SEMA5B cDNA ORF clone, Canis lupus familiaris(Dog)

The following SEMA5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe30304 XM_022413758.1
Latest version!
Canis lupus familiaris semaphorin 5B (SEMA5B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30304 XM_005639649.2
Latest version!
Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe30304
    Clone ID Related Accession (Same CDS sequence) XM_022413758.1 , XM_005639649.2
    Accession Version XM_022413758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: semaphorin-5B
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTGTTCC CCAGACCCCT GGCTGTCACC CTGTTGCCGC CCAGCCTCAC GCTGCTGGTG 
    GTCCACCTGT CCAGCTCCCA GGATGTCGCC ACCGAACCCA GCAGTGAGCA GCAGCTGTGC
    ACGCTGAGGG AACACCCCAC CGTGGCCTTT GCGGATCTGA AGCCGTGGGT CTTTAACTTC
    ACCTACCCTG GAGCCCGAGA TTTCTCCCAG CTTGCTCTGG ATCCCCCCAG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTTTC CCTCCTTCAG
    GCCACAGAGT GGGCCTCCAA CGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGGGTC CTGATTGTCA CTGGCCGGAA AGTGTTCCTG
    TGTGGGACCA ATGCCTTTTC CCCCGTGTGC TCCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACCATAGAGA AGATCAATGG TGTGGCCCGC TGCCCCTACG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCTCAGGG GGAGCTCTAT GCGGCCACCG TCATTGACTT CTCAGGTCGG
    GACCCCGCCA TCTACCGCAG CCTGGGCAGC GGGCCACCAC TTCGCACCGC CCAGTACAAC
    TCCAAGTGGC TCAACGAGCC AAACTTTGTG GCAGCCTATG ATATCGGGCT CTTCGCCTAC
    TTCTTCCTGC GGGAGAACGC GGTGGAGCAC GACTGCGGGC GCACGGTGTA CTCTCGCGTG
    GCCCGAGTGT GCAAGAACGA CGTGGGAGGC CGCTTCCTGC TGGAGGACAC GTGGACCACG
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTACAACGAG
    CTGCAGAGTG CCTTCCACCT GCCGGAGCAG GACCTCATCT ACGGGGTCTT CACCACCAAC
    GTAAACAGCA TCGCCGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCCAGGCT
    TTCAATGGCC CATTTCGATA CCAGGAGAAC CCCAGGGCCG CCTGGCTCCC CATCGCTAAC
    CCCATCCCCA ACTTCCAGTG CGGCACCCTG CCAGAGGTGG GCCCCAACGA GAACCTGACA
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTG TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACCCCTGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTT
    GTGCAGGCCA AGGACACGCT CTACCACGTG CTCTACATCG GCACAGAGTC GGGCACCATC
    CTGAAGGCCC TGTCCACGAC GAGCCGCAGC CTCCGCGGCT GCTACCTGGA GGAGCTGCAC
    GTCCTGCCCC CTGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTTG TGGGGCTGAG CGACGGCGTG CTGCGGGTGC CGCTGGAGAG GTGCGCGGCC
