NHSL1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following NHSL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHSL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe00875 XM_005615453.3
Latest version!
Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe00877 XM_005615454.3
Latest version!
Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe00878 XM_005615455.3
Latest version!
Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe285859 XM_022417475.1
Latest version!
Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe285859 XM_022417481.1
Latest version!
Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe34437 XM_014112351.2
Latest version!
Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OCe00877 XM_005615454.2 Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe00875 XM_005615453.2 Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe00878 XM_005615455.2 Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe34437 XM_014112351.1 Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe00875
    Clone ID Related Accession (Same CDS sequence) XM_005615453.3 , XM_005615453.2
    Accession Version XM_005615453.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGGGG AGAATTTCGA TCGCCAGGCC AGTCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTACAGG AGTCCCTGAC
    AACATACAGA AGGAGCTAGC ATCAGTCACT GGCCAAGATG ATGGTGGTGG TCACTCGTTG
    TACACCCCAG ACCACTGCTC CACACTGGGA AGGCTTGATA GCTATTGCTC TGCTGGGCAG
    CGCTCAGAAA CCAGGGACTC CAGCTGTCAG ACTGAGGAAG TGAAAGTTGT ACCCCCTTCA
    ATGAGAAGAA TCAGGGCACA GAAGGGGCAA GGCATTGCTG CCCAGATGAG CCACTTCTCA
    GGCTCCTCCG GGAACATGTC TGTGTTGAGT GATTCTGCAG GTATTGTGTT CCCTTCTCGC
    CTCAACAATG ATGCTGGCTT CCACAGTCTC CCACGTTCTG GAGCGAGGGT GAGTGTTCAG
    TCCCTCGAGT CACGATTGGG TGCCCTGAGC CCTGCAGAAG ACCTGGATGG CACTTATCCC
    TACCAGAGTG GTCACCCACA AGTAGATGAA AATTCAGGAC ATTTGGGAGG TGCCTCAAGG
    ACTGGAATGC TTTTGAGACC CAAGTCCCAG GAGCTGAGGC ATTTTGAGAA TGTGATCAGC
    CCAGCGTGTG TGGTCTCTCC TCATGCCACT TACTCCACCA GCATCATCCC AAATGCCACA
    CTCTCTTCCT CTTCAGAGAT TATTATTATT CACACTGCTC AGAGTTCAGG ACAGCTGGAC
    AGTAAAATCA CCAGCTCCTC TTCATACTCA AAGATAAAAT CTAGAGACCA CCTCCTCTCC
    AGGCATACTA GTAAAGATGA TCATCAGTCT CCCAGTGGCA ACTGGACAGA GGGTCACCCC
    AGCATTCTTT CACAGGCCTT AGATCCCCAC ACCTCCAGTG CAACCATGCT ATTGTCCCTC
    TGTGATTCAG TGGTTTCTCT AAATACTTCA GCAAATCGGG AGAATGGGTC CCAAGCCATG
    AGCTATAACT GTAAAAACAA CCTGAGTTCT CCGGCCCACG CCCAGGATGT GGATGGCAAG
    AGTGAGTCCA GTTATTCAGG AGGCACGGGG CCCAGCAGCT CGGAGCCCTG GGAATATAGT
    GCCCCCGGTA ACGGGCAAGC ATGCCCACTG AAGGCTCATT TGACAACGCC TGGTTACTCC
    ACTCCCGGAA GTAATGTGAG CAGCTGCAGC TTGGACCAAA CATCCACCAA AGATGATGCC
    AGATCCCTGT ATTCAGAGGA GCATGATGGC TACTACACAT CTGTGCACCC CGATCCGGGA
    CAGGGATCTG GAAACCAGTA CAATAGTGAT GGCTTTGGGA ACCCCAGGCA CAGTGTGGTC
    AATGTTTTTG ATGGAAGAGC TCAGAAAAAC CAAGGGGACC GGTCACATTA CCATGACAAG
    TCCCTTTCTC GAAACATCTC TTTGAAGAAA GCGAAGAAGC CGCCTCTGCC ACCCTCTCGG
    ACTGACTCCC TGCGCAGGAT TCCCAAGAAG GGTGTCCAGT CCAATGGGCA GGCGCTGAAC
    GAGAGCCTGA TCGCCTCGCT GCAGCACTCG CTGCAGCTGA ACCTCCCGGG CAAGGGCAGC
    AGCTCGCCCT CCCAGAGCCC CTGCAGCGAC TTTGAAGAGC CCTGGCTGCC TCGCTCCCGC
    AGCCAGAGCA CGGTGAGTGC GGGCAGCAGC ATGACCTCGG CCACCACCCC CAACGTCTAC
    TCCCTGTGCG GGGCCACGCC GTCGCAGAGT GACACGAGCA GCGTCAAGTC TGAGTACACA
    GACCCTTGGG GTTACTACAT TGACTACACA GGCATGCAGG AAGACCCCGG GCCCCCTGGC
    GGGGGCTGCT CAGCCAGCAG TGGGGCGCCG GCTGGAAATG GGCCAGTCCA CCACGTCCAG
    GAGGGATCCA GGACCCAAGT GCCTGGTGGC TCAGTCAAAC CAAAGATCAC GTCGCCAGAG
    AAGTCCCACA GAGTCACTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA
    GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAATGG GAAGGGGAAG
    ACCAAGCCCA AGGTGCCAGA AAGGAAGTCC TCTCTGATAT GTTCAGTATC CATCTCCTCA
    TCGTCCACGT CTCTGTCTTC CAATACTTCC ACGGAAGGAA GCGGGACAAT GAAGAAGCTG
    GACTCGGTGC TGGCCTCCCC CACTGCGGGT GCTTCTCTCC TTTCTCTCCC CTCACCGTGT
    GCTGCTGACA AGTCTCCTTT GCTTCCTCCT CCCCCACCTT TAGTAGATTC CCCTGACGGC
    TCTCTGCCTC AGTCTCCCCT CTTCCCCCCT CCACCACCGG AAGTTCTCAC TCCCTTCTCT
    GCCCCTGCCA GCGGGTGCCT CCCTCCTTCC CCCAGGGCAC TCAGCCCCCT TACTCTGGGT
    GCATCTGCTT CCCTGCCACT GCCCCCCGCT GCCCTGCTCT CCTCAGCTCC CCCGCCTGCC
    CCACCCCTGG ACCCTACATT GATGAAAGAT GCCAGGCCTT CTTTCAAAAA ACCTGGCCAG
    CCCGAGTGCT CCCGGGAGGG CTTCCGGCAG CCTTGTAACA AGGAGGAAGG CGGTAGGCCC
    CCCATGCCCC TGATAACCAC TGAAACGTTG CAGATGGTAC AGTTGAGGCC CGTGAAAAAG
    AACTCAGGAA CCGAGCAAGC ACTGTTATAT GAACAAGTAT CTCAGGAAAA ACTAACTCCG
    GTCGTCCCCC AGTATCATTT AAAGCCATCT GCTTTCTTGA AATCCCGAAA TAGCATAAAT
    GAAATGGAGA GTGAAAGCCA GCCTGCCTCT GTGACAAGCT CGCCGCCGCC TCCTGCCAAG
    AGCTCGAGTC AGGGTCACCG GGACGGTGCA GCCGAGCATG GTCCCCCGAG CCGCAGCCCG
    GGCGGTGCCA GGGAGGCAGA GGCTGGGCCC GGCGCCCGGC CCGCCCCGCT CCAGGAGCCC
    CCCGGCCCTT CACCCAGCAG GAAGCCACCC CCCATTTCCA AGAAGCCCAA ACTGTTTCTC
    GTGGTGCCAC CTCCGCAGAG AGATCTCACA GCGGAGCCCG CGGAGCCCGC GAGCGGAGCA
    TCACCCGGCC CCAGGCGGGG CGAGGCAGCC GAGAGTGGTA GAGCCAGCGC TGGTCCTGAT
    GAGCCGGGCT CTGGCAGCGC CGTGCTCGGG GGAGGAGCCG CAGGAGCCGC GGCCCCGGCC
    CCGGGTGGAG TGAAAGCCGA CGTCCCCATG GTGCAGCCCG ACGTGCAGCC AGCCCCCAAG
    CAGGAGGAGC CAGGTGCCGG GACCAGTGCC GACGGCGCAG GCGACGTGGA GAGCCGCCTG
    CCTCTCCAGG ACGGACGGCC TGGGCTGCCA GAGCCTGACG CAGCCGGCTC TTCCTCGGAG
    GCCTGTGACT TCCCCAGGGA AGAAGGGAGT GATGAGGTGA TGACCCCCAG TAGACCCCGG
    ACCACAGAAG ACCTTTTTGC AGCTATTCAC AGATCCAAGA GGAAAGTCCT GGGCCGGAAG
    GATTCAGATG ACGATCACTC CCGAAATCAT TCTCCCTCCC CTCCGGTGAC GCCCACCGGT
    GCGGCCCCCA GCCTGGCTTC CCCCAAGCAA GTGGGGTCAA TTCAGAGAAG TGTCCGGAAG
    AGCAGCACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AAAAGGGGAG CCGCTCGGAC
    ACCAGCGCCC GCATGTCTGC AGCTGAGATG CTAAAGAACA CAGACCCTAG ATTCCAGAGG
    TCAAGGTCAG AGCCTTCGCC AGATACTCCC GAGAGCCCGT CAAGCTGCTC CCCCAGCAAG
    AACAGAAGGG CCCAGGAGGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTCTCTGGCC CCCGGTACGG GCGCAGTCGA ACTCCACCTT CTGCAGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGAATCCA GAGCAGTCCC ATGACTGTCA TCTCAGAAGG CGAAGGGGAG
    GCCATGGAGC CTGTAGATAG CCGGGCTCAC GGGGCCCTGG GCACCGCCAG GGGGTGTTCC
    ATGAATGGAC TAGGTGGGGA GCCCACCACC CCCCTGCAGG CACAGGGTCC TGGCCCTGCT
    GATGGGATAG TCAGCGCAGA GGGCACACAT GGAACAGAAC AACGTGGAGG TCCTCAGAGG
    GAAGAGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615510.1
    CDS682..5553
    Translation

    Target ORF information:

    RefSeq Version XM_005615453.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615453.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGGGG AGAATTTCGA TCGCCAGGCC AGTCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTACAGG AGTCCCTGAC
    AACATACAGA AGGAGCTAGC ATCAGTCACT GGCCAAGATG ATGGTGGTGG TCACTCGTTG
    TACACCCCAG ACCACTGCTC CACACTGGGA AGGCTTGATA GCTATTGCTC TGCTGGGCAG
    CGCTCAGAAA CCAGGGACTC CAGCTGTCAG ACTGAGGAAG TGAAAGTTGT ACCCCCTTCA
    ATGAGAAGAA TCAGGGCACA GAAGGGGCAA GGCATTGCTG CCCAGATGAG CCACTTCTCA
    GGCTCCTCCG GGAACATGTC TGTGTTGAGT GATTCTGCAG GTATTGTGTT CCCTTCTCGC
    CTCAACAATG ATGCTGGCTT CCACAGTCTC CCACGTTCTG GAGCGAGGGT GAGTGTTCAG
    TCCCTCGAGT CACGATTGGG TGCCCTGAGC CCTGCAGAAG ACCTGGATGG CACTTATCCC
    TACCAGAGTG GTCACCCACA AGTAGATGAA AATTCAGGAC ATTTGGGAGG TGCCTCAAGG
    ACTGGAATGC TTTTGAGACC CAAGTCCCAG GAGCTGAGGC ATTTTGAGAA TGTGATCAGC
    CCAGCGTGTG TGGTCTCTCC TCATGCCACT TACTCCACCA GCATCATCCC AAATGCCACA
    CTCTCTTCCT CTTCAGAGAT TATTATTATT CACACTGCTC AGAGTTCAGG ACAGCTGGAC
    AGTAAAATCA CCAGCTCCTC TTCATACTCA AAGATAAAAT CTAGAGACCA CCTCCTCTCC
    AGGCATACTA GTAAAGATGA TCATCAGTCT CCCAGTGGCA ACTGGACAGA GGGTCACCCC
    AGCATTCTTT CACAGGCCTT AGATCCCCAC ACCTCCAGTG CAACCATGCT ATTGTCCCTC
    TGTGATTCAG TGGTTTCTCT AAATACTTCA GCAAATCGGG AGAATGGGTC CCAAGCCATG
    AGCTATAACT GTAAAAACAA CCTGAGTTCT CCGGCCCACG CCCAGGATGT GGATGGCAAG
    AGTGAGTCCA GTTATTCAGG AGGCACGGGG CCCAGCAGCT CGGAGCCCTG GGAATATAGT
    GCCCCCGGTA ACGGGCAAGC ATGCCCACTG AAGGCTCATT TGACAACGCC TGGTTACTCC
    ACTCCCGGAA GTAATGTGAG CAGCTGCAGC TTGGACCAAA CATCCACCAA AGATGATGCC
    AGATCCCTGT ATTCAGAGGA GCATGATGGC TACTACACAT CTGTGCACCC CGATCCGGGA
    CAGGGATCTG GAAACCAGTA CAATAGTGAT GGCTTTGGGA ACCCCAGGCA CAGTGTGGTC
    AATGTTTTTG ATGGAAGAGC TCAGAAAAAC CAAGGGGACC GGTCACATTA CCATGACAAG
    TCCCTTTCTC GAAACATCTC TTTGAAGAAA GCGAAGAAGC CGCCTCTGCC ACCCTCTCGG
    ACTGACTCCC TGCGCAGGAT TCCCAAGAAG GGTGTCCAGT CCAATGGGCA GGCGCTGAAC
    GAGAGCCTGA TCGCCTCGCT GCAGCACTCG CTGCAGCTGA ACCTCCCGGG CAAGGGCAGC
    AGCTCGCCCT CCCAGAGCCC CTGCAGCGAC TTTGAAGAGC CCTGGCTGCC TCGCTCCCGC
    AGCCAGAGCA CGGTGAGTGC GGGCAGCAGC ATGACCTCGG CCACCACCCC CAACGTCTAC
    TCCCTGTGCG GGGCCACGCC GTCGCAGAGT GACACGAGCA GCGTCAAGTC TGAGTACACA
    GACCCTTGGG GTTACTACAT TGACTACACA GGCATGCAGG AAGACCCCGG GCCCCCTGGC
    GGGGGCTGCT CAGCCAGCAG TGGGGCGCCG GCTGGAAATG GGCCAGTCCA CCACGTCCAG
    GAGGGATCCA GGACCCAAGT GCCTGGTGGC TCAGTCAAAC CAAAGATCAC GTCGCCAGAG
    AAGTCCCACA GAGTCACTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA
    GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAATGG GAAGGGGAAG
    ACCAAGCCCA AGGTGCCAGA AAGGAAGTCC TCTCTGATAT GTTCAGTATC CATCTCCTCA
    TCGTCCACGT CTCTGTCTTC CAATACTTCC ACGGAAGGAA GCGGGACAAT GAAGAAGCTG
    GACTCGGTGC TGGCCTCCCC CACTGCGGGT GCTTCTCTCC TTTCTCTCCC CTCACCGTGT
    GCTGCTGACA AGTCTCCTTT GCTTCCTCCT CCCCCACCTT TAGTAGATTC CCCTGACGGC
    TCTCTGCCTC AGTCTCCCCT CTTCCCCCCT CCACCACCGG AAGTTCTCAC TCCCTTCTCT
    GCCCCTGCCA GCGGGTGCCT CCCTCCTTCC CCCAGGGCAC TCAGCCCCCT TACTCTGGGT
    GCATCTGCTT CCCTGCCACT GCCCCCCGCT GCCCTGCTCT CCTCAGCTCC CCCGCCTGCC
    CCACCCCTGG ACCCTACATT GATGAAAGAT GCCAGGCCTT CTTTCAAAAA ACCTGGCCAG
    CCCGAGTGCT CCCGGGAGGG CTTCCGGCAG CCTTGTAACA AGGAGGAAGG CGGTAGGCCC
    CCCATGCCCC TGATAACCAC TGAAACGTTG CAGATGGTAC AGTTGAGGCC CGTGAAAAAG
    AACTCAGGAA CCGAGCAAGC ACTGTTATAT GAACAAGTAT CTCAGGAAAA ACTAACTCCG
    GTCGTCCCCC AGTATCATTT AAAGCCATCT GCTTTCTTGA AATCCCGAAA TAGCATAAAT
    GAAATGGAGA GTGAAAGCCA GCCTGCCTCT GTGACAAGCT CGCCGCCGCC TCCTGCCAAG
    AGCTCGAGTC AGGGTCACCG GGACGGTGCA GCCGAGCATG GTCCCCCGAG CCGCAGCCCG
    GGCGGTGCCA GGGAGGCAGA GGCTGGGCCC GGCGCCCGGC CCGCCCCGCT CCAGGAGCCC
    CCCGGCCCTT CACCCAGCAG GAAGCCACCC CCCATTTCCA AGAAGCCCAA ACTGTTTCTC
    GTGGTGCCAC CTCCGCAGAG AGATCTCACA GCGGAGCCCG CGGAGCCCGC GAGCGGAGCA
    TCACCCGGCC CCAGGCGGGG CGAGGCAGCC GAGAGTGGTA GAGCCAGCGC TGGTCCTGAT
    GAGCCGGGCT CTGGCAGCGC CGTGCTCGGG GGAGGAGCCG CAGGAGCCGC GGCCCCGGCC
    CCGGGTGGAG TGAAAGCCGA CGTCCCCATG GTGCAGCCCG ACGTGCAGCC AGCCCCCAAG
    CAGGAGGAGC CAGGTGCCGG GACCAGTGCC GACGGCGCAG GCGACGTGGA GAGCCGCCTG
    CCTCTCCAGG ACGGACGGCC TGGGCTGCCA GAGCCTGACG CAGCCGGCTC TTCCTCGGAG
    GCCTGTGACT TCCCCAGGGA AGAAGGGAGT GATGAGGTGA TGACCCCCAG TAGACCCCGG
    ACCACAGAAG ACCTTTTTGC AGCTATTCAC AGATCCAAGA GGAAAGTCCT GGGCCGGAAG
    GATTCAGATG ACGATCACTC CCGAAATCAT TCTCCCTCCC CTCCGGTGAC GCCCACCGGT
    GCGGCCCCCA GCCTGGCTTC CCCCAAGCAA GTGGGGTCAA TTCAGAGAAG TGTCCGGAAG
    AGCAGCACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AAAAGGGGAG CCGCTCGGAC
    ACCAGCGCCC GCATGTCTGC AGCTGAGATG CTAAAGAACA CAGACCCTAG ATTCCAGAGG
    TCAAGGTCAG AGCCTTCGCC AGATACTCCC GAGAGCCCGT CAAGCTGCTC CCCCAGCAAG
    AACAGAAGGG CCCAGGAGGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTCTCTGGCC CCCGGTACGG GCGCAGTCGA ACTCCACCTT CTGCAGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGAATCCA GAGCAGTCCC ATGACTGTCA TCTCAGAAGG CGAAGGGGAG
    GCCATGGAGC CTGTAGATAG CCGGGCTCAC GGGGCCCTGG GCACCGCCAG GGGGTGTTCC
    ATGAATGGAC TAGGTGGGGA GCCCACCACC CCCCTGCAGG CACAGGGTCC TGGCCCTGCT
    GATGGGATAG TCAGCGCAGA GGGCACACAT GGAACAGAAC AACGTGGAGG TCCTCAGAGG
    GAAGAGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00875
    Clone ID Related Accession (Same CDS sequence) XM_005615453.3 , XM_005615453.2
    Accession Version XM_005615453.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGGGG AGAATTTCGA TCGCCAGGCC AGTCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTACAGG AGTCCCTGAC
    AACATACAGA AGGAGCTAGC ATCAGTCACT GGCCAAGATG ATGGTGGTGG TCACTCGTTG
    TACACCCCAG ACCACTGCTC CACACTGGGA AGGCTTGATA GCTATTGCTC TGCTGGGCAG
    CGCTCAGAAA CCAGGGACTC CAGCTGTCAG ACTGAGGAAG TGAAAGTTGT ACCCCCTTCA
    ATGAGAAGAA TCAGGGCACA GAAGGGGCAA GGCATTGCTG CCCAGATGAG CCACTTCTCA
    GGCTCCTCCG GGAACATGTC TGTGTTGAGT GATTCTGCAG GTATTGTGTT CCCTTCTCGC
    CTCAACAATG ATGCTGGCTT CCACAGTCTC CCACGTTCTG GAGCGAGGGT GAGTGTTCAG
    TCCCTCGAGT CACGATTGGG TGCCCTGAGC CCTGCAGAAG ACCTGGATGG CACTTATCCC
    TACCAGAGTG GTCACCCACA AGTAGATGAA AATTCAGGAC ATTTGGGAGG TGCCTCAAGG
    ACTGGAATGC TTTTGAGACC CAAGTCCCAG GAGCTGAGGC ATTTTGAGAA TGTGATCAGC
    CCAGCGTGTG TGGTCTCTCC TCATGCCACT TACTCCACCA GCATCATCCC AAATGCCACA
    CTCTCTTCCT CTTCAGAGAT TATTATTATT CACACTGCTC AGAGTTCAGG ACAGCTGGAC
    AGTAAAATCA CCAGCTCCTC TTCATACTCA AAGATAAAAT CTAGAGACCA CCTCCTCTCC
    AGGCATACTA GTAAAGATGA TCATCAGTCT CCCAGTGGCA ACTGGACAGA GGGTCACCCC
    AGCATTCTTT CACAGGCCTT AGATCCCCAC ACCTCCAGTG CAACCATGCT ATTGTCCCTC
    TGTGATTCAG TGGTTTCTCT AAATACTTCA GCAAATCGGG AGAATGGGTC CCAAGCCATG
    AGCTATAACT GTAAAAACAA CCTGAGTTCT CCGGCCCACG CCCAGGATGT GGATGGCAAG
    AGTGAGTCCA GTTATTCAGG AGGCACGGGG CCCAGCAGCT CGGAGCCCTG GGAATATAGT
    GCCCCCGGTA ACGGGCAAGC ATGCCCACTG AAGGCTCATT TGACAACGCC TGGTTACTCC
    ACTCCCGGAA GTAATGTGAG CAGCTGCAGC TTGGACCAAA CATCCACCAA AGATGATGCC
    AGATCCCTGT ATTCAGAGGA GCATGATGGC TACTACACAT CTGTGCACCC CGATCCGGGA
    CAGGGATCTG GAAACCAGTA CAATAGTGAT GGCTTTGGGA ACCCCAGGCA CAGTGTGGTC
    AATGTTTTTG ATGGAAGAGC TCAGAAAAAC CAAGGGGACC GGTCACATTA CCATGACAAG
    TCCCTTTCTC GAAACATCTC TTTGAAGAAA GCGAAGAAGC CGCCTCTGCC ACCCTCTCGG
    ACTGACTCCC TGCGCAGGAT TCCCAAGAAG GGTGTCCAGT CCAATGGGCA GGCGCTGAAC
    GAGAGCCTGA TCGCCTCGCT GCAGCACTCG CTGCAGCTGA ACCTCCCGGG CAAGGGCAGC
    AGCTCGCCCT CCCAGAGCCC CTGCAGCGAC TTTGAAGAGC CCTGGCTGCC TCGCTCCCGC
    AGCCAGAGCA CGGTGAGTGC GGGCAGCAGC ATGACCTCGG CCACCACCCC CAACGTCTAC
    TCCCTGTGCG GGGCCACGCC GTCGCAGAGT GACACGAGCA GCGTCAAGTC TGAGTACACA
    GACCCTTGGG GTTACTACAT TGACTACACA GGCATGCAGG AAGACCCCGG GCCCCCTGGC
    GGGGGCTGCT CAGCCAGCAG TGGGGCGCCG GCTGGAAATG GGCCAGTCCA CCACGTCCAG
    GAGGGATCCA GGACCCAAGT GCCTGGTGGC TCAGTCAAAC CAAAGATCAC GTCGCCAGAG
    AAGTCCCACA GAGTCACTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA
    GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAATGG GAAGGGGAAG
    ACCAAGCCCA AGGTGCCAGA AAGGAAGTCC TCTCTGATAT GTTCAGTATC CATCTCCTCA
    TCGTCCACGT CTCTGTCTTC CAATACTTCC ACGGAAGGAA GCGGGACAAT GAAGAAGCTG
    GACTCGGTGC TGGCCTCCCC CACTGCGGGT GCTTCTCTCC TTTCTCTCCC CTCACCGTGT
    GCTGCTGACA AGTCTCCTTT GCTTCCTCCT CCCCCACCTT TAGTAGATTC CCCTGACGGC
    TCTCTGCCTC AGTCTCCCCT CTTCCCCCCT CCACCACCGG AAGTTCTCAC TCCCTTCTCT
    GCCCCTGCCA GCGGGTGCCT CCCTCCTTCC CCCAGGGCAC TCAGCCCCCT TACTCTGGGT
    GCATCTGCTT CCCTGCCACT GCCCCCCGCT GCCCTGCTCT CCTCAGCTCC CCCGCCTGCC
    CCACCCCTGG ACCCTACATT GATGAAAGAT GCCAGGCCTT CTTTCAAAAA ACCTGGCCAG
    CCCGAGTGCT CCCGGGAGGG CTTCCGGCAG CCTTGTAACA AGGAGGAAGG CGGTAGGCCC
    CCCATGCCCC TGATAACCAC TGAAACGTTG CAGATGGTAC AGTTGAGGCC CGTGAAAAAG
    AACTCAGGAA CCGAGCAAGC ACTGTTATAT GAACAAGTAT CTCAGGAAAA ACTAACTCCG
    GTCGTCCCCC AGTATCATTT AAAGCCATCT GCTTTCTTGA AATCCCGAAA TAGCATAAAT
    GAAATGGAGA GTGAAAGCCA GCCTGCCTCT GTGACAAGCT CGCCGCCGCC TCCTGCCAAG
    AGCTCGAGTC AGGGTCACCG GGACGGTGCA GCCGAGCATG GTCCCCCGAG CCGCAGCCCG
    GGCGGTGCCA GGGAGGCAGA GGCTGGGCCC GGCGCCCGGC CCGCCCCGCT CCAGGAGCCC
    CCCGGCCCTT CACCCAGCAG GAAGCCACCC CCCATTTCCA AGAAGCCCAA ACTGTTTCTC
    GTGGTGCCAC CTCCGCAGAG AGATCTCACA GCGGAGCCCG CGGAGCCCGC GAGCGGAGCA
    TCACCCGGCC CCAGGCGGGG CGAGGCAGCC GAGAGTGGTA GAGCCAGCGC TGGTCCTGAT
    GAGCCGGGCT CTGGCAGCGC CGTGCTCGGG GGAGGAGCCG CAGGAGCCGC GGCCCCGGCC
    CCGGGTGGAG TGAAAGCCGA CGTCCCCATG GTGCAGCCCG ACGTGCAGCC AGCCCCCAAG
    CAGGAGGAGC CAGGTGCCGG GACCAGTGCC GACGGCGCAG GCGACGTGGA GAGCCGCCTG
    CCTCTCCAGG ACGGACGGCC TGGGCTGCCA GAGCCTGACG CAGCCGGCTC TTCCTCGGAG
    GCCTGTGACT TCCCCAGGGA AGAAGGGAGT GATGAGGTGA TGACCCCCAG TAGACCCCGG
    ACCACAGAAG ACCTTTTTGC AGCTATTCAC AGATCCAAGA GGAAAGTCCT GGGCCGGAAG
    GATTCAGATG ACGATCACTC CCGAAATCAT TCTCCCTCCC CTCCGGTGAC GCCCACCGGT
    GCGGCCCCCA GCCTGGCTTC CCCCAAGCAA GTGGGGTCAA TTCAGAGAAG TGTCCGGAAG
    AGCAGCACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AAAAGGGGAG CCGCTCGGAC
    ACCAGCGCCC GCATGTCTGC AGCTGAGATG CTAAAGAACA CAGACCCTAG ATTCCAGAGG
    TCAAGGTCAG AGCCTTCGCC AGATACTCCC GAGAGCCCGT CAAGCTGCTC CCCCAGCAAG
    AACAGAAGGG CCCAGGAGGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTCTCTGGCC CCCGGTACGG GCGCAGTCGA ACTCCACCTT CTGCAGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGAATCCA GAGCAGTCCC ATGACTGTCA TCTCAGAAGG CGAAGGGGAG
    GCCATGGAGC CTGTAGATAG CCGGGCTCAC GGGGCCCTGG GCACCGCCAG GGGGTGTTCC
    ATGAATGGAC TAGGTGGGGA GCCCACCACC CCCCTGCAGG CACAGGGTCC TGGCCCTGCT
    GATGGGATAG TCAGCGCAGA GGGCACACAT GGAACAGAAC AACGTGGAGG TCCTCAGAGG
    GAAGAGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615510.1
    CDS689..5560
    Translation

