HGF cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HGF
Entrez Gene ID	403441
Full Name	hepatocyte growth factor
General protein information	Preferred Names hepatocyte growth factor Names hepatocyte growth factor SF hepatopoeitin-A hepatopoietin-A scatter factor
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005630882.1	XP_005630939.1	hepatocyte growth factor isoform X1
NM_001002964.1	NP_001002964.1	hepatocyte growth factor precursor
XM_014120749.1	XP_013976224.1	hepatocyte growth factor isoform X2
XM_005630886.2	XP_005630943.1	hepatocyte growth factor isoform X3
XM_005630882.2	XP_005630939.1	hepatocyte growth factor isoform X1
XM_022405057.1	XP_022260765.1	hepatocyte growth factor isoform X2
XM_005630886.3	XP_005630943.1	hepatocyte growth factor isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HGF	NP_001002964.1
Rattus norvegicus (Norway rat)	Hgf	NP_058713.1
	HGF	XP_001108226.2
Bos taurus (cattle)	HGF	NP_001026921.1
Homo sapiens (human)	HGF	NP_000592.3
Pan troglodytes (chimpanzee)	HGF	XP_519174.2
Mus musculus (house mouse)	Hgf	NP_034557.3
Xenopus tropicalis (tropical clawed frog)	hgf	XP_002933142.2
Gallus gallus (chicken)	HGF	NP_001025541.2
Danio rerio (zebrafish)	hgfa	NP_001013292.1

Gene Ontology
Bibliography

Related articles in PubMed

Numb is a negative regulator of HGF dependent cell scattering and Rac1 activation.
Lau KM, McGlade CJ
Experimental cell research317(4)539-51(2011 Feb)

Role of Src signal transduction pathways in scatter factor-mediated cellular protection.
Fan S, Meng Q, Laterra JJ, Rosen EM
The Journal of biological chemistry284(12)7561-77(2009 Mar)

Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells.
Kang JW, Kang KS, Koo HC, Park JR, Choi EW, Park YH
Stem cells and development17(4)681-93(2008 Aug)

Involvement of the Ras-Ras-activated Rab5 guanine nucleotide exchange factor RIN2-Rab5 pathway in the hepatocyte growth factor-induced endocytosis of E-cadherin.
Kimura T, Sakisaka T, Baba T, Yamada T, Takai Y
The Journal of biological chemistry281(15)10598-609(2006 Apr)

Canine hepatocyte growth factor: molecular cloning and characterization of the recombinant protein.
Miyake M, Saze K, Yaguchi T, Wang J, Suzuta Y, Haga Y, Takahashi SY, Yamamoto Y, Iwabuchi S
Veterinary immunology and immunopathology95(3-4)135-43(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Numb acts to suppress Rac1-GTP accumulation, and its loss leads to increased sensitivity toward extracellular signals that disrupt cell-cell adhesion to induce epithelial-mesenchymal transition (EMT) and cell dispersal
Title: Numb is a negative regulator of HGF dependent cell scattering and Rac1 activation.

as a mediator of SF- and Src-stimulated NF-kappaB activity. Finally, the Src/Rac1/MKK3/6/p38 and Src/TAK1/NF-kappaB-inducing kinase pathways exhibited cross-talk at the level of MKK3.
Title: Role of Src signal transduction pathways in scatter factor-mediated cellular protection.

Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10.
Title: Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells.

RIN2 connects Ras to Rab5 in the HGF-induced endocytosis of E-cadherin [RIN2]
Title: Involvement of the Ras-Ras-activated Rab5 guanine nucleotide exchange factor RIN2-Rab5 pathway in the hepatocyte growth factor-induced endocytosis of E-cadherin.

The following HGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe19608	XM_005630886.3 Latest version!	Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OCe00117	NM_001002964.1 Latest version!	Canis lupus familiaris hepatocyte growth factor (HGF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe19604	XM_005630882.2 Latest version!	Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe294575	XM_022405057.1 Latest version!	Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe40972	XM_014120749.1 Latest version!	Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe19604	XM_005630882.1	Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe19608	XM_005630886.2	Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HGF ( XM_005630886.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630943.1 Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe19608
Clone ID Related Accession (Same CDS sequence)	XM_005630886.2 , XM_005630886.3
Accession Version	XM_005630886.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hepatocyte growth factor isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGACATA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630943.1
CDS	148..1023
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCDIT

Target ORF information:

RefSeq Version	XM_005630886.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630886.2

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGACATA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGF ( XM_005630886.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630943.1 Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe19608
Clone ID Related Accession (Same CDS sequence)	XM_005630886.2 , XM_005630886.3
Accession Version	XM_005630886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hepatocyte growth factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGACATA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630943.1
CDS	154..1029
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCDIT

Target ORF information:

RefSeq Version	XM_005630886.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630886.3

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGACATA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGF ( NM_001002964.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002964.1 Canis lupus familiaris hepatocyte growth factor (HGF), mRNA.

