BCL2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCL2
Entrez Gene ID	403416
Full Name	BCL2, apoptosis regulator
Synonyms	BCL-2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002949.1	NP_001002949.1	apoptosis regulator Bcl-2

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCL2	NP_000624.2
Pan troglodytes (chimpanzee)	BCL2	XP_001145537.1
Canis lupus familiaris (dog)	BCL2	NP_001002949.1
Xenopus tropicalis (tropical clawed frog)	bcl2	XP_002934442.1
Bos taurus (cattle)	BCL2	NP_001159958.1
Rattus norvegicus (Norway rat)	Bcl2	NP_058689.1
Gallus gallus (chicken)	BCL2	NP_990670.1
Mus musculus (house mouse)	Bcl2	NP_033871.2

Gene Ontology
Bibliography

Related articles in PubMed

Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells.
Tong J, Sun D, Yang C, Wang Y, Sun S, Li Q, Bao J, Liu Y
Research in veterinary science10510-4(2016 Apr)

Bcl-2/caspase 3 mucosal imbalance favors T cell resistance to apoptosis in dogs with inflammatory bowel disease.
Jergens A, Young J, Moore D, Wang C, Hostetter J, Augustine L, Allenspach K, Schmitz S, Mosher C
Veterinary immunology and immunopathology158(3-4)167-74(2014 Apr)

Caspase-3/-8/-9, Bax and Bcl-2 expression in the cerebellum, lymph nodes and leukocytes of dogs naturally infected with canine distemper virus.
Del Puerto HL, Martins AS, Moro L, Milsted A, Alves F, Braz GF, Vasconcelos AC
Genetics and molecular research : GMR9(1)151-61(2010 Jan)

Expression of the anti-apoptotic factors Bcl-2 and survivin in canine vascular tumours.
Murakami M, Sakai H, Kodama A, Mori T, Maruo K, Yanai T, Masegi T
Journal of comparative pathology139(1)1-7(2008 Jul)

Galpha12 stimulates apoptosis in epithelial cells through JNK1-mediated Bcl-2 degradation and up-regulation of IkappaBalpha.
Yanamadala V, Negoro H, Gunaratnam L, Kong T, Denker BM
The Journal of biological chemistry282(33)24352-63(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Serum starvation and TdR methods could achieve sufficient synchronization of CHMm cells. Moreover, the expression of p27, p53 and bcl-2 genes was related to cyclical movements and apoptosis.
Title: Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells.

Mucosal imbalance of Bcl-2/caspase 3 expression favors T cell resistance to apoptosis which may contribute to T cell accumulation and chronic intestinal inflammation.
Title: Bcl-2/caspase 3 mucosal imbalance favors T cell resistance to apoptosis in dogs with inflammatory bowel disease.

The expression of Bax, Bcl-2, and caspase-3, -8 and -9, was evaluated by evaluating mRNA levels in the peripheral blood, lymph nodes and cerebellum of canine distemper virus (CDV)-infected (CDV+) and uninfected (CDV-) dogs.
Title: Caspase-3/-8/-9, Bax and Bcl-2 expression in the cerebellum, lymph nodes and leukocytes of dogs naturally infected with canine distemper virus.

Malignant proliferation in canine hemangiosarcomas is associated with bcl-2 and survivin expression.
Title: Expression of the anti-apoptotic factors Bcl-2 and survivin in canine vascular tumours.

Inducing QLalpha12 led to degradation of the anti-apoptotic protein Bcl-2 (via the proteasome pathway), increased JNK activity, and up-regulated IkappaBalpha protein levels, a potent stimulator of apoptosis.
Title: Galpha12 stimulates apoptosis in epithelial cells through JNK1-mediated Bcl-2 degradation and up-regulation of IkappaBalpha.

The following BCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00148	NM_001002949.1 Latest version!	Canis lupus familiaris BCL2 apoptosis regulator (BCL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCL2 ( NM_001002949.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002949.1 Canis lupus familiaris BCL2 apoptosis regulator (BCL2), mRNA.

