UNC13B gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005626766.2	XP_005626823.1	protein unc-13 homolog B isoform X3
XM_014117834.1	XP_013973309.1	protein unc-13 homolog B isoform X9
XM_014117837.2	XP_013973312.1	protein unc-13 homolog B isoform X13
XM_014117831.1	XP_013973306.1	protein unc-13 homolog B isoform X7
XM_014117836.1	XP_013973311.1	protein unc-13 homolog B isoform X11
XM_014117838.1	XP_013973313.1	protein unc-13 homolog B isoform X14
XM_022425553.1	XP_022281261.1	protein unc-13 homolog B isoform X12
XM_005626767.2	XP_005626824.1	protein unc-13 homolog B isoform X4
XM_014117829.1	XP_013973304.1	protein unc-13 homolog B isoform X5
XM_005626768.2	XP_005626825.1	protein unc-13 homolog B isoform X12
XM_005626764.3	XP_005626821.1	protein unc-13 homolog B isoform X1
XM_005626767.3	XP_005626824.1	protein unc-13 homolog B isoform X4
XM_005626768.3	XP_005626825.1	protein unc-13 homolog B isoform X5
XM_022425550.1	XP_022281258.1	protein unc-13 homolog B isoform X9
XM_014117837.1	XP_013973312.1	protein unc-13 homolog B isoform X13
XM_005626764.2	XP_005626821.1	protein unc-13 homolog B isoform X1
XM_005626765.2	XP_005626822.1	protein unc-13 homolog B isoform X2
XM_014117835.1	XP_013973310.1	protein unc-13 homolog B isoform X10
XM_005626766.3	XP_005626823.1	protein unc-13 homolog B isoform X3
XM_022425547.1	XP_022281255.1	protein unc-13 homolog B isoform X6
XM_022425548.1	XP_022281256.1	protein unc-13 homolog B isoform X7
XM_022425551.1	XP_022281259.1	protein unc-13 homolog B isoform X10
XM_014117830.1	XP_013973305.1	protein unc-13 homolog B isoform X6
XM_014117833.1	XP_013973308.1	protein unc-13 homolog B isoform X8
XM_005626765.3	XP_005626822.1	protein unc-13 homolog B isoform X2
XM_022425549.1	XP_022281257.1	protein unc-13 homolog B isoform X8
XM_022425552.1	XP_022281260.1	protein unc-13 homolog B isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe292143	XM_022425552.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe292144	XM_022425553.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe39193	XM_014117837.2 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe39194	XM_014117838.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe14576	XM_022425547.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe14577	XM_022425548.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe14578	XM_022425549.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe14579	XM_022425550.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe292142	XM_022425551.1 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39186	XM_014117829.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe39187	XM_014117830.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe39188	XM_014117831.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39189	XM_014117833.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39190	XM_014117834.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39191	XM_014117835.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39192	XM_014117836.1 Latest version!	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe14570	XM_005626764.3 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14571	XM_005626765.3 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14572	XM_005626766.3 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14573	XM_005626767.3 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14574	XM_005626768.3 Latest version!	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe14573	XM_005626767.2	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14574	XM_005626768.2	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe39193	XM_014117837.1	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe14570	XM_005626764.2	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14572	XM_005626766.2	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe14571	XM_005626765.2	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

UNC13B ( XM_022425552.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281260.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe292143
Clone ID Related Accession (Same CDS sequence)	XM_022425552.1
Accession Version	XM_022425552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAT CTGTGGAACT AAAAACCCAA CTCCTCATAA GATTTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGTATTC TATTCAAGAA GAAAGCCAGA GGAAGCCATT GCCCACTGCT 
GCCGCCCAGT GTTGTCACTG GACCTATTTG GGCTGGGGAG AGCAGCAGAC TTTTGAAGAT 
CCTGATAGTG CTGTTGATGA CCGGGATAGT GACTATCGCA GTGAGACCAG CAATAGCATC 
CCACCTCCTT ATCATACAAC TTCCCAGCCC AACGCTTCTG TGCACCAGTT TCCTGTGCCC 
ATGCGATTGC CACAGCAGCT GCTACTTCAG GGCAATTCCC GGGATTCTTG CAATGACTCC 
ATGCAAAGTT ACGATCTTGA TTATCCAGAG CGGCGGGCCC TTAGCCCCAC CAGCAGTAGT 
AGGTATGGCT CATCCTGTAA TGTGAGTCAA GGAAGCTCTC AGTTGAGTGA ACTAGACCAG 
TGTCACGAAC AAGATGATGA CCGTCGGGAG AGGGATTCAA TTCATTCATG CCACAGCTCT 
GGCAGCTTCT CCAGAGACGG CCAGGTGGGC TTTGGAGAAC AAGAGAAAGC CTTGGAGGTA 
ACAGGTGAAG AAGAGAAGGG GAGAGTGTGT GAACCCAAGG ACAAGAAAAA AGATACCACA 
ACCCATCTTC CCCCAGATCT GGTGCTACCC AAAGACCACG TCCTAGGCCC CCAGGAGAGT 
TTTCCTGAGG AGAATGGTTC TTCACCATTT ACCCAAGCAA GAGCTCACTG GATCCGAGCA 
GTTACCAAGG TTCGACTCCA GCTGCAGGAG ATTCCGGATG ATGGTCACCC TTCTTTGCCC 
CAGTGGCTTC CGGAAGGGCC TGCTGGAGGG CTCTATGGCA TTGACAGCAT GCCAGATCTA 
CGCAGAAAGA AACCATTGCC ACTTGTCAGT GATCTGGCTA TGTCATTGGT CCAGTCCCGG 
AAAGCAGGAA TTACTTCTGC AATGGCTACA CGTACCTCTC TGAAGGATGA AGAGCTGAAA 
TCCCATGTGT ATAAGAAAAC CCTGCAGGCC TTAATCTACC CAATCTCGTG CACCACACCC 
CACAACTTTG AAGTCTGGAC AGCCACTACC CCAACCTACT GCTATGAGTG TGAAGGTCTG 
CTCTGGGGCA TTGCCCGGCA GGGCATGCGC TGCAGCGAGT GTGGAGTCAA GTGCCATGAG 
AAGTGCCAAG ACCTGCTCAA CGCCGACTGC CTGCAGCGGG CTGCAGAAAA GAGTTCTAAA 
CATGGAGCTG AGGACCGGAC CCAGAACATA ATCATGGCGA TGAAGGACCG CATGAAGATC 
CGAGAGCGGA ATAAGCCAGA GATCTTTGAG GTTATCCGAG ATGTCTTTAC CGTGAGCAAA 
GTTGCCCATG TGCAACAGAT GAAAACAGTG AAGCAGAGTG TACTGGATGG CACCTCCAAA 
TGGTCAGCCA AAATTACCAT CACTGTGGTG TGTGCCCAGG GCCTACAAGC CAAGGACAAG 
ACAGGATCCA GTGACCCTTA TGTGACTGTG CAAGTGGGCA AAACCAAGAA GCGTACCAAG 
ACTATTTTTG GGAACCTGAA TCCTGTTTGG GAAGAGAAGT TCCATTTTGA GTGCCACAAC 
TCTTCTGACC GCATTAAGGT ACGTGTATGG GATGAGGATG ATGACATCAA GTCAAGAGTA 
AAGCAACGGC TAAAGCGAGA GTCCGATGAT TTCCTTGGCC AAACCATTAT TGAGGTTCGG 
ACACTGAGTG GCGAGATGGA CGTCTGGTAC AACTTGGAGA AGAGGACAGA TAAATCAGCT 
GTCTCAGGGG CTATCCGACT GCAAATCAGT GTGGAGATCA AGGGAGAGGA GAAAGTAGCG 
CCATATCATG TGCAGTATAC GTGTCTCCAT GAGAACCTTT TCCATTACCT CACAGACATT 
CAGGGTAGTG GAGGAGTCCT GATCCCTGAG GCTCGCGGAG ACGATGCATG GAAGGTGTAC 
TTTGATGAGA CTGCCCAAGA AATTGTGGAT GAATTTGCCA TGCGCTATGG CATCGAGTCC 
ATATATCAGG CCATGACGCA CTTTGCATGT TTGTCATCCA AGTACATGTG TCCTGGTGTG 
CCTGCGGTGA TGAGCACCTT ATTGGCCAAC ATCAATGCCT ACTATGCCCA TACAACTGCC 
TCTACCAACG TCTCTGCATC TGATCGCTTT GCAGCCTCCA ACTTTGGGAA AGAGAGATTT 
GTAAAACTAC TGGACCAGCT GCACAACTCA CTGAGGATTG ACCTCTCTAC CTACAGGAAT 
AATTTCCCTG CTGGAAGCCC TGAGCGGCTT CAGGACTTAA AGTCAACAGT GGATTTGCTG 
ACCAGCATTA CTTTCTTCAG AATGAAGGTA CAAGAACTGC AGAGCCCTCC AAGAGCCAGC 
CAAGTGGTAA AGGATTGTGT GAAGGCCTGT TTGAACTCCA CATATGAGTA TATCTTCAAC 
AACTGCCATG ACTTGTATAG TCGCCAGTAC CAGCTGAAAC AGGAGCTACC TCCAGAGGAA 
CAAGGACCCA GCATTCGGAA CCTGGATTTT TGGCCCAAAC TCATCACGCT CATTGTGTCA 
ATCATAGAGG AAGATAAGAA TTCCTACACA CTCGTTCTGA ACCAGTTTCC TCAGGAATTG 
AATGTGGGAA AAGTCAGCGC AGAAGTGATG TGGCAGCTCT TTGCCCAAGA CATGAAATAT 
GCACTGGAGG AGCATGAGAA GGACCGGCTG TGTAAGAGTG CTGACTACAT GAACCTGCAC 
TTCAAGGTGA AGTGGCTCCA CAATGAATAC GTGCGAGATC TGCCTGCCCT GCAGGGGCAG 
GTGCCTGAGT ACCCAGTGTG GTTTGAGCAG TTTGTTCTGC AATGGCTGGA TGAAAATGAG 
GATGTGTCCC TGGAATTCCT GCGTGGGGCT CTGGAACGAG ATAAGAAGGA TGGGTTCCAG 
CAGACATCAG AGCATGCACT CTTTTCCTGC TCTGTGGTGG ATGTCTTCAC ACAGCTCAAC 
CAGAGCTTTG AGATCATCCG GAAGCTGGAG TGCCCAGACC CCAACATCCT TGCCCACTAC 
ATGAGGAGAT TTGCTAAGAC CATCGGGAAG GTGTTGATGC AGTATGCAAA CATCTTATCA 
AAGAGCTTCC CAGATTATTG CACAAAGGAG AAAATGCCCT GCATCCTGAT GAACAACATG 
CAGCAACTGA GGGTCCAGCT GGAGAAAATG TTTGAGGCCA TGGGAGGCAA GGAGTTGGAC 
CCTGAAGCTG CAGACAGTTT GAAGGAGCTG CAGGTGAAAC TGAATACAGT TCTGGATGAG 
CTCAGCATGG TATTTGGAAA CAGTTTTCAG GTGCGGATTG ATGAGTGTGT TCGACAAATG 
GCTGACATCC TGGGCCAGGT GCGAGGCCCA GGGAATGCAT CCCCCAACGC CAGGGCCTCT 
GTGGCTCAGG ATGCAGATAG TGTGCTCCGG CCTCTCATGG ACTTCCTGGA TGGCAACCTC 
ACACTCTTTG CCACTGTGTG TGAGAAGACA GTCCTGAAGC GTGTACTGAA GGAGCTCTGG 
AGGGTGGTCA TGAACACAAT GGAAAGAATG ATTGTTCTGC CCCCACTCAC TGACCAGACG 
GGCACCCAGC TGATCTTTAC TGCTGCCAAG GAGCTGAGCC ATCTTTCTAA GCTCAAGGAC 
CACATGGTGC GAGAGGAAAC ACGGAGTCTC ACTCCAAAGC AGTGTGAAGT CCTTGACCTC 
GCTCTGGACA CTATCAAGCA ATACTTTCAT GCAGGAGGTA ATGGGCTGAA GAAAACCTTC 
CTGGAGAAGA GCTCAGATCT GCAGTCTCTA CGCTATGCCC TGTCTCTCTA CACACAGACC 
ACAGATACAC TCATCAAAAC CTTTGTACGC TCACAGACTG CCCAGGTGCA TGATGGGAAA 
GGTATTAGGT TTACTGCTAA TGAGGACATT CGGCCGGAAA AGGGATCTGG TGTGGATGAT 
CCTGTGGGAG AAGTCTCTAT TCAGGTGGAC TTGTTTACAC ATCCTGGAAC TGGGGAGCAC 
AAGGTCACAG TGAAAGTGGT AGCTGCCAAT GACCTCAAGT GGCAGACAGC GGGTATGTTC 
CGGCCCTTTG TGGAAGTGAC CATGGTTGGC CCACACCAAA GTGAAAAGAA GAGGAAGTTC 
ACAACCAAGT CAAAAAGCAA CAACTGGGCT CCCAAGTACA ATGAGACATT TCACTTCCTC 
CTGGGAAATG AAGAAGGGCC AGAAGCCTAT GAATTGCAAA TATGTGTGAA GGATTACTGC 
TTTGCCCGTG AGGATCGTGT TTTGGGGCTG GCTGTGATGC CTCTGAGGGA TGTAGCAGCC 
AAGGGCAGCT GTGCTTGCTG GTGTCCCTTG GGCCGGAAGA TCCACATGGA TGAGACAGGC 
ATGACTATTC TCCGGATTCT ATCTCAAAGG AGCAATGACG AAGTGGCTCG AGAGTTTGTG 
AAGCTCAAAT CGGAGTCTCG TTCCATGGAA GAGGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281260.1
CDS	168..4649
Translation	MMRSVELKTQLLIRFCLIQDLSCLLYSIQEESQRKPLPTAAAQCCHWTYLGWGEQQTFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425552.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425552.1

ORF Insert Sequence:

ATGATGAGAT CTGTGGAACT AAAAACCCAA CTCCTCATAA GATTTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGTATTC TATTCAAGAA GAAAGCCAGA GGAAGCCATT GCCCACTGCT 
GCCGCCCAGT GTTGTCACTG GACCTATTTG GGCTGGGGAG AGCAGCAGAC TTTTGAAGAT 
CCTGATAGTG CTGTTGATGA CCGGGATAGT GACTATCGCA GTGAGACCAG CAATAGCATC 
CCACCTCCTT ATCATACAAC TTCCCAGCCC AACGCTTCTG TGCACCAGTT TCCTGTGCCC 
ATGCGATTGC CACAGCAGCT GCTACTTCAG GGCAATTCCC GGGATTCTTG CAATGACTCC 
ATGCAAAGTT ACGATCTTGA TTATCCAGAG CGGCGGGCCC TTAGCCCCAC CAGCAGTAGT 
AGGTATGGCT CATCCTGTAA TGTGAGTCAA GGAAGCTCTC AGTTGAGTGA ACTAGACCAG 
TGTCACGAAC AAGATGATGA CCGTCGGGAG AGGGATTCAA TTCATTCATG CCACAGCTCT 
GGCAGCTTCT CCAGAGACGG CCAGGTGGGC TTTGGAGAAC AAGAGAAAGC CTTGGAGGTA 
ACAGGTGAAG AAGAGAAGGG GAGAGTGTGT GAACCCAAGG ACAAGAAAAA AGATACCACA 
ACCCATCTTC CCCCAGATCT GGTGCTACCC AAAGACCACG TCCTAGGCCC CCAGGAGAGT 
TTTCCTGAGG AGAATGGTTC TTCACCATTT ACCCAAGCAA GAGCTCACTG GATCCGAGCA 
GTTACCAAGG TTCGACTCCA GCTGCAGGAG ATTCCGGATG ATGGTCACCC TTCTTTGCCC 
CAGTGGCTTC CGGAAGGGCC TGCTGGAGGG CTCTATGGCA TTGACAGCAT GCCAGATCTA 
CGCAGAAAGA AACCATTGCC ACTTGTCAGT GATCTGGCTA TGTCATTGGT CCAGTCCCGG 
AAAGCAGGAA TTACTTCTGC AATGGCTACA CGTACCTCTC TGAAGGATGA AGAGCTGAAA 
TCCCATGTGT ATAAGAAAAC CCTGCAGGCC TTAATCTACC CAATCTCGTG CACCACACCC 
CACAACTTTG AAGTCTGGAC AGCCACTACC CCAACCTACT GCTATGAGTG TGAAGGTCTG 
CTCTGGGGCA TTGCCCGGCA GGGCATGCGC TGCAGCGAGT GTGGAGTCAA GTGCCATGAG 
AAGTGCCAAG ACCTGCTCAA CGCCGACTGC CTGCAGCGGG CTGCAGAAAA GAGTTCTAAA 
CATGGAGCTG AGGACCGGAC CCAGAACATA ATCATGGCGA TGAAGGACCG CATGAAGATC 
CGAGAGCGGA ATAAGCCAGA GATCTTTGAG GTTATCCGAG ATGTCTTTAC CGTGAGCAAA 
GTTGCCCATG TGCAACAGAT GAAAACAGTG AAGCAGAGTG TACTGGATGG CACCTCCAAA 
TGGTCAGCCA AAATTACCAT CACTGTGGTG TGTGCCCAGG GCCTACAAGC CAAGGACAAG 
ACAGGATCCA GTGACCCTTA TGTGACTGTG CAAGTGGGCA AAACCAAGAA GCGTACCAAG 
ACTATTTTTG GGAACCTGAA TCCTGTTTGG GAAGAGAAGT TCCATTTTGA GTGCCACAAC 
TCTTCTGACC GCATTAAGGT ACGTGTATGG GATGAGGATG ATGACATCAA GTCAAGAGTA 
AAGCAACGGC TAAAGCGAGA GTCCGATGAT TTCCTTGGCC AAACCATTAT TGAGGTTCGG 
ACACTGAGTG GCGAGATGGA CGTCTGGTAC AACTTGGAGA AGAGGACAGA TAAATCAGCT 
GTCTCAGGGG CTATCCGACT GCAAATCAGT GTGGAGATCA AGGGAGAGGA GAAAGTAGCG 
CCATATCATG TGCAGTATAC GTGTCTCCAT GAGAACCTTT TCCATTACCT CACAGACATT 
CAGGGTAGTG GAGGAGTCCT GATCCCTGAG GCTCGCGGAG ACGATGCATG GAAGGTGTAC 
TTTGATGAGA CTGCCCAAGA AATTGTGGAT GAATTTGCCA TGCGCTATGG CATCGAGTCC 
ATATATCAGG CCATGACGCA CTTTGCATGT TTGTCATCCA AGTACATGTG TCCTGGTGTG 
CCTGCGGTGA TGAGCACCTT ATTGGCCAAC ATCAATGCCT ACTATGCCCA TACAACTGCC 
TCTACCAACG TCTCTGCATC TGATCGCTTT GCAGCCTCCA ACTTTGGGAA AGAGAGATTT 
GTAAAACTAC TGGACCAGCT GCACAACTCA CTGAGGATTG ACCTCTCTAC CTACAGGAAT 
AATTTCCCTG CTGGAAGCCC TGAGCGGCTT CAGGACTTAA AGTCAACAGT GGATTTGCTG 
ACCAGCATTA CTTTCTTCAG AATGAAGGTA CAAGAACTGC AGAGCCCTCC AAGAGCCAGC 
CAAGTGGTAA AGGATTGTGT GAAGGCCTGT TTGAACTCCA CATATGAGTA TATCTTCAAC 
AACTGCCATG ACTTGTATAG TCGCCAGTAC CAGCTGAAAC AGGAGCTACC TCCAGAGGAA 
CAAGGACCCA GCATTCGGAA CCTGGATTTT TGGCCCAAAC TCATCACGCT CATTGTGTCA 
ATCATAGAGG AAGATAAGAA TTCCTACACA CTCGTTCTGA ACCAGTTTCC TCAGGAATTG 
AATGTGGGAA AAGTCAGCGC AGAAGTGATG TGGCAGCTCT TTGCCCAAGA CATGAAATAT 
GCACTGGAGG AGCATGAGAA GGACCGGCTG TGTAAGAGTG CTGACTACAT GAACCTGCAC 
TTCAAGGTGA AGTGGCTCCA CAATGAATAC GTGCGAGATC TGCCTGCCCT GCAGGGGCAG 
GTGCCTGAGT ACCCAGTGTG GTTTGAGCAG TTTGTTCTGC AATGGCTGGA TGAAAATGAG 
GATGTGTCCC TGGAATTCCT GCGTGGGGCT CTGGAACGAG ATAAGAAGGA TGGGTTCCAG 
CAGACATCAG AGCATGCACT CTTTTCCTGC TCTGTGGTGG ATGTCTTCAC ACAGCTCAAC 
CAGAGCTTTG AGATCATCCG GAAGCTGGAG TGCCCAGACC CCAACATCCT TGCCCACTAC 
ATGAGGAGAT TTGCTAAGAC CATCGGGAAG GTGTTGATGC AGTATGCAAA CATCTTATCA 
AAGAGCTTCC CAGATTATTG CACAAAGGAG AAAATGCCCT GCATCCTGAT GAACAACATG 
CAGCAACTGA GGGTCCAGCT GGAGAAAATG TTTGAGGCCA TGGGAGGCAA GGAGTTGGAC 
CCTGAAGCTG CAGACAGTTT GAAGGAGCTG CAGGTGAAAC TGAATACAGT TCTGGATGAG 
CTCAGCATGG TATTTGGAAA CAGTTTTCAG GTGCGGATTG ATGAGTGTGT TCGACAAATG 
GCTGACATCC TGGGCCAGGT GCGAGGCCCA GGGAATGCAT CCCCCAACGC CAGGGCCTCT 
GTGGCTCAGG ATGCAGATAG TGTGCTCCGG CCTCTCATGG ACTTCCTGGA TGGCAACCTC 
ACACTCTTTG CCACTGTGTG TGAGAAGACA GTCCTGAAGC GTGTACTGAA GGAGCTCTGG 
AGGGTGGTCA TGAACACAAT GGAAAGAATG ATTGTTCTGC CCCCACTCAC TGACCAGACG 
GGCACCCAGC TGATCTTTAC TGCTGCCAAG GAGCTGAGCC ATCTTTCTAA GCTCAAGGAC 
CACATGGTGC GAGAGGAAAC ACGGAGTCTC ACTCCAAAGC AGTGTGAAGT CCTTGACCTC 
GCTCTGGACA CTATCAAGCA ATACTTTCAT GCAGGAGGTA ATGGGCTGAA GAAAACCTTC 
CTGGAGAAGA GCTCAGATCT GCAGTCTCTA CGCTATGCCC TGTCTCTCTA CACACAGACC 
ACAGATACAC TCATCAAAAC CTTTGTACGC TCACAGACTG CCCAGGTGCA TGATGGGAAA 
GGTATTAGGT TTACTGCTAA TGAGGACATT CGGCCGGAAA AGGGATCTGG TGTGGATGAT 
CCTGTGGGAG AAGTCTCTAT TCAGGTGGAC TTGTTTACAC ATCCTGGAAC TGGGGAGCAC 
AAGGTCACAG TGAAAGTGGT AGCTGCCAAT GACCTCAAGT GGCAGACAGC GGGTATGTTC 
CGGCCCTTTG TGGAAGTGAC CATGGTTGGC CCACACCAAA GTGAAAAGAA GAGGAAGTTC 
ACAACCAAGT CAAAAAGCAA CAACTGGGCT CCCAAGTACA ATGAGACATT TCACTTCCTC 
CTGGGAAATG AAGAAGGGCC AGAAGCCTAT GAATTGCAAA TATGTGTGAA GGATTACTGC 
TTTGCCCGTG AGGATCGTGT TTTGGGGCTG GCTGTGATGC CTCTGAGGGA TGTAGCAGCC 
AAGGGCAGCT GTGCTTGCTG GTGTCCCTTG GGCCGGAAGA TCCACATGGA TGAGACAGGC 
ATGACTATTC TCCGGATTCT ATCTCAAAGG AGCAATGACG AAGTGGCTCG AGAGTTTGTG 
AAGCTCAAAT CGGAGTCTCG TTCCATGGAA GAGGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_022425553.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281261.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe292144
Clone ID Related Accession (Same CDS sequence)	XM_022425553.1
Accession Version	XM_022425553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAT CTGTGGAACT AAAAACCCAA CTCCTCATAA GATTTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGTATTC TATTCAAGAA GAAAGCCAGA GGAAGCCATT GCCCACTGCT 
GCCGCCCAGT GTTCTTTTGA AGATCCTGAT AGTGCTGTTG ATGACCGGGA TAGTGACTAT 
CGCAGTGAGA CCAGCAATAG CATCCCACCT CCTTATCATA CAACTTCCCA GCCCAACGCT 
TCTGTGCACC AGTTTCCTGT GCCCATGCGA TTGCCACAGC AGCTGCTACT TCAGGGCAAT 
TCCCGGGATT CTTGCAATGA CTCCATGCAA AGTTACGATC TTGATTATCC AGAGCGGCGG 
GCCCTTAGCC CCACCAGCAG TAGTAGGTAT GGCTCATCCT GTAATGTGAG TCAAGGAAGC 
TCTCAGTTGA GTGAACTAGA CCAGTGTCAC GAACAAGATG ATGACCGTCG GGAGAGGGAT 
TCAATTCATT CATGCCACAG CTCTGGCAGC TTCTCCAGAG ACGGCCAGGT GGGCTTTGGA 
GAACAAGAGA AAGCCTTGGA GGTAACAGGT GAAGAAGAGA AGGGGAGAGT GTGTGAACCC 
AAGGACAAGA AAAAAGATAC CACAACCCAT CTTCCCCCAG ATCTGGTGCT ACCCAAAGAC 
CACGTCCTAG GCCCCCAGGA GAGTTTTCCT GAGGAGAATG GTTCTTCACC ATTTACCCAA 
GCAAGAGCTC ACTGGATCCG AGCAGTTACC AAGGTTCGAC TCCAGCTGCA GGAGATTCCG 
GATGATGGTC ACCCTTCTTT GCCCCAGTGG CTTCCGGAAG GGCCTGCTGG AGGGCTCTAT 
GGCATTGACA GCATGCCAGA TCTACGCAGA AAGAAACCAT TGCCACTTGT CAGTGATCTG 
GCTATGTCAT TGGTCCAGTC CCGGAAAGCA GGAATTACTT CTGCAATGGC TACACGTACC 
TCTCTGAAGG ATGAAGAGCT GAAATCCCAT GTGTATAAGA AAACCCTGCA GGCCTTAATC 
TACCCAATCT CGTGCACCAC ACCCCACAAC TTTGAAGTCT GGACAGCCAC TACCCCAACC 
TACTGCTATG AGTGTGAAGG TCTGCTCTGG GGCATTGCCC GGCAGGGCAT GCGCTGCAGC 
GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC CAAGACCTGC TCAACGCCGA CTGCCTGCAG 
CGGGCTGCAG AAAAGAGTTC TAAACATGGA GCTGAGGACC GGACCCAGAA CATAATCATG 
GCGATGAAGG ACCGCATGAA GATCCGAGAG CGGAATAAGC CAGAGATCTT TGAGGTTATC 
CGAGATGTCT TTACCGTGAG CAAAGTTGCC CATGTGCAAC AGATGAAAAC AGTGAAGCAG 
AGTGTACTGG ATGGCACCTC CAAATGGTCA GCCAAAATTA CCATCACTGT GGTGTGTGCC 
CAGGGCCTAC AAGCCAAGGA CAAGACAGGA TCCAGTGACC CTTATGTGAC TGTGCAAGTG 
GGCAAAACCA AGAAGCGTAC CAAGACTATT TTTGGGAACC TGAATCCTGT TTGGGAAGAG 
AAGTTCCATT TTGAGTGCCA CAACTCTTCT GACCGCATTA AGGTACGTGT ATGGGATGAG 
GATGATGACA TCAAGTCAAG AGTAAAGCAA CGGCTAAAGC GAGAGTCCGA TGATTTCCTT 
GGCCAAACCA TTATTGAGGT TCGGACACTG AGTGGCGAGA TGGACGTCTG GTACAACTTG 
GAGAAGAGGA CAGATAAATC AGCTGTCTCA GGGGCTATCC GACTGCAAAT CAGTGTGGAG 
ATCAAGGGAG AGGAGAAAGT AGCGCCATAT CATGTGCAGT ATACGTGTCT CCATGAGAAC 
CTTTTCCATT ACCTCACAGA CATTCAGGGT AGTGGAGGAG TCCTGATCCC TGAGGCTCGC 
GGAGACGATG CATGGAAGGT GTACTTTGAT GAGACTGCCC AAGAAATTGT GGATGAATTT 
GCCATGCGCT ATGGCATCGA GTCCATATAT CAGGCCATGA CGCACTTTGC ATGTTTGTCA 
TCCAAGTACA TGTGTCCTGG TGTGCCTGCG GTGATGAGCA CCTTATTGGC CAACATCAAT 
GCCTACTATG CCCATACAAC TGCCTCTACC AACGTCTCTG CATCTGATCG CTTTGCAGCC 
TCCAACTTTG GGAAAGAGAG ATTTGTAAAA CTACTGGACC AGCTGCACAA CTCACTGAGG 
ATTGACCTCT CTACCTACAG GAATAATTTC CCTGCTGGAA GCCCTGAGCG GCTTCAGGAC 
TTAAAGTCAA CAGTGGATTT GCTGACCAGC ATTACTTTCT TCAGAATGAA GGTACAAGAA 
CTGCAGAGCC CTCCAAGAGC CAGCCAAGTG GTAAAGGATT GTGTGAAGGC CTGTTTGAAC 
TCCACATATG AGTATATCTT CAACAACTGC CATGACTTGT ATAGTCGCCA GTACCAGCTG 
AAACAGGAGC TACCTCCAGA GGAACAAGGA CCCAGCATTC GGAACCTGGA TTTTTGGCCC 
AAACTCATCA CGCTCATTGT GTCAATCATA GAGGAAGATA AGAATTCCTA CACACTCGTT 
CTGAACCAGT TTCCTCAGGA ATTGAATGTG GGAAAAGTCA GCGCAGAAGT GATGTGGCAG 
CTCTTTGCCC AAGACATGAA ATATGCACTG GAGGAGCATG AGAAGGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATACGTGCGA 
GATCTGCCTG CCCTGCAGGG GCAGGTGCCT GAGTACCCAG TGTGGTTTGA GCAGTTTGTT 
CTGCAATGGC TGGATGAAAA TGAGGATGTG TCCCTGGAAT TCCTGCGTGG GGCTCTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CACTCTTTTC CTGCTCTGTG 
GTGGATGTCT TCACACAGCT CAACCAGAGC TTTGAGATCA TCCGGAAGCT GGAGTGCCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGG AGATTTGCTA AGACCATCGG GAAGGTGTTG 
ATGCAGTATG CAAACATCTT ATCAAAGAGC TTCCCAGATT ATTGCACAAA GGAGAAAATG 
CCCTGCATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAGGAGTT GGACCCTGAA GCTGCAGACA GTTTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTCAGC ATGGTATTTG GAAACAGTTT TCAGGTGCGG 
ATTGATGAGT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AGGTGCGAGG CCCAGGGAAT 
GCATCCCCCA ACGCCAGGGC CTCTGTGGCT CAGGATGCAG ATAGTGTGCT CCGGCCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACACTC TTTGCCACTG TGTGTGAGAA GACAGTCCTG 
AAGCGTGTAC TGAAGGAGCT CTGGAGGGTG GTCATGAACA CAATGGAAAG AATGATTGTT 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TTACTGCTGC CAAGGAGCTG 
AGCCATCTTT CTAAGCTCAA GGACCACATG GTGCGAGAGG AAACACGGAG TCTCACTCCA 
AAGCAGTGTG AAGTCCTTGA CCTCGCTCTG GACACTATCA AGCAATACTT TCATGCAGGA 
GGTAATGGGC TGAAGAAAAC CTTCCTGGAG AAGAGCTCAG ATCTGCAGTC TCTACGCTAT 
GCCCTGTCTC TCTACACACA GACCACAGAT ACACTCATCA AAACCTTTGT ACGCTCACAG 
ACTGCCCAGG TGCATGATGG GAAAGGTATT AGGTTTACTG CTAATGAGGA CATTCGGCCG 
GAAAAGGGAT CTGGTGTGGA TGATCCTGTG GGAGAAGTCT CTATTCAGGT GGACTTGTTT 
ACACATCCTG GAACTGGGGA GCACAAGGTC ACAGTGAAAG TGGTAGCTGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCC TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGAAA AGAAGAGGAA GTTCACAACC AAGTCAAAAA GCAACAACTG GGCTCCCAAG 
TACAATGAGA CATTTCACTT CCTCCTGGGA AATGAAGAAG GGCCAGAAGC CTATGAATTG 
CAAATATGTG TGAAGGATTA CTGCTTTGCC CGTGAGGATC GTGTTTTGGG GCTGGCTGTG 
ATGCCTCTGA GGGATGTAGC AGCCAAGGGC AGCTGTGCTT GCTGGTGTCC CTTGGGCCGG 
AAGATCCACA TGGATGAGAC AGGCATGACT ATTCTCCGGA TTCTATCTCA AAGGAGCAAT 
GACGAAGTGG CTCGAGAGTT TGTGAAGCTC AAATCGGAGT CTCGTTCCAT GGAAGAGGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281261.1
CDS	168..4613
Translation	MMRSVELKTQLLIRFCLIQDLSCLLYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425553.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425553.1

