PPEF1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPEF1
Entrez Gene ID	100688966
Full Name	protein phosphatase with EF-hand domain 1
General protein information	Preferred Names protein phosphatase with EF-hand domain 1 Names serine/threonine-protein phosphatase with EF-hands 1 protein phosphatase, EF-hand calcium binding domain 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014111939.2	XP_013967414.1	serine/threonine-protein phosphatase with EF-hands 1
XM_014111940.1	XP_013967415.1	serine/threonine-protein phosphatase with EF-hands 1
XM_014111941.1	XP_013967416.1	serine/threonine-protein phosphatase with EF-hands 1
XM_014111940.2	XP_013967415.1	serine/threonine-protein phosphatase with EF-hands 1
XM_022415941.1	XP_022271649.1	serine/threonine-protein phosphatase with EF-hands 1
XM_014111939.1	XP_013967414.1	serine/threonine-protein phosphatase with EF-hands 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPEF1	XP_003953993.1
Caenorhabditis elegans (roundworm)	pef-1	NP_001254836.1
	PPEF1	XP_001083653.1
Bos taurus (cattle)	PPEF1	XP_005228463.1
Gallus gallus (chicken)	PPEF1	XP_003640538.2
Canis lupus familiaris (dog)	PPEF1	XP_003640299.2
Homo sapiens (human)	PPEF1	NP_006231.2
Danio rerio (zebrafish)	ppef1	NP_001108202.1
Mus musculus (house mouse)	Ppef1	NP_035277.1
Rattus norvegicus (Norway rat)	Ppef1	NP_001030107.1
Xenopus tropicalis (tropical clawed frog)	ppef1	XP_002936093.2

Gene Ontology

The following PPEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe45761	XM_014111940.2 Latest version!	Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe45761	XM_014111939.2 Latest version!	Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe45761	XM_014111941.1 Latest version!	Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe45761	XM_022415941.1 Latest version!	Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe45761	XM_014111940.1	Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe45761	XM_014111939.1	Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPEF1 ( XM_014111939.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967414.1 Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe45761
Clone ID Related Accession (Same CDS sequence)	XM_014111939.2 , XM_014111940.1 , XM_014111941.1 , XM_014111940.2 , XM_022415941.1 , XM_014111939.1
Accession Version	XM_014111939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967414.1
CDS	512..2497
Translation	MSTHDLEDMGCGTSSSTKDRKSEKVLKAALVIQNWYRGYSARLKARQRYALTIFQSIEYADEQGQLQLSNFFSFMLENYTQIHKKDPELLARLFGSTLQDYKDRQDYVSHIDVPDSYHGPRLQFPLTFTDIDLLVEAFKQQQILHAHYVLEVLFETKKVLKQMPNFTHVKTSPSKELTICGDLHGKLDDLFLIFYKNGLPSGSNTYIFNGDFVDRGKHSIEILMILFVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKIHGKKILQILEDVYTWLPIGTIIDSEILVIHGGISESTDLNLLHRIERNKMKSVLMSPIPLNEDHNNDLKKNKINTTIIPREVKTNGSLSEQLTKHEWEQVIDILWSDPRGKRGCYPNTSRGGGCYFGPDITSKVLNKYQLKMLIRSHECKPEGYEICHDGKVVTVFSASNYYEEGSNRGAYIKLCSGMNLRFFQYQVTKATCLRPLYQRVNTIESSAIRILKERMISRKTDLIHAFQLQDRSKSGKLPMGQWAFSMENVLGLNLPWRSLSPHLVTTDKSGNINYMSSFEDIHIQKPVKEAQSTLIETLYRYRSDLQIIFNIIDSDHSGLISMEEFRAMWKLFNSHYSIHIDDFQIDDLAERMDLNKDGSIDFNEFLKAFYVVHKLDNVNKSDSKLA

Target ORF information:

RefSeq Version	XM_014111939.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111939.2

ORF Insert Sequence:

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_014111940.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967415.1 Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45761
Clone ID Related Accession (Same CDS sequence)	XM_014111939.2 , XM_014111940.1 , XM_014111941.1 , XM_014111940.2 , XM_022415941.1 , XM_014111939.1
Accession Version	XM_014111940.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967415.1
CDS	263..2248
Translation	MSTHDLEDMGCGTSSSTKDRKSEKVLKAALVIQNWYRGYSARLKARQRYALTIFQSIEYADEQGQLQLSNFFSFMLENYTQIHKKDPELLARLFGSTLQDYKDRQDYVSHIDVPDSYHGPRLQFPLTFTDIDLLVEAFKQQQILHAHYVLEVLFETKKVLKQMPNFTHVKTSPSKELTICGDLHGKLDDLFLIFYKNGLPSGSNTYIFNGDFVDRGKHSIEILMILFVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKIHGKKILQILEDVYTWLPIGTIIDSEILVIHGGISESTDLNLLHRIERNKMKSVLMSPIPLNEDHNNDLKKNKINTTIIPREVKTNGSLSEQLTKHEWEQVIDILWSDPRGKRGCYPNTSRGGGCYFGPDITSKVLNKYQLKMLIRSHECKPEGYEICHDGKVVTVFSASNYYEEGSNRGAYIKLCSGMNLRFFQYQVTKATCLRPLYQRVNTIESSAIRILKERMISRKTDLIHAFQLQDRSKSGKLPMGQWAFSMENVLGLNLPWRSLSPHLVTTDKSGNINYMSSFEDIHIQKPVKEAQSTLIETLYRYRSDLQIIFNIIDSDHSGLISMEEFRAMWKLFNSHYSIHIDDFQIDDLAERMDLNKDGSIDFNEFLKAFYVVHKLDNVNKSDSKLA

Target ORF information:

RefSeq Version	XM_014111940.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111940.1

ORF Insert Sequence:

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_014111941.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967416.1 Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe45761
Clone ID Related Accession (Same CDS sequence)	XM_014111939.2 , XM_014111940.1 , XM_014111941.1 , XM_014111940.2 , XM_022415941.1 , XM_014111939.1
Accession Version	XM_014111941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967416.1
CDS	168..2153
Translation	MSTHDLEDMGCGTSSSTKDRKSEKVLKAALVIQNWYRGYSARLKARQRYALTIFQSIEYADEQGQLQLSNFFSFMLENYTQIHKKDPELLARLFGSTLQDYKDRQDYVSHIDVPDSYHGPRLQFPLTFTDIDLLVEAFKQQQILHAHYVLEVLFETKKVLKQMPNFTHVKTSPSKELTICGDLHGKLDDLFLIFYKNGLPSGSNTYIFNGDFVDRGKHSIEILMILFVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKIHGKKILQILEDVYTWLPIGTIIDSEILVIHGGISESTDLNLLHRIERNKMKSVLMSPIPLNEDHNNDLKKNKINTTIIPREVKTNGSLSEQLTKHEWEQVIDILWSDPRGKRGCYPNTSRGGGCYFGPDITSKVLNKYQLKMLIRSHECKPEGYEICHDGKVVTVFSASNYYEEGSNRGAYIKLCSGMNLRFFQYQVTKATCLRPLYQRVNTIESSAIRILKERMISRKTDLIHAFQLQDRSKSGKLPMGQWAFSMENVLGLNLPWRSLSPHLVTTDKSGNINYMSSFEDIHIQKPVKEAQSTLIETLYRYRSDLQIIFNIIDSDHSGLISMEEFRAMWKLFNSHYSIHIDDFQIDDLAERMDLNKDGSIDFNEFLKAFYVVHKLDNVNKSDSKLA

Target ORF information:

RefSeq Version	XM_014111941.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111941.1

ORF Insert Sequence:

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_014111940.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967415.1 Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45761
Clone ID Related Accession (Same CDS sequence)	XM_014111939.2 , XM_014111940.1 , XM_014111941.1 , XM_014111940.2 , XM_022415941.1 , XM_014111939.1
Accession Version	XM_014111940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967415.1
CDS	268..2253
Translation	MSTHDLEDMGCGTSSSTKDRKSEKVLKAALVIQNWYRGYSARLKARQRYALTIFQSIEYADEQGQLQLSNFFSFMLENYTQIHKKDPELLARLFGSTLQDYKDRQDYVSHIDVPDSYHGPRLQFPLTFTDIDLLVEAFKQQQILHAHYVLEVLFETKKVLKQMPNFTHVKTSPSKELTICGDLHGKLDDLFLIFYKNGLPSGSNTYIFNGDFVDRGKHSIEILMILFVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKIHGKKILQILEDVYTWLPIGTIIDSEILVIHGGISESTDLNLLHRIERNKMKSVLMSPIPLNEDHNNDLKKNKINTTIIPREVKTNGSLSEQLTKHEWEQVIDILWSDPRGKRGCYPNTSRGGGCYFGPDITSKVLNKYQLKMLIRSHECKPEGYEICHDGKVVTVFSASNYYEEGSNRGAYIKLCSGMNLRFFQYQVTKATCLRPLYQRVNTIESSAIRILKERMISRKTDLIHAFQLQDRSKSGKLPMGQWAFSMENVLGLNLPWRSLSPHLVTTDKSGNINYMSSFEDIHIQKPVKEAQSTLIETLYRYRSDLQIIFNIIDSDHSGLISMEEFRAMWKLFNSHYSIHIDDFQIDDLAERMDLNKDGSIDFNEFLKAFYVVHKLDNVNKSDSKLA

