TLR9 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR9
Entrez Gene ID	403502
Full Name	toll like receptor 9
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014121724.2	XP_013977199.1	toll-like receptor 9 isoform X2
XM_014121722.1	XP_013977197.1	toll-like receptor 9 isoform X1
XM_014121722.2	XP_013977197.1	toll-like receptor 9 isoform X1
XM_014121723.1	XP_013977198.1	toll-like receptor 9 isoform X2
XM_014121724.1	XP_013977199.1	toll-like receptor 9 isoform X3
NM_001002998.1	NP_001002998.1	toll-like receptor 9 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100494387	XP_002936533.2
Homo sapiens (human)	TLR9	NP_059138.1
Canis lupus familiaris (dog)	TLR9	NP_001002998.1
Rattus norvegicus (Norway rat)	Tlr9	NP_937764.1
Bos taurus (cattle)	TLR9	NP_898904.1
Danio rerio (zebrafish)	tlr9	NP_001124066.1
Pan troglodytes (chimpanzee)	TLR9	NP_001138338.1
	TLR9	NP_001123903.1
Mus musculus (house mouse)	Tlr9	NP_112455.2

Gene Ontology
Bibliography

Related articles in PubMed

Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.
Melo GD, Silva JE, Grano FG, Homem CG, Machado GF
Parasite immunology36(12)726-31(2014 Dec)

Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.
Mercier E, Peters IR, Farnir F, Lavou? R, Day M, Clercx C, Peeters D
BMC veterinary research10187(2014 Aug)

Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL
BMC immunology1422(2013 May)

Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle.
Schneberger D, Lewis D, Caldwell S, Singh B
International journal of experimental pathology92(1)1-7(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study investigated the pattern of TLR-2, TLR-4 and TLR-9 gene expression in brain, choroid plexus, spleen and lymph node of dogs naturally infected with Leishmania infantum. In the brain, there was only an upregulation of TLR-2, in the choroid plexus, TLR-2 and TLR-9 were both upregulated and peripheral lymphoid organs showed increased TLR-2 and TLR-4 expression.
Title: Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.

These findings do not support a role for non-synonymous SNPs in the TLR 2, 4 and 9 coding regions in the pathogenesis of canine SNA, but do not exclude a role for innate immunity in the pathogenesis of the disease.
Title: Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.

The aim of this study was to investigate the expression of TLR2 and TLR9 in jejunum and colon in dogs naturally infected with Leishmania infantum.
Title: Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.

we studied TLR9 expression in lung extracts from pigs, dogs, and cattle.
Title: Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle.

The amino acid sequence of canine TLR9, its homology with TLR9 from other species, and its expression in various tissues are reported.
Title: Cloning of canine toll-like receptor 9 and its expression in dog tissues.

The following TLR9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe41551	XM_014121723.1 Latest version!	Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe00191	NM_001002998.1 Latest version!	Canis lupus familiaris toll like receptor 9 (TLR9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe41550	XM_014121722.2 Latest version!	Canis lupus familiaris toll like receptor 9 (TLR9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe41552	XM_014121724.2 Latest version!	Canis lupus familiaris toll like receptor 9 (TLR9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OCe41550	XM_014121722.1	Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe41552	XM_014121724.1	Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR9 ( XM_014121723.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977198.1 Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe41551
Clone ID Related Accession (Same CDS sequence)	XM_014121723.1
Accession Version	XM_014121723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	toll-like receptor 9 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACC AGACGGGCAT CCACGCCACC GAGGAGCTGA AGGAGTTCTT CGCCAAGGCA 
CGGGCTGGCT CTGTCCGGCT CATCAAAGTC GTCATCGAGG ATGAGCAGCT TGTGCTGGGT 
GCCTCACGGG AGCTGGCGGG CTGCTGGGAC CAGGACTATG ACAGGACCGT ACTGCCGCTG 
CTGGACGCCC AGGAGCCCTG TTACCTGCTC TACCGCCTGG ACTCACAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTTGC TTGGTCACCT GACAATTCTC CCGTGCGGCT GAAGATGCTG 
TATGCGGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGGG GCGGCCACAT CAAGGATGAG 
CTCTTTGGGA CTGTGAAGGA CGACCTCTCC TTTGCTGGGT ACCAGAAGCA TCTGTCGTCC 
TGTGCAGCGC CCGCCCCACT GACGTCGGCT GAGAGAGAGC TTCAGCAGAT CCGTATTAAT 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAGCACCAGA CCCTGCAGGG CCTCAACTTC 
CCACTGCAGC CGGCAGCCCA GAGGGCACTT CAGCAGCTCA AACAGAAGGC CGTCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACAATTGAGC TGGTGCACAC AGAGCCCACG 
GAAGTGGCCC AGCTGCCCTC AAGGGTTCCC CGAGATGCTG CCCGCTACCA CTTCTTCCTC 
TACAAGCATA CTCATGAGGG TGACCCCCTC GAGTCTGTGG TGTTCATCTA CTCGATGCCA 
GGGTACAAGT GCAGCATCAA GGAGCGCATG CTCTATTCCA GCTGCAAGAG TCGCCTCCTC 
GACTCTGTGG AGCAGGACTT CCAGCTGGAG ATCTCCAAGA AGATTGAGAT TGGCGATGGG 
GCGGAGCTGA CAGCCGAGTT CCTCTACGAC GAGGTGCACC CCAAACAACA TGCCTTCAAG 
CAGGCCTTCG CCAAGCCCAA GGGCCCAGGG GGCAAGCGGG GTCACAAGCG CCTCATCCGC 
GGCCCTGGCG AGAATGGGGA TGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977198.1
CDS	189..1238
Translation	MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASRELAGCWDQDYDRTVLPLLDAQEPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLNFPLQPAAQRALQQLKQKAVNYIQLKLDLERETIELVHTEPTEVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFQLEISKKIEIGDGAELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGDDS

Target ORF information:

RefSeq Version	XM_014121723.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121723.1

ORF Insert Sequence:

ATGGCGCACC AGACGGGCAT CCACGCCACC GAGGAGCTGA AGGAGTTCTT CGCCAAGGCA 
CGGGCTGGCT CTGTCCGGCT CATCAAAGTC GTCATCGAGG ATGAGCAGCT TGTGCTGGGT 
GCCTCACGGG AGCTGGCGGG CTGCTGGGAC CAGGACTATG ACAGGACCGT ACTGCCGCTG 
CTGGACGCCC AGGAGCCCTG TTACCTGCTC TACCGCCTGG ACTCACAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTTGC TTGGTCACCT GACAATTCTC CCGTGCGGCT GAAGATGCTG 
TATGCGGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGGG GCGGCCACAT CAAGGATGAG 
CTCTTTGGGA CTGTGAAGGA CGACCTCTCC TTTGCTGGGT ACCAGAAGCA TCTGTCGTCC 
TGTGCAGCGC CCGCCCCACT GACGTCGGCT GAGAGAGAGC TTCAGCAGAT CCGTATTAAT 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAGCACCAGA CCCTGCAGGG CCTCAACTTC 
CCACTGCAGC CGGCAGCCCA GAGGGCACTT CAGCAGCTCA AACAGAAGGC CGTCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACAATTGAGC TGGTGCACAC AGAGCCCACG 
GAAGTGGCCC AGCTGCCCTC AAGGGTTCCC CGAGATGCTG CCCGCTACCA CTTCTTCCTC 
TACAAGCATA CTCATGAGGG TGACCCCCTC GAGTCTGTGG TGTTCATCTA CTCGATGCCA 
GGGTACAAGT GCAGCATCAA GGAGCGCATG CTCTATTCCA GCTGCAAGAG TCGCCTCCTC 
GACTCTGTGG AGCAGGACTT CCAGCTGGAG ATCTCCAAGA AGATTGAGAT TGGCGATGGG 
GCGGAGCTGA CAGCCGAGTT CCTCTACGAC GAGGTGCACC CCAAACAACA TGCCTTCAAG 
CAGGCCTTCG CCAAGCCCAA GGGCCCAGGG GGCAAGCGGG GTCACAAGCG CCTCATCCGC 
GGCCCTGGCG AGAATGGGGA TGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR9 ( NM_001002998.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002998.1 Canis lupus familiaris toll like receptor 9 (TLR9), mRNA.

Price & Availability↑

CloneID	OCe00191
Clone ID Related Accession (Same CDS sequence)	NM_001002998.1
Accession Version	NM_001002998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 9 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB104899.3. ##Evidence-Data-START## Transcript exon combination :: AB104899.3 [ECO:0000332] ##Evidence-Data-END##