    TACCGCAGCC AGGGGGCGTG CCTAGGGGCC CGGGACCCAT ACTGCGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT GGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATA
    ACAGCGTGTC CTGTGCGAAA TGTGACTCGG GATGGGGGCT TTGGCCAGTG GTCACCATGG
    CAACCATGTG AACACTTAGA TGGGGATAAT TCCGGCTCTT GCCTTTGTCG GGCCCGAGCC
    TGTGACTCCC CCCGACCCCG CTGTGGGGGC CGAGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGTT CCAGGAACGG GGCGTGGACC CCGTGGTCCC CGTGGGCCCC GTGCAGCACG
    TCCTGCGGGA TCGGATTCCA GGTCCGCCAG CGAAGCTGCA GCAACCCCGC TCCCCGCCAC
    GGAGGCCGCA TCTGCGTGGG CAAGAGCCGC GAGGAGCGGT TCTGCAACGA GAACGCGCCC
    TGCCCGGTGC CCATATTCTG GGCGTCCTGG GGCTCCTGGA GCAAGTGCAG GCTGGCGGGG
    GGGCCGGCCC TGCACCCCGG GGCGGGCCTG CGGGGGGTGG GCCCGCGCCT GCAGGTCGGC
    AGCCCAGCGC CCGCTCCCCT CCCGAAGGAG GTCGAGACGT GCAACCCCGA GGGCTGCCTC
    TAAGTGCGGC GCAACACCCC GTGGACGCCG TGGCTGCCGG TGAACGTGAC GCAAGCTGGA
    GTGCGGCAGG AGCAGCGTTT CCGCTTCACG TGACGCGTGC CGCTTCCCCG GGGCGGTGGC
    GGGGTGCCCG GGGCGTGGCG GGAGCCCGCG CTGACGCGTC CCCCCGCAGC CCTGGTGGAG
    GACCTCCTGC GCGGCGGCAG CACCTCCCCG CGCTCGCTGA GCGGCGGCTG GGCGGCGTGG
    GGCGCGTGGT CGTCCTGCTC CCGGGCCTGC GAGCTGGGCT TCCGCGTCCG CAAGCGCACG
    TGCACGAACC CCGAGCCCCG CAGCGGAGGC CTCCCGTGCA GCGGCGACGC GGCCGAGTAC
    CAGGACTGCA ACCCCCAGGC CTGTCCAGTG CGGGGTGCCT GGTCCTGCTG GACCCCATGG
    TCCCCCTGCT CAGCCTCCTG TGGGGGAGGC CACTACCAAC GCACCCGATC CTGCACCAGC
    CCCGCGCCCT CCCCAGGCGA GGACATCTGT CTGGGTCTGC ACACAGAGGA GGCCCTGTGT
    GCCACACAGG CTTGCCCGGA AGGCTGGTCG CCGTGGTCCG AGTGGAGCGC GTGCACTGAG
    GAGGGAGCCC AGAGCCGCAG CAGGCACTGT GAAGACCTGG TCCCGGGGCC CAGCACCTGT
    GCTGGGAATA GCAGCCAGAG CCGCCCCTGT CCCTACAGCG AGATTCCTGT GATCCTGCCT
    GCCTCTAGCA TGGAGGAGGC CACCAGCTGT GGAGGCTTCA GTCTCATCCA CCTGGTAGCC
    ACAGGTGTCT CCTGCTTCCT GGGCTCTGGG CTGCTGACCT TGGCAGTGTA CCTGTCCTGC
    CAGCACTGCC AGCGCCAGTC CCAGGAATCT ACACTCGTCC ATCCGGCCAC CCCCAACCAC
    CTGCACTACA AGGGCGGGGG CACCCCCAAG AACGAGAAGT ACACACCCAT GGAGTTCAAG
    ACGCTGAACA AGAATAACCT GATCCCCGAT GACAGAGCCA ACTTCTACCC ATTGCAGCAG
    ACCAACGTGT ACACAACCAC CTACTATCCC AGCCCACTGA ACAAGCACAG CTTCCGGCCC
    GAGGCCTCAC CTGGACAACG GTGCTTCCCC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269466.1
    CDS733..4011
    Translation

    Target ORF information:

    RefSeq Version XM_022413758.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5B (SEMA5B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTGTTCC CCAGACCCCT GGCTGTCACC CTGTTGCCGC CCAGCCTCAC GCTGCTGGTG 
    GTCCACCTGT CCAGCTCCCA GGATGTCGCC ACCGAACCCA GCAGTGAGCA GCAGCTGTGC