Target ORF information:

RefSeq Version	XM_014111940.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111940.2

ORF Insert Sequence:

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_022415941.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022271649.1 Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe45761
Clone ID Related Accession (Same CDS sequence)	XM_014111939.2 , XM_014111940.1 , XM_014111941.1 , XM_014111940.2 , XM_022415941.1 , XM_014111939.1
Accession Version	XM_022415941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271649.1
CDS	588..2573
Translation	MSTHDLEDMGCGTSSSTKDRKSEKVLKAALVIQNWYRGYSARLKARQRYALTIFQSIEYADEQGQLQLSNFFSFMLENYTQIHKKDPELLARLFGSTLQDYKDRQDYVSHIDVPDSYHGPRLQFPLTFTDIDLLVEAFKQQQILHAHYVLEVLFETKKVLKQMPNFTHVKTSPSKELTICGDLHGKLDDLFLIFYKNGLPSGSNTYIFNGDFVDRGKHSIEILMILFVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKIHGKKILQILEDVYTWLPIGTIIDSEILVIHGGISESTDLNLLHRIERNKMKSVLMSPIPLNEDHNNDLKKNKINTTIIPREVKTNGSLSEQLTKHEWEQVIDILWSDPRGKRGCYPNTSRGGGCYFGPDITSKVLNKYQLKMLIRSHECKPEGYEICHDGKVVTVFSASNYYEEGSNRGAYIKLCSGMNLRFFQYQVTKATCLRPLYQRVNTIESSAIRILKERMISRKTDLIHAFQLQDRSKSGKLPMGQWAFSMENVLGLNLPWRSLSPHLVTTDKSGNINYMSSFEDIHIQKPVKEAQSTLIETLYRYRSDLQIIFNIIDSDHSGLISMEEFRAMWKLFNSHYSIHIDDFQIDDLAERMDLNKDGSIDFNEFLKAFYVVHKLDNVNKSDSKLA

Target ORF information:

RefSeq Version	XM_022415941.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415941.1

ORF Insert Sequence:

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_014111939.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967414.1 Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA.

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CloneID	OCe45761
Clone ID Related Accession (Same CDS sequence)	XM_014111939.2 , XM_014111940.1 , XM_014111941.1 , XM_014111940.2 , XM_022415941.1 , XM_014111939.1
Accession Version	XM_014111939.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967414.1
CDS	507..2492
Translation	MSTHDLEDMGCGTSSSTKDRKSEKVLKAALVIQNWYRGYSARLKARQRYALTIFQSIEYADEQGQLQLSNFFSFMLENYTQIHKKDPELLARLFGSTLQDYKDRQDYVSHIDVPDSYHGPRLQFPLTFTDIDLLVEAFKQQQILHAHYVLEVLFETKKVLKQMPNFTHVKTSPSKELTICGDLHGKLDDLFLIFYKNGLPSGSNTYIFNGDFVDRGKHSIEILMILFVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKIHGKKILQILEDVYTWLPIGTIIDSEILVIHGGISESTDLNLLHRIERNKMKSVLMSPIPLNEDHNNDLKKNKINTTIIPREVKTNGSLSEQLTKHEWEQVIDILWSDPRGKRGCYPNTSRGGGCYFGPDITSKVLNKYQLKMLIRSHECKPEGYEICHDGKVVTVFSASNYYEEGSNRGAYIKLCSGMNLRFFQYQVTKATCLRPLYQRVNTIESSAIRILKERMISRKTDLIHAFQLQDRSKSGKLPMGQWAFSMENVLGLNLPWRSLSPHLVTTDKSGNINYMSSFEDIHIQKPVKEAQSTLIETLYRYRSDLQIIFNIIDSDHSGLISMEEFRAMWKLFNSHYSIHIDDFQIDDLAERMDLNKDGSIDFNEFLKAFYVVHKLDNVNKSDSKLA