ATGGGCCCCT GCCGTGGCGC CCTGCACCCC CTGTCTCTCC TGGTGCAGGC TGCCGCGCTA 
GCCCTGGCCC TGGCCCAGGG CACCCTGCCT GCCTTCCTGC CCTGTGAGCT CCAGCCCCAT 
GGCCTGGTGA ACTGCAACTG GCTGTTCCTC AAGTCCGTGC CCCGCTTCTC GGCAGCTGCA 
CCCCGCGGTA ACGTCACCAG CCTTTCCTTG TACTCCAACC GCATCCACCA CCTCCATGAC 
TATGACTTTG TCCACTTCGT CCACCTGCGG CGTCTCAATC TCAAGTGGAA CTGCCCGCCC 
GCCAGCCTCA GCCCCATGCA CTTTCCCTGC CACATGACCA TTGAGCCCAA CACCTTCCTG 
GCTGTGCCCA CCCTAGAGGA CCTGAATCTG AGCTATAACA GCATCACGAC TGTGCCCGCC 
CTGCCCAGTT CGCTTGTGTC CCTGTCCCTG AGCCGCACCA ACATCCTGGT GCCGGACCCT 
GCCACCCTGG CAGGCCTTTA TGCCCTGCGC TTCCTGTTCC TGGATGGCAA CTGCTACTAC 
AAGAACCCCT GCCAGCAGGC CCTGCAGGTG GCCCCAGGTG CCCTCCTGGG CCTGGGCAAC 
CTCACACACC TGTCACTCAA GTACAACAAC CTCACCGTGG TGCCGCGGGG CCTGCCCCCC 
AGCCTGGAGT ACCTGCTCTT GTCCTACAAC CACATCATCA CCCTGGCACC TGAGGACCTG 
GCCAATCTGA CTGCCCTGCG TGTCCTCGAT GTGGGTGGGA ACTGTCGCCG CTGTGACCAT 
GCCCGTAACC CCTGCAGGGA GTGCCCCAAG GGCTTCCCCC AGCTGCACCC CAACACCTTC 
GGCCACCTGA ACCACCTCGA AGGCCTGGTG TTGAGGGACA GCTCTCTCTA CAGCCTGGAC 
CCCAGGTGGT TCCATGGCCT GGGCAACCTC ATGGTGCTGG ACCTGAGTGA GAACTTCCTG 
TATGACTGCA TCACCAAAAC CAAAGCCTTC TACGGCCTGG CCCGGCTGCG CAGACTCAAC 
CTGTCCTTCA ATTATCATAA GAAGGTGTCC TTTGCCCACC TGCATCTGGC ATCCTCCTTC 
GGGAGCCTAC TGTCCCTGCA GGAGCTGGAC ATACATGGCA TCTTCTTCCG CTCGCTCAGC 
GAGACCACGC TCCAGTCGCT GGCCCACCTG CCCATGCTCC AGCGTCTGCA TCTGCAGTTG 
AACTTTATCA GCCAGGCCCA GCTCAGCATC TTCGGCGCCT TCCCTGGCCT GCGGTACGTG 
GACTTGTCAG ACAACCGCAT CAGTGGAGCT GCAGAACCCG CGGCTGCCAC AGGGGAGGTA 
GAGGCGGACT GTGGGGAGAG AGTCTGGCCA CAGTCCCGGG ACCTTGCTCT GGGCCCACTG 
GGCACCCCCG GCTCAGAGGC CTTCATGCCG AGCTGCAGGA CCCTCAACTT CACCTTGGAC 
CTGTCTCGGA ACAACCTAGT GACTGTTCAG CCGGAGATGT TTGTCCGGCT GGCGCGCCTC 
CAGTGCCTGG GCCTGAGCCA CAACAGCATC TCGCAGGCGG TCAATGGCTC GCAGTTCGTG 
CCTCTGAGCA ACCTGCGGGT GCTGGACCTG TCCCATAACA AGCTGGACCT GTACCACGGG 
CGCTCGTTCA CGGAGCTGCC GCGGCTGGAG GCCTTGGACC TCAGCTACAA CAGCCAGCCC 
TTCAGCATGC GGGGCGTGGG CCACAATCTC AGCTTTGTGG CACAGCTGCC GGCCCTGCGC 
TACCTCAGCC TGGCGCACAA TGGCATCCAC AGCCGCGTGT CCCAGCAGCT CCGCAGCGCC 
TCGCTCCGGG CCCTGGACTT CAGTGGCAAT ACCCCGAGCC TGATGTGGGC CGAGGGAGAC 
CTCTATCTCC GCTTCTTCCA AGGCCTGAGA AGCCTGGTTC AGCTGGACCT GTCCCAGAAT 
CGCCTGCATA CCCTCCTGCC ACGCAACCTG GACAACCTCC CCAAGAGCCT GCGGCTCCTG 
CGGCTCCGTG ACAATTACCT GGCTTTCTTC AACTGGAGCA GCCTGGCCCT CCTACCCAAG 
CTGGAAGCCC TGGACCTGGC GGGAAACCAG CTGAAGGCCC TGAGCAATGG CAGCTTGCCC 
AACGGCACCC AGCTCCAGAG GCTGGACCTC AGCGGCAACA GCATCGGCTT CGTGGTCCCC 
GGCTTTTTTG CCCTGGCCGT GAGGCTTCGA GAGCTCAACC TCAGCGCCAA CGCCCTCAAG 
ACGGCGGAGC CCTCCTGGTT TGGTTCCCTG GCGGGTGCCC TGAAAGTCCT AGACGTGACC 
GCCAACCCCT TGCATTGCGC TTGCGGCGCA ACCTTCGTGG ACTTCTTGCT GGAGGTGCAG 
GCTGCGGTGC CCGGCCTGCC TAGCCGTGTC AAGTGCGGCA GCCCGGGCCA GCTCCAGGGC 
CGCAGCATCT TCGCACAGGA CCTGCGCCTC TGCCTGGACG AAGCGCTCTC CTGGGTCTGT 
TTCAGCCTCT CGCTGCTGGC TGTGGCCCTG AGCCTGGCTG TGCCCATGCT GCACCAGCTC 
TGTGGCTGGG ACCTCTGGTA CTGCTTCCAC CTGTGCCTGG CCTGGCTGCC CCGGCGGGGG 
CGGCGGCGGG GTGTGGATGC CCTGGCCTAC GACGCCTTCG TGGTCTTCGA CAAGGCGCAG 
AGCTCGGTGG CGGACTGGGT GTACAATGAG CTGCGGGTAC AGCTAGAGGA GCGCCGTGGG 
CGCCGGGCGC TACGCCTGTG TCTGGAGGAA CGTGACTGGG TACCCGGCAA AACCCTCTTC 
GAGAACCTCT GGGCCTCAGT TTACAGCAGC CGCAAGACGC TGTTTGTGCT GGCCCGCACG 
GACAGAGTCA GCGGCCTCCT GCGTGCCAGC TTCCTGCTGG CCCAACAGCG CCTGCTGGAG 
GACCGCAAGG ACGTCGTGGT GCTGGTGATC CTGTGCCCCG ACGCCCACCG CTCCCGCTAT 
GTGCGGCTGC GCCAGCGCCT CTGCCGCCAG AGTGTCCTCC TCTGGCCCCA CCAGCCCAGT 
GGCCAGCGCA GCTTCTGGGC CCAGCTGGGC ACGGCCCTGA CCAGGGACAA CCGCCACTTC 
TACAACCAGA ACTTCTGCCG GGGCCCCACG ACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002998.1
CDS	153..3251
Translation	MGPCRGALHPLSLLVQAAALALALAQGTLPAFLPCELQPHGLVNCNWLFLKSVPRFSAAAPRGNVTSLSLYSNRIHHLHDYDFVHFVHLRRLNLKWNCPPASLSPMHFPCHMTIEPNTFLAVPTLEDLNLSYNSITTVPALPSSLVSLSLSRTNILVPDPATLAGLYALRFLFLDGNCYYKNPCQQALQVAPGALLGLGNLTHLSLKYNNLTVVPRGLPPSLEYLLLSYNHIITLAPEDLANLTALRVLDVGGNCRRCDHARNPCRECPKGFPQLHPNTFGHLNHLEGLVLRDSSLYSLDPRWFHGLGNLMVLDLSENFLYDCITKTKAFYGLARLRRLNLSFNYHKKVSFAHLHLASSFGSLLSLQELDIHGIFFRSLSETTLQSLAHLPMLQRLHLQLNFISQAQLSIFGAFPGLRYVDLSDNRISGAAEPAAATGEVEADCGERVWPQSRDLALGPLGTPGSEAFMPSCRTLNFTLDLSRNNLVTVQPEMFVRLARLQCLGLSHNSISQAVNGSQFVPLSNLRVLDLSHNKLDLYHGRSFTELPRLEALDLSYNSQPFSMRGVGHNLSFVAQLPALRYLSLAHNGIHSRVSQQLRSASLRALDFSGNTPSLMWAEGDLYLRFFQGLRSLVQLDLSQNRLHTLLPRNLDNLPKSLRLLRLRDNYLAFFNWSSLALLPKLEALDLAGNQLKALSNGSLPNGTQLQRLDLSGNSIGFVVPGFFALAVRLRELNLSANALKTAEPSWFGSLAGALKVLDVTANPLHCACGATFVDFLLEVQAAVPGLPSRVKCGSPGQLQGRSIFAQDLRLCLDEALSWVCFSLSLLAVALSLAVPMLHQLCGWDLWYCFHLCLAWLPRRGRRRGVDALAYDAFVVFDKAQSSVADWVYNELRVQLEERRGRRALRLCLEERDWVPGKTLFENLWASVYSSRKTLFVLARTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILCPDAHRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGTALTRDNRHFYNQNFCRGPTTA

Target ORF information:

RefSeq Version	NM_001002998.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 9 (TLR9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002998.1

ORF Insert Sequence:

ATGGGCCCCT GCCGTGGCGC CCTGCACCCC CTGTCTCTCC TGGTGCAGGC TGCCGCGCTA 
GCCCTGGCCC TGGCCCAGGG CACCCTGCCT GCCTTCCTGC CCTGTGAGCT CCAGCCCCAT 
GGCCTGGTGA ACTGCAACTG GCTGTTCCTC AAGTCCGTGC CCCGCTTCTC GGCAGCTGCA 
CCCCGCGGTA ACGTCACCAG CCTTTCCTTG TACTCCAACC GCATCCACCA CCTCCATGAC 
TATGACTTTG TCCACTTCGT CCACCTGCGG CGTCTCAATC TCAAGTGGAA CTGCCCGCCC 
GCCAGCCTCA GCCCCATGCA CTTTCCCTGC CACATGACCA TTGAGCCCAA CACCTTCCTG 
GCTGTGCCCA CCCTAGAGGA CCTGAATCTG AGCTATAACA GCATCACGAC TGTGCCCGCC 
CTGCCCAGTT CGCTTGTGTC CCTGTCCCTG AGCCGCACCA ACATCCTGGT GCCGGACCCT 
GCCACCCTGG CAGGCCTTTA TGCCCTGCGC TTCCTGTTCC TGGATGGCAA CTGCTACTAC 
AAGAACCCCT GCCAGCAGGC CCTGCAGGTG GCCCCAGGTG CCCTCCTGGG CCTGGGCAAC 
CTCACACACC TGTCACTCAA GTACAACAAC CTCACCGTGG TGCCGCGGGG CCTGCCCCCC 
AGCCTGGAGT ACCTGCTCTT GTCCTACAAC CACATCATCA CCCTGGCACC TGAGGACCTG 
GCCAATCTGA CTGCCCTGCG TGTCCTCGAT GTGGGTGGGA ACTGTCGCCG CTGTGACCAT 
GCCCGTAACC CCTGCAGGGA GTGCCCCAAG GGCTTCCCCC AGCTGCACCC CAACACCTTC 
GGCCACCTGA ACCACCTCGA AGGCCTGGTG TTGAGGGACA GCTCTCTCTA CAGCCTGGAC 
CCCAGGTGGT TCCATGGCCT GGGCAACCTC ATGGTGCTGG ACCTGAGTGA GAACTTCCTG 
TATGACTGCA TCACCAAAAC CAAAGCCTTC TACGGCCTGG CCCGGCTGCG CAGACTCAAC 
CTGTCCTTCA ATTATCATAA GAAGGTGTCC TTTGCCCACC TGCATCTGGC ATCCTCCTTC 
GGGAGCCTAC TGTCCCTGCA GGAGCTGGAC ATACATGGCA TCTTCTTCCG CTCGCTCAGC 
GAGACCACGC TCCAGTCGCT GGCCCACCTG CCCATGCTCC AGCGTCTGCA TCTGCAGTTG 
AACTTTATCA GCCAGGCCCA GCTCAGCATC TTCGGCGCCT TCCCTGGCCT GCGGTACGTG 
GACTTGTCAG ACAACCGCAT CAGTGGAGCT GCAGAACCCG CGGCTGCCAC AGGGGAGGTA 
GAGGCGGACT GTGGGGAGAG AGTCTGGCCA CAGTCCCGGG ACCTTGCTCT GGGCCCACTG 
GGCACCCCCG GCTCAGAGGC CTTCATGCCG AGCTGCAGGA CCCTCAACTT CACCTTGGAC 
CTGTCTCGGA ACAACCTAGT GACTGTTCAG CCGGAGATGT TTGTCCGGCT GGCGCGCCTC 
CAGTGCCTGG GCCTGAGCCA CAACAGCATC TCGCAGGCGG TCAATGGCTC GCAGTTCGTG 
CCTCTGAGCA ACCTGCGGGT GCTGGACCTG TCCCATAACA AGCTGGACCT GTACCACGGG 
CGCTCGTTCA CGGAGCTGCC GCGGCTGGAG GCCTTGGACC TCAGCTACAA CAGCCAGCCC 
TTCAGCATGC GGGGCGTGGG CCACAATCTC AGCTTTGTGG CACAGCTGCC GGCCCTGCGC 
TACCTCAGCC TGGCGCACAA TGGCATCCAC AGCCGCGTGT CCCAGCAGCT CCGCAGCGCC 
TCGCTCCGGG CCCTGGACTT CAGTGGCAAT ACCCCGAGCC TGATGTGGGC CGAGGGAGAC 
CTCTATCTCC GCTTCTTCCA AGGCCTGAGA AGCCTGGTTC AGCTGGACCT GTCCCAGAAT 
CGCCTGCATA CCCTCCTGCC ACGCAACCTG GACAACCTCC CCAAGAGCCT GCGGCTCCTG 
CGGCTCCGTG ACAATTACCT GGCTTTCTTC AACTGGAGCA GCCTGGCCCT CCTACCCAAG 
CTGGAAGCCC TGGACCTGGC GGGAAACCAG CTGAAGGCCC TGAGCAATGG CAGCTTGCCC 
AACGGCACCC AGCTCCAGAG GCTGGACCTC AGCGGCAACA GCATCGGCTT CGTGGTCCCC 
GGCTTTTTTG CCCTGGCCGT GAGGCTTCGA GAGCTCAACC TCAGCGCCAA CGCCCTCAAG 
ACGGCGGAGC CCTCCTGGTT TGGTTCCCTG GCGGGTGCCC TGAAAGTCCT AGACGTGACC 
GCCAACCCCT TGCATTGCGC TTGCGGCGCA ACCTTCGTGG ACTTCTTGCT GGAGGTGCAG 
GCTGCGGTGC CCGGCCTGCC TAGCCGTGTC AAGTGCGGCA GCCCGGGCCA GCTCCAGGGC 
CGCAGCATCT TCGCACAGGA CCTGCGCCTC TGCCTGGACG AAGCGCTCTC CTGGGTCTGT 
TTCAGCCTCT CGCTGCTGGC TGTGGCCCTG AGCCTGGCTG TGCCCATGCT GCACCAGCTC 
TGTGGCTGGG ACCTCTGGTA CTGCTTCCAC CTGTGCCTGG CCTGGCTGCC CCGGCGGGGG 
CGGCGGCGGG GTGTGGATGC CCTGGCCTAC GACGCCTTCG TGGTCTTCGA CAAGGCGCAG 
AGCTCGGTGG CGGACTGGGT GTACAATGAG CTGCGGGTAC AGCTAGAGGA GCGCCGTGGG 
CGCCGGGCGC TACGCCTGTG TCTGGAGGAA CGTGACTGGG TACCCGGCAA AACCCTCTTC 
GAGAACCTCT GGGCCTCAGT TTACAGCAGC CGCAAGACGC TGTTTGTGCT GGCCCGCACG 
GACAGAGTCA GCGGCCTCCT GCGTGCCAGC TTCCTGCTGG CCCAACAGCG CCTGCTGGAG 
GACCGCAAGG ACGTCGTGGT GCTGGTGATC CTGTGCCCCG ACGCCCACCG CTCCCGCTAT 
GTGCGGCTGC GCCAGCGCCT CTGCCGCCAG AGTGTCCTCC TCTGGCCCCA CCAGCCCAGT 
GGCCAGCGCA GCTTCTGGGC CCAGCTGGGC ACGGCCCTGA CCAGGGACAA CCGCCACTTC 
TACAACCAGA ACTTCTGCCG GGGCCCCACG ACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR9 ( XM_014121722.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977197.1 Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41550
Clone ID Related Accession (Same CDS sequence)	XM_014121722.1 , XM_014121722.2
Accession Version	XM_014121722.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	toll-like receptor 9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCCT GCCGTGGCGC CCTGCACCCC CTGTCTCTCC TGGTGCAGGC TGCCGCGCTA 
GCCCTGGCCC TGGCCCAGGG CACCCTGCCT GCCTTCCTGC CCTGTGAGCT CCAGCCCCAT 
GGCCTGGTGA ACTGCAACTG GCTGTTCCTC AAGTCCGTGC CCCGCTTCTC GGCAGCTGCA 
CCCCGCGGTA ACGTCACCAG CCTTTCCTTG TACTCCAACC GCATCCACCA CCTCCATGAC 
TATGACTTTG TCCACTTCGT CCACCTGCGG CGTCTCAATC TCAAGTGGAA CTGCCCGCCC 
GCCAGCCTCA GCCCCATGCA CTTTCCCTGT CACATGACCA TTGAGCCCAA CACCTTCCTG 
GCTGTGCCCA CCCTAGAGGA CCTGAATCTG AGCTATAACA GCATCACGAC TGTGCCCGCC 
CTGCCCAGTT CGCTTGTGTC CCTGTCCCTG AGCCGCACCA ACATCCTGGT GCTGGACCCT 
GCCACCCTGG CAGGCCTTTA TGCCCTGCGC TTCCTGTTCC TGGATGGCAA CTGCTACTAC 
AAGAACCCCT GCCAGCAGGC CCTGCAGGTG GCCCCAGGTG CCCTCCTGGG CCTGGGCAAC 
CTCACACACC TGTCACTCAA GTACAACAAC CTCACCGTGG TGCCGCGGGG CCTGCCCCCC 
AGCCTGGAGT ACCTGCTCTT GTCCTACAAC CACATCATCA CCCTGGCACC TGAGGACCTG 
GCCAATCTGA CTGCCCTGCG TGTCCTCGAT GTGGGTGGGA ACTGTCGCCG CTGTGACCAT 
GCCCGTAACC CCTGCAGGGA GTGCCCCAAG GGCTTCCCCC AGCTGCACCC CAACACCTTC 
GGCCACCTGA GCCACCTCGA AGGCCTGGTG TTGAGGGACA GCTCTCTCTA CAGCCTGGAC 
CCCAGGTGGT TCCATGGCCT GGGCAACCTC ATGGTGCTGG ACCTGAGTGA GAACTTCCTG 
TATGACTGCA TCACCAAAAC CAAAGCCTTC TACGGCCTGG CCCGGCTGCG CAGACTCAAC 
CTGTCCTTCA ATTATCATAA GAAGGTGTCC TTTGCCCACC TGCATCTGGC ATCCTCCTTC 
GGGAGCCTAC TGTCCCTGCA GGAGCTGGAC ATACATGGCA TCTTCTTCCG CTCGCTCAGC 
AAGACCACGC TCCAGTCGCT GGCCCACCTG CCCATGCTCC AGCGTCTGCA TCTGCAGTTG 
AACTTTATCA GCCAGGCCCA GCTCAGCATC TTCGGCGCCT TCCCTGGACT GCGGTACGTG 
GACTTGTCAG ACAACCGCAT CAGTGGAGCT GCAGAGCCCG CGGCTGCCAC AGGGGAGGTA 
GAGGCAGACT GTGGGGAGAG AGTCTGGCCA CAGTCCCGGG ACCTTGCTCT GGGCCCACTG 
GGCACCCCCG GCTCAGAGGC CTTCATGCCG AGCTGCAGGA CCCTCAACTT CACCTTGGAC 
CTGTCTCGGA ACAACCTAGT GACTGTTCAG CCGGAGATGT TTGTCCGGCT GGCGCGCCTC 
CAGTGCCTGG GCCTGAGCCA CAACAGCATC TCGCAGGCGG TCAATGGCTC GCAGTTCGTG 
CCTCTGAGCA ACCTGCGGGT GCTGGACCTG TCCCATAACA AGCTGGACCT GTACCACGGG 
CGCTCGTTCA CGGAGCTGCC GCGGCTGGAG GCCTTGGACC TCAGCTACAA CAGCCAGCCC 
TTCAGCATGC GGGGCGTGGG CCACAATCTC AGCTTTGTGG CACAGCTGCC AGCCCTGCGC 
TACCTCAGCC TGGCGCACAA TGGCATCCAC AGCCGCGTGT CCCAGCAGCT CCGCAGCGCC 
TCGCTCCGGG CCCTGGACTT CAGTGGCAAT ACCCTGAGCC AGATGTGGGC CGAGGGAGAC 
CTCTATCTCC GCTTCTTCCA AGGCCTGAGA AGCCTGGTTC AGCTGGACCT GTCCCAGAAT 
CGCCTGCATA CCCTCCTGCC ACGCAACCTG GACAACCTCC CCAAGAGCCT GCGGCTCCTG 
CGGCTCCGTG ACAATTACCT GGCTTTCTTC AACTGGAGCA GCCTGGCCCT CCTACCCAAG 
CTGGAAGCCC TGGACCTGGC GGGAAACCAG CTGAAGGCCC TGAGCAATGG CAGCTTGCCC 
AACGGCACCC AGCTCCAGAG GCTGGACCTC AGCGGCAACA GCATCGGCTT CGTGGTCCCC 
AGCTTTTTTG CCCTGGCCGT GAGGCTTCGA GAGCTCAACC TCAGCGCCAA CGCCCTCAAG 
ACGGTGGAGC CCTCCTGGTT TGGTTCCCTG GCGGGTGCCC TGAAAGTCCT AGACGTGACC 
GCCAACCCCT TGCATTGCGC TTGCGGCGCA ACCTTCGTGG ACTTCTTGCT GGAGGTGCAG 
GCTGCGGTGC CCGGCCTGCC TAGCCGTGTC AAGTGCGGCA GCCCGGGCCA GCTCCAGGGC 
CGCAGCATCT TCGCACAGGA CCTGCGCCTC TGCCTGGACG AAGCGCTCTC CTGGGTCTGT 
TTCAGCCTCT CGCTGCTGGC TGTGGCCCTG AGCCTGGCTG TGCCCATGCT GCACCAGCTC 
TGTGGCTGGG ACCTCTGGTA CTGCTTCCAC CTGTGCCTGG CCTGGCTGCC CCGGCGGGGG 
CGGCGGCGGG GTGTGGATGC CCTGGCCTAT GACGCCTTCG TGGTCTTCGA CAAGGCGCAG 
AGCTCGGTGG CGGACTGGGT GTACAATGAG CTGCGGGTAC AGCTAGAGGA GCGCCGTGGG 
CGCCGGGCGC TACGCCTGTG TCTGGAGGAA CGTGACTGGG TACCCGGCAA AACCCTCTTT 
GAGAACCTCT GGGCCTCAGT TTACAGCAGC CGCAAGACGC TGTTTGTGCT GGCCCGCACG 
GACAGAGTCA GCGGCCTCCT GCGTGCCAGC TTCCTGCTGG CCCAACAGCG CCTGCTGGAG 
GACCGCAAGG ACGTCGTGGT GCTGGTGATC CTGTGCCCCG ACGCCCACCG CTCCCGCTAT 
GTGCGGCTGC GCCAGCGCCT CTGCCGCCAG AGTGTCCTCC TCTGGCCCCA CCAGCCCAGT 
GGCCAGCGCA GCTTCTGGGC CCAGCTGGGC ACGGCCCTGA CCAGGGACAA CCGCCACTTC 
TACAACCAGA ACTTCTGCCG GGGCCCCACG ACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977197.1
CDS	3427..6525
Translation	MGPCRGALHPLSLLVQAAALALALAQGTLPAFLPCELQPHGLVNCNWLFLKSVPRFSAAAPRGNVTSLSLYSNRIHHLHDYDFVHFVHLRRLNLKWNCPPASLSPMHFPCHMTIEPNTFLAVPTLEDLNLSYNSITTVPALPSSLVSLSLSRTNILVLDPATLAGLYALRFLFLDGNCYYKNPCQQALQVAPGALLGLGNLTHLSLKYNNLTVVPRGLPPSLEYLLLSYNHIITLAPEDLANLTALRVLDVGGNCRRCDHARNPCRECPKGFPQLHPNTFGHLSHLEGLVLRDSSLYSLDPRWFHGLGNLMVLDLSENFLYDCITKTKAFYGLARLRRLNLSFNYHKKVSFAHLHLASSFGSLLSLQELDIHGIFFRSLSKTTLQSLAHLPMLQRLHLQLNFISQAQLSIFGAFPGLRYVDLSDNRISGAAEPAAATGEVEADCGERVWPQSRDLALGPLGTPGSEAFMPSCRTLNFTLDLSRNNLVTVQPEMFVRLARLQCLGLSHNSISQAVNGSQFVPLSNLRVLDLSHNKLDLYHGRSFTELPRLEALDLSYNSQPFSMRGVGHNLSFVAQLPALRYLSLAHNGIHSRVSQQLRSASLRALDFSGNTLSQMWAEGDLYLRFFQGLRSLVQLDLSQNRLHTLLPRNLDNLPKSLRLLRLRDNYLAFFNWSSLALLPKLEALDLAGNQLKALSNGSLPNGTQLQRLDLSGNSIGFVVPSFFALAVRLRELNLSANALKTVEPSWFGSLAGALKVLDVTANPLHCACGATFVDFLLEVQAAVPGLPSRVKCGSPGQLQGRSIFAQDLRLCLDEALSWVCFSLSLLAVALSLAVPMLHQLCGWDLWYCFHLCLAWLPRRGRRRGVDALAYDAFVVFDKAQSSVADWVYNELRVQLEERRGRRALRLCLEERDWVPGKTLFENLWASVYSSRKTLFVLARTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILCPDAHRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGTALTRDNRHFYNQNFCRGPTTA

Target ORF information:

RefSeq Version	XM_014121722.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121722.1

ORF Insert Sequence:

ATGGGCCCCT GCCGTGGCGC CCTGCACCCC CTGTCTCTCC TGGTGCAGGC TGCCGCGCTA 
GCCCTGGCCC TGGCCCAGGG CACCCTGCCT GCCTTCCTGC CCTGTGAGCT CCAGCCCCAT 
GGCCTGGTGA ACTGCAACTG GCTGTTCCTC AAGTCCGTGC CCCGCTTCTC GGCAGCTGCA 
CCCCGCGGTA ACGTCACCAG CCTTTCCTTG TACTCCAACC GCATCCACCA CCTCCATGAC 
TATGACTTTG TCCACTTCGT CCACCTGCGG CGTCTCAATC TCAAGTGGAA CTGCCCGCCC 
GCCAGCCTCA GCCCCATGCA CTTTCCCTGT CACATGACCA TTGAGCCCAA CACCTTCCTG 
GCTGTGCCCA CCCTAGAGGA CCTGAATCTG AGCTATAACA GCATCACGAC TGTGCCCGCC 
CTGCCCAGTT CGCTTGTGTC CCTGTCCCTG AGCCGCACCA ACATCCTGGT GCTGGACCCT 
GCCACCCTGG CAGGCCTTTA TGCCCTGCGC TTCCTGTTCC TGGATGGCAA CTGCTACTAC 
AAGAACCCCT GCCAGCAGGC CCTGCAGGTG GCCCCAGGTG CCCTCCTGGG CCTGGGCAAC 
CTCACACACC TGTCACTCAA GTACAACAAC CTCACCGTGG TGCCGCGGGG CCTGCCCCCC 
AGCCTGGAGT ACCTGCTCTT GTCCTACAAC CACATCATCA CCCTGGCACC TGAGGACCTG 
GCCAATCTGA CTGCCCTGCG TGTCCTCGAT GTGGGTGGGA ACTGTCGCCG CTGTGACCAT 
GCCCGTAACC CCTGCAGGGA GTGCCCCAAG GGCTTCCCCC AGCTGCACCC CAACACCTTC 
GGCCACCTGA GCCACCTCGA AGGCCTGGTG TTGAGGGACA GCTCTCTCTA CAGCCTGGAC 
CCCAGGTGGT TCCATGGCCT GGGCAACCTC ATGGTGCTGG ACCTGAGTGA GAACTTCCTG 
TATGACTGCA TCACCAAAAC CAAAGCCTTC TACGGCCTGG CCCGGCTGCG CAGACTCAAC 
CTGTCCTTCA ATTATCATAA GAAGGTGTCC TTTGCCCACC TGCATCTGGC ATCCTCCTTC 
GGGAGCCTAC TGTCCCTGCA GGAGCTGGAC ATACATGGCA TCTTCTTCCG CTCGCTCAGC 
AAGACCACGC TCCAGTCGCT GGCCCACCTG CCCATGCTCC AGCGTCTGCA TCTGCAGTTG 
AACTTTATCA GCCAGGCCCA GCTCAGCATC TTCGGCGCCT TCCCTGGACT GCGGTACGTG 
GACTTGTCAG ACAACCGCAT CAGTGGAGCT GCAGAGCCCG CGGCTGCCAC AGGGGAGGTA 
GAGGCAGACT GTGGGGAGAG AGTCTGGCCA CAGTCCCGGG ACCTTGCTCT GGGCCCACTG 
GGCACCCCCG GCTCAGAGGC CTTCATGCCG AGCTGCAGGA CCCTCAACTT CACCTTGGAC 
CTGTCTCGGA ACAACCTAGT GACTGTTCAG CCGGAGATGT TTGTCCGGCT GGCGCGCCTC 
CAGTGCCTGG GCCTGAGCCA CAACAGCATC TCGCAGGCGG TCAATGGCTC GCAGTTCGTG 
CCTCTGAGCA ACCTGCGGGT GCTGGACCTG TCCCATAACA AGCTGGACCT GTACCACGGG 
CGCTCGTTCA CGGAGCTGCC GCGGCTGGAG GCCTTGGACC TCAGCTACAA CAGCCAGCCC 
TTCAGCATGC GGGGCGTGGG CCACAATCTC AGCTTTGTGG CACAGCTGCC AGCCCTGCGC 
TACCTCAGCC TGGCGCACAA TGGCATCCAC AGCCGCGTGT CCCAGCAGCT CCGCAGCGCC 
TCGCTCCGGG CCCTGGACTT CAGTGGCAAT ACCCTGAGCC AGATGTGGGC CGAGGGAGAC 
CTCTATCTCC GCTTCTTCCA AGGCCTGAGA AGCCTGGTTC AGCTGGACCT GTCCCAGAAT 
CGCCTGCATA CCCTCCTGCC ACGCAACCTG GACAACCTCC CCAAGAGCCT GCGGCTCCTG 
CGGCTCCGTG ACAATTACCT GGCTTTCTTC AACTGGAGCA GCCTGGCCCT CCTACCCAAG 
CTGGAAGCCC TGGACCTGGC GGGAAACCAG CTGAAGGCCC TGAGCAATGG CAGCTTGCCC 
AACGGCACCC AGCTCCAGAG GCTGGACCTC AGCGGCAACA GCATCGGCTT CGTGGTCCCC 
AGCTTTTTTG CCCTGGCCGT GAGGCTTCGA GAGCTCAACC TCAGCGCCAA CGCCCTCAAG 
ACGGTGGAGC CCTCCTGGTT TGGTTCCCTG GCGGGTGCCC TGAAAGTCCT AGACGTGACC 
GCCAACCCCT TGCATTGCGC TTGCGGCGCA ACCTTCGTGG ACTTCTTGCT GGAGGTGCAG 
GCTGCGGTGC CCGGCCTGCC TAGCCGTGTC AAGTGCGGCA GCCCGGGCCA GCTCCAGGGC 
CGCAGCATCT TCGCACAGGA CCTGCGCCTC TGCCTGGACG AAGCGCTCTC CTGGGTCTGT 
TTCAGCCTCT CGCTGCTGGC TGTGGCCCTG AGCCTGGCTG TGCCCATGCT GCACCAGCTC 
TGTGGCTGGG ACCTCTGGTA CTGCTTCCAC CTGTGCCTGG CCTGGCTGCC CCGGCGGGGG 
CGGCGGCGGG GTGTGGATGC CCTGGCCTAT GACGCCTTCG TGGTCTTCGA CAAGGCGCAG 
AGCTCGGTGG CGGACTGGGT GTACAATGAG CTGCGGGTAC AGCTAGAGGA GCGCCGTGGG 
CGCCGGGCGC TACGCCTGTG TCTGGAGGAA CGTGACTGGG TACCCGGCAA AACCCTCTTT 
GAGAACCTCT GGGCCTCAGT TTACAGCAGC CGCAAGACGC TGTTTGTGCT GGCCCGCACG 
GACAGAGTCA GCGGCCTCCT GCGTGCCAGC TTCCTGCTGG CCCAACAGCG CCTGCTGGAG 
GACCGCAAGG ACGTCGTGGT GCTGGTGATC CTGTGCCCCG ACGCCCACCG CTCCCGCTAT 
GTGCGGCTGC GCCAGCGCCT CTGCCGCCAG AGTGTCCTCC TCTGGCCCCA CCAGCCCAGT 
GGCCAGCGCA GCTTCTGGGC CCAGCTGGGC ACGGCCCTGA CCAGGGACAA CCGCCACTTC 
TACAACCAGA ACTTCTGCCG GGGCCCCACG ACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR9 ( XM_014121722.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977197.1 Canis lupus familiaris toll like receptor 9 (TLR9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41550
Clone ID Related Accession (Same CDS sequence)	XM_014121722.1 , XM_014121722.2
Accession Version	XM_014121722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCCT GCCGTGGCGC CCTGCACCCC CTGTCTCTCC TGGTGCAGGC TGCCGCGCTA 
GCCCTGGCCC TGGCCCAGGG CACCCTGCCT GCCTTCCTGC CCTGTGAGCT CCAGCCCCAT 
GGCCTGGTGA ACTGCAACTG GCTGTTCCTC AAGTCCGTGC CCCGCTTCTC GGCAGCTGCA 
CCCCGCGGTA ACGTCACCAG CCTTTCCTTG TACTCCAACC GCATCCACCA CCTCCATGAC 
TATGACTTTG TCCACTTCGT CCACCTGCGG CGTCTCAATC TCAAGTGGAA CTGCCCGCCC 
GCCAGCCTCA GCCCCATGCA CTTTCCCTGT CACATGACCA TTGAGCCCAA CACCTTCCTG 
GCTGTGCCCA CCCTAGAGGA CCTGAATCTG AGCTATAACA GCATCACGAC TGTGCCCGCC 
CTGCCCAGTT CGCTTGTGTC CCTGTCCCTG AGCCGCACCA ACATCCTGGT GCTGGACCCT 
GCCACCCTGG CAGGCCTTTA TGCCCTGCGC TTCCTGTTCC TGGATGGCAA CTGCTACTAC 
AAGAACCCCT GCCAGCAGGC CCTGCAGGTG GCCCCAGGTG CCCTCCTGGG CCTGGGCAAC 
CTCACACACC TGTCACTCAA GTACAACAAC CTCACCGTGG TGCCGCGGGG CCTGCCCCCC 
AGCCTGGAGT ACCTGCTCTT GTCCTACAAC CACATCATCA CCCTGGCACC TGAGGACCTG 
GCCAATCTGA CTGCCCTGCG TGTCCTCGAT GTGGGTGGGA ACTGTCGCCG CTGTGACCAT 
GCCCGTAACC CCTGCAGGGA GTGCCCCAAG GGCTTCCCCC AGCTGCACCC CAACACCTTC 
GGCCACCTGA GCCACCTCGA AGGCCTGGTG TTGAGGGACA GCTCTCTCTA CAGCCTGGAC 
CCCAGGTGGT TCCATGGCCT GGGCAACCTC ATGGTGCTGG ACCTGAGTGA GAACTTCCTG 
TATGACTGCA TCACCAAAAC CAAAGCCTTC TACGGCCTGG CCCGGCTGCG CAGACTCAAC 
CTGTCCTTCA ATTATCATAA GAAGGTGTCC TTTGCCCACC TGCATCTGGC ATCCTCCTTC 
GGGAGCCTAC TGTCCCTGCA GGAGCTGGAC ATACATGGCA TCTTCTTCCG CTCGCTCAGC 
AAGACCACGC TCCAGTCGCT GGCCCACCTG CCCATGCTCC AGCGTCTGCA TCTGCAGTTG 
AACTTTATCA GCCAGGCCCA GCTCAGCATC TTCGGCGCCT TCCCTGGACT GCGGTACGTG 
GACTTGTCAG ACAACCGCAT CAGTGGAGCT GCAGAGCCCG CGGCTGCCAC AGGGGAGGTA 
GAGGCAGACT GTGGGGAGAG AGTCTGGCCA CAGTCCCGGG ACCTTGCTCT GGGCCCACTG 
GGCACCCCCG GCTCAGAGGC CTTCATGCCG AGCTGCAGGA CCCTCAACTT CACCTTGGAC 
CTGTCTCGGA ACAACCTAGT GACTGTTCAG CCGGAGATGT TTGTCCGGCT GGCGCGCCTC 
CAGTGCCTGG GCCTGAGCCA CAACAGCATC TCGCAGGCGG TCAATGGCTC GCAGTTCGTG 
CCTCTGAGCA ACCTGCGGGT GCTGGACCTG TCCCATAACA AGCTGGACCT GTACCACGGG 
CGCTCGTTCA CGGAGCTGCC GCGGCTGGAG GCCTTGGACC TCAGCTACAA CAGCCAGCCC 
TTCAGCATGC GGGGCGTGGG CCACAATCTC AGCTTTGTGG CACAGCTGCC AGCCCTGCGC 
TACCTCAGCC TGGCGCACAA TGGCATCCAC AGCCGCGTGT CCCAGCAGCT CCGCAGCGCC 
TCGCTCCGGG CCCTGGACTT CAGTGGCAAT ACCCTGAGCC AGATGTGGGC CGAGGGAGAC 
CTCTATCTCC GCTTCTTCCA AGGCCTGAGA AGCCTGGTTC AGCTGGACCT GTCCCAGAAT 
CGCCTGCATA CCCTCCTGCC ACGCAACCTG GACAACCTCC CCAAGAGCCT GCGGCTCCTG 
CGGCTCCGTG ACAATTACCT GGCTTTCTTC AACTGGAGCA GCCTGGCCCT CCTACCCAAG 
CTGGAAGCCC TGGACCTGGC GGGAAACCAG CTGAAGGCCC TGAGCAATGG CAGCTTGCCC 
AACGGCACCC AGCTCCAGAG GCTGGACCTC AGCGGCAACA GCATCGGCTT CGTGGTCCCC 
AGCTTTTTTG CCCTGGCCGT GAGGCTTCGA GAGCTCAACC TCAGCGCCAA CGCCCTCAAG 
ACGGTGGAGC CCTCCTGGTT TGGTTCCCTG GCGGGTGCCC TGAAAGTCCT AGACGTGACC 
GCCAACCCCT TGCATTGCGC TTGCGGCGCA ACCTTCGTGG ACTTCTTGCT GGAGGTGCAG 
GCTGCGGTGC CCGGCCTGCC TAGCCGTGTC AAGTGCGGCA GCCCGGGCCA GCTCCAGGGC 
CGCAGCATCT TCGCACAGGA CCTGCGCCTC TGCCTGGACG AAGCGCTCTC CTGGGTCTGT 
TTCAGCCTCT CGCTGCTGGC TGTGGCCCTG AGCCTGGCTG TGCCCATGCT GCACCAGCTC 
TGTGGCTGGG ACCTCTGGTA CTGCTTCCAC CTGTGCCTGG CCTGGCTGCC CCGGCGGGGG 
CGGCGGCGGG GTGTGGATGC CCTGGCCTAT GACGCCTTCG TGGTCTTCGA CAAGGCGCAG 
AGCTCGGTGG CGGACTGGGT GTACAATGAG CTGCGGGTAC AGCTAGAGGA GCGCCGTGGG 
CGCCGGGCGC TACGCCTGTG TCTGGAGGAA CGTGACTGGG TACCCGGCAA AACCCTCTTT 
GAGAACCTCT GGGCCTCAGT TTACAGCAGC CGCAAGACGC TGTTTGTGCT GGCCCGCACG 
GACAGAGTCA GCGGCCTCCT GCGTGCCAGC TTCCTGCTGG CCCAACAGCG CCTGCTGGAG 
GACCGCAAGG ACGTCGTGGT GCTGGTGATC CTGTGCCCCG ACGCCCACCG CTCCCGCTAT 
GTGCGGCTGC GCCAGCGCCT CTGCCGCCAG AGTGTCCTCC TCTGGCCCCA CCAGCCCAGT 
GGCCAGCGCA GCTTCTGGGC CCAGCTGGGC ACGGCCCTGA CCAGGGACAA CCGCCACTTC 
TACAACCAGA ACTTCTGCCG GGGCCCCACG ACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977197.1
CDS	180..3278
Translation	MGPCRGALHPLSLLVQAAALALALAQGTLPAFLPCELQPHGLVNCNWLFLKSVPRFSAAAPRGNVTSLSLYSNRIHHLHDYDFVHFVHLRRLNLKWNCPPASLSPMHFPCHMTIEPNTFLAVPTLEDLNLSYNSITTVPALPSSLVSLSLSRTNILVLDPATLAGLYALRFLFLDGNCYYKNPCQQALQVAPGALLGLGNLTHLSLKYNNLTVVPRGLPPSLEYLLLSYNHIITLAPEDLANLTALRVLDVGGNCRRCDHARNPCRECPKGFPQLHPNTFGHLSHLEGLVLRDSSLYSLDPRWFHGLGNLMVLDLSENFLYDCITKTKAFYGLARLRRLNLSFNYHKKVSFAHLHLASSFGSLLSLQELDIHGIFFRSLSKTTLQSLAHLPMLQRLHLQLNFISQAQLSIFGAFPGLRYVDLSDNRISGAAEPAAATGEVEADCGERVWPQSRDLALGPLGTPGSEAFMPSCRTLNFTLDLSRNNLVTVQPEMFVRLARLQCLGLSHNSISQAVNGSQFVPLSNLRVLDLSHNKLDLYHGRSFTELPRLEALDLSYNSQPFSMRGVGHNLSFVAQLPALRYLSLAHNGIHSRVSQQLRSASLRALDFSGNTLSQMWAEGDLYLRFFQGLRSLVQLDLSQNRLHTLLPRNLDNLPKSLRLLRLRDNYLAFFNWSSLALLPKLEALDLAGNQLKALSNGSLPNGTQLQRLDLSGNSIGFVVPSFFALAVRLRELNLSANALKTVEPSWFGSLAGALKVLDVTANPLHCACGATFVDFLLEVQAAVPGLPSRVKCGSPGQLQGRSIFAQDLRLCLDEALSWVCFSLSLLAVALSLAVPMLHQLCGWDLWYCFHLCLAWLPRRGRRRGVDALAYDAFVVFDKAQSSVADWVYNELRVQLEERRGRRALRLCLEERDWVPGKTLFENLWASVYSSRKTLFVLARTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILCPDAHRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGTALTRDNRHFYNQNFCRGPTTA