Price & Availability↑

CloneID	OCe00117
Clone ID Related Accession (Same CDS sequence)	NM_001002964.1
Accession Version	NM_001002964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Canis lupus familiaris(dog)
Product	hepatocyte growth factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY543632.1. ##Evidence-Data-START## Transcript exon combination :: AY543632.1, AB090353.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001 [ECO:0000348] ##Evidence-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGGACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGCTCAC AGTACTATGA ATGATACAGA TGTCCCTATG 
GAAACAACTG AATGCATTCA AGGTCAAGGA GAAGGTTACC GGGGCACCAT CAATACCATT 
TGGAATGGAG TTCCGTGTCA GCGTTGGGAT TCCCAGTATC CTCACCAGCA TGACATAACT 
CCTGAAAATT TCAAGTGCAA GGACCTAAGA GAAAATTATT GCCGAAATCC AGATGGGGCT 
GAGTCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT 
CCAAAATGTG ATGTGTCAAG TGGACAAGAT TGTTATCGGG GGAATGGCAA AAATTATATG 
GGCAATTTAT CCAAAACACG ATCTGGACTA ACATGTTCAA TGTGGGAGAA GAACATGGAA 
GACTTACATA GGCATATCTT CTGGGAACCA GATGCTAGTA AGCTGAATAA GAATTACTGC 
CGGAATCCTG ATGACGATGC CCATGGTCCC TGGTGTTACA CGGGAAATCC TCTCATTCCA 
TGGGATTATT GTCCTATTTT TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA 
GACCATCCTG TAATATCTTG TGCCAAAACA AAACAATTAC GAGTTGTAAA TGGAATTCCA 
ACACGGACTA ATGTAGGATG GATGGTTAGT TTGAAATACA GAAATAAACA TATCTGTGGA 
GGATCATTGA TAAAGGAAAG TTGGATTCTT ACTGCAAGAC AATGTTTCCC CTCTCGAAAC 
AGAGACTTGA AAGATTATGA AGCTTGGCTT GGGATTCATG ACGTCCACGG AAAAGGAGAT 
GAGAAACGCA AACAGGTTCT GAATGTTTCC CAGCTGGTAT ATGGGCCTGA AGGATCAGAT 
CTGGTATTAC TGAAGCTTGC TAGGCCCGCT ATCCTGGATG ATTTTGTTAG TACAATCGAT 
TTACCTAATT ATGGATGCAC CATTCCTGAA AAAACCACTT GCAGTGTTTA TGGCTGGGGT 
TATACTGGAT CGATCAACTT TGATGGTCTA TTACGAGTAG CACATCTCTA TATTATGGGG 
AATGAGAAAT GCAGCCAATA CCATCAAGGG AAGGTGACAC TGAATGAGTC TGAAATATGT 
GCTGGAGCTG AAAATATTGT ATCAGGACCA TGTGAGGGAG ATTATGGTGG CCCACTTGTT 
TGCGAACAAC ATAAAATGAG GATGGTTCTT GGCGTCATTG TTCCTGGTCG TGGATGTGCC 
ATTCCAAATC GTCCTGGCAT TTTTGTCCGA GTAGCATATT ATGCAAAATG GATACACAAA 
ATTATATTAA CGTATAAGAT ACAACAGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002964.1
CDS	1..2193
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDVPMETTECIQGQGEGYRGTINTIWNGVPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWEKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPIFRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNRDLKDYEAWLGIHDVHGKGDEKRKQVLNVSQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGSINFDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKIQQS

Target ORF information:

RefSeq Version	NM_001002964.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hepatocyte growth factor (HGF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002964.1