ORF Insert Sequence:

ATGATGAGAT CTGTGGAACT AAAAACCCAA CTCCTCATAA GATTTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGTATTC TATTCAAGAA GAAAGCCAGA GGAAGCCATT GCCCACTGCT 
GCCGCCCAGT GTTCTTTTGA AGATCCTGAT AGTGCTGTTG ATGACCGGGA TAGTGACTAT 
CGCAGTGAGA CCAGCAATAG CATCCCACCT CCTTATCATA CAACTTCCCA GCCCAACGCT 
TCTGTGCACC AGTTTCCTGT GCCCATGCGA TTGCCACAGC AGCTGCTACT TCAGGGCAAT 
TCCCGGGATT CTTGCAATGA CTCCATGCAA AGTTACGATC TTGATTATCC AGAGCGGCGG 
GCCCTTAGCC CCACCAGCAG TAGTAGGTAT GGCTCATCCT GTAATGTGAG TCAAGGAAGC 
TCTCAGTTGA GTGAACTAGA CCAGTGTCAC GAACAAGATG ATGACCGTCG GGAGAGGGAT 
TCAATTCATT CATGCCACAG CTCTGGCAGC TTCTCCAGAG ACGGCCAGGT GGGCTTTGGA 
GAACAAGAGA AAGCCTTGGA GGTAACAGGT GAAGAAGAGA AGGGGAGAGT GTGTGAACCC 
AAGGACAAGA AAAAAGATAC CACAACCCAT CTTCCCCCAG ATCTGGTGCT ACCCAAAGAC 
CACGTCCTAG GCCCCCAGGA GAGTTTTCCT GAGGAGAATG GTTCTTCACC ATTTACCCAA 
GCAAGAGCTC ACTGGATCCG AGCAGTTACC AAGGTTCGAC TCCAGCTGCA GGAGATTCCG 
GATGATGGTC ACCCTTCTTT GCCCCAGTGG CTTCCGGAAG GGCCTGCTGG AGGGCTCTAT 
GGCATTGACA GCATGCCAGA TCTACGCAGA AAGAAACCAT TGCCACTTGT CAGTGATCTG 
GCTATGTCAT TGGTCCAGTC CCGGAAAGCA GGAATTACTT CTGCAATGGC TACACGTACC 
TCTCTGAAGG ATGAAGAGCT GAAATCCCAT GTGTATAAGA AAACCCTGCA GGCCTTAATC 
TACCCAATCT CGTGCACCAC ACCCCACAAC TTTGAAGTCT GGACAGCCAC TACCCCAACC 
TACTGCTATG AGTGTGAAGG TCTGCTCTGG GGCATTGCCC GGCAGGGCAT GCGCTGCAGC 
GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC CAAGACCTGC TCAACGCCGA CTGCCTGCAG 
CGGGCTGCAG AAAAGAGTTC TAAACATGGA GCTGAGGACC GGACCCAGAA CATAATCATG 
GCGATGAAGG ACCGCATGAA GATCCGAGAG CGGAATAAGC CAGAGATCTT TGAGGTTATC 
CGAGATGTCT TTACCGTGAG CAAAGTTGCC CATGTGCAAC AGATGAAAAC AGTGAAGCAG 
AGTGTACTGG ATGGCACCTC CAAATGGTCA GCCAAAATTA CCATCACTGT GGTGTGTGCC 
CAGGGCCTAC AAGCCAAGGA CAAGACAGGA TCCAGTGACC CTTATGTGAC TGTGCAAGTG 
GGCAAAACCA AGAAGCGTAC CAAGACTATT TTTGGGAACC TGAATCCTGT TTGGGAAGAG 
AAGTTCCATT TTGAGTGCCA CAACTCTTCT GACCGCATTA AGGTACGTGT ATGGGATGAG 
GATGATGACA TCAAGTCAAG AGTAAAGCAA CGGCTAAAGC GAGAGTCCGA TGATTTCCTT 
GGCCAAACCA TTATTGAGGT TCGGACACTG AGTGGCGAGA TGGACGTCTG GTACAACTTG 
GAGAAGAGGA CAGATAAATC AGCTGTCTCA GGGGCTATCC GACTGCAAAT CAGTGTGGAG 
ATCAAGGGAG AGGAGAAAGT AGCGCCATAT CATGTGCAGT ATACGTGTCT CCATGAGAAC 
CTTTTCCATT ACCTCACAGA CATTCAGGGT AGTGGAGGAG TCCTGATCCC TGAGGCTCGC 
GGAGACGATG CATGGAAGGT GTACTTTGAT GAGACTGCCC AAGAAATTGT GGATGAATTT 
GCCATGCGCT ATGGCATCGA GTCCATATAT CAGGCCATGA CGCACTTTGC ATGTTTGTCA 
TCCAAGTACA TGTGTCCTGG TGTGCCTGCG GTGATGAGCA CCTTATTGGC CAACATCAAT 
GCCTACTATG CCCATACAAC TGCCTCTACC AACGTCTCTG CATCTGATCG CTTTGCAGCC 
TCCAACTTTG GGAAAGAGAG ATTTGTAAAA CTACTGGACC AGCTGCACAA CTCACTGAGG 
ATTGACCTCT CTACCTACAG GAATAATTTC CCTGCTGGAA GCCCTGAGCG GCTTCAGGAC 
TTAAAGTCAA CAGTGGATTT GCTGACCAGC ATTACTTTCT TCAGAATGAA GGTACAAGAA 
CTGCAGAGCC CTCCAAGAGC CAGCCAAGTG GTAAAGGATT GTGTGAAGGC CTGTTTGAAC 
TCCACATATG AGTATATCTT CAACAACTGC CATGACTTGT ATAGTCGCCA GTACCAGCTG 
AAACAGGAGC TACCTCCAGA GGAACAAGGA CCCAGCATTC GGAACCTGGA TTTTTGGCCC 
AAACTCATCA CGCTCATTGT GTCAATCATA GAGGAAGATA AGAATTCCTA CACACTCGTT 
CTGAACCAGT TTCCTCAGGA ATTGAATGTG GGAAAAGTCA GCGCAGAAGT GATGTGGCAG 
CTCTTTGCCC AAGACATGAA ATATGCACTG GAGGAGCATG AGAAGGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATACGTGCGA 
GATCTGCCTG CCCTGCAGGG GCAGGTGCCT GAGTACCCAG TGTGGTTTGA GCAGTTTGTT 
CTGCAATGGC TGGATGAAAA TGAGGATGTG TCCCTGGAAT TCCTGCGTGG GGCTCTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CACTCTTTTC CTGCTCTGTG 
GTGGATGTCT TCACACAGCT CAACCAGAGC TTTGAGATCA TCCGGAAGCT GGAGTGCCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGG AGATTTGCTA AGACCATCGG GAAGGTGTTG 
ATGCAGTATG CAAACATCTT ATCAAAGAGC TTCCCAGATT ATTGCACAAA GGAGAAAATG 
CCCTGCATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAGGAGTT GGACCCTGAA GCTGCAGACA GTTTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTCAGC ATGGTATTTG GAAACAGTTT TCAGGTGCGG 
ATTGATGAGT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AGGTGCGAGG CCCAGGGAAT 
GCATCCCCCA ACGCCAGGGC CTCTGTGGCT CAGGATGCAG ATAGTGTGCT CCGGCCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACACTC TTTGCCACTG TGTGTGAGAA GACAGTCCTG 
AAGCGTGTAC TGAAGGAGCT CTGGAGGGTG GTCATGAACA CAATGGAAAG AATGATTGTT 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TTACTGCTGC CAAGGAGCTG 
AGCCATCTTT CTAAGCTCAA GGACCACATG GTGCGAGAGG AAACACGGAG TCTCACTCCA 
AAGCAGTGTG AAGTCCTTGA CCTCGCTCTG GACACTATCA AGCAATACTT TCATGCAGGA 
GGTAATGGGC TGAAGAAAAC CTTCCTGGAG AAGAGCTCAG ATCTGCAGTC TCTACGCTAT 
GCCCTGTCTC TCTACACACA GACCACAGAT ACACTCATCA AAACCTTTGT ACGCTCACAG 
ACTGCCCAGG TGCATGATGG GAAAGGTATT AGGTTTACTG CTAATGAGGA CATTCGGCCG 
GAAAAGGGAT CTGGTGTGGA TGATCCTGTG GGAGAAGTCT CTATTCAGGT GGACTTGTTT 
ACACATCCTG GAACTGGGGA GCACAAGGTC ACAGTGAAAG TGGTAGCTGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCC TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGAAA AGAAGAGGAA GTTCACAACC AAGTCAAAAA GCAACAACTG GGCTCCCAAG 
TACAATGAGA CATTTCACTT CCTCCTGGGA AATGAAGAAG GGCCAGAAGC CTATGAATTG 
CAAATATGTG TGAAGGATTA CTGCTTTGCC CGTGAGGATC GTGTTTTGGG GCTGGCTGTG 
ATGCCTCTGA GGGATGTAGC AGCCAAGGGC AGCTGTGCTT GCTGGTGTCC CTTGGGCCGG 
AAGATCCACA TGGATGAGAC AGGCATGACT ATTCTCCGGA TTCTATCTCA AAGGAGCAAT 
GACGAAGTGG CTCGAGAGTT TGTGAAGCTC AAATCGGAGT CTCGTTCCAT GGAAGAGGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117837.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973312.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe39193
Clone ID Related Accession (Same CDS sequence)	XM_014117837.2 , XM_014117837.1
Accession Version	XM_014117837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCATGCC ACCTCTTCCT CCTGTACGTC CATCTGTCCC 
TCTGTCCTTG TATCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973312.1
CDS	885..5045
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNHATSSSCTSICPSVLVSV

Target ORF information:

RefSeq Version	XM_014117837.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117837.2

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCATGCC ACCTCTTCCT CCTGTACGTC CATCTGTCCC 
TCTGTCCTTG TATCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117837.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973312.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X13, mRNA.

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CloneID	OCe39193
Clone ID Related Accession (Same CDS sequence)	XM_014117837.2 , XM_014117837.1
Accession Version	XM_014117837.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCATGCC ACCTCTTCCT CCTGTACGTC CATCTGTCCC 
TCTGTCCTTG TATCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973312.1
CDS	875..5035
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNHATSSSCTSICPSVLVSV

Target ORF information:

RefSeq Version	XM_014117837.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117837.1

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCATGCC ACCTCTTCCT CCTGTACGTC CATCTGTCCC 
TCTGTCCTTG TATCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117838.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973313.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe39194
Clone ID Related Accession (Same CDS sequence)	XM_014117838.1
Accession Version	XM_014117838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAAGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973313.1
CDS	875..4987
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQA

Target ORF information:

RefSeq Version	XM_014117838.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117838.1

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAAGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_022425547.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281255.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe14576
Clone ID Related Accession (Same CDS sequence)	XM_022425547.1
Accession Version	XM_022425547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT 
GATAGTGCTG TTGATGACCG GGATAGTGAC TATCGCAGTG AGACCAGCAA TAGCATCCCA 
CCTCCTTATC ATACAACTTC CCAGCCCAAC GCTTCTGTGC ACCAGTTTCC TGTGCCCATG 
CGATTGCCAC AGCAGCTGCT ACTTCAGGGC AATTCCCGGG ATTCTTGCAA TGACTCCATG 
CAAAGTTACG ATCTTGATTA TCCAGAGCGG CGGGCCCTTA GCCCCACCAG CAGTAGTAGG 
TATGGCTCAT CCTGTAATGT GAGTCAAGGA AGCTCTCAGT TGAGTGAACT AGACCAGTGT 
CACGAACAAG ATGATGACCG TCGGGAGAGG GATTCAATTC ATTCATGCCA CAGCTCTGGC 
AGCTTCTCCA GAGACGGCCA GGTGGGCTTT GGAGAACAAG AGAAAGCCTT GGAGGTAACA 
GGTGAAGAAG AGAAGGGGAG AGTGTGTGAA CCCAAGGACA AGAAAAAAGA TACCACAACC 
CATCTTCCCC CAGATCTGGT GCTACCCAAA GACCACGTCC TAGGCCCCCA GGAGAGTTTT 
CCTGAGGAGA ATGGTTCTTC ACCATTTACC CAAGCAAGAG CTCACTGGAT CCGAGCAGTT 
ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCGGATGATG GTCACCCTTC TTTGCCCCAG 
TGGCTTCCGG AAGGGCCTGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGC 
AGAAAGAAAC CATTGCCACT TGTCAGTGAT CTGGCTATGT CATTGGTCCA GTCCCGGAAA 
GCAGGAATTA CTTCTGCAAT GGCTACACGT ACCTCTCTGA AGGATGAAGA GCTGAAATCC 
CATGTGTATA AGAAAACCCT GCAGGCCTTA ATCTACCCAA TCTCGTGCAC CACACCCCAC 
AACTTTGAAG TCTGGACAGC CACTACCCCA ACCTACTGCT ATGAGTGTGA AGGTCTGCTC 
TGGGGCATTG CCCGGCAGGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG 
TGCCAAGACC TGCTCAACGC CGACTGCCTG CAGCGGGCTG CAGAAAAGAG TTCTAAACAT 
GGAGCTGAGG ACCGGACCCA GAACATAATC ATGGCGATGA AGGACCGCAT GAAGATCCGA 
GAGCGGAATA AGCCAGAGAT CTTTGAGGTT ATCCGAGATG TCTTTACCGT GAGCAAAGTT 
GCCCATGTGC AACAGATGAA AACAGTGAAG CAGAGTGTAC TGGATGGCAC CTCCAAATGG 
TCAGCCAAAA TTACCATCAC TGTGGTGTGT GCCCAGGGCC TACAAGCCAA GGACAAGACA 
GGATCCAGTG ACCCTTATGT GACTGTGCAA GTGGGCAAAA CCAAGAAGCG TACCAAGACT 
ATTTTTGGGA ACCTGAATCC TGTTTGGGAA GAGAAGTTCC ATTTTGAGTG CCACAACTCT 
TCTGACCGCA TTAAGGTACG TGTATGGGAT GAGGATGATG ACATCAAGTC AAGAGTAAAG 
CAACGGCTAA AGCGAGAGTC CGATGATTTC CTTGGCCAAA CCATTATTGA GGTTCGGACA 
CTGAGTGGCG AGATGGACGT CTGGTACAAC TTGGAGAAGA GGACAGATAA ATCAGCTGTC 
TCAGGGGCTA TCCGACTGCA AATCAGTGTG GAGATCAAGG GAGAGGAGAA AGTAGCGCCA 
TATCATGTGC AGTATACGTG TCTCCATGAG AACCTTTTCC ATTACCTCAC AGACATTCAG 
GGTAGTGGAG GAGTCCTGAT CCCTGAGGCT CGCGGAGACG ATGCATGGAA GGTGTACTTT 
GATGAGACTG CCCAAGAAAT TGTGGATGAA TTTGCCATGC GCTATGGCAT CGAGTCCATA 
TATCAGGCCA TGACGCACTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTGCCT 
GCGGTGATGA GCACCTTATT GGCCAACATC AATGCCTACT ATGCCCATAC AACTGCCTCT 
ACCAACGTCT CTGCATCTGA TCGCTTTGCA GCCTCCAACT TTGGGAAAGA GAGATTTGTA 
AAACTACTGG ACCAGCTGCA CAACTCACTG AGGATTGACC TCTCTACCTA CAGGAATAAT 
TTCCCTGCTG GAAGCCCTGA GCGGCTTCAG GACTTAAAGT CAACAGTGGA TTTGCTGACC 
AGCATTACTT TCTTCAGAAT GAAGGTACAA GAACTGCAGA GCCCTCCAAG AGCCAGCCAA 
GTGGTAAAGG ATTGTGTGAA GGCCTGTTTG AACTCCACAT ATGAGTATAT CTTCAACAAC 
TGCCATGACT TGTATAGTCG CCAGTACCAG CTGAAACAGG AGCTACCTCC AGAGGAACAA 
GGACCCAGCA TTCGGAACCT GGATTTTTGG CCCAAACTCA TCACGCTCAT TGTGTCAATC 
ATAGAGGAAG ATAAGAATTC CTACACACTC GTTCTGAACC AGTTTCCTCA GGAATTGAAT 
GTGGGAAAAG TCAGCGCAGA AGTGATGTGG CAGCTCTTTG CCCAAGACAT GAAATATGCA 
CTGGAGGAGC ATGAGAAGGA CCGGCTGTGT AAGAGTGCTG ACTACATGAA CCTGCACTTC 
AAGGTGAAGT GGCTCCACAA TGAATACGTG CGAGATCTGC CTGCCCTGCA GGGGCAGGTG 
CCTGAGTACC CAGTGTGGTT TGAGCAGTTT GTTCTGCAAT GGCTGGATGA AAATGAGGAT 
GTGTCCCTGG AATTCCTGCG TGGGGCTCTG GAACGAGATA AGAAGGATGG GTTCCAGCAG 
ACATCAGAGC ATGCACTCTT TTCCTGCTCT GTGGTGGATG TCTTCACACA GCTCAACCAG 
AGCTTTGAGA TCATCCGGAA GCTGGAGTGC CCAGACCCCA ACATCCTTGC CCACTACATG 
AGGAGATTTG CTAAGACCAT CGGGAAGGTG TTGATGCAGT ATGCAAACAT CTTATCAAAG 
AGCTTCCCAG ATTATTGCAC AAAGGAGAAA ATGCCCTGCA TCCTGATGAA CAACATGCAG 
CAACTGAGGG TCCAGCTGGA GAAAATGTTT GAGGCCATGG GAGGCAAGGA GTTGGACCCT 
GAAGCTGCAG ACAGTTTGAA GGAGCTGCAG GTGAAACTGA ATACAGTTCT GGATGAGCTC 
AGCATGGTAT TTGGAAACAG TTTTCAGGTG CGGATTGATG AGTGTGTTCG ACAAATGGCT 
GACATCCTGG GCCAGGTGCG AGGCCCAGGG AATGCATCCC CCAACGCCAG GGCCTCTGTG 
GCTCAGGATG CAGATAGTGT GCTCCGGCCT CTCATGGACT TCCTGGATGG CAACCTCACA 
CTCTTTGCCA CTGTGTGTGA GAAGACAGTC CTGAAGCGTG TACTGAAGGA GCTCTGGAGG 
GTGGTCATGA ACACAATGGA AAGAATGATT GTTCTGCCCC CACTCACTGA CCAGACGGGC 
ACCCAGCTGA TCTTTACTGC TGCCAAGGAG CTGAGCCATC TTTCTAAGCT CAAGGACCAC 
ATGGTGCGAG AGGAAACACG GAGTCTCACT CCAAAGCAGT GTGAAGTCCT TGACCTCGCT 
CTGGACACTA TCAAGCAATA CTTTCATGCA GGAGGTAATG GGCTGAAGAA AACCTTCCTG 
GAGAAGAGCT CAGATCTGCA GTCTCTACGC TATGCCCTGT CTCTCTACAC ACAGACCACA 
GATACACTCA TCAAAACCTT TGTACGCTCA CAGACTGCCC AGGTGCATGA TGGGAAAGGT 
ATTAGGTTTA CTGCTAATGA GGACATTCGG CCGGAAAAGG GATCTGGTGT GGATGATCCT 
GTGGGAGAAG TCTCTATTCA GGTGGACTTG TTTACACATC CTGGAACTGG GGAGCACAAG 
GTCACAGTGA AAGTGGTAGC TGCCAATGAC CTCAAGTGGC AGACAGCGGG TATGTTCCGG 
CCCTTTGTGG AAGTGACCAT GGTTGGCCCA CACCAAAGTG AAAAGAAGAG GAAGTTCACA 
ACCAAGTCAA AAAGCAACAA CTGGGCTCCC AAGTACAATG AGACATTTCA CTTCCTCCTG 
GGAAATGAAG AAGGGCCAGA AGCCTATGAA TTGCAAATAT GTGTGAAGGA TTACTGCTTT 
GCCCGTGAGG ATCGTGTTTT GGGGCTGGCT GTGATGCCTC TGAGGGATGT AGCAGCCAAG 
GGCAGCTGTG CTTGCTGGTG TCCCTTGGGC CGGAAGATCC ACATGGATGA GACAGGCATG 
ACTATTCTCC GGATTCTATC TCAAAGGAGC AATGACGAAG TGGCTCGAGA GTTTGTGAAG 
CTCAAATCGG AGTCTCGTTC CATGGAAGAG GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281255.1
CDS	335..5173
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425547.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425547.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT 
GATAGTGCTG TTGATGACCG GGATAGTGAC TATCGCAGTG AGACCAGCAA TAGCATCCCA 
CCTCCTTATC ATACAACTTC CCAGCCCAAC GCTTCTGTGC ACCAGTTTCC TGTGCCCATG 
CGATTGCCAC AGCAGCTGCT ACTTCAGGGC AATTCCCGGG ATTCTTGCAA TGACTCCATG 
CAAAGTTACG ATCTTGATTA TCCAGAGCGG CGGGCCCTTA GCCCCACCAG CAGTAGTAGG 
TATGGCTCAT CCTGTAATGT GAGTCAAGGA AGCTCTCAGT TGAGTGAACT AGACCAGTGT 
CACGAACAAG ATGATGACCG TCGGGAGAGG GATTCAATTC ATTCATGCCA CAGCTCTGGC 
AGCTTCTCCA GAGACGGCCA GGTGGGCTTT GGAGAACAAG AGAAAGCCTT GGAGGTAACA 
GGTGAAGAAG AGAAGGGGAG AGTGTGTGAA CCCAAGGACA AGAAAAAAGA TACCACAACC 
CATCTTCCCC CAGATCTGGT GCTACCCAAA GACCACGTCC TAGGCCCCCA GGAGAGTTTT 
CCTGAGGAGA ATGGTTCTTC ACCATTTACC CAAGCAAGAG CTCACTGGAT CCGAGCAGTT 
ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCGGATGATG GTCACCCTTC TTTGCCCCAG 
TGGCTTCCGG AAGGGCCTGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGC 
AGAAAGAAAC CATTGCCACT TGTCAGTGAT CTGGCTATGT CATTGGTCCA GTCCCGGAAA 
GCAGGAATTA CTTCTGCAAT GGCTACACGT ACCTCTCTGA AGGATGAAGA GCTGAAATCC 
CATGTGTATA AGAAAACCCT GCAGGCCTTA ATCTACCCAA TCTCGTGCAC CACACCCCAC 
AACTTTGAAG TCTGGACAGC CACTACCCCA ACCTACTGCT ATGAGTGTGA AGGTCTGCTC 
TGGGGCATTG CCCGGCAGGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG 
TGCCAAGACC TGCTCAACGC CGACTGCCTG CAGCGGGCTG CAGAAAAGAG TTCTAAACAT 
GGAGCTGAGG ACCGGACCCA GAACATAATC ATGGCGATGA AGGACCGCAT GAAGATCCGA 
GAGCGGAATA AGCCAGAGAT CTTTGAGGTT ATCCGAGATG TCTTTACCGT GAGCAAAGTT 
GCCCATGTGC AACAGATGAA AACAGTGAAG CAGAGTGTAC TGGATGGCAC CTCCAAATGG 
TCAGCCAAAA TTACCATCAC TGTGGTGTGT GCCCAGGGCC TACAAGCCAA GGACAAGACA 
GGATCCAGTG ACCCTTATGT GACTGTGCAA GTGGGCAAAA CCAAGAAGCG TACCAAGACT 
ATTTTTGGGA ACCTGAATCC TGTTTGGGAA GAGAAGTTCC ATTTTGAGTG CCACAACTCT 
TCTGACCGCA TTAAGGTACG TGTATGGGAT GAGGATGATG ACATCAAGTC AAGAGTAAAG 
CAACGGCTAA AGCGAGAGTC CGATGATTTC CTTGGCCAAA CCATTATTGA GGTTCGGACA 
CTGAGTGGCG AGATGGACGT CTGGTACAAC TTGGAGAAGA GGACAGATAA ATCAGCTGTC 
TCAGGGGCTA TCCGACTGCA AATCAGTGTG GAGATCAAGG GAGAGGAGAA AGTAGCGCCA 
TATCATGTGC AGTATACGTG TCTCCATGAG AACCTTTTCC ATTACCTCAC AGACATTCAG 
GGTAGTGGAG GAGTCCTGAT CCCTGAGGCT CGCGGAGACG ATGCATGGAA GGTGTACTTT 
GATGAGACTG CCCAAGAAAT TGTGGATGAA TTTGCCATGC GCTATGGCAT CGAGTCCATA 
TATCAGGCCA TGACGCACTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTGCCT 
GCGGTGATGA GCACCTTATT GGCCAACATC AATGCCTACT ATGCCCATAC AACTGCCTCT 
ACCAACGTCT CTGCATCTGA TCGCTTTGCA GCCTCCAACT TTGGGAAAGA GAGATTTGTA 
AAACTACTGG ACCAGCTGCA CAACTCACTG AGGATTGACC TCTCTACCTA CAGGAATAAT 
TTCCCTGCTG GAAGCCCTGA GCGGCTTCAG GACTTAAAGT CAACAGTGGA TTTGCTGACC 
AGCATTACTT TCTTCAGAAT GAAGGTACAA GAACTGCAGA GCCCTCCAAG AGCCAGCCAA 
GTGGTAAAGG ATTGTGTGAA GGCCTGTTTG AACTCCACAT ATGAGTATAT CTTCAACAAC 
TGCCATGACT TGTATAGTCG CCAGTACCAG CTGAAACAGG AGCTACCTCC AGAGGAACAA 
GGACCCAGCA TTCGGAACCT GGATTTTTGG CCCAAACTCA TCACGCTCAT TGTGTCAATC 
ATAGAGGAAG ATAAGAATTC CTACACACTC GTTCTGAACC AGTTTCCTCA GGAATTGAAT 
GTGGGAAAAG TCAGCGCAGA AGTGATGTGG CAGCTCTTTG CCCAAGACAT GAAATATGCA 
CTGGAGGAGC ATGAGAAGGA CCGGCTGTGT AAGAGTGCTG ACTACATGAA CCTGCACTTC 
AAGGTGAAGT GGCTCCACAA TGAATACGTG CGAGATCTGC CTGCCCTGCA GGGGCAGGTG 
CCTGAGTACC CAGTGTGGTT TGAGCAGTTT GTTCTGCAAT GGCTGGATGA AAATGAGGAT 
GTGTCCCTGG AATTCCTGCG TGGGGCTCTG GAACGAGATA AGAAGGATGG GTTCCAGCAG 
ACATCAGAGC ATGCACTCTT TTCCTGCTCT GTGGTGGATG TCTTCACACA GCTCAACCAG 
AGCTTTGAGA TCATCCGGAA GCTGGAGTGC CCAGACCCCA ACATCCTTGC CCACTACATG 
AGGAGATTTG CTAAGACCAT CGGGAAGGTG TTGATGCAGT ATGCAAACAT CTTATCAAAG 
AGCTTCCCAG ATTATTGCAC AAAGGAGAAA ATGCCCTGCA TCCTGATGAA CAACATGCAG 
CAACTGAGGG TCCAGCTGGA GAAAATGTTT GAGGCCATGG GAGGCAAGGA GTTGGACCCT 
GAAGCTGCAG ACAGTTTGAA GGAGCTGCAG GTGAAACTGA ATACAGTTCT GGATGAGCTC 
AGCATGGTAT TTGGAAACAG TTTTCAGGTG CGGATTGATG AGTGTGTTCG ACAAATGGCT 
GACATCCTGG GCCAGGTGCG AGGCCCAGGG AATGCATCCC CCAACGCCAG GGCCTCTGTG 
GCTCAGGATG CAGATAGTGT GCTCCGGCCT CTCATGGACT TCCTGGATGG CAACCTCACA 
CTCTTTGCCA CTGTGTGTGA GAAGACAGTC CTGAAGCGTG TACTGAAGGA GCTCTGGAGG 
GTGGTCATGA ACACAATGGA AAGAATGATT GTTCTGCCCC CACTCACTGA CCAGACGGGC 
ACCCAGCTGA TCTTTACTGC TGCCAAGGAG CTGAGCCATC TTTCTAAGCT CAAGGACCAC 
ATGGTGCGAG AGGAAACACG GAGTCTCACT CCAAAGCAGT GTGAAGTCCT TGACCTCGCT 
CTGGACACTA TCAAGCAATA CTTTCATGCA GGAGGTAATG GGCTGAAGAA AACCTTCCTG 
GAGAAGAGCT CAGATCTGCA GTCTCTACGC TATGCCCTGT CTCTCTACAC ACAGACCACA 
GATACACTCA TCAAAACCTT TGTACGCTCA CAGACTGCCC AGGTGCATGA TGGGAAAGGT 
ATTAGGTTTA CTGCTAATGA GGACATTCGG CCGGAAAAGG GATCTGGTGT GGATGATCCT 
GTGGGAGAAG TCTCTATTCA GGTGGACTTG TTTACACATC CTGGAACTGG GGAGCACAAG 
GTCACAGTGA AAGTGGTAGC TGCCAATGAC CTCAAGTGGC AGACAGCGGG TATGTTCCGG 
CCCTTTGTGG AAGTGACCAT GGTTGGCCCA CACCAAAGTG AAAAGAAGAG GAAGTTCACA 
ACCAAGTCAA AAAGCAACAA CTGGGCTCCC AAGTACAATG AGACATTTCA CTTCCTCCTG 
GGAAATGAAG AAGGGCCAGA AGCCTATGAA TTGCAAATAT GTGTGAAGGA TTACTGCTTT 
GCCCGTGAGG ATCGTGTTTT GGGGCTGGCT GTGATGCCTC TGAGGGATGT AGCAGCCAAG 
GGCAGCTGTG CTTGCTGGTG TCCCTTGGGC CGGAAGATCC ACATGGATGA GACAGGCATG 
ACTATTCTCC GGATTCTATC TCAAAGGAGC AATGACGAAG TGGCTCGAGA GTTTGTGAAG 
CTCAAATCGG AGTCTCGTTC CATGGAAGAG GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_022425548.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281256.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X7, mRNA.

