PLCH1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014106734.1	XP_013962209.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_022408773.1	XP_022264481.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_022408775.1	XP_022264483.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_014106729.2	XP_013962204.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_014106727.1	XP_013962202.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_014106728.1	XP_013962203.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_014106731.1	XP_013962206.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_014106733.1	XP_013962208.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_005634601.2	XP_005634658.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_014106727.2	XP_013962202.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_022408777.1	XP_022264485.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_005634594.2	XP_005634651.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_014106732.1	XP_013962207.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_014106729.1	XP_013962204.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_022408774.1	XP_022264482.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_005634595.2	XP_005634652.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_022408776.1	XP_022264484.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_014106726.1	XP_013962201.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_005634593.2	XP_005634650.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_005634595.1	XP_005634652.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_014106726.2	XP_013962201.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe24334	XM_005634594.2 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24334	XM_005634593.2 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24334	XM_022408774.1 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24334	XM_022408773.1 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24334	XM_014106726.2 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24335	XM_005634595.2 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24336	XM_014106731.1 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24336	XM_022408776.1 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24338	XM_014106733.1 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe24338	XM_014106732.1 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe24338	XM_005634601.2 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe24338	XM_014106734.1 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe296576	XM_022408777.1 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe42578	XM_014106727.2 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe42579	XM_014106728.1 Latest version!	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe42579	XM_022408775.1 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe42580	XM_014106729.2 Latest version!	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OCe42578	XM_014106727.1	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe42580	XM_014106729.1	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24335	XM_005634595.1	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe24334	XM_014106726.1	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

PLCH1 ( XM_022408773.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264481.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe24334
Clone ID Related Accession (Same CDS sequence)	XM_022408773.1 , XM_005634594.2 , XM_022408774.1 , XM_014106726.1 , XM_005634593.2 , XM_014106726.2
Accession Version	XM_022408773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264481.1
CDS	201..5270
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_022408773.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408773.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005634594.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634651.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OCe24334
Clone ID Related Accession (Same CDS sequence)	XM_022408773.1 , XM_005634594.2 , XM_022408774.1 , XM_014106726.1 , XM_005634593.2 , XM_014106726.2
Accession Version	XM_005634594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634651.1
CDS	62..5131
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005634594.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634594.2

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_022408774.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264482.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OCe24334
Clone ID Related Accession (Same CDS sequence)	XM_022408773.1 , XM_005634594.2 , XM_022408774.1 , XM_014106726.1 , XM_005634593.2 , XM_014106726.2
Accession Version	XM_022408774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264482.1
CDS	232..5301
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_022408774.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408774.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106726.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962201.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24334
Clone ID Related Accession (Same CDS sequence)	XM_022408773.1 , XM_005634594.2 , XM_022408774.1 , XM_014106726.1 , XM_005634593.2 , XM_014106726.2
Accession Version	XM_014106726.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962201.1
CDS	248..5317
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106726.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106726.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005634593.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634650.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OCe24334
Clone ID Related Accession (Same CDS sequence)	XM_022408773.1 , XM_005634594.2 , XM_022408774.1 , XM_014106726.1 , XM_005634593.2 , XM_014106726.2
Accession Version	XM_005634593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634650.1
CDS	228..5297
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005634593.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634593.2

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106726.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962201.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OCe24334
Clone ID Related Accession (Same CDS sequence)	XM_022408773.1 , XM_005634594.2 , XM_022408774.1 , XM_014106726.1 , XM_005634593.2 , XM_014106726.2
Accession Version	XM_014106726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014106726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962201.1
CDS	199..5268
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106726.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106726.2