Target ORF information:

RefSeq Version	XM_014111939.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111939.1

ORF Insert Sequence:

ATGAGCACAC ATGATTTAGA AGACATGGGC TGTGGCACCT CGTCTTCAAC GAAAGACAGA 
AAATCTGAGA AAGTGCTAAA AGCCGCATTG GTCATCCAGA ACTGGTACCG AGGTTACAGT 
GCTCGGTTGA AGGCCAGACA ACGCTATGCC CTCACCATCT TCCAGTCTAT CGAATATGCT 
GATGAACAAG GCCAATTGCA GCTCTCCAAC TTCTTTTCCT TCATGTTGGA AAACTACACA 
CAAATACACA AGAAAGATCC AGAATTACTT GCTCGACTTT TTGGTAGCAC CCTCCAGGAC 
TACAAGGATA GACAGGACTA TGTCTCACAC ATAGACGTCC CAGACTCCTA TCATGGTCCT 
CGGCTGCAAT TTCCTCTCAC TTTTACTGAT ATTGATTTAC TTGTTGAGGC CTTCAAGCAA 
CAACAGATAC TTCATGCTCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAGGTCCTG 
AAGCAAATGC CAAACTTCAC TCACGTAAAA ACTTCTCCCT CCAAAGAGTT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTATAAGAA TGGTCTCCCC 
TCAGGGAGCA ACACATATAT TTTTAATGGT GATTTTGTAG ATCGAGGAAA ACATTCCATA 
GAGATCCTAA TGATCCTGTT TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ATGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACAAA AGAAATTTTG 
CACAAATATA AGATACATGG GAAAAAAATC TTACAAATCT TGGAAGATGT CTATACCTGG 
CTCCCAATTG GTACAATCAT TGACAGTGAA ATCCTGGTCA TCCATGGTGG TATATCAGAG 
TCCACAGACT TGAACTTACT CCACCGTATA GAAAGAAACA AAATGAAATC TGTGCTGATG 
TCACCAATTC CCTTAAATGA AGACCACAAT AATGACTTGA AGAAAAATAA GATAAATACA 
ACTATTATAC CTCGGGAAGT CAAAACAAAT GGATCCCTTT CTGAACAGTT AACAAAGCAT 
GAATGGGAGC AGGTTATCGA TATTCTATGG AGTGATCCCA GAGGCAAAAG AGGCTGTTAC 
CCAAACACAA GTCGAGGAGG GGGCTGCTAC TTTGGACCTG ATATTACCTC CAAGGTTCTT 
AATAAATACC AGTTGAAGAT GCTCATTAGG TCTCATGAAT GTAAGCCCGA AGGGTATGAA 
ATCTGCCATG ATGGGAAGGT TGTCACTGTA TTTTCTGCTT CTAATTATTA TGAAGAAGGC 
AGCAATCGAG GAGCTTACAT CAAACTGTGC AGTGGTATGA ACCTTCGATT TTTCCAGTAC 
CAAGTAACTA AGGCAACGTG CTTGAGGCCT CTTTACCAAA GGGTGAATAC TATTGAAAGT 
AGTGCCATCA GGATATTAAA AGAGAGAATG ATTTCACGAA AAACTGATCT CATTCATGCT 
TTCCAACTTC AAGACCGCAG TAAATCAGGA AAACTTCCGA TGGGCCAGTG GGCTTTTTCC 
ATGGAGAACG TTTTGGGACT GAACTTACCA TGGAGATCCC TGAGTCCACA TCTGGTAACC 
ACAGACAAAA GTGGAAATAT CAACTACATG TCCAGCTTCG AGGATATCCA CATTCAGAAA 
CCTGTGAAAG AGGCTCAATC GACTCTAATT GAAACTCTGT ACAGATACCG ATCTGACCTG 
CAAATCATCT TTAACATCAT TGACTCTGAT CACTCAGGCC TGATTTCCAT GGAAGAATTT 
CGTGCCATGT GGAAACTCTT CAATAGTCAC TACAGCATTC ATATTGATGA CTTTCAAATT 
GATGACCTCG CCGAAAGAAT GGACTTAAAC AAAGATGGAA GCATTGACTT TAATGAGTTT 
CTAAAGGCTT TCTATGTAGT ACATAAATTG GATAATGTGA ACAAATCAGA TAGCAAACTT 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PPEF1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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