DGKA cDNA ORF clone, Canis lupus familiaris(dog)

The following DGKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe13001 XM_022424030.1
Latest version!
Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe13001 XM_005625475.2
Latest version!
Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe13001 XM_005625477.3
Latest version!
Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe13001 XM_003639319.4
Latest version!
Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe12999 XM_005625475.3
Latest version!
Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe38762 XM_014117136.1
Latest version!
Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe38763 XM_014117137.2
Latest version!
Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe13001 XM_005625477.2 Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe13001 XM_003639319.3 Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe38763 XM_014117137.1 Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe13001
    Clone ID Related Accession (Same CDS sequence) XM_005625477.2 , XM_003639319.4 , XM_022424030.1 , XM_005625475.2 , XM_005625477.3 , XM_003639319.3
    Accession Version XM_005625477.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625534.1
    CDS328..2532
    Translation

    Target ORF information:

    RefSeq Version XM_005625477.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625477.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13001
    Clone ID Related Accession (Same CDS sequence) XM_005625477.2 , XM_003639319.4 , XM_022424030.1 , XM_005625475.2 , XM_005625477.3 , XM_003639319.3
    Accession Version XM_003639319.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003639319.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003639367.1
    CDS154..2358
    Translation

    Target ORF information:

    RefSeq Version XM_003639319.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003639319.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13001
    Clone ID Related Accession (Same CDS sequence) XM_005625477.2 , XM_003639319.4 , XM_022424030.1 , XM_005625475.2 , XM_005625477.3 , XM_003639319.3
    Accession Version XM_022424030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279738.1
    CDS360..2564
    Translation

    Target ORF information:

    RefSeq Version XM_022424030.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13001
    Clone ID Related Accession (Same CDS sequence) XM_005625477.2 , XM_003639319.4 , XM_022424030.1 , XM_005625475.2 , XM_005625477.3 , XM_003639319.3
    Accession Version XM_005625475.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625532.2
    CDS101..2305
    Translation

    Target ORF information:

    RefSeq Version XM_005625475.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625475.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13001
    Clone ID Related Accession (Same CDS sequence) XM_005625477.2 , XM_003639319.4 , XM_022424030.1 , XM_005625475.2 , XM_005625477.3 , XM_003639319.3
    Accession Version XM_005625477.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625534.1
    CDS336..2540
    Translation

    Target ORF information:

    RefSeq Version XM_005625477.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625477.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13001
    Clone ID Related Accession (Same CDS sequence) XM_005625477.2 , XM_003639319.4 , XM_022424030.1 , XM_005625475.2 , XM_005625477.3 , XM_003639319.3
    Accession Version XM_003639319.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003639319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003639367.1
    CDS675..2879
    Translation

    Target ORF information:

    RefSeq Version XM_003639319.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003639319.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAACG ACAGGGGCCT GATAAGCCCC AGTGATTTCG CCCAGCTGCA GAAGTACATG 
    GAATACTCCA CCAGAAAGGT CAGCGATGTC CTGAAGCTCT TCGAGGAGGG CGAAATGTCA
    GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAC
    CTGGAAGCAG ATAACATCCC CAATCACCTA AGCCTGGCAT TGTTTCAATC CTTCAAGACG
    GGTCACTGCT TAGAAGACAC TGTGATACGT GATGTGGTGT GTCTCAGTGA CGTCTCCTGC
    TACTTTTCCC TTTTGGAGGG TGGCCGCCCA GAAGACAAGT TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT TATCATACAG
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12999
    Clone ID Related Accession (Same CDS sequence) XM_005625475.3
    Accession Version XM_005625475.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625475.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGATCGT GGCCGGGGAG GCGCGCCGGC GACACCGGCG CCCCTTGGAA GCCTAAGCGC 
    AGGCGCGACA AGTACTGCCG AGCTGCCTTT GTGGTCCTCC TGAGGAAAGG GAGAAAGATG
    GCCAACGACA GGGGCCTGAT AAGCCCCAGT GATTTCGCCC AGCTGCAGAA GTACATGGAA
    TACTCCACCA GAAAGGTCAG CGATGTCCTG AAGCTCTTCG AGGAGGGCGA AATGTCAGAA
    TATCTCCAAG GAGATGCCAT TGGGTACGAG GGATTCCAGC AATTTTTGAA AATCTACCTG
    GAAGCAGATA ACATCCCCAA TCACCTAAGC CTGGCATTGT TTCAATCCTT CAAGACGGGT
    CACTGCTTAG AAGACACTGT GATACGTGAT GTGGTGTGTC TCAGTGACGT CTCCTGCTAC
    TTTTCCCTTT TGGAGGGTGG CCGCCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAGAATTAT CATACAGATG
    ATGCGAGTGG CTGAGTACCT GGATTGGGAT GTGTCTGAAC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AAATTGACTA TGACGGCAGC GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    AATGGGCAGC ACATGTGGAG ACCCAAGAGG TTCCCCCGAC CGGTCTACTG CAACCTGTGC
    GAGTCGAGCA TTGGGCTTGG CAAACAGGGG CTGAGCTGCA ACCTCTGTAA GTACGTCGTT
    CATGACCAGT GTGCCATGAA GGCTTTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTATCCAGTC ACATGTGTGG GTGAGAGGAG GCTGTGTGTC TGGCCGTTGT
    GACCGGTGTC AGAAGAAGAT CCGCATTTAC CACAGTCTAG TTGGACTGCA TTGTGTGTGG
    TGCCACCTAG AGATTCATGA TGACTGCCTG CAAGCCATGG GTCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATTTATC CCAGTGTCCT GGCCTCTGGA
    CAGGAGCGTA AAATTAGCAA AACAAGCCAG AAGACCTTGG ATGACTTAAA TTTGAGCACC
    TTTGAGGCTC TGCGGATTGA TCCTGTACCT AATACCCACC CACTTCTGGT CTTCGTCAAC
    CCTAAGAGTG GCGGGAAGCA GGGGGAGAGG GTGCTTTGGA AATTCCAGTA TCTACTAAAT
    CCTCGACAGG TATTCAACCT CCTAAAGGAT GGTCCTGAGC CAGGGCTCAG ATTCTTTAGG
    GATGTTCCTG GTTGCCGGAT TTTGGTGTGT GGTGGAGATG GCACAGTAGG CTGGATTCTA
    GAGACCATAG ACAAAGCCAA CTTGCCTGTT GTGCCTCCCG TTGCTGTGTT GCCCCTGGGC
    ACTGGAAATG ATCTGGCTCG TTGCCTGAGA TGGGGAGGAG GATATGAGGG ACAGAATTTG
    GGGAAGATCC TCAAGGATTT AGAGATGAGT AAGGTGGTAC ATATGGATCG ATGGTCCGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAG AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTTT CCATCGGTGT GGATGCCTCT ATTGCTCACC GATTCCATAT CATGCGAGAG
    AAATATCCTG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TCTGGTACTT CGAATTCGCC
    ACTTCTGAAT CTATCTTCTC AACATGCAAA AAACTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CACTGGATCT GAGCAACCTG TCCCTAGAAG GCATTGCGGT GCTGAACATC
    CCTAGCATGC ATGGTGGTTC CAACCTCTGG GGTGATACCA AGAAACCTCA TGGAGATATC
    CATGGGATCA ACCAGGCCTT AGGCCCTGCA GCTAAAGTCA TCACTGACCC AGACATCCTG
    AAAACCTGTG TACCAGACCT GACTGACAAA CGGCTGGAAG TAGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAGATATA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAAATCA CCTTCCACAC CACAAAAACC CTCCCCATGC AAATTGATGG AGAACCCTGG
    ATGCAGTCGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTTGTGGGC
    CCACCCCCTC GCTCCTCCAA TTTCTTTGGC TTCTTATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625532.3
    CDS16..2337
    Translation

    Target ORF information:

    RefSeq Version XM_005625475.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625475.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGATCGT GGCCGGGGAG GCGCGCCGGC GACACCGGCG CCCCTTGGAA GCCTAAGCGC 
    AGGCGCGACA AGTACTGCCG AGCTGCCTTT GTGGTCCTCC TGAGGAAAGG GAGAAAGATG
    GCCAACGACA GGGGCCTGAT AAGCCCCAGT GATTTCGCCC AGCTGCAGAA GTACATGGAA
    TACTCCACCA GAAAGGTCAG CGATGTCCTG AAGCTCTTCG AGGAGGGCGA AATGTCAGAA
    TATCTCCAAG GAGATGCCAT TGGGTACGAG GGATTCCAGC AATTTTTGAA AATCTACCTG
    GAAGCAGATA ACATCCCCAA TCACCTAAGC CTGGCATTGT TTCAATCCTT CAAGACGGGT
    CACTGCTTAG AAGACACTGT GATACGTGAT GTGGTGTGTC TCAGTGACGT CTCCTGCTAC
    TTTTCCCTTT TGGAGGGTGG CCGCCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAGAATTAT CATACAGATG
    ATGCGAGTGG CTGAGTACCT GGATTGGGAT GTGTCTGAAC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AAATTGACTA TGACGGCAGC GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    AATGGGCAGC ACATGTGGAG ACCCAAGAGG TTCCCCCGAC CGGTCTACTG CAACCTGTGC
    GAGTCGAGCA TTGGGCTTGG CAAACAGGGG CTGAGCTGCA ACCTCTGTAA GTACGTCGTT
    CATGACCAGT GTGCCATGAA GGCTTTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTATCCAGTC ACATGTGTGG GTGAGAGGAG GCTGTGTGTC TGGCCGTTGT
    GACCGGTGTC AGAAGAAGAT CCGCATTTAC CACAGTCTAG TTGGACTGCA TTGTGTGTGG
    TGCCACCTAG AGATTCATGA TGACTGCCTG CAAGCCATGG GTCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATTTATC CCAGTGTCCT GGCCTCTGGA
    CAGGAGCGTA AAATTAGCAA AACAAGCCAG AAGACCTTGG ATGACTTAAA TTTGAGCACC
    TTTGAGGCTC TGCGGATTGA TCCTGTACCT AATACCCACC CACTTCTGGT CTTCGTCAAC
    CCTAAGAGTG GCGGGAAGCA GGGGGAGAGG GTGCTTTGGA AATTCCAGTA TCTACTAAAT
    CCTCGACAGG TATTCAACCT CCTAAAGGAT GGTCCTGAGC CAGGGCTCAG ATTCTTTAGG
    GATGTTCCTG GTTGCCGGAT TTTGGTGTGT GGTGGAGATG GCACAGTAGG CTGGATTCTA
    GAGACCATAG ACAAAGCCAA CTTGCCTGTT GTGCCTCCCG TTGCTGTGTT GCCCCTGGGC
    ACTGGAAATG ATCTGGCTCG TTGCCTGAGA TGGGGAGGAG GATATGAGGG ACAGAATTTG
    GGGAAGATCC TCAAGGATTT AGAGATGAGT AAGGTGGTAC ATATGGATCG ATGGTCCGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAG AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTTT CCATCGGTGT GGATGCCTCT ATTGCTCACC GATTCCATAT CATGCGAGAG
    AAATATCCTG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TCTGGTACTT CGAATTCGCC
    ACTTCTGAAT CTATCTTCTC AACATGCAAA AAACTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CACTGGATCT GAGCAACCTG TCCCTAGAAG GCATTGCGGT GCTGAACATC
    CCTAGCATGC ATGGTGGTTC CAACCTCTGG GGTGATACCA AGAAACCTCA TGGAGATATC
    CATGGGATCA ACCAGGCCTT AGGCCCTGCA GCTAAAGTCA TCACTGACCC AGACATCCTG
    AAAACCTGTG TACCAGACCT GACTGACAAA CGGCTGGAAG TAGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAGATATA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAAATCA CCTTCCACAC CACAAAAACC CTCCCCATGC AAATTGATGG AGAACCCTGG
    ATGCAGTCGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTTGTGGGC
    CCACCCCCTC GCTCCTCCAA TTTCTTTGGC TTCTTATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38762
    Clone ID Related Accession (Same CDS sequence) XM_014117136.1
    Accession Version XM_014117136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase alpha isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATTCCCT CTCCGGGGGC CCTGCCTAGT AGCAGGGATG AGCAAGAAGG AATAGATCCT 
    CCCTCAGTCC TGGGCAAGAG AAGCAGGACG CAGGCCCTGG TGTGGCCAAG GGATTCAGCA
    GTCCAGGGCG CCCCTTGGAA GCCTAAGCGC AGGCGCGACA AGTACTGCCG AGCTGCCTTT
    GTGGTCCTCC TGAGGAAAGG GAGAAAGATG GCCAACGACA GGGGCCTGAT AAGCCCCAGT
    GATTTCGCCC AGCTGCAGAA GTACATGGAA TACTCCACCA GAAAGGTCAG CGATGTCCTG
    AAGCTCTTCG AGGAGGGCGA AATGTCAGAA TATCTCCAAG GAGATGCCAT TGGGTACGAG
    GGATTCCAGC AATTTTTGAA AATCTACCTG GAAGCAGATA ACATCCCCAA TCACCTAAGC
    CTGGCATTGT TTCAATCCTT CAAGACGGGT CACTGCTTAG AAGACACTGT GATACGTGAT
    GTGGTGTGTC TCAGTGACGT CTCCTGCTAC TTTTCCCTTT TGGAGGGTGG CCGCCCAGAA
    GACAAGTTAG AATTCACCTT CAAGCTGTAC GACACGGACA GAAATGGGAT CCTGGACAGC
    TCAGAAGTGG ACAGAATTAT CATACAGATG ATGCGAGTGG CTGAGTACCT GGATTGGGAT
    GTGTCTGAAC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AAATTGACTA TGACGGCAGC
    GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC AATGGGCAGC ACATGTGGAG ACCCAAGAGG
    TTCCCCCGAC CGGTCTACTG CAACCTGTGC GAGTCGAGCA TTGGGCTTGG CAAACAGGGG
    CTGAGCTGCA ACCTCTGTAA GTACGTCGTT CATGACCAGT GTGCCATGAA GGCTTTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTATCCAGTC ACATGTGTGG
    GTGAGAGGAG GCTGTGTGTC TGGCCGTTGT GACCGGTGTC AGAAGAAGAT CCGCATTTAC
    CACAGTCTAG TTGGACTGCA TTGTGTGTGG TGCCACCTAG AGATTCATGA TGACTGCCTG
    CAAGCCATGG GTCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATTTATC CCAGTGTCCT GGCCTCTGGA CAGGAGCGTA AAATTAGCAA AACAAGCCAG
    AAGACCTTGG ATGACTTAAA TTTGAGCACC TTTGAGGCTC TGCGGATTGA TCCTGTACCT
    AATACCCACC CACTTCTGGT CTTCGTCAAC CCTAAGAGTG GCGGGAAGCA GGGGGAGAGG
    GTGCTTTGGA AATTCCAGTA TCTACTAAAT CCTCGACAGG TATTCAACCT CCTAAAGGAT
    GGTCCTGAGC CAGGGCTCAG ATTCTTTAGG GATGTTCCTG GTTGCCGGAT TTTGGTGTGT
    GGTGGAGATG GCACAGTAGG CTGGATTCTA GAGACCATAG ACAAAGCCAA CTTGCCTGTT
    GTGCCTCCCG TTGCTGTGTT GCCCCTGGGC ACTGGAAATG ATCTGGCTCG TTGCCTGAGA
    TGGGGAGGAG GATATGAGGG ACAGAATTTG GGGAAGATCC TCAAGGATTT AGAGATGAGT
    AAGGTGGTAC ATATGGATCG ATGGTCCGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAG
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTTT CCATCGGTGT GGATGCCTCT
    ATTGCTCACC GATTCCATAT CATGCGAGAG AAATATCCTG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TCTGGTACTT CGAATTCGCC ACTTCTGAAT CTATCTTCTC AACATGCAAA
    AAACTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CACTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCATCTG CTGGATGCAG GGGTTGCATC CACAATTATG CCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972611.1
    CDS399..2435
    Translation