    Target ORF information:

    RefSeq Version XM_005615453.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGGGG AGAATTTCGA TCGCCAGGCC AGTCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTACAGG AGTCCCTGAC
    AACATACAGA AGGAGCTAGC ATCAGTCACT GGCCAAGATG ATGGTGGTGG TCACTCGTTG
    TACACCCCAG ACCACTGCTC CACACTGGGA AGGCTTGATA GCTATTGCTC TGCTGGGCAG
    CGCTCAGAAA CCAGGGACTC CAGCTGTCAG ACTGAGGAAG TGAAAGTTGT ACCCCCTTCA
    ATGAGAAGAA TCAGGGCACA GAAGGGGCAA GGCATTGCTG CCCAGATGAG CCACTTCTCA
    GGCTCCTCCG GGAACATGTC TGTGTTGAGT GATTCTGCAG GTATTGTGTT CCCTTCTCGC
    CTCAACAATG ATGCTGGCTT CCACAGTCTC CCACGTTCTG GAGCGAGGGT GAGTGTTCAG
    TCCCTCGAGT CACGATTGGG TGCCCTGAGC CCTGCAGAAG ACCTGGATGG CACTTATCCC
    TACCAGAGTG GTCACCCACA AGTAGATGAA AATTCAGGAC ATTTGGGAGG TGCCTCAAGG
    ACTGGAATGC TTTTGAGACC CAAGTCCCAG GAGCTGAGGC ATTTTGAGAA TGTGATCAGC
    CCAGCGTGTG TGGTCTCTCC TCATGCCACT TACTCCACCA GCATCATCCC AAATGCCACA
    CTCTCTTCCT CTTCAGAGAT TATTATTATT CACACTGCTC AGAGTTCAGG ACAGCTGGAC
    AGTAAAATCA CCAGCTCCTC TTCATACTCA AAGATAAAAT CTAGAGACCA CCTCCTCTCC
    AGGCATACTA GTAAAGATGA TCATCAGTCT CCCAGTGGCA ACTGGACAGA GGGTCACCCC
    AGCATTCTTT CACAGGCCTT AGATCCCCAC ACCTCCAGTG CAACCATGCT ATTGTCCCTC
    TGTGATTCAG TGGTTTCTCT AAATACTTCA GCAAATCGGG AGAATGGGTC CCAAGCCATG
    AGCTATAACT GTAAAAACAA CCTGAGTTCT CCGGCCCACG CCCAGGATGT GGATGGCAAG
    AGTGAGTCCA GTTATTCAGG AGGCACGGGG CCCAGCAGCT CGGAGCCCTG GGAATATAGT
    GCCCCCGGTA ACGGGCAAGC ATGCCCACTG AAGGCTCATT TGACAACGCC TGGTTACTCC
    ACTCCCGGAA GTAATGTGAG CAGCTGCAGC TTGGACCAAA CATCCACCAA AGATGATGCC
    AGATCCCTGT ATTCAGAGGA GCATGATGGC TACTACACAT CTGTGCACCC CGATCCGGGA
    CAGGGATCTG GAAACCAGTA CAATAGTGAT GGCTTTGGGA ACCCCAGGCA CAGTGTGGTC
    AATGTTTTTG ATGGAAGAGC TCAGAAAAAC CAAGGGGACC GGTCACATTA CCATGACAAG
    TCCCTTTCTC GAAACATCTC TTTGAAGAAA GCGAAGAAGC CGCCTCTGCC ACCCTCTCGG
    ACTGACTCCC TGCGCAGGAT TCCCAAGAAG GGTGTCCAGT CCAATGGGCA GGCGCTGAAC
    GAGAGCCTGA TCGCCTCGCT GCAGCACTCG CTGCAGCTGA ACCTCCCGGG CAAGGGCAGC
    AGCTCGCCCT CCCAGAGCCC CTGCAGCGAC TTTGAAGAGC CCTGGCTGCC TCGCTCCCGC
    AGCCAGAGCA CGGTGAGTGC GGGCAGCAGC ATGACCTCGG CCACCACCCC CAACGTCTAC
    TCCCTGTGCG GGGCCACGCC GTCGCAGAGT GACACGAGCA GCGTCAAGTC TGAGTACACA
    GACCCTTGGG GTTACTACAT TGACTACACA GGCATGCAGG AAGACCCCGG GCCCCCTGGC
    GGGGGCTGCT CAGCCAGCAG TGGGGCGCCG GCTGGAAATG GGCCAGTCCA CCACGTCCAG
    GAGGGATCCA GGACCCAAGT GCCTGGTGGC TCAGTCAAAC CAAAGATCAC GTCGCCAGAG
    AAGTCCCACA GAGTCACTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA
    GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAATGG GAAGGGGAAG
    ACCAAGCCCA AGGTGCCAGA AAGGAAGTCC TCTCTGATAT GTTCAGTATC CATCTCCTCA
    TCGTCCACGT CTCTGTCTTC CAATACTTCC ACGGAAGGAA GCGGGACAAT GAAGAAGCTG
    GACTCGGTGC TGGCCTCCCC CACTGCGGGT GCTTCTCTCC TTTCTCTCCC CTCACCGTGT
    GCTGCTGACA AGTCTCCTTT GCTTCCTCCT CCCCCACCTT TAGTAGATTC CCCTGACGGC
    TCTCTGCCTC AGTCTCCCCT CTTCCCCCCT CCACCACCGG AAGTTCTCAC TCCCTTCTCT
    GCCCCTGCCA GCGGGTGCCT CCCTCCTTCC CCCAGGGCAC TCAGCCCCCT TACTCTGGGT
    GCATCTGCTT CCCTGCCACT GCCCCCCGCT GCCCTGCTCT CCTCAGCTCC CCCGCCTGCC
    CCACCCCTGG ACCCTACATT GATGAAAGAT GCCAGGCCTT CTTTCAAAAA ACCTGGCCAG
    CCCGAGTGCT CCCGGGAGGG CTTCCGGCAG CCTTGTAACA AGGAGGAAGG CGGTAGGCCC
    CCCATGCCCC TGATAACCAC TGAAACGTTG CAGATGGTAC AGTTGAGGCC CGTGAAAAAG
    AACTCAGGAA CCGAGCAAGC ACTGTTATAT GAACAAGTAT CTCAGGAAAA ACTAACTCCG
    GTCGTCCCCC AGTATCATTT AAAGCCATCT GCTTTCTTGA AATCCCGAAA TAGCATAAAT
    GAAATGGAGA GTGAAAGCCA GCCTGCCTCT GTGACAAGCT CGCCGCCGCC TCCTGCCAAG
    AGCTCGAGTC AGGGTCACCG GGACGGTGCA GCCGAGCATG GTCCCCCGAG CCGCAGCCCG
    GGCGGTGCCA GGGAGGCAGA GGCTGGGCCC GGCGCCCGGC CCGCCCCGCT CCAGGAGCCC
    CCCGGCCCTT CACCCAGCAG GAAGCCACCC CCCATTTCCA AGAAGCCCAA ACTGTTTCTC
    GTGGTGCCAC CTCCGCAGAG AGATCTCACA GCGGAGCCCG CGGAGCCCGC GAGCGGAGCA
    TCACCCGGCC CCAGGCGGGG CGAGGCAGCC GAGAGTGGTA GAGCCAGCGC TGGTCCTGAT
    GAGCCGGGCT CTGGCAGCGC CGTGCTCGGG GGAGGAGCCG CAGGAGCCGC GGCCCCGGCC
    CCGGGTGGAG TGAAAGCCGA CGTCCCCATG GTGCAGCCCG ACGTGCAGCC AGCCCCCAAG
    CAGGAGGAGC CAGGTGCCGG GACCAGTGCC GACGGCGCAG GCGACGTGGA GAGCCGCCTG
    CCTCTCCAGG ACGGACGGCC TGGGCTGCCA GAGCCTGACG CAGCCGGCTC TTCCTCGGAG
    GCCTGTGACT TCCCCAGGGA AGAAGGGAGT GATGAGGTGA TGACCCCCAG TAGACCCCGG
    ACCACAGAAG ACCTTTTTGC AGCTATTCAC AGATCCAAGA GGAAAGTCCT GGGCCGGAAG
    GATTCAGATG ACGATCACTC CCGAAATCAT TCTCCCTCCC CTCCGGTGAC GCCCACCGGT
    GCGGCCCCCA GCCTGGCTTC CCCCAAGCAA GTGGGGTCAA TTCAGAGAAG TGTCCGGAAG
    AGCAGCACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AAAAGGGGAG CCGCTCGGAC
    ACCAGCGCCC GCATGTCTGC AGCTGAGATG CTAAAGAACA CAGACCCTAG ATTCCAGAGG
    TCAAGGTCAG AGCCTTCGCC AGATACTCCC GAGAGCCCGT CAAGCTGCTC CCCCAGCAAG
    AACAGAAGGG CCCAGGAGGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTCTCTGGCC CCCGGTACGG GCGCAGTCGA ACTCCACCTT CTGCAGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGAATCCA GAGCAGTCCC ATGACTGTCA TCTCAGAAGG CGAAGGGGAG
    GCCATGGAGC CTGTAGATAG CCGGGCTCAC GGGGCCCTGG GCACCGCCAG GGGGTGTTCC
    ATGAATGGAC TAGGTGGGGA GCCCACCACC CCCCTGCAGG CACAGGGTCC TGGCCCTGCT
    GATGGGATAG TCAGCGCAGA GGGCACACAT GGAACAGAAC AACGTGGAGG TCCTCAGAGG
    GAAGAGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00877
    Clone ID Related Accession (Same CDS sequence) XM_005615454.2 , XM_005615454.3
    Accession Version XM_005615454.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGCAT CAGTCACTGG CCAAGATGAT GGTGGTGGTC ACTCGTTGTA CACCCCAGAC
    CACTGCTCCA CACTGGGAAG GCTTGATAGC TATTGCTCTG CTGGGCAGCG CTCAGAAACC
    AGGGACTCCA GCTGTCAGAC TGAGGAAGTG AAAGTTGTAC CCCCTTCAAT GAGAAGAATC
    AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGAGCC ACTTCTCAGG CTCCTCCGGG
    AACATGTCTG TGTTGAGTGA TTCTGCAGGT ATTGTGTTCC CTTCTCGCCT CAACAATGAT
    GCTGGCTTCC ACAGTCTCCC ACGTTCTGGA GCGAGGGTGA GTGTTCAGTC CCTCGAGTCA
    CGATTGGGTG CCCTGAGCCC TGCAGAAGAC CTGGATGGCA CTTATCCCTA CCAGAGTGGT
    CACCCACAAG TAGATGAAAA TTCAGGACAT TTGGGAGGTG CCTCAAGGAC TGGAATGCTT
    TTGAGACCCA AGTCCCAGGA GCTGAGGCAT TTTGAGAATG TGATCAGCCC AGCGTGTGTG
    GTCTCTCCTC ATGCCACTTA CTCCACCAGC ATCATCCCAA ATGCCACACT CTCTTCCTCT
    TCAGAGATTA TTATTATTCA CACTGCTCAG AGTTCAGGAC AGCTGGACAG TAAAATCACC
    AGCTCCTCTT CATACTCAAA GATAAAATCT AGAGACCACC TCCTCTCCAG GCATACTAGT
    AAAGATGATC ATCAGTCTCC CAGTGGCAAC TGGACAGAGG GTCACCCCAG CATTCTTTCA
    CAGGCCTTAG ATCCCCACAC CTCCAGTGCA ACCATGCTAT TGTCCCTCTG TGATTCAGTG
    GTTTCTCTAA ATACTTCAGC AAATCGGGAG AATGGGTCCC AAGCCATGAG CTATAACTGT
    AAAAACAACC TGAGTTCTCC GGCCCACGCC CAGGATGTGG ATGGCAAGAG TGAGTCCAGT
    TATTCAGGAG GCACGGGGCC CAGCAGCTCG GAGCCCTGGG AATATAGTGC CCCCGGTAAC
    GGGCAAGCAT GCCCACTGAA GGCTCATTTG ACAACGCCTG GTTACTCCAC TCCCGGAAGT
    AATGTGAGCA GCTGCAGCTT GGACCAAACA TCCACCAAAG ATGATGCCAG ATCCCTGTAT
    TCAGAGGAGC ATGATGGCTA CTACACATCT GTGCACCCCG ATCCGGGACA GGGATCTGGA
    AACCAGTACA ATAGTGATGG CTTTGGGAAC CCCAGGCACA GTGTGGTCAA TGTTTTTGAT
    GGAAGAGCTC AGAAAAACCA AGGGGACCGG TCACATTACC ATGACAAGTC CCTTTCTCGA
    AACATCTCTT TGAAGAAAGC GAAGAAGCCG CCTCTGCCAC CCTCTCGGAC TGACTCCCTG
    CGCAGGATTC CCAAGAAGGG TGTCCAGTCC AATGGGCAGG CGCTGAACGA GAGCCTGATC
    GCCTCGCTGC AGCACTCGCT GCAGCTGAAC CTCCCGGGCA AGGGCAGCAG CTCGCCCTCC
    CAGAGCCCCT GCAGCGACTT TGAAGAGCCC TGGCTGCCTC GCTCCCGCAG CCAGAGCACG
    GTGAGTGCGG GCAGCAGCAT GACCTCGGCC ACCACCCCCA ACGTCTACTC CCTGTGCGGG
    GCCACGCCGT CGCAGAGTGA CACGAGCAGC GTCAAGTCTG AGTACACAGA CCCTTGGGGT
    TACTACATTG ACTACACAGG CATGCAGGAA GACCCCGGGC CCCCTGGCGG GGGCTGCTCA
    GCCAGCAGTG GGGCGCCGGC TGGAAATGGG CCAGTCCACC ACGTCCAGGA GGGATCCAGG
    ACCCAAGTGC CTGGTGGCTC AGTCAAACCA AAGATCACGT CGCCAGAGAA GTCCCACAGA
    GTCACTTCTC CATCCAGTGG GTATTCCAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AGTGTCACCA GCAAATGGGA AGGGGAAGAC CAAGCCCAAG
    GTGCCAGAAA GGAAGTCCTC TCTGATATGT TCAGTATCCA TCTCCTCATC GTCCACGTCT
    CTGTCTTCCA ATACTTCCAC GGAAGGAAGC GGGACAATGA AGAAGCTGGA CTCGGTGCTG
    GCCTCCCCCA CTGCGGGTGC TTCTCTCCTT TCTCTCCCCT CACCGTGTGC TGCTGACAAG
    TCTCCTTTGC TTCCTCCTCC CCCACCTTTA GTAGATTCCC CTGACGGCTC TCTGCCTCAG
    TCTCCCCTCT TCCCCCCTCC ACCACCGGAA GTTCTCACTC CCTTCTCTGC CCCTGCCAGC
    GGGTGCCTCC CTCCTTCCCC CAGGGCACTC AGCCCCCTTA CTCTGGGTGC ATCTGCTTCC
    CTGCCACTGC CCCCCGCTGC CCTGCTCTCC TCAGCTCCCC CGCCTGCCCC ACCCCTGGAC
    CCTACATTGA TGAAAGATGC CAGGCCTTCT TTCAAAAAAC CTGGCCAGCC CGAGTGCTCC
    CGGGAGGGCT TCCGGCAGCC TTGTAACAAG GAGGAAGGCG GTAGGCCCCC CATGCCCCTG
    ATAACCACTG AAACGTTGCA GATGGTACAG TTGAGGCCCG TGAAAAAGAA CTCAGGAACC
    GAGCAAGCAC TGTTATATGA ACAAGTATCT CAGGAAAAAC TAACTCCGGT CGTCCCCCAG
    TATCATTTAA AGCCATCTGC TTTCTTGAAA TCCCGAAATA GCATAAATGA AATGGAGAGT
    GAAAGCCAGC CTGCCTCTGT GACAAGCTCG CCGCCGCCTC CTGCCAAGAG CTCGAGTCAG
    GGTCACCGGG ACGGTGCAGC CGAGCATGGT CCCCCGAGCC GCAGCCCGGG CGGTGCCAGG
    GAGGCAGAGG CTGGGCCCGG CGCCCGGCCC GCCCCGCTCC AGGAGCCCCC CGGCCCTTCA
    CCCAGCAGGA AGCCACCCCC CATTTCCAAG AAGCCCAAAC TGTTTCTCGT GGTGCCACCT
    CCGCAGAGAG ATCTCACAGC GGAGCCCGCG GAGCCCGCGA GCGGAGCATC ACCCGGCCCC
    AGGCGGGGCG AGGCAGCCGA GAGTGGTAGA GCCAGCGCTG GTCCTGATGA GCCGGGCTCT
    GGCAGCGCCG TGCTCGGGGG AGGAGCCGCA GGAGCCGCGG CCCCGGCCCC GGGTGGAGTG
    AAAGCCGACG TCCCCATGGT GCAGCCCGAC GTGCAGCCAG CCCCCAAGCA GGAGGAGCCA
    GGTGCCGGGA CCAGTGCCGA CGGCGCAGGC GACGTGGAGA GCCGCCTGCC TCTCCAGGAC
    GGACGGCCTG GGCTGCCAGA GCCTGACGCA GCCGGCTCTT CCTCGGAGGC CTGTGACTTC
    CCCAGGGAAG AAGGGAGTGA TGAGGTGATG ACCCCCAGTA GACCCCGGAC CACAGAAGAC
    CTTTTTGCAG CTATTCACAG ATCCAAGAGG AAAGTCCTGG GCCGGAAGGA TTCAGATGAC
    GATCACTCCC GAAATCATTC TCCCTCCCCT CCGGTGACGC CCACCGGTGC GGCCCCCAGC
    CTGGCTTCCC CCAAGCAAGT GGGGTCAATT CAGAGAAGTG TCCGGAAGAG CAGCACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAA AAGGGGAGCC GCTCGGACAC CAGCGCCCGC
    ATGTCTGCAG CTGAGATGCT AAAGAACACA GACCCTAGAT TCCAGAGGTC AAGGTCAGAG
    CCTTCGCCAG ATACTCCCGA GAGCCCGTCA AGCTGCTCCC CCAGCAAGAA CAGAAGGGCC
    CAGGAGGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT CTCTGGCCCC
    CGGTACGGGC GCAGTCGAAC TCCACCTTCT GCAGCCAGCA GCAGGTACAG CATGCGGAAC
    CGAATCCAGA GCAGTCCCAT GACTGTCATC TCAGAAGGCG AAGGGGAGGC CATGGAGCCT
    GTAGATAGCC GGGCTCACGG GGCCCTGGGC ACCGCCAGGG GGTGTTCCAT GAATGGACTA
    GGTGGGGAGC CCACCACCCC CCTGCAGGCA CAGGGTCCTG GCCCTGCTGA TGGGATAGTC
    AGCGCAGAGG GCACACATGG AACAGAACAA CGTGGAGGTC CTCAGAGGGA AGAGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615511.1
    CDS688..5427
    Translation

    Target ORF information:

    RefSeq Version XM_005615454.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615454.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGCAT CAGTCACTGG CCAAGATGAT GGTGGTGGTC ACTCGTTGTA CACCCCAGAC
    CACTGCTCCA CACTGGGAAG GCTTGATAGC TATTGCTCTG CTGGGCAGCG CTCAGAAACC
    AGGGACTCCA GCTGTCAGAC TGAGGAAGTG AAAGTTGTAC CCCCTTCAAT GAGAAGAATC
    AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGAGCC ACTTCTCAGG CTCCTCCGGG
    AACATGTCTG TGTTGAGTGA TTCTGCAGGT ATTGTGTTCC CTTCTCGCCT CAACAATGAT
    GCTGGCTTCC ACAGTCTCCC ACGTTCTGGA GCGAGGGTGA GTGTTCAGTC CCTCGAGTCA
    CGATTGGGTG CCCTGAGCCC TGCAGAAGAC CTGGATGGCA CTTATCCCTA CCAGAGTGGT
    CACCCACAAG TAGATGAAAA TTCAGGACAT TTGGGAGGTG CCTCAAGGAC TGGAATGCTT
    TTGAGACCCA AGTCCCAGGA GCTGAGGCAT TTTGAGAATG TGATCAGCCC AGCGTGTGTG
    GTCTCTCCTC ATGCCACTTA CTCCACCAGC ATCATCCCAA ATGCCACACT CTCTTCCTCT
    TCAGAGATTA TTATTATTCA CACTGCTCAG AGTTCAGGAC AGCTGGACAG TAAAATCACC
    AGCTCCTCTT CATACTCAAA GATAAAATCT AGAGACCACC TCCTCTCCAG GCATACTAGT
    AAAGATGATC ATCAGTCTCC CAGTGGCAAC TGGACAGAGG GTCACCCCAG CATTCTTTCA
    CAGGCCTTAG ATCCCCACAC CTCCAGTGCA ACCATGCTAT TGTCCCTCTG TGATTCAGTG
    GTTTCTCTAA ATACTTCAGC AAATCGGGAG AATGGGTCCC AAGCCATGAG CTATAACTGT
    AAAAACAACC TGAGTTCTCC GGCCCACGCC CAGGATGTGG ATGGCAAGAG TGAGTCCAGT
    TATTCAGGAG GCACGGGGCC CAGCAGCTCG GAGCCCTGGG AATATAGTGC CCCCGGTAAC
    GGGCAAGCAT GCCCACTGAA GGCTCATTTG ACAACGCCTG GTTACTCCAC TCCCGGAAGT
    AATGTGAGCA GCTGCAGCTT GGACCAAACA TCCACCAAAG ATGATGCCAG ATCCCTGTAT
    TCAGAGGAGC ATGATGGCTA CTACACATCT GTGCACCCCG ATCCGGGACA GGGATCTGGA
    AACCAGTACA ATAGTGATGG CTTTGGGAAC CCCAGGCACA GTGTGGTCAA TGTTTTTGAT
    GGAAGAGCTC AGAAAAACCA AGGGGACCGG TCACATTACC ATGACAAGTC CCTTTCTCGA
    AACATCTCTT TGAAGAAAGC GAAGAAGCCG CCTCTGCCAC CCTCTCGGAC TGACTCCCTG
    CGCAGGATTC CCAAGAAGGG TGTCCAGTCC AATGGGCAGG CGCTGAACGA GAGCCTGATC
    GCCTCGCTGC AGCACTCGCT GCAGCTGAAC CTCCCGGGCA AGGGCAGCAG CTCGCCCTCC
    CAGAGCCCCT GCAGCGACTT TGAAGAGCCC TGGCTGCCTC GCTCCCGCAG CCAGAGCACG
    GTGAGTGCGG GCAGCAGCAT GACCTCGGCC ACCACCCCCA ACGTCTACTC CCTGTGCGGG
    GCCACGCCGT CGCAGAGTGA CACGAGCAGC GTCAAGTCTG AGTACACAGA CCCTTGGGGT
    TACTACATTG ACTACACAGG CATGCAGGAA GACCCCGGGC CCCCTGGCGG GGGCTGCTCA
    GCCAGCAGTG GGGCGCCGGC TGGAAATGGG CCAGTCCACC ACGTCCAGGA GGGATCCAGG
    ACCCAAGTGC CTGGTGGCTC AGTCAAACCA AAGATCACGT CGCCAGAGAA GTCCCACAGA
    GTCACTTCTC CATCCAGTGG GTATTCCAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AGTGTCACCA GCAAATGGGA AGGGGAAGAC CAAGCCCAAG
    GTGCCAGAAA GGAAGTCCTC TCTGATATGT TCAGTATCCA TCTCCTCATC GTCCACGTCT
    CTGTCTTCCA ATACTTCCAC GGAAGGAAGC GGGACAATGA AGAAGCTGGA CTCGGTGCTG
    GCCTCCCCCA CTGCGGGTGC TTCTCTCCTT TCTCTCCCCT CACCGTGTGC TGCTGACAAG
    TCTCCTTTGC TTCCTCCTCC CCCACCTTTA GTAGATTCCC CTGACGGCTC TCTGCCTCAG
    TCTCCCCTCT TCCCCCCTCC ACCACCGGAA GTTCTCACTC CCTTCTCTGC CCCTGCCAGC
    GGGTGCCTCC CTCCTTCCCC CAGGGCACTC AGCCCCCTTA CTCTGGGTGC ATCTGCTTCC
    CTGCCACTGC CCCCCGCTGC CCTGCTCTCC TCAGCTCCCC CGCCTGCCCC ACCCCTGGAC
    CCTACATTGA TGAAAGATGC CAGGCCTTCT TTCAAAAAAC CTGGCCAGCC CGAGTGCTCC
    CGGGAGGGCT TCCGGCAGCC TTGTAACAAG GAGGAAGGCG GTAGGCCCCC CATGCCCCTG
    ATAACCACTG AAACGTTGCA GATGGTACAG TTGAGGCCCG TGAAAAAGAA CTCAGGAACC
    GAGCAAGCAC TGTTATATGA ACAAGTATCT CAGGAAAAAC TAACTCCGGT CGTCCCCCAG
    TATCATTTAA AGCCATCTGC TTTCTTGAAA TCCCGAAATA GCATAAATGA AATGGAGAGT
    GAAAGCCAGC CTGCCTCTGT GACAAGCTCG CCGCCGCCTC CTGCCAAGAG CTCGAGTCAG
    GGTCACCGGG ACGGTGCAGC CGAGCATGGT CCCCCGAGCC GCAGCCCGGG CGGTGCCAGG
    GAGGCAGAGG CTGGGCCCGG CGCCCGGCCC GCCCCGCTCC AGGAGCCCCC CGGCCCTTCA
    CCCAGCAGGA AGCCACCCCC CATTTCCAAG AAGCCCAAAC TGTTTCTCGT GGTGCCACCT
    CCGCAGAGAG ATCTCACAGC GGAGCCCGCG GAGCCCGCGA GCGGAGCATC ACCCGGCCCC
    AGGCGGGGCG AGGCAGCCGA GAGTGGTAGA GCCAGCGCTG GTCCTGATGA GCCGGGCTCT
    GGCAGCGCCG TGCTCGGGGG AGGAGCCGCA GGAGCCGCGG CCCCGGCCCC GGGTGGAGTG
    AAAGCCGACG TCCCCATGGT GCAGCCCGAC GTGCAGCCAG CCCCCAAGCA GGAGGAGCCA
    GGTGCCGGGA CCAGTGCCGA CGGCGCAGGC GACGTGGAGA GCCGCCTGCC TCTCCAGGAC
    GGACGGCCTG GGCTGCCAGA GCCTGACGCA GCCGGCTCTT CCTCGGAGGC CTGTGACTTC
    CCCAGGGAAG AAGGGAGTGA TGAGGTGATG ACCCCCAGTA GACCCCGGAC CACAGAAGAC
    CTTTTTGCAG CTATTCACAG ATCCAAGAGG AAAGTCCTGG GCCGGAAGGA TTCAGATGAC
    GATCACTCCC GAAATCATTC TCCCTCCCCT CCGGTGACGC CCACCGGTGC GGCCCCCAGC
    CTGGCTTCCC CCAAGCAAGT GGGGTCAATT CAGAGAAGTG TCCGGAAGAG CAGCACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAA AAGGGGAGCC GCTCGGACAC CAGCGCCCGC
    ATGTCTGCAG CTGAGATGCT AAAGAACACA GACCCTAGAT TCCAGAGGTC AAGGTCAGAG
    CCTTCGCCAG ATACTCCCGA GAGCCCGTCA AGCTGCTCCC CCAGCAAGAA CAGAAGGGCC
    CAGGAGGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT CTCTGGCCCC
    CGGTACGGGC GCAGTCGAAC TCCACCTTCT GCAGCCAGCA GCAGGTACAG CATGCGGAAC
    CGAATCCAGA GCAGTCCCAT GACTGTCATC TCAGAAGGCG AAGGGGAGGC CATGGAGCCT
    GTAGATAGCC GGGCTCACGG GGCCCTGGGC ACCGCCAGGG GGTGTTCCAT GAATGGACTA
    GGTGGGGAGC CCACCACCCC CCTGCAGGCA CAGGGTCCTG GCCCTGCTGA TGGGATAGTC
    AGCGCAGAGG GCACACATGG AACAGAACAA CGTGGAGGTC CTCAGAGGGA AGAGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00877
    Clone ID Related Accession (Same CDS sequence) XM_005615454.2 , XM_005615454.3
    Accession Version XM_005615454.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGCAT CAGTCACTGG CCAAGATGAT GGTGGTGGTC ACTCGTTGTA CACCCCAGAC
    CACTGCTCCA CACTGGGAAG GCTTGATAGC TATTGCTCTG CTGGGCAGCG CTCAGAAACC
    AGGGACTCCA GCTGTCAGAC TGAGGAAGTG AAAGTTGTAC CCCCTTCAAT GAGAAGAATC
    AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGAGCC ACTTCTCAGG CTCCTCCGGG
    AACATGTCTG TGTTGAGTGA TTCTGCAGGT ATTGTGTTCC CTTCTCGCCT CAACAATGAT
    GCTGGCTTCC ACAGTCTCCC ACGTTCTGGA GCGAGGGTGA GTGTTCAGTC CCTCGAGTCA
    CGATTGGGTG CCCTGAGCCC TGCAGAAGAC CTGGATGGCA CTTATCCCTA CCAGAGTGGT
    CACCCACAAG TAGATGAAAA TTCAGGACAT TTGGGAGGTG CCTCAAGGAC TGGAATGCTT
    TTGAGACCCA AGTCCCAGGA GCTGAGGCAT TTTGAGAATG TGATCAGCCC AGCGTGTGTG
    GTCTCTCCTC ATGCCACTTA CTCCACCAGC ATCATCCCAA ATGCCACACT CTCTTCCTCT
    TCAGAGATTA TTATTATTCA CACTGCTCAG AGTTCAGGAC AGCTGGACAG TAAAATCACC
    AGCTCCTCTT CATACTCAAA GATAAAATCT AGAGACCACC TCCTCTCCAG GCATACTAGT
    AAAGATGATC ATCAGTCTCC CAGTGGCAAC TGGACAGAGG GTCACCCCAG CATTCTTTCA
    CAGGCCTTAG ATCCCCACAC CTCCAGTGCA ACCATGCTAT TGTCCCTCTG TGATTCAGTG
    GTTTCTCTAA ATACTTCAGC AAATCGGGAG AATGGGTCCC AAGCCATGAG CTATAACTGT
    AAAAACAACC TGAGTTCTCC GGCCCACGCC CAGGATGTGG ATGGCAAGAG TGAGTCCAGT
    TATTCAGGAG GCACGGGGCC CAGCAGCTCG GAGCCCTGGG AATATAGTGC CCCCGGTAAC
    GGGCAAGCAT GCCCACTGAA GGCTCATTTG ACAACGCCTG GTTACTCCAC TCCCGGAAGT
    AATGTGAGCA GCTGCAGCTT GGACCAAACA TCCACCAAAG ATGATGCCAG ATCCCTGTAT
    TCAGAGGAGC ATGATGGCTA CTACACATCT GTGCACCCCG ATCCGGGACA GGGATCTGGA
    AACCAGTACA ATAGTGATGG CTTTGGGAAC CCCAGGCACA GTGTGGTCAA TGTTTTTGAT
    GGAAGAGCTC AGAAAAACCA AGGGGACCGG TCACATTACC ATGACAAGTC CCTTTCTCGA
    AACATCTCTT TGAAGAAAGC GAAGAAGCCG CCTCTGCCAC CCTCTCGGAC TGACTCCCTG
    CGCAGGATTC CCAAGAAGGG TGTCCAGTCC AATGGGCAGG CGCTGAACGA GAGCCTGATC
    GCCTCGCTGC AGCACTCGCT GCAGCTGAAC CTCCCGGGCA AGGGCAGCAG CTCGCCCTCC
    CAGAGCCCCT GCAGCGACTT TGAAGAGCCC TGGCTGCCTC GCTCCCGCAG CCAGAGCACG
    GTGAGTGCGG GCAGCAGCAT GACCTCGGCC ACCACCCCCA ACGTCTACTC CCTGTGCGGG
    GCCACGCCGT CGCAGAGTGA CACGAGCAGC GTCAAGTCTG AGTACACAGA CCCTTGGGGT
    TACTACATTG ACTACACAGG CATGCAGGAA GACCCCGGGC CCCCTGGCGG GGGCTGCTCA
    GCCAGCAGTG GGGCGCCGGC TGGAAATGGG CCAGTCCACC ACGTCCAGGA GGGATCCAGG
    ACCCAAGTGC CTGGTGGCTC AGTCAAACCA AAGATCACGT CGCCAGAGAA GTCCCACAGA
    GTCACTTCTC CATCCAGTGG GTATTCCAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AGTGTCACCA GCAAATGGGA AGGGGAAGAC CAAGCCCAAG
    GTGCCAGAAA GGAAGTCCTC TCTGATATGT TCAGTATCCA TCTCCTCATC GTCCACGTCT
    CTGTCTTCCA ATACTTCCAC GGAAGGAAGC GGGACAATGA AGAAGCTGGA CTCGGTGCTG
    GCCTCCCCCA CTGCGGGTGC TTCTCTCCTT TCTCTCCCCT CACCGTGTGC TGCTGACAAG
    TCTCCTTTGC TTCCTCCTCC CCCACCTTTA GTAGATTCCC CTGACGGCTC TCTGCCTCAG
    TCTCCCCTCT TCCCCCCTCC ACCACCGGAA GTTCTCACTC CCTTCTCTGC CCCTGCCAGC
    GGGTGCCTCC CTCCTTCCCC CAGGGCACTC AGCCCCCTTA CTCTGGGTGC ATCTGCTTCC
    CTGCCACTGC CCCCCGCTGC CCTGCTCTCC TCAGCTCCCC CGCCTGCCCC ACCCCTGGAC
    CCTACATTGA TGAAAGATGC CAGGCCTTCT TTCAAAAAAC CTGGCCAGCC CGAGTGCTCC
    CGGGAGGGCT TCCGGCAGCC TTGTAACAAG GAGGAAGGCG GTAGGCCCCC CATGCCCCTG
    ATAACCACTG AAACGTTGCA GATGGTACAG TTGAGGCCCG TGAAAAAGAA CTCAGGAACC
    GAGCAAGCAC TGTTATATGA ACAAGTATCT CAGGAAAAAC TAACTCCGGT CGTCCCCCAG
    TATCATTTAA AGCCATCTGC TTTCTTGAAA TCCCGAAATA GCATAAATGA AATGGAGAGT
    GAAAGCCAGC CTGCCTCTGT GACAAGCTCG CCGCCGCCTC CTGCCAAGAG CTCGAGTCAG
    GGTCACCGGG ACGGTGCAGC CGAGCATGGT CCCCCGAGCC GCAGCCCGGG CGGTGCCAGG
    GAGGCAGAGG CTGGGCCCGG CGCCCGGCCC GCCCCGCTCC AGGAGCCCCC CGGCCCTTCA
    CCCAGCAGGA AGCCACCCCC CATTTCCAAG AAGCCCAAAC TGTTTCTCGT GGTGCCACCT
    CCGCAGAGAG ATCTCACAGC GGAGCCCGCG GAGCCCGCGA GCGGAGCATC ACCCGGCCCC
    AGGCGGGGCG AGGCAGCCGA GAGTGGTAGA GCCAGCGCTG GTCCTGATGA GCCGGGCTCT
    GGCAGCGCCG TGCTCGGGGG AGGAGCCGCA GGAGCCGCGG CCCCGGCCCC GGGTGGAGTG
    AAAGCCGACG TCCCCATGGT GCAGCCCGAC GTGCAGCCAG CCCCCAAGCA GGAGGAGCCA
    GGTGCCGGGA CCAGTGCCGA CGGCGCAGGC GACGTGGAGA GCCGCCTGCC TCTCCAGGAC
    GGACGGCCTG GGCTGCCAGA GCCTGACGCA GCCGGCTCTT CCTCGGAGGC CTGTGACTTC
    CCCAGGGAAG AAGGGAGTGA TGAGGTGATG ACCCCCAGTA GACCCCGGAC CACAGAAGAC
    CTTTTTGCAG CTATTCACAG ATCCAAGAGG AAAGTCCTGG GCCGGAAGGA TTCAGATGAC
    GATCACTCCC GAAATCATTC TCCCTCCCCT CCGGTGACGC CCACCGGTGC GGCCCCCAGC
    CTGGCTTCCC CCAAGCAAGT GGGGTCAATT CAGAGAAGTG TCCGGAAGAG CAGCACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAA AAGGGGAGCC GCTCGGACAC CAGCGCCCGC
    ATGTCTGCAG CTGAGATGCT AAAGAACACA GACCCTAGAT TCCAGAGGTC AAGGTCAGAG
    CCTTCGCCAG ATACTCCCGA GAGCCCGTCA AGCTGCTCCC CCAGCAAGAA CAGAAGGGCC
    CAGGAGGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT CTCTGGCCCC
    CGGTACGGGC GCAGTCGAAC TCCACCTTCT GCAGCCAGCA GCAGGTACAG CATGCGGAAC
    CGAATCCAGA GCAGTCCCAT GACTGTCATC TCAGAAGGCG AAGGGGAGGC CATGGAGCCT
    GTAGATAGCC GGGCTCACGG GGCCCTGGGC ACCGCCAGGG GGTGTTCCAT GAATGGACTA
    GGTGGGGAGC CCACCACCCC CCTGCAGGCA CAGGGTCCTG GCCCTGCTGA TGGGATAGTC
    AGCGCAGAGG GCACACATGG AACAGAACAA CGTGGAGGTC CTCAGAGGGA AGAGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615511.1
    CDS682..5421
    Translation

    Target ORF information:

    RefSeq Version XM_005615454.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615454.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAAAGG AAGGCTCGTT CAGACTCAAG TCAAATTCAG GGTCTCTCTC ACGTGCTGTG 
    AGTTGGATAA ATTTCTCCTC CTTGTCCAGG CAGACAAAGA GGCTGTTTCG CAGTGATGGA
    GAGCTCTCCG TCTGTGGGCA GCAGGTGGAA GCAGATGATG AAAACTGGAA TTACAGAACC
    CAGCCCAGAA AAGCGGTTTC CAACCTAGAT GAGGAGAGTA GATGGACAGT CCACTACACT
    GCTCCATGGC ACCAGCAGGA GAATGTGTTC CTTCCCACCA CAAGACCACC CTGTGTAGAG
    GACCTGCACC GCCAAGCCAA GCTCAACCTC AAATCAGTAT TGAGGGAATG CGATAAACTA
    AGACGAGATG GCTACCGAAG TTCTCAGTAC TACTCCCAGG GGCCCACCTT TGCAGCCAAT
    GCCAGCCCAC TCTGTGATGA TTATCAAGAT GAGGATGAAG AAACAGATCA AAAGTGTTCT
    GTTTCTTCAT CAGAAGAAGA AAGACTTATT TCCATCAGGA GACCTAAAAC GCCAACCTCA
    AGTGACTTCT CTGACCTTAA TACTCAAACA AACTGGACCA AGTCACTTCC TTTGCCAACA
    CCAGAAGAGA AGATGCGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TGCCATTAAC
    ATAACTGCAT CAGTCACTGG CCAAGATGAT GGTGGTGGTC ACTCGTTGTA CACCCCAGAC
    CACTGCTCCA CACTGGGAAG GCTTGATAGC TATTGCTCTG CTGGGCAGCG CTCAGAAACC
    AGGGACTCCA GCTGTCAGAC TGAGGAAGTG AAAGTTGTAC CCCCTTCAAT GAGAAGAATC
    AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGAGCC ACTTCTCAGG CTCCTCCGGG
    AACATGTCTG TGTTGAGTGA TTCTGCAGGT ATTGTGTTCC CTTCTCGCCT CAACAATGAT
    GCTGGCTTCC ACAGTCTCCC ACGTTCTGGA GCGAGGGTGA GTGTTCAGTC CCTCGAGTCA
    CGATTGGGTG CCCTGAGCCC TGCAGAAGAC CTGGATGGCA CTTATCCCTA CCAGAGTGGT
    CACCCACAAG TAGATGAAAA TTCAGGACAT TTGGGAGGTG CCTCAAGGAC TGGAATGCTT
    TTGAGACCCA AGTCCCAGGA GCTGAGGCAT TTTGAGAATG TGATCAGCCC AGCGTGTGTG
    GTCTCTCCTC ATGCCACTTA CTCCACCAGC ATCATCCCAA ATGCCACACT CTCTTCCTCT
    TCAGAGATTA TTATTATTCA CACTGCTCAG AGTTCAGGAC AGCTGGACAG TAAAATCACC
    AGCTCCTCTT CATACTCAAA GATAAAATCT AGAGACCACC TCCTCTCCAG GCATACTAGT
    AAAGATGATC ATCAGTCTCC CAGTGGCAAC TGGACAGAGG GTCACCCCAG CATTCTTTCA
    CAGGCCTTAG ATCCCCACAC CTCCAGTGCA ACCATGCTAT TGTCCCTCTG TGATTCAGTG
    GTTTCTCTAA ATACTTCAGC AAATCGGGAG AATGGGTCCC AAGCCATGAG CTATAACTGT
    AAAAACAACC TGAGTTCTCC GGCCCACGCC CAGGATGTGG ATGGCAAGAG TGAGTCCAGT
    TATTCAGGAG GCACGGGGCC CAGCAGCTCG GAGCCCTGGG AATATAGTGC CCCCGGTAAC
    GGGCAAGCAT GCCCACTGAA GGCTCATTTG ACAACGCCTG GTTACTCCAC TCCCGGAAGT
    AATGTGAGCA GCTGCAGCTT GGACCAAACA TCCACCAAAG ATGATGCCAG ATCCCTGTAT
    TCAGAGGAGC ATGATGGCTA CTACACATCT GTGCACCCCG ATCCGGGACA GGGATCTGGA
    AACCAGTACA ATAGTGATGG CTTTGGGAAC CCCAGGCACA GTGTGGTCAA TGTTTTTGAT
    GGAAGAGCTC AGAAAAACCA AGGGGACCGG TCACATTACC ATGACAAGTC CCTTTCTCGA
    AACATCTCTT TGAAGAAAGC GAAGAAGCCG CCTCTGCCAC CCTCTCGGAC TGACTCCCTG
    CGCAGGATTC CCAAGAAGGG TGTCCAGTCC AATGGGCAGG CGCTGAACGA GAGCCTGATC
    GCCTCGCTGC AGCACTCGCT GCAGCTGAAC CTCCCGGGCA AGGGCAGCAG CTCGCCCTCC
    CAGAGCCCCT GCAGCGACTT TGAAGAGCCC TGGCTGCCTC GCTCCCGCAG CCAGAGCACG
    GTGAGTGCGG GCAGCAGCAT GACCTCGGCC ACCACCCCCA ACGTCTACTC CCTGTGCGGG
    GCCACGCCGT CGCAGAGTGA CACGAGCAGC GTCAAGTCTG AGTACACAGA CCCTTGGGGT
    TACTACATTG ACTACACAGG CATGCAGGAA GACCCCGGGC CCCCTGGCGG GGGCTGCTCA
    GCCAGCAGTG GGGCGCCGGC TGGAAATGGG CCAGTCCACC ACGTCCAGGA GGGATCCAGG
    ACCCAAGTGC CTGGTGGCTC AGTCAAACCA AAGATCACGT CGCCAGAGAA GTCCCACAGA
    GTCACTTCTC CATCCAGTGG GTATTCCAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AGTGTCACCA GCAAATGGGA AGGGGAAGAC CAAGCCCAAG
    GTGCCAGAAA GGAAGTCCTC TCTGATATGT TCAGTATCCA TCTCCTCATC GTCCACGTCT
    CTGTCTTCCA ATACTTCCAC GGAAGGAAGC GGGACAATGA AGAAGCTGGA CTCGGTGCTG
    GCCTCCCCCA CTGCGGGTGC TTCTCTCCTT TCTCTCCCCT CACCGTGTGC TGCTGACAAG
    TCTCCTTTGC TTCCTCCTCC CCCACCTTTA GTAGATTCCC CTGACGGCTC TCTGCCTCAG
    TCTCCCCTCT TCCCCCCTCC ACCACCGGAA GTTCTCACTC CCTTCTCTGC CCCTGCCAGC
    GGGTGCCTCC CTCCTTCCCC CAGGGCACTC AGCCCCCTTA CTCTGGGTGC ATCTGCTTCC
    CTGCCACTGC CCCCCGCTGC CCTGCTCTCC TCAGCTCCCC CGCCTGCCCC ACCCCTGGAC
    CCTACATTGA TGAAAGATGC CAGGCCTTCT TTCAAAAAAC CTGGCCAGCC CGAGTGCTCC
    CGGGAGGGCT TCCGGCAGCC TTGTAACAAG GAGGAAGGCG GTAGGCCCCC CATGCCCCTG
    ATAACCACTG AAACGTTGCA GATGGTACAG TTGAGGCCCG TGAAAAAGAA CTCAGGAACC
    GAGCAAGCAC TGTTATATGA ACAAGTATCT CAGGAAAAAC TAACTCCGGT CGTCCCCCAG
    TATCATTTAA AGCCATCTGC TTTCTTGAAA TCCCGAAATA GCATAAATGA AATGGAGAGT
    GAAAGCCAGC CTGCCTCTGT GACAAGCTCG CCGCCGCCTC CTGCCAAGAG CTCGAGTCAG
    GGTCACCGGG ACGGTGCAGC CGAGCATGGT CCCCCGAGCC GCAGCCCGGG CGGTGCCAGG
    GAGGCAGAGG CTGGGCCCGG CGCCCGGCCC GCCCCGCTCC AGGAGCCCCC CGGCCCTTCA
    CCCAGCAGGA AGCCACCCCC CATTTCCAAG AAGCCCAAAC TGTTTCTCGT GGTGCCACCT
    CCGCAGAGAG ATCTCACAGC GGAGCCCGCG GAGCCCGCGA GCGGAGCATC ACCCGGCCCC
    AGGCGGGGCG AGGCAGCCGA GAGTGGTAGA GCCAGCGCTG GTCCTGATGA GCCGGGCTCT
    GGCAGCGCCG TGCTCGGGGG AGGAGCCGCA GGAGCCGCGG CCCCGGCCCC GGGTGGAGTG
    AAAGCCGACG TCCCCATGGT GCAGCCCGAC GTGCAGCCAG CCCCCAAGCA GGAGGAGCCA
    GGTGCCGGGA CCAGTGCCGA CGGCGCAGGC GACGTGGAGA GCCGCCTGCC TCTCCAGGAC
    GGACGGCCTG GGCTGCCAGA GCCTGACGCA GCCGGCTCTT CCTCGGAGGC CTGTGACTTC
    CCCAGGGAAG AAGGGAGTGA TGAGGTGATG ACCCCCAGTA GACCCCGGAC CACAGAAGAC
    CTTTTTGCAG CTATTCACAG ATCCAAGAGG AAAGTCCTGG GCCGGAAGGA TTCAGATGAC
    GATCACTCCC GAAATCATTC TCCCTCCCCT CCGGTGACGC CCACCGGTGC GGCCCCCAGC
    CTGGCTTCCC CCAAGCAAGT GGGGTCAATT CAGAGAAGTG TCCGGAAGAG CAGCACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAA AAGGGGAGCC GCTCGGACAC CAGCGCCCGC
    ATGTCTGCAG CTGAGATGCT AAAGAACACA GACCCTAGAT TCCAGAGGTC AAGGTCAGAG
    CCTTCGCCAG ATACTCCCGA GAGCCCGTCA AGCTGCTCCC CCAGCAAGAA CAGAAGGGCC
    CAGGAGGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT CTCTGGCCCC
    CGGTACGGGC GCAGTCGAAC TCCACCTTCT GCAGCCAGCA GCAGGTACAG CATGCGGAAC
    CGAATCCAGA GCAGTCCCAT GACTGTCATC TCAGAAGGCG AAGGGGAGGC CATGGAGCCT
    GTAGATAGCC GGGCTCACGG GGCCCTGGGC ACCGCCAGGG GGTGTTCCAT GAATGGACTA
    GGTGGGGAGC CCACCACCCC CCTGCAGGCA CAGGGTCCTG GCCCTGCTGA TGGGATAGTC
    AGCGCAGAGG GCACACATGG AACAGAACAA CGTGGAGGTC CTCAGAGGGA AGAGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00878
    Clone ID Related Accession (Same CDS sequence) XM_005615455.2 , XM_005615455.3
    Accession Version XM_005615455.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCTGCC TGAAAGCGGT TTCCAACCTA GATGAGGAGA GTAGATGGAC AGTCCACTAC 
    ACTGCTCCAT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC ACCCTGTGTA
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TATTGAGGGA ATGCGATAAA
    CTAAGACGAG ATGGCTACCG AAGTTCTCAG TACTACTCCC AGGGGCCCAC CTTTGCAGCC
    AATGCCAGCC CACTCTGTGA TGATTATCAA GATGAGGATG AAGAAACAGA TCAAAAGTGT
    TCTGTTTCTT CATCAGAAGA AGAAAGACTT ATTTCCATCA GGAGACCTAA AACGCCAACC
    TCAAGTGACT TCTCTGACCT TAATACTCAA ACAAACTGGA CCAAGTCACT TCCTTTGCCA
    ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAGACAGTCC AGGCTGATGT GGTTGCCATT
    AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGTCTTC GGCGGTCTCT AATTTACACA
    GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT
    GACAACATAC AGAAGGAGCT AGCATCAGTC ACTGGCCAAG ATGATGGTGG TGGTCACTCG
    TTGTACACCC CAGACCACTG CTCCACACTG GGAAGGCTTG ATAGCTATTG CTCTGCTGGG
    CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACTGAGG AAGTGAAAGT TGTACCCCCT
    TCAATGAGAA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GAGCCACTTC
    TCAGGCTCCT CCGGGAACAT GTCTGTGTTG AGTGATTCTG CAGGTATTGT GTTCCCTTCT
    CGCCTCAACA ATGATGCTGG CTTCCACAGT CTCCCACGTT CTGGAGCGAG GGTGAGTGTT
    CAGTCCCTCG AGTCACGATT GGGTGCCCTG AGCCCTGCAG AAGACCTGGA TGGCACTTAT
    CCCTACCAGA GTGGTCACCC ACAAGTAGAT GAAAATTCAG GACATTTGGG AGGTGCCTCA
    AGGACTGGAA TGCTTTTGAG ACCCAAGTCC CAGGAGCTGA GGCATTTTGA GAATGTGATC
    AGCCCAGCGT GTGTGGTCTC TCCTCATGCC ACTTACTCCA CCAGCATCAT CCCAAATGCC
    ACACTCTCTT CCTCTTCAGA GATTATTATT ATTCACACTG CTCAGAGTTC AGGACAGCTG
    GACAGTAAAA TCACCAGCTC CTCTTCATAC TCAAAGATAA AATCTAGAGA CCACCTCCTC
    TCCAGGCATA CTAGTAAAGA TGATCATCAG TCTCCCAGTG GCAACTGGAC AGAGGGTCAC
    CCCAGCATTC TTTCACAGGC CTTAGATCCC CACACCTCCA GTGCAACCAT GCTATTGTCC
    CTCTGTGATT CAGTGGTTTC TCTAAATACT TCAGCAAATC GGGAGAATGG GTCCCAAGCC
    ATGAGCTATA ACTGTAAAAA CAACCTGAGT TCTCCGGCCC ACGCCCAGGA TGTGGATGGC
    AAGAGTGAGT CCAGTTATTC AGGAGGCACG GGGCCCAGCA GCTCGGAGCC CTGGGAATAT
    AGTGCCCCCG GTAACGGGCA AGCATGCCCA CTGAAGGCTC ATTTGACAAC GCCTGGTTAC
    TCCACTCCCG GAAGTAATGT GAGCAGCTGC AGCTTGGACC AAACATCCAC CAAAGATGAT
    GCCAGATCCC TGTATTCAGA GGAGCATGAT GGCTACTACA CATCTGTGCA CCCCGATCCG
    GGACAGGGAT CTGGAAACCA GTACAATAGT GATGGCTTTG GGAACCCCAG GCACAGTGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCACA TTACCATGAC
    AAGTCCCTTT CTCGAAACAT CTCTTTGAAG AAAGCGAAGA AGCCGCCTCT GCCACCCTCT
    CGGACTGACT CCCTGCGCAG GATTCCCAAG AAGGGTGTCC AGTCCAATGG GCAGGCGCTG
    AACGAGAGCC TGATCGCCTC GCTGCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    AGCAGCTCGC CCTCCCAGAG CCCCTGCAGC GACTTTGAAG AGCCCTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACGGTGAG TGCGGGCAGC AGCATGACCT CGGCCACCAC CCCCAACGTC
    TACTCCCTGT GCGGGGCCAC GCCGTCGCAG AGTGACACGA GCAGCGTCAA GTCTGAGTAC
    ACAGACCCTT GGGGTTACTA CATTGACTAC ACAGGCATGC AGGAAGACCC CGGGCCCCCT
    GGCGGGGGCT GCTCAGCCAG CAGTGGGGCG CCGGCTGGAA ATGGGCCAGT CCACCACGTC
    CAGGAGGGAT CCAGGACCCA AGTGCCTGGT GGCTCAGTCA AACCAAAGAT CACGTCGCCA
    GAGAAGTCCC ACAGAGTCAC TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC
    ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA TGGGAAGGGG
    AAGACCAAGC CCAAGGTGCC AGAAAGGAAG TCCTCTCTGA TATGTTCAGT ATCCATCTCC
    TCATCGTCCA CGTCTCTGTC TTCCAATACT TCCACGGAAG GAAGCGGGAC AATGAAGAAG
    CTGGACTCGG TGCTGGCCTC CCCCACTGCG GGTGCTTCTC TCCTTTCTCT CCCCTCACCG
    TGTGCTGCTG ACAAGTCTCC TTTGCTTCCT CCTCCCCCAC CTTTAGTAGA TTCCCCTGAC
    GGCTCTCTGC CTCAGTCTCC CCTCTTCCCC CCTCCACCAC CGGAAGTTCT CACTCCCTTC
    TCTGCCCCTG CCAGCGGGTG CCTCCCTCCT TCCCCCAGGG CACTCAGCCC CCTTACTCTG
    GGTGCATCTG CTTCCCTGCC ACTGCCCCCC GCTGCCCTGC TCTCCTCAGC TCCCCCGCCT
    GCCCCACCCC TGGACCCTAC ATTGATGAAA GATGCCAGGC CTTCTTTCAA AAAACCTGGC
    CAGCCCGAGT GCTCCCGGGA GGGCTTCCGG CAGCCTTGTA ACAAGGAGGA AGGCGGTAGG
    CCCCCCATGC CCCTGATAAC CACTGAAACG TTGCAGATGG TACAGTTGAG GCCCGTGAAA
    AAGAACTCAG GAACCGAGCA AGCACTGTTA TATGAACAAG TATCTCAGGA AAAACTAACT
    CCGGTCGTCC CCCAGTATCA TTTAAAGCCA TCTGCTTTCT TGAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGCCTGCC TCTGTGACAA GCTCGCCGCC GCCTCCTGCC
    AAGAGCTCGA GTCAGGGTCA CCGGGACGGT GCAGCCGAGC ATGGTCCCCC GAGCCGCAGC
    CCGGGCGGTG CCAGGGAGGC AGAGGCTGGG CCCGGCGCCC GGCCCGCCCC GCTCCAGGAG
    CCCCCCGGCC CTTCACCCAG CAGGAAGCCA CCCCCCATTT CCAAGAAGCC CAAACTGTTT
    CTCGTGGTGC CACCTCCGCA GAGAGATCTC ACAGCGGAGC CCGCGGAGCC CGCGAGCGGA
    GCATCACCCG GCCCCAGGCG GGGCGAGGCA GCCGAGAGTG GTAGAGCCAG CGCTGGTCCT
    GATGAGCCGG GCTCTGGCAG CGCCGTGCTC GGGGGAGGAG CCGCAGGAGC CGCGGCCCCG
    GCCCCGGGTG GAGTGAAAGC CGACGTCCCC ATGGTGCAGC CCGACGTGCA GCCAGCCCCC
    AAGCAGGAGG AGCCAGGTGC CGGGACCAGT GCCGACGGCG CAGGCGACGT GGAGAGCCGC
    CTGCCTCTCC AGGACGGACG GCCTGGGCTG CCAGAGCCTG ACGCAGCCGG CTCTTCCTCG
    GAGGCCTGTG ACTTCCCCAG GGAAGAAGGG AGTGATGAGG TGATGACCCC CAGTAGACCC
    CGGACCACAG AAGACCTTTT TGCAGCTATT CACAGATCCA AGAGGAAAGT CCTGGGCCGG
    AAGGATTCAG ATGACGATCA CTCCCGAAAT CATTCTCCCT CCCCTCCGGT GACGCCCACC
    GGTGCGGCCC CCAGCCTGGC TTCCCCCAAG CAAGTGGGGT CAATTCAGAG AAGTGTCCGG
    AAGAGCAGCA CCAGCAGTGA CAACTTCAAA GCTCTGCTGC TAAAAAAGGG GAGCCGCTCG
    GACACCAGCG CCCGCATGTC TGCAGCTGAG ATGCTAAAGA ACACAGACCC TAGATTCCAG
    AGGTCAAGGT CAGAGCCTTC GCCAGATACT CCCGAGAGCC CGTCAAGCTG CTCCCCCAGC
    AAGAACAGAA GGGCCCAGGA GGAGTGGGCC AAGAACGAAG GCCTGATGCC TCGGAGTCTG
    TCCTTCTCTG GCCCCCGGTA CGGGCGCAGT CGAACTCCAC CTTCTGCAGC CAGCAGCAGG
    TACAGCATGC GGAACCGAAT CCAGAGCAGT CCCATGACTG TCATCTCAGA AGGCGAAGGG
    GAGGCCATGG AGCCTGTAGA TAGCCGGGCT CACGGGGCCC TGGGCACCGC CAGGGGGTGT
    TCCATGAATG GACTAGGTGG GGAGCCCACC ACCCCCCTGC AGGCACAGGG TCCTGGCCCT
    GCTGATGGGA TAGTCAGCGC AGAGGGCACA CATGGAACAG AACAACGTGG AGGTCCTCAG
    AGGGAAGAGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615512.1
    CDS254..4948
    Translation

    Target ORF information:

    RefSeq Version XM_005615455.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615455.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCTGCC TGAAAGCGGT TTCCAACCTA GATGAGGAGA GTAGATGGAC AGTCCACTAC 
    ACTGCTCCAT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC ACCCTGTGTA
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TATTGAGGGA ATGCGATAAA
    CTAAGACGAG ATGGCTACCG AAGTTCTCAG TACTACTCCC AGGGGCCCAC CTTTGCAGCC
    AATGCCAGCC CACTCTGTGA TGATTATCAA GATGAGGATG AAGAAACAGA TCAAAAGTGT
    TCTGTTTCTT CATCAGAAGA AGAAAGACTT ATTTCCATCA GGAGACCTAA AACGCCAACC
    TCAAGTGACT TCTCTGACCT TAATACTCAA ACAAACTGGA CCAAGTCACT TCCTTTGCCA
    ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAGACAGTCC AGGCTGATGT GGTTGCCATT
    AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGTCTTC GGCGGTCTCT AATTTACACA
    GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT
    GACAACATAC AGAAGGAGCT AGCATCAGTC ACTGGCCAAG ATGATGGTGG TGGTCACTCG
    TTGTACACCC CAGACCACTG CTCCACACTG GGAAGGCTTG ATAGCTATTG CTCTGCTGGG
    CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACTGAGG AAGTGAAAGT TGTACCCCCT
    TCAATGAGAA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GAGCCACTTC
    TCAGGCTCCT CCGGGAACAT GTCTGTGTTG AGTGATTCTG CAGGTATTGT GTTCCCTTCT
    CGCCTCAACA ATGATGCTGG CTTCCACAGT CTCCCACGTT CTGGAGCGAG GGTGAGTGTT
    CAGTCCCTCG AGTCACGATT GGGTGCCCTG AGCCCTGCAG AAGACCTGGA TGGCACTTAT
    CCCTACCAGA GTGGTCACCC ACAAGTAGAT GAAAATTCAG GACATTTGGG AGGTGCCTCA
    AGGACTGGAA TGCTTTTGAG ACCCAAGTCC CAGGAGCTGA GGCATTTTGA GAATGTGATC
    AGCCCAGCGT GTGTGGTCTC TCCTCATGCC ACTTACTCCA CCAGCATCAT CCCAAATGCC
    ACACTCTCTT CCTCTTCAGA GATTATTATT ATTCACACTG CTCAGAGTTC AGGACAGCTG
    GACAGTAAAA TCACCAGCTC CTCTTCATAC TCAAAGATAA AATCTAGAGA CCACCTCCTC
    TCCAGGCATA CTAGTAAAGA TGATCATCAG TCTCCCAGTG GCAACTGGAC AGAGGGTCAC
    CCCAGCATTC TTTCACAGGC CTTAGATCCC CACACCTCCA GTGCAACCAT GCTATTGTCC
    CTCTGTGATT CAGTGGTTTC TCTAAATACT TCAGCAAATC GGGAGAATGG GTCCCAAGCC
    ATGAGCTATA ACTGTAAAAA CAACCTGAGT TCTCCGGCCC ACGCCCAGGA TGTGGATGGC
    AAGAGTGAGT CCAGTTATTC AGGAGGCACG GGGCCCAGCA GCTCGGAGCC CTGGGAATAT
    AGTGCCCCCG GTAACGGGCA AGCATGCCCA CTGAAGGCTC ATTTGACAAC GCCTGGTTAC
    TCCACTCCCG GAAGTAATGT GAGCAGCTGC AGCTTGGACC AAACATCCAC CAAAGATGAT
    GCCAGATCCC TGTATTCAGA GGAGCATGAT GGCTACTACA CATCTGTGCA CCCCGATCCG
    GGACAGGGAT CTGGAAACCA GTACAATAGT GATGGCTTTG GGAACCCCAG GCACAGTGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCACA TTACCATGAC
    AAGTCCCTTT CTCGAAACAT CTCTTTGAAG AAAGCGAAGA AGCCGCCTCT GCCACCCTCT
    CGGACTGACT CCCTGCGCAG GATTCCCAAG AAGGGTGTCC AGTCCAATGG GCAGGCGCTG
    AACGAGAGCC TGATCGCCTC GCTGCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    AGCAGCTCGC CCTCCCAGAG CCCCTGCAGC GACTTTGAAG AGCCCTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACGGTGAG TGCGGGCAGC AGCATGACCT CGGCCACCAC CCCCAACGTC
    TACTCCCTGT GCGGGGCCAC GCCGTCGCAG AGTGACACGA GCAGCGTCAA GTCTGAGTAC
    ACAGACCCTT GGGGTTACTA CATTGACTAC ACAGGCATGC AGGAAGACCC CGGGCCCCCT
    GGCGGGGGCT GCTCAGCCAG CAGTGGGGCG CCGGCTGGAA ATGGGCCAGT CCACCACGTC
    CAGGAGGGAT CCAGGACCCA AGTGCCTGGT GGCTCAGTCA AACCAAAGAT CACGTCGCCA
    GAGAAGTCCC ACAGAGTCAC TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC
    ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA TGGGAAGGGG
    AAGACCAAGC CCAAGGTGCC AGAAAGGAAG TCCTCTCTGA TATGTTCAGT ATCCATCTCC
    TCATCGTCCA CGTCTCTGTC TTCCAATACT TCCACGGAAG GAAGCGGGAC AATGAAGAAG
    CTGGACTCGG TGCTGGCCTC CCCCACTGCG GGTGCTTCTC TCCTTTCTCT CCCCTCACCG
    TGTGCTGCTG ACAAGTCTCC TTTGCTTCCT CCTCCCCCAC CTTTAGTAGA TTCCCCTGAC
    GGCTCTCTGC CTCAGTCTCC CCTCTTCCCC CCTCCACCAC CGGAAGTTCT CACTCCCTTC
    TCTGCCCCTG CCAGCGGGTG CCTCCCTCCT TCCCCCAGGG CACTCAGCCC CCTTACTCTG
    GGTGCATCTG CTTCCCTGCC ACTGCCCCCC GCTGCCCTGC TCTCCTCAGC TCCCCCGCCT
    GCCCCACCCC TGGACCCTAC ATTGATGAAA GATGCCAGGC CTTCTTTCAA AAAACCTGGC
    CAGCCCGAGT GCTCCCGGGA GGGCTTCCGG CAGCCTTGTA ACAAGGAGGA AGGCGGTAGG
    CCCCCCATGC CCCTGATAAC CACTGAAACG TTGCAGATGG TACAGTTGAG GCCCGTGAAA
    AAGAACTCAG GAACCGAGCA AGCACTGTTA TATGAACAAG TATCTCAGGA AAAACTAACT
    CCGGTCGTCC CCCAGTATCA TTTAAAGCCA TCTGCTTTCT TGAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGCCTGCC TCTGTGACAA GCTCGCCGCC GCCTCCTGCC
    AAGAGCTCGA GTCAGGGTCA CCGGGACGGT GCAGCCGAGC ATGGTCCCCC GAGCCGCAGC
    CCGGGCGGTG CCAGGGAGGC AGAGGCTGGG CCCGGCGCCC GGCCCGCCCC GCTCCAGGAG
    CCCCCCGGCC CTTCACCCAG CAGGAAGCCA CCCCCCATTT CCAAGAAGCC CAAACTGTTT
    CTCGTGGTGC CACCTCCGCA GAGAGATCTC ACAGCGGAGC CCGCGGAGCC CGCGAGCGGA
    GCATCACCCG GCCCCAGGCG GGGCGAGGCA GCCGAGAGTG GTAGAGCCAG CGCTGGTCCT
    GATGAGCCGG GCTCTGGCAG CGCCGTGCTC GGGGGAGGAG CCGCAGGAGC CGCGGCCCCG
    GCCCCGGGTG GAGTGAAAGC CGACGTCCCC ATGGTGCAGC CCGACGTGCA GCCAGCCCCC
    AAGCAGGAGG AGCCAGGTGC CGGGACCAGT GCCGACGGCG CAGGCGACGT GGAGAGCCGC
    CTGCCTCTCC AGGACGGACG GCCTGGGCTG CCAGAGCCTG ACGCAGCCGG CTCTTCCTCG
    GAGGCCTGTG ACTTCCCCAG GGAAGAAGGG AGTGATGAGG TGATGACCCC CAGTAGACCC
    CGGACCACAG AAGACCTTTT TGCAGCTATT CACAGATCCA AGAGGAAAGT CCTGGGCCGG
    AAGGATTCAG ATGACGATCA CTCCCGAAAT CATTCTCCCT CCCCTCCGGT GACGCCCACC
    GGTGCGGCCC CCAGCCTGGC TTCCCCCAAG CAAGTGGGGT CAATTCAGAG AAGTGTCCGG
    AAGAGCAGCA CCAGCAGTGA CAACTTCAAA GCTCTGCTGC TAAAAAAGGG GAGCCGCTCG
    GACACCAGCG CCCGCATGTC TGCAGCTGAG ATGCTAAAGA ACACAGACCC TAGATTCCAG
    AGGTCAAGGT CAGAGCCTTC GCCAGATACT CCCGAGAGCC CGTCAAGCTG CTCCCCCAGC
    AAGAACAGAA GGGCCCAGGA GGAGTGGGCC AAGAACGAAG GCCTGATGCC TCGGAGTCTG
    TCCTTCTCTG GCCCCCGGTA CGGGCGCAGT CGAACTCCAC CTTCTGCAGC CAGCAGCAGG
    TACAGCATGC GGAACCGAAT CCAGAGCAGT CCCATGACTG TCATCTCAGA AGGCGAAGGG
    GAGGCCATGG AGCCTGTAGA TAGCCGGGCT CACGGGGCCC TGGGCACCGC CAGGGGGTGT
    TCCATGAATG GACTAGGTGG GGAGCCCACC ACCCCCCTGC AGGCACAGGG TCCTGGCCCT
    GCTGATGGGA TAGTCAGCGC AGAGGGCACA CATGGAACAG AACAACGTGG AGGTCCTCAG
    AGGGAAGAGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00878
    Clone ID Related Accession (Same CDS sequence) XM_005615455.2 , XM_005615455.3
    Accession Version XM_005615455.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615455.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCTGCC TGAAAGCGGT TTCCAACCTA GATGAGGAGA GTAGATGGAC AGTCCACTAC 
    ACTGCTCCAT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC ACCCTGTGTA
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TATTGAGGGA ATGCGATAAA
    CTAAGACGAG ATGGCTACCG AAGTTCTCAG TACTACTCCC AGGGGCCCAC CTTTGCAGCC
    AATGCCAGCC CACTCTGTGA TGATTATCAA GATGAGGATG AAGAAACAGA TCAAAAGTGT
    TCTGTTTCTT CATCAGAAGA AGAAAGACTT ATTTCCATCA GGAGACCTAA AACGCCAACC
    TCAAGTGACT TCTCTGACCT TAATACTCAA ACAAACTGGA CCAAGTCACT TCCTTTGCCA
    ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAGACAGTCC AGGCTGATGT GGTTGCCATT
    AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGTCTTC GGCGGTCTCT AATTTACACA
    GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT
    GACAACATAC AGAAGGAGCT AGCATCAGTC ACTGGCCAAG ATGATGGTGG TGGTCACTCG
    TTGTACACCC CAGACCACTG CTCCACACTG GGAAGGCTTG ATAGCTATTG CTCTGCTGGG
    CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACTGAGG AAGTGAAAGT TGTACCCCCT
    TCAATGAGAA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GAGCCACTTC
    TCAGGCTCCT CCGGGAACAT GTCTGTGTTG AGTGATTCTG CAGGTATTGT GTTCCCTTCT
    CGCCTCAACA ATGATGCTGG CTTCCACAGT CTCCCACGTT CTGGAGCGAG GGTGAGTGTT
    CAGTCCCTCG AGTCACGATT GGGTGCCCTG AGCCCTGCAG AAGACCTGGA TGGCACTTAT
    CCCTACCAGA GTGGTCACCC ACAAGTAGAT GAAAATTCAG GACATTTGGG AGGTGCCTCA
    AGGACTGGAA TGCTTTTGAG ACCCAAGTCC CAGGAGCTGA GGCATTTTGA GAATGTGATC
    AGCCCAGCGT GTGTGGTCTC TCCTCATGCC ACTTACTCCA CCAGCATCAT CCCAAATGCC
    ACACTCTCTT CCTCTTCAGA GATTATTATT ATTCACACTG CTCAGAGTTC AGGACAGCTG
    GACAGTAAAA TCACCAGCTC CTCTTCATAC TCAAAGATAA AATCTAGAGA CCACCTCCTC
    TCCAGGCATA CTAGTAAAGA TGATCATCAG TCTCCCAGTG GCAACTGGAC AGAGGGTCAC
    CCCAGCATTC TTTCACAGGC CTTAGATCCC CACACCTCCA GTGCAACCAT GCTATTGTCC
    CTCTGTGATT CAGTGGTTTC TCTAAATACT TCAGCAAATC GGGAGAATGG GTCCCAAGCC
    ATGAGCTATA ACTGTAAAAA CAACCTGAGT TCTCCGGCCC ACGCCCAGGA TGTGGATGGC
    AAGAGTGAGT CCAGTTATTC AGGAGGCACG GGGCCCAGCA GCTCGGAGCC CTGGGAATAT
    AGTGCCCCCG GTAACGGGCA AGCATGCCCA CTGAAGGCTC ATTTGACAAC GCCTGGTTAC
    TCCACTCCCG GAAGTAATGT GAGCAGCTGC AGCTTGGACC AAACATCCAC CAAAGATGAT
    GCCAGATCCC TGTATTCAGA GGAGCATGAT GGCTACTACA CATCTGTGCA CCCCGATCCG
    GGACAGGGAT CTGGAAACCA GTACAATAGT GATGGCTTTG GGAACCCCAG GCACAGTGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCACA TTACCATGAC
    AAGTCCCTTT CTCGAAACAT CTCTTTGAAG AAAGCGAAGA AGCCGCCTCT GCCACCCTCT
    CGGACTGACT CCCTGCGCAG GATTCCCAAG AAGGGTGTCC AGTCCAATGG GCAGGCGCTG
    AACGAGAGCC TGATCGCCTC GCTGCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    AGCAGCTCGC CCTCCCAGAG CCCCTGCAGC GACTTTGAAG AGCCCTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACGGTGAG TGCGGGCAGC AGCATGACCT CGGCCACCAC CCCCAACGTC
    TACTCCCTGT GCGGGGCCAC GCCGTCGCAG AGTGACACGA GCAGCGTCAA GTCTGAGTAC
    ACAGACCCTT GGGGTTACTA CATTGACTAC ACAGGCATGC AGGAAGACCC CGGGCCCCCT
    GGCGGGGGCT GCTCAGCCAG CAGTGGGGCG CCGGCTGGAA ATGGGCCAGT CCACCACGTC
    CAGGAGGGAT CCAGGACCCA AGTGCCTGGT GGCTCAGTCA AACCAAAGAT CACGTCGCCA
    GAGAAGTCCC ACAGAGTCAC TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC
    ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA TGGGAAGGGG
    AAGACCAAGC CCAAGGTGCC AGAAAGGAAG TCCTCTCTGA TATGTTCAGT ATCCATCTCC
    TCATCGTCCA CGTCTCTGTC TTCCAATACT TCCACGGAAG GAAGCGGGAC AATGAAGAAG
    CTGGACTCGG TGCTGGCCTC CCCCACTGCG GGTGCTTCTC TCCTTTCTCT CCCCTCACCG
    TGTGCTGCTG ACAAGTCTCC TTTGCTTCCT CCTCCCCCAC CTTTAGTAGA TTCCCCTGAC
    GGCTCTCTGC CTCAGTCTCC CCTCTTCCCC CCTCCACCAC CGGAAGTTCT CACTCCCTTC
    TCTGCCCCTG CCAGCGGGTG CCTCCCTCCT TCCCCCAGGG CACTCAGCCC CCTTACTCTG
    GGTGCATCTG CTTCCCTGCC ACTGCCCCCC GCTGCCCTGC TCTCCTCAGC TCCCCCGCCT
    GCCCCACCCC TGGACCCTAC ATTGATGAAA GATGCCAGGC CTTCTTTCAA AAAACCTGGC
    CAGCCCGAGT GCTCCCGGGA GGGCTTCCGG CAGCCTTGTA ACAAGGAGGA AGGCGGTAGG
    CCCCCCATGC CCCTGATAAC CACTGAAACG TTGCAGATGG TACAGTTGAG GCCCGTGAAA
    AAGAACTCAG GAACCGAGCA AGCACTGTTA TATGAACAAG TATCTCAGGA AAAACTAACT
    CCGGTCGTCC CCCAGTATCA TTTAAAGCCA TCTGCTTTCT TGAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGCCTGCC TCTGTGACAA GCTCGCCGCC GCCTCCTGCC
    AAGAGCTCGA GTCAGGGTCA CCGGGACGGT GCAGCCGAGC ATGGTCCCCC GAGCCGCAGC
    CCGGGCGGTG CCAGGGAGGC AGAGGCTGGG CCCGGCGCCC GGCCCGCCCC GCTCCAGGAG
    CCCCCCGGCC CTTCACCCAG CAGGAAGCCA CCCCCCATTT CCAAGAAGCC CAAACTGTTT
    CTCGTGGTGC CACCTCCGCA GAGAGATCTC ACAGCGGAGC CCGCGGAGCC CGCGAGCGGA
    GCATCACCCG GCCCCAGGCG GGGCGAGGCA GCCGAGAGTG GTAGAGCCAG CGCTGGTCCT
    GATGAGCCGG GCTCTGGCAG CGCCGTGCTC GGGGGAGGAG CCGCAGGAGC CGCGGCCCCG
    GCCCCGGGTG GAGTGAAAGC CGACGTCCCC ATGGTGCAGC CCGACGTGCA GCCAGCCCCC
    AAGCAGGAGG AGCCAGGTGC CGGGACCAGT GCCGACGGCG CAGGCGACGT GGAGAGCCGC
    CTGCCTCTCC AGGACGGACG GCCTGGGCTG CCAGAGCCTG ACGCAGCCGG CTCTTCCTCG
    GAGGCCTGTG ACTTCCCCAG GGAAGAAGGG AGTGATGAGG TGATGACCCC CAGTAGACCC
    CGGACCACAG AAGACCTTTT TGCAGCTATT CACAGATCCA AGAGGAAAGT CCTGGGCCGG
    AAGGATTCAG ATGACGATCA CTCCCGAAAT CATTCTCCCT CCCCTCCGGT GACGCCCACC
    GGTGCGGCCC CCAGCCTGGC TTCCCCCAAG CAAGTGGGGT CAATTCAGAG AAGTGTCCGG
    AAGAGCAGCA CCAGCAGTGA CAACTTCAAA GCTCTGCTGC TAAAAAAGGG GAGCCGCTCG
    GACACCAGCG CCCGCATGTC TGCAGCTGAG ATGCTAAAGA ACACAGACCC TAGATTCCAG
    AGGTCAAGGT CAGAGCCTTC GCCAGATACT CCCGAGAGCC CGTCAAGCTG CTCCCCCAGC
    AAGAACAGAA GGGCCCAGGA GGAGTGGGCC AAGAACGAAG GCCTGATGCC TCGGAGTCTG
    TCCTTCTCTG GCCCCCGGTA CGGGCGCAGT CGAACTCCAC CTTCTGCAGC CAGCAGCAGG
    TACAGCATGC GGAACCGAAT CCAGAGCAGT CCCATGACTG TCATCTCAGA AGGCGAAGGG
    GAGGCCATGG AGCCTGTAGA TAGCCGGGCT CACGGGGCCC TGGGCACCGC CAGGGGGTGT
    TCCATGAATG GACTAGGTGG GGAGCCCACC ACCCCCCTGC AGGCACAGGG TCCTGGCCCT
    GCTGATGGGA TAGTCAGCGC AGAGGGCACA CATGGAACAG AACAACGTGG AGGTCCTCAG
    AGGGAAGAGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615512.1
    CDS286..4980
    Translation

