ESR1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESR1
Entrez Gene ID	403640
Full Name	estrogen receptor 1
Synonyms	ERALPHA
General protein information	Preferred Names estrogen receptor 1 Names estrogen receptor
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014113908.2	XP_013969383.1	estrogen receptor isoform X1
XM_014113908.1	XP_013969383.1	estrogen receptor isoform X2
XM_014114137.1	XP_013969612.1	estrogen receptor isoform X1
NM_001286958.1	NP_001273887.1	estrogen receptor
XM_005615522.2	XP_005615579.1	estrogen receptor isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	esr1	NP_694491.1
Pan troglodytes (chimpanzee)	ESR1	XP_003311598.1
Canis lupus familiaris (dog)	ESR1	XP_533454.2
Gallus gallus (chicken)	ESR1	NP_990514.1
Rattus norvegicus (Norway rat)	Esr1	NP_036821.1
Xenopus tropicalis (tropical clawed frog)	esr1	NP_988866.1
	ESR1	XP_001097228.1
Bos taurus (cattle)	ESR1	NP_001001443.1
Mus musculus (house mouse)	Esr1	NP_031982.1
Homo sapiens (human)	ESR1	NP_001116212.1

Gene Ontology
Bibliography

Related articles in PubMed

Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs.
Chuffa LG, Lupi J?nior LA, da Maia Lima AF
Tissue & cell48(1)10-7(2016 Feb)

The ESR1 gene is associated with risk for canine mammary tumours.
Borge KS, Melin M, Rivera P, Thoresen SI, Webster MT, von Euler H, Lindblad-Toh K, Lingaas F
BMC veterinary research969(2013 Apr)

Homozygosity of single nucleotide polymorphisms in the 3' region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas.
Pathirana IN, Tanaka K, Kawate N, Tsuji M, Hatoya S, Inaba T, Tamada H
Animal science journal = Nihon chikusan Gakkaiho82(3)390-5(2011 Jun)

Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas.
Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H
The Journal of reproduction and development56(4)405-10(2010 Aug)

ERalpha increases expression and interacts with TERT in cataractous canine lens epithelial cells.
Colitz CM, Sugimoto Y, Lu P, Barden CA, Thomas-Ahner J, Chandler HL
Molecular vision152259-67(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We concluded that sex steroid receptors and apoptotic proteins are differentially expressed in the follicles of adult dogs with polystic ovaries
Title: Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs.

associated with risk for mammary tumours
Title: The ESR1 gene is associated with risk for canine mammary tumours.

These results imply that homozygosity for the allele, genotype and haplotype distribution within the block at the 3' end of ESR1 is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas.
Title: Homozygosity of single nucleotide polymorphisms in the 3' region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas.

Analysis of SNPs in 3'region of esr1 in cryptorchid dogs.
Title: Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas.

TERT interacted with ERalpha via the estrogen response element (ERE) in cataractous lens epithelial cells (LEC) but not in normal LEC.
Title: ERalpha increases expression and interacts with TERT in cataractous canine lens epithelial cells.

The following ESR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00567	NM_001286958.1 Latest version!	Canis lupus familiaris estrogen receptor 1 (ESR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe00567	XM_005615522.2 Latest version!	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe00567	XM_014114137.1 Latest version!	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe34344	XM_014113908.2 Latest version!	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
OCe34344	XM_014113908.1	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESR1 ( NM_001286958.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001273887.1 Canis lupus familiaris estrogen receptor 1 (ESR1), mRNA.