    Target ORF information:

    RefSeq Version XM_014117136.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATTCCCT CTCCGGGGGC CCTGCCTAGT AGCAGGGATG AGCAAGAAGG AATAGATCCT 
    CCCTCAGTCC TGGGCAAGAG AAGCAGGACG CAGGCCCTGG TGTGGCCAAG GGATTCAGCA
    GTCCAGGGCG CCCCTTGGAA GCCTAAGCGC AGGCGCGACA AGTACTGCCG AGCTGCCTTT
    GTGGTCCTCC TGAGGAAAGG GAGAAAGATG GCCAACGACA GGGGCCTGAT AAGCCCCAGT
    GATTTCGCCC AGCTGCAGAA GTACATGGAA TACTCCACCA GAAAGGTCAG CGATGTCCTG
    AAGCTCTTCG AGGAGGGCGA AATGTCAGAA TATCTCCAAG GAGATGCCAT TGGGTACGAG
    GGATTCCAGC AATTTTTGAA AATCTACCTG GAAGCAGATA ACATCCCCAA TCACCTAAGC
    CTGGCATTGT TTCAATCCTT CAAGACGGGT CACTGCTTAG AAGACACTGT GATACGTGAT
    GTGGTGTGTC TCAGTGACGT CTCCTGCTAC TTTTCCCTTT TGGAGGGTGG CCGCCCAGAA
    GACAAGTTAG AATTCACCTT CAAGCTGTAC GACACGGACA GAAATGGGAT CCTGGACAGC
    TCAGAAGTGG ACAGAATTAT CATACAGATG ATGCGAGTGG CTGAGTACCT GGATTGGGAT
    GTGTCTGAAC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AAATTGACTA TGACGGCAGC
    GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC AATGGGCAGC ACATGTGGAG ACCCAAGAGG
    TTCCCCCGAC CGGTCTACTG CAACCTGTGC GAGTCGAGCA TTGGGCTTGG CAAACAGGGG
    CTGAGCTGCA ACCTCTGTAA GTACGTCGTT CATGACCAGT GTGCCATGAA GGCTTTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTATCCAGTC ACATGTGTGG
    GTGAGAGGAG GCTGTGTGTC TGGCCGTTGT GACCGGTGTC AGAAGAAGAT CCGCATTTAC
    CACAGTCTAG TTGGACTGCA TTGTGTGTGG TGCCACCTAG AGATTCATGA TGACTGCCTG
    CAAGCCATGG GTCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATTTATC CCAGTGTCCT GGCCTCTGGA CAGGAGCGTA AAATTAGCAA AACAAGCCAG
    AAGACCTTGG ATGACTTAAA TTTGAGCACC TTTGAGGCTC TGCGGATTGA TCCTGTACCT
    AATACCCACC CACTTCTGGT CTTCGTCAAC CCTAAGAGTG GCGGGAAGCA GGGGGAGAGG
    GTGCTTTGGA AATTCCAGTA TCTACTAAAT CCTCGACAGG TATTCAACCT CCTAAAGGAT
    GGTCCTGAGC CAGGGCTCAG ATTCTTTAGG GATGTTCCTG GTTGCCGGAT TTTGGTGTGT
    GGTGGAGATG GCACAGTAGG CTGGATTCTA GAGACCATAG ACAAAGCCAA CTTGCCTGTT
    GTGCCTCCCG TTGCTGTGTT GCCCCTGGGC ACTGGAAATG ATCTGGCTCG TTGCCTGAGA
    TGGGGAGGAG GATATGAGGG ACAGAATTTG GGGAAGATCC TCAAGGATTT AGAGATGAGT
    AAGGTGGTAC ATATGGATCG ATGGTCCGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAG
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTTT CCATCGGTGT GGATGCCTCT
    ATTGCTCACC GATTCCATAT CATGCGAGAG AAATATCCTG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TCTGGTACTT CGAATTCGCC ACTTCTGAAT CTATCTTCTC AACATGCAAA
    AAACTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CACTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCATCTG CTGGATGCAG GGGTTGCATC CACAATTATG CCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38763
    Clone ID Related Accession (Same CDS sequence) XM_014117137.1 , XM_014117137.2
    Accession Version XM_014117137.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase alpha isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972612.1
    CDS81..1865
    Translation

    Target ORF information:

    RefSeq Version XM_014117137.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38763
    Clone ID Related Accession (Same CDS sequence) XM_014117137.1 , XM_014117137.2
    Accession Version XM_014117137.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972612.1
    CDS91..1875
    Translation

    Target ORF information:

    RefSeq Version XM_014117137.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117137.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAGTA CCTGGATTGG GATGTGTCTG AACTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAAATTGA CTATGACGGC AGCGGCTCTG TCTCCCTCGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGGCT TGGCAAACAG GGGCTGAGCT GCAACCTCTG TAAGTACGTC
    GTTCATGACC AGTGTGCCAT GAAGGCTTTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTATCCA GTCACATGTG TGGGTGAGAG GAGGCTGTGT GTCTGGCCGT
    TGTGACCGGT GTCAGAAGAA GATCCGCATT TACCACAGTC TAGTTGGACT GCATTGTGTG
    TGGTGCCACC TAGAGATTCA TGATGACTGC CTGCAAGCCA TGGGTCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATTT ATCCCAGTGT CCTGGCCTCT
    GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCT TGGATGACTT AAATTTGAGC
    ACCTTTGAGG CTCTGCGGAT TGATCCTGTA CCTAATACCC ACCCACTTCT GGTCTTCGTC
    AACCCTAAGA GTGGCGGGAA GCAGGGGGAG AGGGTGCTTT GGAAATTCCA GTATCTACTA
    AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTT
    AGGGATGTTC CTGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CCGTTGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTG AGATGGGGAG GAGGATATGA GGGACAGAAT
    TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TTTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA TATCATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTCTGGTA CTTCGAATTC
    GCCACTTCTG AATCTATCTT CTCAACATGC AAAAAACTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC GGTGCTGAAC
    ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAAACC TCATGGAGAT
    ATCCATGGGA TCAACCAGGC CTTAGGCCCT GCAGCTAAAG TCATCACTGA CCCAGACATC
    CTGAAAACCT GTGTACCAGA CCTGACTGAC AAACGGCTGG AAGTAGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAGAT ATATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA TGGAGAACCC
    TGGATGCAGT CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG
    GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.