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005634595.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634652.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OCe24335
Clone ID Related Accession (Same CDS sequence)	XM_005634595.2 , XM_005634595.1
Accession Version	XM_005634595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CTGGGACTCA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTAGTCCGG 
CTCTTTTACC TGGATGAGCA TCGGACCCGC CTTCGATGGA GACCCTCGAG GAAGAGCGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTGACCG AGGGCCGGCA GTCTGAGATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAGG ACTCCCTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAGG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAACTG ATGCACAAGT TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TTTGTGTTTT TTACAAAATG ATGTCATTGA GACGGGACCT TTATTTGTTG 
CTCTTGAACT ACAGTGACAA GAAAGATCAC CTAACTCTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAG TAATGTGACA ACAGACTATT GTCTTGACAT CGTAAGAAAA 
TTTGAAGTTT CAGAAGAAAA TAAGGCAAAA AATGTTCTTG GCATAGAAGG GTTCACAAAT 
TTCATGCGTA GTCCTGCTTG TGACATATTT AACCCACTGC ACCATGAGGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACCGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA GGAGGGCTGT 
CGCTGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TACACTCTGA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCACGCC 
TTTGTGAAGA ACGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAACTGGA CCTGTCATCT 
GTAGACACCG GAGAGACCAA ACAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTATCATCTT GGGGATGATG CAGAGGAGGG GGAAGTTTCC 
GACGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTACAATAAT 
GGGACCACTG AGCATCAGGT GGAGTCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTGAAA 
GAATCTCAAA TCCGAGACAA GGAAGATCCC GATAGCTTCA CAGTGAGGGC GCTACTGAGG 
GCCACACACG AAGGCCTAAA TGCACACCTG AAGCAGCATC CAGATGTGAA GGAAAATGGG 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGCA GAAAACCACA 
AAATCACGAT CTAAGTCTTA CAGTACTGAC GATGAGGAGG ACACACAGCA GAATCCCGGC 
AAGGAAACCG GCCAGCTGTA CAGGTTGGGT CGGCGCAGGA AAACCATGAA GCTCTGCCGA 
GAGCTCTCTG ACTTGGTGGT GTATACAAAC TCCGTGGCCG CCCAGGACAT CGTGGATGAT 
GGAACCACAG GAAACGTGTT ATCATTTAGT GAAACAAGAG CACATCAGGT GGTTCAGCAG 
AAATCGGAGC AGTTCATGAT CTACAACCAA AAGCAGCTCA CGAGGATTTA CCCGTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCT CTGCCCTACT GGAACGCAGG CTGCCAGCTA 
GTGGCTCTGA ACTACCAGTC CGAAGGGCGA ATGATGCAAT TAAATCGAGC CAAATTCAAG 
ACCAATGGCA ATTGTGGCTA CGTCCTCAAA CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTACTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAGC AGCTCATCCT GAAAGTGATC 
AGTGGACAGC AGCTCCCCAA ACCTCCAGAC TCCATGTTCG GAGACCGAGG CGAGATCATT 
GATCCTTTCG TTGAAGTTGA AATTATTGGA TTGCCAGTAG ATTGCTGTAA AGATCAAACC 
CGTGTAGTAG ATGACAATGG ATTTAACCCT GTGTGGGAAG AAACCCTGAC GTTTACAGTA 
CACATGCCAG ACATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC GATTGGACGA 
GACTTCGTTG GACAAAGAAC TGTGACCTTC AGCAGCTTAG TGCCTGGCTA CCGGCACGTC 
TACTTGGAAG GACTGACAGA GGCATCGATA TTTGTCCACA TAACAATCAA TGAAATCTAT 
GGGAAGTGGA GCCCTTTAAT ACTCAACCCC AGTTATACTA TATTGCACTT TCTAGGAGCT 
ACAAAGAGCA GACAGCTCCA AGGTCTGAAG GGATTGTTCA ATAAGAATCC CAGGCATGGT 
TCTTCAGAAA ACAATTCCCA TTACGTTCGG AAACGATCAA TTGGAGATAG AATTCTACGC 
CGCACAGCCA GCGCTCCAGC CAAAGGCAGA AAGAAGAGCA AAATGGGCTT CCAAGAGATG 
GTAGAGATAA AGGATTCTGT ATCTGAGGCT GCGAGAGATC AGGATGGTGT CCTGAGGAGG 
ACCACACGGA GCTTGCAAGA GCGCCCTGTC TCTCTACCTG TTGACAAAAG TCTTCTGGGG 
GCCTTGTCAC TGCCCCTATC TGAAACAGCG AAAGACACTG AAGGAAAAGA AAACTCTCTG 
GCAGAAGATA AGGATGGAAG AAGAAAAGGG AAGGCAAGTA TAAAAGACCA ACACTTTCCA 
AATTTCAACA AAAAGTTATC CTCTTCCTCT AGCGCTCTCC TCCACAAAGA CCCCCGCCCA 
GGGGGCTCCG CTGTTTCTGT CGCCAGCGCA TCCAGCGCAT CCGTCACCGG GGAACAGCTG 
GGAGCCCCGG GCCTCCGGGG CGGGAGAACC AGATCACCTG CGCCAGGTGA CTGCCAGGAG 
CAGGAGTGTC CCCGCAAACC CCGCTCCCCA AGGCAGCATC TGGCTCTGGA ACCTGCACTT 
ACCGCAGCGC AAGGTGGTGT GAGCACCCAG GCACCTGCGC GCGCTGGAGG CCTTGGGGAG 
GACGGAAGCC CCCGGTCAGG AAGCAGGTTT TCTCAAAGCC ACCTGGAGGG CAGGGACTTG 
GAGGGCAGCT GGGTGACGGG CCGAGCCGCA GCGTCCCTCT CTCTGTCCGA CGTGTCTCTG 
CTGTGTGCTG CGCACCCCGA CTCGCACTCC GCGGCCGTTC TGCAGGAGAG CGACATCTCC 
CATCTTCTTG ACACTGTCCC TCTCGCTACC GAGGGCGACC CGGGCAGCTC CATCTCGGCG 
CTGGTCGGCC AGTGTGAGGA CGCCGGGGAG CCGGGGAGCC TCGCCGCGGC TCCTCCCCCG 
CACAGCACCT GCGTGGCGGC AGGTCATCCT CCCTTGCCCG CCCTGGACCC CGAGCCGGCC 
GTGGAGCCCG ACGCTGCCGC GGGAAAATGC AAGCCCTGCT TCGCGCGCGC ATCCCCCGCC 
CGGATTCCCG CCAAGGGCCC CGAGGCCTCG GCGCACACGC TTCACCAGAC CACCTGCGCC 
ACTGCCCCGC GGCCGCCCCC GGAGGACCAG CTCGCCGCGG GAAGCCACCG GCCCGGGGCC 
TCGGCCACGT GTCCCTGCCC GCCACCGCGA AGCCCCGGCG CCGGGCAGGT GCGAGGAGCG 
CCCGGGAGCC GCAGCCCCAC CCCCGCCCCG GCCCCCGCGG CCCCGCCAGC CGACCGCGCG 
CCCCGTTTCC ATCCTGGAGA CCGCAGTGTC GTGGGGGCGG GGGGCGCCGC AGGCCCCGCG 
GACCTGCCCG CACCTGCCGC TGAACGCCCC GGCGACGACG CAGGCCCCCT CGCGGCTCCT 
CTGCAGCCCA GGCGCAGCCC GCACGCGGTG GACCACGTCC CCGGAGCTCG GGGAAACGGC 
CGCTGCCCAG ACACCCCGCA CCCCCGCGTC CCCGCGGCGC CGCACAGCGC TCCCCAAAGC 
ACCTCTGGCC TCACCTGCCC AGGTGCTGCC GCTCCCACGC GCAGCCGCTG CTCGCACCCG 
GATGCCCACG CCGAGCAGGC CCGCGTGCCC GCGCCCCGCC ACGCGCCCCC GGGGCCCGCC 
CTCAAGCTGC CCAGCCCTTG CAAGTCCAAG AGCCTGGGCG ACCTGACATC CGAGGACATC 
GCCTGCAACT TCGAAAGCAA GTACCAGTGC ATCAGCAGGA GCTTTGTCGC CACCGGCCTC 
CGCGACCAGA AGGCCGTGGC GCTGGCGGCC GCCGCCCTGG AGCCCATGGA CGCCCTGACG 
GAGCAGCTGC GGAGGCTGGT GTCCTTGGAG CAGGATGACG GCGGCCAGGT GCTCCGCGCC 
GAGCGCGACG CCAGCCCGCT GCCCCGGGCG CTGGTCCGGA AGCTGTCGTC GCGCAGTCAG 
AGCAGGGTGC GCAACATCGC CAGCCGGGCC CGGGAGAAGC AGGAGGCCGG CAAGCAGAGG 
GGCGCCAAGC CCGGCCCCGC GGCGGGGGTG GTCCTCCGCC ACCGAGCCGC CCCGCCGCCC 
GCGGGGACCC GGCACTCCAC GGGCTCCTAC ATCGCGGGCT TCCTGCGCGG GGGCGCCGAG 
GACCGCGGCA TCCCCGAGGG CGCCTGCGCA GCGCTGCGCC CCGCCCCCGG AGACCACGCC 
CCCGGAGGCC CCGCCCCCGC CGCCCGCTTC TGCTCCCACG GCTCCGTCCT GCACACCGAG 
CCCGGCGGCG GCGGCGACGA CAAGCCCGAG ATCTATTTTC TCTTGAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634652.1
CDS	477..5510
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005634595.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634595.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CTGGGACTCA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTAGTCCGG 
CTCTTTTACC TGGATGAGCA TCGGACCCGC CTTCGATGGA GACCCTCGAG GAAGAGCGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTGACCG AGGGCCGGCA GTCTGAGATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAGG ACTCCCTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAGG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAACTG ATGCACAAGT TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TTTGTGTTTT TTACAAAATG ATGTCATTGA GACGGGACCT TTATTTGTTG 
CTCTTGAACT ACAGTGACAA GAAAGATCAC CTAACTCTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAG TAATGTGACA ACAGACTATT GTCTTGACAT CGTAAGAAAA 
TTTGAAGTTT CAGAAGAAAA TAAGGCAAAA AATGTTCTTG GCATAGAAGG GTTCACAAAT 
TTCATGCGTA GTCCTGCTTG TGACATATTT AACCCACTGC ACCATGAGGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACCGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA GGAGGGCTGT 
CGCTGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TACACTCTGA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCACGCC 
TTTGTGAAGA ACGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAACTGGA CCTGTCATCT 
GTAGACACCG GAGAGACCAA ACAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTATCATCTT GGGGATGATG CAGAGGAGGG GGAAGTTTCC 
GACGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTACAATAAT 
GGGACCACTG AGCATCAGGT GGAGTCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTGAAA 
GAATCTCAAA TCCGAGACAA GGAAGATCCC GATAGCTTCA CAGTGAGGGC GCTACTGAGG 
GCCACACACG AAGGCCTAAA TGCACACCTG AAGCAGCATC CAGATGTGAA GGAAAATGGG 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGCA GAAAACCACA 
AAATCACGAT CTAAGTCTTA CAGTACTGAC GATGAGGAGG ACACACAGCA GAATCCCGGC 
AAGGAAACCG GCCAGCTGTA CAGGTTGGGT CGGCGCAGGA AAACCATGAA GCTCTGCCGA 
GAGCTCTCTG ACTTGGTGGT GTATACAAAC TCCGTGGCCG CCCAGGACAT CGTGGATGAT 
GGAACCACAG GAAACGTGTT ATCATTTAGT GAAACAAGAG CACATCAGGT GGTTCAGCAG 
AAATCGGAGC AGTTCATGAT CTACAACCAA AAGCAGCTCA CGAGGATTTA CCCGTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCT CTGCCCTACT GGAACGCAGG CTGCCAGCTA 