Price & Availability↑

CloneID	OCe00567
Clone ID Related Accession (Same CDS sequence)	XM_014114137.1 , NM_001286958.1 , XM_005615522.2
Accession Version	NM_001286958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Canis lupus familiaris(dog)
Product	estrogen receptor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03000275.1, AAEX03000276.1 and AAEX03000277.1. On Nov 21, 2013 this sequence version replaced XM_533454.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMN00013597 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGGAGTGTAC 
ACATTTCTGT CCAGCACCCT GAAGTCTCTG GAAGAGAAGG ACCATATCCA CCGTATCTTA 
GACAAGATCA CAGACACCTT GATCCATCTG ATGGCCAAAG CAGGCCTCAC TCTGCAGCAG 
CAGCACCGGC GTCTGGCCCA GCTCCTCCTC ATCCTCTCCC ACATCAGGCA CATGAGTAAC 
AAAGGCATGG AGCATCTGTA CAACATGAAG TGCAAGAATG TGGTGCCTCT CTACGACCTG 
CTGCTGGAGA TGCTGGATGC CCACCGCCTG CACGCCCCAG CCAGCCGTGG GGGTGTGCCC 
ATGGAGGAGA CAAACCAGAG CCAGCTGGCC ACCACGGGCC CAACTTCATC ACATTCCTTG 
CAAACGTATT ATATCACCGA GGAGGCAGGG AATTTCCCTA CCACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273887.1
CDS	192..1982
Translation	MTLHTKASGMALLHQIQGPELDSLNRPQLKIPLERPLGEVYVDSSKPAVYNYPEAGAYDFNAAPAAPAPLYGQSGLGYGPGSEAVAAAAFGANGLGGFPPLNSMSPSPPVLLHPPPQLSSFLHPHGQQVPYYLENEPSGYAVRQAGPPAFYRPNSDNRRQGGRERLASTSDKGNMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEVGSSGDVRTSSLWPSPLLIKHTKKNSPALSLTADQMVSALLEAEPPIIYSDYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLFFAPNLLLDRNQGKCVEGIVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRILDKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPASRGGVPMEETNQSQLATTGPTSSHSLQTYYITEEAGNFPTTV

Target ORF information:

RefSeq Version	NM_001286958.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris estrogen receptor 1 (ESR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286958.1

ORF Insert Sequence:

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGGAGTGTAC 
ACATTTCTGT CCAGCACCCT GAAGTCTCTG GAAGAGAAGG ACCATATCCA CCGTATCTTA 
GACAAGATCA CAGACACCTT GATCCATCTG ATGGCCAAAG CAGGCCTCAC TCTGCAGCAG 
CAGCACCGGC GTCTGGCCCA GCTCCTCCTC ATCCTCTCCC ACATCAGGCA CATGAGTAAC 
AAAGGCATGG AGCATCTGTA CAACATGAAG TGCAAGAATG TGGTGCCTCT CTACGACCTG 
CTGCTGGAGA TGCTGGATGC CCACCGCCTG CACGCCCCAG CCAGCCGTGG GGGTGTGCCC 
ATGGAGGAGA CAAACCAGAG CCAGCTGGCC ACCACGGGCC CAACTTCATC ACATTCCTTG 
CAAACGTATT ATATCACCGA GGAGGCAGGG AATTTCCCTA CCACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESR1 ( XM_014114137.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969612.1 Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe00567
Clone ID Related Accession (Same CDS sequence)	XM_014114137.1 , NM_001286958.1 , XM_005615522.2
Accession Version	XM_014114137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	estrogen receptor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGGAGTGTAC 
ACATTTCTGT CCAGCACCCT GAAGTCTCTG GAAGAGAAGG ACCATATCCA CCGTATCTTA 
GACAAGATCA CAGACACCTT GATCCATCTG ATGGCCAAAG CAGGCCTCAC TCTGCAGCAG 
CAGCACCGGC GTCTGGCCCA GCTCCTCCTC ATCCTCTCCC ACATCAGGCA CATGAGTAAC 
AAAGGCATGG AGCATCTGTA CAACATGAAG TGCAAGAATG TGGTGCCTCT CTACGACCTG 
CTGCTGGAGA TGCTGGATGC CCACCGCCTG CACGCCCCAG CCAGCCGTGG GGGTGTGCCC 
ATGGAGGAGA CAAACCAGAG CCAGCTGGCC ACCACGGGCC CAACTTCATC ACATTCCTTG 
CAAACGTATT ATATCACCGA GGAGGCAGGG AATTTCCCTA CCACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969612.1
CDS	128..1918
Translation	MTLHTKASGMALLHQIQGPELDSLNRPQLKIPLERPLGEVYVDSSKPAVYNYPEAGAYDFNAAPAAPAPLYGQSGLGYGPGSEAVAAAAFGANGLGGFPPLNSMSPSPPVLLHPPPQLSSFLHPHGQQVPYYLENEPSGYAVRQAGPPAFYRPNSDNRRQGGRERLASTSDKGNMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEVGSSGDVRTSSLWPSPLLIKHTKKNSPALSLTADQMVSALLEAEPPIIYSDYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLFFAPNLLLDRNQGKCVEGIVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRILDKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPASRGGVPMEETNQSQLATTGPTSSHSLQTYYITEEAGNFPTTV