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CloneID	OCe14577
Clone ID Related Accession (Same CDS sequence)	XM_022425548.1
Accession Version	XM_022425548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT 
GATAGTGCTG TTGATGACCG GGATAGTGAC TATCGCAGTG AGACCAGCAA TAGCATCCCA 
CCTCCTTATC ATACAACTTC CCAGCCCAAC GCTTCTGTGC ACCAGTTTCC TGTGCCCATG 
CGATTGCCAC AGCAGCTGCT ACTTCAGGGC AATTCCCGGG ATTCTTGCAA TGACTCCATG 
CAAAGTTACG ATCTTGATTA TCCAGAGCGG CGGGCCCTTA GCCCCACCAG CAGTAGTAGG 
TATGGCTCAT CCTGTAATGT GAGTCAAGGA AGCTCTCAGT TGAGTGAACT AGACCAGTGT 
CACGAACAAG ATGATGACCG TCGGGAGAGG GATTCAATTC ATTCATGCCA CAGCTCTGGC 
AGCTTCTCCA GAGACGGCCA GGTGGGCTTT GGAGAACAAG AGAAAGCCTT GGAGGTAACA 
GGTGAAGAAG AGAAGGGGAG AGTGTGTGAA CCCAAGGACA AGAAAAAAGA TACCACAACC 
CATCTTCCCC CAGATCTGGT GCTACCCAAA GACCACGTCC TAGGCCCCCA GGAGAGTTTT 
CCTGAGGAGA ATGGTTCTTC ACCATTTACC CAAGCAAGAG CTCACTGGAT CCGAGCAGTT 
ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCGGATGATG GTCACCCTTC TTTGCCCCAG 
TGGCTTCCGG AAGGGCCTGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGC 
AGAAAGAAAC CATTGCCACT TGTCAGTGAT CTGTCATTGG TCCAGTCCCG GAAAGCAGGA 
ATTACTTCTG CAATGGCTAC ACGTACCTCT CTGAAGGATG AAGAGCTGAA ATCCCATGTG 
TATAAGAAAA CCCTGCAGGC CTTAATCTAC CCAATCTCGT GCACCACACC CCACAACTTT 
GAAGTCTGGA CAGCCACTAC CCCAACCTAC TGCTATGAGT GTGAAGGTCT GCTCTGGGGC 
ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAA 
GACCTGCTCA ACGCCGACTG CCTGCAGCGG GCTGCAGAAA AGAGTTCTAA ACATGGAGCT 
GAGGACCGGA CCCAGAACAT AATCATGGCG ATGAAGGACC GCATGAAGAT CCGAGAGCGG 
AATAAGCCAG AGATCTTTGA GGTTATCCGA GATGTCTTTA CCGTGAGCAA AGTTGCCCAT 
GTGCAACAGA TGAAAACAGT GAAGCAGAGT GTACTGGATG GCACCTCCAA ATGGTCAGCC 
AAAATTACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACTATTTTT 
GGGAACCTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC 
CGCATTAAGG TACGTGTATG GGATGAGGAT GATGACATCA AGTCAAGAGT AAAGCAACGG 
CTAAAGCGAG AGTCCGATGA TTTCCTTGGC CAAACCATTA TTGAGGTTCG GACACTGAGT 
GGCGAGATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ATAAATCAGC TGTCTCAGGG 
GCTATCCGAC TGCAAATCAG TGTGGAGATC AAGGGAGAGG AGAAAGTAGC GCCATATCAT 
GTGCAGTATA CGTGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGTAGT 
GGAGGAGTCC TGATCCCTGA GGCTCGCGGA GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACTGCCCAAG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATCGAGTC CATATATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCTGCGGTG 
ATGAGCACCT TATTGGCCAA CATCAATGCC TACTATGCCC ATACAACTGC CTCTACCAAC 
GTCTCTGCAT CTGATCGCTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTAAAACTA 
CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT 
GCTGGAAGCC CTGAGCGGCT TCAGGACTTA AAGTCAACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GAATGAAGGT ACAAGAACTG CAGAGCCCTC CAAGAGCCAG CCAAGTGGTA 
AAGGATTGTG TGAAGGCCTG TTTGAACTCC ACATATGAGT ATATCTTCAA CAACTGCCAT 
GACTTGTATA GTCGCCAGTA CCAGCTGAAA CAGGAGCTAC CTCCAGAGGA ACAAGGACCC 
AGCATTCGGA ACCTGGATTT TTGGCCCAAA CTCATCACGC TCATTGTGTC AATCATAGAG 
GAAGATAAGA ATTCCTACAC ACTCGTTCTG AACCAGTTTC CTCAGGAATT GAATGTGGGA 
AAAGTCAGCG CAGAAGTGAT GTGGCAGCTC TTTGCCCAAG ACATGAAATA TGCACTGGAG 
GAGCATGAGA AGGACCGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG 
AAGTGGCTCC ACAATGAATA CGTGCGAGAT CTGCCTGCCC TGCAGGGGCA GGTGCCTGAG 
TACCCAGTGT GGTTTGAGCA GTTTGTTCTG CAATGGCTGG ATGAAAATGA GGATGTGTCC 
CTGGAATTCC TGCGTGGGGC TCTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA 
GAGCATGCAC TCTTTTCCTG CTCTGTGGTG GATGTCTTCA CACAGCTCAA CCAGAGCTTT 
GAGATCATCC GGAAGCTGGA GTGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA 
TTTGCTAAGA CCATCGGGAA GGTGTTGATG CAGTATGCAA ACATCTTATC AAAGAGCTTC 
CCAGATTATT GCACAAAGGA GAAAATGCCC TGCATCCTGA TGAACAACAT GCAGCAACTG 
AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC ATGGGAGGCA AGGAGTTGGA CCCTGAAGCT 
GCAGACAGTT TGAAGGAGCT GCAGGTGAAA CTGAATACAG TTCTGGATGA GCTCAGCATG 
GTATTTGGAA ACAGTTTTCA GGTGCGGATT GATGAGTGTG TTCGACAAAT GGCTGACATC 
CTGGGCCAGG TGCGAGGCCC AGGGAATGCA TCCCCCAACG CCAGGGCCTC TGTGGCTCAG 
GATGCAGATA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ATGGCAACCT CACACTCTTT 
GCCACTGTGT GTGAGAAGAC AGTCCTGAAG CGTGTACTGA AGGAGCTCTG GAGGGTGGTC 
ATGAACACAA TGGAAAGAAT GATTGTTCTG CCCCCACTCA CTGACCAGAC GGGCACCCAG 
CTGATCTTTA CTGCTGCCAA GGAGCTGAGC CATCTTTCTA AGCTCAAGGA CCACATGGTG 
CGAGAGGAAA CACGGAGTCT CACTCCAAAG CAGTGTGAAG TCCTTGACCT CGCTCTGGAC 
ACTATCAAGC AATACTTTCA TGCAGGAGGT AATGGGCTGA AGAAAACCTT CCTGGAGAAG 
AGCTCAGATC TGCAGTCTCT ACGCTATGCC CTGTCTCTCT ACACACAGAC CACAGATACA 
CTCATCAAAA CCTTTGTACG CTCACAGACT GCCCAGGTGC ATGATGGGAA AGGTATTAGG 
TTTACTGCTA ATGAGGACAT TCGGCCGGAA AAGGGATCTG GTGTGGATGA TCCTGTGGGA 
GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA 
GTGAAAGTGG TAGCTGCCAA TGACCTCAAG TGGCAGACAG CGGGTATGTT CCGGCCCTTT 
GTGGAAGTGA CCATGGTTGG CCCACACCAA AGTGAAAAGA AGAGGAAGTT CACAACCAAG 
TCAAAAAGCA ACAACTGGGC TCCCAAGTAC AATGAGACAT TTCACTTCCT CCTGGGAAAT 
GAAGAAGGGC CAGAAGCCTA TGAATTGCAA ATATGTGTGA AGGATTACTG CTTTGCCCGT 
GAGGATCGTG TTTTGGGGCT GGCTGTGATG CCTCTGAGGG ATGTAGCAGC CAAGGGCAGC 
TGTGCTTGCT GGTGTCCCTT GGGCCGGAAG ATCCACATGG ATGAGACAGG CATGACTATT 
CTCCGGATTC TATCTCAAAG GAGCAATGAC GAAGTGGCTC GAGAGTTTGT GAAGCTCAAA 
TCGGAGTCTC GTTCCATGGA AGAGGGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281256.1
CDS	335..5167
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425548.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425548.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT 
GATAGTGCTG TTGATGACCG GGATAGTGAC TATCGCAGTG AGACCAGCAA TAGCATCCCA 
CCTCCTTATC ATACAACTTC CCAGCCCAAC GCTTCTGTGC ACCAGTTTCC TGTGCCCATG 
CGATTGCCAC AGCAGCTGCT ACTTCAGGGC AATTCCCGGG ATTCTTGCAA TGACTCCATG 
CAAAGTTACG ATCTTGATTA TCCAGAGCGG CGGGCCCTTA GCCCCACCAG CAGTAGTAGG 
TATGGCTCAT CCTGTAATGT GAGTCAAGGA AGCTCTCAGT TGAGTGAACT AGACCAGTGT 
CACGAACAAG ATGATGACCG TCGGGAGAGG GATTCAATTC ATTCATGCCA CAGCTCTGGC 
AGCTTCTCCA GAGACGGCCA GGTGGGCTTT GGAGAACAAG AGAAAGCCTT GGAGGTAACA 
GGTGAAGAAG AGAAGGGGAG AGTGTGTGAA CCCAAGGACA AGAAAAAAGA TACCACAACC 
CATCTTCCCC CAGATCTGGT GCTACCCAAA GACCACGTCC TAGGCCCCCA GGAGAGTTTT 
CCTGAGGAGA ATGGTTCTTC ACCATTTACC CAAGCAAGAG CTCACTGGAT CCGAGCAGTT 
ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCGGATGATG GTCACCCTTC TTTGCCCCAG 
TGGCTTCCGG AAGGGCCTGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGC 
AGAAAGAAAC CATTGCCACT TGTCAGTGAT CTGTCATTGG TCCAGTCCCG GAAAGCAGGA 
ATTACTTCTG CAATGGCTAC ACGTACCTCT CTGAAGGATG AAGAGCTGAA ATCCCATGTG 
TATAAGAAAA CCCTGCAGGC CTTAATCTAC CCAATCTCGT GCACCACACC CCACAACTTT 
GAAGTCTGGA CAGCCACTAC CCCAACCTAC TGCTATGAGT GTGAAGGTCT GCTCTGGGGC 
ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAA 
GACCTGCTCA ACGCCGACTG CCTGCAGCGG GCTGCAGAAA AGAGTTCTAA ACATGGAGCT 
GAGGACCGGA CCCAGAACAT AATCATGGCG ATGAAGGACC GCATGAAGAT CCGAGAGCGG 
AATAAGCCAG AGATCTTTGA GGTTATCCGA GATGTCTTTA CCGTGAGCAA AGTTGCCCAT 
GTGCAACAGA TGAAAACAGT GAAGCAGAGT GTACTGGATG GCACCTCCAA ATGGTCAGCC 
AAAATTACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACTATTTTT 
GGGAACCTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC 
CGCATTAAGG TACGTGTATG GGATGAGGAT GATGACATCA AGTCAAGAGT AAAGCAACGG 
CTAAAGCGAG AGTCCGATGA TTTCCTTGGC CAAACCATTA TTGAGGTTCG GACACTGAGT 
GGCGAGATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ATAAATCAGC TGTCTCAGGG 
GCTATCCGAC TGCAAATCAG TGTGGAGATC AAGGGAGAGG AGAAAGTAGC GCCATATCAT 
GTGCAGTATA CGTGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGTAGT 
GGAGGAGTCC TGATCCCTGA GGCTCGCGGA GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACTGCCCAAG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATCGAGTC CATATATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCTGCGGTG 
ATGAGCACCT TATTGGCCAA CATCAATGCC TACTATGCCC ATACAACTGC CTCTACCAAC 
GTCTCTGCAT CTGATCGCTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTAAAACTA 
CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT 
GCTGGAAGCC CTGAGCGGCT TCAGGACTTA AAGTCAACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GAATGAAGGT ACAAGAACTG CAGAGCCCTC CAAGAGCCAG CCAAGTGGTA 
AAGGATTGTG TGAAGGCCTG TTTGAACTCC ACATATGAGT ATATCTTCAA CAACTGCCAT 
GACTTGTATA GTCGCCAGTA CCAGCTGAAA CAGGAGCTAC CTCCAGAGGA ACAAGGACCC 
AGCATTCGGA ACCTGGATTT TTGGCCCAAA CTCATCACGC TCATTGTGTC AATCATAGAG 
GAAGATAAGA ATTCCTACAC ACTCGTTCTG AACCAGTTTC CTCAGGAATT GAATGTGGGA 
AAAGTCAGCG CAGAAGTGAT GTGGCAGCTC TTTGCCCAAG ACATGAAATA TGCACTGGAG 
GAGCATGAGA AGGACCGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG 
AAGTGGCTCC ACAATGAATA CGTGCGAGAT CTGCCTGCCC TGCAGGGGCA GGTGCCTGAG 
TACCCAGTGT GGTTTGAGCA GTTTGTTCTG CAATGGCTGG ATGAAAATGA GGATGTGTCC 
CTGGAATTCC TGCGTGGGGC TCTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA 
GAGCATGCAC TCTTTTCCTG CTCTGTGGTG GATGTCTTCA CACAGCTCAA CCAGAGCTTT 
GAGATCATCC GGAAGCTGGA GTGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA 
TTTGCTAAGA CCATCGGGAA GGTGTTGATG CAGTATGCAA ACATCTTATC AAAGAGCTTC 
CCAGATTATT GCACAAAGGA GAAAATGCCC TGCATCCTGA TGAACAACAT GCAGCAACTG 
AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC ATGGGAGGCA AGGAGTTGGA CCCTGAAGCT 
GCAGACAGTT TGAAGGAGCT GCAGGTGAAA CTGAATACAG TTCTGGATGA GCTCAGCATG 
GTATTTGGAA ACAGTTTTCA GGTGCGGATT GATGAGTGTG TTCGACAAAT GGCTGACATC 
CTGGGCCAGG TGCGAGGCCC AGGGAATGCA TCCCCCAACG CCAGGGCCTC TGTGGCTCAG 
GATGCAGATA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ATGGCAACCT CACACTCTTT 
GCCACTGTGT GTGAGAAGAC AGTCCTGAAG CGTGTACTGA AGGAGCTCTG GAGGGTGGTC 
ATGAACACAA TGGAAAGAAT GATTGTTCTG CCCCCACTCA CTGACCAGAC GGGCACCCAG 
CTGATCTTTA CTGCTGCCAA GGAGCTGAGC CATCTTTCTA AGCTCAAGGA CCACATGGTG 
CGAGAGGAAA CACGGAGTCT CACTCCAAAG CAGTGTGAAG TCCTTGACCT CGCTCTGGAC 
ACTATCAAGC AATACTTTCA TGCAGGAGGT AATGGGCTGA AGAAAACCTT CCTGGAGAAG 
AGCTCAGATC TGCAGTCTCT ACGCTATGCC CTGTCTCTCT ACACACAGAC CACAGATACA 
CTCATCAAAA CCTTTGTACG CTCACAGACT GCCCAGGTGC ATGATGGGAA AGGTATTAGG 
TTTACTGCTA ATGAGGACAT TCGGCCGGAA AAGGGATCTG GTGTGGATGA TCCTGTGGGA 
GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA 
GTGAAAGTGG TAGCTGCCAA TGACCTCAAG TGGCAGACAG CGGGTATGTT CCGGCCCTTT 
GTGGAAGTGA CCATGGTTGG CCCACACCAA AGTGAAAAGA AGAGGAAGTT CACAACCAAG 
TCAAAAAGCA ACAACTGGGC TCCCAAGTAC AATGAGACAT TTCACTTCCT CCTGGGAAAT 
GAAGAAGGGC CAGAAGCCTA TGAATTGCAA ATATGTGTGA AGGATTACTG CTTTGCCCGT 
GAGGATCGTG TTTTGGGGCT GGCTGTGATG CCTCTGAGGG ATGTAGCAGC CAAGGGCAGC 
TGTGCTTGCT GGTGTCCCTT GGGCCGGAAG ATCCACATGG ATGAGACAGG CATGACTATT 
CTCCGGATTC TATCTCAAAG GAGCAATGAC GAAGTGGCTC GAGAGTTTGT GAAGCTCAAA 
TCGGAGTCTC GTTCCATGGA AGAGGGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_022425549.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281257.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X8, mRNA.

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CloneID	OCe14578
Clone ID Related Accession (Same CDS sequence)	XM_022425549.1
Accession Version	XM_022425549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCAGCA GACTTTTGAA GATCCTGATA GTGCTGTTGA TGACCGGGAT 
AGTGACTATC GCAGTGAGAC CAGCAATAGC ATCCCACCTC CTTATCATAC AACTTCCCAG 
CCCAACGCTT CTGTGCACCA GTTTCCTGTG CCCATGCGAT TGCCACAGCA GCTGCTACTT 
CAGGGCAATT CCCGGGATTC TTGCAATGAC TCCATGCAAA GTTACGATCT TGATTATCCA 
GAGCGGCGGG CCCTTAGCCC CACCAGCAGT AGTAGGTATG GCTCATCCTG TAATGTGAGT 
CAAGGAAGCT CTCAGTTGAG TGAACTAGAC CAGTGTCACG AACAAGATGA TGACCGTCGG 
GAGAGGGATT CAATTCATTC ATGCCACAGC TCTGGCAGCT TCTCCAGAGA CGGCCAGGTG 
GGCTTTGGAG AACAAGAGAA AGCCTTGGAG GTAACAGGTG AAGAAGAGAA GGGGAGAGTG 
TGTGAACCCA AGGACAAGAA AAAAGATACC ACAACCCATC TTCCCCCAGA TCTGGTGCTA 
CCCAAAGACC ACGTCCTAGG CCCCCAGGAG AGTTTTCCTG AGGAGAATGG TTCTTCACCA 
TTTACCCAAG CAAGAGCTCA CTGGATCCGA GCAGTTACCA AGGTTCGACT CCAGCTGCAG 
GAGATTCCGG ATGATGGTCA CCCTTCTTTG CCCCAGTGGC TTCCGGAAGG GCCTGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGCAGAA AGAAACCATT GCCACTTGTC 
AGTGATCTGT CATTGGTCCA GTCCCGGAAA GCAGGAATTA CTTCTGCAAT GGCTACACGT 
ACCTCTCTGA AGGATGAAGA GCTGAAATCC CATGTGTATA AGAAAACCCT GCAGGCCTTA 
ATCTACCCAA TCTCGTGCAC CACACCCCAC AACTTTGAAG TCTGGACAGC CACTACCCCA 
ACCTACTGCT ATGAGTGTGA AGGTCTGCTC TGGGGCATTG CCCGGCAGGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAAGACC TGCTCAACGC CGACTGCCTG 
CAGCGGGCTG CAGAAAAGAG TTCTAAACAT GGAGCTGAGG ACCGGACCCA GAACATAATC 
ATGGCGATGA AGGACCGCAT GAAGATCCGA GAGCGGAATA AGCCAGAGAT CTTTGAGGTT 
ATCCGAGATG TCTTTACCGT GAGCAAAGTT GCCCATGTGC AACAGATGAA AACAGTGAAG 
CAGAGTGTAC TGGATGGCAC CTCCAAATGG TCAGCCAAAA TTACCATCAC TGTGGTGTGT 
GCCCAGGGCC TACAAGCCAA GGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTGGGCAAAA CCAAGAAGCG TACCAAGACT ATTTTTGGGA ACCTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGACCGCA TTAAGGTACG TGTATGGGAT 
GAGGATGATG ACATCAAGTC AAGAGTAAAG CAACGGCTAA AGCGAGAGTC CGATGATTTC 
CTTGGCCAAA CCATTATTGA GGTTCGGACA CTGAGTGGCG AGATGGACGT CTGGTACAAC 
TTGGAGAAGA GGACAGATAA ATCAGCTGTC TCAGGGGCTA TCCGACTGCA AATCAGTGTG 
GAGATCAAGG GAGAGGAGAA AGTAGCGCCA TATCATGTGC AGTATACGTG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGTAGTGGAG GAGTCCTGAT CCCTGAGGCT 
CGCGGAGACG ATGCATGGAA GGTGTACTTT GATGAGACTG CCCAAGAAAT TGTGGATGAA 
TTTGCCATGC GCTATGGCAT CGAGTCCATA TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTGCCT GCGGTGATGA GCACCTTATT GGCCAACATC 
AATGCCTACT ATGCCCATAC AACTGCCTCT ACCAACGTCT CTGCATCTGA TCGCTTTGCA 
GCCTCCAACT TTGGGAAAGA GAGATTTGTA AAACTACTGG ACCAGCTGCA CAACTCACTG 
AGGATTGACC TCTCTACCTA CAGGAATAAT TTCCCTGCTG GAAGCCCTGA GCGGCTTCAG 
GACTTAAAGT CAACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGAAT GAAGGTACAA 
GAACTGCAGA GCCCTCCAAG AGCCAGCCAA GTGGTAAAGG ATTGTGTGAA GGCCTGTTTG 
AACTCCACAT ATGAGTATAT CTTCAACAAC TGCCATGACT TGTATAGTCG CCAGTACCAG 
CTGAAACAGG AGCTACCTCC AGAGGAACAA GGACCCAGCA TTCGGAACCT GGATTTTTGG 
CCCAAACTCA TCACGCTCAT TGTGTCAATC ATAGAGGAAG ATAAGAATTC CTACACACTC 
GTTCTGAACC AGTTTCCTCA GGAATTGAAT GTGGGAAAAG TCAGCGCAGA AGTGATGTGG 
CAGCTCTTTG CCCAAGACAT GAAATATGCA CTGGAGGAGC ATGAGAAGGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATACGTG 
CGAGATCTGC CTGCCCTGCA GGGGCAGGTG CCTGAGTACC CAGTGTGGTT TGAGCAGTTT 
GTTCTGCAAT GGCTGGATGA AAATGAGGAT GTGTCCCTGG AATTCCTGCG TGGGGCTCTG 
GAACGAGATA AGAAGGATGG GTTCCAGCAG ACATCAGAGC ATGCACTCTT TTCCTGCTCT 
GTGGTGGATG TCTTCACACA GCTCAACCAG AGCTTTGAGA TCATCCGGAA GCTGGAGTGC 
CCAGACCCCA ACATCCTTGC CCACTACATG AGGAGATTTG CTAAGACCAT CGGGAAGGTG 
TTGATGCAGT ATGCAAACAT CTTATCAAAG AGCTTCCCAG ATTATTGCAC AAAGGAGAAA 
ATGCCCTGCA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAGGA GTTGGACCCT GAAGCTGCAG ACAGTTTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTC AGCATGGTAT TTGGAAACAG TTTTCAGGTG 
CGGATTGATG AGTGTGTTCG ACAAATGGCT GACATCCTGG GCCAGGTGCG AGGCCCAGGG 
AATGCATCCC CCAACGCCAG GGCCTCTGTG GCTCAGGATG CAGATAGTGT GCTCCGGCCT 
CTCATGGACT TCCTGGATGG CAACCTCACA CTCTTTGCCA CTGTGTGTGA GAAGACAGTC 
CTGAAGCGTG TACTGAAGGA GCTCTGGAGG GTGGTCATGA ACACAATGGA AAGAATGATT 
GTTCTGCCCC CACTCACTGA CCAGACGGGC ACCCAGCTGA TCTTTACTGC TGCCAAGGAG 
CTGAGCCATC TTTCTAAGCT CAAGGACCAC ATGGTGCGAG AGGAAACACG GAGTCTCACT 
CCAAAGCAGT GTGAAGTCCT TGACCTCGCT CTGGACACTA TCAAGCAATA CTTTCATGCA 
GGAGGTAATG GGCTGAAGAA AACCTTCCTG GAGAAGAGCT CAGATCTGCA GTCTCTACGC 
TATGCCCTGT CTCTCTACAC ACAGACCACA GATACACTCA TCAAAACCTT TGTACGCTCA 
CAGACTGCCC AGGGATCTGG TGTGGATGAT CCTGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTGTTTACAC ATCCTGGAAC TGGGGAGCAC AAGGTCACAG TGAAAGTGGT AGCTGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCCTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGAAAAGAA GAGGAAGTTC ACAACCAAGT CAAAAAGCAA CAACTGGGCT 
CCCAAGTACA ATGAGACATT TCACTTCCTC CTGGGAAATG AAGAAGGGCC AGAAGCCTAT 
GAATTGCAAA TATGTGTGAA GGATTACTGC TTTGCCCGTG AGGATCGTGT TTTGGGGCTG 
GCTGTGATGC CTCTGAGGGA TGTAGCAGCC AAGGGCAGCT GTGCTTGCTG GTGTCCCTTG 
GGCCGGAAGA TCCACATGGA TGAGACAGGC ATGACTATTC TCCGGATTCT ATCTCAAAGG 
AGCAATGACG AAGTGGCTCG AGAGTTTGTG AAGCTCAAAT CGGAGTCTCG TTCCATGGAA 
GAGGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281257.1
CDS	334..5145
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCCHWTYLGWGEQQTFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425549.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425549.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCAGCA GACTTTTGAA GATCCTGATA GTGCTGTTGA TGACCGGGAT 
AGTGACTATC GCAGTGAGAC CAGCAATAGC ATCCCACCTC CTTATCATAC AACTTCCCAG 
CCCAACGCTT CTGTGCACCA GTTTCCTGTG CCCATGCGAT TGCCACAGCA GCTGCTACTT 
CAGGGCAATT CCCGGGATTC TTGCAATGAC TCCATGCAAA GTTACGATCT TGATTATCCA 
GAGCGGCGGG CCCTTAGCCC CACCAGCAGT AGTAGGTATG GCTCATCCTG TAATGTGAGT 
CAAGGAAGCT CTCAGTTGAG TGAACTAGAC CAGTGTCACG AACAAGATGA TGACCGTCGG 
GAGAGGGATT CAATTCATTC ATGCCACAGC TCTGGCAGCT TCTCCAGAGA CGGCCAGGTG 
GGCTTTGGAG AACAAGAGAA AGCCTTGGAG GTAACAGGTG AAGAAGAGAA GGGGAGAGTG 
TGTGAACCCA AGGACAAGAA AAAAGATACC ACAACCCATC TTCCCCCAGA TCTGGTGCTA 
CCCAAAGACC ACGTCCTAGG CCCCCAGGAG AGTTTTCCTG AGGAGAATGG TTCTTCACCA 
TTTACCCAAG CAAGAGCTCA CTGGATCCGA GCAGTTACCA AGGTTCGACT CCAGCTGCAG 
GAGATTCCGG ATGATGGTCA CCCTTCTTTG CCCCAGTGGC TTCCGGAAGG GCCTGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGCAGAA AGAAACCATT GCCACTTGTC 
AGTGATCTGT CATTGGTCCA GTCCCGGAAA GCAGGAATTA CTTCTGCAAT GGCTACACGT 
ACCTCTCTGA AGGATGAAGA GCTGAAATCC CATGTGTATA AGAAAACCCT GCAGGCCTTA 
ATCTACCCAA TCTCGTGCAC CACACCCCAC AACTTTGAAG TCTGGACAGC CACTACCCCA 
ACCTACTGCT ATGAGTGTGA AGGTCTGCTC TGGGGCATTG CCCGGCAGGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAAGACC TGCTCAACGC CGACTGCCTG 
CAGCGGGCTG CAGAAAAGAG TTCTAAACAT GGAGCTGAGG ACCGGACCCA GAACATAATC 
ATGGCGATGA AGGACCGCAT GAAGATCCGA GAGCGGAATA AGCCAGAGAT CTTTGAGGTT 
ATCCGAGATG TCTTTACCGT GAGCAAAGTT GCCCATGTGC AACAGATGAA AACAGTGAAG 
CAGAGTGTAC TGGATGGCAC CTCCAAATGG TCAGCCAAAA TTACCATCAC TGTGGTGTGT 
GCCCAGGGCC TACAAGCCAA GGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTGGGCAAAA CCAAGAAGCG TACCAAGACT ATTTTTGGGA ACCTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGACCGCA TTAAGGTACG TGTATGGGAT 
GAGGATGATG ACATCAAGTC AAGAGTAAAG CAACGGCTAA AGCGAGAGTC CGATGATTTC 
CTTGGCCAAA CCATTATTGA GGTTCGGACA CTGAGTGGCG AGATGGACGT CTGGTACAAC 
TTGGAGAAGA GGACAGATAA ATCAGCTGTC TCAGGGGCTA TCCGACTGCA AATCAGTGTG 
GAGATCAAGG GAGAGGAGAA AGTAGCGCCA TATCATGTGC AGTATACGTG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGTAGTGGAG GAGTCCTGAT CCCTGAGGCT 
CGCGGAGACG ATGCATGGAA GGTGTACTTT GATGAGACTG CCCAAGAAAT TGTGGATGAA 
TTTGCCATGC GCTATGGCAT CGAGTCCATA TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTGCCT GCGGTGATGA GCACCTTATT GGCCAACATC 
AATGCCTACT ATGCCCATAC AACTGCCTCT ACCAACGTCT CTGCATCTGA TCGCTTTGCA 
GCCTCCAACT TTGGGAAAGA GAGATTTGTA AAACTACTGG ACCAGCTGCA CAACTCACTG 
AGGATTGACC TCTCTACCTA CAGGAATAAT TTCCCTGCTG GAAGCCCTGA GCGGCTTCAG 
GACTTAAAGT CAACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGAAT GAAGGTACAA 
GAACTGCAGA GCCCTCCAAG AGCCAGCCAA GTGGTAAAGG ATTGTGTGAA GGCCTGTTTG 
AACTCCACAT ATGAGTATAT CTTCAACAAC TGCCATGACT TGTATAGTCG CCAGTACCAG 
CTGAAACAGG AGCTACCTCC AGAGGAACAA GGACCCAGCA TTCGGAACCT GGATTTTTGG 
CCCAAACTCA TCACGCTCAT TGTGTCAATC ATAGAGGAAG ATAAGAATTC CTACACACTC 
GTTCTGAACC AGTTTCCTCA GGAATTGAAT GTGGGAAAAG TCAGCGCAGA AGTGATGTGG 
CAGCTCTTTG CCCAAGACAT GAAATATGCA CTGGAGGAGC ATGAGAAGGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATACGTG 
CGAGATCTGC CTGCCCTGCA GGGGCAGGTG CCTGAGTACC CAGTGTGGTT TGAGCAGTTT 
GTTCTGCAAT GGCTGGATGA AAATGAGGAT GTGTCCCTGG AATTCCTGCG TGGGGCTCTG 
GAACGAGATA AGAAGGATGG GTTCCAGCAG ACATCAGAGC ATGCACTCTT TTCCTGCTCT 
GTGGTGGATG TCTTCACACA GCTCAACCAG AGCTTTGAGA TCATCCGGAA GCTGGAGTGC 
CCAGACCCCA ACATCCTTGC CCACTACATG AGGAGATTTG CTAAGACCAT CGGGAAGGTG 
TTGATGCAGT ATGCAAACAT CTTATCAAAG AGCTTCCCAG ATTATTGCAC AAAGGAGAAA 
ATGCCCTGCA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAGGA GTTGGACCCT GAAGCTGCAG ACAGTTTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTC AGCATGGTAT TTGGAAACAG TTTTCAGGTG 
CGGATTGATG AGTGTGTTCG ACAAATGGCT GACATCCTGG GCCAGGTGCG AGGCCCAGGG 
AATGCATCCC CCAACGCCAG GGCCTCTGTG GCTCAGGATG CAGATAGTGT GCTCCGGCCT 
CTCATGGACT TCCTGGATGG CAACCTCACA CTCTTTGCCA CTGTGTGTGA GAAGACAGTC 
CTGAAGCGTG TACTGAAGGA GCTCTGGAGG GTGGTCATGA ACACAATGGA AAGAATGATT 
GTTCTGCCCC CACTCACTGA CCAGACGGGC ACCCAGCTGA TCTTTACTGC TGCCAAGGAG 
CTGAGCCATC TTTCTAAGCT CAAGGACCAC ATGGTGCGAG AGGAAACACG GAGTCTCACT 
CCAAAGCAGT GTGAAGTCCT TGACCTCGCT CTGGACACTA TCAAGCAATA CTTTCATGCA 
GGAGGTAATG GGCTGAAGAA AACCTTCCTG GAGAAGAGCT CAGATCTGCA GTCTCTACGC 
TATGCCCTGT CTCTCTACAC ACAGACCACA GATACACTCA TCAAAACCTT TGTACGCTCA 
CAGACTGCCC AGGGATCTGG TGTGGATGAT CCTGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTGTTTACAC ATCCTGGAAC TGGGGAGCAC AAGGTCACAG TGAAAGTGGT AGCTGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCCTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGAAAAGAA GAGGAAGTTC ACAACCAAGT CAAAAAGCAA CAACTGGGCT 
CCCAAGTACA ATGAGACATT TCACTTCCTC CTGGGAAATG AAGAAGGGCC AGAAGCCTAT 
GAATTGCAAA TATGTGTGAA GGATTACTGC TTTGCCCGTG AGGATCGTGT TTTGGGGCTG 
GCTGTGATGC CTCTGAGGGA TGTAGCAGCC AAGGGCAGCT GTGCTTGCTG GTGTCCCTTG 
GGCCGGAAGA TCCACATGGA TGAGACAGGC ATGACTATTC TCCGGATTCT ATCTCAAAGG 
AGCAATGACG AAGTGGCTCG AGAGTTTGTG AAGCTCAAAT CGGAGTCTCG TTCCATGGAA 
GAGGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_022425550.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281258.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X9, mRNA.