Target ORF information:

RefSeq Version	XM_014121722.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 9 (TLR9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121722.2

ORF Insert Sequence:

ATGGGCCCCT GCCGTGGCGC CCTGCACCCC CTGTCTCTCC TGGTGCAGGC TGCCGCGCTA 
GCCCTGGCCC TGGCCCAGGG CACCCTGCCT GCCTTCCTGC CCTGTGAGCT CCAGCCCCAT 
GGCCTGGTGA ACTGCAACTG GCTGTTCCTC AAGTCCGTGC CCCGCTTCTC GGCAGCTGCA 
CCCCGCGGTA ACGTCACCAG CCTTTCCTTG TACTCCAACC GCATCCACCA CCTCCATGAC 
TATGACTTTG TCCACTTCGT CCACCTGCGG CGTCTCAATC TCAAGTGGAA CTGCCCGCCC 
GCCAGCCTCA GCCCCATGCA CTTTCCCTGT CACATGACCA TTGAGCCCAA CACCTTCCTG 
GCTGTGCCCA CCCTAGAGGA CCTGAATCTG AGCTATAACA GCATCACGAC TGTGCCCGCC 
CTGCCCAGTT CGCTTGTGTC CCTGTCCCTG AGCCGCACCA ACATCCTGGT GCTGGACCCT 
GCCACCCTGG CAGGCCTTTA TGCCCTGCGC TTCCTGTTCC TGGATGGCAA CTGCTACTAC 
AAGAACCCCT GCCAGCAGGC CCTGCAGGTG GCCCCAGGTG CCCTCCTGGG CCTGGGCAAC 
CTCACACACC TGTCACTCAA GTACAACAAC CTCACCGTGG TGCCGCGGGG CCTGCCCCCC 
AGCCTGGAGT ACCTGCTCTT GTCCTACAAC CACATCATCA CCCTGGCACC TGAGGACCTG 
GCCAATCTGA CTGCCCTGCG TGTCCTCGAT GTGGGTGGGA ACTGTCGCCG CTGTGACCAT 
GCCCGTAACC CCTGCAGGGA GTGCCCCAAG GGCTTCCCCC AGCTGCACCC CAACACCTTC 
GGCCACCTGA GCCACCTCGA AGGCCTGGTG TTGAGGGACA GCTCTCTCTA CAGCCTGGAC 
CCCAGGTGGT TCCATGGCCT GGGCAACCTC ATGGTGCTGG ACCTGAGTGA GAACTTCCTG 
TATGACTGCA TCACCAAAAC CAAAGCCTTC TACGGCCTGG CCCGGCTGCG CAGACTCAAC 
CTGTCCTTCA ATTATCATAA GAAGGTGTCC TTTGCCCACC TGCATCTGGC ATCCTCCTTC 
GGGAGCCTAC TGTCCCTGCA GGAGCTGGAC ATACATGGCA TCTTCTTCCG CTCGCTCAGC 
AAGACCACGC TCCAGTCGCT GGCCCACCTG CCCATGCTCC AGCGTCTGCA TCTGCAGTTG 
AACTTTATCA GCCAGGCCCA GCTCAGCATC TTCGGCGCCT TCCCTGGACT GCGGTACGTG 
GACTTGTCAG ACAACCGCAT CAGTGGAGCT GCAGAGCCCG CGGCTGCCAC AGGGGAGGTA 
GAGGCAGACT GTGGGGAGAG AGTCTGGCCA CAGTCCCGGG ACCTTGCTCT GGGCCCACTG 
GGCACCCCCG GCTCAGAGGC CTTCATGCCG AGCTGCAGGA CCCTCAACTT CACCTTGGAC 
CTGTCTCGGA ACAACCTAGT GACTGTTCAG CCGGAGATGT TTGTCCGGCT GGCGCGCCTC 
CAGTGCCTGG GCCTGAGCCA CAACAGCATC TCGCAGGCGG TCAATGGCTC GCAGTTCGTG 
CCTCTGAGCA ACCTGCGGGT GCTGGACCTG TCCCATAACA AGCTGGACCT GTACCACGGG 
CGCTCGTTCA CGGAGCTGCC GCGGCTGGAG GCCTTGGACC TCAGCTACAA CAGCCAGCCC 
TTCAGCATGC GGGGCGTGGG CCACAATCTC AGCTTTGTGG CACAGCTGCC AGCCCTGCGC 
TACCTCAGCC TGGCGCACAA TGGCATCCAC AGCCGCGTGT CCCAGCAGCT CCGCAGCGCC 
TCGCTCCGGG CCCTGGACTT CAGTGGCAAT ACCCTGAGCC AGATGTGGGC CGAGGGAGAC 
CTCTATCTCC GCTTCTTCCA AGGCCTGAGA AGCCTGGTTC AGCTGGACCT GTCCCAGAAT 
CGCCTGCATA CCCTCCTGCC ACGCAACCTG GACAACCTCC CCAAGAGCCT GCGGCTCCTG 
CGGCTCCGTG ACAATTACCT GGCTTTCTTC AACTGGAGCA GCCTGGCCCT CCTACCCAAG 
CTGGAAGCCC TGGACCTGGC GGGAAACCAG CTGAAGGCCC TGAGCAATGG CAGCTTGCCC 
AACGGCACCC AGCTCCAGAG GCTGGACCTC AGCGGCAACA GCATCGGCTT CGTGGTCCCC 
AGCTTTTTTG CCCTGGCCGT GAGGCTTCGA GAGCTCAACC TCAGCGCCAA CGCCCTCAAG 
ACGGTGGAGC CCTCCTGGTT TGGTTCCCTG GCGGGTGCCC TGAAAGTCCT AGACGTGACC 
GCCAACCCCT TGCATTGCGC TTGCGGCGCA ACCTTCGTGG ACTTCTTGCT GGAGGTGCAG 
GCTGCGGTGC CCGGCCTGCC TAGCCGTGTC AAGTGCGGCA GCCCGGGCCA GCTCCAGGGC 
CGCAGCATCT TCGCACAGGA CCTGCGCCTC TGCCTGGACG AAGCGCTCTC CTGGGTCTGT 
TTCAGCCTCT CGCTGCTGGC TGTGGCCCTG AGCCTGGCTG TGCCCATGCT GCACCAGCTC 
TGTGGCTGGG ACCTCTGGTA CTGCTTCCAC CTGTGCCTGG CCTGGCTGCC CCGGCGGGGG 
CGGCGGCGGG GTGTGGATGC CCTGGCCTAT GACGCCTTCG TGGTCTTCGA CAAGGCGCAG 
AGCTCGGTGG CGGACTGGGT GTACAATGAG CTGCGGGTAC AGCTAGAGGA GCGCCGTGGG 
CGCCGGGCGC TACGCCTGTG TCTGGAGGAA CGTGACTGGG TACCCGGCAA AACCCTCTTT 
GAGAACCTCT GGGCCTCAGT TTACAGCAGC CGCAAGACGC TGTTTGTGCT GGCCCGCACG 
GACAGAGTCA GCGGCCTCCT GCGTGCCAGC TTCCTGCTGG CCCAACAGCG CCTGCTGGAG 
GACCGCAAGG ACGTCGTGGT GCTGGTGATC CTGTGCCCCG ACGCCCACCG CTCCCGCTAT 
GTGCGGCTGC GCCAGCGCCT CTGCCGCCAG AGTGTCCTCC TCTGGCCCCA CCAGCCCAGT 
GGCCAGCGCA GCTTCTGGGC CCAGCTGGGC ACGGCCCTGA CCAGGGACAA CCGCCACTTC 
TACAACCAGA ACTTCTGCCG GGGCCCCACG ACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR9 ( XM_014121724.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977199.1 Canis lupus familiaris toll like receptor 9 (TLR9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe41552
Clone ID Related Accession (Same CDS sequence)	XM_014121724.2 , XM_014121724.1
Accession Version	XM_014121724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 9 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACC AGACGGGCAT CCACGCCACC GAGGAGCTGA AGGAGTTCTT CGCCAAGGCA 
CGGGCTGGCT CTGTCCGGCT CATCAAAGTC GTCATCGAGG ATGAGCAGCT TGTGCTGGGT 
GCCTCACGGG AGCTGGCGGG CTGCTGGGAC CAGGACTATG ACAGGACCGT ACTGCCGCTG 
CTGGACGCCC AGGAGCCCTG TTACCTGCTC TACCGCCTGG ACTCACAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTTGC TTGGTCACCT GACAATTCTC CCGTGCGGCT GAAGATGCTG 
TATGCGGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGGG GCGGCCACAT CAAGGATGAG 
CTCTTTGGGA CTGTGAAGGA CGACCTCTCC TTTGCTGGGT ACCAGAAGCA TCTGTCGTCC 
TGTGCAGCGC CCGCCCCACT GACGTCGGCT GAGAGAGAGC TTCAGCAGAT CCGTATTAAT 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAGCACCAGA CCCTGCAGGG CCTCAACTTC 
CCACTGCAGC CGGCAGCCCA GAGGGCACTT CAGCAGCTCA AACAGAAGGC CGTCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACAATTGAGC TGGTGCACAC AGAGCCCACG 
GAAGTGGCCC AGCTGCCCTC AAGGGTTCCC CGAGATGCTG CCCGCTACCA CTTCTTCCTC 
TACAAGCATA CTCATGAGGG TGACCCCCTC GAGTCTGTGG TGTTCATCTA CTCGATGCCA 
GGGTACAAGT GCAGCATCAA GGAGCGCATG CTCTATTCCA GCTGCAAGAG TCGCCTCCTC 
GACTCTGTGG AGCAGGACTT CCAGCTGGAG ATCTCCAAGA AGGGCCCCTG CCGTGGCGCC 
CTGCACCCCC TGTCTCTCCT GGTGCAGGCT GCCGCGCTAG CCCTGGCCCT GGCCCAGGGC 
ACCCTGCCTG CCTTCCTGCC CTGTGAGCTC CAGCCCCATG GCCTGGTGAA CTGCAACTGG 
CTGTTCCTCA AGTCCGTGCC CCGCTTCTCG GCAGCTGCAC CCCGCGGTAA CGTCACCAGC 
CTTTCCTTGT ACTCCAACCG CATCCACCAC CTCCATGACT ATGACTTTGT CCACTTCGTC 
CACCTGCGGC GTCTCAATCT CAAGTGGAAC TGCCCGCCCG CCAGCCTCAG CCCCATGCAC 
TTTCCCTGTC ACATGACCAT TGAGCCCAAC ACCTTCCTGG CTGTGCCCAC CCTAGAGGAC 
CTGAATCTGA GCTATAACAG CATCACGACT GTGCCCGCCC TGCCCAGTTC GCTTGTGTCC 
CTGTCCCTGA GCCGCACCAA CATCCTGGTG CTGGACCCTG CCACCCTGGC AGGCCTTTAT 
GCCCTGCGCT TCCTGTTCCT GGATGGCAAC TGCTACTACA AGAACCCCTG CCAGCAGGCC 
CTGCAGGTGG CCCCAGGTGC CCTCCTGGGC CTGGGCAACC TCACACACCT GTCACTCAAG 
TACAACAACC TCACCGTGGT GCCGCGGGGC CTGCCCCCCA GCCTGGAGTA CCTGCTCTTG 
TCCTACAACC ACATCATCAC CCTGGCACCT GAGGACCTGG CCAATCTGAC TGCCCTGCGT 
GTCCTCGATG TGGGTGGGAA CTGTCGCCGC TGTGACCATG CCCGTAACCC CTGCAGGGAG 
TGCCCCAAGG GCTTCCCCCA GCTGCACCCC AACACCTTCG GCCACCTGAG CCACCTCGAA 
GGCCTGGTGT TGAGGGACAG CTCTCTCTAC AGCCTGGACC CCAGGTGGTT CCATGGCCTG 
GGCAACCTCA TGGTGCTGGA CCTGAGTGAG AACTTCCTGT ATGACTGCAT CACCAAAACC 
AAAGCCTTCT ACGGCCTGGC CCGGCTGCGC AGACTCAACC TGTCCTTCAA TTATCATAAG 
AAGGTGTCCT TTGCCCACCT GCATCTGGCA TCCTCCTTCG GGAGCCTACT GTCCCTGCAG 
GAGCTGGACA TACATGGCAT CTTCTTCCGC TCGCTCAGCA AGACCACGCT CCAGTCGCTG 
GCCCACCTGC CCATGCTCCA GCGTCTGCAT CTGCAGTTGA ACTTTATCAG CCAGGCCCAG 
CTCAGCATCT TCGGCGCCTT CCCTGGACTG CGGTACGTGG ACTTGTCAGA CAACCGCATC 
AGTGGAGCTG CAGAGCCCGC GGCTGCCACA GGGGAGGTAG AGGCAGACTG TGGGGAGAGA 
GTCTGGCCAC AGTCCCGGGA CCTTGCTCTG GGCCCACTGG GCACCCCCGG CTCAGAGGCC 
TTCATGCCGA GCTGCAGGAC CCTCAACTTC ACCTTGGACC TGTCTCGGAA CAACCTAGTG 
ACTGTTCAGC CGGAGATGTT TGTCCGGCTG GCGCGCCTCC AGTGCCTGGG CCTGAGCCAC 
AACAGCATCT CGCAGGCGGT CAATGGCTCG CAGTTCGTGC CTCTGAGCAA CCTGCGGGTG 
CTGGACCTGT CCCATAACAA GCTGGACCTG TACCACGGGC GCTCGTTCAC GGAGCTGCCG 
CGGCTGGAGG CCTTGGACCT CAGCTACAAC AGCCAGCCCT TCAGCATGCG GGGCGTGGGC 
CACAATCTCA GCTTTGTGGC ACAGCTGCCA GCCCTGCGCT ACCTCAGCCT GGCGCACAAT 
GGCATCCACA GCCGCGTGTC CCAGCAGCTC CGCAGCGCCT CGCTCCGGGC CCTGGACTTC 
AGTGGCAATA CCCTGAGCCA GATGTGGGCC GAGGGAGACC TCTATCTCCG CTTCTTCCAA 
GGCCTGAGAA GCCTGGTTCA GCTGGACCTG TCCCAGAATC GCCTGCATAC CCTCCTGCCA 
CGCAACCTGG ACAACCTCCC CAAGAGCCTG CGGCTCCTGC GGCTCCGTGA CAATTACCTG 
GCTTTCTTCA ACTGGAGCAG CCTGGCCCTC CTACCCAAGC TGGAAGCCCT GGACCTGGCG 
GGAAACCAGC TGAAGGCCCT GAGCAATGGC AGCTTGCCCA ACGGCACCCA GCTCCAGAGG 
CTGGACCTCA GCGGCAACAG CATCGGCTTC GTGGTCCCCA GCTTTTTTGC CCTGGCCGTG 
AGGCTTCGAG AGCTCAACCT CAGCGCCAAC GCCCTCAAGA CGGTGGAGCC CTCCTGGTTT 
GGTTCCCTGG CGGGTGCCCT GAAAGTCCTA GACGTGACCG CCAACCCCTT GCATTGCGCT 
TGCGGCGCAA CCTTCGTGGA CTTCTTGCTG GAGGTGCAGG CTGCGGTGCC CGGCCTGCCT 
AGCCGTGTCA AGTGCGGCAG CCCGGGCCAG CTCCAGGGCC GCAGCATCTT CGCACAGGAC 
CTGCGCCTCT GCCTGGACGA AGCGCTCTCC TGGGTCTGTT TCAGCCTCTC GCTGCTGGCT 
GTGGCCCTGA GCCTGGCTGT GCCCATGCTG CACCAGCTCT GTGGCTGGGA CCTCTGGTAC 
TGCTTCCACC TGTGCCTGGC CTGGCTGCCC CGGCGGGGGC GGCGGCGGGG TGTGGATGCC 
CTGGCCTATG ACGCCTTCGT GGTCTTCGAC AAGGCGCAGA GCTCGGTGGC GGACTGGGTG 
TACAATGAGC TGCGGGTACA GCTAGAGGAG CGCCGTGGGC GCCGGGCGCT ACGCCTGTGT 
CTGGAGGAAC GTGACTGGGT ACCCGGCAAA ACCCTCTTTG AGAACCTCTG GGCCTCAGTT 
TACAGCAGCC GCAAGACGCT GTTTGTGCTG GCCCGCACGG ACAGAGTCAG CGGCCTCCTG 
CGTGCCAGCT TCCTGCTGGC CCAACAGCGC CTGCTGGAGG ACCGCAAGGA CGTCGTGGTG 
CTGGTGATCC TGTGCCCCGA CGCCCACCGC TCCCGCTATG TGCGGCTGCG CCAGCGCCTC 
TGCCGCCAGA GTGTCCTCCT CTGGCCCCAC CAGCCCAGTG GCCAGCGCAG CTTCTGGGCC 
CAGCTGGGCA CGGCCCTGAC CAGGGACAAC CGCCACTTCT ACAACCAGAA CTTCTGCCGG 
GGCCCCACGA CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977199.1
CDS	144..4121
Translation	MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASRELAGCWDQDYDRTVLPLLDAQEPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLNFPLQPAAQRALQQLKQKAVNYIQLKLDLERETIELVHTEPTEVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFQLEISKKGPCRGALHPLSLLVQAAALALALAQGTLPAFLPCELQPHGLVNCNWLFLKSVPRFSAAAPRGNVTSLSLYSNRIHHLHDYDFVHFVHLRRLNLKWNCPPASLSPMHFPCHMTIEPNTFLAVPTLEDLNLSYNSITTVPALPSSLVSLSLSRTNILVLDPATLAGLYALRFLFLDGNCYYKNPCQQALQVAPGALLGLGNLTHLSLKYNNLTVVPRGLPPSLEYLLLSYNHIITLAPEDLANLTALRVLDVGGNCRRCDHARNPCRECPKGFPQLHPNTFGHLSHLEGLVLRDSSLYSLDPRWFHGLGNLMVLDLSENFLYDCITKTKAFYGLARLRRLNLSFNYHKKVSFAHLHLASSFGSLLSLQELDIHGIFFRSLSKTTLQSLAHLPMLQRLHLQLNFISQAQLSIFGAFPGLRYVDLSDNRISGAAEPAAATGEVEADCGERVWPQSRDLALGPLGTPGSEAFMPSCRTLNFTLDLSRNNLVTVQPEMFVRLARLQCLGLSHNSISQAVNGSQFVPLSNLRVLDLSHNKLDLYHGRSFTELPRLEALDLSYNSQPFSMRGVGHNLSFVAQLPALRYLSLAHNGIHSRVSQQLRSASLRALDFSGNTLSQMWAEGDLYLRFFQGLRSLVQLDLSQNRLHTLLPRNLDNLPKSLRLLRLRDNYLAFFNWSSLALLPKLEALDLAGNQLKALSNGSLPNGTQLQRLDLSGNSIGFVVPSFFALAVRLRELNLSANALKTVEPSWFGSLAGALKVLDVTANPLHCACGATFVDFLLEVQAAVPGLPSRVKCGSPGQLQGRSIFAQDLRLCLDEALSWVCFSLSLLAVALSLAVPMLHQLCGWDLWYCFHLCLAWLPRRGRRRGVDALAYDAFVVFDKAQSSVADWVYNELRVQLEERRGRRALRLCLEERDWVPGKTLFENLWASVYSSRKTLFVLARTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILCPDAHRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGTALTRDNRHFYNQNFCRGPTTA