Target ORF information:

RefSeq Version	XM_014114137.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114137.1

ORF Insert Sequence:

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGGAGTGTAC 
ACATTTCTGT CCAGCACCCT GAAGTCTCTG GAAGAGAAGG ACCATATCCA CCGTATCTTA 
GACAAGATCA CAGACACCTT GATCCATCTG ATGGCCAAAG CAGGCCTCAC TCTGCAGCAG 
CAGCACCGGC GTCTGGCCCA GCTCCTCCTC ATCCTCTCCC ACATCAGGCA CATGAGTAAC 
AAAGGCATGG AGCATCTGTA CAACATGAAG TGCAAGAATG TGGTGCCTCT CTACGACCTG 
CTGCTGGAGA TGCTGGATGC CCACCGCCTG CACGCCCCAG CCAGCCGTGG GGGTGTGCCC 
ATGGAGGAGA CAAACCAGAG CCAGCTGGCC ACCACGGGCC CAACTTCATC ACATTCCTTG 
CAAACGTATT ATATCACCGA GGAGGCAGGG AATTTCCCTA CCACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESR1 ( XM_005615522.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005615579.1 Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe00567
Clone ID Related Accession (Same CDS sequence)	XM_014114137.1 , NM_001286958.1 , XM_005615522.2
Accession Version	XM_005615522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	estrogen receptor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGGAGTGTAC 
ACATTTCTGT CCAGCACCCT GAAGTCTCTG GAAGAGAAGG ACCATATCCA CCGTATCTTA 
GACAAGATCA CAGACACCTT GATCCATCTG ATGGCCAAAG CAGGCCTCAC TCTGCAGCAG 
CAGCACCGGC GTCTGGCCCA GCTCCTCCTC ATCCTCTCCC ACATCAGGCA CATGAGTAAC 
AAAGGCATGG AGCATCTGTA CAACATGAAG TGCAAGAATG TGGTGCCTCT CTACGACCTG 
CTGCTGGAGA TGCTGGATGC CCACCGCCTG CACGCCCCAG CCAGCCGTGG GGGTGTGCCC 
ATGGAGGAGA CAAACCAGAG CCAGCTGGCC ACCACGGGCC CAACTTCATC ACATTCCTTG 
CAAACGTATT ATATCACCGA GGAGGCAGGG AATTTCCCTA CCACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615579.1
CDS	155..1945
Translation	MTLHTKASGMALLHQIQGPELDSLNRPQLKIPLERPLGEVYVDSSKPAVYNYPEAGAYDFNAAPAAPAPLYGQSGLGYGPGSEAVAAAAFGANGLGGFPPLNSMSPSPPVLLHPPPQLSSFLHPHGQQVPYYLENEPSGYAVRQAGPPAFYRPNSDNRRQGGRERLASTSDKGNMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEVGSSGDVRTSSLWPSPLLIKHTKKNSPALSLTADQMVSALLEAEPPIIYSDYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLFFAPNLLLDRNQGKCVEGIVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRILDKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPASRGGVPMEETNQSQLATTGPTSSHSLQTYYITEEAGNFPTTV