    ACGCTGAGGG AACACCCCAC CGTGGCCTTT GCGGATCTGA AGCCGTGGGT CTTTAACTTC
    ACCTACCCTG GAGCCCGAGA TTTCTCCCAG CTTGCTCTGG ATCCCCCCAG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTTTC CCTCCTTCAG
    GCCACAGAGT GGGCCTCCAA CGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGGGTC CTGATTGTCA CTGGCCGGAA AGTGTTCCTG
    TGTGGGACCA ATGCCTTTTC CCCCGTGTGC TCCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACCATAGAGA AGATCAATGG TGTGGCCCGC TGCCCCTACG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCTCAGGG GGAGCTCTAT GCGGCCACCG TCATTGACTT CTCAGGTCGG
    GACCCCGCCA TCTACCGCAG CCTGGGCAGC GGGCCACCAC TTCGCACCGC CCAGTACAAC
    TCCAAGTGGC TCAACGAGCC AAACTTTGTG GCAGCCTATG ATATCGGGCT CTTCGCCTAC
    TTCTTCCTGC GGGAGAACGC GGTGGAGCAC GACTGCGGGC GCACGGTGTA CTCTCGCGTG
    GCCCGAGTGT GCAAGAACGA CGTGGGAGGC CGCTTCCTGC TGGAGGACAC GTGGACCACG
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTACAACGAG
    CTGCAGAGTG CCTTCCACCT GCCGGAGCAG GACCTCATCT ACGGGGTCTT CACCACCAAC
    GTAAACAGCA TCGCCGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCCAGGCT
    TTCAATGGCC CATTTCGATA CCAGGAGAAC CCCAGGGCCG CCTGGCTCCC CATCGCTAAC
    CCCATCCCCA ACTTCCAGTG CGGCACCCTG CCAGAGGTGG GCCCCAACGA GAACCTGACA
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTG TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACCCCTGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTT
    GTGCAGGCCA AGGACACGCT CTACCACGTG CTCTACATCG GCACAGAGTC GGGCACCATC
    CTGAAGGCCC TGTCCACGAC GAGCCGCAGC CTCCGCGGCT GCTACCTGGA GGAGCTGCAC
    GTCCTGCCCC CTGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTTG TGGGGCTGAG CGACGGCGTG CTGCGGGTGC CGCTGGAGAG GTGCGCGGCC
    TACCGCAGCC AGGGGGCGTG CCTAGGGGCC CGGGACCCAT ACTGCGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT GGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATA
    ACAGCGTGTC CTGTGCGAAA TGTGACTCGG GATGGGGGCT TTGGCCAGTG GTCACCATGG
    CAACCATGTG AACACTTAGA TGGGGATAAT TCCGGCTCTT GCCTTTGTCG GGCCCGAGCC
    TGTGACTCCC CCCGACCCCG CTGTGGGGGC CGAGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGTT CCAGGAACGG GGCGTGGACC CCGTGGTCCC CGTGGGCCCC GTGCAGCACG
    TCCTGCGGGA TCGGATTCCA GGTCCGCCAG CGAAGCTGCA GCAACCCCGC TCCCCGCCAC
    GGAGGCCGCA TCTGCGTGGG CAAGAGCCGC GAGGAGCGGT TCTGCAACGA GAACGCGCCC
    TGCCCGGTGC CCATATTCTG GGCGTCCTGG GGCTCCTGGA GCAAGTGCAG GCTGGCGGGG
    GGGCCGGCCC TGCACCCCGG GGCGGGCCTG CGGGGGGTGG GCCCGCGCCT GCAGGTCGGC