Target ORF information:

RefSeq Version	XM_014121724.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 9 (TLR9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121724.2

ORF Insert Sequence:

ATGGCGCACC AGACGGGCAT CCACGCCACC GAGGAGCTGA AGGAGTTCTT CGCCAAGGCA 
CGGGCTGGCT CTGTCCGGCT CATCAAAGTC GTCATCGAGG ATGAGCAGCT TGTGCTGGGT 
GCCTCACGGG AGCTGGCGGG CTGCTGGGAC CAGGACTATG ACAGGACCGT ACTGCCGCTG 
CTGGACGCCC AGGAGCCCTG TTACCTGCTC TACCGCCTGG ACTCACAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTTGC TTGGTCACCT GACAATTCTC CCGTGCGGCT GAAGATGCTG 
TATGCGGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGGG GCGGCCACAT CAAGGATGAG 
CTCTTTGGGA CTGTGAAGGA CGACCTCTCC TTTGCTGGGT ACCAGAAGCA TCTGTCGTCC 
TGTGCAGCGC CCGCCCCACT GACGTCGGCT GAGAGAGAGC TTCAGCAGAT CCGTATTAAT 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAGCACCAGA CCCTGCAGGG CCTCAACTTC 
CCACTGCAGC CGGCAGCCCA GAGGGCACTT CAGCAGCTCA AACAGAAGGC CGTCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACAATTGAGC TGGTGCACAC AGAGCCCACG 
GAAGTGGCCC AGCTGCCCTC AAGGGTTCCC CGAGATGCTG CCCGCTACCA CTTCTTCCTC 
TACAAGCATA CTCATGAGGG TGACCCCCTC GAGTCTGTGG TGTTCATCTA CTCGATGCCA 
GGGTACAAGT GCAGCATCAA GGAGCGCATG CTCTATTCCA GCTGCAAGAG TCGCCTCCTC 
GACTCTGTGG AGCAGGACTT CCAGCTGGAG ATCTCCAAGA AGGGCCCCTG CCGTGGCGCC 
CTGCACCCCC TGTCTCTCCT GGTGCAGGCT GCCGCGCTAG CCCTGGCCCT GGCCCAGGGC 
ACCCTGCCTG CCTTCCTGCC CTGTGAGCTC CAGCCCCATG GCCTGGTGAA CTGCAACTGG 
CTGTTCCTCA AGTCCGTGCC CCGCTTCTCG GCAGCTGCAC CCCGCGGTAA CGTCACCAGC 
CTTTCCTTGT ACTCCAACCG CATCCACCAC CTCCATGACT ATGACTTTGT CCACTTCGTC 
CACCTGCGGC GTCTCAATCT CAAGTGGAAC TGCCCGCCCG CCAGCCTCAG CCCCATGCAC 
TTTCCCTGTC ACATGACCAT TGAGCCCAAC ACCTTCCTGG CTGTGCCCAC CCTAGAGGAC 
CTGAATCTGA GCTATAACAG CATCACGACT GTGCCCGCCC TGCCCAGTTC GCTTGTGTCC 
CTGTCCCTGA GCCGCACCAA CATCCTGGTG CTGGACCCTG CCACCCTGGC AGGCCTTTAT 
GCCCTGCGCT TCCTGTTCCT GGATGGCAAC TGCTACTACA AGAACCCCTG CCAGCAGGCC 
CTGCAGGTGG CCCCAGGTGC CCTCCTGGGC CTGGGCAACC TCACACACCT GTCACTCAAG 
TACAACAACC TCACCGTGGT GCCGCGGGGC CTGCCCCCCA GCCTGGAGTA CCTGCTCTTG 
TCCTACAACC ACATCATCAC CCTGGCACCT GAGGACCTGG CCAATCTGAC TGCCCTGCGT 
GTCCTCGATG TGGGTGGGAA CTGTCGCCGC TGTGACCATG CCCGTAACCC CTGCAGGGAG 
TGCCCCAAGG GCTTCCCCCA GCTGCACCCC AACACCTTCG GCCACCTGAG CCACCTCGAA 
GGCCTGGTGT TGAGGGACAG CTCTCTCTAC AGCCTGGACC CCAGGTGGTT CCATGGCCTG 
GGCAACCTCA TGGTGCTGGA CCTGAGTGAG AACTTCCTGT ATGACTGCAT CACCAAAACC 
AAAGCCTTCT ACGGCCTGGC CCGGCTGCGC AGACTCAACC TGTCCTTCAA TTATCATAAG 
AAGGTGTCCT TTGCCCACCT GCATCTGGCA TCCTCCTTCG GGAGCCTACT GTCCCTGCAG 
GAGCTGGACA TACATGGCAT CTTCTTCCGC TCGCTCAGCA AGACCACGCT CCAGTCGCTG 
GCCCACCTGC CCATGCTCCA GCGTCTGCAT CTGCAGTTGA ACTTTATCAG CCAGGCCCAG 
CTCAGCATCT TCGGCGCCTT CCCTGGACTG CGGTACGTGG ACTTGTCAGA CAACCGCATC 
AGTGGAGCTG CAGAGCCCGC GGCTGCCACA GGGGAGGTAG AGGCAGACTG TGGGGAGAGA 
GTCTGGCCAC AGTCCCGGGA CCTTGCTCTG GGCCCACTGG GCACCCCCGG CTCAGAGGCC 
TTCATGCCGA GCTGCAGGAC CCTCAACTTC ACCTTGGACC TGTCTCGGAA CAACCTAGTG 
ACTGTTCAGC CGGAGATGTT TGTCCGGCTG GCGCGCCTCC AGTGCCTGGG CCTGAGCCAC 
AACAGCATCT CGCAGGCGGT CAATGGCTCG CAGTTCGTGC CTCTGAGCAA CCTGCGGGTG 
CTGGACCTGT CCCATAACAA GCTGGACCTG TACCACGGGC GCTCGTTCAC GGAGCTGCCG 
CGGCTGGAGG CCTTGGACCT CAGCTACAAC AGCCAGCCCT TCAGCATGCG GGGCGTGGGC 
CACAATCTCA GCTTTGTGGC ACAGCTGCCA GCCCTGCGCT ACCTCAGCCT GGCGCACAAT 
GGCATCCACA GCCGCGTGTC CCAGCAGCTC CGCAGCGCCT CGCTCCGGGC CCTGGACTTC 
AGTGGCAATA CCCTGAGCCA GATGTGGGCC GAGGGAGACC TCTATCTCCG CTTCTTCCAA 
GGCCTGAGAA GCCTGGTTCA GCTGGACCTG TCCCAGAATC GCCTGCATAC CCTCCTGCCA 
CGCAACCTGG ACAACCTCCC CAAGAGCCTG CGGCTCCTGC GGCTCCGTGA CAATTACCTG 
GCTTTCTTCA ACTGGAGCAG CCTGGCCCTC CTACCCAAGC TGGAAGCCCT GGACCTGGCG 
GGAAACCAGC TGAAGGCCCT GAGCAATGGC AGCTTGCCCA ACGGCACCCA GCTCCAGAGG 
CTGGACCTCA GCGGCAACAG CATCGGCTTC GTGGTCCCCA GCTTTTTTGC CCTGGCCGTG 
AGGCTTCGAG AGCTCAACCT CAGCGCCAAC GCCCTCAAGA CGGTGGAGCC CTCCTGGTTT 
GGTTCCCTGG CGGGTGCCCT GAAAGTCCTA GACGTGACCG CCAACCCCTT GCATTGCGCT 
TGCGGCGCAA CCTTCGTGGA CTTCTTGCTG GAGGTGCAGG CTGCGGTGCC CGGCCTGCCT 
AGCCGTGTCA AGTGCGGCAG CCCGGGCCAG CTCCAGGGCC GCAGCATCTT CGCACAGGAC 
CTGCGCCTCT GCCTGGACGA AGCGCTCTCC TGGGTCTGTT TCAGCCTCTC GCTGCTGGCT 
GTGGCCCTGA GCCTGGCTGT GCCCATGCTG CACCAGCTCT GTGGCTGGGA CCTCTGGTAC 
TGCTTCCACC TGTGCCTGGC CTGGCTGCCC CGGCGGGGGC GGCGGCGGGG TGTGGATGCC 
CTGGCCTATG ACGCCTTCGT GGTCTTCGAC AAGGCGCAGA GCTCGGTGGC GGACTGGGTG 
TACAATGAGC TGCGGGTACA GCTAGAGGAG CGCCGTGGGC GCCGGGCGCT ACGCCTGTGT 
CTGGAGGAAC GTGACTGGGT ACCCGGCAAA ACCCTCTTTG AGAACCTCTG GGCCTCAGTT 
TACAGCAGCC GCAAGACGCT GTTTGTGCTG GCCCGCACGG ACAGAGTCAG CGGCCTCCTG 
CGTGCCAGCT TCCTGCTGGC CCAACAGCGC CTGCTGGAGG ACCGCAAGGA CGTCGTGGTG 
CTGGTGATCC TGTGCCCCGA CGCCCACCGC TCCCGCTATG TGCGGCTGCG CCAGCGCCTC 
TGCCGCCAGA GTGTCCTCCT CTGGCCCCAC CAGCCCAGTG GCCAGCGCAG CTTCTGGGCC 
CAGCTGGGCA CGGCCCTGAC CAGGGACAAC CGCCACTTCT ACAACCAGAA CTTCTGCCGG 
GGCCCCACGA CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR9 ( XM_014121724.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977199.1 Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe41552
Clone ID Related Accession (Same CDS sequence)	XM_014121724.2 , XM_014121724.1
Accession Version	XM_014121724.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	toll-like receptor 9 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACC AGACGGGCAT CCACGCCACC GAGGAGCTGA AGGAGTTCTT CGCCAAGGCA 
CGGGCTGGCT CTGTCCGGCT CATCAAAGTC GTCATCGAGG ATGAGCAGCT TGTGCTGGGT 
GCCTCACGGG AGCTGGCGGG CTGCTGGGAC CAGGACTATG ACAGGACCGT ACTGCCGCTG 
CTGGACGCCC AGGAGCCCTG TTACCTGCTC TACCGCCTGG ACTCACAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTTGC TTGGTCACCT GACAATTCTC CCGTGCGGCT GAAGATGCTG 
TATGCGGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGGG GCGGCCACAT CAAGGATGAG 
CTCTTTGGGA CTGTGAAGGA CGACCTCTCC TTTGCTGGGT ACCAGAAGCA TCTGTCGTCC 
TGTGCAGCGC CCGCCCCACT GACGTCGGCT GAGAGAGAGC TTCAGCAGAT CCGTATTAAT 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAGCACCAGA CCCTGCAGGG CCTCAACTTC 
CCACTGCAGC CGGCAGCCCA GAGGGCACTT CAGCAGCTCA AACAGAAGGC CGTCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACAATTGAGC TGGTGCACAC AGAGCCCACG 
GAAGTGGCCC AGCTGCCCTC AAGGGTTCCC CGAGATGCTG CCCGCTACCA CTTCTTCCTC 
TACAAGCATA CTCATGAGGG TGACCCCCTC GAGTCTGTGG TGTTCATCTA CTCGATGCCA 
GGGTACAAGT GCAGCATCAA GGAGCGCATG CTCTATTCCA GCTGCAAGAG TCGCCTCCTC 
GACTCTGTGG AGCAGGACTT CCAGCTGGAG ATCTCCAAGA AGGGCCCCTG CCGTGGCGCC 
CTGCACCCCC TGTCTCTCCT GGTGCAGGCT GCCGCGCTAG CCCTGGCCCT GGCCCAGGGC 
ACCCTGCCTG CCTTCCTGCC CTGTGAGCTC CAGCCCCATG GCCTGGTGAA CTGCAACTGG 
CTGTTCCTCA AGTCCGTGCC CCGCTTCTCG GCAGCTGCAC CCCGCGGTAA CGTCACCAGC 
CTTTCCTTGT ACTCCAACCG CATCCACCAC CTCCATGACT ATGACTTTGT CCACTTCGTC 
CACCTGCGGC GTCTCAATCT CAAGTGGAAC TGCCCGCCCG CCAGCCTCAG CCCCATGCAC 
TTTCCCTGTC ACATGACCAT TGAGCCCAAC ACCTTCCTGG CTGTGCCCAC CCTAGAGGAC 
CTGAATCTGA GCTATAACAG CATCACGACT GTGCCCGCCC TGCCCAGTTC GCTTGTGTCC 
CTGTCCCTGA GCCGCACCAA CATCCTGGTG CTGGACCCTG CCACCCTGGC AGGCCTTTAT 
GCCCTGCGCT TCCTGTTCCT GGATGGCAAC TGCTACTACA AGAACCCCTG CCAGCAGGCC 
CTGCAGGTGG CCCCAGGTGC CCTCCTGGGC CTGGGCAACC TCACACACCT GTCACTCAAG 
TACAACAACC TCACCGTGGT GCCGCGGGGC CTGCCCCCCA GCCTGGAGTA CCTGCTCTTG 
TCCTACAACC ACATCATCAC CCTGGCACCT GAGGACCTGG CCAATCTGAC TGCCCTGCGT 
GTCCTCGATG TGGGTGGGAA CTGTCGCCGC TGTGACCATG CCCGTAACCC CTGCAGGGAG 
TGCCCCAAGG GCTTCCCCCA GCTGCACCCC AACACCTTCG GCCACCTGAG CCACCTCGAA 
GGCCTGGTGT TGAGGGACAG CTCTCTCTAC AGCCTGGACC CCAGGTGGTT CCATGGCCTG 
GGCAACCTCA TGGTGCTGGA CCTGAGTGAG AACTTCCTGT ATGACTGCAT CACCAAAACC 
AAAGCCTTCT ACGGCCTGGC CCGGCTGCGC AGACTCAACC TGTCCTTCAA TTATCATAAG 
AAGGTGTCCT TTGCCCACCT GCATCTGGCA TCCTCCTTCG GGAGCCTACT GTCCCTGCAG 
GAGCTGGACA TACATGGCAT CTTCTTCCGC TCGCTCAGCA AGACCACGCT CCAGTCGCTG 
GCCCACCTGC CCATGCTCCA GCGTCTGCAT CTGCAGTTGA ACTTTATCAG CCAGGCCCAG 
CTCAGCATCT TCGGCGCCTT CCCTGGACTG CGGTACGTGG ACTTGTCAGA CAACCGCATC 
AGTGGAGCTG CAGAGCCCGC GGCTGCCACA GGGGAGGTAG AGGCAGACTG TGGGGAGAGA 
GTCTGGCCAC AGTCCCGGGA CCTTGCTCTG GGCCCACTGG GCACCCCCGG CTCAGAGGCC 
TTCATGCCGA GCTGCAGGAC CCTCAACTTC ACCTTGGACC TGTCTCGGAA CAACCTAGTG 
ACTGTTCAGC CGGAGATGTT TGTCCGGCTG GCGCGCCTCC AGTGCCTGGG CCTGAGCCAC 
AACAGCATCT CGCAGGCGGT CAATGGCTCG CAGTTCGTGC CTCTGAGCAA CCTGCGGGTG 
CTGGACCTGT CCCATAACAA GCTGGACCTG TACCACGGGC GCTCGTTCAC GGAGCTGCCG 
CGGCTGGAGG CCTTGGACCT CAGCTACAAC AGCCAGCCCT TCAGCATGCG GGGCGTGGGC 
CACAATCTCA GCTTTGTGGC ACAGCTGCCA GCCCTGCGCT ACCTCAGCCT GGCGCACAAT 
GGCATCCACA GCCGCGTGTC CCAGCAGCTC CGCAGCGCCT CGCTCCGGGC CCTGGACTTC 
AGTGGCAATA CCCTGAGCCA GATGTGGGCC GAGGGAGACC TCTATCTCCG CTTCTTCCAA 
GGCCTGAGAA GCCTGGTTCA GCTGGACCTG TCCCAGAATC GCCTGCATAC CCTCCTGCCA 
CGCAACCTGG ACAACCTCCC CAAGAGCCTG CGGCTCCTGC GGCTCCGTGA CAATTACCTG 
GCTTTCTTCA ACTGGAGCAG CCTGGCCCTC CTACCCAAGC TGGAAGCCCT GGACCTGGCG 
GGAAACCAGC TGAAGGCCCT GAGCAATGGC AGCTTGCCCA ACGGCACCCA GCTCCAGAGG 
CTGGACCTCA GCGGCAACAG CATCGGCTTC GTGGTCCCCA GCTTTTTTGC CCTGGCCGTG 
AGGCTTCGAG AGCTCAACCT CAGCGCCAAC GCCCTCAAGA CGGTGGAGCC CTCCTGGTTT 
GGTTCCCTGG CGGGTGCCCT GAAAGTCCTA GACGTGACCG CCAACCCCTT GCATTGCGCT 
TGCGGCGCAA CCTTCGTGGA CTTCTTGCTG GAGGTGCAGG CTGCGGTGCC CGGCCTGCCT 
AGCCGTGTCA AGTGCGGCAG CCCGGGCCAG CTCCAGGGCC GCAGCATCTT CGCACAGGAC 
CTGCGCCTCT GCCTGGACGA AGCGCTCTCC TGGGTCTGTT TCAGCCTCTC GCTGCTGGCT 
GTGGCCCTGA GCCTGGCTGT GCCCATGCTG CACCAGCTCT GTGGCTGGGA CCTCTGGTAC 
TGCTTCCACC TGTGCCTGGC CTGGCTGCCC CGGCGGGGGC GGCGGCGGGG TGTGGATGCC 
CTGGCCTATG ACGCCTTCGT GGTCTTCGAC AAGGCGCAGA GCTCGGTGGC GGACTGGGTG 
TACAATGAGC TGCGGGTACA GCTAGAGGAG CGCCGTGGGC GCCGGGCGCT ACGCCTGTGT 
CTGGAGGAAC GTGACTGGGT ACCCGGCAAA ACCCTCTTTG AGAACCTCTG GGCCTCAGTT 
TACAGCAGCC GCAAGACGCT GTTTGTGCTG GCCCGCACGG ACAGAGTCAG CGGCCTCCTG 
CGTGCCAGCT TCCTGCTGGC CCAACAGCGC CTGCTGGAGG ACCGCAAGGA CGTCGTGGTG 
CTGGTGATCC TGTGCCCCGA CGCCCACCGC TCCCGCTATG TGCGGCTGCG CCAGCGCCTC 
TGCCGCCAGA GTGTCCTCCT CTGGCCCCAC CAGCCCAGTG GCCAGCGCAG CTTCTGGGCC 
CAGCTGGGCA CGGCCCTGAC CAGGGACAAC CGCCACTTCT ACAACCAGAA CTTCTGCCGG 
GGCCCCACGA CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977199.1
CDS	205..4182
Translation	MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASRELAGCWDQDYDRTVLPLLDAQEPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLNFPLQPAAQRALQQLKQKAVNYIQLKLDLERETIELVHTEPTEVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFQLEISKKGPCRGALHPLSLLVQAAALALALAQGTLPAFLPCELQPHGLVNCNWLFLKSVPRFSAAAPRGNVTSLSLYSNRIHHLHDYDFVHFVHLRRLNLKWNCPPASLSPMHFPCHMTIEPNTFLAVPTLEDLNLSYNSITTVPALPSSLVSLSLSRTNILVLDPATLAGLYALRFLFLDGNCYYKNPCQQALQVAPGALLGLGNLTHLSLKYNNLTVVPRGLPPSLEYLLLSYNHIITLAPEDLANLTALRVLDVGGNCRRCDHARNPCRECPKGFPQLHPNTFGHLSHLEGLVLRDSSLYSLDPRWFHGLGNLMVLDLSENFLYDCITKTKAFYGLARLRRLNLSFNYHKKVSFAHLHLASSFGSLLSLQELDIHGIFFRSLSKTTLQSLAHLPMLQRLHLQLNFISQAQLSIFGAFPGLRYVDLSDNRISGAAEPAAATGEVEADCGERVWPQSRDLALGPLGTPGSEAFMPSCRTLNFTLDLSRNNLVTVQPEMFVRLARLQCLGLSHNSISQAVNGSQFVPLSNLRVLDLSHNKLDLYHGRSFTELPRLEALDLSYNSQPFSMRGVGHNLSFVAQLPALRYLSLAHNGIHSRVSQQLRSASLRALDFSGNTLSQMWAEGDLYLRFFQGLRSLVQLDLSQNRLHTLLPRNLDNLPKSLRLLRLRDNYLAFFNWSSLALLPKLEALDLAGNQLKALSNGSLPNGTQLQRLDLSGNSIGFVVPSFFALAVRLRELNLSANALKTVEPSWFGSLAGALKVLDVTANPLHCACGATFVDFLLEVQAAVPGLPSRVKCGSPGQLQGRSIFAQDLRLCLDEALSWVCFSLSLLAVALSLAVPMLHQLCGWDLWYCFHLCLAWLPRRGRRRGVDALAYDAFVVFDKAQSSVADWVYNELRVQLEERRGRRALRLCLEERDWVPGKTLFENLWASVYSSRKTLFVLARTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILCPDAHRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGTALTRDNRHFYNQNFCRGPTTA