GTGGCTCTGA ACTACCAGTC CGAAGGGCGA ATGATGCAAT TAAATCGAGC CAAATTCAAG 
ACCAATGGCA ATTGTGGCTA CGTCCTCAAA CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTACTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAGC AGCTCATCCT GAAAGTGATC 
AGTGGACAGC AGCTCCCCAA ACCTCCAGAC TCCATGTTCG GAGACCGAGG CGAGATCATT 
GATCCTTTCG TTGAAGTTGA AATTATTGGA TTGCCAGTAG ATTGCTGTAA AGATCAAACC 
CGTGTAGTAG ATGACAATGG ATTTAACCCT GTGTGGGAAG AAACCCTGAC GTTTACAGTA 
CACATGCCAG ACATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC GATTGGACGA 
GACTTCGTTG GACAAAGAAC TGTGACCTTC AGCAGCTTAG TGCCTGGCTA CCGGCACGTC 
TACTTGGAAG GACTGACAGA GGCATCGATA TTTGTCCACA TAACAATCAA TGAAATCTAT 
GGGAAGTGGA GCCCTTTAAT ACTCAACCCC AGTTATACTA TATTGCACTT TCTAGGAGCT 
ACAAAGAGCA GACAGCTCCA AGGTCTGAAG GGATTGTTCA ATAAGAATCC CAGGCATGGT 
TCTTCAGAAA ACAATTCCCA TTACGTTCGG AAACGATCAA TTGGAGATAG AATTCTACGC 
CGCACAGCCA GCGCTCCAGC CAAAGGCAGA AAGAAGAGCA AAATGGGCTT CCAAGAGATG 
GTAGAGATAA AGGATTCTGT ATCTGAGGCT GCGAGAGATC AGGATGGTGT CCTGAGGAGG 
ACCACACGGA GCTTGCAAGA GCGCCCTGTC TCTCTACCTG TTGACAAAAG TCTTCTGGGG 
GCCTTGTCAC TGCCCCTATC TGAAACAGCG AAAGACACTG AAGGAAAAGA AAACTCTCTG 
GCAGAAGATA AGGATGGAAG AAGAAAAGGG AAGGCAAGTA TAAAAGACCA ACACTTTCCA 
AATTTCAACA AAAAGTTATC CTCTTCCTCT AGCGCTCTCC TCCACAAAGA CCCCCGCCCA 
GGGGGCTCCG CTGTTTCTGT CGCCAGCGCA TCCAGCGCAT CCGTCACCGG GGAACAGCTG 
GGAGCCCCGG GCCTCCGGGG CGGGAGAACC AGATCACCTG CGCCAGGTGA CTGCCAGGAG 
CAGGAGTGTC CCCGCAAACC CCGCTCCCCA AGGCAGCATC TGGCTCTGGA ACCTGCACTT 
ACCGCAGCGC AAGGTGGTGT GAGCACCCAG GCACCTGCGC GCGCTGGAGG CCTTGGGGAG 
GACGGAAGCC CCCGGTCAGG AAGCAGGTTT TCTCAAAGCC ACCTGGAGGG CAGGGACTTG 
GAGGGCAGCT GGGTGACGGG CCGAGCCGCA GCGTCCCTCT CTCTGTCCGA CGTGTCTCTG 
CTGTGTGCTG CGCACCCCGA CTCGCACTCC GCGGCCGTTC TGCAGGAGAG CGACATCTCC 
CATCTTCTTG ACACTGTCCC TCTCGCTACC GAGGGCGACC CGGGCAGCTC CATCTCGGCG 
CTGGTCGGCC AGTGTGAGGA CGCCGGGGAG CCGGGGAGCC TCGCCGCGGC TCCTCCCCCG 
CACAGCACCT GCGTGGCGGC AGGTCATCCT CCCTTGCCCG CCCTGGACCC CGAGCCGGCC 
GTGGAGCCCG ACGCTGCCGC GGGAAAATGC AAGCCCTGCT TCGCGCGCGC ATCCCCCGCC 
CGGATTCCCG CCAAGGGCCC CGAGGCCTCG GCGCACACGC TTCACCAGAC CACCTGCGCC 
ACTGCCCCGC GGCCGCCCCC GGAGGACCAG CTCGCCGCGG GAAGCCACCG GCCCGGGGCC 
TCGGCCACGT GTCCCTGCCC GCCACCGCGA AGCCCCGGCG CCGGGCAGGT GCGAGGAGCG 
CCCGGGAGCC GCAGCCCCAC CCCCGCCCCG GCCCCCGCGG CCCCGCCAGC CGACCGCGCG 
CCCCGTTTCC ATCCTGGAGA CCGCAGTGTC GTGGGGGCGG GGGGCGCCGC AGGCCCCGCG 
GACCTGCCCG CACCTGCCGC TGAACGCCCC GGCGACGACG CAGGCCCCCT CGCGGCTCCT 
CTGCAGCCCA GGCGCAGCCC GCACGCGGTG GACCACGTCC CCGGAGCTCG GGGAAACGGC 
CGCTGCCCAG ACACCCCGCA CCCCCGCGTC CCCGCGGCGC CGCACAGCGC TCCCCAAAGC 
ACCTCTGGCC TCACCTGCCC AGGTGCTGCC GCTCCCACGC GCAGCCGCTG CTCGCACCCG 
GATGCCCACG CCGAGCAGGC CCGCGTGCCC GCGCCCCGCC ACGCGCCCCC GGGGCCCGCC 
CTCAAGCTGC CCAGCCCTTG CAAGTCCAAG AGCCTGGGCG ACCTGACATC CGAGGACATC 
GCCTGCAACT TCGAAAGCAA GTACCAGTGC ATCAGCAGGA GCTTTGTCGC CACCGGCCTC 
CGCGACCAGA AGGCCGTGGC GCTGGCGGCC GCCGCCCTGG AGCCCATGGA CGCCCTGACG 
GAGCAGCTGC GGAGGCTGGT GTCCTTGGAG CAGGATGACG GCGGCCAGGT GCTCCGCGCC 
GAGCGCGACG CCAGCCCGCT GCCCCGGGCG CTGGTCCGGA AGCTGTCGTC GCGCAGTCAG 
AGCAGGGTGC GCAACATCGC CAGCCGGGCC CGGGAGAAGC AGGAGGCCGG CAAGCAGAGG 
GGCGCCAAGC CCGGCCCCGC GGCGGGGGTG GTCCTCCGCC ACCGAGCCGC CCCGCCGCCC 
GCGGGGACCC GGCACTCCAC GGGCTCCTAC ATCGCGGGCT TCCTGCGCGG GGGCGCCGAG 
GACCGCGGCA TCCCCGAGGG CGCCTGCGCA GCGCTGCGCC CCGCCCCCGG AGACCACGCC 
CCCGGAGGCC CCGCCCCCGC CGCCCGCTTC TGCTCCCACG GCTCCGTCCT GCACACCGAG 
CCCGGCGGCG GCGGCGACGA CAAGCCCGAG ATCTATTTTC TCTTGAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005634595.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634652.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OCe24335
Clone ID Related Accession (Same CDS sequence)	XM_005634595.2 , XM_005634595.1
Accession Version	XM_005634595.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CTGGGACTCA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTAGTCCGG 
CTCTTTTACC TGGATGAGCA TCGGACCCGC CTTCGATGGA GACCCTCGAG GAAGAGCGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTGACCG AGGGCCGGCA GTCTGAGATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAGG ACTCCCTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAGG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAACTG ATGCACAAGT TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TTTGTGTTTT TTACAAAATG ATGTCATTGA GACGGGACCT TTATTTGTTG 
CTCTTGAACT ACAGTGACAA GAAAGATCAC CTAACTCTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAG TAATGTGACA ACAGACTATT GTCTTGACAT CGTAAGAAAA 
TTTGAAGTTT CAGAAGAAAA TAAGGCAAAA AATGTTCTTG GCATAGAAGG GTTCACAAAT 
TTCATGCGTA GTCCTGCTTG TGACATATTT AACCCACTGC ACCATGAGGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACCGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA GGAGGGCTGT 
CGCTGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TACACTCTGA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCACGCC 
TTTGTGAAGA ACGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAACTGGA CCTGTCATCT 
GTAGACACCG GAGAGACCAA ACAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTATCATCTT GGGGATGATG CAGAGGAGGG GGAAGTTTCC 
GACGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTACAATAAT 
GGGACCACTG AGCATCAGGT GGAGTCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTGAAA 
GAATCTCAAA TCCGAGACAA GGAAGATCCC GATAGCTTCA CAGTGAGGGC GCTACTGAGG 
GCCACACACG AAGGCCTAAA TGCACACCTG AAGCAGCATC CAGATGTGAA GGAAAATGGG 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGCA GAAAACCACA 
AAATCACGAT CTAAGTCTTA CAGTACTGAC GATGAGGAGG ACACACAGCA GAATCCCGGC 
AAGGAAACCG GCCAGCTGTA CAGGTTGGGT CGGCGCAGGA AAACCATGAA GCTCTGCCGA 
GAGCTCTCTG ACTTGGTGGT GTATACAAAC TCCGTGGCCG CCCAGGACAT CGTGGATGAT 
GGAACCACAG GAAACGTGTT ATCATTTAGT GAAACAAGAG CACATCAGGT GGTTCAGCAG 
AAATCGGAGC AGTTCATGAT CTACAACCAA AAGCAGCTCA CGAGGATTTA CCCGTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCT CTGCCCTACT GGAACGCAGG CTGCCAGCTA 
GTGGCTCTGA ACTACCAGTC CGAAGGGCGA ATGATGCAAT TAAATCGAGC CAAATTCAAG 
ACCAATGGCA ATTGTGGCTA CGTCCTCAAA CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTACTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAGC AGCTCATCCT GAAAGTGATC 
AGTGGACAGC AGCTCCCCAA ACCTCCAGAC TCCATGTTCG GAGACCGAGG CGAGATCATT 
GATCCTTTCG TTGAAGTTGA AATTATTGGA TTGCCAGTAG ATTGCTGTAA AGATCAAACC 
CGTGTAGTAG ATGACAATGG ATTTAACCCT GTGTGGGAAG AAACCCTGAC GTTTACAGTA 
CACATGCCAG ACATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC GATTGGACGA 
GACTTCGTTG GACAAAGAAC TGTGACCTTC AGCAGCTTAG TGCCTGGCTA CCGGCACGTC 
TACTTGGAAG GACTGACAGA GGCATCGATA TTTGTCCACA TAACAATCAA TGAAATCTAT 
GGGAAGTGGA GCCCTTTAAT ACTCAACCCC AGTTATACTA TATTGCACTT TCTAGGAGCT 
ACAAAGAGCA GACAGCTCCA AGGTCTGAAG GGATTGTTCA ATAAGAATCC CAGGCATGGT 
TCTTCAGAAA ACAATTCCCA TTACGTTCGG AAACGATCAA TTGGAGATAG AATTCTACGC 
CGCACAGCCA GCGCTCCAGC CAAAGGCAGA AAGAAGAGCA AAATGGGCTT CCAAGAGATG 
GTAGAGATAA AGGATTCTGT ATCTGAGGCT GCGAGAGATC AGGATGGTGT CCTGAGGAGG 
ACCACACGGA GCTTGCAAGA GCGCCCTGTC TCTCTACCTG TTGACAAAAG TCTTCTGGGG 
GCCTTGTCAC TGCCCCTATC TGAAACAGCG AAAGACACTG AAGGAAAAGA AAACTCTCTG 
GCAGAAGATA AGGATGGAAG AAGAAAAGGG AAGGCAAGTA TAAAAGACCA ACACTTTCCA 
AATTTCAACA AAAAGTTATC CTCTTCCTCT AGCGCTCTCC TCCACAAAGA CCCCCGCCCA 
GGGGGCTCCG CTGTTTCTGT CGCCAGCGCA TCCAGCGCAT CCGTCACCGG GGAACAGCTG 
GGAGCCCCGG GCCTCCGGGG CGGGAGAACC AGATCACCTG CGCCAGGTGA CTGCCAGGAG 
CAGGAGTGTC CCCGCAAACC CCGCTCCCCA AGGCAGCATC TGGCTCTGGA ACCTGCACTT 
ACCGCAGCGC AAGGTGGTGT GAGCACCCAG GCACCTGCGC GCGCTGGAGG CCTTGGGGAG 
GACGGAAGCC CCCGGTCAGG AAGCAGGTTT TCTCAAAGCC ACCTGGAGGG CAGGGACTTG 
GAGGGCAGCT GGGTGACGGG CCGAGCCGCA GCGTCCCTCT CTCTGTCCGA CGTGTCTCTG 
CTGTGTGCTG CGCACCCCGA CTCGCACTCC GCGGCCGTTC TGCAGGAGAG CGACATCTCC 
CATCTTCTTG ACACTGTCCC TCTCGCTACC GAGGGCGACC CGGGCAGCTC CATCTCGGCG 
CTGGTCGGCC AGTGTGAGGA CGCCGGGGAG CCGGGGAGCC TCGCCGCGGC TCCTCCCCCG 
CACAGCACCT GCGTGGCGGC AGGTCATCCT CCCTTGCCCG CCCTGGACCC CGAGCCGGCC 
GTGGAGCCCG ACGCTGCCGC GGGAAAATGC AAGCCCTGCT TCGCGCGCGC ATCCCCCGCC 
CGGATTCCCG CCAAGGGCCC CGAGGCCTCG GCGCACACGC TTCACCAGAC CACCTGCGCC 
ACTGCCCCGC GGCCGCCCCC GGAGGACCAG CTCGCCGCGG GAAGCCACCG GCCCGGGGCC 
TCGGCCACGT GTCCCTGCCC GCCACCGCGA AGCCCCGGCG CCGGGCAGGT GCGAGGAGCG 
