COL4A5 cDNA ORF clone, Canis lupus familiaris(Dog)

The following COL4A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe33995 NM_001002979.1
Latest version!
Canis lupus familiaris collagen type IV alpha 5 chain (COL4A5), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31922 XM_005640969.3
Latest version!
Canis lupus familiaris collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31923 XM_005640970.3
Latest version!
Canis lupus familiaris collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OCe31922 XM_005640969.2 Canis lupus familiaris collagen, type IV, alpha 5 (COL4A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31923 XM_005640970.2 Canis lupus familiaris collagen, type IV, alpha 5 (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe33995
    Clone ID Related Accession (Same CDS sequence) NM_001002979.1
    Accession Version NM_001002979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-5(IV) chain precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF470624.2. ##Evidence-Data-START## Transcript exon combination :: AF470624.2, AY078501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CAGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGACCAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCGGGGTCTC
    CCTGGAAATG GAGGTATTAA AGGAGAGAGA GGAAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGAATCC AGGGTAATCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGTATG
    AAGGGACCCA GTGGAGTACC AGGTTCAGCT GGCCCTGAGG GGGATCCAGG ACTTGTTGGC
    CCACCAGGTC CCCCTGGACT ACCTGGTCCT TCAGGACAGA GTATTATAAT CAAAGGAGAT
    GTTGGTCCTC CAGGGATCCC AGGCCAGCCT GGATTAAAAG GTCTACCAGG ACTACCAGGA
    CCTCAGGGCT TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA CGGACTCCCT
    GGCTTTGATG GCGCTGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGCCA GCCAGGTACC
    CGTGGTTTGG ATGGTCCCCC TGGACCAGAT GGAATGCAAG GTCCTCCAGG TCCTCCAGGA
    ACTTCCTCTA TTGCCCATGG ATTCCTCATC ACACGTCACA GCCAGACAAC AGATGCACCA
    CAATGCCCAC ATGGAACTGT CCAGATTTAT GAAGGCTTTT CTCTCTTATA TGTCCAAGGA
    AATAAAAGAG CTCACGGTCA AGACTTGGGG ACGGCTGGCA GTTGCCTTCG TCGCTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAC AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTGTCTAC CCCAGAGCCC ATGCCAATGA GCATGGAGCC CCTGAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGCG AAGCTCCAGC TGTGGTGATT
    GCAGTTCACA GTCAGACCAT CCAGATTCCC CATTGTCCTC ACGGATGGGA TTCTCTCTGG
    ATTGGCTATT CCTTCATGAT GCACACGAGC GCAGGAGCAG AGGGCTCAGG TCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CGTTCATTGA ATGTCATGGG
    CGGGGTACCT GCAACTACTA TGCCAACTCC TACAGCTTTT GGCTAGCAAC TGTAGATGTG
    TCAGATATGT TCAGCAAACC TCAGTCAGAA ACACTGAAAG CAGGAGACTT GAGAACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002979.1
    CDS177..5252
    Translation

    Target ORF information:

