SPG11 cDNA ORF clone, Canis lupus familiaris(dog)

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe44278 XM_014109490.2
Latest version!
Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe44279 XM_014109491.2
Latest version!
Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe28791 XM_003640025.4
Latest version!
Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe28793 XM_005638363.3
Latest version!
Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OCe28791 XM_003640025.3 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe44279 XM_014109491.1 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe28793 XM_005638363.2 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe44278 XM_014109490.1 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe44278
    Clone ID Related Accession (Same CDS sequence) XM_014109490.1 , XM_014109490.2
    Accession Version XM_014109490.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spatacsin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA
    ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT
    CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG
    GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG
    TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG
    AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT
    CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT
    ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA
    CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT
    AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA
    TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG
    ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA
    GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA
    GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG
    TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG
    GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG
    CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC
    TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT
    GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA
    TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC
    CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG
    GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA
    GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC
    CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG
    ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA
    GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA
    AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA
    CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA
    GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC
    CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG
    CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA
    GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG
    AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT
    CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC
    AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA
    CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC
    TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT
    TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG
    GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG
    GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC
    CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG
    GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC
    ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG
    GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT
    GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG
    GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG
    ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA
    AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT
    GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC
    TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC
    GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC
    CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT
    GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT
    CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT
    CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA
    GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC
    CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG
    GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC
    GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG
    AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC
    CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG
    ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC
    TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT
    CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC
    TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC
    ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG
    TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC
    CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA
    AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG
    ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG
    TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC
    CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964965.1
    CDS12..7133
    Translation

    Target ORF information:

    RefSeq Version XM_014109490.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA
    ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT
    CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG
    GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG
    TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG
    AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT
    CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT
    ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA
    CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT
    AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA
    TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG
    ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA
    GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA
    GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG
    TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG
    GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG
    CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC
    TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT
    GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA
    TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC
    CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG
    GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA
    GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC
    CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG
    ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA
    GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA
    AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA
    CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA
    GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC
    CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG
    CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA
    GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG
    AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT
    CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC
    AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA
    CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC
    TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT
    TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG
    GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG
    GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC
    CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG
    GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC
    ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG
    GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT
    GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG
    GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG
    ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA
    AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT
    GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC
    TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC
    GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC
    CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT
    GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT
    CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT
    CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA
    GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC
    CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG
    GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC
    GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG
    AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC
    CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG
    ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC
    TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT
    CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC
    TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC
    ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG
    TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC
    CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA
    AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG
    ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG
    TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC
    CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44278
    Clone ID Related Accession (Same CDS sequence) XM_014109490.1 , XM_014109490.2
    Accession Version XM_014109490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA
    ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT
    CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG
    GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG
    TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG
    AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT
    CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT
    ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA
    CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT
    AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA
    TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG
    ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA
    GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA
    GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG
    TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG
    GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG
    CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC
    TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT
    GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA
    TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC
    CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG
    GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA
    GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC
    CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG
    ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA
    GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA
    AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA
    CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA
    GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC
    CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG
    CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA
    GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG
    AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT
    CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC
    AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA
    CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC
    TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT
    TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG
    GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG
    GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC
    CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG
    GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC
    ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG
    GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT
    GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG
    GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG
    ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA
    AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT
    GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC
    TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC
    GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC
    CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT
    GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT
    CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT
    CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA
    GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC
    CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG
    GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC
    GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG
    AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC
    CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG
    ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC
    TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT
    CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC
    TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC
    ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG
    TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC
    CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA
    AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG
    ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG
    TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC
    CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964965.1
    CDS21..7142
    Translation

    Target ORF information:

    RefSeq Version XM_014109490.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA
    ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT
    CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG
    GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG
    TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG
    AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT
    CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT
    ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA
    CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT
    AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA
    TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG
    ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA
    GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA
    GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG
    TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG
    GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG
    CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC
    TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT
    GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA
    TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC
    CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG
    GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA
    GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC
    CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG
    ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA
    GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA
    AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA
    CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA
    GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC
    CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG
    CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA
    GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG
    AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT
    CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC
    AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA
    CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC
    TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT
    TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG
    GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG
    GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC
    CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG
    GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC
    ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG
    GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT
    GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG
    GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG
    ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA
    AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT
    GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC
    TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC
    GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC
    CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT
    GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT
    CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT
    CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA
    GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC
    CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG
    GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC
    GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG
    AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC
    CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG
    ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC
    TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT
    CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC
    TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC
    ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG
    TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC
    CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA
    AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG
    ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG
    TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC
    CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44279
    Clone ID Related Accession (Same CDS sequence) XM_014109491.1 , XM_014109491.2
    Accession Version XM_014109491.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spatacsin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG
    GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT
    TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC
    TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA
    CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT
    GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG
    TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT
    CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC
    CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC
    AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG
    AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT
    GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG
    ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA
    GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG
    ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT
    CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG
    ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG
    AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT
    TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA
    AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT
    TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA
    CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA
    TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA
    GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT
    TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA
    AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC
    AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG
    CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA
    AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT
    GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT
    TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT
    GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG
    TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA
    TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT
    AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA
    TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG
    AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC
    TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC
    ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT
    TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT
    GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT
    TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT
    AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA
    TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC
    CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG
    AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC
    TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT
    GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG
    AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT
    CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG
    GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA
    GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT
    TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT
    ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT
    CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT
    ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT
    AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG
    CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT
    CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT
    CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC
    AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA
    GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC
    TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT
    CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC
    AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC
    CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC
    ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT
    GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA
    AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA
    TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG
    TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT
    CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT
    AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT
    TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT
    GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA
    CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG
    AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA
    CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA
    TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA
    GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA
    GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA
    GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC
    AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA
    CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG
    TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG
    GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT
    GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA
    AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG
    GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG
    TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG
    GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT
    TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG
    ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA
    GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC
    ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG
    CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA
    GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG
    CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC
    TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC
    TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG
    CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA
    GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC
    GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG
    AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG
    ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC
    CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG
    GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT
    AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA
    TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC
    CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA
    CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA
    AAGGACATGC TAGCAGGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964966.1
    CDS12..7112
    Translation

    Target ORF information:

    RefSeq Version XM_014109491.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG
    GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT
    TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC
    TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA
    CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT
    GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG
    TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT
    CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC
    CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC
    AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG
    AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT
    GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG
    ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA
    GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG
    ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT
    CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG
    ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG
    AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT
    TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA
    AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT
    TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA
    CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA
    TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA
    GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT
    TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA
    AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC
    AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG
    CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA
    AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT
    GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT
    TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT
    GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG
    TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA
    TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT
    AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA
    TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG
    AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC
    TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC
    ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT
    TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT
    GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT
    TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT
    AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA
    TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC
    CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG
    AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC
    TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT
    GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG
    AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT
    CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG
    GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA
    GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT
    TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT
    ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT
    CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT
    ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT
    AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG
    CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT
    CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT
    CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC
    AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA
    GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC
    TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT
    CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC
    AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC
    CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC
    ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT
    GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA
    AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA
    TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG
    TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT
    CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT
    AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT
    TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT
    GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA
    CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG
    AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA
    CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA
    TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA
    GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA
    GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA
    GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC
    AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA
    CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG
    TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG
    GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT
    GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA
    AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG
    GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG
    TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG
    GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT
    TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG
    ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA
    GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC
    ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG
    CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA
    GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG
    CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC
    TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC
    TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG
    CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA
    GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC
    GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG
    AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG
    ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC
    CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG
    GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT
    AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA
    TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC
    CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA
    CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA
    AAGGACATGC TAGCAGGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44279
    Clone ID Related Accession (Same CDS sequence) XM_014109491.1 , XM_014109491.2
    Accession Version XM_014109491.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spatacsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG
    GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT
    TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC
    TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA
    CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT
    GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG
    TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT
    CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC
    CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC
    AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG
    AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT
    GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG
    ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA
    GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG
    ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT
    CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG
    ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG
    AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT
    TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA
    AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT
    TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA
    CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA
    TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA
    GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT
    TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA
    AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC
    AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG
    CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA
    AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT
    GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT
    TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT
    GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG
    TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA
    TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT
    AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA
    TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG
    AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC
    TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC
    ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT
    TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT
    GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT
    TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT
    AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA
    TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC
    CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG
    AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC
    TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT
    GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG
    AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT
    CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG
    GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA
    GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT
    TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT
    ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT
    CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT
    ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT
    AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG
    CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT
    CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT
    CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC
    AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA
    GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC
    TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT
    CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC
    AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC
    CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC
    ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT
    GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA
    AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA
    TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG
    TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT
    CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT
    AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT
    TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT
    GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA
    CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG
    AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA
    CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA
    TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA
    GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA
    GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA
    GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC
    AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA
    CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG
    TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG
    GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT
    GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA
    AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG
    GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG
    TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG
    GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT
    TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG
    ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA
    GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC
    ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG
    CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA
    GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG
    CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC
    TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC
    TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG
    CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA
    GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC
    GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG
    AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG
    ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC
    CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG
    GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT
    AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA
    TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC
    CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA
    CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA
    AAGGACATGC TAGCAGGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964966.1
    CDS21..7121
    Translation