Target ORF information:

RefSeq Version	XM_014121724.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris toll-like receptor 9 (TLR9), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121724.1

ORF Insert Sequence:

ATGGCGCACC AGACGGGCAT CCACGCCACC GAGGAGCTGA AGGAGTTCTT CGCCAAGGCA 
CGGGCTGGCT CTGTCCGGCT CATCAAAGTC GTCATCGAGG ATGAGCAGCT TGTGCTGGGT 
GCCTCACGGG AGCTGGCGGG CTGCTGGGAC CAGGACTATG ACAGGACCGT ACTGCCGCTG 
CTGGACGCCC AGGAGCCCTG TTACCTGCTC TACCGCCTGG ACTCACAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTTGC TTGGTCACCT GACAATTCTC CCGTGCGGCT GAAGATGCTG 
TATGCGGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGGG GCGGCCACAT CAAGGATGAG 
CTCTTTGGGA CTGTGAAGGA CGACCTCTCC TTTGCTGGGT ACCAGAAGCA TCTGTCGTCC 
TGTGCAGCGC CCGCCCCACT GACGTCGGCT GAGAGAGAGC TTCAGCAGAT CCGTATTAAT 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAGCACCAGA CCCTGCAGGG CCTCAACTTC 
CCACTGCAGC CGGCAGCCCA GAGGGCACTT CAGCAGCTCA AACAGAAGGC CGTCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACAATTGAGC TGGTGCACAC AGAGCCCACG 
GAAGTGGCCC AGCTGCCCTC AAGGGTTCCC CGAGATGCTG CCCGCTACCA CTTCTTCCTC 
TACAAGCATA CTCATGAGGG TGACCCCCTC GAGTCTGTGG TGTTCATCTA CTCGATGCCA 
GGGTACAAGT GCAGCATCAA GGAGCGCATG CTCTATTCCA GCTGCAAGAG TCGCCTCCTC 
GACTCTGTGG AGCAGGACTT CCAGCTGGAG ATCTCCAAGA AGGGCCCCTG CCGTGGCGCC 
CTGCACCCCC TGTCTCTCCT GGTGCAGGCT GCCGCGCTAG CCCTGGCCCT GGCCCAGGGC 
ACCCTGCCTG CCTTCCTGCC CTGTGAGCTC CAGCCCCATG GCCTGGTGAA CTGCAACTGG 
CTGTTCCTCA AGTCCGTGCC CCGCTTCTCG GCAGCTGCAC CCCGCGGTAA CGTCACCAGC 
CTTTCCTTGT ACTCCAACCG CATCCACCAC CTCCATGACT ATGACTTTGT CCACTTCGTC 
CACCTGCGGC GTCTCAATCT CAAGTGGAAC TGCCCGCCCG CCAGCCTCAG CCCCATGCAC 
TTTCCCTGTC ACATGACCAT TGAGCCCAAC ACCTTCCTGG CTGTGCCCAC CCTAGAGGAC 
CTGAATCTGA GCTATAACAG CATCACGACT GTGCCCGCCC TGCCCAGTTC GCTTGTGTCC 
CTGTCCCTGA GCCGCACCAA CATCCTGGTG CTGGACCCTG CCACCCTGGC AGGCCTTTAT 
GCCCTGCGCT TCCTGTTCCT GGATGGCAAC TGCTACTACA AGAACCCCTG CCAGCAGGCC 
CTGCAGGTGG CCCCAGGTGC CCTCCTGGGC CTGGGCAACC TCACACACCT GTCACTCAAG 
TACAACAACC TCACCGTGGT GCCGCGGGGC CTGCCCCCCA GCCTGGAGTA CCTGCTCTTG 
TCCTACAACC ACATCATCAC CCTGGCACCT GAGGACCTGG CCAATCTGAC TGCCCTGCGT 
GTCCTCGATG TGGGTGGGAA CTGTCGCCGC TGTGACCATG CCCGTAACCC CTGCAGGGAG 
TGCCCCAAGG GCTTCCCCCA GCTGCACCCC AACACCTTCG GCCACCTGAG CCACCTCGAA 
GGCCTGGTGT TGAGGGACAG CTCTCTCTAC AGCCTGGACC CCAGGTGGTT CCATGGCCTG 
GGCAACCTCA TGGTGCTGGA CCTGAGTGAG AACTTCCTGT ATGACTGCAT CACCAAAACC 
AAAGCCTTCT ACGGCCTGGC CCGGCTGCGC AGACTCAACC TGTCCTTCAA TTATCATAAG 
AAGGTGTCCT TTGCCCACCT GCATCTGGCA TCCTCCTTCG GGAGCCTACT GTCCCTGCAG 
GAGCTGGACA TACATGGCAT CTTCTTCCGC TCGCTCAGCA AGACCACGCT CCAGTCGCTG 
GCCCACCTGC CCATGCTCCA GCGTCTGCAT CTGCAGTTGA ACTTTATCAG CCAGGCCCAG 
CTCAGCATCT TCGGCGCCTT CCCTGGACTG CGGTACGTGG ACTTGTCAGA CAACCGCATC 
AGTGGAGCTG CAGAGCCCGC GGCTGCCACA GGGGAGGTAG AGGCAGACTG TGGGGAGAGA 
GTCTGGCCAC AGTCCCGGGA CCTTGCTCTG GGCCCACTGG GCACCCCCGG CTCAGAGGCC 
TTCATGCCGA GCTGCAGGAC CCTCAACTTC ACCTTGGACC TGTCTCGGAA CAACCTAGTG 
ACTGTTCAGC CGGAGATGTT TGTCCGGCTG GCGCGCCTCC AGTGCCTGGG CCTGAGCCAC 
AACAGCATCT CGCAGGCGGT CAATGGCTCG CAGTTCGTGC CTCTGAGCAA CCTGCGGGTG 
CTGGACCTGT CCCATAACAA GCTGGACCTG TACCACGGGC GCTCGTTCAC GGAGCTGCCG 
CGGCTGGAGG CCTTGGACCT CAGCTACAAC AGCCAGCCCT TCAGCATGCG GGGCGTGGGC 
CACAATCTCA GCTTTGTGGC ACAGCTGCCA GCCCTGCGCT ACCTCAGCCT GGCGCACAAT 
GGCATCCACA GCCGCGTGTC CCAGCAGCTC CGCAGCGCCT CGCTCCGGGC CCTGGACTTC 
AGTGGCAATA CCCTGAGCCA GATGTGGGCC GAGGGAGACC TCTATCTCCG CTTCTTCCAA 
GGCCTGAGAA GCCTGGTTCA GCTGGACCTG TCCCAGAATC GCCTGCATAC CCTCCTGCCA 
CGCAACCTGG ACAACCTCCC CAAGAGCCTG CGGCTCCTGC GGCTCCGTGA CAATTACCTG 
GCTTTCTTCA ACTGGAGCAG CCTGGCCCTC CTACCCAAGC TGGAAGCCCT GGACCTGGCG 
GGAAACCAGC TGAAGGCCCT GAGCAATGGC AGCTTGCCCA ACGGCACCCA GCTCCAGAGG 
CTGGACCTCA GCGGCAACAG CATCGGCTTC GTGGTCCCCA GCTTTTTTGC CCTGGCCGTG 
AGGCTTCGAG AGCTCAACCT CAGCGCCAAC GCCCTCAAGA CGGTGGAGCC CTCCTGGTTT 
GGTTCCCTGG CGGGTGCCCT GAAAGTCCTA GACGTGACCG CCAACCCCTT GCATTGCGCT 
TGCGGCGCAA CCTTCGTGGA CTTCTTGCTG GAGGTGCAGG CTGCGGTGCC CGGCCTGCCT 
AGCCGTGTCA AGTGCGGCAG CCCGGGCCAG CTCCAGGGCC GCAGCATCTT CGCACAGGAC 
CTGCGCCTCT GCCTGGACGA AGCGCTCTCC TGGGTCTGTT TCAGCCTCTC GCTGCTGGCT 
GTGGCCCTGA GCCTGGCTGT GCCCATGCTG CACCAGCTCT GTGGCTGGGA CCTCTGGTAC 
TGCTTCCACC TGTGCCTGGC CTGGCTGCCC CGGCGGGGGC GGCGGCGGGG TGTGGATGCC 
CTGGCCTATG ACGCCTTCGT GGTCTTCGAC AAGGCGCAGA GCTCGGTGGC GGACTGGGTG 
TACAATGAGC TGCGGGTACA GCTAGAGGAG CGCCGTGGGC GCCGGGCGCT ACGCCTGTGT 
CTGGAGGAAC GTGACTGGGT ACCCGGCAAA ACCCTCTTTG AGAACCTCTG GGCCTCAGTT 
TACAGCAGCC GCAAGACGCT GTTTGTGCTG GCCCGCACGG ACAGAGTCAG CGGCCTCCTG 
CGTGCCAGCT TCCTGCTGGC CCAACAGCGC CTGCTGGAGG ACCGCAAGGA CGTCGTGGTG 
CTGGTGATCC TGTGCCCCGA CGCCCACCGC TCCCGCTATG TGCGGCTGCG CCAGCGCCTC 
TGCCGCCAGA GTGTCCTCCT CTGGCCCCAC CAGCCCAGTG GCCAGCGCAG CTTCTGGGCC 
CAGCTGGGCA CGGCCCTGAC CAGGGACAAC CGCCACTTCT ACAACCAGAA CTTCTGCCGG 
GGCCCCACGA CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.
Parasite immunology36(12)726-31(2014 Dec)
Melo GD,Silva JE,Grano FG,Homem CG,Machado GF

Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.
BMC veterinary research10187(2014 Aug)
Mercier E,Peters IR,Farnir F,Lavou? R,Day M,Clercx C,Peeters D

Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
BMC immunology1422(2013 May)
Figueiredo MM,Amorim IF,Pinto AJ,Barbosa VS,Pinheiro Lde J,Deoti B,Faria AM,Tafuri WL

Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle.
International journal of experimental pathology92(1)1-7(2011 Feb)
Schneberger D,Lewis D,Caldwell S,Singh B

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TLR9 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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