CCCGGGAGCC GCAGCCCCAC CCCCGCCCCG GCCCCCGCGG CCCCGCCAGC CGACCGCGCG 
CCCCGTTTCC ATCCTGGAGA CCGCAGTGTC GTGGGGGCGG GGGGCGCCGC AGGCCCCGCG 
GACCTGCCCG CACCTGCCGC TGAACGCCCC GGCGACGACG CAGGCCCCCT CGCGGCTCCT 
CTGCAGCCCA GGCGCAGCCC GCACGCGGTG GACCACGTCC CCGGAGCTCG GGGAAACGGC 
CGCTGCCCAG ACACCCCGCA CCCCCGCGTC CCCGCGGCGC CGCACAGCGC TCCCCAAAGC 
ACCTCTGGCC TCACCTGCCC AGGTGCTGCC GCTCCCACGC GCAGCCGCTG CTCGCACCCG 
GATGCCCACG CCGAGCAGGC CCGCGTGCCC GCGCCCCGCC ACGCGCCCCC GGGGCCCGCC 
CTCAAGCTGC CCAGCCCTTG CAAGTCCAAG AGCCTGGGCG ACCTGACATC CGAGGACATC 
GCCTGCAACT TCGAAAGCAA GTACCAGTGC ATCAGCAGGA GCTTTGTCGC CACCGGCCTC 
CGCGACCAGA AGGCCGTGGC GCTGGCGGCC GCCGCCCTGG AGCCCATGGA CGCCCTGACG 
GAGCAGCTGC GGAGGCTGGT GTCCTTGGAG CAGGATGACG GCGGCCAGGT GCTCCGCGCC 
GAGCGCGACG CCAGCCCGCT GCCCCGGGCG CTGGTCCGGA AGCTGTCGTC GCGCAGTCAG 
AGCAGGGTGC GCAACATCGC CAGCCGGGCC CGGGAGAAGC AGGAGGCCGG CAAGCAGAGG 
GGCGCCAAGC CCGGCCCCGC GGCGGGGGTG GTCCTCCGCC ACCGAGCCGC CCCGCCGCCC 
GCGGGGACCC GGCACTCCAC GGGCTCCTAC ATCGCGGGCT TCCTGCGCGG GGGCGCCGAG 
GACCGCGGCA TCCCCGAGGG CGCCTGCGCA GCGCTGCGCC CCGCCCCCGG AGACCACGCC 
CCCGGAGGCC CCGCCCCCGC CGCCCGCTTC TGCTCCCACG GCTCCGTCCT GCACACCGAG 
CCCGGCGGCG GCGGCGACGA CAAGCCCGAG ATCTATTTTC TCTTGAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634652.1
CDS	440..5473
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005634595.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634595.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CTGGGACTCA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTAGTCCGG 
CTCTTTTACC TGGATGAGCA TCGGACCCGC CTTCGATGGA GACCCTCGAG GAAGAGCGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTGACCG AGGGCCGGCA GTCTGAGATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAGG ACTCCCTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT GAAGCAGACC TTTGAGGAGG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAACTG ATGCACAAGT TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TTTGTGTTTT TTACAAAATG ATGTCATTGA GACGGGACCT TTATTTGTTG 
CTCTTGAACT ACAGTGACAA GAAAGATCAC CTAACTCTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAG TAATGTGACA ACAGACTATT GTCTTGACAT CGTAAGAAAA 
TTTGAAGTTT CAGAAGAAAA TAAGGCAAAA AATGTTCTTG GCATAGAAGG GTTCACAAAT 
TTCATGCGTA GTCCTGCTTG TGACATATTT AACCCACTGC ACCATGAGGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACCGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA GGAGGGCTGT 
CGCTGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TACACTCTGA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCACGCC 
TTTGTGAAGA ACGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAACTGGA CCTGTCATCT 
GTAGACACCG GAGAGACCAA ACAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGCTGCC TTATCATCTT GGGGATGATG CAGAGGAGGG GGAAGTTTCC 
GACGAAGACA GCGCAGATGA AATTGAAGAT GAGTGCAAGT TCAAGCTCCA TTACAATAAT 
GGGACCACTG AGCATCAGGT GGAGTCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTGAAA 
GAATCTCAAA TCCGAGACAA GGAAGATCCC GATAGCTTCA CAGTGAGGGC GCTACTGAGG 
GCCACACACG AAGGCCTAAA TGCACACCTG AAGCAGCATC CAGATGTGAA GGAAAATGGG 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGCA GAAAACCACA 
AAATCACGAT CTAAGTCTTA CAGTACTGAC GATGAGGAGG ACACACAGCA GAATCCCGGC 
AAGGAAACCG GCCAGCTGTA CAGGTTGGGT CGGCGCAGGA AAACCATGAA GCTCTGCCGA 
GAGCTCTCTG ACTTGGTGGT GTATACAAAC TCCGTGGCCG CCCAGGACAT CGTGGATGAT 
GGAACCACAG GAAACGTGTT ATCATTTAGT GAAACAAGAG CACATCAGGT GGTTCAGCAG 
AAATCGGAGC AGTTCATGAT CTACAACCAA AAGCAGCTCA CGAGGATTTA CCCGTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCT CTGCCCTACT GGAACGCAGG CTGCCAGCTA 
GTGGCTCTGA ACTACCAGTC CGAAGGGCGA ATGATGCAAT TAAATCGAGC CAAATTCAAG 
ACCAATGGCA ATTGTGGCTA CGTCCTCAAA CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTACTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAGC AGCTCATCCT GAAAGTGATC 
AGTGGACAGC AGCTCCCCAA ACCTCCAGAC TCCATGTTCG GAGACCGAGG CGAGATCATT 
GATCCTTTCG TTGAAGTTGA AATTATTGGA TTGCCAGTAG ATTGCTGTAA AGATCAAACC 
CGTGTAGTAG ATGACAATGG ATTTAACCCT GTGTGGGAAG AAACCCTGAC GTTTACAGTA 
CACATGCCAG ACATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC GATTGGACGA 
GACTTCGTTG GACAAAGAAC TGTGACCTTC AGCAGCTTAG TGCCTGGCTA CCGGCACGTC 
TACTTGGAAG GACTGACAGA GGCATCGATA TTTGTCCACA TAACAATCAA TGAAATCTAT 
GGGAAGTGGA GCCCTTTAAT ACTCAACCCC AGTTATACTA TATTGCACTT TCTAGGAGCT 
ACAAAGAGCA GACAGCTCCA AGGTCTGAAG GGATTGTTCA ATAAGAATCC CAGGCATGGT 
TCTTCAGAAA ACAATTCCCA TTACGTTCGG AAACGATCAA TTGGAGATAG AATTCTACGC 
CGCACAGCCA GCGCTCCAGC CAAAGGCAGA AAGAAGAGCA AAATGGGCTT CCAAGAGATG 
GTAGAGATAA AGGATTCTGT ATCTGAGGCT GCGAGAGATC AGGATGGTGT CCTGAGGAGG 
ACCACACGGA GCTTGCAAGA GCGCCCTGTC TCTCTACCTG TTGACAAAAG TCTTCTGGGG 
GCCTTGTCAC TGCCCCTATC TGAAACAGCG AAAGACACTG AAGGAAAAGA AAACTCTCTG 
GCAGAAGATA AGGATGGAAG AAGAAAAGGG AAGGCAAGTA TAAAAGACCA ACACTTTCCA 
AATTTCAACA AAAAGTTATC CTCTTCCTCT AGCGCTCTCC TCCACAAAGA CCCCCGCCCA 
GGGGGCTCCG CTGTTTCTGT CGCCAGCGCA TCCAGCGCAT CCGTCACCGG GGAACAGCTG 
GGAGCCCCGG GCCTCCGGGG CGGGAGAACC AGATCACCTG CGCCAGGTGA CTGCCAGGAG 
CAGGAGTGTC CCCGCAAACC CCGCTCCCCA AGGCAGCATC TGGCTCTGGA ACCTGCACTT 
ACCGCAGCGC AAGGTGGTGT GAGCACCCAG GCACCTGCGC GCGCTGGAGG CCTTGGGGAG 
GACGGAAGCC CCCGGTCAGG AAGCAGGTTT TCTCAAAGCC ACCTGGAGGG CAGGGACTTG 
GAGGGCAGCT GGGTGACGGG CCGAGCCGCA GCGTCCCTCT CTCTGTCCGA CGTGTCTCTG 
CTGTGTGCTG CGCACCCCGA CTCGCACTCC GCGGCCGTTC TGCAGGAGAG CGACATCTCC 
CATCTTCTTG ACACTGTCCC TCTCGCTACC GAGGGCGACC CGGGCAGCTC CATCTCGGCG 
CTGGTCGGCC AGTGTGAGGA CGCCGGGGAG CCGGGGAGCC TCGCCGCGGC TCCTCCCCCG 
CACAGCACCT GCGTGGCGGC AGGTCATCCT CCCTTGCCCG CCCTGGACCC CGAGCCGGCC 
GTGGAGCCCG ACGCTGCCGC GGGAAAATGC AAGCCCTGCT TCGCGCGCGC ATCCCCCGCC 
CGGATTCCCG CCAAGGGCCC CGAGGCCTCG GCGCACACGC TTCACCAGAC CACCTGCGCC 
ACTGCCCCGC GGCCGCCCCC GGAGGACCAG CTCGCCGCGG GAAGCCACCG GCCCGGGGCC 
TCGGCCACGT GTCCCTGCCC GCCACCGCGA AGCCCCGGCG CCGGGCAGGT GCGAGGAGCG 
CCCGGGAGCC GCAGCCCCAC CCCCGCCCCG GCCCCCGCGG CCCCGCCAGC CGACCGCGCG 
CCCCGTTTCC ATCCTGGAGA CCGCAGTGTC GTGGGGGCGG GGGGCGCCGC AGGCCCCGCG 
GACCTGCCCG CACCTGCCGC TGAACGCCCC GGCGACGACG CAGGCCCCCT CGCGGCTCCT 
CTGCAGCCCA GGCGCAGCCC GCACGCGGTG GACCACGTCC CCGGAGCTCG GGGAAACGGC 
CGCTGCCCAG ACACCCCGCA CCCCCGCGTC CCCGCGGCGC CGCACAGCGC TCCCCAAAGC 
ACCTCTGGCC TCACCTGCCC AGGTGCTGCC GCTCCCACGC GCAGCCGCTG CTCGCACCCG 
GATGCCCACG CCGAGCAGGC CCGCGTGCCC GCGCCCCGCC ACGCGCCCCC GGGGCCCGCC 
CTCAAGCTGC CCAGCCCTTG CAAGTCCAAG AGCCTGGGCG ACCTGACATC CGAGGACATC 
GCCTGCAACT TCGAAAGCAA GTACCAGTGC ATCAGCAGGA GCTTTGTCGC CACCGGCCTC 
CGCGACCAGA AGGCCGTGGC GCTGGCGGCC GCCGCCCTGG AGCCCATGGA CGCCCTGACG 
GAGCAGCTGC GGAGGCTGGT GTCCTTGGAG CAGGATGACG GCGGCCAGGT GCTCCGCGCC 
GAGCGCGACG CCAGCCCGCT GCCCCGGGCG CTGGTCCGGA AGCTGTCGTC GCGCAGTCAG 
AGCAGGGTGC GCAACATCGC CAGCCGGGCC CGGGAGAAGC AGGAGGCCGG CAAGCAGAGG 
GGCGCCAAGC CCGGCCCCGC GGCGGGGGTG GTCCTCCGCC ACCGAGCCGC CCCGCCGCCC 
GCGGGGACCC GGCACTCCAC GGGCTCCTAC ATCGCGGGCT TCCTGCGCGG GGGCGCCGAG 
GACCGCGGCA TCCCCGAGGG CGCCTGCGCA GCGCTGCGCC CCGCCCCCGG AGACCACGCC 
CCCGGAGGCC CCGCCCCCGC CGCCCGCTTC TGCTCCCACG GCTCCGTCCT GCACACCGAG 
CCCGGCGGCG GCGGCGACGA CAAGCCCGAG ATCTATTTTC TCTTGAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106731.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962206.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe24336
Clone ID Related Accession (Same CDS sequence)	XM_014106731.1 , XM_022408776.1
Accession Version	XM_014106731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962206.1
CDS	248..5254
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106731.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106731.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_022408776.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264484.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe24336
Clone ID Related Accession (Same CDS sequence)	XM_014106731.1 , XM_022408776.1
Accession Version	XM_022408776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264484.1
CDS	1..5007
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_022408776.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408776.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106734.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962209.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X11, mRNA.