    AGCCCAGCGC CCGCTCCCCT CCCGAAGGAG GTCGAGACGT GCAACCCCGA GGGCTGCCTC
    TAAGTGCGGC GCAACACCCC GTGGACGCCG TGGCTGCCGG TGAACGTGAC GCAAGCTGGA
    GTGCGGCAGG AGCAGCGTTT CCGCTTCACG TGACGCGTGC CGCTTCCCCG GGGCGGTGGC
    GGGGTGCCCG GGGCGTGGCG GGAGCCCGCG CTGACGCGTC CCCCCGCAGC CCTGGTGGAG
    GACCTCCTGC GCGGCGGCAG CACCTCCCCG CGCTCGCTGA GCGGCGGCTG GGCGGCGTGG
    GGCGCGTGGT CGTCCTGCTC CCGGGCCTGC GAGCTGGGCT TCCGCGTCCG CAAGCGCACG
    TGCACGAACC CCGAGCCCCG CAGCGGAGGC CTCCCGTGCA GCGGCGACGC GGCCGAGTAC
    CAGGACTGCA ACCCCCAGGC CTGTCCAGTG CGGGGTGCCT GGTCCTGCTG GACCCCATGG
    TCCCCCTGCT CAGCCTCCTG TGGGGGAGGC CACTACCAAC GCACCCGATC CTGCACCAGC
    CCCGCGCCCT CCCCAGGCGA GGACATCTGT CTGGGTCTGC ACACAGAGGA GGCCCTGTGT
    GCCACACAGG CTTGCCCGGA AGGCTGGTCG CCGTGGTCCG AGTGGAGCGC GTGCACTGAG
    GAGGGAGCCC AGAGCCGCAG CAGGCACTGT GAAGACCTGG TCCCGGGGCC CAGCACCTGT
    GCTGGGAATA GCAGCCAGAG CCGCCCCTGT CCCTACAGCG AGATTCCTGT GATCCTGCCT
    GCCTCTAGCA TGGAGGAGGC CACCAGCTGT GGAGGCTTCA GTCTCATCCA CCTGGTAGCC
    ACAGGTGTCT CCTGCTTCCT GGGCTCTGGG CTGCTGACCT TGGCAGTGTA CCTGTCCTGC
    CAGCACTGCC AGCGCCAGTC CCAGGAATCT ACACTCGTCC ATCCGGCCAC CCCCAACCAC
    CTGCACTACA AGGGCGGGGG CACCCCCAAG AACGAGAAGT ACACACCCAT GGAGTTCAAG
    ACGCTGAACA AGAATAACCT GATCCCCGAT GACAGAGCCA ACTTCTACCC ATTGCAGCAG
    ACCAACGTGT ACACAACCAC CTACTATCCC AGCCCACTGA ACAAGCACAG CTTCCGGCCC
    GAGGCCTCAC CTGGACAACG GTGCTTCCCC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30304
    Clone ID Related Accession (Same CDS sequence) XM_022413758.1 , XM_005639649.2
    Accession Version XM_005639649.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LOW QUALITY PROTEIN: semaphorin-5B
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTGTTCC CCAGACCCCT GGCTGTCACC CTGTTGCCGC CCAGCCTCAC GCTGCTGGTG 
    GTCCACCTGT CCAGCTCCCA GGATGTCGCC ACCGAACCCA GCAGTGAGCA GCAGCTGTGC
    ACGCTGAGGG AACACCCCAC CGTGGCCTTT GCGGATCTGA AGCCGTGGGT CTTTAACTTC
    ACCTACCCTG GAGCCCGAGA TTTCTCCCAG CTTGCTCTGG ATCCCCCCAG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTTTC CCTCCTTCAG
    GCCACAGAGT GGGCCTCCAA CGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGGGTC CTGATTGTCA CTGGCCGGAA AGTGTTCCTG
    TGTGGGACCA ATGCCTTTTC CCCCGTGTGC TCCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACCATAGAGA AGATCAATGG TGTGGCCCGC TGCCCCTACG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCTCAGGG GGAGCTCTAT GCGGCCACCG TCATTGACTT CTCAGGTCGG
    GACCCCGCCA TCTACCGCAG CCTGGGCAGC GGGCCACCAC TTCGCACCGC CCAGTACAAC
