCTTCA GATTTGATTT CTAGACCATC TACCTCATCT 
AGAAGGAGAA CAATTAGTGA GACAGAAGAA CATGCAGATG ATTTACCTGG TGAACGGCAG 
AGAAAGCGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT TGCTCTGTGT 
GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AGTCAACAGG GACGCCATCA 
AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCAGGATTCA 
GTTTCAGATC AATTCAGTGT AGAATTTGAA GTTGAGTCTC TTGATTCAGA AGATTATAGC 
CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTGTATCG AGTAACTGTG 
TATCAGGCAG GGGAGAGTGA TACAGATTCA TTTGAAGAAG ATCCTGAAAT TTCCTTAGCT 
GACTATTGGA AGTGTACCTC ATGCAATGAA ATGAATCCTC CTCTTCCACC TCATTGCAAT 
AGATGTTGGG CCCTTCGTGA GAATTGGCTT CCTGAAGATA AAGGGAAAAT CCCTGAGAAA 
GCCACACCAG AAAACTCAAC ACAAGTAGAA GAGGGCTTTG ATGTCCCTGA TTGTAAAAAA 
GCTGCAGCCA GTGATTCCAG AGAATCATGT GCTGAGGAAA TTGATGATAA AATCACACAA 
GCCTCCCACT CCCAAGAAAG TGAGGACTAT TCTCAGCCAT CAACTTCTAA CAGCATCATT 
TATAGCAGCC AAGAAGATGT CAAAGAATTT GAGAGAGAAG AAACACAAGA CAAAGAAGAA 
ATTGTGGAAT CTAGTTTTCC CCTTAATGCC ATTGAACCTT GTGTGATTTG CCAAGGTCGA 
CCTAAAAACG GTTGCATTGT TCATGGCAAA ACAGGACATC TTATGGCATG CTTCACGTGT 
GCAAAGAAGC TAAAGAAAAG GAATAAGCCC TGTCCAGTAT GTAGACAACC AATTCAAATG 
ATTGTGCTAA CTTATTTCCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_005625450.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625507.1 Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12970
Clone ID Related Accession (Same CDS sequence)	XM_005625450.2 , XM_005625450.3
Accession Version	XM_005625450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AACCATCAGA TTCAGGCACA 
TCTGTGAGTG AAAATAGCTG TCACCGTGAA GGTGGGAGTG ATCAAAAGGA CCCTGTGCAA 
GAGTTGCAGG AAGAGAAACC TTCATCTTCA GATTTGATTT CTAGACCATC TACCTCATCT 
AGAAGGAGAA CAATTAGTGA GACAGAAGAA CATGCAGATG ATTTACCTGG TGAACGGCAG 
AGAAAGCGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT TGCTCTGTGT 
GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AGTCAACAGG GACGCCATCA 
AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCAGGATTCA 
GTTTCAGATC AATTCAGTGT AGAATTTGAA GTTGAGTCTC TTGATTCAGA AGATTATAGC 
CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTGTATCG AGTAACTGTG 
TATCAGGCAG GGGAGAGTGA TACAGATTCA TTTGAAGAAG ATCCTGAAAT TTCCTTAGCT 
GACTATTGGA AGTGTACCTC ATGCAATGAA ATGAATCCTC CTCTTCCACC TCATTGCAAT 
AGATGTTGGG CCCTTCGTGA GAATTGGCTT CCTGAAGATA AAGGGAAAAT CCCTGAGAAA 
GCCACACCAG AAAACTCAAC ACAAGTAGAA GAGGGCTTTG ATGTCCCTGA TTGTAAAAAA 
GCTGCAGCCA GTGATTCCAG AGAATCATGT GCTGAGGAAA TTGATGATAA AATCACACAA 
GCCTCCCACT CCCAAGAAAG TGAGGACTAT TCTCAGCCAT CAACTTCTAA CAGCATCATT 
TATAGCAGCC AAGAAGATGT CAAAGAATTT GAGAGAGAAG AAACACAAGA CAAAGAAGAA 
ATTGTGGAAT CTAGTTTTCC CCTTAATGCC ATTGAACCTT GTGTGATTTG CCAAGGTCGA 
CCTAAAAACG GTTGCATTGT TCATGGCAAA ACAGGACATC TTATGGCATG CTTCACGTGT 
GCAAAGAAGC TAAAGAAAAG GAATAAGCCC TGTCCAGTAT GTAGACAACC AATTCAAATG 
ATTGTGCTAA CTTATTTCCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625507.1
CDS	150..1613
Translation	MCNTNMSVSTDGAVSTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_005625450.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625450.3

ORF Insert Sequence:

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AACCATCAGA TTCAGGCACA 
TCTGTGAGTG AAAATAGCTG TCACCGTGAA GGTGGGAGTG ATCAAAAGGA CCCTGTGCAA 
GAGTTGCAGG AAGAGAAACC TTCATCTTCA GATTTGATTT CTAGACCATC TACCTCATCT 
AGAAGGAGAA CAATTAGTGA GACAGAAGAA CATGCAGATG ATTTACCTGG TGAACGGCAG 
AGAAAGCGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT TGCTCTGTGT 
GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AGTCAACAGG GACGCCATCA 
AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCAGGATTCA 
GTTTCAGATC AATTCAGTGT AGAATTTGAA GTTGAGTCTC TTGATTCAGA AGATTATAGC 
CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTGTATCG AGTAACTGTG 
TATCAGGCAG GGGAGAGTGA TACAGATTCA TTTGAAGAAG ATCCTGAAAT TTCCTTAGCT 
GACTATTGGA AGTGTACCTC ATGCAATGAA ATGAATCCTC CTCTTCCACC TCATTGCAAT 
AGATGTTGGG CCCTTCGTGA GAATTGGCTT CCTGAAGATA AAGGGAAAAT CCCTGAGAAA 
GCCACACCAG AAAACTCAAC ACAAGTAGAA GAGGGCTTTG ATGTCCCTGA TTGTAAAAAA 
GCTGCAGCCA GTGATTCCAG AGAATCATGT GCTGAGGAAA TTGATGATAA AATCACACAA 
GCCTCCCACT CCCAAGAAAG TGAGGACTAT TCTCAGCCAT CAACTTCTAA CAGCATCATT 
TATAGCAGCC AAGAAGATGT CAAAGAATTT GAGAGAGAAG AAACACAAGA CAAAGAAGAA 
ATTGTGGAAT CTAGTTTTCC CCTTAATGCC ATTGAACCTT GTGTGATTTG CCAAGGTCGA 
CCTAAAAACG GTTGCATTGT TCATGGCAAA ACAGGACATC TTATGGCATG CTTCACGTGT 
GCAAAGAAGC TAAAGAAAAG GAATAAGCCC TGTCCAGTAT GTAGACAACC AATTCAAATG 
ATTGTGCTAA CTTATTTCCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_005625451.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625508.1 Canis lupus familiaris MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe12971
Clone ID Related Accession (Same CDS sequence)	XM_005625451.2 , XM_005625451.3 , XM_022423982.1
Accession Version	XM_005625451.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGG TGATATTTTA TCTTGGCCAG TATATTATGA CTAAACGATT GTATGATGAG 
AAGCAACAGC ATATTGTGTA TTGTTCCAAT GATCTTCTAG GAGATTTGTT TGGAGTGCCA 
AGCTTCTCTG TGAAAGAGCA CAGGAAAATA TATACAATGA TCTACAGAAA CTTGGTAGTA 
GTCAATCAGC ATGAACCATC AGATTCAGGC ACATCTGTGA GTGAAAATAG CTGTCACCGT 
GAAGGTGGGA GTGATCAAAA GGACCCTGTG CAAGAGTTGC AGGAAGAGAA ACCTTCATCT 
TCAGATTTGA TTTCTAGACC ATCTACCTCA TCTAGAAGGA GAACAATTAG TGAGACAGAA 
GAACATGCAG ATGATTTACC TGGTGAACGG CAGAGAAAGC GCCACAAATC TGATAGTATT 
TCCCTTTCCT TTGATGAAAG CCTTGCTCTG TGTGTAATAA GGGAGATATG TTGTGAAAGA 
AGCAGTAGCA GTGAGTCAAC AGGGACGCCA TCAAATCCGG ATCTTGATGC TGGTGTAAGT 
GAACATTCAG GTGATTGGTT GGATCAGGAT TCAGTTTCAG ATCAATTCAG TGTAGAATTT 
GAAGTTGAGT CTCTTGATTC AGAAGATTAT AGCCTTAGTG AAGAAGGACA AGAACTCTCA 
GATGAAGATG ATGAGGTGTA TCGAGTAACT GTGTATCAGG CAGGGGAGAG TGATACAGAT 
TCATTTGAAG AAGATCCTGA AATTTCCTTA GCTGACTATT GGAAGTGTAC CTCATGCAAT 
GAAATGAATC CTCCTCTTCC ACCTCATTGC AATAGATGTT GGGCCCTTCG TGAGAATTGG 
CTTCCTGAAG ATAAAGGGAA AATCCCTGAG AAAGCCACAC CAGAAAACTC AACACAAGTA 
GAAGAGGGCT TTGATGTCCC TGATTGTAAA AAAGCTGCAG CCAGTGATTC CAGAGAATCA 
TGTGCTGAGG AAATTGATGA TAAAATCACA CAAGCCTCCC ACTCCCAAGA AAGTGAGGAC 
TATTCTCAGC CATCAACTTC TAACAGCATC ATTTATAGCA GCCAAGAAGA TGTCAAAGAA 
TTTGAGAGAG AAGAAACACA AGACAAAGAA GAAATTGTGG AATCTAGTTT TCCCCTTAAT 
GCCATTGAAC CTTGTGTGAT TTGCCAAGGT CGACCTAAAA ACGGTTGCAT TGTTCATGGC 
AAAACAGGAC ATCTTATGGC ATGCTTCACG TGTGCAAAGA AGCTAAAGAA AAGGAATAAG 
CCCTGTCCAG TATGTAGACA ACCAATTCAA ATGATTGTGC TAACTTATTT CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625508.1
CDS	173..1489
Translation	MKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_005625451.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625451.2

