PDE6B cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE6B
Entrez Gene ID	399653
Full Name	phosphodiesterase 6B
Synonyms	PDBS,PDEB,RCD-1
General protein information	Preferred Names phosphodiesterase 6B Names rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta GMP-PDE beta pde6B gene for cGMP phosphodiesterase beta subunit phosphodiesterase 6B, cGMP-specific, rod, beta (congenital stationary night blindness 3, autosomal dominant) rod cGMP phosphodiesterase beta subunit
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002934.1	NP_001002934.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pde6b	NP_032832.2
Rattus norvegicus (Norway rat)	Pde6b	NP_001099494.1
Danio rerio (zebrafish)	pde6b	XP_685002.1
Bos taurus (cattle)	PDE6B	NP_776843.1
Xenopus tropicalis (tropical clawed frog)	LOC100487945	XP_002935501.2
Canis lupus familiaris (dog)	PDE6B	NP_001002934.1
Homo sapiens (human)	PDE6B	NP_000274.2
	PDE6B	XP_001094732.2
Gallus gallus (chicken)	PDE6B	XP_424876.3

Gene Ontology
Bibliography

Related articles in PubMed

IQCB1 and PDE6B mutations cause similar early onset retinal degenerations in two closely related terrier dog breeds.
Goldstein O, Mezey JG, Schweitzer PA, Boyko AR, Gao C, Bustamante CD, Jordan JA, Aguirre GD, Acland GM
Investigative ophthalmology & visual science54(10)7005-19(2013 Oct)

Generalized progressive retinal atrophy of Sloughi dogs is due to an 8-bp insertion in exon 21 of the PDE6B gene.
Dekomien G, Runte M, G?dde R, Epplen JT
Cytogenetics and cell genetics90(3-4)261-7(2000)

Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene.
Suber ML, Pittler SJ, Qin N, Wright GC, Holcombe V, Lee RH, Craft CM, Lolley RN, Baehr W, Hurwitz RL
Proceedings of the National Academy of Sciences of the United States of America90(9)3968-72(1993 May)

The beta subunit of cyclic GMP phosphodiesterase mRNA is deficient in canine rod-cone dysplasia 1.
Farber DB, Danciger JS, Aguirre G
Neuron9(2)349-56(1992 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Candidate gene evaluation identified a three bases deletion in exon 21 of PDE6B in crd1-affected dogs, and a cytosine insertion in exon 10 of IQCB1 in crd2-affected dogs.
Title: IQCB1 and PDE6B mutations cause similar early onset retinal degenerations in two closely related terrier dog breeds.

The following PDE6B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE6B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00347	NM_001002934.1 Latest version!	Canis lupus familiaris phosphodiesterase 6B (PDE6B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE6B ( NM_001002934.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002934.1 Canis lupus familiaris phosphodiesterase 6B (PDE6B), mRNA.