    Target ORF information:

    RefSeq Version XM_014109491.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109491.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG
    GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT
    TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC
    TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA
    CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT
    GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG
    TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT
    CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC
    CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC
    AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG
    AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT
    GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG
    ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA
    GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG
    ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT
    CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG
    ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG
    AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT
    TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA
    AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT
    TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA
    CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA
    TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA
    GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT
    TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA
    AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC
    AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG
    CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA
    AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT
    GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT
    TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT
    GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG
    TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA
    TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT
    AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA
    TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG
    AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC
    TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC
    ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT
    TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT
    GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT
    TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT
    AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA
    TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC
    CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG
    AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC
    TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT
    GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG
    AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT
    CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG
    GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA
    GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT
    TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT
    ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT
    CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT
    ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT
    AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG
    CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT
    CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT
    CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC
    AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA
    GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC
    TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT
    CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC
    AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC
    CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC
    ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT
    GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA
    AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA
    TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG
    TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT
    CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT
    AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT
    TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT
    GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA
    CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG
    AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA
    CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA
    TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA
    GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA
    GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA
    GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC
    AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA
    CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG
    TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG
    GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT
    GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA
    AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG
    GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG
    TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG
    GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT
    TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG
    ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA
    GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC
    ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG
    CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA
    GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG
    CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC
    TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC
    TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG
    CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA
    GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC
    GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG
    AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG
    ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC
    CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG
    GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT
    AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA
    TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC
    CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA
    CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA
    AAGGACATGC TAGCAGGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28791
    Clone ID Related Accession (Same CDS sequence) XM_003640025.3 , XM_003640025.4
    Accession Version XM_003640025.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spatacsin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG
    CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA
    TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG
    CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT
    TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA
    CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT
    CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT
    CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT
    GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT
    CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG
    CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG
    TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT
    TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT
    TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC
    AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG
    CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT
    GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC
    AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA
    TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA
    GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC
    CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC
    CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT
    AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC
    CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT
    GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC
    TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA
    GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT
    ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA
    GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA
    GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA
    CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC
    TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT
    GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG
    GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG
    CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG
    CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT
    AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG
    ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC
    AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG
    TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT
    TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT
    GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG
    CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG
    CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT
    TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA
    CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC
    AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT
    GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT
    TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT
    AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG
    GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG
    TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT
    AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG
    GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA
    ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG
    GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG
    ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA
    TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC
    AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC
    CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC
    CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC
    CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG
    CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC
    CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT
    CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG
    CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG
    CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC
    TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT
    CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG
    GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC
    TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT
    TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA
    AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC
    ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC
    ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA
    GGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640073.2
    CDS12..7337
    Translation

    Target ORF information:

    RefSeq Version XM_003640025.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG
    CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA
    TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG
    CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT
    TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA
    CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT
    CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT
    CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT
    GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT
    CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG
    CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG
    TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT
    TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT
    TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC
    AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG
    CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT
    GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC
    AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA
    TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA
    GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC
    CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC
    CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT
    AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC
    CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT
    GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC
    TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA
    GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT
    ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA
    GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA
    GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA
    CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC
    TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT
    GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG
    GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG
    CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG
    CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT
    AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG
    ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC
    AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG
    TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT
    TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT
    GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG
    CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG
    CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT
    TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA
    CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC
    AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT
    GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT
    TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT
    AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG
    GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG
    TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT
    AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG
    GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA
    ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG
    GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG
    ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA
    TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC
    AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC
    CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC
    CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC
    CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG
    CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC
    CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT
    CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG
    CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG
    CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC
    TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT
    CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG
    GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC
    TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT
    TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA
    AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC
    ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC
    ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA
    GGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28791
    Clone ID Related Accession (Same CDS sequence) XM_003640025.3 , XM_003640025.4
    Accession Version XM_003640025.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG
    CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA
    TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG
    CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT
    TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA
    CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT
    CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT
    CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT
    GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT
    CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG
    CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG
    TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT
    TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT
    TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC
    AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG
    CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT
    GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC
    AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA
    TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA
    GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC
    CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC
    CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT
    AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC
    CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT
    GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC
    TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA
    GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT
    ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA
    GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA
    GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA
    CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC
    TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT
    GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG
    GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG
    CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG
    CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT
    AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG
    ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC
    AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG
    TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT
    TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT
    GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG
    CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG
    CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT
    TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA
    CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC
    AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT
    GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT
    TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT
    AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG
    GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG
    TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT
    AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG
    GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA
    ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG
    GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG
    ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA
    TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC
    AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC
    CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC
    CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC
    CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG
    CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC
    CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT
    CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG
    CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG
    CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC
    TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT
    CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG
    GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC
    TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT
    TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA
    AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC
    ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC
    ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA
    GGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640073.2
    CDS21..7346
    Translation

    Target ORF information:

    RefSeq Version XM_003640025.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640025.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
    ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG
    GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC
    AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT
    GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG
    CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA
    GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC
    ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT
    AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA
    GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC
    AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG
    ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC
    CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA
    CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT
    GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT
    CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT
    GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG
    CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA
    TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA
    CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA
    GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA
    AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG
    GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG
    TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC
    CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT
    TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT
    GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG
    CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA
    TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA
    AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC
    CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA
    ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC
    ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA
    GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT
    ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA
    CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG
    ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA
    TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC
    TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT
    TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA
    GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG
    GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA
    TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA
    CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA
    GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA
    GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT
    GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT
    CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG
    CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA
    TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG
    CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT
    TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA
    CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT
    CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT
    CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT
    GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT
    CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG
    CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG
    TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT
    TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT
    TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC
    AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG
    CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT
    GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC
    AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA
    TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA
    GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC
    CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC
    CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT
    AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC
    CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT
    GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC
    TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA
    GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT
    ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA
    GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA
    GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA
    CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC
    TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT
    GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG
    GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG
    CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG
    CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT
    AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG
    ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC
    AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG
    TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT
    TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT
    GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG
    CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG
    CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT
    TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA
    CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC
    AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT
    GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT
    TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT
    AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG
    GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG
    TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT
    AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG
    GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA
    ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG
    GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG
    ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA
    TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC
    AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC
    CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC
    CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC
    CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG
    CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC
    CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT
    CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG
    CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG
    CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC
    TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT
    CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG
    GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC
    TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT
    TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA
    AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC
    ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC
    ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA
    GGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28793
    Clone ID Related Accession (Same CDS sequence) XM_005638363.2 , XM_005638363.3
    Accession Version XM_005638363.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spatacsin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
    CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG
    AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG
    GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC
    AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT
    GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA
    AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC
    CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG
    ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA
    CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG
    AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC
    GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA
    CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA
    CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA
    CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG
    CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC
    AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA
    GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG
    AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA
    CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA
    GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT
    TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT
    GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA
    AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT
    CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC
    AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA
    CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC
    ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG
    AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG
    GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT
    ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC
    AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG
    AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA
    GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT
    CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA
    AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC
    AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG
    ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC
    CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT
    GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC
    AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT
    ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG
    CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC
    CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA
    CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG
    ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC
    TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT
    AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA
    AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG
    GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG
    AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA
    TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA
    TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC
    CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA
    CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC
    AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT
    CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA
    CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT
    TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA
    CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG
    AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT
    AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT
    TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA
    GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT
    CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG
    CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC
    CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC
    AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA
    CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT
    ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA
    CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA
    AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT
    CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG
    GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC
    ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG
    ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG
    TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA
    TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA
    TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC
    CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT
    GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA
    GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG
    ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC
    CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC
    TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT
    TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG
    GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG
    GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG
    CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG
    TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC
    TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC
    ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC
    ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT
    GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC
    TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC
    AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG
    ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG
    CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG
    CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG
    AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC
    CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT
    GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA
    GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT
    AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT
    GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT
    GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638420.1
    CDS187..6543
    Translation

