GJA1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJA1
Entrez Gene ID	403418
Full Name	gap junction protein alpha 1
Synonyms	cx43
General protein information	Preferred Names gap junction protein alpha 1 Names gap junction alpha-1 protein gap junction protein, alpha 1, 43kDa (connexin 43)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002951.3	NP_001002951.2	gap junction alpha-1 protein

Pathways from BioSystems

Homologies

	GJA1	XP_001109617.1
Mus musculus (house mouse)	Gja1	NP_034418.1
Xenopus tropicalis (tropical clawed frog)	gja1	NP_988856.1
Pan troglodytes (chimpanzee)	GJA1	XP_001164338.1
Canis lupus familiaris (dog)	GJA1	NP_001002951.2
Bos taurus (cattle)	GJA1	NP_776493.1
Rattus norvegicus (Norway rat)	Gja1	NP_036699.1
Homo sapiens (human)	GJA1	NP_000156.1
Gallus gallus (chicken)	GJA1	NP_989917.1
Danio rerio (zebrafish)	cx43	NP_571113.1

Gene Ontology
Bibliography

Related articles in PubMed

Acetylation mediates Cx43 reduction caused by electrical stimulation.
Meraviglia V, Azzimato V, Colussi C, Florio MC, Binda A, Panariti A, Qanud K, Suffredini S, Gennaccaro L, Miragoli M, Barbuti A, Lampe PD, Gaetano C, Pramstaller PP, Capogrossi MC, Recchia FA, Pompilio G, Rivolta I, Rossini A
Journal of molecular and cellular cardiology8754-64(2015 Oct)

Dynamic alterations of connexin43, matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 during ventricular fibrillation in canine.
Wang J, Li JS, Liu HZ, Yi SL, Su GY, Zhang Y, Zhong JQ
Molecular and cellular biochemistry391(1-2)259-66(2014 Jun)

Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.
Dunn CA, Lampe PD
Journal of cell science127(Pt 2)455-64(2014 Jan)

The involvement of gap junctions in the delayed phase of the protection induced by cardiac pacing in dogs.
G?nczi M, Kov?cs M, Sepr?nyi G, V?gh A
Clinical science (London, England : 1979)123(1)39-51(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

increased acetylation of Cx43 in the left ventricles of dogs subjected to chronic tachypacing as abnormal ventricular activation... altered electrical activity can regulate cardiomyocyte communication by influencing the acetylation status of Cx43
Title: Acetylation mediates Cx43 reduction caused by electrical stimulation.

the alteration of Cx43 and/or phosphorylated Cx43 levels and the imbalance of MMP-2 and TIMP-2 may contribute to the initiation and/or persistence of ventricular fibrillation.
Title: Dynamic alterations of connexin43, matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 during ventricular fibrillation in canine.

Akt-dependent phosphorylation of Serine 373 increases gap junction size and communication by completely eliminating the interaction between Cx43 and ZO-1.
Title: Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.

Cardiac pacing results in time-dependent changes in connexin43 expression, which may alter gap junction function and influence arrhythmia generation during a subsequent ischaemia/reperfusion insult.
Title: The involvement of gap junctions in the delayed phase of the protection induced by cardiac pacing in dogs.

Cx43 ubiquitination is not necessary for the regulation of Cx43 turnover; rather, Akt activity, probably through direct phosphorylation of Cx43, controls gap junction stability.
Title: Activation of Akt, not connexin 43 protein ubiquitination, regulates gap junction stability.

The following GJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00496	NM_001002951.3 Latest version!	Canis lupus familiaris gap junction protein alpha 1 (GJA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJA1 ( NM_001002951.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002951.2 Canis lupus familiaris gap junction protein alpha 1 (GJA1), mRNA.

Price & Availability↑

CloneID	OCe00496
Clone ID Related Accession (Same CDS sequence)	NM_001002951.3
Accession Version	NM_001002951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Canis lupus familiaris(dog)
Product	gap junction alpha-1 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03000408.1. On Sep 6, 2015 this sequence version replaced NM_001002951.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGGTGACT GGAGTGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA TTCCACCGCT 
GGAGGGAAGG TGTGGCTGTC TGTCCTTTTC ATTTTCCGAA TTCTGCTACT GGGGACAGCG 
GTTGAGTCGG CCTGGGGTGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA ACAGCCTGGT 
TGTGAAAATG TCTGCTATGA CAAATCCTTC CCAATCTCTC ATGTACGCTT CTGGGTCCTG 
CAGATCATAT TTGTGTCTGT CCCCACACTC TTGTACCTGG CTCATGTGTT CTACGTGATG 
CGCAAGGAAG AGAAACTGAA CAAGAAAGAG GAGGAGCTCA AAGTTGCCCA AACCGATGGT 
GCCAACGTGG ACATGCACTT GAAGCAGATT GAAATAAAGA AGTTCAAGTA TGGAATTGAA 
GAGCACGGCA AGGTGAAAAT GCGAGGGGGC CTGCTGCGAA CCTACATCAT CAGCATCCTG 
TTCAAGTCTG TTTTCGAGGT GGCCTTCTTG CTGATCCAGT GGTACATCTA TGGATTCAGC 
TTGAGTGCCG TTTACACTTG CAAAAGAGAA CCCTGCCCTC ATCAGGTAGA CTGCTTCCTC 
TCTCGCCCCA CGGAGAAAAC CATCTTCATC ATCTTCATGC TGGTCGTGTC CTTGGTGTCT 
CTTGCCTTGA ACATCATCGA ACTCTTCTAT GTCTTCTTCA AGGGTGTTAA GGATCGCGTG 
AAAGGCAAGA GCGATCCTTA CCATGCTACC ACTGGCCCAC TGAGCCCCTC CAAAGACTGT 
GGATCTCCGA AATATGCATA TTTCAATGGC TGCTCCTCAC CCACGGCTCC CCTCTCCCCC 
ATGTCTCCTC CTGGGTACAA GCTGGTTACT GGAGACAGAA ATAATTCTTC CTGCCGCAAT 
TACAACAAAC AAGCAAGTGA GCAAAACTGG GCTAACTACA GTGCAGAACA AAATCGAATG 
GGCCAGGCAG GAAGCACCAT CTCCAACTCC CATGCACAGC CTTTTGATTT CCCTGATGAC 
AACCAGAATT CTAAAAAACT AGCCGCTGGG CATGAACTGC AGCCACTAGC CATTGTGGAC 
CAGCGGCCTT CCAGCAGAGC CAGCAGCCGT GCCAGCAGCC GACCTCGGCC TGACGACCTG 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002951.2
CDS	17..1165
Translation	MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGANVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKREPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Target ORF information:

RefSeq Version	NM_001002951.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris gap junction protein alpha 1 (GJA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002951.3

ORF Insert Sequence:

ATGGGTGACT GGAGTGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA TTCCACCGCT 
GGAGGGAAGG TGTGGCTGTC TGTCCTTTTC ATTTTCCGAA TTCTGCTACT GGGGACAGCG 
GTTGAGTCGG CCTGGGGTGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA ACAGCCTGGT 
TGTGAAAATG TCTGCTATGA CAAATCCTTC CCAATCTCTC ATGTACGCTT CTGGGTCCTG 
CAGATCATAT TTGTGTCTGT CCCCACACTC TTGTACCTGG CTCATGTGTT CTACGTGATG 
CGCAAGGAAG AGAAACTGAA CAAGAAAGAG GAGGAGCTCA AAGTTGCCCA AACCGATGGT 
GCCAACGTGG ACATGCACTT GAAGCAGATT GAAATAAAGA AGTTCAAGTA TGGAATTGAA 
GAGCACGGCA AGGTGAAAAT GCGAGGGGGC CTGCTGCGAA CCTACATCAT CAGCATCCTG 
TTCAAGTCTG TTTTCGAGGT GGCCTTCTTG CTGATCCAGT GGTACATCTA TGGATTCAGC 
TTGAGTGCCG TTTACACTTG CAAAAGAGAA CCCTGCCCTC ATCAGGTAGA CTGCTTCCTC 
TCTCGCCCCA CGGAGAAAAC CATCTTCATC ATCTTCATGC TGGTCGTGTC CTTGGTGTCT 
CTTGCCTTGA ACATCATCGA ACTCTTCTAT GTCTTCTTCA AGGGTGTTAA GGATCGCGTG 
AAAGGCAAGA GCGATCCTTA CCATGCTACC ACTGGCCCAC TGAGCCCCTC CAAAGACTGT 
GGATCTCCGA AATATGCATA TTTCAATGGC TGCTCCTCAC CCACGGCTCC CCTCTCCCCC 
ATGTCTCCTC CTGGGTACAA GCTGGTTACT GGAGACAGAA ATAATTCTTC CTGCCGCAAT 
TACAACAAAC AAGCAAGTGA GCAAAACTGG GCTAACTACA GTGCAGAACA AAATCGAATG 
GGCCAGGCAG GAAGCACCAT CTCCAACTCC CATGCACAGC CTTTTGATTT CCCTGATGAC 
AACCAGAATT CTAAAAAACT AGCCGCTGGG CATGAACTGC AGCCACTAGC CATTGTGGAC 
CAGCGGCCTT CCAGCAGAGC CAGCAGCCGT GCCAGCAGCC GACCTCGGCC TGACGACCTG 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acetylation mediates Cx43 reduction caused by electrical stimulation.
Journal of molecular and cellular cardiology8754-64(2015 Oct)
Meraviglia V,Azzimato V,Colussi C,Florio MC,Binda A,Panariti A,Qanud K,Suffredini S,Gennaccaro L,Miragoli M,Barbuti A,Lampe PD,Gaetano C,Pramstaller PP,Capogrossi MC,Recchia FA,Pompilio G,Rivolta I,Rossini A

Dynamic alterations of connexin43, matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 during ventricular fibrillation in canine.
Molecular and cellular biochemistry391(1-2)259-66(2014 Jun)
Wang J,Li JS,Liu HZ,Yi SL,Su GY,Zhang Y,Zhong JQ

Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.
Journal of cell science127(Pt 2)455-64(2014 Jan)
Dunn CA,Lampe PD

The involvement of gap junctions in the delayed phase of the protection induced by cardiac pacing in dogs.
Clinical science (London, England : 1979)123(1)39-51(2012 Jul)
G?nczi M,Kov?cs M,Sepr?nyi G,V?gh A

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GJA1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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