    RefSeq Version NM_001002979.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 5 chain (COL4A5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CAGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGACCAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCGGGGTCTC
    CCTGGAAATG GAGGTATTAA AGGAGAGAGA GGAAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGAATCC AGGGTAATCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGTATG
    AAGGGACCCA GTGGAGTACC AGGTTCAGCT GGCCCTGAGG GGGATCCAGG ACTTGTTGGC
    CCACCAGGTC CCCCTGGACT ACCTGGTCCT TCAGGACAGA GTATTATAAT CAAAGGAGAT
    GTTGGTCCTC CAGGGATCCC AGGCCAGCCT GGATTAAAAG GTCTACCAGG ACTACCAGGA
    CCTCAGGGCT TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA CGGACTCCCT
    GGCTTTGATG GCGCTGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGCCA GCCAGGTACC
    CGTGGTTTGG ATGGTCCCCC TGGACCAGAT GGAATGCAAG GTCCTCCAGG TCCTCCAGGA
    ACTTCCTCTA TTGCCCATGG ATTCCTCATC ACACGTCACA GCCAGACAAC AGATGCACCA
    CAATGCCCAC ATGGAACTGT CCAGATTTAT GAAGGCTTTT CTCTCTTATA TGTCCAAGGA
    AATAAAAGAG CTCACGGTCA AGACTTGGGG ACGGCTGGCA GTTGCCTTCG TCGCTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAC AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTGTCTAC CCCAGAGCCC ATGCCAATGA GCATGGAGCC CCTGAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGCG AAGCTCCAGC TGTGGTGATT
    GCAGTTCACA GTCAGACCAT CCAGATTCCC CATTGTCCTC ACGGATGGGA TTCTCTCTGG
    ATTGGCTATT CCTTCATGAT GCACACGAGC GCAGGAGCAG AGGGCTCAGG TCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CGTTCATTGA ATGTCATGGG
    CGGGGTACCT GCAACTACTA TGCCAACTCC TACAGCTTTT GGCTAGCAAC TGTAGATGTG
    TCAGATATGT TCAGCAAACC TCAGTCAGAA ACACTGAAAG CAGGAGACTT GAGAACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31922
    Clone ID Related Accession (Same CDS sequence) XM_005640969.2 , XM_005640969.3
    Accession Version XM_005640969.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-5(IV) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTTTTCAAGG TCTACCAGGT CCAGAAGGTC CCCGGGGTCT CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG AGGAAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGAATC CAGGGTAATC CTGGCCGGCC AGGTCTCAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGTAT GAAGGGACCC
    AGTGGAGTAC CAGGTTCAGC TGGCCCTGAG GGGGATCCAG GACTTGTTGG CCCACCAGGT
    CCCCCTGGAC TACCTGGTCC TTCAGGACAG AGTATTATAA TCAAAGGAGA TGTTGGTCCA
    CCAGGGATCC CAGGCCAGCC TGGATTAAAA GGTCTACCAG GACTACCAGG ACCTCAGGGC
    TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ACGGACTCCC TGGCTTTGAT
    GGCGCTGGAG GGCGCAAAGG AGACCCAGGT CTGCCAGGCC AGCCAGGTAC CCGTGGTTTG
    GATGGTCCCC CTGGACCAGA TGGAATGCAA GGTCCTCCAG GTCCTCCAGG AACTTCCTCT
    ATTGCCCATG GATTCCTCAT CACACGTCAC AGCCAGACAA CAGATGCACC ACAATGCCCA
    CATGGAACTG TCCAGATTTA TGAAGGCTTT TCTCTCTTAT ATGTCCAAGG AAATAAAAGA
    GCTCACGGTC AAGACTTGGG GACGGCTGGC AGTTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATCAA CAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC
    TGGCTGTCTA CCCCAGAGCC CATGCCAATG AGCATGGAGC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGC GAAGCTCCAG CTGTGGTGAT TGCAGTTCAC
    AGTCAGACCA TCCAGATTCC CCATTGTCCT CACGGATGGG ATTCTCTCTG GATTGGCTAT
    TCCTTCATGA TGCACACGAG CGCAGGAGCA GAGGGCTCAG GTCAAGCCCT GGCCTCCCCT
    GGTTCCTGCT TGGAAGAGTT TCGTTCAGCT CCTTTCATTG AATGTCATGG GCGGGGTACC
    TGCAACTACT ATGCCAACTC CTACAGCTTT TGGCTAGCAA CTGTAGATGT GTCAGATATG
    TTCAGCAAAC CTCAGTCAGA AACACTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTGT GCATGAAGAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641026.1
    CDS314..5380
    Translation

    Target ORF information:

    RefSeq Version XM_005640969.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 5 (COL4A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640969.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTTTTCAAGG TCTACCAGGT CCAGAAGGTC CCCGGGGTCT CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG AGGAAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGAATC CAGGGTAATC CTGGCCGGCC AGGTCTCAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGTAT GAAGGGACCC
    AGTGGAGTAC CAGGTTCAGC TGGCCCTGAG GGGGATCCAG GACTTGTTGG CCCACCAGGT
    CCCCCTGGAC TACCTGGTCC TTCAGGACAG AGTATTATAA TCAAAGGAGA TGTTGGTCCA
    CCAGGGATCC CAGGCCAGCC TGGATTAAAA GGTCTACCAG GACTACCAGG ACCTCAGGGC
    TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ACGGACTCCC TGGCTTTGAT
    GGCGCTGGAG GGCGCAAAGG AGACCCAGGT CTGCCAGGCC AGCCAGGTAC CCGTGGTTTG
    GATGGTCCCC CTGGACCAGA TGGAATGCAA GGTCCTCCAG GTCCTCCAGG AACTTCCTCT
    ATTGCCCATG GATTCCTCAT CACACGTCAC AGCCAGACAA CAGATGCACC ACAATGCCCA
    CATGGAACTG TCCAGATTTA TGAAGGCTTT TCTCTCTTAT ATGTCCAAGG AAATAAAAGA
    GCTCACGGTC AAGACTTGGG GACGGCTGGC AGTTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATCAA CAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC
    TGGCTGTCTA CCCCAGAGCC CATGCCAATG AGCATGGAGC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGC GAAGCTCCAG CTGTGGTGAT TGCAGTTCAC
    AGTCAGACCA TCCAGATTCC CCATTGTCCT CACGGATGGG ATTCTCTCTG GATTGGCTAT
    TCCTTCATGA TGCACACGAG CGCAGGAGCA GAGGGCTCAG GTCAAGCCCT GGCCTCCCCT
    GGTTCCTGCT TGGAAGAGTT TCGTTCAGCT CCTTTCATTG AATGTCATGG GCGGGGTACC
    TGCAACTACT ATGCCAACTC CTACAGCTTT TGGCTAGCAA CTGTAGATGT GTCAGATATG
    TTCAGCAAAC CTCAGTCAGA AACACTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTGT GCATGAAGAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31922
    Clone ID Related Accession (Same CDS sequence) XM_005640969.2 , XM_005640969.3
    Accession Version XM_005640969.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTTTTCAAGG TCTACCAGGT CCAGAAGGTC CCCGGGGTCT CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG AGGAAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGAATC CAGGGTAATC CTGGCCGGCC AGGTCTCAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGTAT GAAGGGACCC
    AGTGGAGTAC CAGGTTCAGC TGGCCCTGAG GGGGATCCAG GACTTGTTGG CCCACCAGGT
    CCCCCTGGAC TACCTGGTCC TTCAGGACAG AGTATTATAA TCAAAGGAGA TGTTGGTCCA
    CCAGGGATCC CAGGCCAGCC TGGATTAAAA GGTCTACCAG GACTACCAGG ACCTCAGGGC
    TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ACGGACTCCC TGGCTTTGAT
    GGCGCTGGAG GGCGCAAAGG AGACCCAGGT CTGCCAGGCC AGCCAGGTAC CCGTGGTTTG
    GATGGTCCCC CTGGACCAGA TGGAATGCAA GGTCCTCCAG GTCCTCCAGG AACTTCCTCT
    ATTGCCCATG GATTCCTCAT CACACGTCAC AGCCAGACAA CAGATGCACC ACAATGCCCA
    CATGGAACTG TCCAGATTTA TGAAGGCTTT TCTCTCTTAT ATGTCCAAGG AAATAAAAGA
    GCTCACGGTC AAGACTTGGG GACGGCTGGC AGTTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATCAA CAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC
    TGGCTGTCTA CCCCAGAGCC CATGCCAATG AGCATGGAGC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGC GAAGCTCCAG CTGTGGTGAT TGCAGTTCAC
    AGTCAGACCA TCCAGATTCC CCATTGTCCT CACGGATGGG ATTCTCTCTG GATTGGCTAT
    TCCTTCATGA TGCACACGAG CGCAGGAGCA GAGGGCTCAG GTCAAGCCCT GGCCTCCCCT
    GGTTCCTGCT TGGAAGAGTT TCGTTCAGCT CCTTTCATTG AATGTCATGG GCGGGGTACC
    TGCAACTACT ATGCCAACTC CTACAGCTTT TGGCTAGCAA CTGTAGATGT GTCAGATATG
    TTCAGCAAAC CTCAGTCAGA AACACTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTGT GCATGAAGAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641026.1
    CDS331..5397
    Translation

    Target ORF information:

    RefSeq Version XM_005640969.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640969.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTTTTCAAGG TCTACCAGGT CCAGAAGGTC CCCGGGGTCT CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG AGGAAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGAATC CAGGGTAATC CTGGCCGGCC AGGTCTCAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGTAT GAAGGGACCC
    AGTGGAGTAC CAGGTTCAGC TGGCCCTGAG GGGGATCCAG GACTTGTTGG CCCACCAGGT
    CCCCCTGGAC TACCTGGTCC TTCAGGACAG AGTATTATAA TCAAAGGAGA TGTTGGTCCA
    CCAGGGATCC CAGGCCAGCC TGGATTAAAA GGTCTACCAG GACTACCAGG ACCTCAGGGC
    TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ACGGACTCCC TGGCTTTGAT
    GGCGCTGGAG GGCGCAAAGG AGACCCAGGT CTGCCAGGCC AGCCAGGTAC CCGTGGTTTG
    GATGGTCCCC CTGGACCAGA TGGAATGCAA GGTCCTCCAG GTCCTCCAGG AACTTCCTCT
    ATTGCCCATG GATTCCTCAT CACACGTCAC AGCCAGACAA CAGATGCACC ACAATGCCCA
    CATGGAACTG TCCAGATTTA TGAAGGCTTT TCTCTCTTAT ATGTCCAAGG AAATAAAAGA
    GCTCACGGTC AAGACTTGGG GACGGCTGGC AGTTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATCAA CAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC
    TGGCTGTCTA CCCCAGAGCC CATGCCAATG AGCATGGAGC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGC GAAGCTCCAG CTGTGGTGAT TGCAGTTCAC
    AGTCAGACCA TCCAGATTCC CCATTGTCCT CACGGATGGG ATTCTCTCTG GATTGGCTAT
    TCCTTCATGA TGCACACGAG CGCAGGAGCA GAGGGCTCAG GTCAAGCCCT GGCCTCCCCT
    GGTTCCTGCT TGGAAGAGTT TCGTTCAGCT CCTTTCATTG AATGTCATGG GCGGGGTACC
    TGCAACTACT ATGCCAACTC CTACAGCTTT TGGCTAGCAA CTGTAGATGT GTCAGATATG
    TTCAGCAAAC CTCAGTCAGA AACACTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTGT GCATGAAGAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31923
    Clone ID Related Accession (Same CDS sequence) XM_005640970.3 , XM_005640970.2
    Accession Version XM_005640970.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCCCGGGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GAGGAAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGAAT CCAGGGTAAT CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGTA TGAAGGGACC CAGTGGAGTA
    CCAGGTTCAG CTGGCCCTGA GGGGGATCCA GGACTTGTTG GCCCACCAGG TCCCCCTGGA
    CTACCTGGTC CTTCAGGACA GAGTATTATA ATCAAAGGAG ATGTTGGTCC ACCAGGGATC
    CCAGGCCAGC CTGGATTAAA AGGTCTACCA GGACTACCAG GACCTCAGGG CTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AACGGACTCC CTGGCTTTGA TGGCGCTGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGC CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGACCAG ATGGAATGCA AGGTCCTCCA GGTCCTCCAG GAACTTCCTC TATTGCCCAT
    GGATTCCTCA TCACACGTCA CAGCCAGACA ACAGATGCAC CACAATGCCC ACATGGAACT
    GTCCAGATTT ATGAAGGCTT TTCTCTCTTA TATGTCCAAG GAAATAAAAG AGCTCACGGT
    CAAGACTTGG GGACGGCTGG CAGTTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ACAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTGTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGGAG CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG CGAAGCTCCA GCTGTGGTGA TTGCAGTTCA CAGTCAGACC
    ATCCAGATTC CCCATTGTCC TCACGGATGG GATTCTCTCT GGATTGGCTA TTCCTTCATG
    ATGCACACGA GCGCAGGAGC AGAGGGCTCA GGTCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCTTTCATT GAATGTCATG GGCGGGGTAC CTGCAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTAGCA ACTGTAGATG TGTCAGATAT GTTCAGCAAA
    CCTCAGTCAG AAACACTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641027.1
    CDS331..5388
    Translation

    Target ORF information:

    RefSeq Version XM_005640970.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640970.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCCCGGGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GAGGAAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGAAT CCAGGGTAAT CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGTA TGAAGGGACC CAGTGGAGTA
    CCAGGTTCAG CTGGCCCTGA GGGGGATCCA GGACTTGTTG GCCCACCAGG TCCCCCTGGA
    CTACCTGGTC CTTCAGGACA GAGTATTATA ATCAAAGGAG ATGTTGGTCC ACCAGGGATC
    CCAGGCCAGC CTGGATTAAA AGGTCTACCA GGACTACCAG GACCTCAGGG CTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AACGGACTCC CTGGCTTTGA TGGCGCTGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGC CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGACCAG ATGGAATGCA AGGTCCTCCA GGTCCTCCAG GAACTTCCTC TATTGCCCAT
    GGATTCCTCA TCACACGTCA CAGCCAGACA ACAGATGCAC CACAATGCCC ACATGGAACT
    GTCCAGATTT ATGAAGGCTT TTCTCTCTTA TATGTCCAAG GAAATAAAAG AGCTCACGGT
    CAAGACTTGG GGACGGCTGG CAGTTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ACAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTGTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGGAG CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG CGAAGCTCCA GCTGTGGTGA TTGCAGTTCA CAGTCAGACC
    ATCCAGATTC CCCATTGTCC TCACGGATGG GATTCTCTCT GGATTGGCTA TTCCTTCATG
    ATGCACACGA GCGCAGGAGC AGAGGGCTCA GGTCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCTTTCATT GAATGTCATG GGCGGGGTAC CTGCAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTAGCA ACTGTAGATG TGTCAGATAT GTTCAGCAAA
    CCTCAGTCAG AAACACTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31923
    Clone ID Related Accession (Same CDS sequence) XM_005640970.3 , XM_005640970.2
    Accession Version XM_005640970.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-5(IV) chain isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCCCGGGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GAGGAAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGAAT CCAGGGTAAT CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGTA TGAAGGGACC CAGTGGAGTA
    CCAGGTTCAG CTGGCCCTGA GGGGGATCCA GGACTTGTTG GCCCACCAGG TCCCCCTGGA
    CTACCTGGTC CTTCAGGACA GAGTATTATA ATCAAAGGAG ATGTTGGTCC ACCAGGGATC
    CCAGGCCAGC CTGGATTAAA AGGTCTACCA GGACTACCAG GACCTCAGGG CTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AACGGACTCC CTGGCTTTGA TGGCGCTGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGC CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGACCAG ATGGAATGCA AGGTCCTCCA GGTCCTCCAG GAACTTCCTC TATTGCCCAT
    GGATTCCTCA TCACACGTCA CAGCCAGACA ACAGATGCAC CACAATGCCC ACATGGAACT
    GTCCAGATTT ATGAAGGCTT TTCTCTCTTA TATGTCCAAG GAAATAAAAG AGCTCACGGT
    CAAGACTTGG GGACGGCTGG CAGTTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ACAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTGTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGGAG CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG CGAAGCTCCA GCTGTGGTGA TTGCAGTTCA CAGTCAGACC
    ATCCAGATTC CCCATTGTCC TCACGGATGG GATTCTCTCT GGATTGGCTA TTCCTTCATG
    ATGCACACGA GCGCAGGAGC AGAGGGCTCA GGTCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCTTTCATT GAATGTCATG GGCGGGGTAC CTGCAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTAGCA ACTGTAGATG TGTCAGATAT GTTCAGCAAA
    CCTCAGTCAG AAACACTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641027.1
    CDS314..5371
    Translation

    Target ORF information:

    RefSeq Version XM_005640970.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 5 (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640970.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA ATGTGACTGT
    AGTGGTGTAA AAGGAGAAAA GGGGGAGAGA GGATTTCCGG GTTTGGAAGG CCATCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCGAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CGGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC TGGTTTTCCT
    GGTCTAGAGG GTCCTCCGGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC CAGCTGGTCC CACTGGTTTA CCAGGGCCAA TTGGTCCCCC AGGACCACCT
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGTCC CCCTGGTCCT
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTA TGAAAGGTGA GAAGGGTGAA CAAGGAGAGC CTGGCAAAAG AGGTAAACCG
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TACCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGGA TGGAGAAAAG GGCCAAAAAG GTGACATTGG CTCAACTGGG
    CCTCCTGGAC TTGTAATTCC TAGGCCTGGG ACCGGTGTAA CTGTAGGAGA GAAAGGAAAC
    ATGGGGTTAC CTGGCTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGACT GCAGTTATGG GTCCTCCTGG CCCCCCTGGC
    TTTCCTGGAG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATATC TATTCCTGGA
    TTTCCCGGAC TTGATGGGCA GCCTGGGGCT CCTGGACTTC GAGGGCCTCC TGGCCCTCCT
    GGCCCTCACA TCTCTCCTAG TGATGAGATA TGTGAAACAG GCCCTCCAGG CCCTCCCGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGATACC
    TGCTTCAACT GTATTGGAAC TGGCGTTTCA GGGCCTCGAG GTCAACCTGG TCTCCCAGGT
    CTTCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGAGAAAA AGGACATGCT
    GGTCTAACAG GTCCCAAAGG ATTAACAGGC ATACCGGGAG CTCCAGGTCC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGACGTC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTACCCTGG AGCCCCTGGG CTTCCTGGTT TACCTGGAAC CCCTGGACAG
    GATGGACTGC CAGGGCTTCC TGGCCCCAAA GGAGAACCTG GTGGAATTGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA TCCAGGCTTG CCAGGTCTCC CAGGGAATAG GGGGCCTATG
    GGCCCTGTGG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCGGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAGCCAGGGA AGCCTGGCCT GCCTGGTAAT CCAGGCAGAC ATGGTGAAGT AGGGCTTCCG
    GGTGACCCTG GACTTCCCGG CCCGCCGGGC TTGCCAGGAA TACCCGGTAA TAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CAAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGACTTG GGCTGCCTGG GCCACCTGGA
    CCCCCAGGAC TCCCAGGTTT CAAAGGAACA CTTGGTCCAA AAGGAGATCG TGGTTTCCCA
    GGACCTCCAG GTCTTCCCGG ACGTACTGGC TTGGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGGCCAA AAGGACAACC TGGGACAATG GGACCTCCTG GGCTTCCAGG AATAGGTGTT
    CAGGGACCAC CAGGGCCACC AGGGATTCCT GGACCAGTAG GTGAACCTGG CCTACACGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGGCCT CCTGGGTTTG ACGTTCTAGG ACCCCCTGGT
    GAGAGAGGCA GTCCAGGGAT TCCTGGAGCA CCTGGTCCTA TGGGACCCCC AGGAACACCA
    GGGCTTCCAG GAAAAGCAGG AGCCTCTGGA TTTCCAGGTG CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CCGGCCCTCC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TATTCCTGGT
    CTAAAAGGTG ATAATGGTTT GCAGGGTCAG CCAGGACCTC CTGGCCCTGA AGGAGAAAAA
    GGTGGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAGTGGATCC AGATCTGTTG
    GGCTCAAAAG GAGAGAAGGG GGACCCTGGC TTACCAGGTA TTCCTGGAGT TTCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CATCAGGTCC CAAAGGTAAC CCCGGCCTCC CTGGGAAACC AGGGCTTACA
    GGACCTCCTG GGCTTAAAGG AAGCATAGGT GATATGGGTT TTCCAGGTCC TCAGGGTGTG
    AAAGGGTCTC CTGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAAAAAGGTG AAAAAGGTGA TCCTGGTGTT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    CCAAAGGGTG AAGCTGGTCT TCCTGGATAC CCAGGAAACC CTGGAATCAA AGGTTCTATG
    GGAGATACTG GTTTGCCTGG ATTACCAGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CCGGAACCCC AGGACTTCCT GGACCAAAAG GTATTAATGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCC GTAGGTGGTG GAGGGCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG AAACCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCTGGT
    CCAAAAGGAG AACCAGGCTT TCAGGGTTTC CCTGGTGTCC AGGGTCCCCC AGGCCCTCCT
    GGCTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAACC CTGGGCCTCA GGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCCCGGGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GAGGAAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGAAT CCAGGGTAAT CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGTA TGAAGGGACC CAGTGGAGTA
    CCAGGTTCAG CTGGCCCTGA GGGGGATCCA GGACTTGTTG GCCCACCAGG TCCCCCTGGA
    CTACCTGGTC CTTCAGGACA GAGTATTATA ATCAAAGGAG ATGTTGGTCC ACCAGGGATC
    CCAGGCCAGC CTGGATTAAA AGGTCTACCA GGACTACCAG GACCTCAGGG CTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AACGGACTCC CTGGCTTTGA TGGCGCTGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGC CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGACCAG ATGGAATGCA AGGTCCTCCA GGTCCTCCAG GAACTTCCTC TATTGCCCAT
    GGATTCCTCA TCACACGTCA CAGCCAGACA ACAGATGCAC CACAATGCCC ACATGGAACT
    GTCCAGATTT ATGAAGGCTT TTCTCTCTTA TATGTCCAAG GAAATAAAAG AGCTCACGGT
    CAAGACTTGG GGACGGCTGG CAGTTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ACAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTGTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGGAG CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG CGAAGCTCCA GCTGTGGTGA TTGCAGTTCA CAGTCAGACC
    ATCCAGATTC CCCATTGTCC TCACGGATGG GATTCTCTCT GGATTGGCTA TTCCTTCATG
    ATGCACACGA GCGCAGGAGC AGAGGGCTCA GGTCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCTTTCATT GAATGTCATG GGCGGGGTAC CTGCAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTAGCA ACTGTAGATG TGTCAGATAT GTTCAGCAAA
    CCTCAGTCAG AAACACTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.