Price & Availability↑

CloneID	OCe00347
Clone ID Related Accession (Same CDS sequence)	NM_001002934.1
Accession Version	NM_001002934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Canis lupus familiaris(dog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L13262.1. On Sep 1, 2005 this sequence version replaced XM_850451.1. ##Evidence-Data-START## Transcript exon combination :: L13262.1, Z23014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA2240594 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCTCA GTGAGGAGCA GGTGCAGCAC TTCCTAAGCC AGAACCCCGA CTTCACGGAC 
CAGTACTTCG GGAAGACGCT GAGCCCCGAG CATGTGGCTG GCGCCTGCGG GGACGGGCAG 
CCCACGGACT GCGCCAGCTT CCGCGAGCTG TGCCAGGTGG AGGAGAGCGC GGCGCTGTTC 
GAGCTGGTGC AGGACATGCA GGAGAGCGTG AACATGGAGC GCGTGGTGTT CAAGATCCTG 
CGGCGCCTCT GCACCATCCT GCGTGCTGAC CGCTGCAGCC TGTTCATGTA CCGGCAGCGC 
AACGGCGTGG CCGAGCTCGC CACGCGCCTC TTCAGCGTGC AGCCAGGCAG CGCCCTGGAG 
GACTGCCTGG TGCCCCCCGA CTCGGAGATC GTCTTCCCGC TGGATATTGG GGTGGTGGGC 
CACGTGGCTC AGACCAAGAA GATGGTGAAC GTCCAGGACG TGACAGAGTG CCCCCACTTC 
AGCCCCTTCG CCGATGAGCT GACCGGCTAC GAGACCAGGA ACATCCTGGC CACACCCATC 
ATGAACGGCA AGGAGCTGGT GGCTGTCATC ATGGCCCTGA ACAAGCTCGA CGGCCCGTGT 
TTCACGAGTG AGGACGAAGA CGTTTTTCTG AAGTACCTGA ACTTCGGCAC CTTAAACCTG 
AAGATCTACC ACCTGAGCTA CCTTCACAAC TGCGAGACGC GCCGAGGCCA GGTGCTGCTG 
TGGTCGGCCA ACAAAGTGTT TGAGGAGCTG ACGGACATCG AGAGGCAGTT CCACAAGGCC 
TTCTACACGG TGCGGGCCTA CCTGAACTGT GACCGCTACT CCGTGGGCCT CCTGGACATG 
ACCAAGGAGA AGGAATTCTT TGACGTGTGG CCTGTGCTGA TGGGAGAAGC GCAGCCGTAC 
TCGGGCCCAC GCACACCCGA CGGCCGTGAA ATCGTCTTCT ACAAGGTCAT CGACTACATC 
CTCCATGGCA AGGAGGACAT CAAGGTCATC CCCTCGCCCC CCGCCGACCA CTGGGCCTTG 
GCCAGCGGCC TTCCGACCTA CGTGGCAGAA AGCGGCTTTA TCTGTAACAT CATGAACACC 
GCCGCGGACG AGATGTTCAC GTTCCAGGAA GGGCCCCTGG ACGACTCGGG CTGGGTCATC 
AAGAACGTCC TCTCCATGCC CATCGTCAAT AAGAAGGAGG AGATCGTGGG GGTTGCCACG 
TTCTACAACA GGAAGGACGG GAAGCCCTTC GATGAGCAGG ATGAGGTCCT CATGGAGTCC 
CTCACTCAGT TCCTGGGCTG GTCGGTACTG AACACCGACA CCTACGACAA GATGAACAAG 
CTGGAGAACC GCAAGGACAT CGCCCAGGAC ATGGTCCTGT ACCACGTGAG ATGCGACAAG 
GACGAAATCC AGCTGATCCT GCCAACGAGG GAGCGCCTGG GGAAGGAGCC TGCGGACTGT 
GAGGAGGATG AGCTGGGAAT ACTCTTGAAG GAGGTGCTGC CGGGGCCAAG CAAGTTCGAC 