ORF Insert Sequence:

ATGAAAGAGG TGATATTTTA TCTTGGCCAG TATATTATGA CTAAACGATT GTATGATGAG 
AAGCAACAGC ATATTGTGTA TTGTTCCAAT GATCTTCTAG GAGATTTGTT TGGAGTGCCA 
AGCTTCTCTG TGAAAGAGCA CAGGAAAATA TATACAATGA TCTACAGAAA CTTGGTAGTA 
GTCAATCAGC ATGAACCATC AGATTCAGGC ACATCTGTGA GTGAAAATAG CTGTCACCGT 
GAAGGTGGGA GTGATCAAAA GGACCCTGTG CAAGAGTTGC AGGAAGAGAA ACCTTCATCT 
TCAGATTTGA TTTCTAGACC ATCTACCTCA TCTAGAAGGA GAACAATTAG TGAGACAGAA 
GAACATGCAG ATGATTTACC TGGTGAACGG CAGAGAAAGC GCCACAAATC TGATAGTATT 
TCCCTTTCCT TTGATGAAAG CCTTGCTCTG TGTGTAATAA GGGAGATATG TTGTGAAAGA 
AGCAGTAGCA GTGAGTCAAC AGGGACGCCA TCAAATCCGG ATCTTGATGC TGGTGTAAGT 
GAACATTCAG GTGATTGGTT GGATCAGGAT TCAGTTTCAG ATCAATTCAG TGTAGAATTT 
GAAGTTGAGT CTCTTGATTC AGAAGATTAT AGCCTTAGTG AAGAAGGACA AGAACTCTCA 
GATGAAGATG ATGAGGTGTA TCGAGTAACT GTGTATCAGG CAGGGGAGAG TGATACAGAT 
TCATTTGAAG AAGATCCTGA AATTTCCTTA GCTGACTATT GGAAGTGTAC CTCATGCAAT 
GAAATGAATC CTCCTCTTCC ACCTCATTGC AATAGATGTT GGGCCCTTCG TGAGAATTGG 
CTTCCTGAAG ATAAAGGGAA AATCCCTGAG AAAGCCACAC CAGAAAACTC AACACAAGTA 
GAAGAGGGCT TTGATGTCCC TGATTGTAAA AAAGCTGCAG CCAGTGATTC CAGAGAATCA 
TGTGCTGAGG AAATTGATGA TAAAATCACA CAAGCCTCCC ACTCCCAAGA AAGTGAGGAC 
TATTCTCAGC CATCAACTTC TAACAGCATC ATTTATAGCA GCCAAGAAGA TGTCAAAGAA 
TTTGAGAGAG AAGAAACACA AGACAAAGAA GAAATTGTGG AATCTAGTTT TCCCCTTAAT 
GCCATTGAAC CTTGTGTGAT TTGCCAAGGT CGACCTAAAA ACGGTTGCAT TGTTCATGGC 
AAAACAGGAC ATCTTATGGC ATGCTTCACG TGTGCAAAGA AGCTAAAGAA AAGGAATAAG 
CCCTGTCCAG TATGTAGACA ACCAATTCAA ATGATTGTGC TAACTTATTT CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_005625451.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625508.1 Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe12971
Clone ID Related Accession (Same CDS sequence)	XM_005625451.2 , XM_005625451.3 , XM_022423982.1
Accession Version	XM_005625451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGG TGATATTTTA TCTTGGCCAG TATATTATGA CTAAACGATT GTATGATGAG 
AAGCAACAGC ATATTGTGTA TTGTTCCAAT GATCTTCTAG GAGATTTGTT TGGAGTGCCA 
AGCTTCTCTG TGAAAGAGCA CAGGAAAATA TATACAATGA TCTACAGAAA CTTGGTAGTA 
GTCAATCAGC ATGAACCATC AGATTCAGGC ACATCTGTGA GTGAAAATAG CTGTCACCGT 
GAAGGTGGGA GTGATCAAAA GGACCCTGTG CAAGAGTTGC AGGAAGAGAA ACCTTCATCT 
TCAGATTTGA TTTCTAGACC ATCTACCTCA TCTAGAAGGA GAACAATTAG TGAGACAGAA 
GAACATGCAG ATGATTTACC TGGTGAACGG CAGAGAAAGC GCCACAAATC TGATAGTATT 
TCCCTTTCCT TTGATGAAAG CCTTGCTCTG TGTGTAATAA GGGAGATATG TTGTGAAAGA 
AGCAGTAGCA GTGAGTCAAC AGGGACGCCA TCAAATCCGG ATCTTGATGC TGGTGTAAGT 
GAACATTCAG GTGATTGGTT GGATCAGGAT TCAGTTTCAG ATCAATTCAG TGTAGAATTT 
GAAGTTGAGT CTCTTGATTC AGAAGATTAT AGCCTTAGTG AAGAAGGACA AGAACTCTCA 
GATGAAGATG ATGAGGTGTA TCGAGTAACT GTGTATCAGG CAGGGGAGAG TGATACAGAT 
TCATTTGAAG AAGATCCTGA AATTTCCTTA GCTGACTATT GGAAGTGTAC CTCATGCAAT 
GAAATGAATC CTCCTCTTCC ACCTCATTGC AATAGATGTT GGGCCCTTCG TGAGAATTGG 
CTTCCTGAAG ATAAAGGGAA AATCCCTGAG AAAGCCACAC CAGAAAACTC AACACAAGTA 
GAAGAGGGCT TTGATGTCCC TGATTGTAAA AAAGCTGCAG CCAGTGATTC CAGAGAATCA 
TGTGCTGAGG AAATTGATGA TAAAATCACA CAAGCCTCCC ACTCCCAAGA AAGTGAGGAC 
TATTCTCAGC CATCAACTTC TAACAGCATC ATTTATAGCA GCCAAGAAGA TGTCAAAGAA 
TTTGAGAGAG AAGAAACACA AGACAAAGAA GAAATTGTGG AATCTAGTTT TCCCCTTAAT 
GCCATTGAAC CTTGTGTGAT TTGCCAAGGT CGACCTAAAA ACGGTTGCAT TGTTCATGGC 
AAAACAGGAC ATCTTATGGC ATGCTTCACG TGTGCAAAGA AGCTAAAGAA AAGGAATAAG 
CCCTGTCCAG TATGTAGACA ACCAATTCAA ATGATTGTGC TAACTTATTT CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625508.1
CDS	180..1496
Translation	MKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_005625451.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625451.3