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CloneID	OCe24338
Clone ID Related Accession (Same CDS sequence)	XM_014106734.1 , XM_014106733.1 , XM_005634601.2 , XM_014106732.1
Accession Version	XM_014106734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962209.1
CDS	32..5011
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106734.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106734.1

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106733.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962208.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X10, mRNA.

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CloneID	OCe24338
Clone ID Related Accession (Same CDS sequence)	XM_014106734.1 , XM_014106733.1 , XM_005634601.2 , XM_014106732.1
Accession Version	XM_014106733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962208.1
CDS	85..5064
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106733.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106733.1

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005634601.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634658.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X12, mRNA.

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CloneID	OCe24338
Clone ID Related Accession (Same CDS sequence)	XM_014106734.1 , XM_014106733.1 , XM_005634601.2 , XM_014106732.1
Accession Version	XM_005634601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634658.1
CDS	97..5076
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005634601.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634601.2

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106732.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962207.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OCe24338
Clone ID Related Accession (Same CDS sequence)	XM_014106734.1 , XM_014106733.1 , XM_005634601.2 , XM_014106732.1
Accession Version	XM_014106732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962207.1
CDS	82..5061
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106732.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106732.1

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCTGG GACTCAGATG ATCAAACTGA AACGTGGAAC CAAAGGGCTA 
GTCCGGCTCT TTTACCTGGA TGAGCATCGG ACCCGCCTTC GATGGAGACC CTCGAGGAAG 
AGCGAGAAGG CAAAAATACT TATTGACTCC ATTTACAAAG TGACCGAGGG CCGGCAGTCT 
GAGATATTCC ACAGACAAGC TGAGGGGCAC TTCGATCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC TCTGGATCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAGGACTC CCTTGCCAAG 
CGGCAGAGGA CCCACGACCA GTGGGTGAAG CAGACCTTTG AGGAGGCTGA TAAGAACGGT 
GATGGCTTAT TGAATATTGA AGAGATACAC CAACTGATGC ACAAGTTAAA TGTCAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCAGATA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAATTTTG TGTTTTTTAC AAAATGATGT CATTGAGACG GGACCTTTAT 
TTGTTGCTCT TGAACTACAG TGACAAGAAA GATCACCTAA CTCTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAGTAAT GTGACAACAG ACTATTGTCT TGACATCGTA 
AGAAAATTTG AAGTTTCAGA AGAAAATAAG GCAAAAAATG TTCTTGGCAT AGAAGGGTTC 
ACAAATTTCA TGCGTAGTCC TGCTTGTGAC ATATTTAACC CACTGCACCA TGAGGTGTAC 
CAGGACATGG ATCAGCCTCT GTGCAACTAC TACATCGCTT CCTCTCACAA CACATACCTG 
ACCGGGGACC AGCTCCTTTC CCAGTCCAAA GTGGACATGT ATGCACGGGT GCTACAGGAG 
GGCTGTCGCT GCGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTGGTCCAC 
CATGGTTACA CTCTGACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CACGCCTTTG TGAAGAACGA GTTCCCGGTC ATACTGTCCA TTGAGAATCA CTGCAGTGTC 
CAGCAGCAGA GGAAGATTGC TCAGTACCTG AAAGGAATAT TTGGAGACAA ACTGGACCTG 
TCATCTGTAG ACACCGGAGA GACCAAACAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT CATCTTGGGG ATGATGCAGA GGAGGGGGAA 
GTTTCCGACG AAGACAGCGC AGATGAAATT GAAGATGAGT GCAAGTTCAA GCTCCATTAC 
AATAATGGGA CCACTGAGCA TCAGGTGGAG TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTGAAAGAAT CTCAAATCCG AGACAAGGAA GATCCCGATA GCTTCACAGT GAGGGCGCTA 
CTGAGGGCCA CACACGAAGG CCTAAATGCA CACCTGAAGC AGCATCCAGA TGTGAAGGAA 
AATGGGAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGCAGAAA 
ACCACAAAAT CACGATCTAA GTCTTACAGT ACTGACGATG AGGAGGACAC ACAGCAGAAT 
CCCGGCAAGG AAACCGGCCA GCTGTACAGG TTGGGTCGGC GCAGGAAAAC CATGAAGCTC 
TGCCGAGAGC TCTCTGACTT GGTGGTGTAT ACAAACTCCG TGGCCGCCCA GGACATCGTG 
GATGATGGAA CCACAGGAAA CGTGTTATCA TTTAGTGAAA CAAGAGCACA TCAGGTGGTT 
CAGCAGAAAT CGGAGCAGTT CATGATCTAC AACCAAAAGC AGCTCACGAG GATTTACCCG 
TCTGCCTACC GCATCGATTC CAGTAACTTC AACCCTCTGC CCTACTGGAA CGCAGGCTGC 
CAGCTAGTGG CTCTGAACTA CCAGTCCGAA GGGCGAATGA TGCAATTAAA TCGAGCCAAA 
TTCAAGACCA ATGGCAATTG TGGCTACGTC CTCAAACCCC AGCAAATGTG CAAAGGTACT 
TTCAACCCTT ACTCTGGTGA CCCACTTCCT GCCAACCCCA AAAAGCAGCT CATCCTGAAA 
GTGATCAGTG GACAGCAGCT CCCCAAACCT CCAGACTCCA TGTTCGGAGA CCGAGGCGAG 
ATCATTGATC CTTTCGTTGA AGTTGAAATT ATTGGATTGC CAGTAGATTG CTGTAAAGAT 
CAAACCCGTG TAGTAGATGA CAATGGATTT AACCCTGTGT GGGAAGAAAC CCTGACGTTT 
ACAGTACACA TGCCAGACAT AGCTTTGGTT CGGTTCCTTG TGTGGGATCA TGATCCGATT 
GGACGAGACT TCGTTGGACA AAGAACTGTG ACCTTCAGCA GCTTAGTGCC TGGCTACCGG 
CACGTCTACT TGGAAGGACT GACAGAGGCA TCGATATTTG TCCACATAAC AATCAATGAA 
ATCTATGGGA AGTGGAGCCC TTTAATACTC AACCCCAGTT ATACTATATT GCACTTTCTA 
GGAGCTACAA AGAGCAGACA GCTCCAAGGT CTGAAGGGAT TGTTCAATAA GAATCCCAGG 
CATGGTTCTT CAGAAAACAA TTCCCATTAC GTTCGGAAAC GATCAATTGG AGATAGAATT 
CTACGCCGCA CAGCCAGCGC TCCAGCCAAA GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA 
GAGATGGTAG AGATAAAGGA TTCTGTATCT GAGGCTGCGA GAGATCAGGA TGGTGTCCTG 
AGGAGGACCA CACGGAGCTT GCAAGAGCGC CCTGTCTCTC TACCTGTTGA CAAAAGTCTT 
CTGGGGGCCT TGTCACTGCC CCTATCTGAA ACAGCGAAAG ACACTGAAGG AAAAGAAAAC 
TCTCTGGCAG AAGATAAGGA TGGAAGAAGA AAAGGGAAGG CAAGTATAAA AGACCAACAC 
TTTCCAAATT TCAACAAAAA GTTATCCTCT TCCTCTAGCG CTCTCCTCCA CAAAGACCCC 
CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC AGCGCATCCA GCGCATCCGT CACCGGGGAA 
CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG AGAACCAGAT CACCTGCGCC AGGTGACTGC 
CAGGAGCAGG AGTGTCCCCG CAAACCCCGC TCCCCAAGGC AGCATCTGGC TCTGGAACCT 
GCACTTACCG CAGCGCAAGG TGGTGTGAGC ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT 
GGGGAGGACG GAAGCCCCCG GTCAGGAAGC AGGTTTTCTC AAAGCCACCT GGAGGGCAGG 
GACTTGGAGG GCAGCTGGGT GACGGGCCGA GCCGCAGCGT CCCTCTCTCT GTCCGACGTG 
TCTCTGCTGT GTGCTGCGCA CCCCGACTCG CACTCCGCGG CCGTTCTGCA GGAGAGCGAC 
ATCTCCCATC TTCTTGACAC TGTCCCTCTC GCTACCGAGG GCGACCCGGG CAGCTCCATC 
TCGGCGCTGG TCGGCCAGTG TGAGGACGCC GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT 
CCCCCGCACA GCACCTGCGT GGCGGCAGGT CATCCTCCCT TGCCCGCCCT GGACCCCGAG 
CCGGCCGTGG AGCCCGACGC TGCCGCGGGA AAATGCAAGC CCTGCTTCGC GCGCGCATCC 
CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG GCCTCGGCGC ACACGCTTCA CCAGACCACC 
TGCGCCACTG CCCCGCGGCC GCCCCCGGAG GACCAGCTCG CCGCGGGAAG CCACCGGCCC 
GGGGCCTCGG CCACGTGTCC CTGCCCGCCA CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA 
GGAGCGCCCG GGAGCCGCAG CCCCACCCCC GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC 
CGCGCGCCCC GTTTCCATCC TGGAGACCGC AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC 
CCCGCGGACC TGCCCGCACC TGCCGCTGAA CGCCCCGGCG ACGACGCAGG CCCCCTCGCG 
GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC GCGGTGGACC ACGTCCCCGG AGCTCGGGGA 
AACGGCCGCT GCCCAGACAC CCCGCACCCC CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC 