ATCTACGAGT TCCACTTCTC GGACCTGGAG TGCACGGAGC TGGAGCTGGT CAAGTGCGGC 
ATCCAGATGT ACTACGAGCT GGGCGTGGTC CGCAAGTTCC AGATTCCTCA GGAGGTCCTG 
GTGCGCTTCC TGTTCTCCGT GAGCAAAGGG TACCGGAGGA TCACCTACCA CAACTGGCGC 
CACGGCTTCA ACGTGGCCCA GACCATGTTC ACGCTGCTCA CGACGGGCAA GCTGAAGAGC 
TACTACACGG ACCTGGAGGC CTTCGCCATG GTGACGGCGG GCCTGTGCCA CGACATCGAC 
CACCGCGGCA CCAACAACCT GTACCAGATG AAATCCCAGA ACCCCCTGGC CAAGCTCCAC 
GGCTCCTCCA TCCTGGAGCG GCACCACCTG GAGTTCGGGA AGTTCCTGCT CTCCGAGGAG 
ACCCTGAACA TCTACCAGAA CCTGAACCGG CGGCAGCACG AGCACGTGAT CCACCTCATG 
GACATAGCCA TCATCGCCAC CGACCTGGCA CTCTACTTCA AGAAGAGGAC AATGTTCCAG 
AAGATCGTGG ATGAGTCTAA GAACTACGAG GACAGGAAGA GCTGGGTGGA GTACCTGTCC 
CTGGAGACGA CGCGGAAGGA GATAGTCATG GCCATGATGA TGACGGCGTG TGACCTGTCT 
GCCATCACCA AGCCCTGGGA AGTCCAGAGC AAGGTTGCTC TGTTGGTGGC GGCTGAGTTC 
TGGGAACAAG GGGACTTGGA AAGAACAGTT CTGGATCAGC AGCCTATTCC GATGATGGAC 
CGGAACAAGG CAGCCGAGCT CCCCAAACTA CAGGTTGGCT TCATCGACTT CGTGTGCACG 
TTTGTGTACA AGGAGTTTTC CCGTTTCCAC GAAGAGATCC TGCCCATGTT CGACCGACTG 
CAGAACAACA GGAAGGAATG GAAGGCCTTG GCTGATGAGT ACGAGGCCAA GCTGAAGGCC 
CTGGAGGAGG AGAAGCAGCA ACAAGAGGAC AGGACGACAG CCAAGAAAGC GGGCACAGAG 
ATTTGCAACG GTGGCCCCGC ACCCAAGTCT TCCACCTGCT GCATCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002934.1
CDS	17..2587
Translation	MSLSEEQVQHFLSQNPDFTDQYFGKTLSPEHVAGACGDGQPTDCASFRELCQVEESAALFELVQDMQESVNMERVVFKILRRLCTILRADRCSLFMYRQRNGVAELATRLFSVQPGSALEDCLVPPDSEIVFPLDIGVVGHVAQTKKMVNVQDVTECPHFSPFADELTGYETRNILATPIMNGKELVAVIMALNKLDGPCFTSEDEDVFLKYLNFGTLNLKIYHLSYLHNCETRRGQVLLWSANKVFEELTDIERQFHKAFYTVRAYLNCDRYSVGLLDMTKEKEFFDVWPVLMGEAQPYSGPRTPDGREIVFYKVIDYILHGKEDIKVIPSPPADHWALASGLPTYVAESGFICNIMNTAADEMFTFQEGPLDDSGWVIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEQDEVLMESLTQFLGWSVLNTDTYDKMNKLENRKDIAQDMVLYHVRCDKDEIQLILPTRERLGKEPADCEEDELGILLKEVLPGPSKFDIYEFHFSDLECTELELVKCGIQMYYELGVVRKFQIPQEVLVRFLFSVSKGYRRITYHNWRHGFNVAQTMFTLLTTGKLKSYYTDLEAFAMVTAGLCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKFLLSEETLNIYQNLNRRQHEHVIHLMDIAIIATDLALYFKKRTMFQKIVDESKNYEDRKSWVEYLSLETTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLDQQPIPMMDRNKAAELPKLQVGFIDFVCTFVYKEFSRFHEEILPMFDRLQNNRKEWKALADEYEAKLKALEEEKQQQEDRTTAKKAGTEICNGGPAPKSSTCCIL