    TCCAAGTGGC TCAACGAGCC AAACTTTGTG GCAGCCTATG ATATCGGGCT CTTCGCCTAC
    TTCTTCCTGC GGGAGAACGC GGTGGAGCAC GACTGCGGGC GCACGGTGTA CTCTCGCGTG
    GCCCGAGTGT GCAAGAACGA CGTGGGAGGC CGCTTCCTGC TGGAGGACAC GTGGACCACG
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTACAACGAG
    CTGCAGAGTG CCTTCCACCT GCCGGAGCAG GACCTCATCT ACGGGGTCTT CACCACCAAC
    GTAAACAGCA TCGCCGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCCAGGCT
    TTCAATGGCC CATTTCGATA CCAGGAGAAC CCCAGGGCCG CCTGGCTCCC CATCGCTAAC
    CCCATCCCCA ACTTCCAGTG CGGCACCCTG CCAGAGGTGG GCCCCAACGA GAACCTGACA
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTG TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACCCCTGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTT
    GTGCAGGCCA AGGACACGCT CTACCACGTG CTCTACATCG GCACAGAGTC GGGCACCATC
    CTGAAGGCCC TGTCCACGAC GAGCCGCAGC CTCCGCGGCT GCTACCTGGA GGAGCTGCAC
    GTCCTGCCCC CTGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTTG TGGGGCTGAG CGACGGCGTG CTGCGGGTGC CGCTGGAGAG GTGCGCGGCC
    TACCGCAGCC AGGGGGCGTG CCTAGGGGCC CGGGACCCAT ACTGCGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT GGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATA
    ACAGCGTGTC CTGTGCGAAA TGTGACTCGG GATGGGGGCT TTGGCCAGTG GTCACCATGG
    CAACCATGTG AACACTTAGA TGGGGATAAT TCCGGCTCTT GCCTTTGTCG GGCCCGAGCC
    TGTGACTCCC CCCGACCCCG CTGTGGGGGC CGAGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGTT CCAGGAACGG GGCGTGGACC CCGTGGTCCC CGTGGGCCCC GTGCAGCACG
    TCCTGCGGGA TCGGATTCCA GGTCCGCCAG CGAAGCTGCA GCAACCCCGC TCCCCGCCAC
    GGAGGCCGCA TCTGCGTGGG CAAGAGCCGC GAGGAGCGGT TCTGCAACGA GAACGCGCCC
    TGCCCGGTGC CCATATTCTG GGCGTCCTGG GGCTCCTGGA GCAAGTGCAG GCTGGCGGGG
    GGGCCGGCCC TGCACCCCGG GGCGGGCCTG CGGGGGGTGG GCCCGCGCCT GCAGGTCGGC
    AGCCCAGCGC CCGCTCCCCT CCCGAAGGAG GTCGAGACGT GCAACCCCGA GGGCTGCCTC
    TAAGTGCGGC GCAACACCCC GTGGACGCCG TGGCTGCCGG TGAACGTGAC GCAAGCTGGA
    GTGCGGCAGG AGCAGCGTTT CCGCTTCACG TGACGCGTGC CGCTTCCCCG GGGCGGTGGC
    GGGGTGCCCG GGGCGTGGCG GGAGCCCGCG CTGACGCGTC CCCCCGCAGC CCTGGTGGAG
    GACCTCCTGC GCGGCGGCAG CACCTCCCCG CGCTCGCTGA GCGGCGGCTG GGCGGCGTGG
    GGCGCGTGGT CGTCCTGCTC CCGGGCCTGC GAGCTGGGCT TCCGCGTCCG CAAGCGCACG
    TGCACGAACC CCGAGCCCCG CAGCGGAGGC CTCCCGTGCA GCGGCGACGC GGCCGAGTAC
    CAGGACTGCA ACCCCCAGGC CTGTCCAGTG CGGGGTGCCT GGTCCTGCTG GACCCCATGG
    TCCCCCTGCT CAGCCTCCTG TGGGGGAGGC CACTACCAAC GCACCCGATC CTGCACCAGC