    Target ORF information:

    RefSeq Version XM_005615455.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615455.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCTGCC TGAAAGCGGT TTCCAACCTA GATGAGGAGA GTAGATGGAC AGTCCACTAC 
    ACTGCTCCAT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC ACCCTGTGTA
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TATTGAGGGA ATGCGATAAA
    CTAAGACGAG ATGGCTACCG AAGTTCTCAG TACTACTCCC AGGGGCCCAC CTTTGCAGCC
    AATGCCAGCC CACTCTGTGA TGATTATCAA GATGAGGATG AAGAAACAGA TCAAAAGTGT
    TCTGTTTCTT CATCAGAAGA AGAAAGACTT ATTTCCATCA GGAGACCTAA AACGCCAACC
    TCAAGTGACT TCTCTGACCT TAATACTCAA ACAAACTGGA CCAAGTCACT TCCTTTGCCA
    ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAGACAGTCC AGGCTGATGT GGTTGCCATT
    AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGTCTTC GGCGGTCTCT AATTTACACA
    GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT
    GACAACATAC AGAAGGAGCT AGCATCAGTC ACTGGCCAAG ATGATGGTGG TGGTCACTCG
    TTGTACACCC CAGACCACTG CTCCACACTG GGAAGGCTTG ATAGCTATTG CTCTGCTGGG
    CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACTGAGG AAGTGAAAGT TGTACCCCCT
    TCAATGAGAA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GAGCCACTTC
    TCAGGCTCCT CCGGGAACAT GTCTGTGTTG AGTGATTCTG CAGGTATTGT GTTCCCTTCT
    CGCCTCAACA ATGATGCTGG CTTCCACAGT CTCCCACGTT CTGGAGCGAG GGTGAGTGTT
    CAGTCCCTCG AGTCACGATT GGGTGCCCTG AGCCCTGCAG AAGACCTGGA TGGCACTTAT
    CCCTACCAGA GTGGTCACCC ACAAGTAGAT GAAAATTCAG GACATTTGGG AGGTGCCTCA
    AGGACTGGAA TGCTTTTGAG ACCCAAGTCC CAGGAGCTGA GGCATTTTGA GAATGTGATC
    AGCCCAGCGT GTGTGGTCTC TCCTCATGCC ACTTACTCCA CCAGCATCAT CCCAAATGCC
    ACACTCTCTT CCTCTTCAGA GATTATTATT ATTCACACTG CTCAGAGTTC AGGACAGCTG
    GACAGTAAAA TCACCAGCTC CTCTTCATAC TCAAAGATAA AATCTAGAGA CCACCTCCTC
    TCCAGGCATA CTAGTAAAGA TGATCATCAG TCTCCCAGTG GCAACTGGAC AGAGGGTCAC
    CCCAGCATTC TTTCACAGGC CTTAGATCCC CACACCTCCA GTGCAACCAT GCTATTGTCC
    CTCTGTGATT CAGTGGTTTC TCTAAATACT TCAGCAAATC GGGAGAATGG GTCCCAAGCC
    ATGAGCTATA ACTGTAAAAA CAACCTGAGT TCTCCGGCCC ACGCCCAGGA TGTGGATGGC
    AAGAGTGAGT CCAGTTATTC AGGAGGCACG GGGCCCAGCA GCTCGGAGCC CTGGGAATAT
    AGTGCCCCCG GTAACGGGCA AGCATGCCCA CTGAAGGCTC ATTTGACAAC GCCTGGTTAC
    TCCACTCCCG GAAGTAATGT GAGCAGCTGC AGCTTGGACC AAACATCCAC CAAAGATGAT
    GCCAGATCCC TGTATTCAGA GGAGCATGAT GGCTACTACA CATCTGTGCA CCCCGATCCG
    GGACAGGGAT CTGGAAACCA GTACAATAGT GATGGCTTTG GGAACCCCAG GCACAGTGTG
    GTCAATGTTT TTGATGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCACA TTACCATGAC
    AAGTCCCTTT CTCGAAACAT CTCTTTGAAG AAAGCGAAGA AGCCGCCTCT GCCACCCTCT
    CGGACTGACT CCCTGCGCAG GATTCCCAAG AAGGGTGTCC AGTCCAATGG GCAGGCGCTG
    AACGAGAGCC TGATCGCCTC GCTGCAGCAC TCGCTGCAGC TGAACCTCCC GGGCAAGGGC
    AGCAGCTCGC CCTCCCAGAG CCCCTGCAGC GACTTTGAAG AGCCCTGGCT GCCTCGCTCC
    CGCAGCCAGA GCACGGTGAG TGCGGGCAGC AGCATGACCT CGGCCACCAC CCCCAACGTC
    TACTCCCTGT GCGGGGCCAC GCCGTCGCAG AGTGACACGA GCAGCGTCAA GTCTGAGTAC
    ACAGACCCTT GGGGTTACTA CATTGACTAC ACAGGCATGC AGGAAGACCC CGGGCCCCCT
    GGCGGGGGCT GCTCAGCCAG CAGTGGGGCG CCGGCTGGAA ATGGGCCAGT CCACCACGTC
    CAGGAGGGAT CCAGGACCCA AGTGCCTGGT GGCTCAGTCA AACCAAAGAT CACGTCGCCA
    GAGAAGTCCC ACAGAGTCAC TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC
    ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA TGGGAAGGGG
    AAGACCAAGC CCAAGGTGCC AGAAAGGAAG TCCTCTCTGA TATGTTCAGT ATCCATCTCC
    TCATCGTCCA CGTCTCTGTC TTCCAATACT TCCACGGAAG GAAGCGGGAC AATGAAGAAG
    CTGGACTCGG TGCTGGCCTC CCCCACTGCG GGTGCTTCTC TCCTTTCTCT CCCCTCACCG
    TGTGCTGCTG ACAAGTCTCC TTTGCTTCCT CCTCCCCCAC CTTTAGTAGA TTCCCCTGAC
    GGCTCTCTGC CTCAGTCTCC CCTCTTCCCC CCTCCACCAC CGGAAGTTCT CACTCCCTTC
    TCTGCCCCTG CCAGCGGGTG CCTCCCTCCT TCCCCCAGGG CACTCAGCCC CCTTACTCTG
    GGTGCATCTG CTTCCCTGCC ACTGCCCCCC GCTGCCCTGC TCTCCTCAGC TCCCCCGCCT
    GCCCCACCCC TGGACCCTAC ATTGATGAAA GATGCCAGGC CTTCTTTCAA AAAACCTGGC
    CAGCCCGAGT GCTCCCGGGA GGGCTTCCGG CAGCCTTGTA ACAAGGAGGA AGGCGGTAGG
    CCCCCCATGC CCCTGATAAC CACTGAAACG TTGCAGATGG TACAGTTGAG GCCCGTGAAA
    AAGAACTCAG GAACCGAGCA AGCACTGTTA TATGAACAAG TATCTCAGGA AAAACTAACT
    CCGGTCGTCC CCCAGTATCA TTTAAAGCCA TCTGCTTTCT TGAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGCCTGCC TCTGTGACAA GCTCGCCGCC GCCTCCTGCC
    AAGAGCTCGA GTCAGGGTCA CCGGGACGGT GCAGCCGAGC ATGGTCCCCC GAGCCGCAGC
    CCGGGCGGTG CCAGGGAGGC AGAGGCTGGG CCCGGCGCCC GGCCCGCCCC GCTCCAGGAG
    CCCCCCGGCC CTTCACCCAG CAGGAAGCCA CCCCCCATTT CCAAGAAGCC CAAACTGTTT
    CTCGTGGTGC CACCTCCGCA GAGAGATCTC ACAGCGGAGC CCGCGGAGCC CGCGAGCGGA
    GCATCACCCG GCCCCAGGCG GGGCGAGGCA GCCGAGAGTG GTAGAGCCAG CGCTGGTCCT
    GATGAGCCGG GCTCTGGCAG CGCCGTGCTC GGGGGAGGAG CCGCAGGAGC CGCGGCCCCG
    GCCCCGGGTG GAGTGAAAGC CGACGTCCCC ATGGTGCAGC CCGACGTGCA GCCAGCCCCC
    AAGCAGGAGG AGCCAGGTGC CGGGACCAGT GCCGACGGCG CAGGCGACGT GGAGAGCCGC
    CTGCCTCTCC AGGACGGACG GCCTGGGCTG CCAGAGCCTG ACGCAGCCGG CTCTTCCTCG
    GAGGCCTGTG ACTTCCCCAG GGAAGAAGGG AGTGATGAGG TGATGACCCC CAGTAGACCC
    CGGACCACAG AAGACCTTTT TGCAGCTATT CACAGATCCA AGAGGAAAGT CCTGGGCCGG
    AAGGATTCAG ATGACGATCA CTCCCGAAAT CATTCTCCCT CCCCTCCGGT GACGCCCACC
    GGTGCGGCCC CCAGCCTGGC TTCCCCCAAG CAAGTGGGGT CAATTCAGAG AAGTGTCCGG
    AAGAGCAGCA CCAGCAGTGA CAACTTCAAA GCTCTGCTGC TAAAAAAGGG GAGCCGCTCG
    GACACCAGCG CCCGCATGTC TGCAGCTGAG ATGCTAAAGA ACACAGACCC TAGATTCCAG
    AGGTCAAGGT CAGAGCCTTC GCCAGATACT CCCGAGAGCC CGTCAAGCTG CTCCCCCAGC
    AAGAACAGAA GGGCCCAGGA GGAGTGGGCC AAGAACGAAG GCCTGATGCC TCGGAGTCTG
    TCCTTCTCTG GCCCCCGGTA CGGGCGCAGT CGAACTCCAC CTTCTGCAGC CAGCAGCAGG
    TACAGCATGC GGAACCGAAT CCAGAGCAGT CCCATGACTG TCATCTCAGA AGGCGAAGGG
    GAGGCCATGG AGCCTGTAGA TAGCCGGGCT CACGGGGCCC TGGGCACCGC CAGGGGGTGT
    TCCATGAATG GACTAGGTGG GGAGCCCACC ACCCCCCTGC AGGCACAGGG TCCTGGCCCT
    GCTGATGGGA TAGTCAGCGC AGAGGGCACA CATGGAACAG AACAACGTGG AGGTCCTCAG
    AGGGAAGAGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285859
    Clone ID Related Accession (Same CDS sequence) XM_022417481.1 , XM_022417475.1
    Accession Version XM_022417481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCACCAC CATCAAGCAG GCACAAAGCG GTTTCCAACC TAGATGAGGA GAGTAGATGG 
    ACAGTCCACT ACACTGCTCC ATGGCACCAG CAGGAGAATG TGTTCCTTCC CACCACAAGA
    CCACCCTGTG TAGAGGACCT GCACCGCCAA GCCAAGCTCA ACCTCAAATC AGTATTGAGG
    GAATGCGATA AACTAAGACG AGATGGCTAC CGAAGTTCTC AGTACTACTC CCAGGGGCCC
    ACCTTTGCAG CCAATGCCAG CCCACTCTGT GATGATTATC AAGATGAGGA TGAAGAAACA
    GATCAAAAGT GTTCTGTTTC TTCATCAGAA GAAGAAAGAC TTATTTCCAT CAGGAGACCT
    AAAACGCCAA CCTCAAGTGA CTTCTCTGAC CTTAATACTC AAACAAACTG GACCAAGTCA
    CTTCCTTTGC CAACACCAGA AGAGAAGATG CGACAGCAAG CCCAGACAGT CCAGGCTGAT
    GTGGTTGCCA TTAACATAAC TGGGGAGAAT TTCGATCGCC AGGCCAGTCT TCGGCGGTCT
    CTAATTTACA CAGACACTCT GGTAAGACGA CCGAAGAAAG TCAAAAGGAG AAAGACTATT
    ACAGGAGTCC CTGACAACAT ACAGAAGGAG CTAGCATCAG TCACTGGCCA AGATGATGGT
    GGTGGTCACT CGTTGTACAC CCCAGACCAC TGCTCCACAC TGGGAAGGCT TGATAGCTAT
    TGCTCTGCTG GGCAGCGCTC AGAAACCAGG GACTCCAGCT GTCAGACTGA GGAAGTGAAA
    GTTGTACCCC CTTCAATGAG AAGAATCAGG GCACAGAAGG GGCAAGGCAT TGCTGCCCAG
    ATGAGCCACT TCTCAGGCTC CTCCGGGAAC ATGTCTGTGT TGAGTGATTC TGCAGGTATT
    GTGTTCCCTT CTCGCCTCAA CAATGATGCT GGCTTCCACA GTCTCCCACG TTCTGGAGCG
    AGGGTGAGTG TTCAGTCCCT CGAGTCACGA TTGGGTGCCC TGAGCCCTGC AGAAGACCTG
    GATGGCACTT ATCCCTACCA GAGTGGTCAC CCACAAGTAG ATGAAAATTC AGGACATTTG
    GGAGGTGCCT CAAGGACTGG AATGCTTTTG AGACCCAAGT CCCAGGAGCT GAGGCATTTT
    GAGAATGTGA TCAGCCCAGC GTGTGTGGTC TCTCCTCATG CCACTTACTC CACCAGCATC
    ATCCCAAATG CCACACTCTC TTCCTCTTCA GAGATTATTA TTATTCACAC TGCTCAGAGT
    TCAGGACAGC TGGACAGTAA AATCACCAGC TCCTCTTCAT ACTCAAAGAT AAAATCTAGA
    GACCACCTCC TCTCCAGGCA TACTAGTAAA GATGATCATC AGTCTCCCAG TGGCAACTGG
    ACAGAGGGTC ACCCCAGCAT TCTTTCACAG GCCTTAGATC CCCACACCTC CAGTGCAACC
    ATGCTATTGT CCCTCTGTGA TTCAGTGGTT TCTCTAAATA CTTCAGCAAA TCGGGAGAAT
    GGGTCCCAAG CCATGAGCTA TAACTGTAAA AACAACCTGA GTTCTCCGGC CCACGCCCAG
    GATGTGGATG GCAAGAGTGA GTCCAGTTAT TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG
    CCCTGGGAAT ATAGTGCCCC CGGTAACGGG CAAGCATGCC CACTGAAGGC TCATTTGACA
    ACGCCTGGTT ACTCCACTCC CGGAAGTAAT GTGAGCAGCT GCAGCTTGGA CCAAACATCC
    ACCAAAGATG ATGCCAGATC CCTGTATTCA GAGGAGCATG ATGGCTACTA CACATCTGTG
    CACCCCGATC CGGGACAGGG ATCTGGAAAC CAGTACAATA GTGATGGCTT TGGGAACCCC
    AGGCACAGTG TGGTCAATGT TTTTGATGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCA
    CATTACCATG ACAAGTCCCT TTCTCGAAAC ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT
    CTGCCACCCT CTCGGACTGA CTCCCTGCGC AGGATTCCCA AGAAGGGTGT CCAGTCCAAT
    GGGCAGGCGC TGAACGAGAG CCTGATCGCC TCGCTGCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG AGCCCCTGCA GCGACTTTGA AGAGCCCTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACGGTG AGTGCGGGCA GCAGCATGAC CTCGGCCACC
    ACCCCCAACG TCTACTCCCT GTGCGGGGCC ACGCCGTCGC AGAGTGACAC GAGCAGCGTC
    AAGTCTGAGT ACACAGACCC TTGGGGTTAC TACATTGACT ACACAGGCAT GCAGGAAGAC
    CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC AGCAGTGGGG CGCCGGCTGG AAATGGGCCA
    GTCCACCACG TCCAGGAGGG ATCCAGGACC CAAGTGCCTG GTGGCTCAGT CAAACCAAAG
    ATCACGTCGC CAGAGAAGTC CCACAGAGTC ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG
    TCGAATACAC CCACAGCACT CACCCCTGTG CCTGTGTTTT TAAAATCAGT GTCACCAGCA
    AATGGGAAGG GGAAGACCAA GCCCAAGGTG CCAGAAAGGA AGTCCTCTCT GATATGTTCA
    GTATCCATCT CCTCATCGTC CACGTCTCTG TCTTCCAATA CTTCCACGGA AGGAAGCGGG
    ACAATGAAGA AGCTGGACTC GGTGCTGGCC TCCCCCACTG CGGGTGCTTC TCTCCTTTCT
    CTCCCCTCAC CGTGTGCTGC TGACAAGTCT CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA
    GATTCCCCTG ACGGCTCTCT GCCTCAGTCT CCCCTCTTCC CCCCTCCACC ACCGGAAGTT
    CTCACTCCCT TCTCTGCCCC TGCCAGCGGG TGCCTCCCTC CTTCCCCCAG GGCACTCAGC
    CCCCTTACTC TGGGTGCATC TGCTTCCCTG CCACTGCCCC CCGCTGCCCT GCTCTCCTCA
    GCTCCCCCGC CTGCCCCACC CCTGGACCCT ACATTGATGA AAGATGCCAG GCCTTCTTTC
    AAAAAACCTG GCCAGCCCGA GTGCTCCCGG GAGGGCTTCC GGCAGCCTTG TAACAAGGAG
    GAAGGCGGTA GGCCCCCCAT GCCCCTGATA ACCACTGAAA CGTTGCAGAT GGTACAGTTG
    AGGCCCGTGA AAAAGAACTC AGGAACCGAG CAAGCACTGT TATATGAACA AGTATCTCAG
    GAAAAACTAA CTCCGGTCGT CCCCCAGTAT CATTTAAAGC CATCTGCTTT CTTGAAATCC
    CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG
    CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT CACCGGGACG GTGCAGCCGA GCATGGTCCC
    CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC
    CCGCTCCAGG AGCCCCCCGG CCCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG
    CCCAAACTGT TTCTCGTGGT GCCACCTCCG CAGAGAGATC TCACAGCGGA GCCCGCGGAG
    CCCGCGAGCG GAGCATCACC CGGCCCCAGG CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC
    AGCGCTGGTC CTGATGAGCC GGGCTCTGGC AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA
    GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA GCCGACGTCC CCATGGTGCA GCCCGACGTG
    CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT GCCGGGACCA GTGCCGACGG CGCAGGCGAC
    GTGGAGAGCC GCCTGCCTCT CCAGGACGGA CGGCCTGGGC TGCCAGAGCC TGACGCAGCC
    GGCTCTTCCT CGGAGGCCTG TGACTTCCCC AGGGAAGAAG GGAGTGATGA GGTGATGACC
    CCCAGTAGAC CCCGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGATC CAAGAGGAAA
    GTCCTGGGCC GGAAGGATTC AGATGACGAT CACTCCCGAA ATCATTCTCC CTCCCCTCCG
    GTGACGCCCA CCGGTGCGGC CCCCAGCCTG GCTTCCCCCA AGCAAGTGGG GTCAATTCAG
    AGAAGTGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTAAAAAAG
    GGGAGCCGCT CGGACACCAG CGCCCGCATG TCTGCAGCTG AGATGCTAAA GAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCAGAGCCT TCGCCAGATA CTCCCGAGAG CCCGTCAAGC
    TGCTCCCCCA GCAAGAACAG AAGGGCCCAG GAGGAGTGGG CCAAGAACGA AGGCCTGATG
    CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG TACGGGCGCA GTCGAACTCC ACCTTCTGCA
    GCCAGCAGCA GGTACAGCAT GCGGAACCGA ATCCAGAGCA GTCCCATGAC TGTCATCTCA
    GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA GATAGCCGGG CTCACGGGGC CCTGGGCACC
    GCCAGGGGGT GTTCCATGAA TGGACTAGGT GGGGAGCCCA CCACCCCCCT GCAGGCACAG
    GGTCCTGGCC CTGCTGATGG GATAGTCAGC GCAGAGGGCA CACATGGAAC AGAACAACGT
    GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273189.1
    CDS507..5213
    Translation