Target ORF information:

RefSeq Version	NM_001002934.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 6B (PDE6B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002934.1

ORF Insert Sequence:

ATGAGCCTCA GTGAGGAGCA GGTGCAGCAC TTCCTAAGCC AGAACCCCGA CTTCACGGAC 
CAGTACTTCG GGAAGACGCT GAGCCCCGAG CATGTGGCTG GCGCCTGCGG GGACGGGCAG 
CCCACGGACT GCGCCAGCTT CCGCGAGCTG TGCCAGGTGG AGGAGAGCGC GGCGCTGTTC 
GAGCTGGTGC AGGACATGCA GGAGAGCGTG AACATGGAGC GCGTGGTGTT CAAGATCCTG 
CGGCGCCTCT GCACCATCCT GCGTGCTGAC CGCTGCAGCC TGTTCATGTA CCGGCAGCGC 
AACGGCGTGG CCGAGCTCGC CACGCGCCTC TTCAGCGTGC AGCCAGGCAG CGCCCTGGAG 
GACTGCCTGG TGCCCCCCGA CTCGGAGATC GTCTTCCCGC TGGATATTGG GGTGGTGGGC 
CACGTGGCTC AGACCAAGAA GATGGTGAAC GTCCAGGACG TGACAGAGTG CCCCCACTTC 
AGCCCCTTCG CCGATGAGCT GACCGGCTAC GAGACCAGGA ACATCCTGGC CACACCCATC 
ATGAACGGCA AGGAGCTGGT GGCTGTCATC ATGGCCCTGA ACAAGCTCGA CGGCCCGTGT 
TTCACGAGTG AGGACGAAGA CGTTTTTCTG AAGTACCTGA ACTTCGGCAC CTTAAACCTG 
AAGATCTACC ACCTGAGCTA CCTTCACAAC TGCGAGACGC GCCGAGGCCA GGTGCTGCTG 
TGGTCGGCCA ACAAAGTGTT TGAGGAGCTG ACGGACATCG AGAGGCAGTT CCACAAGGCC 
TTCTACACGG TGCGGGCCTA CCTGAACTGT GACCGCTACT CCGTGGGCCT CCTGGACATG 
ACCAAGGAGA AGGAATTCTT TGACGTGTGG CCTGTGCTGA TGGGAGAAGC GCAGCCGTAC 
TCGGGCCCAC GCACACCCGA CGGCCGTGAA ATCGTCTTCT ACAAGGTCAT CGACTACATC 
CTCCATGGCA AGGAGGACAT CAAGGTCATC CCCTCGCCCC CCGCCGACCA CTGGGCCTTG 
GCCAGCGGCC TTCCGACCTA CGTGGCAGAA AGCGGCTTTA TCTGTAACAT CATGAACACC 
GCCGCGGACG AGATGTTCAC GTTCCAGGAA GGGCCCCTGG ACGACTCGGG CTGGGTCATC 
AAGAACGTCC TCTCCATGCC CATCGTCAAT AAGAAGGAGG AGATCGTGGG GGTTGCCACG 
TTCTACAACA GGAAGGACGG GAAGCCCTTC GATGAGCAGG ATGAGGTCCT CATGGAGTCC 
CTCACTCAGT TCCTGGGCTG GTCGGTACTG AACACCGACA CCTACGACAA GATGAACAAG 
CTGGAGAACC GCAAGGACAT CGCCCAGGAC ATGGTCCTGT ACCACGTGAG ATGCGACAAG 
GACGAAATCC AGCTGATCCT GCCAACGAGG GAGCGCCTGG GGAAGGAGCC TGCGGACTGT 
GAGGAGGATG AGCTGGGAAT ACTCTTGAAG GAGGTGCTGC CGGGGCCAAG CAAGTTCGAC 
ATCTACGAGT TCCACTTCTC GGACCTGGAG TGCACGGAGC TGGAGCTGGT CAAGTGCGGC 
ATCCAGATGT ACTACGAGCT GGGCGTGGTC CGCAAGTTCC AGATTCCTCA GGAGGTCCTG 
GTGCGCTTCC TGTTCTCCGT GAGCAAAGGG TACCGGAGGA TCACCTACCA CAACTGGCGC 
CACGGCTTCA ACGTGGCCCA GACCATGTTC ACGCTGCTCA CGACGGGCAA GCTGAAGAGC 
TACTACACGG ACCTGGAGGC CTTCGCCATG GTGACGGCGG GCCTGTGCCA CGACATCGAC 
CACCGCGGCA CCAACAACCT GTACCAGATG AAATCCCAGA ACCCCCTGGC CAAGCTCCAC 
GGCTCCTCCA TCCTGGAGCG GCACCACCTG GAGTTCGGGA AGTTCCTGCT CTCCGAGGAG 
ACCCTGAACA TCTACCAGAA CCTGAACCGG CGGCAGCACG AGCACGTGAT CCACCTCATG 
GACATAGCCA TCATCGCCAC CGACCTGGCA CTCTACTTCA AGAAGAGGAC AATGTTCCAG 
AAGATCGTGG ATGAGTCTAA GAACTACGAG GACAGGAAGA GCTGGGTGGA GTACCTGTCC 
CTGGAGACGA CGCGGAAGGA GATAGTCATG GCCATGATGA TGACGGCGTG TGACCTGTCT 
GCCATCACCA AGCCCTGGGA AGTCCAGAGC AAGGTTGCTC TGTTGGTGGC GGCTGAGTTC 
TGGGAACAAG GGGACTTGGA AAGAACAGTT CTGGATCAGC AGCCTATTCC GATGATGGAC 
CGGAACAAGG CAGCCGAGCT CCCCAAACTA CAGGTTGGCT TCATCGACTT CGTGTGCACG 
TTTGTGTACA AGGAGTTTTC CCGTTTCCAC GAAGAGATCC TGCCCATGTT CGACCGACTG 
CAGAACAACA GGAAGGAATG GAAGGCCTTG GCTGATGAGT ACGAGGCCAA GCTGAAGGCC 
CTGGAGGAGG AGAAGCAGCA ACAAGAGGAC AGGACGACAG CCAAGAAAGC GGGCACAGAG 
ATTTGCAACG GTGGCCCCGC ACCCAAGTCT TCCACCTGCT GCATCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IQCB1 and PDE6B mutations cause similar early onset retinal degenerations in two closely related terrier dog breeds.
Investigative ophthalmology & visual science54(10)7005-19(2013 Oct)
Goldstein O,Mezey JG,Schweitzer PA,Boyko AR,Gao C,Bustamante CD,Jordan JA,Aguirre GD,Acland GM

Generalized progressive retinal atrophy of Sloughi dogs is due to an 8-bp insertion in exon 21 of the PDE6B gene.
Cytogenetics and cell genetics90(3-4)261-7(2000)
Dekomien G,Runte M,G?dde R,Epplen JT

Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene.
Proceedings of the National Academy of Sciences of the United States of America90(9)3968-72(1993 May)
Suber ML,Pittler SJ,Qin N,Wright GC,Holcombe V,Lee RH,Craft CM,Lolley RN,Baehr W,Hurwitz RL

The beta subunit of cyclic GMP phosphodiesterase mRNA is deficient in canine rod-cone dysplasia 1.
Neuron9(2)349-56(1992 Aug)
Farber DB,Danciger JS,Aguirre G

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PDE6B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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