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGGACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGCTCAC AGTACTATGA ATGATACAGA TGTCCCTATG 
GAAACAACTG AATGCATTCA AGGTCAAGGA GAAGGTTACC GGGGCACCAT CAATACCATT 
TGGAATGGAG TTCCGTGTCA GCGTTGGGAT TCCCAGTATC CTCACCAGCA TGACATAACT 
CCTGAAAATT TCAAGTGCAA GGACCTAAGA GAAAATTATT GCCGAAATCC AGATGGGGCT 
GAGTCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT 
CCAAAATGTG ATGTGTCAAG TGGACAAGAT TGTTATCGGG GGAATGGCAA AAATTATATG 
GGCAATTTAT CCAAAACACG ATCTGGACTA ACATGTTCAA TGTGGGAGAA GAACATGGAA 
GACTTACATA GGCATATCTT CTGGGAACCA GATGCTAGTA AGCTGAATAA GAATTACTGC 
CGGAATCCTG ATGACGATGC CCATGGTCCC TGGTGTTACA CGGGAAATCC TCTCATTCCA 
TGGGATTATT GTCCTATTTT TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA 
GACCATCCTG TAATATCTTG TGCCAAAACA AAACAATTAC GAGTTGTAAA TGGAATTCCA 
ACACGGACTA ATGTAGGATG GATGGTTAGT TTGAAATACA GAAATAAACA TATCTGTGGA 
GGATCATTGA TAAAGGAAAG TTGGATTCTT ACTGCAAGAC AATGTTTCCC CTCTCGAAAC 
AGAGACTTGA AAGATTATGA AGCTTGGCTT GGGATTCATG ACGTCCACGG AAAAGGAGAT 
GAGAAACGCA AACAGGTTCT GAATGTTTCC CAGCTGGTAT ATGGGCCTGA AGGATCAGAT 
CTGGTATTAC TGAAGCTTGC TAGGCCCGCT ATCCTGGATG ATTTTGTTAG TACAATCGAT 
TTACCTAATT ATGGATGCAC CATTCCTGAA AAAACCACTT GCAGTGTTTA TGGCTGGGGT 
TATACTGGAT CGATCAACTT TGATGGTCTA TTACGAGTAG CACATCTCTA TATTATGGGG 
AATGAGAAAT GCAGCCAATA CCATCAAGGG AAGGTGACAC TGAATGAGTC TGAAATATGT 
GCTGGAGCTG AAAATATTGT ATCAGGACCA TGTGAGGGAG ATTATGGTGG CCCACTTGTT 
TGCGAACAAC ATAAAATGAG GATGGTTCTT GGCGTCATTG TTCCTGGTCG TGGATGTGCC 
ATTCCAAATC GTCCTGGCAT TTTTGTCCGA GTAGCATATT ATGCAAAATG GATACACAAA 
ATTATATTAA CGTATAAGAT ACAACAGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGF ( XM_005630882.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630939.1 Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe19604
Clone ID Related Accession (Same CDS sequence)	XM_005630882.1 , XM_005630882.2
Accession Version	XM_005630882.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hepatocyte growth factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG 
GGACCTTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG 
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAAAGTT ATCGAGGTCC CATGGATCAC 
ACAGAATCGG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCGCACCG GCACAAATTC 
TTGCCGGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA CCCTGATGGC 
AAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTGACACCC CCTGGGAGTA CTGTGCAATT 
AAAATGTGTG CTCACAGTAC TATGAATGAT ACAGATGTCC CTATGGAAAC AACTGAATGC 
ATTCAAGGTC AAGGAGAAGG TTACCGGGGC ACCATCAATA CCATTTGGAA TGGAGTTCCG 
TGTCAGCGTT GGGATTCCCA GTATCCTCAC CAGCATGACA TAACTCCTGA AAATTTCAAG 
TGCAAGGACC TAAGAGAAAA TTATTGCCGA AATCCAGATG GGGCTGAGTC ACCCTGGTGT 
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA ATGTGATGTG 
TCAAGTGGAC AAGATTGTTA TCGGGGGAAT GGCAAAAATT ATATGGGCAA TTTATCCAAA 
ACACGATCTG GACTAACATG TTCAATGTGG GAGAAGAACA TGGAAGACTT ACATAGGCAT 
ATCTTCTGGG AACCAGATGC TAGTAAGCTG AATAAGAATT ACTGCCGGAA TCCTGATGAC 
GATGCCCATG GTCCCTGGTG TTACACGGGA AATCCTCTCA TTCCATGGGA TTATTGTCCT 
ATTTTTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCTGTAATA 
TCTTGTGCCA AAACAAAACA ATTACGAGTT GTAAATGGAA TTCCAACACG GACTAATGTA 
GGATGGATGG TTAGTTTGAA ATACAGAAAT AAACATATCT GTGGAGGATC ATTGATAAAG 
GAAAGTTGGA TTCTTACTGC AAGACAATGT TTCCCCTCTC GAAACAGAGA CTTGAAAGAT 
TATGAAGCTT GGCTTGGGAT TCATGACGTC CACGGAAAAG GAGATGAGAA ACGCAAACAG 
GTTCTGAATG TTTCCCAGCT GGTATATGGG CCTGAAGGAT CAGATCTGGT ATTACTGAAG 
CTTGCTAGGC CCGCTATCCT GGATGATTTT GTTAGTACAA TCGATTTACC TAATTATGGA 
TGCACCATTC CTGAAAAAAC CACTTGCAGT GTTTATGGCT GGGGTTATAC TGGATCGATC 
AACTTTGATG GTCTATTACG AGTAGCACAT CTCTATATTA TGGGGAATGA GAAATGCAGC 
CAATACCATC AAGGGAAGGT GACACTGAAT GAGTCTGAAA TATGTGCTGG AGCTGAAAAT 
ATTGTATCAG GACCATGTGA GGGAGATTAT GGTGGCCCAC TTGTTTGCGA ACAACATAAA 
ATGAGGATGG TTCTTGGCGT CATTGTTCCT GGTCGTGGAT GTGCCATTCC AAATCGTCCT 
GGCATTTTTG TCCGAGTAGC ATATTATGCA AAATGGATAC ACAAAATTAT ATTAACGTAT 
AAGATACAAC AGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630939.1
CDS	148..2325
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDVPMETTECIQGQGEGYRGTINTIWNGVPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWEKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPIFRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNRDLKDYEAWLGIHDVHGKGDEKRKQVLNVSQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGSINFDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKIQQS

Target ORF information:

RefSeq Version	XM_005630882.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630882.1

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG 
GGACCTTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG 
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAAAGTT ATCGAGGTCC CATGGATCAC 
ACAGAATCGG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCGCACCG GCACAAATTC 
TTGCCGGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA CCCTGATGGC 
AAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTGACACCC CCTGGGAGTA CTGTGCAATT 
AAAATGTGTG CTCACAGTAC TATGAATGAT ACAGATGTCC CTATGGAAAC AACTGAATGC 
ATTCAAGGTC AAGGAGAAGG TTACCGGGGC ACCATCAATA CCATTTGGAA TGGAGTTCCG 
TGTCAGCGTT GGGATTCCCA GTATCCTCAC CAGCATGACA TAACTCCTGA AAATTTCAAG 
TGCAAGGACC TAAGAGAAAA TTATTGCCGA AATCCAGATG GGGCTGAGTC ACCCTGGTGT 
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA ATGTGATGTG 
TCAAGTGGAC AAGATTGTTA TCGGGGGAAT GGCAAAAATT ATATGGGCAA TTTATCCAAA 
ACACGATCTG GACTAACATG TTCAATGTGG GAGAAGAACA TGGAAGACTT ACATAGGCAT 
ATCTTCTGGG AACCAGATGC TAGTAAGCTG AATAAGAATT ACTGCCGGAA TCCTGATGAC 
GATGCCCATG GTCCCTGGTG TTACACGGGA AATCCTCTCA TTCCATGGGA TTATTGTCCT 
ATTTTTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCTGTAATA 
TCTTGTGCCA AAACAAAACA ATTACGAGTT GTAAATGGAA TTCCAACACG GACTAATGTA 
GGATGGATGG TTAGTTTGAA ATACAGAAAT AAACATATCT GTGGAGGATC ATTGATAAAG 
GAAAGTTGGA TTCTTACTGC AAGACAATGT TTCCCCTCTC GAAACAGAGA CTTGAAAGAT 
TATGAAGCTT GGCTTGGGAT TCATGACGTC CACGGAAAAG GAGATGAGAA ACGCAAACAG 
GTTCTGAATG TTTCCCAGCT GGTATATGGG CCTGAAGGAT CAGATCTGGT ATTACTGAAG 
CTTGCTAGGC CCGCTATCCT GGATGATTTT GTTAGTACAA TCGATTTACC TAATTATGGA 
TGCACCATTC CTGAAAAAAC CACTTGCAGT GTTTATGGCT GGGGTTATAC TGGATCGATC 
AACTTTGATG GTCTATTACG AGTAGCACAT CTCTATATTA TGGGGAATGA GAAATGCAGC 
CAATACCATC AAGGGAAGGT GACACTGAAT GAGTCTGAAA TATGTGCTGG AGCTGAAAAT 
ATTGTATCAG GACCATGTGA GGGAGATTAT GGTGGCCCAC TTGTTTGCGA ACAACATAAA 
ATGAGGATGG TTCTTGGCGT CATTGTTCCT GGTCGTGGAT GTGCCATTCC AAATCGTCCT 
GGCATTTTTG TCCGAGTAGC ATATTATGCA AAATGGATAC ACAAAATTAT ATTAACGTAT 
AAGATACAAC AGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGF ( XM_005630882.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630939.1 Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe19604
Clone ID Related Accession (Same CDS sequence)	XM_005630882.1 , XM_005630882.2
Accession Version	XM_005630882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hepatocyte growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG 
GGACCTTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG 
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAAAGTT ATCGAGGTCC CATGGATCAC 
ACAGAATCGG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCGCACCG GCACAAATTC 
TTGCCGGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA CCCTGATGGC 
AAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTGACACCC CCTGGGAGTA CTGTGCAATT 
AAAATGTGTG CTCACAGTAC TATGAATGAT ACAGATGTCC CTATGGAAAC AACTGAATGC 
ATTCAAGGTC AAGGAGAAGG TTACCGGGGC ACCATCAATA CCATTTGGAA TGGAGTTCCG 
TGTCAGCGTT GGGATTCCCA GTATCCTCAC CAGCATGACA TAACTCCTGA AAATTTCAAG 
TGCAAGGACC TAAGAGAAAA TTATTGCCGA AATCCAGATG GGGCTGAGTC ACCCTGGTGT 
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA ATGTGATGTG 
TCAAGTGGAC AAGATTGTTA TCGGGGGAAT GGCAAAAATT ATATGGGCAA TTTATCCAAA 
ACACGATCTG GACTAACATG TTCAATGTGG GAGAAGAACA TGGAAGACTT ACATAGGCAT 
ATCTTCTGGG AACCAGATGC TAGTAAGCTG AATAAGAATT ACTGCCGGAA TCCTGATGAC 
GATGCCCATG GTCCCTGGTG TTACACGGGA AATCCTCTCA TTCCATGGGA TTATTGTCCT 
ATTTTTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCTGTAATA 
TCTTGTGCCA AAACAAAACA ATTACGAGTT GTAAATGGAA TTCCAACACG GACTAATGTA 
GGATGGATGG TTAGTTTGAA ATACAGAAAT AAACATATCT GTGGAGGATC ATTGATAAAG 
GAAAGTTGGA TTCTTACTGC AAGACAATGT TTCCCCTCTC GAAACAGAGA CTTGAAAGAT 
TATGAAGCTT GGCTTGGGAT TCATGACGTC CACGGAAAAG GAGATGAGAA ACGCAAACAG 
GTTCTGAATG TTTCCCAGCT GGTATATGGG CCTGAAGGAT CAGATCTGGT ATTACTGAAG 
CTTGCTAGGC CCGCTATCCT GGATGATTTT GTTAGTACAA TCGATTTACC TAATTATGGA 
TGCACCATTC CTGAAAAAAC CACTTGCAGT GTTTATGGCT GGGGTTATAC TGGATCGATC 
AACTTTGATG GTCTATTACG AGTAGCACAT CTCTATATTA TGGGGAATGA GAAATGCAGC 
CAATACCATC AAGGGAAGGT GACACTGAAT GAGTCTGAAA TATGTGCTGG AGCTGAAAAT 
ATTGTATCAG GACCATGTGA GGGAGATTAT GGTGGCCCAC TTGTTTGCGA ACAACATAAA 
ATGAGGATGG TTCTTGGCGT CATTGTTCCT GGTCGTGGAT GTGCCATTCC AAATCGTCCT 
GGCATTTTTG TCCGAGTAGC ATATTATGCA AAATGGATAC ACAAAATTAT ATTAACGTAT 
AAGATACAAC AGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630939.1
CDS	154..2331
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDVPMETTECIQGQGEGYRGTINTIWNGVPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWEKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPIFRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNRDLKDYEAWLGIHDVHGKGDEKRKQVLNVSQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGSINFDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKIQQS

Target ORF information:

RefSeq Version	XM_005630882.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630882.2

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG 
GGACCTTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG 
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAAAGTT ATCGAGGTCC CATGGATCAC 
ACAGAATCGG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCGCACCG GCACAAATTC 
TTGCCGGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA CCCTGATGGC 
AAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTGACACCC CCTGGGAGTA CTGTGCAATT 
AAAATGTGTG CTCACAGTAC TATGAATGAT ACAGATGTCC CTATGGAAAC AACTGAATGC 
ATTCAAGGTC AAGGAGAAGG TTACCGGGGC ACCATCAATA CCATTTGGAA TGGAGTTCCG 
TGTCAGCGTT GGGATTCCCA GTATCCTCAC CAGCATGACA TAACTCCTGA AAATTTCAAG 
TGCAAGGACC TAAGAGAAAA TTATTGCCGA AATCCAGATG GGGCTGAGTC ACCCTGGTGT 
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA ATGTGATGTG 
TCAAGTGGAC AAGATTGTTA TCGGGGGAAT GGCAAAAATT ATATGGGCAA TTTATCCAAA 
ACACGATCTG GACTAACATG TTCAATGTGG GAGAAGAACA TGGAAGACTT ACATAGGCAT 
ATCTTCTGGG AACCAGATGC TAGTAAGCTG AATAAGAATT ACTGCCGGAA TCCTGATGAC 
GATGCCCATG GTCCCTGGTG TTACACGGGA AATCCTCTCA TTCCATGGGA TTATTGTCCT 
ATTTTTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCTGTAATA 
TCTTGTGCCA AAACAAAACA ATTACGAGTT GTAAATGGAA TTCCAACACG GACTAATGTA 
GGATGGATGG TTAGTTTGAA ATACAGAAAT AAACATATCT GTGGAGGATC ATTGATAAAG 
GAAAGTTGGA TTCTTACTGC AAGACAATGT TTCCCCTCTC GAAACAGAGA CTTGAAAGAT 
TATGAAGCTT GGCTTGGGAT TCATGACGTC CACGGAAAAG GAGATGAGAA ACGCAAACAG 
GTTCTGAATG TTTCCCAGCT GGTATATGGG CCTGAAGGAT CAGATCTGGT ATTACTGAAG 
CTTGCTAGGC CCGCTATCCT GGATGATTTT GTTAGTACAA TCGATTTACC TAATTATGGA 
TGCACCATTC CTGAAAAAAC CACTTGCAGT GTTTATGGCT GGGGTTATAC TGGATCGATC 
AACTTTGATG GTCTATTACG AGTAGCACAT CTCTATATTA TGGGGAATGA GAAATGCAGC 
CAATACCATC AAGGGAAGGT GACACTGAAT GAGTCTGAAA TATGTGCTGG AGCTGAAAAT 
ATTGTATCAG GACCATGTGA GGGAGATTAT GGTGGCCCAC TTGTTTGCGA ACAACATAAA 
ATGAGGATGG TTCTTGGCGT CATTGTTCCT GGTCGTGGAT GTGCCATTCC AAATCGTCCT 
GGCATTTTTG TCCGAGTAGC ATATTATGCA AAATGGATAC ACAAAATTAT ATTAACGTAT 
AAGATACAAC AGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGF ( XM_022405057.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260765.1 Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe294575
Clone ID Related Accession (Same CDS sequence)	XM_022405057.1
Accession Version	XM_022405057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hepatocyte growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGCTCAC AGTACTATGA ATGATACAGA TGTCCCTATG 
GAAACAACTG AATGCATTCA AGGTCAAGGA GAAGGTTACC GGGGCACCAT CAATACCATT 
TGGAATGGAG TTCCGTGTCA GCGTTGGGAT TCCCAGTATC CTCACCAGCA TGACATAACT 
CCTGAAAATT TCAAGTGCAA GGACCTAAGA GAAAATTATT GCCGAAATCC AGATGGGGCT 
GAGTCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT 
CCAAAATGTG ATGTGTCAAG TGGACAAGAT TGTTATCGGG GGAATGGCAA AAATTATATG 
GGCAATTTAT CCAAAACACG ATCTGGACTA ACATGTTCAA TGTGGGAGAA GAACATGGAA 
GACTTACATA GGCATATCTT CTGGGAACCA GATGCTAGTA AGCTGAATAA GAATTACTGC 
CGGAATCCTG ATGACGATGC CCATGGTCCC TGGTGTTACA CGGGAAATCC TCTCATTCCA 
TGGGATTATT GTCCTATTTT TCGTTATCCT GTAATATCTT GTGCCAAAAC AAAACAATTA 
CGAGTTGTAA ATGGAATTCC AACACGGACT AATGTAGGAT GGATGGTTAG TTTGAAATAC 
AGAAATAAAC ATATCTGTGG AGGATCATTG ATAAAGGAAA GTTGGATTCT TACTGCAAGA 
CAATGTTTCC CCTCTCGAAA CAGAGACTTG AAAGATTATG AAGCTTGGCT TGGGATTCAT 
GACGTCCACG GAAAAGGAGA TGAGAAACGC AAACAGGTTC TGAATGTTTC CCAGCTGGTA 
TATGGGCCTG AAGGATCAGA TCTGGTATTA CTGAAGCTTG CTAGGCCCGC TATCCTGGAT 
GATTTTGTTA GTACAATCGA TTTACCTAAT TATGGATGCA CCATTCCTGA AAAAACCACT 
TGCAGTGTTT ATGGCTGGGG TTATACTGGA TCGATCAACT TTGATGGTCT ATTACGAGTA 
GCACATCTCT ATATTATGGG GAATGAGAAA TGCAGCCAAT ACCATCAAGG GAAGGTGACA 
CTGAATGAGT CTGAAATATG TGCTGGAGCT GAAAATATTG TATCAGGACC ATGTGAGGGA 
GATTATGGTG GCCCACTTGT TTGCGAACAA CATAAAATGA GGATGGTTCT TGGCGTCATT 
GTTCCTGGTC GTGGATGTGC CATTCCAAAT CGTCCTGGCA TTTTTGTCCG AGTAGCATAT 
TATGCAAAAT GGATACACAA AATTATATTA ACGTATAAGA TACAACAGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260765.1
CDS	154..2307
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDVPMETTECIQGQGEGYRGTINTIWNGVPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWEKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPIFRYPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNRDLKDYEAWLGIHDVHGKGDEKRKQVLNVSQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGSINFDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKIQQS

Target ORF information:

RefSeq Version	XM_022405057.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hepatocyte growth factor (HGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405057.1