    Target ORF information:

    RefSeq Version XM_022417481.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCACCAC CATCAAGCAG GCACAAAGCG GTTTCCAACC TAGATGAGGA GAGTAGATGG 
    ACAGTCCACT ACACTGCTCC ATGGCACCAG CAGGAGAATG TGTTCCTTCC CACCACAAGA
    CCACCCTGTG TAGAGGACCT GCACCGCCAA GCCAAGCTCA ACCTCAAATC AGTATTGAGG
    GAATGCGATA AACTAAGACG AGATGGCTAC CGAAGTTCTC AGTACTACTC CCAGGGGCCC
    ACCTTTGCAG CCAATGCCAG CCCACTCTGT GATGATTATC AAGATGAGGA TGAAGAAACA
    GATCAAAAGT GTTCTGTTTC TTCATCAGAA GAAGAAAGAC TTATTTCCAT CAGGAGACCT
    AAAACGCCAA CCTCAAGTGA CTTCTCTGAC CTTAATACTC AAACAAACTG GACCAAGTCA
    CTTCCTTTGC CAACACCAGA AGAGAAGATG CGACAGCAAG CCCAGACAGT CCAGGCTGAT
    GTGGTTGCCA TTAACATAAC TGGGGAGAAT TTCGATCGCC AGGCCAGTCT TCGGCGGTCT
    CTAATTTACA CAGACACTCT GGTAAGACGA CCGAAGAAAG TCAAAAGGAG AAAGACTATT
    ACAGGAGTCC CTGACAACAT ACAGAAGGAG CTAGCATCAG TCACTGGCCA AGATGATGGT
    GGTGGTCACT CGTTGTACAC CCCAGACCAC TGCTCCACAC TGGGAAGGCT TGATAGCTAT
    TGCTCTGCTG GGCAGCGCTC AGAAACCAGG GACTCCAGCT GTCAGACTGA GGAAGTGAAA
    GTTGTACCCC CTTCAATGAG AAGAATCAGG GCACAGAAGG GGCAAGGCAT TGCTGCCCAG
    ATGAGCCACT TCTCAGGCTC CTCCGGGAAC ATGTCTGTGT TGAGTGATTC TGCAGGTATT
    GTGTTCCCTT CTCGCCTCAA CAATGATGCT GGCTTCCACA GTCTCCCACG TTCTGGAGCG
    AGGGTGAGTG TTCAGTCCCT CGAGTCACGA TTGGGTGCCC TGAGCCCTGC AGAAGACCTG
    GATGGCACTT ATCCCTACCA GAGTGGTCAC CCACAAGTAG ATGAAAATTC AGGACATTTG
    GGAGGTGCCT CAAGGACTGG AATGCTTTTG AGACCCAAGT CCCAGGAGCT GAGGCATTTT
    GAGAATGTGA TCAGCCCAGC GTGTGTGGTC TCTCCTCATG CCACTTACTC CACCAGCATC
    ATCCCAAATG CCACACTCTC TTCCTCTTCA GAGATTATTA TTATTCACAC TGCTCAGAGT
    TCAGGACAGC TGGACAGTAA AATCACCAGC TCCTCTTCAT ACTCAAAGAT AAAATCTAGA
    GACCACCTCC TCTCCAGGCA TACTAGTAAA GATGATCATC AGTCTCCCAG TGGCAACTGG
    ACAGAGGGTC ACCCCAGCAT TCTTTCACAG GCCTTAGATC CCCACACCTC CAGTGCAACC
    ATGCTATTGT CCCTCTGTGA TTCAGTGGTT TCTCTAAATA CTTCAGCAAA TCGGGAGAAT
    GGGTCCCAAG CCATGAGCTA TAACTGTAAA AACAACCTGA GTTCTCCGGC CCACGCCCAG
    GATGTGGATG GCAAGAGTGA GTCCAGTTAT TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG
    CCCTGGGAAT ATAGTGCCCC CGGTAACGGG CAAGCATGCC CACTGAAGGC TCATTTGACA
    ACGCCTGGTT ACTCCACTCC CGGAAGTAAT GTGAGCAGCT GCAGCTTGGA CCAAACATCC
    ACCAAAGATG ATGCCAGATC CCTGTATTCA GAGGAGCATG ATGGCTACTA CACATCTGTG
    CACCCCGATC CGGGACAGGG ATCTGGAAAC CAGTACAATA GTGATGGCTT TGGGAACCCC
    AGGCACAGTG TGGTCAATGT TTTTGATGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCA
    CATTACCATG ACAAGTCCCT TTCTCGAAAC ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT
    CTGCCACCCT CTCGGACTGA CTCCCTGCGC AGGATTCCCA AGAAGGGTGT CCAGTCCAAT
    GGGCAGGCGC TGAACGAGAG CCTGATCGCC TCGCTGCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG AGCCCCTGCA GCGACTTTGA AGAGCCCTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACGGTG AGTGCGGGCA GCAGCATGAC CTCGGCCACC
    ACCCCCAACG TCTACTCCCT GTGCGGGGCC ACGCCGTCGC AGAGTGACAC GAGCAGCGTC
    AAGTCTGAGT ACACAGACCC TTGGGGTTAC TACATTGACT ACACAGGCAT GCAGGAAGAC
    CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC AGCAGTGGGG CGCCGGCTGG AAATGGGCCA
    GTCCACCACG TCCAGGAGGG ATCCAGGACC CAAGTGCCTG GTGGCTCAGT CAAACCAAAG
    ATCACGTCGC CAGAGAAGTC CCACAGAGTC ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG
    TCGAATACAC CCACAGCACT CACCCCTGTG CCTGTGTTTT TAAAATCAGT GTCACCAGCA
    AATGGGAAGG GGAAGACCAA GCCCAAGGTG CCAGAAAGGA AGTCCTCTCT GATATGTTCA
    GTATCCATCT CCTCATCGTC CACGTCTCTG TCTTCCAATA CTTCCACGGA AGGAAGCGGG
    ACAATGAAGA AGCTGGACTC GGTGCTGGCC TCCCCCACTG CGGGTGCTTC TCTCCTTTCT
    CTCCCCTCAC CGTGTGCTGC TGACAAGTCT CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA
    GATTCCCCTG ACGGCTCTCT GCCTCAGTCT CCCCTCTTCC CCCCTCCACC ACCGGAAGTT
    CTCACTCCCT TCTCTGCCCC TGCCAGCGGG TGCCTCCCTC CTTCCCCCAG GGCACTCAGC
    CCCCTTACTC TGGGTGCATC TGCTTCCCTG CCACTGCCCC CCGCTGCCCT GCTCTCCTCA
    GCTCCCCCGC CTGCCCCACC CCTGGACCCT ACATTGATGA AAGATGCCAG GCCTTCTTTC
    AAAAAACCTG GCCAGCCCGA GTGCTCCCGG GAGGGCTTCC GGCAGCCTTG TAACAAGGAG
    GAAGGCGGTA GGCCCCCCAT GCCCCTGATA ACCACTGAAA CGTTGCAGAT GGTACAGTTG
    AGGCCCGTGA AAAAGAACTC AGGAACCGAG CAAGCACTGT TATATGAACA AGTATCTCAG
    GAAAAACTAA CTCCGGTCGT CCCCCAGTAT CATTTAAAGC CATCTGCTTT CTTGAAATCC
    CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG
    CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT CACCGGGACG GTGCAGCCGA GCATGGTCCC
    CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC
    CCGCTCCAGG AGCCCCCCGG CCCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG
    CCCAAACTGT TTCTCGTGGT GCCACCTCCG CAGAGAGATC TCACAGCGGA GCCCGCGGAG
    CCCGCGAGCG GAGCATCACC CGGCCCCAGG CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC
    AGCGCTGGTC CTGATGAGCC GGGCTCTGGC AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA
    GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA GCCGACGTCC CCATGGTGCA GCCCGACGTG
    CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT GCCGGGACCA GTGCCGACGG CGCAGGCGAC
    GTGGAGAGCC GCCTGCCTCT CCAGGACGGA CGGCCTGGGC TGCCAGAGCC TGACGCAGCC
    GGCTCTTCCT CGGAGGCCTG TGACTTCCCC AGGGAAGAAG GGAGTGATGA GGTGATGACC
    CCCAGTAGAC CCCGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGATC CAAGAGGAAA
    GTCCTGGGCC GGAAGGATTC AGATGACGAT CACTCCCGAA ATCATTCTCC CTCCCCTCCG
    GTGACGCCCA CCGGTGCGGC CCCCAGCCTG GCTTCCCCCA AGCAAGTGGG GTCAATTCAG
    AGAAGTGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTAAAAAAG
    GGGAGCCGCT CGGACACCAG CGCCCGCATG TCTGCAGCTG AGATGCTAAA GAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCAGAGCCT TCGCCAGATA CTCCCGAGAG CCCGTCAAGC
    TGCTCCCCCA GCAAGAACAG AAGGGCCCAG GAGGAGTGGG CCAAGAACGA AGGCCTGATG
    CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG TACGGGCGCA GTCGAACTCC ACCTTCTGCA
    GCCAGCAGCA GGTACAGCAT GCGGAACCGA ATCCAGAGCA GTCCCATGAC TGTCATCTCA
    GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA GATAGCCGGG CTCACGGGGC CCTGGGCACC
    GCCAGGGGGT GTTCCATGAA TGGACTAGGT GGGGAGCCCA CCACCCCCCT GCAGGCACAG
    GGTCCTGGCC CTGCTGATGG GATAGTCAGC GCAGAGGGCA CACATGGAAC AGAACAACGT
    GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285859
    Clone ID Related Accession (Same CDS sequence) XM_022417481.1 , XM_022417475.1
    Accession Version XM_022417475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCACCAC CATCAAGCAG GCACAAAGCG GTTTCCAACC TAGATGAGGA GAGTAGATGG 
    ACAGTCCACT ACACTGCTCC ATGGCACCAG CAGGAGAATG TGTTCCTTCC CACCACAAGA
    CCACCCTGTG TAGAGGACCT GCACCGCCAA GCCAAGCTCA ACCTCAAATC AGTATTGAGG
    GAATGCGATA AACTAAGACG AGATGGCTAC CGAAGTTCTC AGTACTACTC CCAGGGGCCC
    ACCTTTGCAG CCAATGCCAG CCCACTCTGT GATGATTATC AAGATGAGGA TGAAGAAACA
    GATCAAAAGT GTTCTGTTTC TTCATCAGAA GAAGAAAGAC TTATTTCCAT CAGGAGACCT
    AAAACGCCAA CCTCAAGTGA CTTCTCTGAC CTTAATACTC AAACAAACTG GACCAAGTCA
    CTTCCTTTGC CAACACCAGA AGAGAAGATG CGACAGCAAG CCCAGACAGT CCAGGCTGAT
    GTGGTTGCCA TTAACATAAC TGGGGAGAAT TTCGATCGCC AGGCCAGTCT TCGGCGGTCT
    CTAATTTACA CAGACACTCT GGTAAGACGA CCGAAGAAAG TCAAAAGGAG AAAGACTATT
    ACAGGAGTCC CTGACAACAT ACAGAAGGAG CTAGCATCAG TCACTGGCCA AGATGATGGT
    GGTGGTCACT CGTTGTACAC CCCAGACCAC TGCTCCACAC TGGGAAGGCT TGATAGCTAT
    TGCTCTGCTG GGCAGCGCTC AGAAACCAGG GACTCCAGCT GTCAGACTGA GGAAGTGAAA
    GTTGTACCCC CTTCAATGAG AAGAATCAGG GCACAGAAGG GGCAAGGCAT TGCTGCCCAG
    ATGAGCCACT TCTCAGGCTC CTCCGGGAAC ATGTCTGTGT TGAGTGATTC TGCAGGTATT
    GTGTTCCCTT CTCGCCTCAA CAATGATGCT GGCTTCCACA GTCTCCCACG TTCTGGAGCG
    AGGGTGAGTG TTCAGTCCCT CGAGTCACGA TTGGGTGCCC TGAGCCCTGC AGAAGACCTG
    GATGGCACTT ATCCCTACCA GAGTGGTCAC CCACAAGTAG ATGAAAATTC AGGACATTTG
    GGAGGTGCCT CAAGGACTGG AATGCTTTTG AGACCCAAGT CCCAGGAGCT GAGGCATTTT
    GAGAATGTGA TCAGCCCAGC GTGTGTGGTC TCTCCTCATG CCACTTACTC CACCAGCATC
    ATCCCAAATG CCACACTCTC TTCCTCTTCA GAGATTATTA TTATTCACAC TGCTCAGAGT
    TCAGGACAGC TGGACAGTAA AATCACCAGC TCCTCTTCAT ACTCAAAGAT AAAATCTAGA
    GACCACCTCC TCTCCAGGCA TACTAGTAAA GATGATCATC AGTCTCCCAG TGGCAACTGG
    ACAGAGGGTC ACCCCAGCAT TCTTTCACAG GCCTTAGATC CCCACACCTC CAGTGCAACC
    ATGCTATTGT CCCTCTGTGA TTCAGTGGTT TCTCTAAATA CTTCAGCAAA TCGGGAGAAT
    GGGTCCCAAG CCATGAGCTA TAACTGTAAA AACAACCTGA GTTCTCCGGC CCACGCCCAG
    GATGTGGATG GCAAGAGTGA GTCCAGTTAT TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG
    CCCTGGGAAT ATAGTGCCCC CGGTAACGGG CAAGCATGCC CACTGAAGGC TCATTTGACA
    ACGCCTGGTT ACTCCACTCC CGGAAGTAAT GTGAGCAGCT GCAGCTTGGA CCAAACATCC
    ACCAAAGATG ATGCCAGATC CCTGTATTCA GAGGAGCATG ATGGCTACTA CACATCTGTG
    CACCCCGATC CGGGACAGGG ATCTGGAAAC CAGTACAATA GTGATGGCTT TGGGAACCCC
    AGGCACAGTG TGGTCAATGT TTTTGATGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCA
    CATTACCATG ACAAGTCCCT TTCTCGAAAC ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT
    CTGCCACCCT CTCGGACTGA CTCCCTGCGC AGGATTCCCA AGAAGGGTGT CCAGTCCAAT
    GGGCAGGCGC TGAACGAGAG CCTGATCGCC TCGCTGCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG AGCCCCTGCA GCGACTTTGA AGAGCCCTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACGGTG AGTGCGGGCA GCAGCATGAC CTCGGCCACC
    ACCCCCAACG TCTACTCCCT GTGCGGGGCC ACGCCGTCGC AGAGTGACAC GAGCAGCGTC
    AAGTCTGAGT ACACAGACCC TTGGGGTTAC TACATTGACT ACACAGGCAT GCAGGAAGAC
    CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC AGCAGTGGGG CGCCGGCTGG AAATGGGCCA
    GTCCACCACG TCCAGGAGGG ATCCAGGACC CAAGTGCCTG GTGGCTCAGT CAAACCAAAG
    ATCACGTCGC CAGAGAAGTC CCACAGAGTC ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG
    TCGAATACAC CCACAGCACT CACCCCTGTG CCTGTGTTTT TAAAATCAGT GTCACCAGCA
    AATGGGAAGG GGAAGACCAA GCCCAAGGTG CCAGAAAGGA AGTCCTCTCT GATATGTTCA
    GTATCCATCT CCTCATCGTC CACGTCTCTG TCTTCCAATA CTTCCACGGA AGGAAGCGGG
    ACAATGAAGA AGCTGGACTC GGTGCTGGCC TCCCCCACTG CGGGTGCTTC TCTCCTTTCT
    CTCCCCTCAC CGTGTGCTGC TGACAAGTCT CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA
    GATTCCCCTG ACGGCTCTCT GCCTCAGTCT CCCCTCTTCC CCCCTCCACC ACCGGAAGTT
    CTCACTCCCT TCTCTGCCCC TGCCAGCGGG TGCCTCCCTC CTTCCCCCAG GGCACTCAGC
    CCCCTTACTC TGGGTGCATC TGCTTCCCTG CCACTGCCCC CCGCTGCCCT GCTCTCCTCA
    GCTCCCCCGC CTGCCCCACC CCTGGACCCT ACATTGATGA AAGATGCCAG GCCTTCTTTC
    AAAAAACCTG GCCAGCCCGA GTGCTCCCGG GAGGGCTTCC GGCAGCCTTG TAACAAGGAG
    GAAGGCGGTA GGCCCCCCAT GCCCCTGATA ACCACTGAAA CGTTGCAGAT GGTACAGTTG
    AGGCCCGTGA AAAAGAACTC AGGAACCGAG CAAGCACTGT TATATGAACA AGTATCTCAG
    GAAAAACTAA CTCCGGTCGT CCCCCAGTAT CATTTAAAGC CATCTGCTTT CTTGAAATCC
    CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG
    CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT CACCGGGACG GTGCAGCCGA GCATGGTCCC
    CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC
    CCGCTCCAGG AGCCCCCCGG CCCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG
    CCCAAACTGT TTCTCGTGGT GCCACCTCCG CAGAGAGATC TCACAGCGGA GCCCGCGGAG
    CCCGCGAGCG GAGCATCACC CGGCCCCAGG CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC
    AGCGCTGGTC CTGATGAGCC GGGCTCTGGC AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA
    GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA GCCGACGTCC CCATGGTGCA GCCCGACGTG
    CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT GCCGGGACCA GTGCCGACGG CGCAGGCGAC
    GTGGAGAGCC GCCTGCCTCT CCAGGACGGA CGGCCTGGGC TGCCAGAGCC TGACGCAGCC
    GGCTCTTCCT CGGAGGCCTG TGACTTCCCC AGGGAAGAAG GGAGTGATGA GGTGATGACC
    CCCAGTAGAC CCCGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGATC CAAGAGGAAA
    GTCCTGGGCC GGAAGGATTC AGATGACGAT CACTCCCGAA ATCATTCTCC CTCCCCTCCG
    GTGACGCCCA CCGGTGCGGC CCCCAGCCTG GCTTCCCCCA AGCAAGTGGG GTCAATTCAG
    AGAAGTGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTAAAAAAG
    GGGAGCCGCT CGGACACCAG CGCCCGCATG TCTGCAGCTG AGATGCTAAA GAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCAGAGCCT TCGCCAGATA CTCCCGAGAG CCCGTCAAGC
    TGCTCCCCCA GCAAGAACAG AAGGGCCCAG GAGGAGTGGG CCAAGAACGA AGGCCTGATG
    CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG TACGGGCGCA GTCGAACTCC ACCTTCTGCA
    GCCAGCAGCA GGTACAGCAT GCGGAACCGA ATCCAGAGCA GTCCCATGAC TGTCATCTCA
    GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA GATAGCCGGG CTCACGGGGC CCTGGGCACC
    GCCAGGGGGT GTTCCATGAA TGGACTAGGT GGGGAGCCCA CCACCCCCCT GCAGGCACAG
    GGTCCTGGCC CTGCTGATGG GATAGTCAGC GCAGAGGGCA CACATGGAAC AGAACAACGT
    GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273183.1
    CDS408..5114
    Translation

    Target ORF information:

    RefSeq Version XM_022417475.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCACCAC CATCAAGCAG GCACAAAGCG GTTTCCAACC TAGATGAGGA GAGTAGATGG 
    ACAGTCCACT ACACTGCTCC ATGGCACCAG CAGGAGAATG TGTTCCTTCC CACCACAAGA
    CCACCCTGTG TAGAGGACCT GCACCGCCAA GCCAAGCTCA ACCTCAAATC AGTATTGAGG
    GAATGCGATA AACTAAGACG AGATGGCTAC CGAAGTTCTC AGTACTACTC CCAGGGGCCC
    ACCTTTGCAG CCAATGCCAG CCCACTCTGT GATGATTATC AAGATGAGGA TGAAGAAACA
    GATCAAAAGT GTTCTGTTTC TTCATCAGAA GAAGAAAGAC TTATTTCCAT CAGGAGACCT
    AAAACGCCAA CCTCAAGTGA CTTCTCTGAC CTTAATACTC AAACAAACTG GACCAAGTCA
    CTTCCTTTGC CAACACCAGA AGAGAAGATG CGACAGCAAG CCCAGACAGT CCAGGCTGAT
    GTGGTTGCCA TTAACATAAC TGGGGAGAAT TTCGATCGCC AGGCCAGTCT TCGGCGGTCT
    CTAATTTACA CAGACACTCT GGTAAGACGA CCGAAGAAAG TCAAAAGGAG AAAGACTATT
    ACAGGAGTCC CTGACAACAT ACAGAAGGAG CTAGCATCAG TCACTGGCCA AGATGATGGT
    GGTGGTCACT CGTTGTACAC CCCAGACCAC TGCTCCACAC TGGGAAGGCT TGATAGCTAT
    TGCTCTGCTG GGCAGCGCTC AGAAACCAGG GACTCCAGCT GTCAGACTGA GGAAGTGAAA
    GTTGTACCCC CTTCAATGAG AAGAATCAGG GCACAGAAGG GGCAAGGCAT TGCTGCCCAG
    ATGAGCCACT TCTCAGGCTC CTCCGGGAAC ATGTCTGTGT TGAGTGATTC TGCAGGTATT
    GTGTTCCCTT CTCGCCTCAA CAATGATGCT GGCTTCCACA GTCTCCCACG TTCTGGAGCG
    AGGGTGAGTG TTCAGTCCCT CGAGTCACGA TTGGGTGCCC TGAGCCCTGC AGAAGACCTG
    GATGGCACTT ATCCCTACCA GAGTGGTCAC CCACAAGTAG ATGAAAATTC AGGACATTTG
    GGAGGTGCCT CAAGGACTGG AATGCTTTTG AGACCCAAGT CCCAGGAGCT GAGGCATTTT
    GAGAATGTGA TCAGCCCAGC GTGTGTGGTC TCTCCTCATG CCACTTACTC CACCAGCATC
    ATCCCAAATG CCACACTCTC TTCCTCTTCA GAGATTATTA TTATTCACAC TGCTCAGAGT
    TCAGGACAGC TGGACAGTAA AATCACCAGC TCCTCTTCAT ACTCAAAGAT AAAATCTAGA
    GACCACCTCC TCTCCAGGCA TACTAGTAAA GATGATCATC AGTCTCCCAG TGGCAACTGG
    ACAGAGGGTC ACCCCAGCAT TCTTTCACAG GCCTTAGATC CCCACACCTC CAGTGCAACC
    ATGCTATTGT CCCTCTGTGA TTCAGTGGTT TCTCTAAATA CTTCAGCAAA TCGGGAGAAT
    GGGTCCCAAG CCATGAGCTA TAACTGTAAA AACAACCTGA GTTCTCCGGC CCACGCCCAG
    GATGTGGATG GCAAGAGTGA GTCCAGTTAT TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG
    CCCTGGGAAT ATAGTGCCCC CGGTAACGGG CAAGCATGCC CACTGAAGGC TCATTTGACA
    ACGCCTGGTT ACTCCACTCC CGGAAGTAAT GTGAGCAGCT GCAGCTTGGA CCAAACATCC
    ACCAAAGATG ATGCCAGATC CCTGTATTCA GAGGAGCATG ATGGCTACTA CACATCTGTG
    CACCCCGATC CGGGACAGGG ATCTGGAAAC CAGTACAATA GTGATGGCTT TGGGAACCCC
    AGGCACAGTG TGGTCAATGT TTTTGATGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCA
    CATTACCATG ACAAGTCCCT TTCTCGAAAC ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT
    CTGCCACCCT CTCGGACTGA CTCCCTGCGC AGGATTCCCA AGAAGGGTGT CCAGTCCAAT
    GGGCAGGCGC TGAACGAGAG CCTGATCGCC TCGCTGCAGC ACTCGCTGCA GCTGAACCTC
    CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG AGCCCCTGCA GCGACTTTGA AGAGCCCTGG
    CTGCCTCGCT CCCGCAGCCA GAGCACGGTG AGTGCGGGCA GCAGCATGAC CTCGGCCACC
    ACCCCCAACG TCTACTCCCT GTGCGGGGCC ACGCCGTCGC AGAGTGACAC GAGCAGCGTC
    AAGTCTGAGT ACACAGACCC TTGGGGTTAC TACATTGACT ACACAGGCAT GCAGGAAGAC
    CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC AGCAGTGGGG CGCCGGCTGG AAATGGGCCA
    GTCCACCACG TCCAGGAGGG ATCCAGGACC CAAGTGCCTG GTGGCTCAGT CAAACCAAAG
    ATCACGTCGC CAGAGAAGTC CCACAGAGTC ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG
    TCGAATACAC CCACAGCACT CACCCCTGTG CCTGTGTTTT TAAAATCAGT GTCACCAGCA
    AATGGGAAGG GGAAGACCAA GCCCAAGGTG CCAGAAAGGA AGTCCTCTCT GATATGTTCA
    GTATCCATCT CCTCATCGTC CACGTCTCTG TCTTCCAATA CTTCCACGGA AGGAAGCGGG
    ACAATGAAGA AGCTGGACTC GGTGCTGGCC TCCCCCACTG CGGGTGCTTC TCTCCTTTCT
    CTCCCCTCAC CGTGTGCTGC TGACAAGTCT CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA
    GATTCCCCTG ACGGCTCTCT GCCTCAGTCT CCCCTCTTCC CCCCTCCACC ACCGGAAGTT
    CTCACTCCCT TCTCTGCCCC TGCCAGCGGG TGCCTCCCTC CTTCCCCCAG GGCACTCAGC
    CCCCTTACTC TGGGTGCATC TGCTTCCCTG CCACTGCCCC CCGCTGCCCT GCTCTCCTCA
    GCTCCCCCGC CTGCCCCACC CCTGGACCCT ACATTGATGA AAGATGCCAG GCCTTCTTTC
    AAAAAACCTG GCCAGCCCGA GTGCTCCCGG GAGGGCTTCC GGCAGCCTTG TAACAAGGAG
    GAAGGCGGTA GGCCCCCCAT GCCCCTGATA ACCACTGAAA CGTTGCAGAT GGTACAGTTG
    AGGCCCGTGA AAAAGAACTC AGGAACCGAG CAAGCACTGT TATATGAACA AGTATCTCAG
    GAAAAACTAA CTCCGGTCGT CCCCCAGTAT CATTTAAAGC CATCTGCTTT CTTGAAATCC
    CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG
    CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT CACCGGGACG GTGCAGCCGA GCATGGTCCC
    CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC
    CCGCTCCAGG AGCCCCCCGG CCCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG
    CCCAAACTGT TTCTCGTGGT GCCACCTCCG CAGAGAGATC TCACAGCGGA GCCCGCGGAG
    CCCGCGAGCG GAGCATCACC CGGCCCCAGG CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC
    AGCGCTGGTC CTGATGAGCC GGGCTCTGGC AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA
    GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA GCCGACGTCC CCATGGTGCA GCCCGACGTG
    CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT GCCGGGACCA GTGCCGACGG CGCAGGCGAC
    GTGGAGAGCC GCCTGCCTCT CCAGGACGGA CGGCCTGGGC TGCCAGAGCC TGACGCAGCC
    GGCTCTTCCT CGGAGGCCTG TGACTTCCCC AGGGAAGAAG GGAGTGATGA GGTGATGACC
    CCCAGTAGAC CCCGGACCAC AGAAGACCTT TTTGCAGCTA TTCACAGATC CAAGAGGAAA
    GTCCTGGGCC GGAAGGATTC AGATGACGAT CACTCCCGAA ATCATTCTCC CTCCCCTCCG
    GTGACGCCCA CCGGTGCGGC CCCCAGCCTG GCTTCCCCCA AGCAAGTGGG GTCAATTCAG
    AGAAGTGTCC GGAAGAGCAG CACCAGCAGT GACAACTTCA AAGCTCTGCT GCTAAAAAAG
    GGGAGCCGCT CGGACACCAG CGCCCGCATG TCTGCAGCTG AGATGCTAAA GAACACAGAC
    CCTAGATTCC AGAGGTCAAG GTCAGAGCCT TCGCCAGATA CTCCCGAGAG CCCGTCAAGC
    TGCTCCCCCA GCAAGAACAG AAGGGCCCAG GAGGAGTGGG CCAAGAACGA AGGCCTGATG
    CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG TACGGGCGCA GTCGAACTCC ACCTTCTGCA
    GCCAGCAGCA GGTACAGCAT GCGGAACCGA ATCCAGAGCA GTCCCATGAC TGTCATCTCA
    GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA GATAGCCGGG CTCACGGGGC CCTGGGCACC
    GCCAGGGGGT GTTCCATGAA TGGACTAGGT GGGGAGCCCA CCACCCCCCT GCAGGCACAG
    GGTCCTGGCC CTGCTGATGG GATAGTCAGC GCAGAGGGCA CACATGGAAC AGAACAACGT
    GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34437
    Clone ID Related Accession (Same CDS sequence) XM_014112351.2 , XM_014112351.1
    Accession Version XM_014112351.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGGCCT TCATTAATGC AAAGATCAAG TCTTTAATTA AACTTTTTAA GAAAAAAACG 
    GTTTCCAACC TAGATGAGGA GAGTAGATGG ACAGTCCACT ACACTGCTCC ATGGCACCAG
    CAGGAGAATG TGTTCCTTCC CACCACAAGA CCACCCTGTG TAGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTATTGAGG GAATGCGATA AACTAAGACG AGATGGCTAC
    CGAAGTTCTC AGTACTACTC CCAGGGGCCC ACCTTTGCAG CCAATGCCAG CCCACTCTGT
    GATGATTATC AAGATGAGGA TGAAGAAACA GATCAAAAGT GTTCTGTTTC TTCATCAGAA
    GAAGAAAGAC TTATTTCCAT CAGGAGACCT AAAACGCCAA CCTCAAGTGA CTTCTCTGAC
    CTTAATACTC AAACAAACTG GACCAAGTCA CTTCCTTTGC CAACACCAGA AGAGAAGATG
    CGACAGCAAG CCCAGACAGT CCAGGCTGAT GTGGTTGCCA TTAACATAAC TGGGGAGAAT
    TTCGATCGCC AGGCCAGTCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA
    CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG
    CTAGCATCAG TCACTGGCCA AGATGATGGT GGTGGTCACT CGTTGTACAC CCCAGACCAC
    TGCTCCACAC TGGGAAGGCT TGATAGCTAT TGCTCTGCTG GGCAGCGCTC AGAAACCAGG
    GACTCCAGCT GTCAGACTGA GGAAGTGAAA GTTGTACCCC CTTCAATGAG AAGAATCAGG
    GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGAGCCACT TCTCAGGCTC CTCCGGGAAC
    ATGTCTGTGT TGAGTGATTC TGCAGGTATT GTGTTCCCTT CTCGCCTCAA CAATGATGCT
    GGCTTCCACA GTCTCCCACG TTCTGGAGCG AGGGTGAGTG TTCAGTCCCT CGAGTCACGA
    TTGGGTGCCC TGAGCCCTGC AGAAGACCTG GATGGCACTT ATCCCTACCA GAGTGGTCAC
    CCACAAGTAG ATGAAAATTC AGGACATTTG GGAGGTGCCT CAAGGACTGG AATGCTTTTG
    AGACCCAAGT CCCAGGAGCT GAGGCATTTT GAGAATGTGA TCAGCCCAGC GTGTGTGGTC
    TCTCCTCATG CCACTTACTC CACCAGCATC ATCCCAAATG CCACACTCTC TTCCTCTTCA
    GAGATTATTA TTATTCACAC TGCTCAGAGT TCAGGACAGC TGGACAGTAA AATCACCAGC
    TCCTCTTCAT ACTCAAAGAT AAAATCTAGA GACCACCTCC TCTCCAGGCA TACTAGTAAA
    GATGATCATC AGTCTCCCAG TGGCAACTGG ACAGAGGGTC ACCCCAGCAT TCTTTCACAG
    GCCTTAGATC CCCACACCTC CAGTGCAACC ATGCTATTGT CCCTCTGTGA TTCAGTGGTT
    TCTCTAAATA CTTCAGCAAA TCGGGAGAAT GGGTCCCAAG CCATGAGCTA TAACTGTAAA
    AACAACCTGA GTTCTCCGGC CCACGCCCAG GATGTGGATG GCAAGAGTGA GTCCAGTTAT
    TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG CCCTGGGAAT ATAGTGCCCC CGGTAACGGG
    CAAGCATGCC CACTGAAGGC TCATTTGACA ACGCCTGGTT ACTCCACTCC CGGAAGTAAT
    GTGAGCAGCT GCAGCTTGGA CCAAACATCC ACCAAAGATG ATGCCAGATC CCTGTATTCA
    GAGGAGCATG ATGGCTACTA CACATCTGTG CACCCCGATC CGGGACAGGG ATCTGGAAAC
    CAGTACAATA GTGATGGCTT TGGGAACCCC AGGCACAGTG TGGTCAATGT TTTTGATGGA
    AGAGCTCAGA AAAACCAAGG GGACCGGTCA CATTACCATG ACAAGTCCCT TTCTCGAAAC
    ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT CTGCCACCCT CTCGGACTGA CTCCCTGCGC
    AGGATTCCCA AGAAGGGTGT CCAGTCCAAT GGGCAGGCGC TGAACGAGAG CCTGATCGCC
    TCGCTGCAGC ACTCGCTGCA GCTGAACCTC CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG
    AGCCCCTGCA GCGACTTTGA AGAGCCCTGG CTGCCTCGCT CCCGCAGCCA GAGCACGGTG
    AGTGCGGGCA GCAGCATGAC CTCGGCCACC ACCCCCAACG TCTACTCCCT GTGCGGGGCC
    ACGCCGTCGC AGAGTGACAC GAGCAGCGTC AAGTCTGAGT ACACAGACCC TTGGGGTTAC
    TACATTGACT ACACAGGCAT GCAGGAAGAC CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC
    AGCAGTGGGG CGCCGGCTGG AAATGGGCCA GTCCACCACG TCCAGGAGGG ATCCAGGACC
    CAAGTGCCTG GTGGCTCAGT CAAACCAAAG ATCACGTCGC CAGAGAAGTC CCACAGAGTC
    ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAGT GTCACCAGCA AATGGGAAGG GGAAGACCAA GCCCAAGGTG
    CCAGAAAGGA AGTCCTCTCT GATATGTTCA GTATCCATCT CCTCATCGTC CACGTCTCTG
    TCTTCCAATA CTTCCACGGA AGGAAGCGGG ACAATGAAGA AGCTGGACTC GGTGCTGGCC
    TCCCCCACTG CGGGTGCTTC TCTCCTTTCT CTCCCCTCAC CGTGTGCTGC TGACAAGTCT
    CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA GATTCCCCTG ACGGCTCTCT GCCTCAGTCT
    CCCCTCTTCC CCCCTCCACC ACCGGAAGTT CTCACTCCCT TCTCTGCCCC TGCCAGCGGG
    TGCCTCCCTC CTTCCCCCAG GGCACTCAGC CCCCTTACTC TGGGTGCATC TGCTTCCCTG
    CCACTGCCCC CCGCTGCCCT GCTCTCCTCA GCTCCCCCGC CTGCCCCACC CCTGGACCCT
    ACATTGATGA AAGATGCCAG GCCTTCTTTC AAAAAACCTG GCCAGCCCGA GTGCTCCCGG
    GAGGGCTTCC GGCAGCCTTG TAACAAGGAG GAAGGCGGTA GGCCCCCCAT GCCCCTGATA
    ACCACTGAAA CGTTGCAGAT GGTACAGTTG AGGCCCGTGA AAAAGAACTC AGGAACCGAG
    CAAGCACTGT TATATGAACA AGTATCTCAG GAAAAACTAA CTCCGGTCGT CCCCCAGTAT
    CATTTAAAGC CATCTGCTTT CTTGAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT
    CACCGGGACG GTGCAGCCGA GCATGGTCCC CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG
    GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC CCGCTCCAGG AGCCCCCCGG CCCTTCACCC
    AGCAGGAAGC CACCCCCCAT TTCCAAGAAG CCCAAACTGT TTCTCGTGGT GCCACCTCCG
    CAGAGAGATC TCACAGCGGA GCCCGCGGAG CCCGCGAGCG GAGCATCACC CGGCCCCAGG
    CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC AGCGCTGGTC CTGATGAGCC GGGCTCTGGC
    AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA
    GCCGACGTCC CCATGGTGCA GCCCGACGTG CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT
    GCCGGGACCA GTGCCGACGG CGCAGGCGAC GTGGAGAGCC GCCTGCCTCT CCAGGACGGA
    CGGCCTGGGC TGCCAGAGCC TGACGCAGCC GGCTCTTCCT CGGAGGCCTG TGACTTCCCC
    AGGGAAGAAG GGAGTGATGA GGTGATGACC CCCAGTAGAC CCCGGACCAC AGAAGACCTT
    TTTGCAGCTA TTCACAGATC CAAGAGGAAA GTCCTGGGCC GGAAGGATTC AGATGACGAT
    CACTCCCGAA ATCATTCTCC CTCCCCTCCG GTGACGCCCA CCGGTGCGGC CCCCAGCCTG
    GCTTCCCCCA AGCAAGTGGG GTCAATTCAG AGAAGTGTCC GGAAGAGCAG CACCAGCAGT
    GACAACTTCA AAGCTCTGCT GCTAAAAAAG GGGAGCCGCT CGGACACCAG CGCCCGCATG
    TCTGCAGCTG AGATGCTAAA GAACACAGAC CCTAGATTCC AGAGGTCAAG GTCAGAGCCT
    TCGCCAGATA CTCCCGAGAG CCCGTCAAGC TGCTCCCCCA GCAAGAACAG AAGGGCCCAG
    GAGGAGTGGG CCAAGAACGA AGGCCTGATG CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG
    TACGGGCGCA GTCGAACTCC ACCTTCTGCA GCCAGCAGCA GGTACAGCAT GCGGAACCGA
    ATCCAGAGCA GTCCCATGAC TGTCATCTCA GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA
    GATAGCCGGG CTCACGGGGC CCTGGGCACC GCCAGGGGGT GTTCCATGAA TGGACTAGGT
    GGGGAGCCCA CCACCCCCCT GCAGGCACAG GGTCCTGGCC CTGCTGATGG GATAGTCAGC
    GCAGAGGGCA CACATGGAAC AGAACAACGT GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967826.1
    CDS536..5272
    Translation

    Target ORF information:

    RefSeq Version XM_014112351.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 1 (NHSL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGGCCT TCATTAATGC AAAGATCAAG TCTTTAATTA AACTTTTTAA GAAAAAAACG 
    GTTTCCAACC TAGATGAGGA GAGTAGATGG ACAGTCCACT ACACTGCTCC ATGGCACCAG
    CAGGAGAATG TGTTCCTTCC CACCACAAGA CCACCCTGTG TAGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTATTGAGG GAATGCGATA AACTAAGACG AGATGGCTAC
    CGAAGTTCTC AGTACTACTC CCAGGGGCCC ACCTTTGCAG CCAATGCCAG CCCACTCTGT
    GATGATTATC AAGATGAGGA TGAAGAAACA GATCAAAAGT GTTCTGTTTC TTCATCAGAA
    GAAGAAAGAC TTATTTCCAT CAGGAGACCT AAAACGCCAA CCTCAAGTGA CTTCTCTGAC
    CTTAATACTC AAACAAACTG GACCAAGTCA CTTCCTTTGC CAACACCAGA AGAGAAGATG
    CGACAGCAAG CCCAGACAGT CCAGGCTGAT GTGGTTGCCA TTAACATAAC TGGGGAGAAT
    TTCGATCGCC AGGCCAGTCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA
    CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG
    CTAGCATCAG TCACTGGCCA AGATGATGGT GGTGGTCACT CGTTGTACAC CCCAGACCAC
    TGCTCCACAC TGGGAAGGCT TGATAGCTAT TGCTCTGCTG GGCAGCGCTC AGAAACCAGG
    GACTCCAGCT GTCAGACTGA GGAAGTGAAA GTTGTACCCC CTTCAATGAG AAGAATCAGG
    GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGAGCCACT TCTCAGGCTC CTCCGGGAAC
    ATGTCTGTGT TGAGTGATTC TGCAGGTATT GTGTTCCCTT CTCGCCTCAA CAATGATGCT
    GGCTTCCACA GTCTCCCACG TTCTGGAGCG AGGGTGAGTG TTCAGTCCCT CGAGTCACGA
    TTGGGTGCCC TGAGCCCTGC AGAAGACCTG GATGGCACTT ATCCCTACCA GAGTGGTCAC
    CCACAAGTAG ATGAAAATTC AGGACATTTG GGAGGTGCCT CAAGGACTGG AATGCTTTTG
    AGACCCAAGT CCCAGGAGCT GAGGCATTTT GAGAATGTGA TCAGCCCAGC GTGTGTGGTC
    TCTCCTCATG CCACTTACTC CACCAGCATC ATCCCAAATG CCACACTCTC TTCCTCTTCA
    GAGATTATTA TTATTCACAC TGCTCAGAGT TCAGGACAGC TGGACAGTAA AATCACCAGC
    TCCTCTTCAT ACTCAAAGAT AAAATCTAGA GACCACCTCC TCTCCAGGCA TACTAGTAAA
    GATGATCATC AGTCTCCCAG TGGCAACTGG ACAGAGGGTC ACCCCAGCAT TCTTTCACAG
    GCCTTAGATC CCCACACCTC CAGTGCAACC ATGCTATTGT CCCTCTGTGA TTCAGTGGTT
    TCTCTAAATA CTTCAGCAAA TCGGGAGAAT GGGTCCCAAG CCATGAGCTA TAACTGTAAA
    AACAACCTGA GTTCTCCGGC CCACGCCCAG GATGTGGATG GCAAGAGTGA GTCCAGTTAT
    TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG CCCTGGGAAT ATAGTGCCCC CGGTAACGGG
    CAAGCATGCC CACTGAAGGC TCATTTGACA ACGCCTGGTT ACTCCACTCC CGGAAGTAAT
    GTGAGCAGCT GCAGCTTGGA CCAAACATCC ACCAAAGATG ATGCCAGATC CCTGTATTCA
    GAGGAGCATG ATGGCTACTA CACATCTGTG CACCCCGATC CGGGACAGGG ATCTGGAAAC
    CAGTACAATA GTGATGGCTT TGGGAACCCC AGGCACAGTG TGGTCAATGT TTTTGATGGA
    AGAGCTCAGA AAAACCAAGG GGACCGGTCA CATTACCATG ACAAGTCCCT TTCTCGAAAC
    ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT CTGCCACCCT CTCGGACTGA CTCCCTGCGC
    AGGATTCCCA AGAAGGGTGT CCAGTCCAAT GGGCAGGCGC TGAACGAGAG CCTGATCGCC
    TCGCTGCAGC ACTCGCTGCA GCTGAACCTC CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG
    AGCCCCTGCA GCGACTTTGA AGAGCCCTGG CTGCCTCGCT CCCGCAGCCA GAGCACGGTG
    AGTGCGGGCA GCAGCATGAC CTCGGCCACC ACCCCCAACG TCTACTCCCT GTGCGGGGCC
    ACGCCGTCGC AGAGTGACAC GAGCAGCGTC AAGTCTGAGT ACACAGACCC TTGGGGTTAC
    TACATTGACT ACACAGGCAT GCAGGAAGAC CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC
    AGCAGTGGGG CGCCGGCTGG AAATGGGCCA GTCCACCACG TCCAGGAGGG ATCCAGGACC
    CAAGTGCCTG GTGGCTCAGT CAAACCAAAG ATCACGTCGC CAGAGAAGTC CCACAGAGTC
    ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAGT GTCACCAGCA AATGGGAAGG GGAAGACCAA GCCCAAGGTG
    CCAGAAAGGA AGTCCTCTCT GATATGTTCA GTATCCATCT CCTCATCGTC CACGTCTCTG
    TCTTCCAATA CTTCCACGGA AGGAAGCGGG ACAATGAAGA AGCTGGACTC GGTGCTGGCC
    TCCCCCACTG CGGGTGCTTC TCTCCTTTCT CTCCCCTCAC CGTGTGCTGC TGACAAGTCT
    CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA GATTCCCCTG ACGGCTCTCT GCCTCAGTCT
    CCCCTCTTCC CCCCTCCACC ACCGGAAGTT CTCACTCCCT TCTCTGCCCC TGCCAGCGGG
    TGCCTCCCTC CTTCCCCCAG GGCACTCAGC CCCCTTACTC TGGGTGCATC TGCTTCCCTG
    CCACTGCCCC CCGCTGCCCT GCTCTCCTCA GCTCCCCCGC CTGCCCCACC CCTGGACCCT
    ACATTGATGA AAGATGCCAG GCCTTCTTTC AAAAAACCTG GCCAGCCCGA GTGCTCCCGG
    GAGGGCTTCC GGCAGCCTTG TAACAAGGAG GAAGGCGGTA GGCCCCCCAT GCCCCTGATA
    ACCACTGAAA CGTTGCAGAT GGTACAGTTG AGGCCCGTGA AAAAGAACTC AGGAACCGAG
    CAAGCACTGT TATATGAACA AGTATCTCAG GAAAAACTAA CTCCGGTCGT CCCCCAGTAT
    CATTTAAAGC CATCTGCTTT CTTGAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT
    CACCGGGACG GTGCAGCCGA GCATGGTCCC CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG
    GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC CCGCTCCAGG AGCCCCCCGG CCCTTCACCC
    AGCAGGAAGC CACCCCCCAT TTCCAAGAAG CCCAAACTGT TTCTCGTGGT GCCACCTCCG
    CAGAGAGATC TCACAGCGGA GCCCGCGGAG CCCGCGAGCG GAGCATCACC CGGCCCCAGG
    CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC AGCGCTGGTC CTGATGAGCC GGGCTCTGGC
    AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA
    GCCGACGTCC CCATGGTGCA GCCCGACGTG CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT
    GCCGGGACCA GTGCCGACGG CGCAGGCGAC GTGGAGAGCC GCCTGCCTCT CCAGGACGGA
    CGGCCTGGGC TGCCAGAGCC TGACGCAGCC GGCTCTTCCT CGGAGGCCTG TGACTTCCCC
    AGGGAAGAAG GGAGTGATGA GGTGATGACC CCCAGTAGAC CCCGGACCAC AGAAGACCTT
    TTTGCAGCTA TTCACAGATC CAAGAGGAAA GTCCTGGGCC GGAAGGATTC AGATGACGAT
    CACTCCCGAA ATCATTCTCC CTCCCCTCCG GTGACGCCCA CCGGTGCGGC CCCCAGCCTG
    GCTTCCCCCA AGCAAGTGGG GTCAATTCAG AGAAGTGTCC GGAAGAGCAG CACCAGCAGT
    GACAACTTCA AAGCTCTGCT GCTAAAAAAG GGGAGCCGCT CGGACACCAG CGCCCGCATG
    TCTGCAGCTG AGATGCTAAA GAACACAGAC CCTAGATTCC AGAGGTCAAG GTCAGAGCCT
    TCGCCAGATA CTCCCGAGAG CCCGTCAAGC TGCTCCCCCA GCAAGAACAG AAGGGCCCAG
    GAGGAGTGGG CCAAGAACGA AGGCCTGATG CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG
    TACGGGCGCA GTCGAACTCC ACCTTCTGCA GCCAGCAGCA GGTACAGCAT GCGGAACCGA
    ATCCAGAGCA GTCCCATGAC TGTCATCTCA GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA
    GATAGCCGGG CTCACGGGGC CCTGGGCACC GCCAGGGGGT GTTCCATGAA TGGACTAGGT
    GGGGAGCCCA CCACCCCCCT GCAGGCACAG GGTCCTGGCC CTGCTGATGG GATAGTCAGC
    GCAGAGGGCA CACATGGAAC AGAACAACGT GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34437
    Clone ID Related Accession (Same CDS sequence) XM_014112351.2 , XM_014112351.1
    Accession Version XM_014112351.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGGCCT TCATTAATGC AAAGATCAAG TCTTTAATTA AACTTTTTAA GAAAAAAACG 
    GTTTCCAACC TAGATGAGGA GAGTAGATGG ACAGTCCACT ACACTGCTCC ATGGCACCAG
    CAGGAGAATG TGTTCCTTCC CACCACAAGA CCACCCTGTG TAGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTATTGAGG GAATGCGATA AACTAAGACG AGATGGCTAC
    CGAAGTTCTC AGTACTACTC CCAGGGGCCC ACCTTTGCAG CCAATGCCAG CCCACTCTGT
    GATGATTATC AAGATGAGGA TGAAGAAACA GATCAAAAGT GTTCTGTTTC TTCATCAGAA
    GAAGAAAGAC TTATTTCCAT CAGGAGACCT AAAACGCCAA CCTCAAGTGA CTTCTCTGAC
    CTTAATACTC AAACAAACTG GACCAAGTCA CTTCCTTTGC CAACACCAGA AGAGAAGATG
    CGACAGCAAG CCCAGACAGT CCAGGCTGAT GTGGTTGCCA TTAACATAAC TGGGGAGAAT
    TTCGATCGCC AGGCCAGTCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA
    CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG
    CTAGCATCAG TCACTGGCCA AGATGATGGT GGTGGTCACT CGTTGTACAC CCCAGACCAC
    TGCTCCACAC TGGGAAGGCT TGATAGCTAT TGCTCTGCTG GGCAGCGCTC AGAAACCAGG
    GACTCCAGCT GTCAGACTGA GGAAGTGAAA GTTGTACCCC CTTCAATGAG AAGAATCAGG
    GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGAGCCACT TCTCAGGCTC CTCCGGGAAC
    ATGTCTGTGT TGAGTGATTC TGCAGGTATT GTGTTCCCTT CTCGCCTCAA CAATGATGCT
    GGCTTCCACA GTCTCCCACG TTCTGGAGCG AGGGTGAGTG TTCAGTCCCT CGAGTCACGA
    TTGGGTGCCC TGAGCCCTGC AGAAGACCTG GATGGCACTT ATCCCTACCA GAGTGGTCAC
    CCACAAGTAG ATGAAAATTC AGGACATTTG GGAGGTGCCT CAAGGACTGG AATGCTTTTG
    AGACCCAAGT CCCAGGAGCT GAGGCATTTT GAGAATGTGA TCAGCCCAGC GTGTGTGGTC
    TCTCCTCATG CCACTTACTC CACCAGCATC ATCCCAAATG CCACACTCTC TTCCTCTTCA
    GAGATTATTA TTATTCACAC TGCTCAGAGT TCAGGACAGC TGGACAGTAA AATCACCAGC
    TCCTCTTCAT ACTCAAAGAT AAAATCTAGA GACCACCTCC TCTCCAGGCA TACTAGTAAA
    GATGATCATC AGTCTCCCAG TGGCAACTGG ACAGAGGGTC ACCCCAGCAT TCTTTCACAG
    GCCTTAGATC CCCACACCTC CAGTGCAACC ATGCTATTGT CCCTCTGTGA TTCAGTGGTT
    TCTCTAAATA CTTCAGCAAA TCGGGAGAAT GGGTCCCAAG CCATGAGCTA TAACTGTAAA
    AACAACCTGA GTTCTCCGGC CCACGCCCAG GATGTGGATG GCAAGAGTGA GTCCAGTTAT
    TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG CCCTGGGAAT ATAGTGCCCC CGGTAACGGG
    CAAGCATGCC CACTGAAGGC TCATTTGACA ACGCCTGGTT ACTCCACTCC CGGAAGTAAT
    GTGAGCAGCT GCAGCTTGGA CCAAACATCC ACCAAAGATG ATGCCAGATC CCTGTATTCA
    GAGGAGCATG ATGGCTACTA CACATCTGTG CACCCCGATC CGGGACAGGG ATCTGGAAAC
    CAGTACAATA GTGATGGCTT TGGGAACCCC AGGCACAGTG TGGTCAATGT TTTTGATGGA
    AGAGCTCAGA AAAACCAAGG GGACCGGTCA CATTACCATG ACAAGTCCCT TTCTCGAAAC
    ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT CTGCCACCCT CTCGGACTGA CTCCCTGCGC
    AGGATTCCCA AGAAGGGTGT CCAGTCCAAT GGGCAGGCGC TGAACGAGAG CCTGATCGCC
    TCGCTGCAGC ACTCGCTGCA GCTGAACCTC CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG
    AGCCCCTGCA GCGACTTTGA AGAGCCCTGG CTGCCTCGCT CCCGCAGCCA GAGCACGGTG
    AGTGCGGGCA GCAGCATGAC CTCGGCCACC ACCCCCAACG TCTACTCCCT GTGCGGGGCC
    ACGCCGTCGC AGAGTGACAC GAGCAGCGTC AAGTCTGAGT ACACAGACCC TTGGGGTTAC
    TACATTGACT ACACAGGCAT GCAGGAAGAC CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC
    AGCAGTGGGG CGCCGGCTGG AAATGGGCCA GTCCACCACG TCCAGGAGGG ATCCAGGACC
    CAAGTGCCTG GTGGCTCAGT CAAACCAAAG ATCACGTCGC CAGAGAAGTC CCACAGAGTC
    ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAGT GTCACCAGCA AATGGGAAGG GGAAGACCAA GCCCAAGGTG
    CCAGAAAGGA AGTCCTCTCT GATATGTTCA GTATCCATCT CCTCATCGTC CACGTCTCTG
    TCTTCCAATA CTTCCACGGA AGGAAGCGGG ACAATGAAGA AGCTGGACTC GGTGCTGGCC
    TCCCCCACTG CGGGTGCTTC TCTCCTTTCT CTCCCCTCAC CGTGTGCTGC TGACAAGTCT
    CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA GATTCCCCTG ACGGCTCTCT GCCTCAGTCT
    CCCCTCTTCC CCCCTCCACC ACCGGAAGTT CTCACTCCCT TCTCTGCCCC TGCCAGCGGG
    TGCCTCCCTC CTTCCCCCAG GGCACTCAGC CCCCTTACTC TGGGTGCATC TGCTTCCCTG
    CCACTGCCCC CCGCTGCCCT GCTCTCCTCA GCTCCCCCGC CTGCCCCACC CCTGGACCCT
    ACATTGATGA AAGATGCCAG GCCTTCTTTC AAAAAACCTG GCCAGCCCGA GTGCTCCCGG
    GAGGGCTTCC GGCAGCCTTG TAACAAGGAG GAAGGCGGTA GGCCCCCCAT GCCCCTGATA
    ACCACTGAAA CGTTGCAGAT GGTACAGTTG AGGCCCGTGA AAAAGAACTC AGGAACCGAG
    CAAGCACTGT TATATGAACA AGTATCTCAG GAAAAACTAA CTCCGGTCGT CCCCCAGTAT
    CATTTAAAGC CATCTGCTTT CTTGAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT
    CACCGGGACG GTGCAGCCGA GCATGGTCCC CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG
    GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC CCGCTCCAGG AGCCCCCCGG CCCTTCACCC
    AGCAGGAAGC CACCCCCCAT TTCCAAGAAG CCCAAACTGT TTCTCGTGGT GCCACCTCCG
    CAGAGAGATC TCACAGCGGA GCCCGCGGAG CCCGCGAGCG GAGCATCACC CGGCCCCAGG
    CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC AGCGCTGGTC CTGATGAGCC GGGCTCTGGC
    AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA
    GCCGACGTCC CCATGGTGCA GCCCGACGTG CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT
    GCCGGGACCA GTGCCGACGG CGCAGGCGAC GTGGAGAGCC GCCTGCCTCT CCAGGACGGA
    CGGCCTGGGC TGCCAGAGCC TGACGCAGCC GGCTCTTCCT CGGAGGCCTG TGACTTCCCC
    AGGGAAGAAG GGAGTGATGA GGTGATGACC CCCAGTAGAC CCCGGACCAC AGAAGACCTT
    TTTGCAGCTA TTCACAGATC CAAGAGGAAA GTCCTGGGCC GGAAGGATTC AGATGACGAT
    CACTCCCGAA ATCATTCTCC CTCCCCTCCG GTGACGCCCA CCGGTGCGGC CCCCAGCCTG
    GCTTCCCCCA AGCAAGTGGG GTCAATTCAG AGAAGTGTCC GGAAGAGCAG CACCAGCAGT
    GACAACTTCA AAGCTCTGCT GCTAAAAAAG GGGAGCCGCT CGGACACCAG CGCCCGCATG
    TCTGCAGCTG AGATGCTAAA GAACACAGAC CCTAGATTCC AGAGGTCAAG GTCAGAGCCT
    TCGCCAGATA CTCCCGAGAG CCCGTCAAGC TGCTCCCCCA GCAAGAACAG AAGGGCCCAG
    GAGGAGTGGG CCAAGAACGA AGGCCTGATG CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG
    TACGGGCGCA GTCGAACTCC ACCTTCTGCA GCCAGCAGCA GGTACAGCAT GCGGAACCGA
    ATCCAGAGCA GTCCCATGAC TGTCATCTCA GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA
    GATAGCCGGG CTCACGGGGC CCTGGGCACC GCCAGGGGGT GTTCCATGAA TGGACTAGGT
    GGGGAGCCCA CCACCCCCCT GCAGGCACAG GGTCCTGGCC CTGCTGATGG GATAGTCAGC
    GCAGAGGGCA CACATGGAAC AGAACAACGT GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967826.1
    CDS12..4748
    Translation

    Target ORF information:

    RefSeq Version XM_014112351.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 1 (NHSL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGGCCT TCATTAATGC AAAGATCAAG TCTTTAATTA AACTTTTTAA GAAAAAAACG 
    GTTTCCAACC TAGATGAGGA GAGTAGATGG ACAGTCCACT ACACTGCTCC ATGGCACCAG
    CAGGAGAATG TGTTCCTTCC CACCACAAGA CCACCCTGTG TAGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTATTGAGG GAATGCGATA AACTAAGACG AGATGGCTAC
    CGAAGTTCTC AGTACTACTC CCAGGGGCCC ACCTTTGCAG CCAATGCCAG CCCACTCTGT
    GATGATTATC AAGATGAGGA TGAAGAAACA GATCAAAAGT GTTCTGTTTC TTCATCAGAA
    GAAGAAAGAC TTATTTCCAT CAGGAGACCT AAAACGCCAA CCTCAAGTGA CTTCTCTGAC
    CTTAATACTC AAACAAACTG GACCAAGTCA CTTCCTTTGC CAACACCAGA AGAGAAGATG
    CGACAGCAAG CCCAGACAGT CCAGGCTGAT GTGGTTGCCA TTAACATAAC TGGGGAGAAT
    TTCGATCGCC AGGCCAGTCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA
    CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG
    CTAGCATCAG TCACTGGCCA AGATGATGGT GGTGGTCACT CGTTGTACAC CCCAGACCAC
    TGCTCCACAC TGGGAAGGCT TGATAGCTAT TGCTCTGCTG GGCAGCGCTC AGAAACCAGG
    GACTCCAGCT GTCAGACTGA GGAAGTGAAA GTTGTACCCC CTTCAATGAG AAGAATCAGG
    GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGAGCCACT TCTCAGGCTC CTCCGGGAAC
    ATGTCTGTGT TGAGTGATTC TGCAGGTATT GTGTTCCCTT CTCGCCTCAA CAATGATGCT
    GGCTTCCACA GTCTCCCACG TTCTGGAGCG AGGGTGAGTG TTCAGTCCCT CGAGTCACGA
    TTGGGTGCCC TGAGCCCTGC AGAAGACCTG GATGGCACTT ATCCCTACCA GAGTGGTCAC
    CCACAAGTAG ATGAAAATTC AGGACATTTG GGAGGTGCCT CAAGGACTGG AATGCTTTTG
    AGACCCAAGT CCCAGGAGCT GAGGCATTTT GAGAATGTGA TCAGCCCAGC GTGTGTGGTC
    TCTCCTCATG CCACTTACTC CACCAGCATC ATCCCAAATG CCACACTCTC TTCCTCTTCA
    GAGATTATTA TTATTCACAC TGCTCAGAGT TCAGGACAGC TGGACAGTAA AATCACCAGC
    TCCTCTTCAT ACTCAAAGAT AAAATCTAGA GACCACCTCC TCTCCAGGCA TACTAGTAAA
    GATGATCATC AGTCTCCCAG TGGCAACTGG ACAGAGGGTC ACCCCAGCAT TCTTTCACAG
    GCCTTAGATC CCCACACCTC CAGTGCAACC ATGCTATTGT CCCTCTGTGA TTCAGTGGTT
    TCTCTAAATA CTTCAGCAAA TCGGGAGAAT GGGTCCCAAG CCATGAGCTA TAACTGTAAA
    AACAACCTGA GTTCTCCGGC CCACGCCCAG GATGTGGATG GCAAGAGTGA GTCCAGTTAT
    TCAGGAGGCA CGGGGCCCAG CAGCTCGGAG CCCTGGGAAT ATAGTGCCCC CGGTAACGGG
    CAAGCATGCC CACTGAAGGC TCATTTGACA ACGCCTGGTT ACTCCACTCC CGGAAGTAAT
    GTGAGCAGCT GCAGCTTGGA CCAAACATCC ACCAAAGATG ATGCCAGATC CCTGTATTCA
    GAGGAGCATG ATGGCTACTA CACATCTGTG CACCCCGATC CGGGACAGGG ATCTGGAAAC
    CAGTACAATA GTGATGGCTT TGGGAACCCC AGGCACAGTG TGGTCAATGT TTTTGATGGA
    AGAGCTCAGA AAAACCAAGG GGACCGGTCA CATTACCATG ACAAGTCCCT TTCTCGAAAC
    ATCTCTTTGA AGAAAGCGAA GAAGCCGCCT CTGCCACCCT CTCGGACTGA CTCCCTGCGC
    AGGATTCCCA AGAAGGGTGT CCAGTCCAAT GGGCAGGCGC TGAACGAGAG CCTGATCGCC
    TCGCTGCAGC ACTCGCTGCA GCTGAACCTC CCGGGCAAGG GCAGCAGCTC GCCCTCCCAG
    AGCCCCTGCA GCGACTTTGA AGAGCCCTGG CTGCCTCGCT CCCGCAGCCA GAGCACGGTG
    AGTGCGGGCA GCAGCATGAC CTCGGCCACC ACCCCCAACG TCTACTCCCT GTGCGGGGCC
    ACGCCGTCGC AGAGTGACAC GAGCAGCGTC AAGTCTGAGT ACACAGACCC TTGGGGTTAC
    TACATTGACT ACACAGGCAT GCAGGAAGAC CCCGGGCCCC CTGGCGGGGG CTGCTCAGCC
    AGCAGTGGGG CGCCGGCTGG AAATGGGCCA GTCCACCACG TCCAGGAGGG ATCCAGGACC
    CAAGTGCCTG GTGGCTCAGT CAAACCAAAG ATCACGTCGC CAGAGAAGTC CCACAGAGTC
    ACTTCTCCAT CCAGTGGGTA TTCCAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAGT GTCACCAGCA AATGGGAAGG GGAAGACCAA GCCCAAGGTG
    CCAGAAAGGA AGTCCTCTCT GATATGTTCA GTATCCATCT CCTCATCGTC CACGTCTCTG
    TCTTCCAATA CTTCCACGGA AGGAAGCGGG ACAATGAAGA AGCTGGACTC GGTGCTGGCC
    TCCCCCACTG CGGGTGCTTC TCTCCTTTCT CTCCCCTCAC CGTGTGCTGC TGACAAGTCT
    CCTTTGCTTC CTCCTCCCCC ACCTTTAGTA GATTCCCCTG ACGGCTCTCT GCCTCAGTCT
    CCCCTCTTCC CCCCTCCACC ACCGGAAGTT CTCACTCCCT TCTCTGCCCC TGCCAGCGGG
    TGCCTCCCTC CTTCCCCCAG GGCACTCAGC CCCCTTACTC TGGGTGCATC TGCTTCCCTG
    CCACTGCCCC CCGCTGCCCT GCTCTCCTCA GCTCCCCCGC CTGCCCCACC CCTGGACCCT
    ACATTGATGA AAGATGCCAG GCCTTCTTTC AAAAAACCTG GCCAGCCCGA GTGCTCCCGG
    GAGGGCTTCC GGCAGCCTTG TAACAAGGAG GAAGGCGGTA GGCCCCCCAT GCCCCTGATA
    ACCACTGAAA CGTTGCAGAT GGTACAGTTG AGGCCCGTGA AAAAGAACTC AGGAACCGAG
    CAAGCACTGT TATATGAACA AGTATCTCAG GAAAAACTAA CTCCGGTCGT CCCCCAGTAT
    CATTTAAAGC CATCTGCTTT CTTGAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGCCTG CCTCTGTGAC AAGCTCGCCG CCGCCTCCTG CCAAGAGCTC GAGTCAGGGT
    CACCGGGACG GTGCAGCCGA GCATGGTCCC CCGAGCCGCA GCCCGGGCGG TGCCAGGGAG
    GCAGAGGCTG GGCCCGGCGC CCGGCCCGCC CCGCTCCAGG AGCCCCCCGG CCCTTCACCC
    AGCAGGAAGC CACCCCCCAT TTCCAAGAAG CCCAAACTGT TTCTCGTGGT GCCACCTCCG
    CAGAGAGATC TCACAGCGGA GCCCGCGGAG CCCGCGAGCG GAGCATCACC CGGCCCCAGG
    CGGGGCGAGG CAGCCGAGAG TGGTAGAGCC AGCGCTGGTC CTGATGAGCC GGGCTCTGGC
    AGCGCCGTGC TCGGGGGAGG AGCCGCAGGA GCCGCGGCCC CGGCCCCGGG TGGAGTGAAA
    GCCGACGTCC CCATGGTGCA GCCCGACGTG CAGCCAGCCC CCAAGCAGGA GGAGCCAGGT
    GCCGGGACCA GTGCCGACGG CGCAGGCGAC GTGGAGAGCC GCCTGCCTCT CCAGGACGGA
    CGGCCTGGGC TGCCAGAGCC TGACGCAGCC GGCTCTTCCT CGGAGGCCTG TGACTTCCCC
    AGGGAAGAAG GGAGTGATGA GGTGATGACC CCCAGTAGAC CCCGGACCAC AGAAGACCTT
    TTTGCAGCTA TTCACAGATC CAAGAGGAAA GTCCTGGGCC GGAAGGATTC AGATGACGAT
    CACTCCCGAA ATCATTCTCC CTCCCCTCCG GTGACGCCCA CCGGTGCGGC CCCCAGCCTG
    GCTTCCCCCA AGCAAGTGGG GTCAATTCAG AGAAGTGTCC GGAAGAGCAG CACCAGCAGT
    GACAACTTCA AAGCTCTGCT GCTAAAAAAG GGGAGCCGCT CGGACACCAG CGCCCGCATG
    TCTGCAGCTG AGATGCTAAA GAACACAGAC CCTAGATTCC AGAGGTCAAG GTCAGAGCCT
    TCGCCAGATA CTCCCGAGAG CCCGTCAAGC TGCTCCCCCA GCAAGAACAG AAGGGCCCAG
    GAGGAGTGGG CCAAGAACGA AGGCCTGATG CCTCGGAGTC TGTCCTTCTC TGGCCCCCGG
    TACGGGCGCA GTCGAACTCC ACCTTCTGCA GCCAGCAGCA GGTACAGCAT GCGGAACCGA
    ATCCAGAGCA GTCCCATGAC TGTCATCTCA GAAGGCGAAG GGGAGGCCAT GGAGCCTGTA
    GATAGCCGGG CTCACGGGGC CCTGGGCACC GCCAGGGGGT GTTCCATGAA TGGACTAGGT
    GGGGAGCCCA CCACCCCCCT GCAGGCACAG GGTCCTGGCC CTGCTGATGG GATAGTCAGC
    GCAGAGGGCA CACATGGAAC AGAACAACGT GGAGGTCCTC AGAGGGAAGA GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.