Price & Availability↑

CloneID	OCe00148
Clone ID Related Accession (Same CDS sequence)	NM_001002949.1
Accession Version	NM_001002949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Canis lupus familiaris(dog)
Product	apoptosis regulator Bcl-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB154172.1. ##Evidence-Data-START## Transcript exon combination :: AB154172.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGCACG CTGGGCGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAC 
TACAAGCTGT CGCAGAGGGG CTACGAGTGG GACGCGGGAG AGGCGGGCGC CGCGCCCCCG 
GGGGCCGCCC CCGCGCCGGG CATCTTCTCC TCGCAGCCCG GCCGCGCCCC CGCGCCCGCC 
AGGACCTCGC CGCCCCCGCC CCCCGCCGCC CCCGCTGCCG CCGCCGCCGC CGCCGCCGAC 
GCCGCGGGCC CCGCGCCCAG CCCCGTGCCA CCTGTGGTCC ACCTGACCCT GCGCCAGGCC 
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC 
CTGACGCCCT TCACCGCGAG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAT 
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG 
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC 
CTGAACCGGC ATCTGCACAC CTGGATCCAG GACAACGGAG GCTGGGATGC CTTTGTGGAA 
CTGTACGGCC CCACCATGCA GCCTCTGTTT GACTTCTCCT GGCTGTCTCT GAAGGCGCTG 
CTCAGTCTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002949.1
CDS	433..1152
Translation	MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGEAGAAPPGAAPAPGIFSSQPGRAPAPARTSPPPPPAAPAAAAAAAADAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPTMQPLFDFSWLSLKALLSLALVGACITLGAYLGHK

Target ORF information:

RefSeq Version	NM_001002949.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris BCL2 apoptosis regulator (BCL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002949.1

ORF Insert Sequence:

ATGGCGCACG CTGGGCGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAC 
TACAAGCTGT CGCAGAGGGG CTACGAGTGG GACGCGGGAG AGGCGGGCGC CGCGCCCCCG 
GGGGCCGCCC CCGCGCCGGG CATCTTCTCC TCGCAGCCCG GCCGCGCCCC CGCGCCCGCC 
AGGACCTCGC CGCCCCCGCC CCCCGCCGCC CCCGCTGCCG CCGCCGCCGC CGCCGCCGAC 
GCCGCGGGCC CCGCGCCCAG CCCCGTGCCA CCTGTGGTCC ACCTGACCCT GCGCCAGGCC 
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC 
CTGACGCCCT TCACCGCGAG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAT 
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG 
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC 
CTGAACCGGC ATCTGCACAC CTGGATCCAG GACAACGGAG GCTGGGATGC CTTTGTGGAA 
CTGTACGGCC CCACCATGCA GCCTCTGTTT GACTTCTCCT GGCTGTCTCT GAAGGCGCTG 
CTCAGTCTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells.
Research in veterinary science10510-4(2016 Apr)
Tong J,Sun D,Yang C,Wang Y,Sun S,Li Q,Bao J,Liu Y

Bcl-2/caspase 3 mucosal imbalance favors T cell resistance to apoptosis in dogs with inflammatory bowel disease.
Veterinary immunology and immunopathology158(3-4)167-74(2014 Apr)
Jergens A,Young J,Moore D,Wang C,Hostetter J,Augustine L,Allenspach K,Schmitz S,Mosher C

Caspase-3/-8/-9, Bax and Bcl-2 expression in the cerebellum, lymph nodes and leukocytes of dogs naturally infected with canine distemper virus.
Genetics and molecular research : GMR9(1)151-61(2010 Jan)
Del Puerto HL,Martins AS,Moro L,Milsted A,Alves F,Braz GF,Vasconcelos AC

Expression of the anti-apoptotic factors Bcl-2 and survivin in canine vascular tumours.
Journal of comparative pathology139(1)1-7(2008 Jul)
Murakami M,Sakai H,Kodama A,Mori T,Maruo K,Yanai T,Masegi T

Galpha12 stimulates apoptosis in epithelial cells through JNK1-mediated Bcl-2 degradation and up-regulation of IkappaBalpha.
The Journal of biological chemistry282(33)24352-63(2007 Aug)
Yanamadala V,Negoro H,Gunaratnam L,Kong T,Denker BM

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BCL2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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