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CloneID	OCe14579
Clone ID Related Accession (Same CDS sequence)	XM_022425550.1
Accession Version	XM_022425550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT 
GATAGTGCTG TTGATGACCG GGATAGTGAC TATCGCAGTG AGACCAGCAA TAGCATCCCA 
CCTCCTTATC ATACAACTTC CCAGCCCAAC GCTTCTGTGC ACCAGTTTCC TGTGCCCATG 
CGATTGCCAC AGCAGCTGCT ACTTCAGGGC AATTCCCGGG ATTCTTGCAA TGACTCCATG 
CAAAGTTACG ATCTTGATTA TCCAGAGCGG CGGGCCCTTA GCCCCACCAG CAGTAGTAGG 
TATGGCTCAT CCTGTAATGT GAGTCAAGGA AGCTCTCAGT TGAGTGAACT AGACCAGTGT 
CACGAACAAG ATGATGACCG TCGGGAGAGG GATTCAATTC ATTCATGCCA CAGCTCTGGC 
AGCTTCTCCA GAGACGGCCA GGTGGGCTTT GGAGAACAAG AGAAAGCCTT GGAGGTAACA 
GGTGAAGAAG AGAAGGGGAG AGTGTGTGAA CCCAAGGACA AGAAAAAAGA TACCACAACC 
CATCTTCCCC CAGATCTGGT GCTACCCAAA GACCACGTCC TAGGCCCCCA GGAGAGTTTT 
CCTGAGGAGA ATGGTTCTTC ACCATTTACC CAAGCAAGAG CTCACTGGAT CCGAGCAGTT 
ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCGGATGATG GTCACCCTTC TTTGCCCCAG 
TGGCTTCCGG AAGGGCCTGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGC 
AGAAAGAAAC CATTGCCACT TGTCAGTGAT CTGTCATTGG TCCAGTCCCG GAAAGCAGGA 
ATTACTTCTG CAATGGCTAC ACGTACCTCT CTGAAGGATG AAGAGCTGAA ATCCCATGTG 
TATAAGAAAA CCCTGCAGGC CTTAATCTAC CCAATCTCGT GCACCACACC CCACAACTTT 
GAAGTCTGGA CAGCCACTAC CCCAACCTAC TGCTATGAGT GTGAAGGTCT GCTCTGGGGC 
ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAA 
GACCTGCTCA ACGCCGACTG CCTGCAGCGG GCTGCAGAAA AGAGTTCTAA ACATGGAGCT 
GAGGACCGGA CCCAGAACAT AATCATGGCG ATGAAGGACC GCATGAAGAT CCGAGAGCGG 
AATAAGCCAG AGATCTTTGA GGTTATCCGA GATGTCTTTA CCGTGAGCAA AGTTGCCCAT 
GTGCAACAGA TGAAAACAGT GAAGCAGAGT GTACTGGATG GCACCTCCAA ATGGTCAGCC 
AAAATTACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACTATTTTT 
GGGAACCTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC 
CGCATTAAGG TACGTGTATG GGATGAGGAT GATGACATCA AGTCAAGAGT AAAGCAACGG 
CTAAAGCGAG AGTCCGATGA TTTCCTTGGC CAAACCATTA TTGAGGTTCG GACACTGAGT 
GGCGAGATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ATAAATCAGC TGTCTCAGGG 
GCTATCCGAC TGCAAATCAG TGTGGAGATC AAGGGAGAGG AGAAAGTAGC GCCATATCAT 
GTGCAGTATA CGTGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGTAGT 
GGAGGAGTCC TGATCCCTGA GGCTCGCGGA GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACTGCCCAAG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATCGAGTC CATATATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCTGCGGTG 
ATGAGCACCT TATTGGCCAA CATCAATGCC TACTATGCCC ATACAACTGC CTCTACCAAC 
GTCTCTGCAT CTGATCGCTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTAAAACTA 
CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT 
GCTGGAAGCC CTGAGCGGCT TCAGGACTTA AAGTCAACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GAATGAAGGT ACAAGAACTG CAGAGCCCTC CAAGAGCCAG CCAAGTGGTA 
AAGGATTGTG TGAAGGCCTG TTTGAACTCC ACATATGAGT ATATCTTCAA CAACTGCCAT 
GACTTGTATA GTCGCCAGTA CCAGCTGAAA CAGGAGCTAC CTCCAGAGGA ACAAGGACCC 
AGCATTCGGA ACCTGGATTT TTGGCCCAAA CTCATCACGC TCATTGTGTC AATCATAGAG 
GAAGATAAGA ATTCCTACAC ACTCGTTCTG AACCAGTTTC CTCAGGAATT GAATGTGGGA 
AAAGTCAGCG CAGAAGTGAT GTGGCAGCTC TTTGCCCAAG ACATGAAATA TGCACTGGAG 
GAGCATGAGA AGGACCGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG 
AAGTGGCTCC ACAATGAATA CGTGCGAGAT CTGCCTGCCC TGCAGGGGCA GGTGCCTGAG 
TACCCAGTGT GGTTTGAGCA GTTTGTTCTG CAATGGCTGG ATGAAAATGA GGATGTGTCC 
CTGGAATTCC TGCGTGGGGC TCTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA 
GAGCATGCAC TCTTTTCCTG CTCTGTGGTG GATGTCTTCA CACAGCTCAA CCAGAGCTTT 
GAGATCATCC GGAAGCTGGA GTGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA 
TTTGCTAAGA CCATCGGGAA GGTGTTGATG CAGTATGCAA ACATCTTATC AAAGAGCTTC 
CCAGATTATT GCACAAAGGA GAAAATGCCC TGCATCCTGA TGAACAACAT GCAGCAACTG 
AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC ATGGGAGGCA AGGAGTTGGA CCCTGAAGCT 
GCAGACAGTT TGAAGGAGCT GCAGGTGAAA CTGAATACAG TTCTGGATGA GCTCAGCATG 
GTATTTGGAA ACAGTTTTCA GGTGCGGATT GATGAGTGTG TTCGACAAAT GGCTGACATC 
CTGGGCCAGG TGCGAGGCCC AGGGAATGCA TCCCCCAACG CCAGGGCCTC TGTGGCTCAG 
GATGCAGATA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ATGGCAACCT CACACTCTTT 
GCCACTGTGT GTGAGAAGAC AGTCCTGAAG CGTGTACTGA AGGAGCTCTG GAGGGTGGTC 
ATGAACACAA TGGAAAGAAT GATTGTTCTG CCCCCACTCA CTGACCAGAC GGGCACCCAG 
CTGATCTTTA CTGCTGCCAA GGAGCTGAGC CATCTTTCTA AGCTCAAGGA CCACATGGTG 
CGAGAGGAAA CACGGAGTCT CACTCCAAAG CAGTGTGAAG TCCTTGACCT CGCTCTGGAC 
ACTATCAAGC AATACTTTCA TGCAGGAGGT AATGGGCTGA AGAAAACCTT CCTGGAGAAG 
AGCTCAGATC TGCAGTCTCT ACGCTATGCC CTGTCTCTCT ACACACAGAC CACAGATACA 
CTCATCAAAA CCTTTGTACG CTCACAGACT GCCCAGGGAT CTGGTGTGGA TGATCCTGTG 
GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC 
ACAGTGAAAG TGGTAGCTGC CAATGACCTC AAGTGGCAGA CAGCGGGTAT GTTCCGGCCC 
TTTGTGGAAG TGACCATGGT TGGCCCACAC CAAAGTGAAA AGAAGAGGAA GTTCACAACC 
AAGTCAAAAA GCAACAACTG GGCTCCCAAG TACAATGAGA CATTTCACTT CCTCCTGGGA 
AATGAAGAAG GGCCAGAAGC CTATGAATTG CAAATATGTG TGAAGGATTA CTGCTTTGCC 
CGTGAGGATC GTGTTTTGGG GCTGGCTGTG ATGCCTCTGA GGGATGTAGC AGCCAAGGGC 
AGCTGTGCTT GCTGGTGTCC CTTGGGCCGG AAGATCCACA TGGATGAGAC AGGCATGACT 
ATTCTCCGGA TTCTATCTCA AAGGAGCAAT GACGAAGTGG CTCGAGAGTT TGTGAAGCTC 
AAATCGGAGT CTCGTTCCAT GGAAGAGGGG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281258.1
CDS	334..5109
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425550.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425550.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT 
GATAGTGCTG TTGATGACCG GGATAGTGAC TATCGCAGTG AGACCAGCAA TAGCATCCCA 
CCTCCTTATC ATACAACTTC CCAGCCCAAC GCTTCTGTGC ACCAGTTTCC TGTGCCCATG 
CGATTGCCAC AGCAGCTGCT ACTTCAGGGC AATTCCCGGG ATTCTTGCAA TGACTCCATG 
CAAAGTTACG ATCTTGATTA TCCAGAGCGG CGGGCCCTTA GCCCCACCAG CAGTAGTAGG 
TATGGCTCAT CCTGTAATGT GAGTCAAGGA AGCTCTCAGT TGAGTGAACT AGACCAGTGT 
CACGAACAAG ATGATGACCG TCGGGAGAGG GATTCAATTC ATTCATGCCA CAGCTCTGGC 
AGCTTCTCCA GAGACGGCCA GGTGGGCTTT GGAGAACAAG AGAAAGCCTT GGAGGTAACA 
GGTGAAGAAG AGAAGGGGAG AGTGTGTGAA CCCAAGGACA AGAAAAAAGA TACCACAACC 
CATCTTCCCC CAGATCTGGT GCTACCCAAA GACCACGTCC TAGGCCCCCA GGAGAGTTTT 
CCTGAGGAGA ATGGTTCTTC ACCATTTACC CAAGCAAGAG CTCACTGGAT CCGAGCAGTT 
ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCGGATGATG GTCACCCTTC TTTGCCCCAG 
TGGCTTCCGG AAGGGCCTGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGC 
AGAAAGAAAC CATTGCCACT TGTCAGTGAT CTGTCATTGG TCCAGTCCCG GAAAGCAGGA 
ATTACTTCTG CAATGGCTAC ACGTACCTCT CTGAAGGATG AAGAGCTGAA ATCCCATGTG 
TATAAGAAAA CCCTGCAGGC CTTAATCTAC CCAATCTCGT GCACCACACC CCACAACTTT 
GAAGTCTGGA CAGCCACTAC CCCAACCTAC TGCTATGAGT GTGAAGGTCT GCTCTGGGGC 
ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAA 
GACCTGCTCA ACGCCGACTG CCTGCAGCGG GCTGCAGAAA AGAGTTCTAA ACATGGAGCT 
GAGGACCGGA CCCAGAACAT AATCATGGCG ATGAAGGACC GCATGAAGAT CCGAGAGCGG 
AATAAGCCAG AGATCTTTGA GGTTATCCGA GATGTCTTTA CCGTGAGCAA AGTTGCCCAT 
GTGCAACAGA TGAAAACAGT GAAGCAGAGT GTACTGGATG GCACCTCCAA ATGGTCAGCC 
AAAATTACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACTATTTTT 
GGGAACCTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC 
CGCATTAAGG TACGTGTATG GGATGAGGAT GATGACATCA AGTCAAGAGT AAAGCAACGG 
CTAAAGCGAG AGTCCGATGA TTTCCTTGGC CAAACCATTA TTGAGGTTCG GACACTGAGT 
GGCGAGATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ATAAATCAGC TGTCTCAGGG 
GCTATCCGAC TGCAAATCAG TGTGGAGATC AAGGGAGAGG AGAAAGTAGC GCCATATCAT 
GTGCAGTATA CGTGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGTAGT 
GGAGGAGTCC TGATCCCTGA GGCTCGCGGA GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACTGCCCAAG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATCGAGTC CATATATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCTGCGGTG 
ATGAGCACCT TATTGGCCAA CATCAATGCC TACTATGCCC ATACAACTGC CTCTACCAAC 
GTCTCTGCAT CTGATCGCTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTAAAACTA 
CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT 
GCTGGAAGCC CTGAGCGGCT TCAGGACTTA AAGTCAACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GAATGAAGGT ACAAGAACTG CAGAGCCCTC CAAGAGCCAG CCAAGTGGTA 
AAGGATTGTG TGAAGGCCTG TTTGAACTCC ACATATGAGT ATATCTTCAA CAACTGCCAT 
GACTTGTATA GTCGCCAGTA CCAGCTGAAA CAGGAGCTAC CTCCAGAGGA ACAAGGACCC 
AGCATTCGGA ACCTGGATTT TTGGCCCAAA CTCATCACGC TCATTGTGTC AATCATAGAG 
GAAGATAAGA ATTCCTACAC ACTCGTTCTG AACCAGTTTC CTCAGGAATT GAATGTGGGA 
AAAGTCAGCG CAGAAGTGAT GTGGCAGCTC TTTGCCCAAG ACATGAAATA TGCACTGGAG 
GAGCATGAGA AGGACCGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG 
AAGTGGCTCC ACAATGAATA CGTGCGAGAT CTGCCTGCCC TGCAGGGGCA GGTGCCTGAG 
TACCCAGTGT GGTTTGAGCA GTTTGTTCTG CAATGGCTGG ATGAAAATGA GGATGTGTCC 
CTGGAATTCC TGCGTGGGGC TCTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA 
GAGCATGCAC TCTTTTCCTG CTCTGTGGTG GATGTCTTCA CACAGCTCAA CCAGAGCTTT 
GAGATCATCC GGAAGCTGGA GTGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA 
TTTGCTAAGA CCATCGGGAA GGTGTTGATG CAGTATGCAA ACATCTTATC AAAGAGCTTC 
CCAGATTATT GCACAAAGGA GAAAATGCCC TGCATCCTGA TGAACAACAT GCAGCAACTG 
AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC ATGGGAGGCA AGGAGTTGGA CCCTGAAGCT 
GCAGACAGTT TGAAGGAGCT GCAGGTGAAA CTGAATACAG TTCTGGATGA GCTCAGCATG 
GTATTTGGAA ACAGTTTTCA GGTGCGGATT GATGAGTGTG TTCGACAAAT GGCTGACATC 
CTGGGCCAGG TGCGAGGCCC AGGGAATGCA TCCCCCAACG CCAGGGCCTC TGTGGCTCAG 
GATGCAGATA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ATGGCAACCT CACACTCTTT 
GCCACTGTGT GTGAGAAGAC AGTCCTGAAG CGTGTACTGA AGGAGCTCTG GAGGGTGGTC 
ATGAACACAA TGGAAAGAAT GATTGTTCTG CCCCCACTCA CTGACCAGAC GGGCACCCAG 
CTGATCTTTA CTGCTGCCAA GGAGCTGAGC CATCTTTCTA AGCTCAAGGA CCACATGGTG 
CGAGAGGAAA CACGGAGTCT CACTCCAAAG CAGTGTGAAG TCCTTGACCT CGCTCTGGAC 
ACTATCAAGC AATACTTTCA TGCAGGAGGT AATGGGCTGA AGAAAACCTT CCTGGAGAAG 
AGCTCAGATC TGCAGTCTCT ACGCTATGCC CTGTCTCTCT ACACACAGAC CACAGATACA 
CTCATCAAAA CCTTTGTACG CTCACAGACT GCCCAGGGAT CTGGTGTGGA TGATCCTGTG 
GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC 
ACAGTGAAAG TGGTAGCTGC CAATGACCTC AAGTGGCAGA CAGCGGGTAT GTTCCGGCCC 
TTTGTGGAAG TGACCATGGT TGGCCCACAC CAAAGTGAAA AGAAGAGGAA GTTCACAACC 
AAGTCAAAAA GCAACAACTG GGCTCCCAAG TACAATGAGA CATTTCACTT CCTCCTGGGA 
AATGAAGAAG GGCCAGAAGC CTATGAATTG CAAATATGTG TGAAGGATTA CTGCTTTGCC 
CGTGAGGATC GTGTTTTGGG GCTGGCTGTG ATGCCTCTGA GGGATGTAGC AGCCAAGGGC 
AGCTGTGCTT GCTGGTGTCC CTTGGGCCGG AAGATCCACA TGGATGAGAC AGGCATGACT 
ATTCTCCGGA TTCTATCTCA AAGGAGCAAT GACGAAGTGG CTCGAGAGTT TGTGAAGCTC 
AAATCGGAGT CTCGTTCCAT GGAAGAGGGG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_022425551.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281259.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X10, mRNA.

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CloneID	OCe292142
Clone ID Related Accession (Same CDS sequence)	XM_022425551.1
Accession Version	XM_022425551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGCTTTTG AAGATCCTGA TAGTGCTGTT 
GATGACCGGG ATAGTGACTA TCGCAGTGAG ACCAGCAATA GCATCCCACC TCCTTATCAT 
ACAACTTCCC AGCCCAACGC TTCTGTGCAC CAGTTTCCTG TGCCCATGCG ATTGCCACAG 
CAGCTGCTAC TTCAGGGCAA TTCCCGGGAT TCTTGCAATG ACTCCATGCA AAGTTACGAT 
CTTGATTATC CAGAGCGGCG GGCCCTTAGC CCCACCAGCA GTAGTAGGTA TGGCTCATCC 
TGTAATGTGA GTCAAGGAAG CTCTCAGTTG AGTGAACTAG ACCAGTGTCA CGAACAAGAT 
GATGACCGTC GGGAGAGGGA TTCAATTCAT TCATGCCACA GCTCTGGCAG CTTCTCCAGA 
GACGGCCAGG TGGGCTTTGG AGAACAAGAG AAAGCCTTGG AGGTAACAGG TGAAGAAGAG 
AAGGGGAGAG TGTGTGAACC CAAGGACAAG AAAAAAGATA CCACAACCCA TCTTCCCCCA 
GATCTGGTGC TACCCAAAGA CCACGTCCTA GGCCCCCAGG AGAGTTTTCC TGAGGAGAAT 
GGTTCTTCAC CATTTACCCA AGCAAGAGCT CACTGGATCC GAGCAGTTAC CAAGGTTCGA 
CTCCAGCTGC AGGAGATTCC GGATGATGGT CACCCTTCTT TGCCCCAGTG GCTTCCGGAA 
GGGCCTGCTG GAGGGCTCTA TGGCATTGAC AGCATGCCAG ATCTACGCAG AAAGAAACCA 
TTGCCACTTG TCAGTGATCT GGCTATGTCA TTGGTCCAGT CCCGGAAAGC AGGAATTACT 
TCTGCAATGG CTACACGTAC CTCTCTGAAG GATGAAGAGC TGAAATCCCA TGTGTATAAG 
AAAACCCTGC AGGCCTTAAT CTACCCAATC TCGTGCACCA CACCCCACAA CTTTGAAGTC 
TGGACAGCCA CTACCCCAAC CTACTGCTAT GAGTGTGAAG GTCTGCTCTG GGGCATTGCC 
CGGCAGGGCA TGCGCTGCAG CGAGTGTGGA GTCAAGTGCC ATGAGAAGTG CCAAGACCTG 
CTCAACGCCG ACTGCCTGCA GCGGGCTGCA GAAAAGAGTT CTAAACATGG AGCTGAGGAC 
CGGACCCAGA ACATAATCAT GGCGATGAAG GACCGCATGA AGATCCGAGA GCGGAATAAG 
CCAGAGATCT TTGAGGTTAT CCGAGATGTC TTTACCGTGA GCAAAGTTGC CCATGTGCAA 
CAGATGAAAA CAGTGAAGCA GAGTGTACTG GATGGCACCT CCAAATGGTC AGCCAAAATT 
ACCATCACTG TGGTGTGTGC CCAGGGCCTA CAAGCCAAGG ACAAGACAGG ATCCAGTGAC 
CCTTATGTGA CTGTGCAAGT GGGCAAAACC AAGAAGCGTA CCAAGACTAT TTTTGGGAAC 
CTGAATCCTG TTTGGGAAGA GAAGTTCCAT TTTGAGTGCC ACAACTCTTC TGACCGCATT 
AAGGTACGTG TATGGGATGA GGATGATGAC ATCAAGTCAA GAGTAAAGCA ACGGCTAAAG 
CGAGAGTCCG ATGATTTCCT TGGCCAAACC ATTATTGAGG TTCGGACACT GAGTGGCGAG 
ATGGACGTCT GGTACAACTT GGAGAAGAGG ACAGATAAAT CAGCTGTCTC AGGGGCTATC 
CGACTGCAAA TCAGTGTGGA GATCAAGGGA GAGGAGAAAG TAGCGCCATA TCATGTGCAG 
TATACGTGTC TCCATGAGAA CCTTTTCCAT TACCTCACAG ACATTCAGGG TAGTGGAGGA 
GTCCTGATCC CTGAGGCTCG CGGAGACGAT GCATGGAAGG TGTACTTTGA TGAGACTGCC 
CAAGAAATTG TGGATGAATT TGCCATGCGC TATGGCATCG AGTCCATATA TCAGGCCATG 
ACGCACTTTG CATGTTTGTC ATCCAAGTAC ATGTGTCCTG GTGTGCCTGC GGTGATGAGC 
ACCTTATTGG CCAACATCAA TGCCTACTAT GCCCATACAA CTGCCTCTAC CAACGTCTCT 
GCATCTGATC GCTTTGCAGC CTCCAACTTT GGGAAAGAGA GATTTGTAAA ACTACTGGAC 
CAGCTGCACA ACTCACTGAG GATTGACCTC TCTACCTACA GGAATAATTT CCCTGCTGGA 
AGCCCTGAGC GGCTTCAGGA CTTAAAGTCA ACAGTGGATT TGCTGACCAG CATTACTTTC 
TTCAGAATGA AGGTACAAGA ACTGCAGAGC CCTCCAAGAG CCAGCCAAGT GGTAAAGGAT 
TGTGTGAAGG CCTGTTTGAA CTCCACATAT GAGTATATCT TCAACAACTG CCATGACTTG 
TATAGTCGCC AGTACCAGCT GAAACAGGAG CTACCTCCAG AGGAACAAGG ACCCAGCATT 
CGGAACCTGG ATTTTTGGCC CAAACTCATC ACGCTCATTG TGTCAATCAT AGAGGAAGAT 
AAGAATTCCT ACACACTCGT TCTGAACCAG TTTCCTCAGG AATTGAATGT GGGAAAAGTC 
AGCGCAGAAG TGATGTGGCA GCTCTTTGCC CAAGACATGA AATATGCACT GGAGGAGCAT 
GAGAAGGACC GGCTGTGTAA GAGTGCTGAC TACATGAACC TGCACTTCAA GGTGAAGTGG 
CTCCACAATG AATACGTGCG AGATCTGCCT GCCCTGCAGG GGCAGGTGCC TGAGTACCCA 
GTGTGGTTTG AGCAGTTTGT TCTGCAATGG CTGGATGAAA ATGAGGATGT GTCCCTGGAA 
TTCCTGCGTG GGGCTCTGGA ACGAGATAAG AAGGATGGGT TCCAGCAGAC ATCAGAGCAT 
GCACTCTTTT CCTGCTCTGT GGTGGATGTC TTCACACAGC TCAACCAGAG CTTTGAGATC 
ATCCGGAAGC TGGAGTGCCC AGACCCCAAC ATCCTTGCCC ACTACATGAG GAGATTTGCT 
AAGACCATCG GGAAGGTGTT GATGCAGTAT GCAAACATCT TATCAAAGAG CTTCCCAGAT 
TATTGCACAA AGGAGAAAAT GCCCTGCATC CTGATGAACA ACATGCAGCA ACTGAGGGTC 
CAGCTGGAGA AAATGTTTGA GGCCATGGGA GGCAAGGAGT TGGACCCTGA AGCTGCAGAC 
AGTTTGAAGG AGCTGCAGGT GAAACTGAAT ACAGTTCTGG ATGAGCTCAG CATGGTATTT 
GGAAACAGTT TTCAGGTGCG GATTGATGAG TGTGTTCGAC AAATGGCTGA CATCCTGGGC 
CAGGTGCGAG GCCCAGGGAA TGCATCCCCC AACGCCAGGG CCTCTGTGGC TCAGGATGCA 
GATAGTGTGC TCCGGCCTCT CATGGACTTC CTGGATGGCA ACCTCACACT CTTTGCCACT 
GTGTGTGAGA AGACAGTCCT GAAGCGTGTA CTGAAGGAGC TCTGGAGGGT GGTCATGAAC 
ACAATGGAAA GAATGATTGT TCTGCCCCCA CTCACTGACC AGACGGGCAC CCAGCTGATC 
TTTACTGCTG CCAAGGAGCT GAGCCATCTT TCTAAGCTCA AGGACCACAT GGTGCGAGAG 
GAAACACGGA GTCTCACTCC AAAGCAGTGT GAAGTCCTTG ACCTCGCTCT GGACACTATC 
AAGCAATACT TTCATGCAGG AGGTAATGGG CTGAAGAAAA CCTTCCTGGA GAAGAGCTCA 
GATCTGCAGT CTCTACGCTA TGCCCTGTCT CTCTACACAC AGACCACAGA TACACTCATC 
AAAACCTTTG TACGCTCACA GACTGCCCAG GTGCATGATG GGAAAGGTAT TAGGTTTACT 
GCTAATGAGG ACATTCGGCC GGAAAAGGGA TCTGGTGTGG ATGATCCTGT GGGAGAAGTC 
TCTATTCAGG TGGACTTGTT TACACATCCT GGAACTGGGG AGCACAAGGT CACAGTGAAA 
GTGGTAGCTG CCAATGACCT CAAGTGGCAG ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA 
GTGACCATGG TTGGCCCACA CCAAAGTGAA AAGAAGAGGA AGTTCACAAC CAAGTCAAAA 
AGCAACAACT GGGCTCCCAA GTACAATGAG ACATTTCACT TCCTCCTGGG AAATGAAGAA 
GGGCCAGAAG CCTATGAATT GCAAATATGT GTGAAGGATT ACTGCTTTGC CCGTGAGGAT 
CGTGTTTTGG GGCTGGCTGT GATGCCTCTG AGGGATGTAG CAGCCAAGGG CAGCTGTGCT 
TGCTGGTGTC CCTTGGGCCG GAAGATCCAC ATGGATGAGA CAGGCATGAC TATTCTCCGG 
ATTCTATCTC AAAGGAGCAA TGACGAAGTG GCTCGAGAGT TTGTGAAGCT CAAATCGGAG 
TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281259.1
CDS	335..5041
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFAFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_022425551.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425551.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGCTTTTG AAGATCCTGA TAGTGCTGTT 
GATGACCGGG ATAGTGACTA TCGCAGTGAG ACCAGCAATA GCATCCCACC TCCTTATCAT 
ACAACTTCCC AGCCCAACGC TTCTGTGCAC CAGTTTCCTG TGCCCATGCG ATTGCCACAG 
CAGCTGCTAC TTCAGGGCAA TTCCCGGGAT TCTTGCAATG ACTCCATGCA AAGTTACGAT 
CTTGATTATC CAGAGCGGCG GGCCCTTAGC CCCACCAGCA GTAGTAGGTA TGGCTCATCC 
TGTAATGTGA GTCAAGGAAG CTCTCAGTTG AGTGAACTAG ACCAGTGTCA CGAACAAGAT 
GATGACCGTC GGGAGAGGGA TTCAATTCAT TCATGCCACA GCTCTGGCAG CTTCTCCAGA 
GACGGCCAGG TGGGCTTTGG AGAACAAGAG AAAGCCTTGG AGGTAACAGG TGAAGAAGAG 
AAGGGGAGAG TGTGTGAACC CAAGGACAAG AAAAAAGATA CCACAACCCA TCTTCCCCCA 
GATCTGGTGC TACCCAAAGA CCACGTCCTA GGCCCCCAGG AGAGTTTTCC TGAGGAGAAT 
GGTTCTTCAC CATTTACCCA AGCAAGAGCT CACTGGATCC GAGCAGTTAC CAAGGTTCGA 
CTCCAGCTGC AGGAGATTCC GGATGATGGT CACCCTTCTT TGCCCCAGTG GCTTCCGGAA 
GGGCCTGCTG GAGGGCTCTA TGGCATTGAC AGCATGCCAG ATCTACGCAG AAAGAAACCA 
TTGCCACTTG TCAGTGATCT GGCTATGTCA TTGGTCCAGT CCCGGAAAGC AGGAATTACT 
TCTGCAATGG CTACACGTAC CTCTCTGAAG GATGAAGAGC TGAAATCCCA TGTGTATAAG 
AAAACCCTGC AGGCCTTAAT CTACCCAATC TCGTGCACCA CACCCCACAA CTTTGAAGTC 
TGGACAGCCA CTACCCCAAC CTACTGCTAT GAGTGTGAAG GTCTGCTCTG GGGCATTGCC 
CGGCAGGGCA TGCGCTGCAG CGAGTGTGGA GTCAAGTGCC ATGAGAAGTG CCAAGACCTG 
CTCAACGCCG ACTGCCTGCA GCGGGCTGCA GAAAAGAGTT CTAAACATGG AGCTGAGGAC 
CGGACCCAGA ACATAATCAT GGCGATGAAG GACCGCATGA AGATCCGAGA GCGGAATAAG 
CCAGAGATCT TTGAGGTTAT CCGAGATGTC TTTACCGTGA GCAAAGTTGC CCATGTGCAA 
CAGATGAAAA CAGTGAAGCA GAGTGTACTG GATGGCACCT CCAAATGGTC AGCCAAAATT 
ACCATCACTG TGGTGTGTGC CCAGGGCCTA CAAGCCAAGG ACAAGACAGG ATCCAGTGAC 
CCTTATGTGA CTGTGCAAGT GGGCAAAACC AAGAAGCGTA CCAAGACTAT TTTTGGGAAC 
CTGAATCCTG TTTGGGAAGA GAAGTTCCAT TTTGAGTGCC ACAACTCTTC TGACCGCATT 
AAGGTACGTG TATGGGATGA GGATGATGAC ATCAAGTCAA GAGTAAAGCA ACGGCTAAAG 
CGAGAGTCCG ATGATTTCCT TGGCCAAACC ATTATTGAGG TTCGGACACT GAGTGGCGAG 
ATGGACGTCT GGTACAACTT GGAGAAGAGG ACAGATAAAT CAGCTGTCTC AGGGGCTATC 
CGACTGCAAA TCAGTGTGGA GATCAAGGGA GAGGAGAAAG TAGCGCCATA TCATGTGCAG 
TATACGTGTC TCCATGAGAA CCTTTTCCAT TACCTCACAG ACATTCAGGG TAGTGGAGGA 
GTCCTGATCC CTGAGGCTCG CGGAGACGAT GCATGGAAGG TGTACTTTGA TGAGACTGCC 
CAAGAAATTG TGGATGAATT TGCCATGCGC TATGGCATCG AGTCCATATA TCAGGCCATG 
ACGCACTTTG CATGTTTGTC ATCCAAGTAC ATGTGTCCTG GTGTGCCTGC GGTGATGAGC 
ACCTTATTGG CCAACATCAA TGCCTACTAT GCCCATACAA CTGCCTCTAC CAACGTCTCT 
GCATCTGATC GCTTTGCAGC CTCCAACTTT GGGAAAGAGA GATTTGTAAA ACTACTGGAC 
CAGCTGCACA ACTCACTGAG GATTGACCTC TCTACCTACA GGAATAATTT CCCTGCTGGA 
AGCCCTGAGC GGCTTCAGGA CTTAAAGTCA ACAGTGGATT TGCTGACCAG CATTACTTTC 
TTCAGAATGA AGGTACAAGA ACTGCAGAGC CCTCCAAGAG CCAGCCAAGT GGTAAAGGAT 
TGTGTGAAGG CCTGTTTGAA CTCCACATAT GAGTATATCT TCAACAACTG CCATGACTTG 
TATAGTCGCC AGTACCAGCT GAAACAGGAG CTACCTCCAG AGGAACAAGG ACCCAGCATT 
CGGAACCTGG ATTTTTGGCC CAAACTCATC ACGCTCATTG TGTCAATCAT AGAGGAAGAT 
AAGAATTCCT ACACACTCGT TCTGAACCAG TTTCCTCAGG AATTGAATGT GGGAAAAGTC 
AGCGCAGAAG TGATGTGGCA GCTCTTTGCC CAAGACATGA AATATGCACT GGAGGAGCAT 
GAGAAGGACC GGCTGTGTAA GAGTGCTGAC TACATGAACC TGCACTTCAA GGTGAAGTGG 
CTCCACAATG AATACGTGCG AGATCTGCCT GCCCTGCAGG GGCAGGTGCC TGAGTACCCA 
GTGTGGTTTG AGCAGTTTGT TCTGCAATGG CTGGATGAAA ATGAGGATGT GTCCCTGGAA 
TTCCTGCGTG GGGCTCTGGA ACGAGATAAG AAGGATGGGT TCCAGCAGAC ATCAGAGCAT 
GCACTCTTTT CCTGCTCTGT GGTGGATGTC TTCACACAGC TCAACCAGAG CTTTGAGATC 
ATCCGGAAGC TGGAGTGCCC AGACCCCAAC ATCCTTGCCC ACTACATGAG GAGATTTGCT 
AAGACCATCG GGAAGGTGTT GATGCAGTAT GCAAACATCT TATCAAAGAG CTTCCCAGAT 
TATTGCACAA AGGAGAAAAT GCCCTGCATC CTGATGAACA ACATGCAGCA ACTGAGGGTC 
CAGCTGGAGA AAATGTTTGA GGCCATGGGA GGCAAGGAGT TGGACCCTGA AGCTGCAGAC 
AGTTTGAAGG AGCTGCAGGT GAAACTGAAT ACAGTTCTGG ATGAGCTCAG CATGGTATTT 
GGAAACAGTT TTCAGGTGCG GATTGATGAG TGTGTTCGAC AAATGGCTGA CATCCTGGGC 
CAGGTGCGAG GCCCAGGGAA TGCATCCCCC AACGCCAGGG CCTCTGTGGC TCAGGATGCA 
GATAGTGTGC TCCGGCCTCT CATGGACTTC CTGGATGGCA ACCTCACACT CTTTGCCACT 
GTGTGTGAGA AGACAGTCCT GAAGCGTGTA CTGAAGGAGC TCTGGAGGGT GGTCATGAAC 
ACAATGGAAA GAATGATTGT TCTGCCCCCA CTCACTGACC AGACGGGCAC CCAGCTGATC 
TTTACTGCTG CCAAGGAGCT GAGCCATCTT TCTAAGCTCA AGGACCACAT GGTGCGAGAG 
GAAACACGGA GTCTCACTCC AAAGCAGTGT GAAGTCCTTG ACCTCGCTCT GGACACTATC 
AAGCAATACT TTCATGCAGG AGGTAATGGG CTGAAGAAAA CCTTCCTGGA GAAGAGCTCA 
GATCTGCAGT CTCTACGCTA TGCCCTGTCT CTCTACACAC AGACCACAGA TACACTCATC 
AAAACCTTTG TACGCTCACA GACTGCCCAG GTGCATGATG GGAAAGGTAT TAGGTTTACT 
GCTAATGAGG ACATTCGGCC GGAAAAGGGA TCTGGTGTGG ATGATCCTGT GGGAGAAGTC 
TCTATTCAGG TGGACTTGTT TACACATCCT GGAACTGGGG AGCACAAGGT CACAGTGAAA 
GTGGTAGCTG CCAATGACCT CAAGTGGCAG ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA 
GTGACCATGG TTGGCCCACA CCAAAGTGAA AAGAAGAGGA AGTTCACAAC CAAGTCAAAA 
AGCAACAACT GGGCTCCCAA GTACAATGAG ACATTTCACT TCCTCCTGGG AAATGAAGAA 
GGGCCAGAAG CCTATGAATT GCAAATATGT GTGAAGGATT ACTGCTTTGC CCGTGAGGAT 
CGTGTTTTGG GGCTGGCTGT GATGCCTCTG AGGGATGTAG CAGCCAAGGG CAGCTGTGCT 
TGCTGGTGTC CCTTGGGCCG GAAGATCCAC ATGGATGAGA CAGGCATGAC TATTCTCCGG 
ATTCTATCTC AAAGGAGCAA TGACGAAGTG GCTCGAGAGT TTGTGAAGCT CAAATCGGAG 
TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117829.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973304.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X5, mRNA.