CAAAGCACCT CTGGCCTCAC CTGCCCAGGT GCTGCCGCTC CCACGCGCAG CCGCTGCTCG 
CACCCGGATG CCCACGCCGA GCAGGCCCGC GTGCCCGCGC CCCGCCACGC GCCCCCGGGG 
CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG TCCAAGAGCC TGGGCGACCT GACATCCGAG 
GACATCGCCT GCAACTTCGA AAGCAAGTAC CAGTGCATCA GCAGGAGCTT TGTCGCCACC 
GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG GCGGCCGCCG CCCTGGAGCC CATGGACGCC 
CTGACGGAGC AGCTGCGGAG GCTGGTGTCC TTGGAGCAGG ATGACGGCGG CCAGGTGCTC 
CGCGCCGAGC GCGACGCCAG CCCGCTGCCC CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC 
AGTCAGAGCA GGGTGCGCAA CATCGCCAGC CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG 
CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG GGGGTGGTCC TCCGCCACCG AGCCGCCCCG 
CCGCCCGCGG GGACCCGGCA CTCCACGGGC TCCTACATCG CGGGCTTCCT GCGCGGGGGC 
GCCGAGGACC GCGGCATCCC CGAGGGCGCC TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC 
CACGCCCCCG GAGGCCCCGC CCCCGCCGCC CGCTTCTGCT CCCACGGCTC CGTCCTGCAC 
ACCGAGCCCG GCGGCGGCGG CGACGACAAG CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_022408777.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264485.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe296576
Clone ID Related Accession (Same CDS sequence)	XM_022408777.1
Accession Version	XM_022408777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGAAGATA AGGATGGAAG AAGAAAAGGG 
AAGGCAAGTA TAAAAGACCA ACACTTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCT 
AGCGCTCTCC TCCACAAAGA CCCCCGCCCA GGGGGCTCCG CTGTTTCTGT CGCCAGCGCA 
TCCAGCGCAT CCGTCACCGG GGAACAGCTG GGAGCCCCGG GCCTCCGGGG CGGGAGAACC 
AGATCACCTG CGCCAGGTGA CTGCCAGGAG CAGGAGTGTC CCCGCAAACC CCGCTCCCCA 
AGGCAGCATC TGGCTCTGGA ACCTGCACTT ACCGCAGCGC AAGGTGGTGT GAGCACCCAG 
GCACCTGCGC GCGCTGGAGG CCTTGGGGAG GACGGAAGCC CCCGGTCAGG AAGCAGGTTT 
TCTCAAAGCC ACCTGGAGGG CAGGGACTTG GAGGGCAGCT GGGTGACGGG CCGAGCCGCA 
GCGTCCCTCT CTCTGTCCGA CGTGTCTCTG CTGTGTGCTG CGCACCCCGA CTCGCACTCC 
GCGGCCGTTC TGCAGGAGAG CGACATCTCC CATCTTCTTG ACACTGTCCC TCTCGCTACC 
GAGGGCGACC CGGGCAGCTC CATCTCGGCG CTGGTCGGCC AGTGTGAGGA CGCCGGGGAG 
CCGGGGAGCC TCGCCGCGGC TCCTCCCCCG CACAGCACCT GCGTGGCGGC AGGTCATCCT 
CCCTTGCCCG CCCTGGACCC CGAGCCGGCC GTGGAGCCCG ACGCTGCCGC GGGAAAATGC 
AAGCCCTGCT TCGCGCGCGC ATCCCCCGCC CGGATTCCCG CCAAGGGCCC CGAGGCCTCG 
GCGCACACGC TTCACCAGAC CACCTGCGCC ACTGCCCCGC GGCCGCCCCC GGAGGACCAG 
CTCGCCGCGG GAAGCCACCG GCCCGGGGCC TCGGCCACGT GTCCCTGCCC GCCACCGCGA 
AGCCCCGGCG CCGGGCAGGT GCGAGGAGCG CCCGGGAGCC GCAGCCCCAC CCCCGCCCCG 
GCCCCCGCGG CCCCGCCAGC CGACCGCGCG CCCCGTTTCC ATCCTGGAGA CCGCAGTGTC 
GTGGGGGCGG GGGGCGCCGC AGGCCCCGCG GACCTGCCCG CACCTGCCGC TGAACGCCCC 
GGCGACGACG CAGGCCCCCT CGCGGCTCCT CTGCAGCCCA GGCGCAGCCC GCACGCGGTG 
GACCACGTCC CCGGAGCTCG GGGAAACGGC CGCTGCCCAG ACACCCCGCA CCCCCGCGTC 
CCCGCGGCGC CGCACAGCGC TCCCCAAAGC ACCTCTGGCC TCACCTGCCC AGGTGCTGCC 
GCTCCCACGC GCAGCCGCTG CTCGCACCCG GATGCCCACG CCGAGCAGGC CCGCGTGCCC 
GCGCCCCGCC ACGCGCCCCC GGGGCCCGCC CTCAAGCTGC CCAGCCCTTG CAAGTCCAAG 
AGCCTGGGCG ACCTGACATC CGAGGACATC GCCTGCAACT TCGAAAGCAA GTACCAGTGC 
ATCAGCAGGA GCTTTGTCGC CACCGGCCTC CGCGACCAGA AGGCCGTGGC GCTGGCGGCC 
GCCGCCCTGG AGCCCATGGA CGCCCTGACG GAGCAGCTGC GGAGGCTGGT GTCCTTGGAG 
CAGGATGACG GCGGCCAGGT GCTCCGCGCC GAGCGCGACG CCAGCCCGCT GCCCCGGGCG 
CTGGTCCGGA AGCTGTCGTC GCGCAGTCAG AGCAGGGTGC GCAACATCGC CAGCCGGGCC 
CGGGAGAAGC AGGAGGCCGG CAAGCAGAGG GGCGCCAAGC CCGGCCCCGC GGCGGGGGTG 
GTCCTCCGCC ACCGAGCCGC CCCGCCGCCC GCGGGGACCC GGCACTCCAC GGGCTCCTAC 
ATCGCGGGCT TCCTGCGCGG GGGCGCCGAG GACCGCGGCA TCCCCGAGGG CGCCTGCGCA 
GCGCTGCGCC CCGCCCCCGG AGACCACGCC CCCGGAGGCC CCGCCCCCGC CGCCCGCTTC 
TGCTCCCACG GCTCCGTCCT GCACACCGAG CCCGGCGGCG GCGGCGACGA CAAGCCCGAG 
ATCTATTTTC TCTTGAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264485.1
CDS	1..5004
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_022408777.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408777.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGAAGATA AGGATGGAAG AAGAAAAGGG 
AAGGCAAGTA TAAAAGACCA ACACTTTCCA AATTTCAACA AAAAGTTATC CTCTTCCTCT 
AGCGCTCTCC TCCACAAAGA CCCCCGCCCA GGGGGCTCCG CTGTTTCTGT CGCCAGCGCA 
TCCAGCGCAT CCGTCACCGG GGAACAGCTG GGAGCCCCGG GCCTCCGGGG CGGGAGAACC 
AGATCACCTG CGCCAGGTGA CTGCCAGGAG CAGGAGTGTC CCCGCAAACC CCGCTCCCCA 
AGGCAGCATC TGGCTCTGGA ACCTGCACTT ACCGCAGCGC AAGGTGGTGT GAGCACCCAG 
GCACCTGCGC GCGCTGGAGG CCTTGGGGAG GACGGAAGCC CCCGGTCAGG AAGCAGGTTT 
TCTCAAAGCC ACCTGGAGGG CAGGGACTTG GAGGGCAGCT GGGTGACGGG CCGAGCCGCA 
GCGTCCCTCT CTCTGTCCGA CGTGTCTCTG CTGTGTGCTG CGCACCCCGA CTCGCACTCC 
GCGGCCGTTC TGCAGGAGAG CGACATCTCC CATCTTCTTG ACACTGTCCC TCTCGCTACC 
GAGGGCGACC CGGGCAGCTC CATCTCGGCG CTGGTCGGCC AGTGTGAGGA CGCCGGGGAG 
CCGGGGAGCC TCGCCGCGGC TCCTCCCCCG CACAGCACCT GCGTGGCGGC AGGTCATCCT 
CCCTTGCCCG CCCTGGACCC CGAGCCGGCC GTGGAGCCCG ACGCTGCCGC GGGAAAATGC 
AAGCCCTGCT TCGCGCGCGC ATCCCCCGCC CGGATTCCCG CCAAGGGCCC CGAGGCCTCG 
GCGCACACGC TTCACCAGAC CACCTGCGCC ACTGCCCCGC GGCCGCCCCC GGAGGACCAG 
CTCGCCGCGG GAAGCCACCG GCCCGGGGCC TCGGCCACGT GTCCCTGCCC GCCACCGCGA 
AGCCCCGGCG CCGGGCAGGT GCGAGGAGCG CCCGGGAGCC GCAGCCCCAC CCCCGCCCCG 
GCCCCCGCGG CCCCGCCAGC CGACCGCGCG CCCCGTTTCC ATCCTGGAGA CCGCAGTGTC 
GTGGGGGCGG GGGGCGCCGC AGGCCCCGCG GACCTGCCCG CACCTGCCGC TGAACGCCCC 
GGCGACGACG CAGGCCCCCT CGCGGCTCCT CTGCAGCCCA GGCGCAGCCC GCACGCGGTG 
GACCACGTCC CCGGAGCTCG GGGAAACGGC CGCTGCCCAG ACACCCCGCA CCCCCGCGTC 
CCCGCGGCGC CGCACAGCGC TCCCCAAAGC ACCTCTGGCC TCACCTGCCC AGGTGCTGCC 
GCTCCCACGC GCAGCCGCTG CTCGCACCCG GATGCCCACG CCGAGCAGGC CCGCGTGCCC 
GCGCCCCGCC ACGCGCCCCC GGGGCCCGCC CTCAAGCTGC CCAGCCCTTG CAAGTCCAAG 
AGCCTGGGCG ACCTGACATC CGAGGACATC GCCTGCAACT TCGAAAGCAA GTACCAGTGC 
ATCAGCAGGA GCTTTGTCGC CACCGGCCTC CGCGACCAGA AGGCCGTGGC GCTGGCGGCC 
GCCGCCCTGG AGCCCATGGA CGCCCTGACG GAGCAGCTGC GGAGGCTGGT GTCCTTGGAG 
CAGGATGACG GCGGCCAGGT GCTCCGCGCC GAGCGCGACG CCAGCCCGCT GCCCCGGGCG 
CTGGTCCGGA AGCTGTCGTC GCGCAGTCAG AGCAGGGTGC GCAACATCGC CAGCCGGGCC 
CGGGAGAAGC AGGAGGCCGG CAAGCAGAGG GGCGCCAAGC CCGGCCCCGC GGCGGGGGTG 
GTCCTCCGCC ACCGAGCCGC CCCGCCGCCC GCGGGGACCC GGCACTCCAC GGGCTCCTAC 
ATCGCGGGCT TCCTGCGCGG GGGCGCCGAG GACCGCGGCA TCCCCGAGGG CGCCTGCGCA 
GCGCTGCGCC CCGCCCCCGG AGACCACGCC CCCGGAGGCC CCGCCCCCGC CGCCCGCTTC 
TGCTCCCACG GCTCCGTCCT GCACACCGAG CCCGGCGGCG GCGGCGACGA CAAGCCCGAG 
ATCTATTTTC TCTTGAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106727.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962202.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe42578
Clone ID Related Accession (Same CDS sequence)	XM_014106727.1 , XM_014106727.2
Accession Version	XM_014106727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962202.1
CDS	248..5314
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106727.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106727.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106727.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962202.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OCe42578
Clone ID Related Accession (Same CDS sequence)	XM_014106727.1 , XM_014106727.2
Accession Version	XM_014106727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014106727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962202.1
CDS	199..5265
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106727.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106727.2