ORF Insert Sequence:

ATGAAAGAGG TGATATTTTA TCTTGGCCAG TATATTATGA CTAAACGATT GTATGATGAG 
AAGCAACAGC ATATTGTGTA TTGTTCCAAT GATCTTCTAG GAGATTTGTT TGGAGTGCCA 
AGCTTCTCTG TGAAAGAGCA CAGGAAAATA TATACAATGA TCTACAGAAA CTTGGTAGTA 
GTCAATCAGC ATGAACCATC AGATTCAGGC ACATCTGTGA GTGAAAATAG CTGTCACCGT 
GAAGGTGGGA GTGATCAAAA GGACCCTGTG CAAGAGTTGC AGGAAGAGAA ACCTTCATCT 
TCAGATTTGA TTTCTAGACC ATCTACCTCA TCTAGAAGGA GAACAATTAG TGAGACAGAA 
GAACATGCAG ATGATTTACC TGGTGAACGG CAGAGAAAGC GCCACAAATC TGATAGTATT 
TCCCTTTCCT TTGATGAAAG CCTTGCTCTG TGTGTAATAA GGGAGATATG TTGTGAAAGA 
AGCAGTAGCA GTGAGTCAAC AGGGACGCCA TCAAATCCGG ATCTTGATGC TGGTGTAAGT 
GAACATTCAG GTGATTGGTT GGATCAGGAT TCAGTTTCAG ATCAATTCAG TGTAGAATTT 
GAAGTTGAGT CTCTTGATTC AGAAGATTAT AGCCTTAGTG AAGAAGGACA AGAACTCTCA 
GATGAAGATG ATGAGGTGTA TCGAGTAACT GTGTATCAGG CAGGGGAGAG TGATACAGAT 
TCATTTGAAG AAGATCCTGA AATTTCCTTA GCTGACTATT GGAAGTGTAC CTCATGCAAT 
GAAATGAATC CTCCTCTTCC ACCTCATTGC AATAGATGTT GGGCCCTTCG TGAGAATTGG 
CTTCCTGAAG ATAAAGGGAA AATCCCTGAG AAAGCCACAC CAGAAAACTC AACACAAGTA 
GAAGAGGGCT TTGATGTCCC TGATTGTAAA AAAGCTGCAG CCAGTGATTC CAGAGAATCA 
TGTGCTGAGG AAATTGATGA TAAAATCACA CAAGCCTCCC ACTCCCAAGA AAGTGAGGAC 
TATTCTCAGC CATCAACTTC TAACAGCATC ATTTATAGCA GCCAAGAAGA TGTCAAAGAA 
TTTGAGAGAG AAGAAACACA AGACAAAGAA GAAATTGTGG AATCTAGTTT TCCCCTTAAT 
GCCATTGAAC CTTGTGTGAT TTGCCAAGGT CGACCTAAAA ACGGTTGCAT TGTTCATGGC 
AAAACAGGAC ATCTTATGGC ATGCTTCACG TGTGCAAAGA AGCTAAAGAA AAGGAATAAG 
CCCTGTCCAG TATGTAGACA ACCAATTCAA ATGATTGTGC TAACTTATTT CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_022423982.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279690.1 Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe12971
Clone ID Related Accession (Same CDS sequence)	XM_005625451.2 , XM_005625451.3 , XM_022423982.1