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CloneID	OCe39186
Clone ID Related Accession (Same CDS sequence)	XM_014117829.1
Accession Version	XM_014117829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTGT CACTGGACCT ATTTGGGCTG GGGAGAGCAG 
CAGACTTTTG AAGATCCTGA TAGTGCTGTT GATGACCGGG ATAGTGACTA TCGCAGTGAG 
ACCAGCAATA GCATCCCACC TCCTTATCAT ACAACTTCCC AGCCCAACGC TTCTGTGCAC 
CAGTTTCCTG TGCCCATGCG ATTGCCACAG CAGCTGCTAC TTCAGGGCAA TTCCCGGGAT 
TCTTGCAATG ACTCCATGCA AAGTTACGAT CTTGATTATC CAGAGCGGCG GGCCCTTAGC 
CCCACCAGCA GTAGTAGGTA TGGCTCATCC TGTAATGTGA GTCAAGGAAG CTCTCAGTTG 
AGTGAACTAG ACCAGTGTCA CGAACAAGAT GATGACCGTC GGGAGAGGGA TTCAATTCAT 
TCATGCCACA GCTCTGGCAG CTTCTCCAGA GACGGCCAGG TGGGCTTTGG AGAACAAGAG 
AAAGCCTTGG AGGTAACAGG TGAAGAAGAG AAGGGGAGAG TGTGTGAACC CAAGGACAAG 
AAAAAAGATA CCACAACCCA TCTTCCCCCA GATCTGGTGC TACCCAAAGA CCACGTCCTA 
GGCCCCCAGG AGAGTTTTCC TGAGGAGAAT GGTTCTTCAC CATTTACCCA AGCAAGAGCT 
CACTGGATCC GAGCAGTTAC CAAGGTTCGA CTCCAGCTGC AGGAGATTCC GGATGATGGT 
CACCCTTCTT TGCCCCAGTG GCTTCCGGAA GGGCCTGCTG GAGGGCTCTA TGGCATTGAC 
AGCATGCCAG ATCTACGCAG AAAGAAACCA TTGCCACTTG TCAGTGATCT GGCTATGTCA 
TTGGTCCAGT CCCGGAAAGC AGGAATTACT TCTGCAATGG CTACACGTAC CTCTCTGAAG 
GATGAAGAGC TGAAATCCCA TGTGTATAAG AAAACCCTGC AGGCCTTAAT CTACCCAATC 
TCGTGCACCA CACCCCACAA CTTTGAAGTC TGGACAGCCA CTACCCCAAC CTACTGCTAT 
GAGTGTGAAG GTCTGCTCTG GGGCATTGCC CGGCAGGGCA TGCGCTGCAG CGAGTGTGGA 
GTCAAGTGCC ATGAGAAGTG CCAAGACCTG CTCAACGCCG ACTGCCTGCA GCGGGCTGCA 
GAAAAGAGTT CTAAACATGG AGCTGAGGAC CGGACCCAGA ACATAATCAT GGCGATGAAG 
GACCGCATGA AGATCCGAGA GCGGAATAAG CCAGAGATCT TTGAGGTTAT CCGAGATGTC 
TTTACCGTGA GCAAAGTTGC CCATGTGCAA CAGATGAAAA CAGTGAAGCA GAGTGTACTG 
GATGGCACCT CCAAATGGTC AGCCAAAATT ACCATCACTG TGGTGTGTGC CCAGGGCCTA 
CAAGCCAAGG ACAAGACAGG ATCCAGTGAC CCTTATGTGA CTGTGCAAGT GGGCAAAACC 
AAGAAGCGTA CCAAGACTAT TTTTGGGAAC CTGAATCCTG TTTGGGAAGA GAAGTTCCAT 
TTTGAGTGCC ACAACTCTTC TGACCGCATT AAGGTACGTG TATGGGATGA GGATGATGAC 
ATCAAGTCAA GAGTAAAGCA ACGGCTAAAG CGAGAGTCCG ATGATTTCCT TGGCCAAACC 
ATTATTGAGG TTCGGACACT GAGTGGCGAG ATGGACGTCT GGTACAACTT GGAGAAGAGG 
ACAGATAAAT CAGCTGTCTC AGGGGCTATC CGACTGCAAA TCAGTGTGGA GATCAAGGGA 
GAGGAGAAAG TAGCGCCATA TCATGTGCAG TATACGTGTC TCCATGAGAA CCTTTTCCAT 
TACCTCACAG ACATTCAGGG TAGTGGAGGA GTCCTGATCC CTGAGGCTCG CGGAGACGAT 
GCATGGAAGG TGTACTTTGA TGAGACTGCC CAAGAAATTG TGGATGAATT TGCCATGCGC 
TATGGCATCG AGTCCATATA TCAGGCCATG ACGCACTTTG CATGTTTGTC ATCCAAGTAC 
ATGTGTCCTG GTGTGCCTGC GGTGATGAGC ACCTTATTGG CCAACATCAA TGCCTACTAT 
GCCCATACAA CTGCCTCTAC CAACGTCTCT GCATCTGATC GCTTTGCAGC CTCCAACTTT 
GGGAAAGAGA GATTTGTAAA ACTACTGGAC CAGCTGCACA ACTCACTGAG GATTGACCTC 
TCTACCTACA GGAATAATTT CCCTGCTGGA AGCCCTGAGC GGCTTCAGGA CTTAAAGTCA 
ACAGTGGATT TGCTGACCAG CATTACTTTC TTCAGAATGA AGGTACAAGA ACTGCAGAGC 
CCTCCAAGAG CCAGCCAAGT GGTAAAGGAT TGTGTGAAGG CCTGTTTGAA CTCCACATAT 
GAGTATATCT TCAACAACTG CCATGACTTG TATAGTCGCC AGTACCAGCT GAAACAGGAG 
CTACCTCCAG AGGAACAAGG ACCCAGCATT CGGAACCTGG ATTTTTGGCC CAAACTCATC 
ACGCTCATTG TGTCAATCAT AGAGGAAGAT AAGAATTCCT ACACACTCGT TCTGAACCAG 
TTTCCTCAGG AATTGAATGT GGGAAAAGTC AGCGCAGAAG TGATGTGGCA GCTCTTTGCC 
CAAGACATGA AATATGCACT GGAGGAGCAT GAGAAGGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATACGTGCG AGATCTGCCT 
GCCCTGCAGG GGCAGGTGCC TGAGTACCCA GTGTGGTTTG AGCAGTTTGT TCTGCAATGG 
CTGGATGAAA ATGAGGATGT GTCCCTGGAA TTCCTGCGTG GGGCTCTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCACTCTTTT CCTGCTCTGT GGTGGATGTC 
TTCACACAGC TCAACCAGAG CTTTGAGATC ATCCGGAAGC TGGAGTGCCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG GAGATTTGCT AAGACCATCG GGAAGGTGTT GATGCAGTAT 
GCAAACATCT TATCAAAGAG CTTCCCAGAT TATTGCACAA AGGAGAAAAT GCCCTGCATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAGGAGT TGGACCCTGA AGCTGCAGAC AGTTTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTCAG CATGGTATTT GGAAACAGTT TTCAGGTGCG GATTGATGAG 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAGGTGCGAG GCCCAGGGAA TGCATCCCCC 
AACGCCAGGG CCTCTGTGGC TCAGGATGCA GATAGTGTGC TCCGGCCTCT CATGGACTTC 
CTGGATGGCA ACCTCACACT CTTTGCCACT GTGTGTGAGA AGACAGTCCT GAAGCGTGTA 
CTGAAGGAGC TCTGGAGGGT GGTCATGAAC ACAATGGAAA GAATGATTGT TCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTTACTGCTG CCAAGGAGCT GAGCCATCTT 
TCTAAGCTCA AGGACCACAT GGTGCGAGAG GAAACACGGA GTCTCACTCC AAAGCAGTGT 
GAAGTCCTTG ACCTCGCTCT GGACACTATC AAGCAATACT TTCATGCAGG AGGTAATGGG 
CTGAAGAAAA CCTTCCTGGA GAAGAGCTCA GATCTGCAGT CTCTACGCTA TGCCCTGTCT 
CTCTACACAC AGACCACAGA TACACTCATC AAAACCTTTG TACGCTCACA GACTGCCCAG 
GTGCATGATG GGAAAGGTAT TAGGTTTACT GCTAATGAGG ACATTCGGCC GGAAAAGGGA 
TCTGGTGTGG ATGATCCTGT GGGAGAAGTC TCTATTCAGG TGGACTTGTT TACACATCCT 
GGAACTGGGG AGCACAAGGT CACAGTGAAA GTGGTAGCTG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAA 
AAGAAGAGGA AGTTCACAAC CAAGTCAAAA AGCAACAACT GGGCTCCCAA GTACAATGAG 
ACATTTCACT TCCTCCTGGG AAATGAAGAA GGGCCAGAAG CCTATGAATT GCAAATATGT 
GTGAAGGATT ACTGCTTTGC CCGTGAGGAT CGTGTTTTGG GGCTGGCTGT GATGCCTCTG 
AGGGATGTAG CAGCCAAGGG CAGCTGTGCT TGCTGGTGTC CCTTGGGCCG GAAGATCCAC 
ATGGATGAGA CAGGCATGAC TATTCTCCGG ATTCTATCTC AAAGGAGCAA TGACGAAGTG 
GCTCGAGAGT TTGTGAAGCT CAAATCGGAG TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973304.1
CDS	17..5053
Translation	MGEGCGVCKVVRGLAGVCEDATIWLLGSQVCEVEQMSAAYAVQENLSSGRWAMTARSLPLEVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCCHWTYLGWGEQQTFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_014117829.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117829.1

ORF Insert Sequence:

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTGT CACTGGACCT ATTTGGGCTG GGGAGAGCAG 
CAGACTTTTG AAGATCCTGA TAGTGCTGTT GATGACCGGG ATAGTGACTA TCGCAGTGAG 
ACCAGCAATA GCATCCCACC TCCTTATCAT ACAACTTCCC AGCCCAACGC TTCTGTGCAC 
CAGTTTCCTG TGCCCATGCG ATTGCCACAG CAGCTGCTAC TTCAGGGCAA TTCCCGGGAT 
TCTTGCAATG ACTCCATGCA AAGTTACGAT CTTGATTATC CAGAGCGGCG GGCCCTTAGC 
CCCACCAGCA GTAGTAGGTA TGGCTCATCC TGTAATGTGA GTCAAGGAAG CTCTCAGTTG 
AGTGAACTAG ACCAGTGTCA CGAACAAGAT GATGACCGTC GGGAGAGGGA TTCAATTCAT 
TCATGCCACA GCTCTGGCAG CTTCTCCAGA GACGGCCAGG TGGGCTTTGG AGAACAAGAG 
AAAGCCTTGG AGGTAACAGG TGAAGAAGAG AAGGGGAGAG TGTGTGAACC CAAGGACAAG 
AAAAAAGATA CCACAACCCA TCTTCCCCCA GATCTGGTGC TACCCAAAGA CCACGTCCTA 
GGCCCCCAGG AGAGTTTTCC TGAGGAGAAT GGTTCTTCAC CATTTACCCA AGCAAGAGCT 
CACTGGATCC GAGCAGTTAC CAAGGTTCGA CTCCAGCTGC AGGAGATTCC GGATGATGGT 
CACCCTTCTT TGCCCCAGTG GCTTCCGGAA GGGCCTGCTG GAGGGCTCTA TGGCATTGAC 
AGCATGCCAG ATCTACGCAG AAAGAAACCA TTGCCACTTG TCAGTGATCT GGCTATGTCA 
TTGGTCCAGT CCCGGAAAGC AGGAATTACT TCTGCAATGG CTACACGTAC CTCTCTGAAG 
GATGAAGAGC TGAAATCCCA TGTGTATAAG AAAACCCTGC AGGCCTTAAT CTACCCAATC 
TCGTGCACCA CACCCCACAA CTTTGAAGTC TGGACAGCCA CTACCCCAAC CTACTGCTAT 
GAGTGTGAAG GTCTGCTCTG GGGCATTGCC CGGCAGGGCA TGCGCTGCAG CGAGTGTGGA 
GTCAAGTGCC ATGAGAAGTG CCAAGACCTG CTCAACGCCG ACTGCCTGCA GCGGGCTGCA 
GAAAAGAGTT CTAAACATGG AGCTGAGGAC CGGACCCAGA ACATAATCAT GGCGATGAAG 
GACCGCATGA AGATCCGAGA GCGGAATAAG CCAGAGATCT TTGAGGTTAT CCGAGATGTC 
TTTACCGTGA GCAAAGTTGC CCATGTGCAA CAGATGAAAA CAGTGAAGCA GAGTGTACTG 
GATGGCACCT CCAAATGGTC AGCCAAAATT ACCATCACTG TGGTGTGTGC CCAGGGCCTA 
CAAGCCAAGG ACAAGACAGG ATCCAGTGAC CCTTATGTGA CTGTGCAAGT GGGCAAAACC 
AAGAAGCGTA CCAAGACTAT TTTTGGGAAC CTGAATCCTG TTTGGGAAGA GAAGTTCCAT 
TTTGAGTGCC ACAACTCTTC TGACCGCATT AAGGTACGTG TATGGGATGA GGATGATGAC 
ATCAAGTCAA GAGTAAAGCA ACGGCTAAAG CGAGAGTCCG ATGATTTCCT TGGCCAAACC 
ATTATTGAGG TTCGGACACT GAGTGGCGAG ATGGACGTCT GGTACAACTT GGAGAAGAGG 
ACAGATAAAT CAGCTGTCTC AGGGGCTATC CGACTGCAAA TCAGTGTGGA GATCAAGGGA 
GAGGAGAAAG TAGCGCCATA TCATGTGCAG TATACGTGTC TCCATGAGAA CCTTTTCCAT 
TACCTCACAG ACATTCAGGG TAGTGGAGGA GTCCTGATCC CTGAGGCTCG CGGAGACGAT 
GCATGGAAGG TGTACTTTGA TGAGACTGCC CAAGAAATTG TGGATGAATT TGCCATGCGC 
TATGGCATCG AGTCCATATA TCAGGCCATG ACGCACTTTG CATGTTTGTC ATCCAAGTAC 
ATGTGTCCTG GTGTGCCTGC GGTGATGAGC ACCTTATTGG CCAACATCAA TGCCTACTAT 
GCCCATACAA CTGCCTCTAC CAACGTCTCT GCATCTGATC GCTTTGCAGC CTCCAACTTT 
GGGAAAGAGA GATTTGTAAA ACTACTGGAC CAGCTGCACA ACTCACTGAG GATTGACCTC 
TCTACCTACA GGAATAATTT CCCTGCTGGA AGCCCTGAGC GGCTTCAGGA CTTAAAGTCA 
ACAGTGGATT TGCTGACCAG CATTACTTTC TTCAGAATGA AGGTACAAGA ACTGCAGAGC 
CCTCCAAGAG CCAGCCAAGT GGTAAAGGAT TGTGTGAAGG CCTGTTTGAA CTCCACATAT 
GAGTATATCT TCAACAACTG CCATGACTTG TATAGTCGCC AGTACCAGCT GAAACAGGAG 
CTACCTCCAG AGGAACAAGG ACCCAGCATT CGGAACCTGG ATTTTTGGCC CAAACTCATC 
ACGCTCATTG TGTCAATCAT AGAGGAAGAT AAGAATTCCT ACACACTCGT TCTGAACCAG 
TTTCCTCAGG AATTGAATGT GGGAAAAGTC AGCGCAGAAG TGATGTGGCA GCTCTTTGCC 
CAAGACATGA AATATGCACT GGAGGAGCAT GAGAAGGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATACGTGCG AGATCTGCCT 
GCCCTGCAGG GGCAGGTGCC TGAGTACCCA GTGTGGTTTG AGCAGTTTGT TCTGCAATGG 
CTGGATGAAA ATGAGGATGT GTCCCTGGAA TTCCTGCGTG GGGCTCTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCACTCTTTT CCTGCTCTGT GGTGGATGTC 
TTCACACAGC TCAACCAGAG CTTTGAGATC ATCCGGAAGC TGGAGTGCCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG GAGATTTGCT AAGACCATCG GGAAGGTGTT GATGCAGTAT 
GCAAACATCT TATCAAAGAG CTTCCCAGAT TATTGCACAA AGGAGAAAAT GCCCTGCATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAGGAGT TGGACCCTGA AGCTGCAGAC AGTTTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTCAG CATGGTATTT GGAAACAGTT TTCAGGTGCG GATTGATGAG 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAGGTGCGAG GCCCAGGGAA TGCATCCCCC 
AACGCCAGGG CCTCTGTGGC TCAGGATGCA GATAGTGTGC TCCGGCCTCT CATGGACTTC 
CTGGATGGCA ACCTCACACT CTTTGCCACT GTGTGTGAGA AGACAGTCCT GAAGCGTGTA 
CTGAAGGAGC TCTGGAGGGT GGTCATGAAC ACAATGGAAA GAATGATTGT TCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTTACTGCTG CCAAGGAGCT GAGCCATCTT 
TCTAAGCTCA AGGACCACAT GGTGCGAGAG GAAACACGGA GTCTCACTCC AAAGCAGTGT 
GAAGTCCTTG ACCTCGCTCT GGACACTATC AAGCAATACT TTCATGCAGG AGGTAATGGG 
CTGAAGAAAA CCTTCCTGGA GAAGAGCTCA GATCTGCAGT CTCTACGCTA TGCCCTGTCT 
CTCTACACAC AGACCACAGA TACACTCATC AAAACCTTTG TACGCTCACA GACTGCCCAG 
GTGCATGATG GGAAAGGTAT TAGGTTTACT GCTAATGAGG ACATTCGGCC GGAAAAGGGA 
TCTGGTGTGG ATGATCCTGT GGGAGAAGTC TCTATTCAGG TGGACTTGTT TACACATCCT 
GGAACTGGGG AGCACAAGGT CACAGTGAAA GTGGTAGCTG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAA 
AAGAAGAGGA AGTTCACAAC CAAGTCAAAA AGCAACAACT GGGCTCCCAA GTACAATGAG 
ACATTTCACT TCCTCCTGGG AAATGAAGAA GGGCCAGAAG CCTATGAATT GCAAATATGT 
GTGAAGGATT ACTGCTTTGC CCGTGAGGAT CGTGTTTTGG GGCTGGCTGT GATGCCTCTG 
AGGGATGTAG CAGCCAAGGG CAGCTGTGCT TGCTGGTGTC CCTTGGGCCG GAAGATCCAC 
ATGGATGAGA CAGGCATGAC TATTCTCCGG ATTCTATCTC AAAGGAGCAA TGACGAAGTG 
GCTCGAGAGT TTGTGAAGCT CAAATCGGAG TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117830.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973305.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe39187
Clone ID Related Accession (Same CDS sequence)	XM_014117830.1
Accession Version	XM_014117830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTCT TTTGAAGATC CTGATAGTGC TGTTGATGAC 
CGGGATAGTG ACTATCGCAG TGAGACCAGC AATAGCATCC CACCTCCTTA TCATACAACT 
TCCCAGCCCA ACGCTTCTGT GCACCAGTTT CCTGTGCCCA TGCGATTGCC ACAGCAGCTG 
CTACTTCAGG GCAATTCCCG GGATTCTTGC AATGACTCCA TGCAAAGTTA CGATCTTGAT 
TATCCAGAGC GGCGGGCCCT TAGCCCCACC AGCAGTAGTA GGTATGGCTC ATCCTGTAAT 
GTGAGTCAAG GAAGCTCTCA GTTGAGTGAA CTAGACCAGT GTCACGAACA AGATGATGAC 
CGTCGGGAGA GGGATTCAAT TCATTCATGC CACAGCTCTG GCAGCTTCTC CAGAGACGGC 
CAGGTGGGCT TTGGAGAACA AGAGAAAGCC TTGGAGGTAA CAGGTGAAGA AGAGAAGGGG 
AGAGTGTGTG AACCCAAGGA CAAGAAAAAA GATACCACAA CCCATCTTCC CCCAGATCTG 
GTGCTACCCA AAGACCACGT CCTAGGCCCC CAGGAGAGTT TTCCTGAGGA GAATGGTTCT 
TCACCATTTA CCCAAGCAAG AGCTCACTGG ATCCGAGCAG TTACCAAGGT TCGACTCCAG 
CTGCAGGAGA TTCCGGATGA TGGTCACCCT TCTTTGCCCC AGTGGCTTCC GGAAGGGCCT 
GCTGGAGGGC TCTATGGCAT TGACAGCATG CCAGATCTAC GCAGAAAGAA ACCATTGCCA 
CTTGTCAGTG ATCTGGCTAT GTCATTGGTC CAGTCCCGGA AAGCAGGAAT TACTTCTGCA 
ATGGCTACAC GTACCTCTCT GAAGGATGAA GAGCTGAAAT CCCATGTGTA TAAGAAAACC 
CTGCAGGCCT TAATCTACCC AATCTCGTGC ACCACACCCC ACAACTTTGA AGTCTGGACA 
GCCACTACCC CAACCTACTG CTATGAGTGT GAAGGTCTGC TCTGGGGCAT TGCCCGGCAG 
GGCATGCGCT GCAGCGAGTG TGGAGTCAAG TGCCATGAGA AGTGCCAAGA CCTGCTCAAC 
GCCGACTGCC TGCAGCGGGC TGCAGAAAAG AGTTCTAAAC ATGGAGCTGA GGACCGGACC 
CAGAACATAA TCATGGCGAT GAAGGACCGC ATGAAGATCC GAGAGCGGAA TAAGCCAGAG 
ATCTTTGAGG TTATCCGAGA TGTCTTTACC GTGAGCAAAG TTGCCCATGT GCAACAGATG 
AAAACAGTGA AGCAGAGTGT ACTGGATGGC ACCTCCAAAT GGTCAGCCAA AATTACCATC 
ACTGTGGTGT GTGCCCAGGG CCTACAAGCC AAGGACAAGA CAGGATCCAG TGACCCTTAT 
GTGACTGTGC AAGTGGGCAA AACCAAGAAG CGTACCAAGA CTATTTTTGG GAACCTGAAT 
CCTGTTTGGG AAGAGAAGTT CCATTTTGAG TGCCACAACT CTTCTGACCG CATTAAGGTA 
CGTGTATGGG ATGAGGATGA TGACATCAAG TCAAGAGTAA AGCAACGGCT AAAGCGAGAG 
TCCGATGATT TCCTTGGCCA AACCATTATT GAGGTTCGGA CACTGAGTGG CGAGATGGAC 
GTCTGGTACA ACTTGGAGAA GAGGACAGAT AAATCAGCTG TCTCAGGGGC TATCCGACTG 
CAAATCAGTG TGGAGATCAA GGGAGAGGAG AAAGTAGCGC CATATCATGT GCAGTATACG 
TGTCTCCATG AGAACCTTTT CCATTACCTC ACAGACATTC AGGGTAGTGG AGGAGTCCTG 
ATCCCTGAGG CTCGCGGAGA CGATGCATGG AAGGTGTACT TTGATGAGAC TGCCCAAGAA 
ATTGTGGATG AATTTGCCAT GCGCTATGGC ATCGAGTCCA TATATCAGGC CATGACGCAC 
TTTGCATGTT TGTCATCCAA GTACATGTGT CCTGGTGTGC CTGCGGTGAT GAGCACCTTA 
TTGGCCAACA TCAATGCCTA CTATGCCCAT ACAACTGCCT CTACCAACGT CTCTGCATCT 
GATCGCTTTG CAGCCTCCAA CTTTGGGAAA GAGAGATTTG TAAAACTACT GGACCAGCTG 
CACAACTCAC TGAGGATTGA CCTCTCTACC TACAGGAATA ATTTCCCTGC TGGAAGCCCT 
GAGCGGCTTC AGGACTTAAA GTCAACAGTG GATTTGCTGA CCAGCATTAC TTTCTTCAGA 
ATGAAGGTAC AAGAACTGCA GAGCCCTCCA AGAGCCAGCC AAGTGGTAAA GGATTGTGTG 
AAGGCCTGTT TGAACTCCAC ATATGAGTAT ATCTTCAACA ACTGCCATGA CTTGTATAGT 
CGCCAGTACC AGCTGAAACA GGAGCTACCT CCAGAGGAAC AAGGACCCAG CATTCGGAAC 
CTGGATTTTT GGCCCAAACT CATCACGCTC ATTGTGTCAA TCATAGAGGA AGATAAGAAT 
TCCTACACAC TCGTTCTGAA CCAGTTTCCT CAGGAATTGA ATGTGGGAAA AGTCAGCGCA 
GAAGTGATGT GGCAGCTCTT TGCCCAAGAC ATGAAATATG CACTGGAGGA GCATGAGAAG 
GACCGGCTGT GTAAGAGTGC TGACTACATG AACCTGCACT TCAAGGTGAA GTGGCTCCAC 
AATGAATACG TGCGAGATCT GCCTGCCCTG CAGGGGCAGG TGCCTGAGTA CCCAGTGTGG 
TTTGAGCAGT TTGTTCTGCA ATGGCTGGAT GAAAATGAGG ATGTGTCCCT GGAATTCCTG 
CGTGGGGCTC TGGAACGAGA TAAGAAGGAT GGGTTCCAGC AGACATCAGA GCATGCACTC 
TTTTCCTGCT CTGTGGTGGA TGTCTTCACA CAGCTCAACC AGAGCTTTGA GATCATCCGG 
AAGCTGGAGT GCCCAGACCC CAACATCCTT GCCCACTACA TGAGGAGATT TGCTAAGACC 
ATCGGGAAGG TGTTGATGCA GTATGCAAAC ATCTTATCAA AGAGCTTCCC AGATTATTGC 
ACAAAGGAGA AAATGCCCTG CATCCTGATG AACAACATGC AGCAACTGAG GGTCCAGCTG 
GAGAAAATGT TTGAGGCCAT GGGAGGCAAG GAGTTGGACC CTGAAGCTGC AGACAGTTTG 
AAGGAGCTGC AGGTGAAACT GAATACAGTT CTGGATGAGC TCAGCATGGT ATTTGGAAAC 
AGTTTTCAGG TGCGGATTGA TGAGTGTGTT CGACAAATGG CTGACATCCT GGGCCAGGTG 
CGAGGCCCAG GGAATGCATC CCCCAACGCC AGGGCCTCTG TGGCTCAGGA TGCAGATAGT 
GTGCTCCGGC CTCTCATGGA CTTCCTGGAT GGCAACCTCA CACTCTTTGC CACTGTGTGT 
GAGAAGACAG TCCTGAAGCG TGTACTGAAG GAGCTCTGGA GGGTGGTCAT GAACACAATG 
GAAAGAATGA TTGTTCTGCC CCCACTCACT GACCAGACGG GCACCCAGCT GATCTTTACT 
GCTGCCAAGG AGCTGAGCCA TCTTTCTAAG CTCAAGGACC ACATGGTGCG AGAGGAAACA 
CGGAGTCTCA CTCCAAAGCA GTGTGAAGTC CTTGACCTCG CTCTGGACAC TATCAAGCAA 
TACTTTCATG CAGGAGGTAA TGGGCTGAAG AAAACCTTCC TGGAGAAGAG CTCAGATCTG 
CAGTCTCTAC GCTATGCCCT GTCTCTCTAC ACACAGACCA CAGATACACT CATCAAAACC 
TTTGTACGCT CACAGACTGC CCAGGTGCAT GATGGGAAAG GTATTAGGTT TACTGCTAAT 
GAGGACATTC GGCCGGAAAA GGGATCTGGT GTGGATGATC CTGTGGGAGA AGTCTCTATT 
CAGGTGGACT TGTTTACACA TCCTGGAACT GGGGAGCACA AGGTCACAGT GAAAGTGGTA 
GCTGCCAATG ACCTCAAGTG GCAGACAGCG GGTATGTTCC GGCCCTTTGT GGAAGTGACC 
ATGGTTGGCC CACACCAAAG TGAAAAGAAG AGGAAGTTCA CAACCAAGTC AAAAAGCAAC 
AACTGGGCTC CCAAGTACAA TGAGACATTT CACTTCCTCC TGGGAAATGA AGAAGGGCCA 
GAAGCCTATG AATTGCAAAT ATGTGTGAAG GATTACTGCT TTGCCCGTGA GGATCGTGTT 
TTGGGGCTGG CTGTGATGCC TCTGAGGGAT GTAGCAGCCA AGGGCAGCTG TGCTTGCTGG 
TGTCCCTTGG GCCGGAAGAT CCACATGGAT GAGACAGGCA TGACTATTCT CCGGATTCTA 
TCTCAAAGGA GCAATGACGA AGTGGCTCGA GAGTTTGTGA AGCTCAAATC GGAGTCTCGT 
TCCATGGAAG AGGGGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973305.1
CDS	17..5017
Translation	MGEGCGVCKVVRGLAGVCEDATIWLLGSQVCEVEQMSAAYAVQENLSSGRWAMTARSLPLEVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_014117830.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117830.1

ORF Insert Sequence:

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTCT TTTGAAGATC CTGATAGTGC TGTTGATGAC 
CGGGATAGTG ACTATCGCAG TGAGACCAGC AATAGCATCC CACCTCCTTA TCATACAACT 
TCCCAGCCCA ACGCTTCTGT GCACCAGTTT CCTGTGCCCA TGCGATTGCC ACAGCAGCTG 
CTACTTCAGG GCAATTCCCG GGATTCTTGC AATGACTCCA TGCAAAGTTA CGATCTTGAT 
TATCCAGAGC GGCGGGCCCT TAGCCCCACC AGCAGTAGTA GGTATGGCTC ATCCTGTAAT 
GTGAGTCAAG GAAGCTCTCA GTTGAGTGAA CTAGACCAGT GTCACGAACA AGATGATGAC 
CGTCGGGAGA GGGATTCAAT TCATTCATGC CACAGCTCTG GCAGCTTCTC CAGAGACGGC 
CAGGTGGGCT TTGGAGAACA AGAGAAAGCC TTGGAGGTAA CAGGTGAAGA AGAGAAGGGG 
AGAGTGTGTG AACCCAAGGA CAAGAAAAAA GATACCACAA CCCATCTTCC CCCAGATCTG 
GTGCTACCCA AAGACCACGT CCTAGGCCCC CAGGAGAGTT TTCCTGAGGA GAATGGTTCT 
TCACCATTTA CCCAAGCAAG AGCTCACTGG ATCCGAGCAG TTACCAAGGT TCGACTCCAG 
CTGCAGGAGA TTCCGGATGA TGGTCACCCT TCTTTGCCCC AGTGGCTTCC GGAAGGGCCT 
GCTGGAGGGC TCTATGGCAT TGACAGCATG CCAGATCTAC GCAGAAAGAA ACCATTGCCA 
CTTGTCAGTG ATCTGGCTAT GTCATTGGTC CAGTCCCGGA AAGCAGGAAT TACTTCTGCA 
ATGGCTACAC GTACCTCTCT GAAGGATGAA GAGCTGAAAT CCCATGTGTA TAAGAAAACC 
CTGCAGGCCT TAATCTACCC AATCTCGTGC ACCACACCCC ACAACTTTGA AGTCTGGACA 
GCCACTACCC CAACCTACTG CTATGAGTGT GAAGGTCTGC TCTGGGGCAT TGCCCGGCAG 
GGCATGCGCT GCAGCGAGTG TGGAGTCAAG TGCCATGAGA AGTGCCAAGA CCTGCTCAAC 
GCCGACTGCC TGCAGCGGGC TGCAGAAAAG AGTTCTAAAC ATGGAGCTGA GGACCGGACC 
CAGAACATAA TCATGGCGAT GAAGGACCGC ATGAAGATCC GAGAGCGGAA TAAGCCAGAG 
ATCTTTGAGG TTATCCGAGA TGTCTTTACC GTGAGCAAAG TTGCCCATGT GCAACAGATG 
AAAACAGTGA AGCAGAGTGT ACTGGATGGC ACCTCCAAAT GGTCAGCCAA AATTACCATC 
ACTGTGGTGT GTGCCCAGGG CCTACAAGCC AAGGACAAGA CAGGATCCAG TGACCCTTAT 
GTGACTGTGC AAGTGGGCAA AACCAAGAAG CGTACCAAGA CTATTTTTGG GAACCTGAAT 
CCTGTTTGGG AAGAGAAGTT CCATTTTGAG TGCCACAACT CTTCTGACCG CATTAAGGTA 
CGTGTATGGG ATGAGGATGA TGACATCAAG TCAAGAGTAA AGCAACGGCT AAAGCGAGAG 
TCCGATGATT TCCTTGGCCA AACCATTATT GAGGTTCGGA CACTGAGTGG CGAGATGGAC 
GTCTGGTACA ACTTGGAGAA GAGGACAGAT AAATCAGCTG TCTCAGGGGC TATCCGACTG 
CAAATCAGTG TGGAGATCAA GGGAGAGGAG AAAGTAGCGC CATATCATGT GCAGTATACG 
TGTCTCCATG AGAACCTTTT CCATTACCTC ACAGACATTC AGGGTAGTGG AGGAGTCCTG 
ATCCCTGAGG CTCGCGGAGA CGATGCATGG AAGGTGTACT TTGATGAGAC TGCCCAAGAA 
ATTGTGGATG AATTTGCCAT GCGCTATGGC ATCGAGTCCA TATATCAGGC CATGACGCAC 
TTTGCATGTT TGTCATCCAA GTACATGTGT CCTGGTGTGC CTGCGGTGAT GAGCACCTTA 
TTGGCCAACA TCAATGCCTA CTATGCCCAT ACAACTGCCT CTACCAACGT CTCTGCATCT 
GATCGCTTTG CAGCCTCCAA CTTTGGGAAA GAGAGATTTG TAAAACTACT GGACCAGCTG 
CACAACTCAC TGAGGATTGA CCTCTCTACC TACAGGAATA ATTTCCCTGC TGGAAGCCCT 
GAGCGGCTTC AGGACTTAAA GTCAACAGTG GATTTGCTGA CCAGCATTAC TTTCTTCAGA 
ATGAAGGTAC AAGAACTGCA GAGCCCTCCA AGAGCCAGCC AAGTGGTAAA GGATTGTGTG 
AAGGCCTGTT TGAACTCCAC ATATGAGTAT ATCTTCAACA ACTGCCATGA CTTGTATAGT 
CGCCAGTACC AGCTGAAACA GGAGCTACCT CCAGAGGAAC AAGGACCCAG CATTCGGAAC 
CTGGATTTTT GGCCCAAACT CATCACGCTC ATTGTGTCAA TCATAGAGGA AGATAAGAAT 
TCCTACACAC TCGTTCTGAA CCAGTTTCCT CAGGAATTGA ATGTGGGAAA AGTCAGCGCA 
GAAGTGATGT GGCAGCTCTT TGCCCAAGAC ATGAAATATG CACTGGAGGA GCATGAGAAG 
GACCGGCTGT GTAAGAGTGC TGACTACATG AACCTGCACT TCAAGGTGAA GTGGCTCCAC 
AATGAATACG TGCGAGATCT GCCTGCCCTG CAGGGGCAGG TGCCTGAGTA CCCAGTGTGG 
TTTGAGCAGT TTGTTCTGCA ATGGCTGGAT GAAAATGAGG ATGTGTCCCT GGAATTCCTG 
CGTGGGGCTC TGGAACGAGA TAAGAAGGAT GGGTTCCAGC AGACATCAGA GCATGCACTC 
TTTTCCTGCT CTGTGGTGGA TGTCTTCACA CAGCTCAACC AGAGCTTTGA GATCATCCGG 
AAGCTGGAGT GCCCAGACCC CAACATCCTT GCCCACTACA TGAGGAGATT TGCTAAGACC 
ATCGGGAAGG TGTTGATGCA GTATGCAAAC ATCTTATCAA AGAGCTTCCC AGATTATTGC 
ACAAAGGAGA AAATGCCCTG CATCCTGATG AACAACATGC AGCAACTGAG GGTCCAGCTG 
GAGAAAATGT TTGAGGCCAT GGGAGGCAAG GAGTTGGACC CTGAAGCTGC AGACAGTTTG 
AAGGAGCTGC AGGTGAAACT GAATACAGTT CTGGATGAGC TCAGCATGGT ATTTGGAAAC 
AGTTTTCAGG TGCGGATTGA TGAGTGTGTT CGACAAATGG CTGACATCCT GGGCCAGGTG 
CGAGGCCCAG GGAATGCATC CCCCAACGCC AGGGCCTCTG TGGCTCAGGA TGCAGATAGT 
GTGCTCCGGC CTCTCATGGA CTTCCTGGAT GGCAACCTCA CACTCTTTGC CACTGTGTGT 
GAGAAGACAG TCCTGAAGCG TGTACTGAAG GAGCTCTGGA GGGTGGTCAT GAACACAATG 
GAAAGAATGA TTGTTCTGCC CCCACTCACT GACCAGACGG GCACCCAGCT GATCTTTACT 
GCTGCCAAGG AGCTGAGCCA TCTTTCTAAG CTCAAGGACC ACATGGTGCG AGAGGAAACA 
CGGAGTCTCA CTCCAAAGCA GTGTGAAGTC CTTGACCTCG CTCTGGACAC TATCAAGCAA 
TACTTTCATG CAGGAGGTAA TGGGCTGAAG AAAACCTTCC TGGAGAAGAG CTCAGATCTG 
CAGTCTCTAC GCTATGCCCT GTCTCTCTAC ACACAGACCA CAGATACACT CATCAAAACC 
TTTGTACGCT CACAGACTGC CCAGGTGCAT GATGGGAAAG GTATTAGGTT TACTGCTAAT 
GAGGACATTC GGCCGGAAAA GGGATCTGGT GTGGATGATC CTGTGGGAGA AGTCTCTATT 
CAGGTGGACT TGTTTACACA TCCTGGAACT GGGGAGCACA AGGTCACAGT GAAAGTGGTA 
GCTGCCAATG ACCTCAAGTG GCAGACAGCG GGTATGTTCC GGCCCTTTGT GGAAGTGACC 
ATGGTTGGCC CACACCAAAG TGAAAAGAAG AGGAAGTTCA CAACCAAGTC AAAAAGCAAC 
AACTGGGCTC CCAAGTACAA TGAGACATTT CACTTCCTCC TGGGAAATGA AGAAGGGCCA 
GAAGCCTATG AATTGCAAAT ATGTGTGAAG GATTACTGCT TTGCCCGTGA GGATCGTGTT 
TTGGGGCTGG CTGTGATGCC TCTGAGGGAT GTAGCAGCCA AGGGCAGCTG TGCTTGCTGG 
TGTCCCTTGG GCCGGAAGAT CCACATGGAT GAGACAGGCA TGACTATTCT CCGGATTCTA 
TCTCAAAGGA GCAATGACGA AGTGGCTCGA GAGTTTGTGA AGCTCAAATC GGAGTCTCGT 
TCCATGGAAG AGGGGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117831.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973306.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X7, mRNA.

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CloneID	OCe39188
Clone ID Related Accession (Same CDS sequence)	XM_014117831.1
Accession Version	XM_014117831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTCT TTTGAAGATC CTGATAGTGC TGTTGATGAC 
CGGGATAGTG ACTATCGCAG TGAGACCAGC AATAGCATCC CACCTCCTTA TCATACAACT 
TCCCAGCCCA ACGCTTCTGT GCACCAGTTT CCTGTGCCCA TGCGATTGCC ACAGCAGCTG 
CTACTTCAGG GCAATTCCCG GGATTCTTGC AATGACTCCA TGCAAAGTTA CGATCTTGAT 
TATCCAGAGC GGCGGGCCCT TAGCCCCACC AGCAGTAGTA GGTATGGCTC ATCCTGTAAT 
GTGAGTCAAG GAAGCTCTCA GTTGAGTGAA CTAGACCAGT GTCACGAACA AGATGATGAC 
CGTCGGGAGA GGGATTCAAT TCATTCATGC CACAGCTCTG GCAGCTTCTC CAGAGACGGC 
CAGGTGGGCT TTGGAGAACA AGAGAAAGCC TTGGAGGTAA CAGGTGAAGA AGAGAAGGGG 
AGAGTGTGTG AACCCAAGGA CAAGAAAAAA GATACCACAA CCCATCTTCC CCCAGATCTG 
GTGCTACCCA AAGACCACGT CCTAGGCCCC CAGGAGAGTT TTCCTGAGGA GAATGGTTCT 
TCACCATTTA CCCAAGCAAG AGCTCACTGG ATCCGAGCAG TTACCAAGGT TCGACTCCAG 
CTGCAGGAGA TTCCGGATGA TGGTCACCCT TCTTTGCCCC AGTGGCTTCC GGAAGGGCCT 
GCTGGAGGGC TCTATGGCAT TGACAGCATG CCAGATCTAC GCAGAAAGAA ACCATTGCCA 
CTTGTCAGTG ATCTGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGT GCATGATGGG AAAGGTATTA GGTTTACTGC TAATGAGGAC 
ATTCGGCCGG AAAAGGGATC TGGTGTGGAT GATCCTGTGG GAGAAGTCTC TATTCAGGTG 
GACTTGTTTA CACATCCTGG AACTGGGGAG CACAAGGTCA CAGTGAAAGT GGTAGCTGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCCT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGAAAA GAAGAGGAAG TTCACAACCA AGTCAAAAAG CAACAACTGG 
GCTCCCAAGT ACAATGAGAC ATTTCACTTC CTCCTGGGAA ATGAAGAAGG GCCAGAAGCC 
TATGAATTGC AAATATGTGT GAAGGATTAC TGCTTTGCCC GTGAGGATCG TGTTTTGGGG 
CTGGCTGTGA TGCCTCTGAG GGATGTAGCA GCCAAGGGCA GCTGTGCTTG CTGGTGTCCC 
TTGGGCCGGA AGATCCACAT GGATGAGACA GGCATGACTA TTCTCCGGAT TCTATCTCAA 
AGGAGCAATG ACGAAGTGGC TCGAGAGTTT GTGAAGCTCA AATCGGAGTC TCGTTCCATG 
GAAGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973306.1
CDS	17..5011
Translation	MGEGCGVCKVVRGLAGVCEDATIWLLGSQVCEVEQMSAAYAVQENLSSGRWAMTARSLPLEVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_014117831.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117831.1

ORF Insert Sequence:

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTCT TTTGAAGATC CTGATAGTGC TGTTGATGAC 
CGGGATAGTG ACTATCGCAG TGAGACCAGC AATAGCATCC CACCTCCTTA TCATACAACT 
TCCCAGCCCA ACGCTTCTGT GCACCAGTTT CCTGTGCCCA TGCGATTGCC ACAGCAGCTG 
CTACTTCAGG GCAATTCCCG GGATTCTTGC AATGACTCCA TGCAAAGTTA CGATCTTGAT 
TATCCAGAGC GGCGGGCCCT TAGCCCCACC AGCAGTAGTA GGTATGGCTC ATCCTGTAAT 
GTGAGTCAAG GAAGCTCTCA GTTGAGTGAA CTAGACCAGT GTCACGAACA AGATGATGAC 
CGTCGGGAGA GGGATTCAAT TCATTCATGC CACAGCTCTG GCAGCTTCTC CAGAGACGGC 
CAGGTGGGCT TTGGAGAACA AGAGAAAGCC TTGGAGGTAA CAGGTGAAGA AGAGAAGGGG 
AGAGTGTGTG AACCCAAGGA CAAGAAAAAA GATACCACAA CCCATCTTCC CCCAGATCTG 
GTGCTACCCA AAGACCACGT CCTAGGCCCC CAGGAGAGTT TTCCTGAGGA GAATGGTTCT 
TCACCATTTA CCCAAGCAAG AGCTCACTGG ATCCGAGCAG TTACCAAGGT TCGACTCCAG 
CTGCAGGAGA TTCCGGATGA TGGTCACCCT TCTTTGCCCC AGTGGCTTCC GGAAGGGCCT 
GCTGGAGGGC TCTATGGCAT TGACAGCATG CCAGATCTAC GCAGAAAGAA ACCATTGCCA 
CTTGTCAGTG ATCTGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGT GCATGATGGG AAAGGTATTA GGTTTACTGC TAATGAGGAC 
ATTCGGCCGG AAAAGGGATC TGGTGTGGAT GATCCTGTGG GAGAAGTCTC TATTCAGGTG 
GACTTGTTTA CACATCCTGG AACTGGGGAG CACAAGGTCA CAGTGAAAGT GGTAGCTGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCCT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGAAAA GAAGAGGAAG TTCACAACCA AGTCAAAAAG CAACAACTGG 
GCTCCCAAGT ACAATGAGAC ATTTCACTTC CTCCTGGGAA ATGAAGAAGG GCCAGAAGCC 
TATGAATTGC AAATATGTGT GAAGGATTAC TGCTTTGCCC GTGAGGATCG TGTTTTGGGG 
CTGGCTGTGA TGCCTCTGAG GGATGTAGCA GCCAAGGGCA GCTGTGCTTG CTGGTGTCCC 
TTGGGCCGGA AGATCCACAT GGATGAGACA GGCATGACTA TTCTCCGGAT TCTATCTCAA 
AGGAGCAATG ACGAAGTGGC TCGAGAGTTT GTGAAGCTCA AATCGGAGTC TCGTTCCATG 
GAAGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117833.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973308.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X8, mRNA.

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CloneID	OCe39189
Clone ID Related Accession (Same CDS sequence)	XM_014117833.1
Accession Version	XM_014117833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTGT CACTGGACCT ATTTGGGCTG GGGAGAGCAG 
CAGACTTTTG AAGATCCTGA TAGTGCTGTT GATGACCGGG ATAGTGACTA TCGCAGTGAG 
ACCAGCAATA GCATCCCACC TCCTTATCAT ACAACTTCCC AGCCCAACGC TTCTGTGCAC 
CAGTTTCCTG TGCCCATGCG ATTGCCACAG CAGCTGCTAC TTCAGGGCAA TTCCCGGGAT 
TCTTGCAATG ACTCCATGCA AAGTTACGAT CTTGATTATC CAGAGCGGCG GGCCCTTAGC 
CCCACCAGCA GTAGTAGGTA TGGCTCATCC TGTAATGTGA GTCAAGGAAG CTCTCAGTTG 
AGTGAACTAG ACCAGTGTCA CGAACAAGAT GATGACCGTC GGGAGAGGGA TTCAATTCAT 
TCATGCCACA GCTCTGGCAG CTTCTCCAGA GACGGCCAGG TGGGCTTTGG AGAACAAGAG 
AAAGCCTTGG AGGTAACAGG TGAAGAAGAG AAGGGGAGAG TGTGTGAACC CAAGGACAAG 
AAAAAAGATA CCACAACCCA TCTTCCCCCA GATCTGGTGC TACCCAAAGA CCACGTCCTA 
GGCCCCCAGG AGAGTTTTCC TGAGGAGAAT GGTTCTTCAC CATTTACCCA AGCAAGAGCT 
CACTGGATCC GAGCAGTTAC CAAGGTTCGA CTCCAGCTGC AGGAGATTCC GGATGATGGT 
CACCCTTCTT TGCCCCAGTG GCTTCCGGAA GGGCCTGCTG GAGGGCTCTA TGGCATTGAC 
AGCATGCCAG ATCTACGCAG AAAGAAACCA TTGCCACTTG TCAGTGATCT GTCATTGGTC 
CAGTCCCGGA AAGCAGGAAT TACTTCTGCA ATGGCTACAC GTACCTCTCT GAAGGATGAA 
GAGCTGAAAT CCCATGTGTA TAAGAAAACC CTGCAGGCCT TAATCTACCC AATCTCGTGC 
ACCACACCCC ACAACTTTGA AGTCTGGACA GCCACTACCC CAACCTACTG CTATGAGTGT 
GAAGGTCTGC TCTGGGGCAT TGCCCGGCAG GGCATGCGCT GCAGCGAGTG TGGAGTCAAG 
TGCCATGAGA AGTGCCAAGA CCTGCTCAAC GCCGACTGCC TGCAGCGGGC TGCAGAAAAG 
AGTTCTAAAC ATGGAGCTGA GGACCGGACC CAGAACATAA TCATGGCGAT GAAGGACCGC 
ATGAAGATCC GAGAGCGGAA TAAGCCAGAG ATCTTTGAGG TTATCCGAGA TGTCTTTACC 
GTGAGCAAAG TTGCCCATGT GCAACAGATG AAAACAGTGA AGCAGAGTGT ACTGGATGGC 
ACCTCCAAAT GGTCAGCCAA AATTACCATC ACTGTGGTGT GTGCCCAGGG CCTACAAGCC 
AAGGACAAGA CAGGATCCAG TGACCCTTAT GTGACTGTGC AAGTGGGCAA AACCAAGAAG 
CGTACCAAGA CTATTTTTGG GAACCTGAAT CCTGTTTGGG AAGAGAAGTT CCATTTTGAG 
TGCCACAACT CTTCTGACCG CATTAAGGTA CGTGTATGGG ATGAGGATGA TGACATCAAG 
TCAAGAGTAA AGCAACGGCT AAAGCGAGAG TCCGATGATT TCCTTGGCCA AACCATTATT 
GAGGTTCGGA CACTGAGTGG CGAGATGGAC GTCTGGTACA ACTTGGAGAA GAGGACAGAT 
AAATCAGCTG TCTCAGGGGC TATCCGACTG CAAATCAGTG TGGAGATCAA GGGAGAGGAG 
AAAGTAGCGC CATATCATGT GCAGTATACG TGTCTCCATG AGAACCTTTT CCATTACCTC 
ACAGACATTC AGGGTAGTGG AGGAGTCCTG ATCCCTGAGG CTCGCGGAGA CGATGCATGG 
AAGGTGTACT TTGATGAGAC TGCCCAAGAA ATTGTGGATG AATTTGCCAT GCGCTATGGC 
ATCGAGTCCA TATATCAGGC CATGACGCAC TTTGCATGTT TGTCATCCAA GTACATGTGT 
CCTGGTGTGC CTGCGGTGAT GAGCACCTTA TTGGCCAACA TCAATGCCTA CTATGCCCAT 
ACAACTGCCT CTACCAACGT CTCTGCATCT GATCGCTTTG CAGCCTCCAA CTTTGGGAAA 
GAGAGATTTG TAAAACTACT GGACCAGCTG CACAACTCAC TGAGGATTGA CCTCTCTACC 
TACAGGAATA ATTTCCCTGC TGGAAGCCCT GAGCGGCTTC AGGACTTAAA GTCAACAGTG 
GATTTGCTGA CCAGCATTAC TTTCTTCAGA ATGAAGGTAC AAGAACTGCA GAGCCCTCCA 
AGAGCCAGCC AAGTGGTAAA GGATTGTGTG AAGGCCTGTT TGAACTCCAC ATATGAGTAT 
ATCTTCAACA ACTGCCATGA CTTGTATAGT CGCCAGTACC AGCTGAAACA GGAGCTACCT 
CCAGAGGAAC AAGGACCCAG CATTCGGAAC CTGGATTTTT GGCCCAAACT CATCACGCTC 
ATTGTGTCAA TCATAGAGGA AGATAAGAAT TCCTACACAC TCGTTCTGAA CCAGTTTCCT 
CAGGAATTGA ATGTGGGAAA AGTCAGCGCA GAAGTGATGT GGCAGCTCTT TGCCCAAGAC 
ATGAAATATG CACTGGAGGA GCATGAGAAG GACCGGCTGT GTAAGAGTGC TGACTACATG 
AACCTGCACT TCAAGGTGAA GTGGCTCCAC AATGAATACG TGCGAGATCT GCCTGCCCTG 
CAGGGGCAGG TGCCTGAGTA CCCAGTGTGG TTTGAGCAGT TTGTTCTGCA ATGGCTGGAT 
GAAAATGAGG ATGTGTCCCT GGAATTCCTG CGTGGGGCTC TGGAACGAGA TAAGAAGGAT 
GGGTTCCAGC AGACATCAGA GCATGCACTC TTTTCCTGCT CTGTGGTGGA TGTCTTCACA 
CAGCTCAACC AGAGCTTTGA GATCATCCGG AAGCTGGAGT GCCCAGACCC CAACATCCTT 
GCCCACTACA TGAGGAGATT TGCTAAGACC ATCGGGAAGG TGTTGATGCA GTATGCAAAC 
ATCTTATCAA AGAGCTTCCC AGATTATTGC ACAAAGGAGA AAATGCCCTG CATCCTGATG 
AACAACATGC AGCAACTGAG GGTCCAGCTG GAGAAAATGT TTGAGGCCAT GGGAGGCAAG 
GAGTTGGACC CTGAAGCTGC AGACAGTTTG AAGGAGCTGC AGGTGAAACT GAATACAGTT 
CTGGATGAGC TCAGCATGGT ATTTGGAAAC AGTTTTCAGG TGCGGATTGA TGAGTGTGTT 
CGACAAATGG CTGACATCCT GGGCCAGGTG CGAGGCCCAG GGAATGCATC CCCCAACGCC 
AGGGCCTCTG TGGCTCAGGA TGCAGATAGT GTGCTCCGGC CTCTCATGGA CTTCCTGGAT 
GGCAACCTCA CACTCTTTGC CACTGTGTGT GAGAAGACAG TCCTGAAGCG TGTACTGAAG 
GAGCTCTGGA GGGTGGTCAT GAACACAATG GAAAGAATGA TTGTTCTGCC CCCACTCACT 
GACCAGACGG GCACCCAGCT GATCTTTACT GCTGCCAAGG AGCTGAGCCA TCTTTCTAAG 
CTCAAGGACC ACATGGTGCG AGAGGAAACA CGGAGTCTCA CTCCAAAGCA GTGTGAAGTC 
CTTGACCTCG CTCTGGACAC TATCAAGCAA TACTTTCATG CAGGAGGTAA TGGGCTGAAG 
AAAACCTTCC TGGAGAAGAG CTCAGATCTG CAGTCTCTAC GCTATGCCCT GTCTCTCTAC 
ACACAGACCA CAGATACACT CATCAAAACC TTTGTACGCT CACAGACTGC CCAGGGATCT 
GGTGTGGATG ATCCTGTGGG AGAAGTCTCT ATTCAGGTGG ACTTGTTTAC ACATCCTGGA 
ACTGGGGAGC ACAAGGTCAC AGTGAAAGTG GTAGCTGCCA ATGACCTCAA GTGGCAGACA 
GCGGGTATGT TCCGGCCCTT TGTGGAAGTG ACCATGGTTG GCCCACACCA AAGTGAAAAG 
AAGAGGAAGT TCACAACCAA GTCAAAAAGC AACAACTGGG CTCCCAAGTA CAATGAGACA 
TTTCACTTCC TCCTGGGAAA TGAAGAAGGG CCAGAAGCCT ATGAATTGCA AATATGTGTG 
AAGGATTACT GCTTTGCCCG TGAGGATCGT GTTTTGGGGC TGGCTGTGAT GCCTCTGAGG 
GATGTAGCAG CCAAGGGCAG CTGTGCTTGC TGGTGTCCCT TGGGCCGGAA GATCCACATG 
GATGAGACAG GCATGACTAT TCTCCGGATT CTATCTCAAA GGAGCAATGA CGAAGTGGCT 
CGAGAGTTTG TGAAGCTCAA ATCGGAGTCT CGTTCCATGG AAGAGGGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973308.1
CDS	17..4990
Translation	MGEGCGVCKVVRGLAGVCEDATIWLLGSQVCEVEQMSAAYAVQENLSSGRWAMTARSLPLEVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCCHWTYLGWGEQQTFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_014117833.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117833.1

ORF Insert Sequence:

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTGT CACTGGACCT ATTTGGGCTG GGGAGAGCAG 
CAGACTTTTG AAGATCCTGA TAGTGCTGTT GATGACCGGG ATAGTGACTA TCGCAGTGAG 
ACCAGCAATA GCATCCCACC TCCTTATCAT ACAACTTCCC AGCCCAACGC TTCTGTGCAC 
CAGTTTCCTG TGCCCATGCG ATTGCCACAG CAGCTGCTAC TTCAGGGCAA TTCCCGGGAT 
TCTTGCAATG ACTCCATGCA AAGTTACGAT CTTGATTATC CAGAGCGGCG GGCCCTTAGC 
CCCACCAGCA GTAGTAGGTA TGGCTCATCC TGTAATGTGA GTCAAGGAAG CTCTCAGTTG 
AGTGAACTAG ACCAGTGTCA CGAACAAGAT GATGACCGTC GGGAGAGGGA TTCAATTCAT 
TCATGCCACA GCTCTGGCAG CTTCTCCAGA GACGGCCAGG TGGGCTTTGG AGAACAAGAG 
AAAGCCTTGG AGGTAACAGG TGAAGAAGAG AAGGGGAGAG TGTGTGAACC CAAGGACAAG 
AAAAAAGATA CCACAACCCA TCTTCCCCCA GATCTGGTGC TACCCAAAGA CCACGTCCTA 
GGCCCCCAGG AGAGTTTTCC TGAGGAGAAT GGTTCTTCAC CATTTACCCA AGCAAGAGCT 
CACTGGATCC GAGCAGTTAC CAAGGTTCGA CTCCAGCTGC AGGAGATTCC GGATGATGGT 
CACCCTTCTT TGCCCCAGTG GCTTCCGGAA GGGCCTGCTG GAGGGCTCTA TGGCATTGAC 
AGCATGCCAG ATCTACGCAG AAAGAAACCA TTGCCACTTG TCAGTGATCT GTCATTGGTC 
CAGTCCCGGA AAGCAGGAAT TACTTCTGCA ATGGCTACAC GTACCTCTCT GAAGGATGAA 
GAGCTGAAAT CCCATGTGTA TAAGAAAACC CTGCAGGCCT TAATCTACCC AATCTCGTGC 
ACCACACCCC ACAACTTTGA AGTCTGGACA GCCACTACCC CAACCTACTG CTATGAGTGT 
GAAGGTCTGC TCTGGGGCAT TGCCCGGCAG GGCATGCGCT GCAGCGAGTG TGGAGTCAAG 
TGCCATGAGA AGTGCCAAGA CCTGCTCAAC GCCGACTGCC TGCAGCGGGC TGCAGAAAAG 
AGTTCTAAAC ATGGAGCTGA GGACCGGACC CAGAACATAA TCATGGCGAT GAAGGACCGC 
ATGAAGATCC GAGAGCGGAA TAAGCCAGAG ATCTTTGAGG TTATCCGAGA TGTCTTTACC 
GTGAGCAAAG TTGCCCATGT GCAACAGATG AAAACAGTGA AGCAGAGTGT ACTGGATGGC 
ACCTCCAAAT GGTCAGCCAA AATTACCATC ACTGTGGTGT GTGCCCAGGG CCTACAAGCC 
AAGGACAAGA CAGGATCCAG TGACCCTTAT GTGACTGTGC AAGTGGGCAA AACCAAGAAG 
CGTACCAAGA CTATTTTTGG GAACCTGAAT CCTGTTTGGG AAGAGAAGTT CCATTTTGAG 
TGCCACAACT CTTCTGACCG CATTAAGGTA CGTGTATGGG ATGAGGATGA TGACATCAAG 
TCAAGAGTAA AGCAACGGCT AAAGCGAGAG TCCGATGATT TCCTTGGCCA AACCATTATT 
GAGGTTCGGA CACTGAGTGG CGAGATGGAC GTCTGGTACA ACTTGGAGAA GAGGACAGAT 
AAATCAGCTG TCTCAGGGGC TATCCGACTG CAAATCAGTG TGGAGATCAA GGGAGAGGAG 
AAAGTAGCGC CATATCATGT GCAGTATACG TGTCTCCATG AGAACCTTTT CCATTACCTC 
ACAGACATTC AGGGTAGTGG AGGAGTCCTG ATCCCTGAGG CTCGCGGAGA CGATGCATGG 
AAGGTGTACT TTGATGAGAC TGCCCAAGAA ATTGTGGATG AATTTGCCAT GCGCTATGGC 
ATCGAGTCCA TATATCAGGC CATGACGCAC TTTGCATGTT TGTCATCCAA GTACATGTGT 
CCTGGTGTGC CTGCGGTGAT GAGCACCTTA TTGGCCAACA TCAATGCCTA CTATGCCCAT 
ACAACTGCCT CTACCAACGT CTCTGCATCT GATCGCTTTG CAGCCTCCAA CTTTGGGAAA 
GAGAGATTTG TAAAACTACT GGACCAGCTG CACAACTCAC TGAGGATTGA CCTCTCTACC 
TACAGGAATA ATTTCCCTGC TGGAAGCCCT GAGCGGCTTC AGGACTTAAA GTCAACAGTG 
GATTTGCTGA CCAGCATTAC TTTCTTCAGA ATGAAGGTAC AAGAACTGCA GAGCCCTCCA 
AGAGCCAGCC AAGTGGTAAA GGATTGTGTG AAGGCCTGTT TGAACTCCAC ATATGAGTAT 
ATCTTCAACA ACTGCCATGA CTTGTATAGT CGCCAGTACC AGCTGAAACA GGAGCTACCT 
CCAGAGGAAC AAGGACCCAG CATTCGGAAC CTGGATTTTT GGCCCAAACT CATCACGCTC 
ATTGTGTCAA TCATAGAGGA AGATAAGAAT TCCTACACAC TCGTTCTGAA CCAGTTTCCT 
CAGGAATTGA ATGTGGGAAA AGTCAGCGCA GAAGTGATGT GGCAGCTCTT TGCCCAAGAC 
ATGAAATATG CACTGGAGGA GCATGAGAAG GACCGGCTGT GTAAGAGTGC TGACTACATG 
AACCTGCACT TCAAGGTGAA GTGGCTCCAC AATGAATACG TGCGAGATCT GCCTGCCCTG 
CAGGGGCAGG TGCCTGAGTA CCCAGTGTGG TTTGAGCAGT TTGTTCTGCA ATGGCTGGAT 
GAAAATGAGG ATGTGTCCCT GGAATTCCTG CGTGGGGCTC TGGAACGAGA TAAGAAGGAT 
GGGTTCCAGC AGACATCAGA GCATGCACTC TTTTCCTGCT CTGTGGTGGA TGTCTTCACA 
CAGCTCAACC AGAGCTTTGA GATCATCCGG AAGCTGGAGT GCCCAGACCC CAACATCCTT 
GCCCACTACA TGAGGAGATT TGCTAAGACC ATCGGGAAGG TGTTGATGCA GTATGCAAAC 
ATCTTATCAA AGAGCTTCCC AGATTATTGC ACAAAGGAGA AAATGCCCTG CATCCTGATG 
AACAACATGC AGCAACTGAG GGTCCAGCTG GAGAAAATGT TTGAGGCCAT GGGAGGCAAG 
GAGTTGGACC CTGAAGCTGC AGACAGTTTG AAGGAGCTGC AGGTGAAACT GAATACAGTT 
CTGGATGAGC TCAGCATGGT ATTTGGAAAC AGTTTTCAGG TGCGGATTGA TGAGTGTGTT 
CGACAAATGG CTGACATCCT GGGCCAGGTG CGAGGCCCAG GGAATGCATC CCCCAACGCC 
AGGGCCTCTG TGGCTCAGGA TGCAGATAGT GTGCTCCGGC CTCTCATGGA CTTCCTGGAT 
GGCAACCTCA CACTCTTTGC CACTGTGTGT GAGAAGACAG TCCTGAAGCG TGTACTGAAG 
GAGCTCTGGA GGGTGGTCAT GAACACAATG GAAAGAATGA TTGTTCTGCC CCCACTCACT 
GACCAGACGG GCACCCAGCT GATCTTTACT GCTGCCAAGG AGCTGAGCCA TCTTTCTAAG 
CTCAAGGACC ACATGGTGCG AGAGGAAACA CGGAGTCTCA CTCCAAAGCA GTGTGAAGTC 
CTTGACCTCG CTCTGGACAC TATCAAGCAA TACTTTCATG CAGGAGGTAA TGGGCTGAAG 
AAAACCTTCC TGGAGAAGAG CTCAGATCTG CAGTCTCTAC GCTATGCCCT GTCTCTCTAC 
ACACAGACCA CAGATACACT CATCAAAACC TTTGTACGCT CACAGACTGC CCAGGGATCT 
GGTGTGGATG ATCCTGTGGG AGAAGTCTCT ATTCAGGTGG ACTTGTTTAC ACATCCTGGA 
ACTGGGGAGC ACAAGGTCAC AGTGAAAGTG GTAGCTGCCA ATGACCTCAA GTGGCAGACA 
GCGGGTATGT TCCGGCCCTT TGTGGAAGTG ACCATGGTTG GCCCACACCA AAGTGAAAAG 
AAGAGGAAGT TCACAACCAA GTCAAAAAGC AACAACTGGG CTCCCAAGTA CAATGAGACA 
TTTCACTTCC TCCTGGGAAA TGAAGAAGGG CCAGAAGCCT ATGAATTGCA AATATGTGTG 
AAGGATTACT GCTTTGCCCG TGAGGATCGT GTTTTGGGGC TGGCTGTGAT GCCTCTGAGG 
GATGTAGCAG CCAAGGGCAG CTGTGCTTGC TGGTGTCCCT TGGGCCGGAA GATCCACATG 
GATGAGACAG GCATGACTAT TCTCCGGATT CTATCTCAAA GGAGCAATGA CGAAGTGGCT 
CGAGAGTTTG TGAAGCTCAA ATCGGAGTCT CGTTCCATGG AAGAGGGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117834.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973309.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X9, mRNA.