Target ORF information:

RefSeq Version	XM_005615522.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615522.2

ORF Insert Sequence:

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGGAGTGTAC 
ACATTTCTGT CCAGCACCCT GAAGTCTCTG GAAGAGAAGG ACCATATCCA CCGTATCTTA 
GACAAGATCA CAGACACCTT GATCCATCTG ATGGCCAAAG CAGGCCTCAC TCTGCAGCAG 
CAGCACCGGC GTCTGGCCCA GCTCCTCCTC ATCCTCTCCC ACATCAGGCA CATGAGTAAC 
AAAGGCATGG AGCATCTGTA CAACATGAAG TGCAAGAATG TGGTGCCTCT CTACGACCTG 
CTGCTGGAGA TGCTGGATGC CCACCGCCTG CACGCCCCAG CCAGCCGTGG GGGTGTGCCC 
ATGGAGGAGA CAAACCAGAG CCAGCTGGCC ACCACGGGCC CAACTTCATC ACATTCCTTG 
CAAACGTATT ATATCACCGA GGAGGCAGGG AATTTCCCTA CCACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESR1 ( XM_014113908.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969383.1 Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X1, mRNA.

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CloneID	OCe34344
Clone ID Related Accession (Same CDS sequence)	XM_014113908.2 , XM_014113908.1
Accession Version	XM_014113908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	estrogen receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGTCATTTGT 
CCGGTACGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969383.1
CDS	209..1600
Translation	MTLHTKASGMALLHQIQGPELDSLNRPQLKIPLERPLGEVYVDSSKPAVYNYPEAGAYDFNAAPAAPAPLYGQSGLGYGPGSEAVAAAAFGANGLGGFPPLNSMSPSPPVLLHPPPQLSSFLHPHGQQVPYYLENEPSGYAVRQAGPPAFYRPNSDNRRQGGRERLASTSDKGNMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEVGSSGDVRTSSLWPSPLLIKHTKKNSPALSLTADQMVSALLEAEPPIIYSDYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLFFAPNLLLDRNQGKCVEGIVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSVICPVR

Target ORF information:

RefSeq Version	XM_014113908.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113908.2

ORF Insert Sequence:

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGTCATTTGT 
CCGGTACGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESR1 ( XM_014113908.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969383.1 Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe34344
Clone ID Related Accession (Same CDS sequence)	XM_014113908.2 , XM_014113908.1
Accession Version	XM_014113908.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	estrogen receptor isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGTCATTTGT 
CCGGTACGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969383.1
CDS	214..1605
Translation	MTLHTKASGMALLHQIQGPELDSLNRPQLKIPLERPLGEVYVDSSKPAVYNYPEAGAYDFNAAPAAPAPLYGQSGLGYGPGSEAVAAAAFGANGLGGFPPLNSMSPSPPVLLHPPPQLSSFLHPHGQQVPYYLENEPSGYAVRQAGPPAFYRPNSDNRRQGGRERLASTSDKGNMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEVGSSGDVRTSSLWPSPLLIKHTKKNSPALSLTADQMVSALLEAEPPIIYSDYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLFFAPNLLLDRNQGKCVEGIVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSVICPVR

Target ORF information:

RefSeq Version	XM_014113908.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris estrogen receptor 1 (ESR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113908.1

ORF Insert Sequence:

ATGACCCTCC ACACCAAGGC GTCCGGAATG GCCCTGCTGC ACCAGATCCA AGGGCCCGAG 
CTGGACTCCC TGAACCGCCC GCAGCTCAAG ATCCCCCTGG AGCGGCCCCT GGGCGAGGTG 
TACGTGGACA GCAGCAAGCC CGCCGTGTAC AACTACCCCG AGGCGGGCGC CTACGACTTC 
AACGCCGCGC CCGCCGCGCC CGCGCCCCTC TACGGCCAGT CGGGCCTCGG CTACGGCCCC 
GGGTCCGAGG CGGTGGCGGC CGCGGCGTTC GGCGCCAACG GCCTGGGGGG CTTCCCGCCG 
CTCAACAGCA TGTCCCCGAG CCCGCCGGTG CTGCTGCACC CGCCGCCTCA GCTCTCGTCC 
TTCCTGCACC CCCACGGCCA GCAGGTGCCC TATTACCTGG AGAACGAGCC GAGCGGCTAC 
GCGGTGCGCC AGGCCGGCCC GCCCGCCTTC TACAGGCCAA ATTCAGATAA TCGACGCCAG 
GGTGGCAGAG AGAGATTGGC CAGTACCAGT GACAAAGGAA ACATGGCCAT GGAGTCTGCC 
AAGGAGACTC GCTACTGTGC AGTGTGCAAT GACTATGCCT CAGGCTACCA TTATGGAGTC 
TGGTCCTGTG AGGGCTGTAA GGCCTTTTTC AAGAGAAGTA TTCAAGGACA TAATGACTAC 
ATGTGTCCAG CTACCAACCA GTGCACAATT GATAAGAACA GGAGGAAGAG CTGTCAGGCC 
TGTCGGCTGC GCAAGTGCTA TGAAGTTGGC ATGATGAAAG GCGGGATACG GAAAGACCGA 
AGAGGAGGAA GAATGTTGAA ACACAAACGC CAGAGAGATG ATGGGGAGGG CAGGAATGAA 
GTGGGGTCCT CTGGAGATGT GAGGACTTCC AGCCTTTGGC CAAGCCCTCT CTTGATTAAG 
CATACTAAGA AGAACAGCCC AGCCTTGTCC CTGACAGCCG ATCAGATGGT CAGTGCCTTG 
TTAGAGGCTG AGCCCCCCAT AATCTATTCC GACTATGACC CATCCAGACC CTTCAGTGAA 
GCTTCGATGA TGGGCTTGCT GACCAACCTT GCTGACAGGG AGCTGGTTCA CATGATCAAC 
TGGGCAAAGA GGGTACCAGG CTTTGTTGAT TTGAGCCTCC ATGATCAGGT CCACCTTCTG 
GAATGTGCTT GGCTAGAGAT CCTGATGATT GGACTTGTCT GGCGCTCCAT GGAGCATCCA 
GGGAAGCTCT TCTTTGCTCC TAACTTGCTC CTAGACAGGA ACCAGGGAAA ATGTGTAGAG 
GGCATCGTGG AGATCTTTGA CATGTTGCTG GCTACGTCAT CTCGATTTCG TATGATGAAT 
CTCCAGGGCG AGGAGTTTGT GTGCCTCAAA TCCATCATTT TGCTTAATTC TGTCATTTGT 
CCGGTACGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs.
Tissue & cell48(1)10-7(2016 Feb)
Chuffa LG,Lupi J?nior LA,da Maia Lima AF

The ESR1 gene is associated with risk for canine mammary tumours.
BMC veterinary research969(2013 Apr)
Borge KS,Melin M,Rivera P,Thoresen SI,Webster MT,von Euler H,Lindblad-Toh K,Lingaas F

Homozygosity of single nucleotide polymorphisms in the 3' region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas.
Animal science journal = Nihon chikusan Gakkaiho82(3)390-5(2011 Jun)
Pathirana IN,Tanaka K,Kawate N,Tsuji M,Hatoya S,Inaba T,Tamada H

Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas.
The Journal of reproduction and development56(4)405-10(2010 Aug)
Pathirana IN,Tanaka K,Kawate N,Tsuji M,Kida K,Hatoya S,Inaba T,Tamada H

ERalpha increases expression and interacts with TERT in cataractous canine lens epithelial cells.
Molecular vision152259-67(2009 Nov)
Colitz CM,Sugimoto Y,Lu P,Barden CA,Thomas-Ahner J,Chandler HL

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ESR1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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