    Target ORF information:

    RefSeq Version XM_005638363.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638363.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
    CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG
    AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG
    GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC
    AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT
    GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA
    AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC
    CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG
    ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA
    CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG
    AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC
    GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA
    CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA
    CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA
    CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG
    CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC
    AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA
    GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG
    AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA
    CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA
    GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT
    TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT
    GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA
    AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT
    CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC
    AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA
    CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC
    ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG
    AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG
    GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT
    ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC
    AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG
    AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA
    GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT
    CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA
    AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC
    AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG
    ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC
    CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT
    GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC
    AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT
    ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG
    CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC
    CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA
    CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG
    ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC
    TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT
    AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA
    AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG
    GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG
    AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA
    TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA
    TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC
    CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA
    CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC
    AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT
    CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA
    CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT
    TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA
    CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG
    AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT
    AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT
    TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA
    GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT
    CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG
    CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC
    CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC
    AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA
    CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT
    ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA
    CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA
    AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT
    CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG
    GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC
    ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG
    ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG
    TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA
    TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA
    TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC
    CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT
    GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA
    GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG
    ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC
    CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC
    TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT
    TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG
    GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG
    GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG
    CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG
    TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC
    TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC
    ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC
    ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT
    GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC
    TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC
    AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG
    ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG
    CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG
    CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG
    AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC
    CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT
    GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA
    GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT
    AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT
    GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT
    GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28793
    Clone ID Related Accession (Same CDS sequence) XM_005638363.2 , XM_005638363.3
    Accession Version XM_005638363.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spatacsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
    CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG
    AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG
    GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC
    AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT
    GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA
    AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC
    CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG
    ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA
    CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG
    AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC
    GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA
    CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA
    CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA
    CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG
    CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC
    AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA
    GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG
    AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA
    CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA
    GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT
    TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT
    GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA
    AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT
    CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC
    AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA
    CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC
    ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG
    AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG
    GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT
    ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC
    AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG
    AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA
    GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT
    CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA
    AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC
    AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG
    ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC
    CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT
    GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC
    AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT
    ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG
    CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC
    CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA
    CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG
    ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC
    TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT
    AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA
    AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG
    GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG
    AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA
    TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA
    TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC
    CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA
    CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC
    AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT
    CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA
    CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT
    TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA
    CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG
    AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT
    AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT
    TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA
    GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT
    CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG
    CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC
    CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC
    AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA
    CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT
    ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA
    CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA
    AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT
    CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG
    GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC
    ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG
    ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG
    TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA
    TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA
    TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC
    CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT
    GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA
    GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG
    ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC
    CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC
    TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT
    TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG
    GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG
    GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG
    CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG
    TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC
    TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC
    ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC
    ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT
    GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC
    TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC
    AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG
    ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG
    CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG
    CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG
    AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC
    CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT
    GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA
    GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT
    AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT
    GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT
    GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638420.1
    CDS238..6594
    Translation

    Target ORF information:

    RefSeq Version XM_005638363.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638363.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
    CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG
    AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG
    GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC
    AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT
    GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA
    AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC
    CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG
    ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA
    CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG
    AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC
    GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA
    CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA
    CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA
    CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG
    CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC
    AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA
    GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG
    AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA
    CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA
    GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT
    TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT
    GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA
    AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT
    CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC
    AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA
    CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC
    ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG
    AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG
    GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT
    ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC
    AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG
    AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA
    GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT
    CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA
    AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC
    AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG
    ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC
    CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT
    GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC
    AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT
    ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG
    CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC
    CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA
    CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG
    ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC
    TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT
    AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA
    AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG
    GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG
    AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA
    TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA
    TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC
    CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA
    CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC
    AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT
    CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA
    CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT
    TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA
    CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG
    AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT
    AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT
    TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA
    GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT
    CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG
    CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC
    CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC
    AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA
    CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT
    ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA
    CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA
    AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT
    CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG
    GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC
    ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG
    ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG
    TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA
    TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA
    TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC
    CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT
    GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA
    GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG
    ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC
    CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC
    TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT
    TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG
    GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG
    GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG
    CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG
    TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC
    TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC
    ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC
    ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT
    GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC
    TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC
    AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG
    ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG
    CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG
    CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG
    AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC
    CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT
    GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA
    GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT
    AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT
    GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT
    GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.