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CloneID	OCe39190
Clone ID Related Accession (Same CDS sequence)	XM_014117834.1
Accession Version	XM_014117834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGGTCAG TGTCCCCACA CAGTCATAAG AGCCAGCTAT GGCCCCTGGC CAATCCCAAA 
GCCCCTGCTC CACACCCTCC ATCCAGTGCA GGTGTGTGGA TCTTCCCTTT CCTCCTGTTG 
GCTGTCCCTG CTGGAGCTGT ACCAGGAGTG TGCTGGAATG GGCCTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973309.1
CDS	870..5840
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNRSVSPHSHKSQLWPLANPKAPAPHPPSSAGVWIFPFLLLAVPAGAVPGVCWNGPE

Target ORF information:

RefSeq Version	XM_014117834.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117834.1

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGGTCAG TGTCCCCACA CAGTCATAAG AGCCAGCTAT GGCCCCTGGC CAATCCCAAA 
GCCCCTGCTC CACACCCTCC ATCCAGTGCA GGTGTGTGGA TCTTCCCTTT CCTCCTGTTG 
GCTGTCCCTG CTGGAGCTGT ACCAGGAGTG TGCTGGAATG GGCCTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117835.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973310.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X10, mRNA.

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CloneID	OCe39191
Clone ID Related Accession (Same CDS sequence)	XM_014117835.1
Accession Version	XM_014117835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973310.1
CDS	869..5830
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGK

Target ORF information:

RefSeq Version	XM_014117835.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117835.1

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_014117836.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973311.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X11, mRNA.

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CloneID	OCe39192
Clone ID Related Accession (Same CDS sequence)	XM_014117836.1
Accession Version	XM_014117836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTCT TTTGAAGATC CTGATAGTGC TGTTGATGAC 
CGGGATAGTG ACTATCGCAG TGAGACCAGC AATAGCATCC CACCTCCTTA TCATACAACT 
TCCCAGCCCA ACGCTTCTGT GCACCAGTTT CCTGTGCCCA TGCGATTGCC ACAGCAGCTG 
CTACTTCAGG GCAATTCCCG GGATTCTTGC AATGACTCCA TGCAAAGTTA CGATCTTGAT 
TATCCAGAGC GGCGGGCCCT TAGCCCCACC AGCAGTAGTA GGTATGGCTC ATCCTGTAAT 
GTGAGTCAAG GAAGCTCTCA GTTGAGTGAA CTAGACCAGT GTCACGAACA AGATGATGAC 
CGTCGGGAGA GGGATTCAAT TCATTCATGC CACAGCTCTG GCAGCTTCTC CAGAGACGGC 
CAGGTGGGCT TTGGAGAACA AGAGAAAGCC TTGGAGGTAA CAGGTGAAGA AGAGAAGGGG 
AGAGTGTGTG AACCCAAGGA CAAGAAAAAA GATACCACAA CCCATCTTCC CCCAGATCTG 
GTGCTACCCA AAGACCACGT CCTAGGCCCC CAGGAGAGTT TTCCTGAGGA GAATGGTTCT 
TCACCATTTA CCCAAGCAAG AGCTCACTGG ATCCGAGCAG TTACCAAGGT TCGACTCCAG 
CTGCAGGAGA TTCCGGATGA TGGTCACCCT TCTTTGCCCC AGTGGCTTCC GGAAGGGCCT 
GCTGGAGGGC TCTATGGCAT TGACAGCATG CCAGATCTAC GCAGAAAGAA ACCATTGCCA 
CTTGTCAGTG ATCTGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973311.1
CDS	17..4954
Translation	MGEGCGVCKVVRGLAGVCEDATIWLLGSQVCEVEQMSAAYAVQENLSSGRWAMTARSLPLEVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_014117836.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117836.1

ORF Insert Sequence:

ATGGGGGAGG GCTGTGGGGT TTGCAAGGTA GTGCGAGGAC TCGCAGGGGT CTGTGAAGAT 
GCGACAATCT GGTTGTTAGG GTCTCAAGTC TGTGAGGTCG AGCAAATGTC TGCAGCCTAC 
GCTGTGCAGG AGAATCTGAG TTCTGGGAGA TGGGCAATGA CAGCCAGATC TCTACCTTTG 
GAAGTTAAAA GAGCCAAATT CCAGGGTTCA CCAGATAAAT TTAACACATA TGTGACCCTG 
AAAGTGCAGA ATGTGAAGAG CACAACTGTA GCAGTTCGTG GTGATCAACC TTCTTGGGAA 
CAAGATTTTA TGTTTGAGAT TAGTCGCCTG GACCTGGGTC TAAGTGTGGA AGTATGGAAC 
AAAGGACTGA TCTGGGACAC CATGGTTGGG ACTGTGTGGA TTGCACTGAA GACTGTTCGT 
CAGTCGGATG AGGAAGGGCC TGGGGAGTGG TCCACGTTGG AGGCAGAGAC ATTGATGAAA 
GATGATGAGA TCTGTGGAAC TAAAAACCCA ACTCCTCATA AGATTTTGCT TGATACAAGA 
TTTGAGCTGC CTTTTGATAT CCCAGAGGAG GAAGCCAGAT ATTGGACCTA CAAATTGGAA 
CAAATCAATG TCTTGGAAAC TGACAGTGAG TATTCTATTC AAGAAGAAAG CCAGAGGAAG 
CCATTGCCCA CTGCTGCCGC CCAGTGTTCT TTTGAAGATC CTGATAGTGC TGTTGATGAC 
CGGGATAGTG ACTATCGCAG TGAGACCAGC AATAGCATCC CACCTCCTTA TCATACAACT 
TCCCAGCCCA ACGCTTCTGT GCACCAGTTT CCTGTGCCCA TGCGATTGCC ACAGCAGCTG 
CTACTTCAGG GCAATTCCCG GGATTCTTGC AATGACTCCA TGCAAAGTTA CGATCTTGAT 
TATCCAGAGC GGCGGGCCCT TAGCCCCACC AGCAGTAGTA GGTATGGCTC ATCCTGTAAT 
GTGAGTCAAG GAAGCTCTCA GTTGAGTGAA CTAGACCAGT GTCACGAACA AGATGATGAC 
CGTCGGGAGA GGGATTCAAT TCATTCATGC CACAGCTCTG GCAGCTTCTC CAGAGACGGC 
CAGGTGGGCT TTGGAGAACA AGAGAAAGCC TTGGAGGTAA CAGGTGAAGA AGAGAAGGGG 
AGAGTGTGTG AACCCAAGGA CAAGAAAAAA GATACCACAA CCCATCTTCC CCCAGATCTG 
GTGCTACCCA AAGACCACGT CCTAGGCCCC CAGGAGAGTT TTCCTGAGGA GAATGGTTCT 
TCACCATTTA CCCAAGCAAG AGCTCACTGG ATCCGAGCAG TTACCAAGGT TCGACTCCAG 
CTGCAGGAGA TTCCGGATGA TGGTCACCCT TCTTTGCCCC AGTGGCTTCC GGAAGGGCCT 
GCTGGAGGGC TCTATGGCAT TGACAGCATG CCAGATCTAC GCAGAAAGAA ACCATTGCCA 
CTTGTCAGTG ATCTGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626764.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626821.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14570
Clone ID Related Accession (Same CDS sequence)	XM_005626764.3 , XM_005626764.2
Accession Version	XM_005626764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGTG CATGATGGGA AAGGTATTAG GTTTACTGCT 
AATGAGGACA TTCGGCCGGA AAAGGGATCT GGTGTGGATG ATCCTGTGGG AGAAGTCTCT 
ATTCAGGTGG ACTTGTTTAC ACATCCTGGA ACTGGGGAGC ACAAGGTCAC AGTGAAAGTG 
GTAGCTGCCA ATGACCTCAA GTGGCAGACA GCGGGTATGT TCCGGCCCTT TGTGGAAGTG 
ACCATGGTTG GCCCACACCA AAGTGAAAAG AAGAGGAAGT TCACAACCAA GTCAAAAAGC 
AACAACTGGG CTCCCAAGTA CAATGAGACA TTTCACTTCC TCCTGGGAAA TGAAGAAGGG 
CCAGAAGCCT ATGAATTGCA AATATGTGTG AAGGATTACT GCTTTGCCCG TGAGGATCGT 
GTTTTGGGGC TGGCTGTGAT GCCTCTGAGG GATGTAGCAG CCAAGGGCAG CTGTGCTTGC 
TGGTGTCCCT TGGGCCGGAA GATCCACATG GATGAGACAG GCATGACTAT TCTCCGGATT 
CTATCTCAAA GGAGCAATGA CGAAGTGGCT CGAGAGTTTG TGAAGCTCAA ATCGGAGTCT 
CGTTCCATGG AAGAGGGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626821.1
CDS	872..6835
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626764.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626764.3

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGTG CATGATGGGA AAGGTATTAG GTTTACTGCT 
AATGAGGACA TTCGGCCGGA AAAGGGATCT GGTGTGGATG ATCCTGTGGG AGAAGTCTCT 
ATTCAGGTGG ACTTGTTTAC ACATCCTGGA ACTGGGGAGC ACAAGGTCAC AGTGAAAGTG 
GTAGCTGCCA ATGACCTCAA GTGGCAGACA GCGGGTATGT TCCGGCCCTT TGTGGAAGTG 
ACCATGGTTG GCCCACACCA AAGTGAAAAG AAGAGGAAGT TCACAACCAA GTCAAAAAGC 
AACAACTGGG CTCCCAAGTA CAATGAGACA TTTCACTTCC TCCTGGGAAA TGAAGAAGGG 
CCAGAAGCCT ATGAATTGCA AATATGTGTG AAGGATTACT GCTTTGCCCG TGAGGATCGT 
GTTTTGGGGC TGGCTGTGAT GCCTCTGAGG GATGTAGCAG CCAAGGGCAG CTGTGCTTGC 
TGGTGTCCCT TGGGCCGGAA GATCCACATG GATGAGACAG GCATGACTAT TCTCCGGATT 
CTATCTCAAA GGAGCAATGA CGAAGTGGCT CGAGAGTTTG TGAAGCTCAA ATCGGAGTCT 
CGTTCCATGG AAGAGGGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626764.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626821.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X1, mRNA.

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CloneID	OCe14570
Clone ID Related Accession (Same CDS sequence)	XM_005626764.3 , XM_005626764.2
Accession Version	XM_005626764.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGTG CATGATGGGA AAGGTATTAG GTTTACTGCT 
AATGAGGACA TTCGGCCGGA AAAGGGATCT GGTGTGGATG ATCCTGTGGG AGAAGTCTCT 
ATTCAGGTGG ACTTGTTTAC ACATCCTGGA ACTGGGGAGC ACAAGGTCAC AGTGAAAGTG 
GTAGCTGCCA ATGACCTCAA GTGGCAGACA GCGGGTATGT TCCGGCCCTT TGTGGAAGTG 
ACCATGGTTG GCCCACACCA AAGTGAAAAG AAGAGGAAGT TCACAACCAA GTCAAAAAGC 
AACAACTGGG CTCCCAAGTA CAATGAGACA TTTCACTTCC TCCTGGGAAA TGAAGAAGGG 
CCAGAAGCCT ATGAATTGCA AATATGTGTG AAGGATTACT GCTTTGCCCG TGAGGATCGT 
GTTTTGGGGC TGGCTGTGAT GCCTCTGAGG GATGTAGCAG CCAAGGGCAG CTGTGCTTGC 
TGGTGTCCCT TGGGCCGGAA GATCCACATG GATGAGACAG GCATGACTAT TCTCCGGATT 
CTATCTCAAA GGAGCAATGA CGAAGTGGCT CGAGAGTTTG TGAAGCTCAA ATCGGAGTCT 
CGTTCCATGG AAGAGGGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626821.1
CDS	866..6829
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626764.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626764.2

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGTG CATGATGGGA AAGGTATTAG GTTTACTGCT 
AATGAGGACA TTCGGCCGGA AAAGGGATCT GGTGTGGATG ATCCTGTGGG AGAAGTCTCT 
ATTCAGGTGG ACTTGTTTAC ACATCCTGGA ACTGGGGAGC ACAAGGTCAC AGTGAAAGTG 
GTAGCTGCCA ATGACCTCAA GTGGCAGACA GCGGGTATGT TCCGGCCCTT TGTGGAAGTG 
ACCATGGTTG GCCCACACCA AAGTGAAAAG AAGAGGAAGT TCACAACCAA GTCAAAAAGC 
AACAACTGGG CTCCCAAGTA CAATGAGACA TTTCACTTCC TCCTGGGAAA TGAAGAAGGG 
CCAGAAGCCT ATGAATTGCA AATATGTGTG AAGGATTACT GCTTTGCCCG TGAGGATCGT 
GTTTTGGGGC TGGCTGTGAT GCCTCTGAGG GATGTAGCAG CCAAGGGCAG CTGTGCTTGC 
TGGTGTCCCT TGGGCCGGAA GATCCACATG GATGAGACAG GCATGACTAT TCTCCGGATT 
CTATCTCAAA GGAGCAATGA CGAAGTGGCT CGAGAGTTTG TGAAGCTCAA ATCGGAGTCT 
CGTTCCATGG AAGAGGGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626765.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626822.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X2, mRNA.

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CloneID	OCe14571
Clone ID Related Accession (Same CDS sequence)	XM_005626765.2 , XM_005626765.3
Accession Version	XM_005626765.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGTC ATTGGTCCAG TCCCGGAAAG CAGGAATTAC TTCTGCAATG 
GCTACACGTA CCTCTCTGAA GGATGAAGAG CTGAAATCCC ATGTGTATAA GAAAACCCTG 
CAGGCCTTAA TCTACCCAAT CTCGTGCACC ACACCCCACA ACTTTGAAGT CTGGACAGCC 
ACTACCCCAA CCTACTGCTA TGAGTGTGAA GGTCTGCTCT GGGGCATTGC CCGGCAGGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAAGACCT GCTCAACGCC 
GACTGCCTGC AGCGGGCTGC AGAAAAGAGT TCTAAACATG GAGCTGAGGA CCGGACCCAG 
AACATAATCA TGGCGATGAA GGACCGCATG AAGATCCGAG AGCGGAATAA GCCAGAGATC 
TTTGAGGTTA TCCGAGATGT CTTTACCGTG AGCAAAGTTG CCCATGTGCA ACAGATGAAA 
ACAGTGAAGC AGAGTGTACT GGATGGCACC TCCAAATGGT CAGCCAAAAT TACCATCACT 
GTGGTGTGTG CCCAGGGCCT ACAAGCCAAG GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TGGGCAAAAC CAAGAAGCGT ACCAAGACTA TTTTTGGGAA CCTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGACCGCAT TAAGGTACGT 
GTATGGGATG AGGATGATGA CATCAAGTCA AGAGTAAAGC AACGGCTAAA GCGAGAGTCC 
GATGATTTCC TTGGCCAAAC CATTATTGAG GTTCGGACAC TGAGTGGCGA GATGGACGTC 
TGGTACAACT TGGAGAAGAG GACAGATAAA TCAGCTGTCT CAGGGGCTAT CCGACTGCAA 
ATCAGTGTGG AGATCAAGGG AGAGGAGAAA GTAGCGCCAT ATCATGTGCA GTATACGTGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GTAGTGGAGG AGTCCTGATC 
CCTGAGGCTC GCGGAGACGA TGCATGGAAG GTGTACTTTG ATGAGACTGC CCAAGAAATT 
GTGGATGAAT TTGCCATGCG CTATGGCATC GAGTCCATAT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTGCCTG CGGTGATGAG CACCTTATTG 
GCCAACATCA ATGCCTACTA TGCCCATACA ACTGCCTCTA CCAACGTCTC TGCATCTGAT 
CGCTTTGCAG CCTCCAACTT TGGGAAAGAG AGATTTGTAA AACTACTGGA CCAGCTGCAC 
AACTCACTGA GGATTGACCT CTCTACCTAC AGGAATAATT TCCCTGCTGG AAGCCCTGAG 
CGGCTTCAGG ACTTAAAGTC AACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGAATG 
AAGGTACAAG AACTGCAGAG CCCTCCAAGA GCCAGCCAAG TGGTAAAGGA TTGTGTGAAG 
GCCTGTTTGA ACTCCACATA TGAGTATATC TTCAACAACT GCCATGACTT GTATAGTCGC 
CAGTACCAGC TGAAACAGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626822.1
CDS	866..6823
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626765.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626765.2

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGTC ATTGGTCCAG TCCCGGAAAG CAGGAATTAC TTCTGCAATG 
GCTACACGTA CCTCTCTGAA GGATGAAGAG CTGAAATCCC ATGTGTATAA GAAAACCCTG 
CAGGCCTTAA TCTACCCAAT CTCGTGCACC ACACCCCACA ACTTTGAAGT CTGGACAGCC 
ACTACCCCAA CCTACTGCTA TGAGTGTGAA GGTCTGCTCT GGGGCATTGC CCGGCAGGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAAGACCT GCTCAACGCC 
GACTGCCTGC AGCGGGCTGC AGAAAAGAGT TCTAAACATG GAGCTGAGGA CCGGACCCAG 
AACATAATCA TGGCGATGAA GGACCGCATG AAGATCCGAG AGCGGAATAA GCCAGAGATC 
TTTGAGGTTA TCCGAGATGT CTTTACCGTG AGCAAAGTTG CCCATGTGCA ACAGATGAAA 
ACAGTGAAGC AGAGTGTACT GGATGGCACC TCCAAATGGT CAGCCAAAAT TACCATCACT 
GTGGTGTGTG CCCAGGGCCT ACAAGCCAAG GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TGGGCAAAAC CAAGAAGCGT ACCAAGACTA TTTTTGGGAA CCTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGACCGCAT TAAGGTACGT 
GTATGGGATG AGGATGATGA CATCAAGTCA AGAGTAAAGC AACGGCTAAA GCGAGAGTCC 
GATGATTTCC TTGGCCAAAC CATTATTGAG GTTCGGACAC TGAGTGGCGA GATGGACGTC 
TGGTACAACT TGGAGAAGAG GACAGATAAA TCAGCTGTCT CAGGGGCTAT CCGACTGCAA 
ATCAGTGTGG AGATCAAGGG AGAGGAGAAA GTAGCGCCAT ATCATGTGCA GTATACGTGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GTAGTGGAGG AGTCCTGATC 
CCTGAGGCTC GCGGAGACGA TGCATGGAAG GTGTACTTTG ATGAGACTGC CCAAGAAATT 
GTGGATGAAT TTGCCATGCG CTATGGCATC GAGTCCATAT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTGCCTG CGGTGATGAG CACCTTATTG 
GCCAACATCA ATGCCTACTA TGCCCATACA ACTGCCTCTA CCAACGTCTC TGCATCTGAT 
CGCTTTGCAG CCTCCAACTT TGGGAAAGAG AGATTTGTAA AACTACTGGA CCAGCTGCAC 
AACTCACTGA GGATTGACCT CTCTACCTAC AGGAATAATT TCCCTGCTGG AAGCCCTGAG 
CGGCTTCAGG ACTTAAAGTC AACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGAATG 
AAGGTACAAG AACTGCAGAG CCCTCCAAGA GCCAGCCAAG TGGTAAAGGA TTGTGTGAAG 
GCCTGTTTGA ACTCCACATA TGAGTATATC TTCAACAACT GCCATGACTT GTATAGTCGC 
CAGTACCAGC TGAAACAGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626765.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626822.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X2, mRNA.

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CloneID	OCe14571
Clone ID Related Accession (Same CDS sequence)	XM_005626765.2 , XM_005626765.3
Accession Version	XM_005626765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGTC ATTGGTCCAG TCCCGGAAAG CAGGAATTAC TTCTGCAATG 
GCTACACGTA CCTCTCTGAA GGATGAAGAG CTGAAATCCC ATGTGTATAA GAAAACCCTG 
CAGGCCTTAA TCTACCCAAT CTCGTGCACC ACACCCCACA ACTTTGAAGT CTGGACAGCC 
ACTACCCCAA CCTACTGCTA TGAGTGTGAA GGTCTGCTCT GGGGCATTGC CCGGCAGGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAAGACCT GCTCAACGCC 
GACTGCCTGC AGCGGGCTGC AGAAAAGAGT TCTAAACATG GAGCTGAGGA CCGGACCCAG 
AACATAATCA TGGCGATGAA GGACCGCATG AAGATCCGAG AGCGGAATAA GCCAGAGATC 
TTTGAGGTTA TCCGAGATGT CTTTACCGTG AGCAAAGTTG CCCATGTGCA ACAGATGAAA 
ACAGTGAAGC AGAGTGTACT GGATGGCACC TCCAAATGGT CAGCCAAAAT TACCATCACT 
GTGGTGTGTG CCCAGGGCCT ACAAGCCAAG GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TGGGCAAAAC CAAGAAGCGT ACCAAGACTA TTTTTGGGAA CCTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGACCGCAT TAAGGTACGT 
GTATGGGATG AGGATGATGA CATCAAGTCA AGAGTAAAGC AACGGCTAAA GCGAGAGTCC 
GATGATTTCC TTGGCCAAAC CATTATTGAG GTTCGGACAC TGAGTGGCGA GATGGACGTC 
TGGTACAACT TGGAGAAGAG GACAGATAAA TCAGCTGTCT CAGGGGCTAT CCGACTGCAA 
ATCAGTGTGG AGATCAAGGG AGAGGAGAAA GTAGCGCCAT ATCATGTGCA GTATACGTGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GTAGTGGAGG AGTCCTGATC 
CCTGAGGCTC GCGGAGACGA TGCATGGAAG GTGTACTTTG ATGAGACTGC CCAAGAAATT 
GTGGATGAAT TTGCCATGCG CTATGGCATC GAGTCCATAT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTGCCTG CGGTGATGAG CACCTTATTG 
GCCAACATCA ATGCCTACTA TGCCCATACA ACTGCCTCTA CCAACGTCTC TGCATCTGAT 
CGCTTTGCAG CCTCCAACTT TGGGAAAGAG AGATTTGTAA AACTACTGGA CCAGCTGCAC 
AACTCACTGA GGATTGACCT CTCTACCTAC AGGAATAATT TCCCTGCTGG AAGCCCTGAG 
CGGCTTCAGG ACTTAAAGTC AACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGAATG 
AAGGTACAAG AACTGCAGAG CCCTCCAAGA GCCAGCCAAG TGGTAAAGGA TTGTGTGAAG 
GCCTGTTTGA ACTCCACATA TGAGTATATC TTCAACAACT GCCATGACTT GTATAGTCGC 
CAGTACCAGC TGAAACAGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626822.1
CDS	872..6829
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626765.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626765.3

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGTC ATTGGTCCAG TCCCGGAAAG CAGGAATTAC TTCTGCAATG 
GCTACACGTA CCTCTCTGAA GGATGAAGAG CTGAAATCCC ATGTGTATAA GAAAACCCTG 
CAGGCCTTAA TCTACCCAAT CTCGTGCACC ACACCCCACA ACTTTGAAGT CTGGACAGCC 
ACTACCCCAA CCTACTGCTA TGAGTGTGAA GGTCTGCTCT GGGGCATTGC CCGGCAGGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAAGACCT GCTCAACGCC 
GACTGCCTGC AGCGGGCTGC AGAAAAGAGT TCTAAACATG GAGCTGAGGA CCGGACCCAG 
AACATAATCA TGGCGATGAA GGACCGCATG AAGATCCGAG AGCGGAATAA GCCAGAGATC 
TTTGAGGTTA TCCGAGATGT CTTTACCGTG AGCAAAGTTG CCCATGTGCA ACAGATGAAA 
ACAGTGAAGC AGAGTGTACT GGATGGCACC TCCAAATGGT CAGCCAAAAT TACCATCACT 
GTGGTGTGTG CCCAGGGCCT ACAAGCCAAG GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TGGGCAAAAC CAAGAAGCGT ACCAAGACTA TTTTTGGGAA CCTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGACCGCAT TAAGGTACGT 
GTATGGGATG AGGATGATGA CATCAAGTCA AGAGTAAAGC AACGGCTAAA GCGAGAGTCC 
GATGATTTCC TTGGCCAAAC CATTATTGAG GTTCGGACAC TGAGTGGCGA GATGGACGTC 
TGGTACAACT TGGAGAAGAG GACAGATAAA TCAGCTGTCT CAGGGGCTAT CCGACTGCAA 
ATCAGTGTGG AGATCAAGGG AGAGGAGAAA GTAGCGCCAT ATCATGTGCA GTATACGTGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GTAGTGGAGG AGTCCTGATC 
CCTGAGGCTC GCGGAGACGA TGCATGGAAG GTGTACTTTG ATGAGACTGC CCAAGAAATT 
GTGGATGAAT TTGCCATGCG CTATGGCATC GAGTCCATAT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTGCCTG CGGTGATGAG CACCTTATTG 
GCCAACATCA ATGCCTACTA TGCCCATACA ACTGCCTCTA CCAACGTCTC TGCATCTGAT 
CGCTTTGCAG CCTCCAACTT TGGGAAAGAG AGATTTGTAA AACTACTGGA CCAGCTGCAC 
AACTCACTGA GGATTGACCT CTCTACCTAC AGGAATAATT TCCCTGCTGG AAGCCCTGAG 
CGGCTTCAGG ACTTAAAGTC AACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGAATG 
AAGGTACAAG AACTGCAGAG CCCTCCAAGA GCCAGCCAAG TGGTAAAGGA TTGTGTGAAG 
GCCTGTTTGA ACTCCACATA TGAGTATATC TTCAACAACT GCCATGACTT GTATAGTCGC 
CAGTACCAGC TGAAACAGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626766.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626823.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X3, mRNA.

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CloneID	OCe14572
Clone ID Related Accession (Same CDS sequence)	XM_005626766.2 , XM_005626766.3
Accession Version	XM_005626766.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626823.1
CDS	867..6824
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626766.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626766.2

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626766.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626823.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X3, mRNA.

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CloneID	OCe14572
Clone ID Related Accession (Same CDS sequence)	XM_005626766.2 , XM_005626766.3
Accession Version	XM_005626766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626823.1
CDS	873..6830
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626766.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626766.3

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGGA GCTACCTCCA GAGGAACAAG GACCCAGCAT TCGGAACCTG 
GATTTTTGGC CCAAACTCAT CACGCTCATT GTGTCAATCA TAGAGGAAGA TAAGAATTCC 
TACACACTCG TTCTGAACCA GTTTCCTCAG GAATTGAATG TGGGAAAAGT CAGCGCAGAA 
GTGATGTGGC AGCTCTTTGC CCAAGACATG AAATATGCAC TGGAGGAGCA TGAGAAGGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATACGTGC GAGATCTGCC TGCCCTGCAG GGGCAGGTGC CTGAGTACCC AGTGTGGTTT 
GAGCAGTTTG TTCTGCAATG GCTGGATGAA AATGAGGATG TGTCCCTGGA ATTCCTGCGT 
GGGGCTCTGG AACGAGATAA GAAGGATGGG TTCCAGCAGA CATCAGAGCA TGCACTCTTT 
TCCTGCTCTG TGGTGGATGT CTTCACACAG CTCAACCAGA GCTTTGAGAT CATCCGGAAG 
CTGGAGTGCC CAGACCCCAA CATCCTTGCC CACTACATGA GGAGATTTGC TAAGACCATC 
GGGAAGGTGT TGATGCAGTA TGCAAACATC TTATCAAAGA GCTTCCCAGA TTATTGCACA 
AAGGAGAAAA TGCCCTGCAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAGGAG TTGGACCCTG AAGCTGCAGA CAGTTTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTATT TGGAAACAGT 
TTTCAGGTGC GGATTGATGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAGGTGCGA 
GGCCCAGGGA ATGCATCCCC CAACGCCAGG GCCTCTGTGG CTCAGGATGC AGATAGTGTG 
CTCCGGCCTC TCATGGACTT CCTGGATGGC AACCTCACAC TCTTTGCCAC TGTGTGTGAG 
AAGACAGTCC TGAAGCGTGT ACTGAAGGAG CTCTGGAGGG TGGTCATGAA CACAATGGAA 
AGAATGATTG TTCTGCCCCC ACTCACTGAC CAGACGGGCA CCCAGCTGAT CTTTACTGCT 
GCCAAGGAGC TGAGCCATCT TTCTAAGCTC AAGGACCACA TGGTGCGAGA GGAAACACGG 
AGTCTCACTC CAAAGCAGTG TGAAGTCCTT GACCTCGCTC TGGACACTAT CAAGCAATAC 
TTTCATGCAG GAGGTAATGG GCTGAAGAAA ACCTTCCTGG AGAAGAGCTC AGATCTGCAG 
TCTCTACGCT ATGCCCTGTC TCTCTACACA CAGACCACAG ATACACTCAT CAAAACCTTT 
GTACGCTCAC AGACTGCCCA GGTGCATGAT GGGAAAGGTA TTAGGTTTAC TGCTAATGAG 
GACATTCGGC CGGAAAAGGG ATCTGGTGTG GATGATCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGAACTGGG GAGCACAAGG TCACAGTGAA AGTGGTAGCT 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CCTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA AAAGAAGAGG AAGTTCACAA CCAAGTCAAA AAGCAACAAC 
TGGGCTCCCA AGTACAATGA GACATTTCAC TTCCTCCTGG GAAATGAAGA AGGGCCAGAA 
GCCTATGAAT TGCAAATATG TGTGAAGGAT TACTGCTTTG CCCGTGAGGA TCGTGTTTTG 
GGGCTGGCTG TGATGCCTCT GAGGGATGTA GCAGCCAAGG GCAGCTGTGC TTGCTGGTGT 
CCCTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CTATTCTCCG GATTCTATCT 
CAAAGGAGCA ATGACGAAGT GGCTCGAGAG TTTGTGAAGC TCAAATCGGA GTCTCGTTCC 
ATGGAAGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626767.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626824.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X4, mRNA.