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGTGGAGCCC TTTAATACTC 
AACCCCAGTT ATACTATATT GCACTTTCTA GGAGCTACAA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_022408775.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264483.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OCe42579
Clone ID Related Accession (Same CDS sequence)	XM_022408775.1 , XM_014106728.1
Accession Version	XM_022408775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264483.1
CDS	1..5067
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_022408775.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408775.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106728.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962203.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OCe42579
Clone ID Related Accession (Same CDS sequence)	XM_022408775.1 , XM_014106728.1
Accession Version	XM_014106728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962203.1
CDS	248..5314
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106728.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106728.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GATCTAAGTC TTACAGTACT 
GACGATGAGG AGGACACACA GCAGAATCCC GGCAAGGAAA CCGGCCAGCT GTACAGGTTG 
GGTCGGCGCA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTGTATACA 
AACTCCGTGG CCGCCCAGGA CATCGTGGAT GATGGAACCA CAGGAAACGT GTTATCATTT 
AGTGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATCTACAAC 
CAAAAGCAGC TCACGAGGAT TTACCCGTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCTCTGCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCTC TGAACTACCA GTCCGAAGGG 
CGAATGATGC AATTAAATCG AGCCAAATTC AAGACCAATG GCAATTGTGG CTACGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTACT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGTGGAC AGCAGCTCCC CAAACCTCCA 
GACTCCATGT TCGGAGACCG AGGCGAGATC ATTGATCCTT TCGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGCTG TAAAGATCAA ACCCGTGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACGTTTACA GTACACATGC CAGACATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTCG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAC GTCTACTTGG AAGGACTGAC AGAGGCATCG 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGGAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA GCAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAT GGTTCTTCAG AAAACAATTC CCATTACGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTA CGCCGCACAG CCAGCGCTCC AGCCAAAGGC 
AGAAAGAAGA GCAAAATGGG CTTCCAAGAG ATGGTAGAGA TAAAGGATTC TGTATCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGCTTGCA AGAGCGCCCT 
GTCTCTCTAC CTGTTGACAA AAGTCTTCTG GGGGCCTTGT CACTGCCCCT ATCTGAAACA 
GCGAAAGACA CTGAAGGAAA AGAAAACTCT CTGGCAGAAG ATAAGGATGG AAGAAGAAAA 
GGGAAGGCAA GTATAAAAGA CCAACACTTT CCAAATTTCA ACAAAAAGTT ATCCTCTTCC 
TCTAGCGCTC TCCTCCACAA AGACCCCCGC CCAGGGGGCT CCGCTGTTTC TGTCGCCAGC 
GCATCCAGCG CATCCGTCAC CGGGGAACAG CTGGGAGCCC CGGGCCTCCG GGGCGGGAGA 
ACCAGATCAC CTGCGCCAGG TGACTGCCAG GAGCAGGAGT GTCCCCGCAA ACCCCGCTCC 
CCAAGGCAGC ATCTGGCTCT GGAACCTGCA CTTACCGCAG CGCAAGGTGG TGTGAGCACC 
CAGGCACCTG CGCGCGCTGG AGGCCTTGGG GAGGACGGAA GCCCCCGGTC AGGAAGCAGG 
TTTTCTCAAA GCCACCTGGA GGGCAGGGAC TTGGAGGGCA GCTGGGTGAC GGGCCGAGCC 
GCAGCGTCCC TCTCTCTGTC CGACGTGTCT CTGCTGTGTG CTGCGCACCC CGACTCGCAC 
TCCGCGGCCG TTCTGCAGGA GAGCGACATC TCCCATCTTC TTGACACTGT CCCTCTCGCT 
ACCGAGGGCG ACCCGGGCAG CTCCATCTCG GCGCTGGTCG GCCAGTGTGA GGACGCCGGG 
GAGCCGGGGA GCCTCGCCGC GGCTCCTCCC CCGCACAGCA CCTGCGTGGC GGCAGGTCAT 
CCTCCCTTGC CCGCCCTGGA CCCCGAGCCG GCCGTGGAGC CCGACGCTGC CGCGGGAAAA 
TGCAAGCCCT GCTTCGCGCG CGCATCCCCC GCCCGGATTC CCGCCAAGGG CCCCGAGGCC 
TCGGCGCACA CGCTTCACCA GACCACCTGC GCCACTGCCC CGCGGCCGCC CCCGGAGGAC 
CAGCTCGCCG CGGGAAGCCA CCGGCCCGGG GCCTCGGCCA CGTGTCCCTG CCCGCCACCG 
CGAAGCCCCG GCGCCGGGCA GGTGCGAGGA GCGCCCGGGA GCCGCAGCCC CACCCCCGCC 
CCGGCCCCCG CGGCCCCGCC AGCCGACCGC GCGCCCCGTT TCCATCCTGG AGACCGCAGT 
GTCGTGGGGG CGGGGGGCGC CGCAGGCCCC GCGGACCTGC CCGCACCTGC CGCTGAACGC 
CCCGGCGACG ACGCAGGCCC CCTCGCGGCT CCTCTGCAGC CCAGGCGCAG CCCGCACGCG 
GTGGACCACG TCCCCGGAGC TCGGGGAAAC GGCCGCTGCC CAGACACCCC GCACCCCCGC 
GTCCCCGCGG CGCCGCACAG CGCTCCCCAA AGCACCTCTG GCCTCACCTG CCCAGGTGCT 
GCCGCTCCCA CGCGCAGCCG CTGCTCGCAC CCGGATGCCC ACGCCGAGCA GGCCCGCGTG 
CCCGCGCCCC GCCACGCGCC CCCGGGGCCC GCCCTCAAGC TGCCCAGCCC TTGCAAGTCC 
AAGAGCCTGG GCGACCTGAC ATCCGAGGAC ATCGCCTGCA ACTTCGAAAG CAAGTACCAG 
TGCATCAGCA GGAGCTTTGT CGCCACCGGC CTCCGCGACC AGAAGGCCGT GGCGCTGGCG 
GCCGCCGCCC TGGAGCCCAT GGACGCCCTG ACGGAGCAGC TGCGGAGGCT GGTGTCCTTG 
GAGCAGGATG ACGGCGGCCA GGTGCTCCGC GCCGAGCGCG ACGCCAGCCC GCTGCCCCGG 
GCGCTGGTCC GGAAGCTGTC GTCGCGCAGT CAGAGCAGGG TGCGCAACAT CGCCAGCCGG 
GCCCGGGAGA AGCAGGAGGC CGGCAAGCAG AGGGGCGCCA AGCCCGGCCC CGCGGCGGGG 
GTGGTCCTCC GCCACCGAGC CGCCCCGCCG CCCGCGGGGA CCCGGCACTC CACGGGCTCC 
TACATCGCGG GCTTCCTGCG CGGGGGCGCC GAGGACCGCG GCATCCCCGA GGGCGCCTGC 
GCAGCGCTGC GCCCCGCCCC CGGAGACCAC GCCCCCGGAG GCCCCGCCCC CGCCGCCCGC 
TTCTGCTCCC ACGGCTCCGT CCTGCACACC GAGCCCGGCG GCGGCGGCGA CGACAAGCCC 
GAGATCTATT TTCTCTTGAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106729.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962204.1 Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OCe42580
Clone ID Related Accession (Same CDS sequence)	XM_014106729.2 , XM_014106729.1
Accession Version	XM_014106729.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014106729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962204.1
CDS	198..5207
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106729.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106729.2

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_014106729.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962204.1 Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OCe42580
Clone ID Related Accession (Same CDS sequence)	XM_014106729.2 , XM_014106729.1
Accession Version	XM_014106729.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962204.1
CDS	248..5257
Translation	MSYWDAEGRSRVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLNYSDKKDHLTLEELAQFLKVEQKMSNVTTDYCLDIVRKFEVSEENKAKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLRATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKQKTTKSRSKSYSTDDEEDTQQNPGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPYSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPDIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKSRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSLPVDKSLLGALSLPLSETAKDTEGKENSLAEDKDGRRKGKASIKDQHFPNFNKKLSSSSSALLHKDPRPGGSAVSVASASSASVTGEQLGAPGLRGGRTRSPAPGDCQEQECPRKPRSPRQHLALEPALTAAQGGVSTQAPARAGGLGEDGSPRSGSRFSQSHLEGRDLEGSWVTGRAAASLSLSDVSLLCAAHPDSHSAAVLQESDISHLLDTVPLATEGDPGSSISALVGQCEDAGEPGSLAAAPPPHSTCVAAGHPPLPALDPEPAVEPDAAAGKCKPCFARASPARIPAKGPEASAHTLHQTTCATAPRPPPEDQLAAGSHRPGASATCPCPPPRSPGAGQVRGAPGSRSPTPAPAPAAPPADRAPRFHPGDRSVVGAGGAAGPADLPAPAAERPGDDAGPLAAPLQPRRSPHAVDHVPGARGNGRCPDTPHPRVPAAPHSAPQSTSGLTCPGAAAPTRSRCSHPDAHAEQARVPAPRHAPPGPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVATGLRDQKAVALAAAALEPMDALTEQLRRLVSLEQDDGGQVLRAERDASPLPRALVRKLSSRSQSRVRNIASRAREKQEAGKQRGAKPGPAAGVVLRHRAAPPPAGTRHSTGSYIAGFLRGGAEDRGIPEGACAALRPAPGDHAPGGPAPAARFCSHGSVLHTEPGGGGDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_014106729.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106729.1

ORF Insert Sequence:

ATGAGCTACT GGGACGCGGA GGGAAGGAGC CGTGTGCAGT ACCGGCGGCA TTTCCTGGTG 
GACAACAGCG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCTGG GACTCAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTA GTCCGGCTCT TTTACCTGGA TGAGCATCGG 
ACCCGCCTTC GATGGAGACC CTCGAGGAAG AGCGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TGACCGAGGG CCGGCAGTCT GAGATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAGGACTC CCTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTGAAG 
CAGACCTTTG AGGAGGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAACTGATGC ACAAGTTAAA TGTCAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTTTG TGTTTTTTAC 
AAAATGATGT CATTGAGACG GGACCTTTAT TTGTTGCTCT TGAACTACAG TGACAAGAAA 
GATCACCTAA CTCTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAGTAAT 
GTGACAACAG ACTATTGTCT TGACATCGTA AGAAAATTTG AAGTTTCAGA AGAAAATAAG 
GCAAAAAATG TTCTTGGCAT AGAAGGGTTC ACAAATTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAACC CACTGCACCA TGAGGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACCGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAGGAG GGCTGTCGCT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTACA CTCTGACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CACGCCTTTG TGAAGAACGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA ACTGGACCTG TCATCTGTAG ACACCGGAGA GACCAAACAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GCTGCCTTAT 
CATCTTGGGG ATGATGCAGA GGAGGGGGAA GTTTCCGACG AAGACAGCGC AGATGAAATT 
GAAGATGAGT GCAAGTTCAA GCTCCATTAC AATAATGGGA CCACTGAGCA TCAGGTGGAG 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTGAAAGAAT CTCAAATCCG AGACAAGGAA 
GATCCCGATA GCTTCACAGT GAGGGCGCTA CTGAGGGCCA CACACGAAGG CCTAAATGCA 
CACCTGAAGC AGCATCCAGA TGTGAAGGAA AATGGGAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGCAGAAA ACCACAAAAT CACGATCTAA GTCTTACAGT 
ACTGACGATG AGGAGGACAC ACAGCAGAAT CCCGGCAAGG AAACCGGCCA GCTGTACAGG 
TTGGGTCGGC GCAGGAAAAC CATGAAGCTC TGCCGAGAGC TCTCTGACTT GGTGGTGTAT 
ACAAACTCCG TGGCCGCCCA GGACATCGTG GATGATGGAA CCACAGGAAA CGTGTTATCA 
TTTAGTGAAA CAAGAGCACA TCAGGTGGTT CAGCAGAAAT CGGAGCAGTT CATGATCTAC 
AACCAAAAGC AGCTCACGAG GATTTACCCG TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCTCTGC CCTACTGGAA CGCAGGCTGC CAGCTAGTGG CTCTGAACTA CCAGTCCGAA 
GGGCGAATGA TGCAATTAAA TCGAGCCAAA TTCAAGACCA ATGGCAATTG TGGCTACGTC 
CTCAAACCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT ACTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAGCAGCT CATCCTGAAA GTGATCAGTG GACAGCAGCT CCCCAAACCT 
CCAGACTCCA TGTTCGGAGA CCGAGGCGAG ATCATTGATC CTTTCGTTGA AGTTGAAATT 
ATTGGATTGC CAGTAGATTG CTGTAAAGAT CAAACCCGTG TAGTAGATGA CAATGGATTT 
AACCCTGTGT GGGAAGAAAC CCTGACGTTT ACAGTACACA TGCCAGACAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCGATT GGACGAGACT TCGTTGGACA AAGAACTGTG 
ACCTTCAGCA GCTTAGTGCC TGGCTACCGG CACGTCTACT TGGAAGGACT GACAGAGGCA 
TCGATATTTG TCCACATAAC AATCAATGAA ATCTATGGGA AGAGCAGACA GCTCCAAGGT 
CTGAAGGGAT TGTTCAATAA GAATCCCAGG CATGGTTCTT CAGAAAACAA TTCCCATTAC 
GTTCGGAAAC GATCAATTGG AGATAGAATT CTACGCCGCA CAGCCAGCGC TCCAGCCAAA 
GGCAGAAAGA AGAGCAAAAT GGGCTTCCAA GAGATGGTAG AGATAAAGGA TTCTGTATCT 
GAGGCTGCGA GAGATCAGGA TGGTGTCCTG AGGAGGACCA CACGGAGCTT GCAAGAGCGC 
CCTGTCTCTC TACCTGTTGA CAAAAGTCTT CTGGGGGCCT TGTCACTGCC CCTATCTGAA 
ACAGCGAAAG ACACTGAAGG AAAAGAAAAC TCTCTGGCAG AAGATAAGGA TGGAAGAAGA 
AAAGGGAAGG CAAGTATAAA AGACCAACAC TTTCCAAATT TCAACAAAAA GTTATCCTCT 
TCCTCTAGCG CTCTCCTCCA CAAAGACCCC CGCCCAGGGG GCTCCGCTGT TTCTGTCGCC 
AGCGCATCCA GCGCATCCGT CACCGGGGAA CAGCTGGGAG CCCCGGGCCT CCGGGGCGGG 
AGAACCAGAT CACCTGCGCC AGGTGACTGC CAGGAGCAGG AGTGTCCCCG CAAACCCCGC 
TCCCCAAGGC AGCATCTGGC TCTGGAACCT GCACTTACCG CAGCGCAAGG TGGTGTGAGC 
ACCCAGGCAC CTGCGCGCGC TGGAGGCCTT GGGGAGGACG GAAGCCCCCG GTCAGGAAGC 
AGGTTTTCTC AAAGCCACCT GGAGGGCAGG GACTTGGAGG GCAGCTGGGT GACGGGCCGA 
GCCGCAGCGT CCCTCTCTCT GTCCGACGTG TCTCTGCTGT GTGCTGCGCA CCCCGACTCG 
CACTCCGCGG CCGTTCTGCA GGAGAGCGAC ATCTCCCATC TTCTTGACAC TGTCCCTCTC 
GCTACCGAGG GCGACCCGGG CAGCTCCATC TCGGCGCTGG TCGGCCAGTG TGAGGACGCC 
GGGGAGCCGG GGAGCCTCGC CGCGGCTCCT CCCCCGCACA GCACCTGCGT GGCGGCAGGT 
CATCCTCCCT TGCCCGCCCT GGACCCCGAG CCGGCCGTGG AGCCCGACGC TGCCGCGGGA 
AAATGCAAGC CCTGCTTCGC GCGCGCATCC CCCGCCCGGA TTCCCGCCAA GGGCCCCGAG 
GCCTCGGCGC ACACGCTTCA CCAGACCACC TGCGCCACTG CCCCGCGGCC GCCCCCGGAG 
GACCAGCTCG CCGCGGGAAG CCACCGGCCC GGGGCCTCGG CCACGTGTCC CTGCCCGCCA 
CCGCGAAGCC CCGGCGCCGG GCAGGTGCGA GGAGCGCCCG GGAGCCGCAG CCCCACCCCC 
GCCCCGGCCC CCGCGGCCCC GCCAGCCGAC CGCGCGCCCC GTTTCCATCC TGGAGACCGC 
AGTGTCGTGG GGGCGGGGGG CGCCGCAGGC CCCGCGGACC TGCCCGCACC TGCCGCTGAA 
CGCCCCGGCG ACGACGCAGG CCCCCTCGCG GCTCCTCTGC AGCCCAGGCG CAGCCCGCAC 
GCGGTGGACC ACGTCCCCGG AGCTCGGGGA AACGGCCGCT GCCCAGACAC CCCGCACCCC 
CGCGTCCCCG CGGCGCCGCA CAGCGCTCCC CAAAGCACCT CTGGCCTCAC CTGCCCAGGT 
GCTGCCGCTC CCACGCGCAG CCGCTGCTCG CACCCGGATG CCCACGCCGA GCAGGCCCGC 
GTGCCCGCGC CCCGCCACGC GCCCCCGGGG CCCGCCCTCA AGCTGCCCAG CCCTTGCAAG 
TCCAAGAGCC TGGGCGACCT GACATCCGAG GACATCGCCT GCAACTTCGA AAGCAAGTAC 
CAGTGCATCA GCAGGAGCTT TGTCGCCACC GGCCTCCGCG ACCAGAAGGC CGTGGCGCTG 
GCGGCCGCCG CCCTGGAGCC CATGGACGCC CTGACGGAGC AGCTGCGGAG GCTGGTGTCC 
TTGGAGCAGG ATGACGGCGG CCAGGTGCTC CGCGCCGAGC GCGACGCCAG CCCGCTGCCC 
CGGGCGCTGG TCCGGAAGCT GTCGTCGCGC AGTCAGAGCA GGGTGCGCAA CATCGCCAGC 
CGGGCCCGGG AGAAGCAGGA GGCCGGCAAG CAGAGGGGCG CCAAGCCCGG CCCCGCGGCG 
GGGGTGGTCC TCCGCCACCG AGCCGCCCCG CCGCCCGCGG GGACCCGGCA CTCCACGGGC 
TCCTACATCG CGGGCTTCCT GCGCGGGGGC GCCGAGGACC GCGGCATCCC CGAGGGCGCC 
TGCGCAGCGC TGCGCCCCGC CCCCGGAGAC CACGCCCCCG GAGGCCCCGC CCCCGCCGCC 
CGCTTCTGCT CCCACGGCTC CGTCCTGCAC ACCGAGCCCG GCGGCGGCGG CGACGACAAG 
CCCGAGATCT ATTTTCTCTT GAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCH1
Entrez Gene ID	100684810
Full Name	phospholipase C eta 1
General protein information	Preferred Names phospholipase C eta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Pan troglodytes (chimpanzee)	PLCH1	XP_001149022.1
	LOC707243	XP_002808381.1
Bos taurus (cattle)	PLCH1	XP_002685035.3
Rattus norvegicus (Norway rat)	Plch1	NP_001178636.1
Xenopus tropicalis (tropical clawed frog)	plch1	XP_002933304.2
Homo sapiens (human)	PLCH1	NP_001124432.1
Gallus gallus (chicken)	PLCH1	XP_004943620.1
Danio rerio (zebrafish)	LOC566479	XP_694841.5
Mus musculus (house mouse)	Plch1	NP_899014.2
Canis lupus familiaris (dog)	PLCH1	XP_005634651.1

Next-day Shipping ORF Clones ( in default vector with tag)
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PLCH1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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