Accession Version	XM_022423982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGG TGATATTTTA TCTTGGCCAG TATATTATGA CTAAACGATT GTATGATGAG 
AAGCAACAGC ATATTGTGTA TTGTTCCAAT GATCTTCTAG GAGATTTGTT TGGAGTGCCA 
AGCTTCTCTG TGAAAGAGCA CAGGAAAATA TATACAATGA TCTACAGAAA CTTGGTAGTA 
GTCAATCAGC ATGAACCATC AGATTCAGGC ACATCTGTGA GTGAAAATAG CTGTCACCGT 
GAAGGTGGGA GTGATCAAAA GGACCCTGTG CAAGAGTTGC AGGAAGAGAA ACCTTCATCT 
TCAGATTTGA TTTCTAGACC ATCTACCTCA TCTAGAAGGA GAACAATTAG TGAGACAGAA 
GAACATGCAG ATGATTTACC TGGTGAACGG CAGAGAAAGC GCCACAAATC TGATAGTATT 
TCCCTTTCCT TTGATGAAAG CCTTGCTCTG TGTGTAATAA GGGAGATATG TTGTGAAAGA 
AGCAGTAGCA GTGAGTCAAC AGGGACGCCA TCAAATCCGG ATCTTGATGC TGGTGTAAGT 
GAACATTCAG GTGATTGGTT GGATCAGGAT TCAGTTTCAG ATCAATTCAG TGTAGAATTT 
GAAGTTGAGT CTCTTGATTC AGAAGATTAT AGCCTTAGTG AAGAAGGACA AGAACTCTCA 
GATGAAGATG ATGAGGTGTA TCGAGTAACT GTGTATCAGG CAGGGGAGAG TGATACAGAT 
TCATTTGAAG AAGATCCTGA AATTTCCTTA GCTGACTATT GGAAGTGTAC CTCATGCAAT 
GAAATGAATC CTCCTCTTCC ACCTCATTGC AATAGATGTT GGGCCCTTCG TGAGAATTGG 
CTTCCTGAAG ATAAAGGGAA AATCCCTGAG AAAGCCACAC CAGAAAACTC AACACAAGTA 
GAAGAGGGCT TTGATGTCCC TGATTGTAAA AAAGCTGCAG CCAGTGATTC CAGAGAATCA 
TGTGCTGAGG AAATTGATGA TAAAATCACA CAAGCCTCCC ACTCCCAAGA AAGTGAGGAC 
TATTCTCAGC CATCAACTTC TAACAGCATC ATTTATAGCA GCCAAGAAGA TGTCAAAGAA 
TTTGAGAGAG AAGAAACACA AGACAAAGAA GAAATTGTGG AATCTAGTTT TCCCCTTAAT 
GCCATTGAAC CTTGTGTGAT TTGCCAAGGT CGACCTAAAA ACGGTTGCAT TGTTCATGGC 
AAAACAGGAC ATCTTATGGC ATGCTTCACG TGTGCAAAGA AGCTAAAGAA AAGGAATAAG 
CCCTGTCCAG TATGTAGACA ACCAATTCAA ATGATTGTGC TAACTTATTT CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279690.1
CDS	154..1470
Translation	MKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_022423982.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423982.1

ORF Insert Sequence:

ATGAAAGAGG TGATATTTTA TCTTGGCCAG TATATTATGA CTAAACGATT GTATGATGAG 
AAGCAACAGC ATATTGTGTA TTGTTCCAAT GATCTTCTAG GAGATTTGTT TGGAGTGCCA 
AGCTTCTCTG TGAAAGAGCA CAGGAAAATA TATACAATGA TCTACAGAAA CTTGGTAGTA 
GTCAATCAGC ATGAACCATC AGATTCAGGC ACATCTGTGA GTGAAAATAG CTGTCACCGT 
GAAGGTGGGA GTGATCAAAA GGACCCTGTG CAAGAGTTGC AGGAAGAGAA ACCTTCATCT 
TCAGATTTGA TTTCTAGACC ATCTACCTCA TCTAGAAGGA GAACAATTAG TGAGACAGAA 
GAACATGCAG ATGATTTACC TGGTGAACGG CAGAGAAAGC GCCACAAATC TGATAGTATT 
TCCCTTTCCT TTGATGAAAG CCTTGCTCTG TGTGTAATAA GGGAGATATG TTGTGAAAGA 
AGCAGTAGCA GTGAGTCAAC AGGGACGCCA TCAAATCCGG ATCTTGATGC TGGTGTAAGT 
GAACATTCAG GTGATTGGTT GGATCAGGAT TCAGTTTCAG ATCAATTCAG TGTAGAATTT 
GAAGTTGAGT CTCTTGATTC AGAAGATTAT AGCCTTAGTG AAGAAGGACA AGAACTCTCA 
GATGAAGATG ATGAGGTGTA TCGAGTAACT GTGTATCAGG CAGGGGAGAG TGATACAGAT 
TCATTTGAAG AAGATCCTGA AATTTCCTTA GCTGACTATT GGAAGTGTAC CTCATGCAAT 
GAAATGAATC CTCCTCTTCC ACCTCATTGC AATAGATGTT GGGCCCTTCG TGAGAATTGG 
CTTCCTGAAG ATAAAGGGAA AATCCCTGAG AAAGCCACAC CAGAAAACTC AACACAAGTA 
GAAGAGGGCT TTGATGTCCC TGATTGTAAA AAAGCTGCAG CCAGTGATTC CAGAGAATCA 
TGTGCTGAGG AAATTGATGA TAAAATCACA CAAGCCTCCC ACTCCCAAGA AAGTGAGGAC 
TATTCTCAGC CATCAACTTC TAACAGCATC ATTTATAGCA GCCAAGAAGA TGTCAAAGAA 
TTTGAGAGAG AAGAAACACA AGACAAAGAA GAAATTGTGG AATCTAGTTT TCCCCTTAAT 
GCCATTGAAC CTTGTGTGAT TTGCCAAGGT CGACCTAAAA ACGGTTGCAT TGTTCATGGC 
AAAACAGGAC ATCTTATGGC ATGCTTCACG TGTGCAAAGA AGCTAAAGAA AAGGAATAAG 
CCCTGTCCAG TATGTAGACA ACCAATTCAA ATGATTGTGC TAACTTATTT CCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_022423983.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279691.1 Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X4, mRNA.

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CloneID	OCe291198
Clone ID Related Accession (Same CDS sequence)	XM_022423983.1
Accession Version	XM_022423983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AAGAACATGC AGATGATTTA 
CCTGGTGAAC GGCAGAGAAA GCGCCACAAA TCTGATAGTA TTTCCCTTTC CTTTGATGAA 
AGCCTTGCTC TGTGTGTAAT AAGGGAGATA TGTTGTGAAA GAAGCAGTAG CAGTGAGTCA 
ACAGGGACGC CATCAAATCC GGATCTTGAT GCTGGTGTAA GTGAACATTC AGGTGATTGG 
TTGGATCAGG ATTCAGTTTC AGATCAATTC AGTGTAGAAT TTGAAGTTGA GTCTCTTGAT 
TCAGAAGATT ATAGCCTTAG TGAAGAAGGA CAAGAACTCT CAGATGAAGA TGATGAGGTG 
TATCGAGTAA CTGTGTATCA GGCAGGGGAG AGTGATACAG ATTCATTTGA AGAAGATCCT 
GAAATTTCCT TAGCTGACTA TTGGAAGTGT ACCTCATGCA ATGAAATGAA TCCTCCTCTT 
CCACCTCATT GCAATAGATG TTGGGCCCTT CGTGAGAATT GGCTTCCTGA AGATAAAGGG 
AAAATCCCTG AGAAAGCCAC ACCAGAAAAC TCAACACAAG TAGAAGAGGG CTTTGATGTC 
CCTGATTGTA AAAAAGCTGC AGCCAGTGAT TCCAGAGAAT CATGTGCTGA GGAAATTGAT 
GATAAAATCA CACAAGCCTC CCACTCCCAA GAAAGTGAGG ACTATTCTCA GCCATCAACT 
TCTAACAGCA TCATTTATAG CAGCCAAGAA GATGTCAAAG AATTTGAGAG AGAAGAAACA 
CAAGACAAAG AAGAAATTGT GGAATCTAGT TTTCCCCTTA ATGCCATTGA ACCTTGTGTG 
ATTTGCCAAG GTCGACCTAA AAACGGTTGC ATTGTTCATG GCAAAACAGG ACATCTTATG 
GCATGCTTCA CGTGTGCAAA GAAGCTAAAG AAAAGGAATA AGCCCTGTCC AGTATGTAGA 
CAACCAATTC AAATGATTGT GCTAACTTAT TTCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279691.1
CDS	151..1449
Translation	MCNTNMSVSTDGAVSTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_022423983.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423983.1

ORF Insert Sequence:

ATGTGCAATA CCAACATGTC TGTGTCTACT GATGGTGCTG TAAGCACCTC ACAGATTCCA 
GCTTCGGAGC AGGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT GTTGAAGTCT 
GTAGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTGA TATTTTATCT TGGCCAGTAT 
ATTATGACTA AACGATTGTA TGATGAGAAG CAACAGCATA TTGTGTATTG TTCCAATGAT 
CTTCTAGGAG ATTTGTTTGG AGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 
ACAATGATCT ACAGAAACTT GGTAGTAGTC AATCAGCATG AAGAACATGC AGATGATTTA 
CCTGGTGAAC GGCAGAGAAA GCGCCACAAA TCTGATAGTA TTTCCCTTTC CTTTGATGAA 
AGCCTTGCTC TGTGTGTAAT AAGGGAGATA TGTTGTGAAA GAAGCAGTAG CAGTGAGTCA 
ACAGGGACGC CATCAAATCC GGATCTTGAT GCTGGTGTAA GTGAACATTC AGGTGATTGG 
TTGGATCAGG ATTCAGTTTC AGATCAATTC AGTGTAGAAT TTGAAGTTGA GTCTCTTGAT 
TCAGAAGATT ATAGCCTTAG TGAAGAAGGA CAAGAACTCT CAGATGAAGA TGATGAGGTG 
TATCGAGTAA CTGTGTATCA GGCAGGGGAG AGTGATACAG ATTCATTTGA AGAAGATCCT 
GAAATTTCCT TAGCTGACTA TTGGAAGTGT ACCTCATGCA ATGAAATGAA TCCTCCTCTT 
CCACCTCATT GCAATAGATG TTGGGCCCTT CGTGAGAATT GGCTTCCTGA AGATAAAGGG 
AAAATCCCTG AGAAAGCCAC ACCAGAAAAC TCAACACAAG TAGAAGAGGG CTTTGATGTC 
CCTGATTGTA AAAAAGCTGC AGCCAGTGAT TCCAGAGAAT CATGTGCTGA GGAAATTGAT 
GATAAAATCA CACAAGCCTC CCACTCCCAA GAAAGTGAGG ACTATTCTCA GCCATCAACT 
TCTAACAGCA TCATTTATAG CAGCCAAGAA GATGTCAAAG AATTTGAGAG AGAAGAAACA 
CAAGACAAAG AAGAAATTGT GGAATCTAGT TTTCCCCTTA ATGCCATTGA ACCTTGTGTG 
ATTTGCCAAG GTCGACCTAA AAACGGTTGC ATTGTTCATG GCAAAACAGG ACATCTTATG 
GCATGCTTCA CGTGTGCAAA GAAGCTAAAG AAAAGGAATA AGCCCTGTCC AGTATGTAGA 
CAACCAATTC AAATGATTGT GCTAACTTAT TTCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDM2 ( XM_022423984.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279692.1 Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X5, mRNA.

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CloneID	OCe291199
Clone ID Related Accession (Same CDS sequence)	XM_022423984.1
Accession Version	XM_022423984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Mdm2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAC GATTGTATGA TGAGAAGCAA CAGCATATTG TGTATTGTTC CAATGATCTT 
CTAGGAGATT TGTTTGGAGT GCCAAGCTTC TCTGTGAAAG AGCACAGGAA AATATATACA 
ATGATCTACA GAAACTTGGT AGTAGTCAAT CAGCATGAAC CATCAGATTC AGGCACATCT 
GTGAGTGAAA ATAGCTGTCA CCGTGAAGGT GGGAGTGATC AAAAGGACCC TGTGCAAGAG 
TTGCAGGAAG AGAAACCTTC ATCTTCAGAT TTGATTTCTA GACCATCTAC CTCATCTAGA 
AGGAGAACAA TTAGTGAGAC AGAAGAACAT GCAGATGATT TACCTGGTGA ACGGCAGAGA 
AAGCGCCACA AATCTGATAG TATTTCCCTT TCCTTTGATG AAAGCCTTGC TCTGTGTGTA 
ATAAGGGAGA TATGTTGTGA AAGAAGCAGT AGCAGTGAGT CAACAGGGAC GCCATCAAAT 
CCGGATCTTG ATGCTGGTGT AAGTGAACAT TCAGGTGATT GGTTGGATCA GGATTCAGTT 
TCAGATCAAT TCAGTGTAGA ATTTGAAGTT GAGTCTCTTG ATTCAGAAGA TTATAGCCTT 
AGTGAAGAAG GACAAGAACT CTCAGATGAA GATGATGAGG TGTATCGAGT AACTGTGTAT 
CAGGCAGGGG AGAGTGATAC AGATTCATTT GAAGAAGATC CTGAAATTTC CTTAGCTGAC 
TATTGGAAGT GTACCTCATG CAATGAAATG AATCCTCCTC TTCCACCTCA TTGCAATAGA 
TGTTGGGCCC TTCGTGAGAA TTGGCTTCCT GAAGATAAAG GGAAAATCCC TGAGAAAGCC 
ACACCAGAAA ACTCAACACA AGTAGAAGAG GGCTTTGATG TCCCTGATTG TAAAAAAGCT 
GCAGCCAGTG ATTCCAGAGA ATCATGTGCT GAGGAAATTG ATGATAAAAT CACACAAGCC 
TCCCACTCCC AAGAAAGTGA GGACTATTCT CAGCCATCAA CTTCTAACAG CATCATTTAT 
AGCAGCCAAG AAGATGTCAA AGAATTTGAG AGAGAAGAAA CACAAGACAA AGAAGAAATT 
GTGGAATCTA GTTTTCCCCT TAATGCCATT GAACCTTGTG TGATTTGCCA AGGTCGACCT 
AAAAACGGTT GCATTGTTCA TGGCAAAACA GGACATCTTA TGGCATGCTT CACGTGTGCA 
AAGAAGCTAA AGAAAAGGAA TAAGCCCTGT CCAGTATGTA GACAACCAAT TCAAATGATT 
GTGCTAACTT ATTTCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279692.1
CDS	272..1552
Translation	MTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP

Target ORF information:

RefSeq Version	XM_022423984.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MDM2 proto-oncogene (MDM2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423984.1

ORF Insert Sequence:

ATGACTAAAC GATTGTATGA TGAGAAGCAA CAGCATATTG TGTATTGTTC CAATGATCTT 
CTAGGAGATT TGTTTGGAGT GCCAAGCTTC TCTGTGAAAG AGCACAGGAA AATATATACA 
ATGATCTACA GAAACTTGGT AGTAGTCAAT CAGCATGAAC CATCAGATTC AGGCACATCT 
GTGAGTGAAA ATAGCTGTCA CCGTGAAGGT GGGAGTGATC AAAAGGACCC TGTGCAAGAG 
TTGCAGGAAG AGAAACCTTC ATCTTCAGAT TTGATTTCTA GACCATCTAC CTCATCTAGA 
AGGAGAACAA TTAGTGAGAC AGAAGAACAT GCAGATGATT TACCTGGTGA ACGGCAGAGA 
AAGCGCCACA AATCTGATAG TATTTCCCTT TCCTTTGATG AAAGCCTTGC TCTGTGTGTA 
ATAAGGGAGA TATGTTGTGA AAGAAGCAGT AGCAGTGAGT CAACAGGGAC GCCATCAAAT 
CCGGATCTTG ATGCTGGTGT AAGTGAACAT TCAGGTGATT GGTTGGATCA GGATTCAGTT 
TCAGATCAAT TCAGTGTAGA ATTTGAAGTT GAGTCTCTTG ATTCAGAAGA TTATAGCCTT 
AGTGAAGAAG GACAAGAACT CTCAGATGAA GATGATGAGG TGTATCGAGT AACTGTGTAT 
CAGGCAGGGG AGAGTGATAC AGATTCATTT GAAGAAGATC CTGAAATTTC CTTAGCTGAC 
TATTGGAAGT GTACCTCATG CAATGAAATG AATCCTCCTC TTCCACCTCA TTGCAATAGA 
TGTTGGGCCC TTCGTGAGAA TTGGCTTCCT GAAGATAAAG GGAAAATCCC TGAGAAAGCC 
ACACCAGAAA ACTCAACACA AGTAGAAGAG GGCTTTGATG TCCCTGATTG TAAAAAAGCT 
GCAGCCAGTG ATTCCAGAGA ATCATGTGCT GAGGAAATTG ATGATAAAAT CACACAAGCC 
TCCCACTCCC AAGAAAGTGA GGACTATTCT CAGCCATCAA CTTCTAACAG CATCATTTAT 
AGCAGCCAAG AAGATGTCAA AGAATTTGAG AGAGAAGAAA CACAAGACAA AGAAGAAATT 
GTGGAATCTA GTTTTCCCCT TAATGCCATT GAACCTTGTG TGATTTGCCA AGGTCGACCT 
AAAAACGGTT GCATTGTTCA TGGCAAAACA GGACATCTTA TGGCATGCTT CACGTGTGCA 
AAGAAGCTAA AGAAAAGGAA TAAGCCCTGT CCAGTATGTA GACAACCAAT TCAAATGATT 
GTGCTAACTT ATTTCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphism of P53-Ets/AP1 transactivation region of MDM2 oncogene and its immunohistochemical analysis in canine tumours.
Veterinary and comparative oncology14(2)137-46(2016 Jun)
Rezaie A,Tabandeh MR,Noori SM

Cloning, sequence analysis and expression of the cDNAs encoding the canine and equine homologues of the mouse double minute 2 (mdm2) proto-oncogene.
Cancer letters152(1)9-13(2000 Apr)
Nasir L,Burr PD,McFarlane ST,Gault E,Thompson H,Argyle DJ

A novel exon within the mdm2 gene modulates translation initiation in vitro and disrupts the p53-binding domain of mdm2 protein.
Oncogene18(50)7026-33(1999 Nov)
Veldhoen N,Metcalfe S,Milner J

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MDM2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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