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGCTCAC AGTACTATGA ATGATACAGA TGTCCCTATG 
GAAACAACTG AATGCATTCA AGGTCAAGGA GAAGGTTACC GGGGCACCAT CAATACCATT 
TGGAATGGAG TTCCGTGTCA GCGTTGGGAT TCCCAGTATC CTCACCAGCA TGACATAACT 
CCTGAAAATT TCAAGTGCAA GGACCTAAGA GAAAATTATT GCCGAAATCC AGATGGGGCT 
GAGTCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT 
CCAAAATGTG ATGTGTCAAG TGGACAAGAT TGTTATCGGG GGAATGGCAA AAATTATATG 
GGCAATTTAT CCAAAACACG ATCTGGACTA ACATGTTCAA TGTGGGAGAA GAACATGGAA 
GACTTACATA GGCATATCTT CTGGGAACCA GATGCTAGTA AGCTGAATAA GAATTACTGC 
CGGAATCCTG ATGACGATGC CCATGGTCCC TGGTGTTACA CGGGAAATCC TCTCATTCCA 
TGGGATTATT GTCCTATTTT TCGTTATCCT GTAATATCTT GTGCCAAAAC AAAACAATTA 
CGAGTTGTAA ATGGAATTCC AACACGGACT AATGTAGGAT GGATGGTTAG TTTGAAATAC 
AGAAATAAAC ATATCTGTGG AGGATCATTG ATAAAGGAAA GTTGGATTCT TACTGCAAGA 
CAATGTTTCC CCTCTCGAAA CAGAGACTTG AAAGATTATG AAGCTTGGCT TGGGATTCAT 
GACGTCCACG GAAAAGGAGA TGAGAAACGC AAACAGGTTC TGAATGTTTC CCAGCTGGTA 
TATGGGCCTG AAGGATCAGA TCTGGTATTA CTGAAGCTTG CTAGGCCCGC TATCCTGGAT 
GATTTTGTTA GTACAATCGA TTTACCTAAT TATGGATGCA CCATTCCTGA AAAAACCACT 
TGCAGTGTTT ATGGCTGGGG TTATACTGGA TCGATCAACT TTGATGGTCT ATTACGAGTA 
GCACATCTCT ATATTATGGG GAATGAGAAA TGCAGCCAAT ACCATCAAGG GAAGGTGACA 
CTGAATGAGT CTGAAATATG TGCTGGAGCT GAAAATATTG TATCAGGACC ATGTGAGGGA 
GATTATGGTG GCCCACTTGT TTGCGAACAA CATAAAATGA GGATGGTTCT TGGCGTCATT 
GTTCCTGGTC GTGGATGTGC CATTCCAAAT CGTCCTGGCA TTTTTGTCCG AGTAGCATAT 
TATGCAAAAT GGATACACAA AATTATATTA ACGTATAAGA TACAACAGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HGF ( XM_014120749.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976224.1 Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X2, mRNA.

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CloneID	OCe40972
Clone ID Related Accession (Same CDS sequence)	XM_014120749.1
Accession Version	XM_014120749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hepatocyte growth factor isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGCTCAC AGTACTATGA ATGATACAGA TGTCCCTATG 
GAAACAACTG AATGCATTCA AGGTCAAGGA GAAGGTTACC GGGGCACCAT CAATACCATT 
TGGAATGGAG TTCCGTGTCA GCGTTGGGAT TCCCAGTATC CTCACCAGCA TGACATAACT 
CCTGAAAATT TCAAGTGCAA GGACCTAAGA GAAAATTATT GCCGAAATCC AGATGGGGCT 
GAGTCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT 
CCAAAATGTG ATGTGTCAAG TGGACAAGAT TGTTATCGGG GGAATGGCAA AAATTATATG 
GGCAATTTAT CCAAAACACG ATCTGGACTA ACATGTTCAA TGTGGGAGAA GAACATGGAA 
GACTTACATA GGCATATCTT CTGGGAACCA GATGCTAGTA AGCTGAATAA GAATTACTGC 
CGGAATCCTG ATGACGATGC CCATGGTCCC TGGTGTTACA CGGGAAATCC TCTCATTCCA 
TGGGATTATT GTCCTATTTT TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA 
GACCATCCTG TAATATCTTG TGCCAAAACA AAACAATTAC GAGTTGTAAA TGGAATTCCA 
ACACGGACTA ATGTAGGATG GATGGTTAGT TTGAAATACA GAAATAAACA TATCTGTGGA 
GGATCATTGA TAAAGGAAAG TTGGATTCTT ACTGCAAGAC AATGTTTCCC CTCTCGGCCC 
GCTATCCTGG ATGATTTTGT TAGTACAATC GATTTACCTA ATTATGGATG CACCATTCCT 
GAAAAAACCA CTTGCAGTGT TTATGGCTGG GGTTATACTG GATCGATCAA CTTTGATGGT 
CTATTACGAG TAGCACATCT CTATATTATG GGGAATGAGA AATGCAGCCA ATACCATCAA 
GGGAAGGTGA CACTGAATGA GTCTGAAATA TGTGCTGGAG CTGAAAATAT TGTATCAGGA 
CCATGTGAGG GAGATTATGG TGGCCCACTT GTTTGCGAAC AACATAAAAT GAGGATGGTT 
CTTGGCGTCA TTGTTCCTGG TCGTGGATGT GCCATTCCAA ATCGTCCTGG CATTTTTGTC 
CGAGTAGCAT ATTATGCAAA ATGGATACAC AAAATTATAT TAACGTATAA GATACAACAG 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976224.1
CDS	148..2193
Translation	MWVTKLLPLLVLQQLLLHLLLLPVAVPRAEGQKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDVPMETTECIQGQGEGYRGTINTIWNGVPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWEKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPIFRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGSINFDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKIQQS

Target ORF information:

RefSeq Version	XM_014120749.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120749.1

ORF Insert Sequence:

ATGTGGGTGA CCAAGCTCCT GCCCCTGCTG GTGCTGCAGC AGCTCCTCCT GCACCTCCTC 
CTGCTGCCCG TCGCCGTCCC CCGTGCAGAA GGACAGAAGA AAAGAAGAAA CACACTTCAT 
GAATTCAAAA AGTCAGCAAA GACTACTCTA ATTAAAGAAG ACCCATTACT GAAGATAAAA 
ACAAAAAAAA TGAACACTGC AGACCAATGT GCCAATAGAT GTATTAGGAA TAAAGGACTT 
CCATTCACTT GCAAGGCCTT TGTTTTTGAT AAAGCAAGGA AACGATGCCT CTGGTTCCCT 
TTCAATAGCA TGACAAGTGG AGTGAAAAAA GAGTTTGGTC ATGAATTTGA TCTCTATGAA 
AACAAAGACT ACATTAGGAA CTGCATCATT GGTAAAGGAG GTAGCTACAA GGGAACAGTG 
TCTATCACTA AGAGTGGCAT CAAATGCCAG CCCTGGAATT CCATGATACC ACATGAACAC 
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT 
CGAGGGGAAG AAGGGGGACC TTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC 
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA AAGTTATCGA 
GGTCCCATGG ATCACACAGA ATCGGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCG 
CACCGGCACA AATTCTTGCC GGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC 
CGCAACCCTG ATGGCAAGCC GAGGCCATGG TGCTATACTC TTGACCCTGA CACCCCCTGG 
GAGTACTGTG CAATTAAAAT GTGTGCTCAC AGTACTATGA ATGATACAGA TGTCCCTATG 
GAAACAACTG AATGCATTCA AGGTCAAGGA GAAGGTTACC GGGGCACCAT CAATACCATT 
TGGAATGGAG TTCCGTGTCA GCGTTGGGAT TCCCAGTATC CTCACCAGCA TGACATAACT 
CCTGAAAATT TCAAGTGCAA GGACCTAAGA GAAAATTATT GCCGAAATCC AGATGGGGCT 
GAGTCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT 
CCAAAATGTG ATGTGTCAAG TGGACAAGAT TGTTATCGGG GGAATGGCAA AAATTATATG 
GGCAATTTAT CCAAAACACG ATCTGGACTA ACATGTTCAA TGTGGGAGAA GAACATGGAA 
GACTTACATA GGCATATCTT CTGGGAACCA GATGCTAGTA AGCTGAATAA GAATTACTGC 
CGGAATCCTG ATGACGATGC CCATGGTCCC TGGTGTTACA CGGGAAATCC TCTCATTCCA 
TGGGATTATT GTCCTATTTT TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA 
GACCATCCTG TAATATCTTG TGCCAAAACA AAACAATTAC GAGTTGTAAA TGGAATTCCA 
ACACGGACTA ATGTAGGATG GATGGTTAGT TTGAAATACA GAAATAAACA TATCTGTGGA 
GGATCATTGA TAAAGGAAAG TTGGATTCTT ACTGCAAGAC AATGTTTCCC CTCTCGGCCC 
GCTATCCTGG ATGATTTTGT TAGTACAATC GATTTACCTA ATTATGGATG CACCATTCCT 
GAAAAAACCA CTTGCAGTGT TTATGGCTGG GGTTATACTG GATCGATCAA CTTTGATGGT 
CTATTACGAG TAGCACATCT CTATATTATG GGGAATGAGA AATGCAGCCA ATACCATCAA 
GGGAAGGTGA CACTGAATGA GTCTGAAATA TGTGCTGGAG CTGAAAATAT TGTATCAGGA 
CCATGTGAGG GAGATTATGG TGGCCCACTT GTTTGCGAAC AACATAAAAT GAGGATGGTT 
CTTGGCGTCA TTGTTCCTGG TCGTGGATGT GCCATTCCAA ATCGTCCTGG CATTTTTGTC 
CGAGTAGCAT ATTATGCAAA ATGGATACAC AAAATTATAT TAACGTATAA GATACAACAG 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Numb is a negative regulator of HGF dependent cell scattering and Rac1 activation.
Experimental cell research317(4)539-51(2011 Feb)
Lau KM,McGlade CJ

Role of Src signal transduction pathways in scatter factor-mediated cellular protection.
The Journal of biological chemistry284(12)7561-77(2009 Mar)
Fan S,Meng Q,Laterra JJ,Rosen EM

Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells.
Stem cells and development17(4)681-93(2008 Aug)
Kang JW,Kang KS,Koo HC,Park JR,Choi EW,Park YH

Involvement of the Ras-Ras-activated Rab5 guanine nucleotide exchange factor RIN2-Rab5 pathway in the hepatocyte growth factor-induced endocytosis of E-cadherin.
The Journal of biological chemistry281(15)10598-609(2006 Apr)
Kimura T,Sakisaka T,Baba T,Yamada T,Takai Y

Canine hepatocyte growth factor: molecular cloning and characterization of the recombinant protein.
Veterinary immunology and immunopathology95(3-4)135-43(2003 Oct)
Miyake M,Saze K,Yaguchi T,Wang J,Suzuta Y,Haga Y,Takahashi SY,Yamamoto Y,Iwabuchi S

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HGF cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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