    CCCGCGCCCT CCCCAGGCGA GGACATCTGT CTGGGTCTGC ACACAGAGGA GGCCCTGTGT
    GCCACACAGG CTTGCCCGGA AGGCTGGTCG CCGTGGTCCG AGTGGAGCGC GTGCACTGAG
    GAGGGAGCCC AGAGCCGCAG CAGGCACTGT GAAGACCTGG TCCCGGGGCC CAGCACCTGT
    GCTGGGAATA GCAGCCAGAG CCGCCCCTGT CCCTACAGCG AGATTCCTGT GATCCTGCCT
    GCCTCTAGCA TGGAGGAGGC CACCAGCTGT GGAGGCTTCA GTCTCATCCA CCTGGTAGCC
    ACAGGTGTCT CCTGCTTCCT GGGCTCTGGG CTGCTGACCT TGGCAGTGTA CCTGTCCTGC
    CAGCACTGCC AGCGCCAGTC CCAGGAATCT ACACTCGTCC ATCCGGCCAC CCCCAACCAC
    CTGCACTACA AGGGCGGGGG CACCCCCAAG AACGAGAAGT ACACACCCAT GGAGTTCAAG
    ACGCTGAACA AGAATAACCT GATCCCCGAT GACAGAGCCA ACTTCTACCC ATTGCAGCAG
    ACCAACGTGT ACACAACCAC CTACTATCCC AGCCCACTGA ACAAGCACAG CTTCCGGCCC
    GAGGCCTCAC CTGGACAACG GTGCTTCCCC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639706.1
    CDS873..4151
    Translation

    Target ORF information:

    RefSeq Version XM_005639649.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTGTTCC CCAGACCCCT GGCTGTCACC CTGTTGCCGC CCAGCCTCAC GCTGCTGGTG 
    GTCCACCTGT CCAGCTCCCA GGATGTCGCC ACCGAACCCA GCAGTGAGCA GCAGCTGTGC
    ACGCTGAGGG AACACCCCAC CGTGGCCTTT GCGGATCTGA AGCCGTGGGT CTTTAACTTC
    ACCTACCCTG GAGCCCGAGA TTTCTCCCAG CTTGCTCTGG ATCCCCCCAG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTTTC CCTCCTTCAG
    GCCACAGAGT GGGCCTCCAA CGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGGGTC CTGATTGTCA CTGGCCGGAA AGTGTTCCTG
    TGTGGGACCA ATGCCTTTTC CCCCGTGTGC TCCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACCATAGAGA AGATCAATGG TGTGGCCCGC TGCCCCTACG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCTCAGGG GGAGCTCTAT GCGGCCACCG TCATTGACTT CTCAGGTCGG
    GACCCCGCCA TCTACCGCAG CCTGGGCAGC GGGCCACCAC TTCGCACCGC CCAGTACAAC
    TCCAAGTGGC TCAACGAGCC AAACTTTGTG GCAGCCTATG ATATCGGGCT CTTCGCCTAC
    TTCTTCCTGC GGGAGAACGC GGTGGAGCAC GACTGCGGGC GCACGGTGTA CTCTCGCGTG
    GCCCGAGTGT GCAAGAACGA CGTGGGAGGC CGCTTCCTGC TGGAGGACAC GTGGACCACG
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTACAACGAG
    CTGCAGAGTG CCTTCCACCT GCCGGAGCAG GACCTCATCT ACGGGGTCTT CACCACCAAC
    GTAAACAGCA TCGCCGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCCAGGCT
    TTCAATGGCC CATTTCGATA CCAGGAGAAC CCCAGGGCCG CCTGGCTCCC CATCGCTAAC
    CCCATCCCCA ACTTCCAGTG CGGCACCCTG CCAGAGGTGG GCCCCAACGA GAACCTGACA
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTG TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACCCCTGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTT
    GTGCAGGCCA AGGACACGCT CTACCACGTG CTCTACATCG GCACAGAGTC GGGCACCATC
    CTGAAGGCCC TGTCCACGAC GAGCCGCAGC CTCCGCGGCT GCTACCTGGA GGAGCTGCAC
    GTCCTGCCCC CTGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTTG TGGGGCTGAG CGACGGCGTG CTGCGGGTGC CGCTGGAGAG GTGCGCGGCC
    TACCGCAGCC AGGGGGCGTG CCTAGGGGCC CGGGACCCAT ACTGCGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT GGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATA
    ACAGCGTGTC CTGTGCGAAA TGTGACTCGG GATGGGGGCT TTGGCCAGTG GTCACCATGG
    CAACCATGTG AACACTTAGA TGGGGATAAT TCCGGCTCTT GCCTTTGTCG GGCCCGAGCC
    TGTGACTCCC CCCGACCCCG CTGTGGGGGC CGAGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGTT CCAGGAACGG GGCGTGGACC CCGTGGTCCC CGTGGGCCCC GTGCAGCACG
    TCCTGCGGGA TCGGATTCCA GGTCCGCCAG CGAAGCTGCA GCAACCCCGC TCCCCGCCAC
    GGAGGCCGCA TCTGCGTGGG CAAGAGCCGC GAGGAGCGGT TCTGCAACGA GAACGCGCCC
    TGCCCGGTGC CCATATTCTG GGCGTCCTGG GGCTCCTGGA GCAAGTGCAG GCTGGCGGGG
    GGGCCGGCCC TGCACCCCGG GGCGGGCCTG CGGGGGGTGG GCCCGCGCCT GCAGGTCGGC
    AGCCCAGCGC CCGCTCCCCT CCCGAAGGAG GTCGAGACGT GCAACCCCGA GGGCTGCCTC
    TAAGTGCGGC GCAACACCCC GTGGACGCCG TGGCTGCCGG TGAACGTGAC GCAAGCTGGA
    GTGCGGCAGG AGCAGCGTTT CCGCTTCACG TGACGCGTGC CGCTTCCCCG GGGCGGTGGC
    GGGGTGCCCG GGGCGTGGCG GGAGCCCGCG CTGACGCGTC CCCCCGCAGC CCTGGTGGAG
    GACCTCCTGC GCGGCGGCAG CACCTCCCCG CGCTCGCTGA GCGGCGGCTG GGCGGCGTGG
    GGCGCGTGGT CGTCCTGCTC CCGGGCCTGC GAGCTGGGCT TCCGCGTCCG CAAGCGCACG
    TGCACGAACC CCGAGCCCCG CAGCGGAGGC CTCCCGTGCA GCGGCGACGC GGCCGAGTAC
    CAGGACTGCA ACCCCCAGGC CTGTCCAGTG CGGGGTGCCT GGTCCTGCTG GACCCCATGG
    TCCCCCTGCT CAGCCTCCTG TGGGGGAGGC CACTACCAAC GCACCCGATC CTGCACCAGC
    CCCGCGCCCT CCCCAGGCGA GGACATCTGT CTGGGTCTGC ACACAGAGGA GGCCCTGTGT
    GCCACACAGG CTTGCCCGGA AGGCTGGTCG CCGTGGTCCG AGTGGAGCGC GTGCACTGAG
    GAGGGAGCCC AGAGCCGCAG CAGGCACTGT GAAGACCTGG TCCCGGGGCC CAGCACCTGT
    GCTGGGAATA GCAGCCAGAG CCGCCCCTGT CCCTACAGCG AGATTCCTGT GATCCTGCCT
    GCCTCTAGCA TGGAGGAGGC CACCAGCTGT GGAGGCTTCA GTCTCATCCA CCTGGTAGCC
    ACAGGTGTCT CCTGCTTCCT GGGCTCTGGG CTGCTGACCT TGGCAGTGTA CCTGTCCTGC
    CAGCACTGCC AGCGCCAGTC CCAGGAATCT ACACTCGTCC ATCCGGCCAC CCCCAACCAC
    CTGCACTACA AGGGCGGGGG CACCCCCAAG AACGAGAAGT ACACACCCAT GGAGTTCAAG
    ACGCTGAACA AGAATAACCT GATCCCCGAT GACAGAGCCA ACTTCTACCC ATTGCAGCAG
    ACCAACGTGT ACACAACCAC CTACTATCCC AGCCCACTGA ACAAGCACAG CTTCCGGCCC
    GAGGCCTCAC CTGGACAACG GTGCTTCCCC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.