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CloneID	OCe14573
Clone ID Related Accession (Same CDS sequence)	XM_005626767.2 , XM_005626767.3
Accession Version	XM_005626767.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGGA TCTGGTGTGG ATGATCCTGT GGGAGAAGTC 
TCTATTCAGG TGGACTTGTT TACACATCCT GGAACTGGGG AGCACAAGGT CACAGTGAAA 
GTGGTAGCTG CCAATGACCT CAAGTGGCAG ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA 
GTGACCATGG TTGGCCCACA CCAAAGTGAA AAGAAGAGGA AGTTCACAAC CAAGTCAAAA 
AGCAACAACT GGGCTCCCAA GTACAATGAG ACATTTCACT TCCTCCTGGG AAATGAAGAA 
GGGCCAGAAG CCTATGAATT GCAAATATGT GTGAAGGATT ACTGCTTTGC CCGTGAGGAT 
CGTGTTTTGG GGCTGGCTGT GATGCCTCTG AGGGATGTAG CAGCCAAGGG CAGCTGTGCT 
TGCTGGTGTC CCTTGGGCCG GAAGATCCAC ATGGATGAGA CAGGCATGAC TATTCTCCGG 
ATTCTATCTC AAAGGAGCAA TGACGAAGTG GCTCGAGAGT TTGTGAAGCT CAAATCGGAG 
TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626824.1
CDS	866..6772
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626767.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626767.2

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGGA TCTGGTGTGG ATGATCCTGT GGGAGAAGTC 
TCTATTCAGG TGGACTTGTT TACACATCCT GGAACTGGGG AGCACAAGGT CACAGTGAAA 
GTGGTAGCTG CCAATGACCT CAAGTGGCAG ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA 
GTGACCATGG TTGGCCCACA CCAAAGTGAA AAGAAGAGGA AGTTCACAAC CAAGTCAAAA 
AGCAACAACT GGGCTCCCAA GTACAATGAG ACATTTCACT TCCTCCTGGG AAATGAAGAA 
GGGCCAGAAG CCTATGAATT GCAAATATGT GTGAAGGATT ACTGCTTTGC CCGTGAGGAT 
CGTGTTTTGG GGCTGGCTGT GATGCCTCTG AGGGATGTAG CAGCCAAGGG CAGCTGTGCT 
TGCTGGTGTC CCTTGGGCCG GAAGATCCAC ATGGATGAGA CAGGCATGAC TATTCTCCGG 
ATTCTATCTC AAAGGAGCAA TGACGAAGTG GCTCGAGAGT TTGTGAAGCT CAAATCGGAG 
TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626767.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626824.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X4, mRNA.

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CloneID	OCe14573
Clone ID Related Accession (Same CDS sequence)	XM_005626767.2 , XM_005626767.3
Accession Version	XM_005626767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGGA TCTGGTGTGG ATGATCCTGT GGGAGAAGTC 
TCTATTCAGG TGGACTTGTT TACACATCCT GGAACTGGGG AGCACAAGGT CACAGTGAAA 
GTGGTAGCTG CCAATGACCT CAAGTGGCAG ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA 
GTGACCATGG TTGGCCCACA CCAAAGTGAA AAGAAGAGGA AGTTCACAAC CAAGTCAAAA 
AGCAACAACT GGGCTCCCAA GTACAATGAG ACATTTCACT TCCTCCTGGG AAATGAAGAA 
GGGCCAGAAG CCTATGAATT GCAAATATGT GTGAAGGATT ACTGCTTTGC CCGTGAGGAT 
CGTGTTTTGG GGCTGGCTGT GATGCCTCTG AGGGATGTAG CAGCCAAGGG CAGCTGTGCT 
TGCTGGTGTC CCTTGGGCCG GAAGATCCAC ATGGATGAGA CAGGCATGAC TATTCTCCGG 
ATTCTATCTC AAAGGAGCAA TGACGAAGTG GCTCGAGAGT TTGTGAAGCT CAAATCGGAG 
TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626824.1
CDS	872..6778
Translation	MKRLLQESEEEIMVTLGPPSRLSPDKAKSETMCGIKSKYSGPIESLPEDRSLLPHCGSTASAIGGDRDGGLAQHCNFEQQAGSQFPLGSTACASGSGSQDLSSVSITTSCQEPSERNQAKSLLSRGPHQGCSCEQQEPLGDVVDYIIRELQGISRLQTEIAELQQHLSQVRGSVDEVSSCVDSVLSEIEGLQVGSSSLAKVCAGEMAQEPHMDRPSEEAILYLYGLPEQDGENTMELVHSFLAMHLCVNGMQCNRYIKEAYRAGKAPAPRPTVVKLAHLEHRDFILQKSILLQSVGVRIATKEEPSWPQCYKNPQKESLSFLQQLQDDNANSLNHHEPLLQMETGDTGPITGASQNQHREPQTTEQQGLCFPPKNNFTKPSGVSKPGEEVEGTSGAFRVIGGSLGEMTGREASLSTFHQFEEPSPVLISGEEGSGKPQVLKQSSQGFEACSIAKRENNCSNKSEAGSCLSLSGLLKTEDKLLACEAGLDILSSKQLEDLLTDKSKRFATLSRDRMMEEIIIGPETFSDMVHIDLNEEEEHATQVLKDVFEKSSCGLVGLQEDEDVEIKFYKSKLGRAINHFRSALQGVFQKLENSGSISPEDLESNESGSQSENSDRLLGTMSSEGAQDFSVESPGSQESESLLSMVSGGVGISTQRDQTFQDPSNFSLASTSLPIAYSLPGFSLAPCLGSETCSRPESPKQGRLSLEQVCAETVYLNKCINNFKNVLREKRLRQKKLLHELVQKANHLSVEDIHSERKRGAIQIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626767.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626767.3

ORF Insert Sequence:

ATGAAACGAT TGTTGCAAGA GTCAGAGGAA GAAATCATGG TCACCCTGGG ACCCCCCTCC 
AGGCTTTCCC CAGATAAAGC TAAGTCAGAG ACTATGTGTG GCATCAAGAG CAAGTACTCA 
GGCCCCATTG AATCCTTACC AGAAGATAGA TCCCTGCTCC CTCACTGTGG GTCAACAGCC 
TCAGCCATTG GTGGAGATAG AGATGGGGGC CTGGCCCAGC ATTGCAACTT TGAGCAGCAG 
GCCGGCAGCC AGTTTCCTCT GGGCTCTACA GCCTGTGCTT CGGGATCTGG CTCTCAGGAT 
CTCTCATCTG TCAGCATTAC CACAAGCTGT CAAGAGCCCT CAGAAAGGAA TCAAGCCAAG 
TCCCTTTTGT CCAGAGGCCC TCACCAAGGC TGCAGCTGTG AACAGCAAGA GCCTTTGGGG 
GATGTAGTAG ACTATATAAT CAGAGAGCTA CAAGGCATCA GCCGTCTCCA AACTGAAATT 
GCTGAACTGC AGCAACACTT GAGCCAAGTC CGGGGTTCTG TGGATGAAGT GTCTAGCTGT 
GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTGCAGGTAG GCAGCAGCTC CCTGGCCAAG 
GTGTGTGCGG GGGAAATGGC CCAGGAACCC CATATGGACA GACCCAGTGA AGAAGCCATT 
CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGTACACAGC 
TTCCTGGCCA TGCACCTCTG TGTTAATGGC ATGCAATGCA ACAGGTACAT CAAAGAGGCT 
TACAGGGCAG GCAAAGCTCC AGCCCCCAGG CCTACTGTTG TGAAACTTGC CCATCTAGAG 
CATAGAGACT TCATATTGCA GAAATCCATC CTTTTGCAGA GTGTAGGAGT CCGGATTGCC 
ACCAAAGAAG AACCGAGCTG GCCACAGTGC TATAAGAATC CCCAAAAGGA GTCTCTGTCA 
TTTTTGCAAC AACTTCAGGA TGATAATGCA AATTCTTTAA ACCACCATGA GCCACTTCTT 
CAGATGGAAA CAGGGGACAC AGGACCCATA ACAGGAGCAT CTCAAAATCA ACACAGAGAA 
CCTCAAACCA CTGAGCAGCA AGGCCTTTGC TTCCCACCCA AGAATAATTT CACAAAGCCA 
AGTGGTGTAT CTAAGCCAGG AGAAGAAGTA GAAGGAACAT CAGGAGCCTT CCGAGTTATT 
GGTGGCAGTT TGGGTGAGAT GACAGGGAGA GAGGCTTCTC TGTCCACCTT CCATCAATTT 
GAGGAACCTT CCCCAGTATT AATCTCAGGA GAGGAGGGTT CTGGGAAACC CCAGGTTTTG 
AAACAGTCCA GCCAAGGATT TGAAGCATGT AGTATAGCAA AAAGAGAAAA CAACTGCAGC 
AATAAAAGTG AGGCTGGTAG CTGCCTGTCA CTGTCTGGAT TGCTGAAGAC GGAGGACAAG 
CTCCTGGCCT GTGAAGCTGG GCTTGATATT CTGAGCTCAA AGCAACTGGA GGACCTTTTG 
ACAGATAAGT CCAAAAGGTT TGCAACTCTG AGCCGTGACC GCATGATGGA GGAAATTATC 
ATAGGGCCTG AGACTTTCAG TGACATGGTT CATATTGACT TGAATGAAGA GGAGGAGCAT 
GCTACTCAGG TCCTTAAAGA TGTCTTTGAA AAGTCATCTT GTGGGCTGGT CGGTTTGCAG 
GAGGATGAAG ATGTAGAAAT TAAATTTTAC AAAAGCAAAC TGGGCCGAGC AATTAACCAT 
TTTCGTTCAG CTCTACAGGG TGTGTTTCAG AAGCTGGAGA ATAGTGGGTC CATCAGTCCT 
GAGGACCTGG AAAGCAATGA GAGTGGTTCT CAGTCAGAGA ACAGTGATAG ACTTCTTGGT 
ACAATGAGTT CAGAAGGTGC GCAAGATTTC TCTGTGGAGA GCCCTGGGAG TCAGGAGAGT 
GAGAGCTTGC TCAGTATGGT ATCTGGTGGA GTGGGCATTT CTACACAGAG AGACCAAACC 
TTTCAGGATC CTAGCAACTT CTCTCTGGCT TCTACCTCCT TGCCTATCGC ATATTCATTG 
CCAGGTTTTT CTCTGGCTCC ATGTCTGGGA AGTGAAACTT GTTCCAGGCC TGAATCTCCC 
AAGCAGGGCA GACTAAGCCT AGAGCAAGTG TGTGCAGAGA CTGTCTACCT CAACAAGTGC 
ATCAACAATT TTAAAAATGT TCTGAGAGAG AAAAGACTGA GGCAGAAAAA ACTTTTGCAT 
GAGCTAGTAC AGAAGGCAAA CCATCTCTCA GTAGAAGACA TACACTCAGA AAGAAAGCGA 
GGAGCCATTC AGATTCCGGA TGATGGTCAC CCTTCTTTGC CCCAGTGGCT TCCGGAAGGG 
CCTGCTGGAG GGCTCTATGG CATTGACAGC ATGCCAGATC TACGCAGAAA GAAACCATTG 
CCACTTGTCA GTGATCTGGC TATGTCATTG GTCCAGTCCC GGAAAGCAGG AATTACTTCT 
GCAATGGCTA CACGTACCTC TCTGAAGGAT GAAGAGCTGA AATCCCATGT GTATAAGAAA 
ACCCTGCAGG CCTTAATCTA CCCAATCTCG TGCACCACAC CCCACAACTT TGAAGTCTGG 
ACAGCCACTA CCCCAACCTA CTGCTATGAG TGTGAAGGTC TGCTCTGGGG CATTGCCCGG 
CAGGGCATGC GCTGCAGCGA GTGTGGAGTC AAGTGCCATG AGAAGTGCCA AGACCTGCTC 
AACGCCGACT GCCTGCAGCG GGCTGCAGAA AAGAGTTCTA AACATGGAGC TGAGGACCGG 
ACCCAGAACA TAATCATGGC GATGAAGGAC CGCATGAAGA TCCGAGAGCG GAATAAGCCA 
GAGATCTTTG AGGTTATCCG AGATGTCTTT ACCGTGAGCA AAGTTGCCCA TGTGCAACAG 
ATGAAAACAG TGAAGCAGAG TGTACTGGAT GGCACCTCCA AATGGTCAGC CAAAATTACC 
ATCACTGTGG TGTGTGCCCA GGGCCTACAA GCCAAGGACA AGACAGGATC CAGTGACCCT 
TATGTGACTG TGCAAGTGGG CAAAACCAAG AAGCGTACCA AGACTATTTT TGGGAACCTG 
AATCCTGTTT GGGAAGAGAA GTTCCATTTT GAGTGCCACA ACTCTTCTGA CCGCATTAAG 
GTACGTGTAT GGGATGAGGA TGATGACATC AAGTCAAGAG TAAAGCAACG GCTAAAGCGA 
GAGTCCGATG ATTTCCTTGG CCAAACCATT ATTGAGGTTC GGACACTGAG TGGCGAGATG 
GACGTCTGGT ACAACTTGGA GAAGAGGACA GATAAATCAG CTGTCTCAGG GGCTATCCGA 
CTGCAAATCA GTGTGGAGAT CAAGGGAGAG GAGAAAGTAG CGCCATATCA TGTGCAGTAT 
ACGTGTCTCC ATGAGAACCT TTTCCATTAC CTCACAGACA TTCAGGGTAG TGGAGGAGTC 
CTGATCCCTG AGGCTCGCGG AGACGATGCA TGGAAGGTGT ACTTTGATGA GACTGCCCAA 
GAAATTGTGG ATGAATTTGC CATGCGCTAT GGCATCGAGT CCATATATCA GGCCATGACG 
CACTTTGCAT GTTTGTCATC CAAGTACATG TGTCCTGGTG TGCCTGCGGT GATGAGCACC 
TTATTGGCCA ACATCAATGC CTACTATGCC CATACAACTG CCTCTACCAA CGTCTCTGCA 
TCTGATCGCT TTGCAGCCTC CAACTTTGGG AAAGAGAGAT TTGTAAAACT ACTGGACCAG 
CTGCACAACT CACTGAGGAT TGACCTCTCT ACCTACAGGA ATAATTTCCC TGCTGGAAGC 
CCTGAGCGGC TTCAGGACTT AAAGTCAACA GTGGATTTGC TGACCAGCAT TACTTTCTTC 
AGAATGAAGG TACAAGAACT GCAGAGCCCT CCAAGAGCCA GCCAAGTGGT AAAGGATTGT 
GTGAAGGCCT GTTTGAACTC CACATATGAG TATATCTTCA ACAACTGCCA TGACTTGTAT 
AGTCGCCAGT ACCAGCTGAA ACAGGAGCTA CCTCCAGAGG AACAAGGACC CAGCATTCGG 
AACCTGGATT TTTGGCCCAA ACTCATCACG CTCATTGTGT CAATCATAGA GGAAGATAAG 
AATTCCTACA CACTCGTTCT GAACCAGTTT CCTCAGGAAT TGAATGTGGG AAAAGTCAGC 
GCAGAAGTGA TGTGGCAGCT CTTTGCCCAA GACATGAAAT ATGCACTGGA GGAGCATGAG 
AAGGACCGGC TGTGTAAGAG TGCTGACTAC ATGAACCTGC ACTTCAAGGT GAAGTGGCTC 
CACAATGAAT ACGTGCGAGA TCTGCCTGCC CTGCAGGGGC AGGTGCCTGA GTACCCAGTG 
TGGTTTGAGC AGTTTGTTCT GCAATGGCTG GATGAAAATG AGGATGTGTC CCTGGAATTC 
CTGCGTGGGG CTCTGGAACG AGATAAGAAG GATGGGTTCC AGCAGACATC AGAGCATGCA 
CTCTTTTCCT GCTCTGTGGT GGATGTCTTC ACACAGCTCA ACCAGAGCTT TGAGATCATC 
CGGAAGCTGG AGTGCCCAGA CCCCAACATC CTTGCCCACT ACATGAGGAG ATTTGCTAAG 
ACCATCGGGA AGGTGTTGAT GCAGTATGCA AACATCTTAT CAAAGAGCTT CCCAGATTAT 
TGCACAAAGG AGAAAATGCC CTGCATCCTG ATGAACAACA TGCAGCAACT GAGGGTCCAG 
CTGGAGAAAA TGTTTGAGGC CATGGGAGGC AAGGAGTTGG ACCCTGAAGC TGCAGACAGT 
TTGAAGGAGC TGCAGGTGAA ACTGAATACA GTTCTGGATG AGCTCAGCAT GGTATTTGGA 
AACAGTTTTC AGGTGCGGAT TGATGAGTGT GTTCGACAAA TGGCTGACAT CCTGGGCCAG 
GTGCGAGGCC CAGGGAATGC ATCCCCCAAC GCCAGGGCCT CTGTGGCTCA GGATGCAGAT 
AGTGTGCTCC GGCCTCTCAT GGACTTCCTG GATGGCAACC TCACACTCTT TGCCACTGTG 
TGTGAGAAGA CAGTCCTGAA GCGTGTACTG AAGGAGCTCT GGAGGGTGGT CATGAACACA 
ATGGAAAGAA TGATTGTTCT GCCCCCACTC ACTGACCAGA CGGGCACCCA GCTGATCTTT 
ACTGCTGCCA AGGAGCTGAG CCATCTTTCT AAGCTCAAGG ACCACATGGT GCGAGAGGAA 
ACACGGAGTC TCACTCCAAA GCAGTGTGAA GTCCTTGACC TCGCTCTGGA CACTATCAAG 
CAATACTTTC ATGCAGGAGG TAATGGGCTG AAGAAAACCT TCCTGGAGAA GAGCTCAGAT 
CTGCAGTCTC TACGCTATGC CCTGTCTCTC TACACACAGA CCACAGATAC ACTCATCAAA 
ACCTTTGTAC GCTCACAGAC TGCCCAGGGA TCTGGTGTGG ATGATCCTGT GGGAGAAGTC 
TCTATTCAGG TGGACTTGTT TACACATCCT GGAACTGGGG AGCACAAGGT CACAGTGAAA 
GTGGTAGCTG CCAATGACCT CAAGTGGCAG ACAGCGGGTA TGTTCCGGCC CTTTGTGGAA 
GTGACCATGG TTGGCCCACA CCAAAGTGAA AAGAAGAGGA AGTTCACAAC CAAGTCAAAA 
AGCAACAACT GGGCTCCCAA GTACAATGAG ACATTTCACT TCCTCCTGGG AAATGAAGAA 
GGGCCAGAAG CCTATGAATT GCAAATATGT GTGAAGGATT ACTGCTTTGC CCGTGAGGAT 
CGTGTTTTGG GGCTGGCTGT GATGCCTCTG AGGGATGTAG CAGCCAAGGG CAGCTGTGCT 
TGCTGGTGTC CCTTGGGCCG GAAGATCCAC ATGGATGAGA CAGGCATGAC TATTCTCCGG 
ATTCTATCTC AAAGGAGCAA TGACGAAGTG GCTCGAGAGT TTGTGAAGCT CAAATCGGAG 
TCTCGTTCCA TGGAAGAGGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626768.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626825.1 Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X12, mRNA.

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CloneID	OCe14574
Clone ID Related Accession (Same CDS sequence)	XM_005626768.2 , XM_005626768.3
Accession Version	XM_005626768.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein unc-13 homolog B isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCAGCA GACTTTTGAA GATCCTGATA GTGCTGTTGA TGACCGGGAT 
AGTGACTATC GCAGTGAGAC CAGCAATAGC ATCCCACCTC CTTATCATAC AACTTCCCAG 
CCCAACGCTT CTGTGCACCA GTTTCCTGTG CCCATGCGAT TGCCACAGCA GCTGCTACTT 
CAGGGCAATT CCCGGGATTC TTGCAATGAC TCCATGCAAA GTTACGATCT TGATTATCCA 
GAGCGGCGGG CCCTTAGCCC CACCAGCAGT AGTAGGTATG GCTCATCCTG TAATGTGAGT 
CAAGGAAGCT CTCAGTTGAG TGAACTAGAC CAGTGTCACG AACAAGATGA TGACCGTCGG 
GAGAGGGATT CAATTCATTC ATGCCACAGC TCTGGCAGCT TCTCCAGAGA CGGCCAGGTG 
GGCTTTGGAG AACAAGAGAA AGCCTTGGAG GTAACAGGTG AAGAAGAGAA GGGGAGAGTG 
TGTGAACCCA AGGACAAGAA AAAAGATACC ACAACCCATC TTCCCCCAGA TCTGGTGCTA 
CCCAAAGACC ACGTCCTAGG CCCCCAGGAG AGTTTTCCTG AGGAGAATGG TTCTTCACCA 
TTTACCCAAG CAAGAGCTCA CTGGATCCGA GCAGTTACCA AGGTTCGACT CCAGCTGCAG 
GAGATTCCGG ATGATGGTCA CCCTTCTTTG CCCCAGTGGC TTCCGGAAGG GCCTGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGCAGAA AGAAACCATT GCCACTTGTC 
AGTGATCTGG CTATGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGT GCATGATGGG AAAGGTATTA GGTTTACTGC TAATGAGGAC 
ATTCGGCCGG AAAAGGGATC TGGTGTGGAT GATCCTGTGG GAGAAGTCTC TATTCAGGTG 
GACTTGTTTA CACATCCTGG AACTGGGGAG CACAAGGTCA CAGTGAAAGT GGTAGCTGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCCT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGAAAA GAAGAGGAAG TTCACAACCA AGTCAAAAAG CAACAACTGG 
GCTCCCAAGT ACAATGAGAC ATTTCACTTC CTCCTGGGAA ATGAAGAAGG GCCAGAAGCC 
TATGAATTGC AAATATGTGT GAAGGATTAC TGCTTTGCCC GTGAGGATCG TGTTTTGGGG 
CTGGCTGTGA TGCCTCTGAG GGATGTAGCA GCCAAGGGCA GCTGTGCTTG CTGGTGTCCC 
TTGGGCCGGA AGATCCACAT GGATGAGACA GGCATGACTA TTCTCCGGAT TCTATCTCAA 
AGGAGCAATG ACGAAGTGGC TCGAGAGTTT GTGAAGCTCA AATCGGAGTC TCGTTCCATG 
GAAGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626825.1
CDS	362..5236
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCCHWTYLGWGEQQTFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626768.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris unc-13 homolog B (C. elegans) (UNC13B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626768.2

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCAGCA GACTTTTGAA GATCCTGATA GTGCTGTTGA TGACCGGGAT 
AGTGACTATC GCAGTGAGAC CAGCAATAGC ATCCCACCTC CTTATCATAC AACTTCCCAG 
CCCAACGCTT CTGTGCACCA GTTTCCTGTG CCCATGCGAT TGCCACAGCA GCTGCTACTT 
CAGGGCAATT CCCGGGATTC TTGCAATGAC TCCATGCAAA GTTACGATCT TGATTATCCA 
GAGCGGCGGG CCCTTAGCCC CACCAGCAGT AGTAGGTATG GCTCATCCTG TAATGTGAGT 
CAAGGAAGCT CTCAGTTGAG TGAACTAGAC CAGTGTCACG AACAAGATGA TGACCGTCGG 
GAGAGGGATT CAATTCATTC ATGCCACAGC TCTGGCAGCT TCTCCAGAGA CGGCCAGGTG 
GGCTTTGGAG AACAAGAGAA AGCCTTGGAG GTAACAGGTG AAGAAGAGAA GGGGAGAGTG 
TGTGAACCCA AGGACAAGAA AAAAGATACC ACAACCCATC TTCCCCCAGA TCTGGTGCTA 
CCCAAAGACC ACGTCCTAGG CCCCCAGGAG AGTTTTCCTG AGGAGAATGG TTCTTCACCA 
TTTACCCAAG CAAGAGCTCA CTGGATCCGA GCAGTTACCA AGGTTCGACT CCAGCTGCAG 
GAGATTCCGG ATGATGGTCA CCCTTCTTTG CCCCAGTGGC TTCCGGAAGG GCCTGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGCAGAA AGAAACCATT GCCACTTGTC 
AGTGATCTGG CTATGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGT GCATGATGGG AAAGGTATTA GGTTTACTGC TAATGAGGAC 
ATTCGGCCGG AAAAGGGATC TGGTGTGGAT GATCCTGTGG GAGAAGTCTC TATTCAGGTG 
GACTTGTTTA CACATCCTGG AACTGGGGAG CACAAGGTCA CAGTGAAAGT GGTAGCTGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCCT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGAAAA GAAGAGGAAG TTCACAACCA AGTCAAAAAG CAACAACTGG 
GCTCCCAAGT ACAATGAGAC ATTTCACTTC CTCCTGGGAA ATGAAGAAGG GCCAGAAGCC 
TATGAATTGC AAATATGTGT GAAGGATTAC TGCTTTGCCC GTGAGGATCG TGTTTTGGGG 
CTGGCTGTGA TGCCTCTGAG GGATGTAGCA GCCAAGGGCA GCTGTGCTTG CTGGTGTCCC 
TTGGGCCGGA AGATCCACAT GGATGAGACA GGCATGACTA TTCTCCGGAT TCTATCTCAA 
AGGAGCAATG ACGAAGTGGC TCGAGAGTTT GTGAAGCTCA AATCGGAGTC TCGTTCCATG 
GAAGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13B ( XM_005626768.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626825.1 Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X5, mRNA.

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CloneID	OCe14574
Clone ID Related Accession (Same CDS sequence)	XM_005626768.2 , XM_005626768.3
Accession Version	XM_005626768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein unc-13 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCAGCA GACTTTTGAA GATCCTGATA GTGCTGTTGA TGACCGGGAT 
AGTGACTATC GCAGTGAGAC CAGCAATAGC ATCCCACCTC CTTATCATAC AACTTCCCAG 
CCCAACGCTT CTGTGCACCA GTTTCCTGTG CCCATGCGAT TGCCACAGCA GCTGCTACTT 
CAGGGCAATT CCCGGGATTC TTGCAATGAC TCCATGCAAA GTTACGATCT TGATTATCCA 
GAGCGGCGGG CCCTTAGCCC CACCAGCAGT AGTAGGTATG GCTCATCCTG TAATGTGAGT 
CAAGGAAGCT CTCAGTTGAG TGAACTAGAC CAGTGTCACG AACAAGATGA TGACCGTCGG 
GAGAGGGATT CAATTCATTC ATGCCACAGC TCTGGCAGCT TCTCCAGAGA CGGCCAGGTG 
GGCTTTGGAG AACAAGAGAA AGCCTTGGAG GTAACAGGTG AAGAAGAGAA GGGGAGAGTG 
TGTGAACCCA AGGACAAGAA AAAAGATACC ACAACCCATC TTCCCCCAGA TCTGGTGCTA 
CCCAAAGACC ACGTCCTAGG CCCCCAGGAG AGTTTTCCTG AGGAGAATGG TTCTTCACCA 
TTTACCCAAG CAAGAGCTCA CTGGATCCGA GCAGTTACCA AGGTTCGACT CCAGCTGCAG 
GAGATTCCGG ATGATGGTCA CCCTTCTTTG CCCCAGTGGC TTCCGGAAGG GCCTGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGCAGAA AGAAACCATT GCCACTTGTC 
AGTGATCTGG CTATGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGT GCATGATGGG AAAGGTATTA GGTTTACTGC TAATGAGGAC 
ATTCGGCCGG AAAAGGGATC TGGTGTGGAT GATCCTGTGG GAGAAGTCTC TATTCAGGTG 
GACTTGTTTA CACATCCTGG AACTGGGGAG CACAAGGTCA CAGTGAAAGT GGTAGCTGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCCT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGAAAA GAAGAGGAAG TTCACAACCA AGTCAAAAAG CAACAACTGG 
GCTCCCAAGT ACAATGAGAC ATTTCACTTC CTCCTGGGAA ATGAAGAAGG GCCAGAAGCC 
TATGAATTGC AAATATGTGT GAAGGATTAC TGCTTTGCCC GTGAGGATCG TGTTTTGGGG 
CTGGCTGTGA TGCCTCTGAG GGATGTAGCA GCCAAGGGCA GCTGTGCTTG CTGGTGTCCC 
TTGGGCCGGA AGATCCACAT GGATGAGACA GGCATGACTA TTCTCCGGAT TCTATCTCAA 
AGGAGCAATG ACGAAGTGGC TCGAGAGTTT GTGAAGCTCA AATCGGAGTC TCGTTCCATG 
GAAGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626825.1
CDS	335..5209
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTVRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINVLETDSEYSIQEESQRKPLPTAAAQCCHWTYLGWGEQQTFEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASVHQFPVPMRLPQQLLLQGNSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSQLSELDQCHEQDDDRRERDSIHSCHSSGSFSRDGQVGFGEQEKALEVTGEEEKGRVCEPKDKKKDTTTHLPPDLVLPKDHVLGPQESFPEENGSSPFTQARAHWIRAVTKVRLQLQEIPDDGHPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELSHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSMEEGS

Target ORF information:

RefSeq Version	XM_005626768.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris unc-13 homolog B (UNC13B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626768.3

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTACG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTGCAGAAT GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAACCTT CTTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTA 
AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTTGGGAC TGTGTGGATT 
GCACTGAAGA CTGTTCGTCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC CACGTTGGAG 
GCAGAGACAT TGATGAAAGA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAG 
ATTTTGCTTG ATACAAGATT TGAGCTGCCT TTTGATATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATCAATGTC TTGGAAACTG ACAGTGAGTA TTCTATTCAA 
GAAGAAAGCC AGAGGAAGCC ATTGCCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCAGCA GACTTTTGAA GATCCTGATA GTGCTGTTGA TGACCGGGAT 
AGTGACTATC GCAGTGAGAC CAGCAATAGC ATCCCACCTC CTTATCATAC AACTTCCCAG 
CCCAACGCTT CTGTGCACCA GTTTCCTGTG CCCATGCGAT TGCCACAGCA GCTGCTACTT 
CAGGGCAATT CCCGGGATTC TTGCAATGAC TCCATGCAAA GTTACGATCT TGATTATCCA 
GAGCGGCGGG CCCTTAGCCC CACCAGCAGT AGTAGGTATG GCTCATCCTG TAATGTGAGT 
CAAGGAAGCT CTCAGTTGAG TGAACTAGAC CAGTGTCACG AACAAGATGA TGACCGTCGG 
GAGAGGGATT CAATTCATTC ATGCCACAGC TCTGGCAGCT TCTCCAGAGA CGGCCAGGTG 
GGCTTTGGAG AACAAGAGAA AGCCTTGGAG GTAACAGGTG AAGAAGAGAA GGGGAGAGTG 
TGTGAACCCA AGGACAAGAA AAAAGATACC ACAACCCATC TTCCCCCAGA TCTGGTGCTA 
CCCAAAGACC ACGTCCTAGG CCCCCAGGAG AGTTTTCCTG AGGAGAATGG TTCTTCACCA 
TTTACCCAAG CAAGAGCTCA CTGGATCCGA GCAGTTACCA AGGTTCGACT CCAGCTGCAG 
GAGATTCCGG ATGATGGTCA CCCTTCTTTG CCCCAGTGGC TTCCGGAAGG GCCTGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGCAGAA AGAAACCATT GCCACTTGTC 
AGTGATCTGG CTATGTCATT GGTCCAGTCC CGGAAAGCAG GAATTACTTC TGCAATGGCT 
ACACGTACCT CTCTGAAGGA TGAAGAGCTG AAATCCCATG TGTATAAGAA AACCCTGCAG 
GCCTTAATCT ACCCAATCTC GTGCACCACA CCCCACAACT TTGAAGTCTG GACAGCCACT 
ACCCCAACCT ACTGCTATGA GTGTGAAGGT CTGCTCTGGG GCATTGCCCG GCAGGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AAGACCTGCT CAACGCCGAC 
TGCCTGCAGC GGGCTGCAGA AAAGAGTTCT AAACATGGAG CTGAGGACCG GACCCAGAAC 
ATAATCATGG CGATGAAGGA CCGCATGAAG ATCCGAGAGC GGAATAAGCC AGAGATCTTT 
GAGGTTATCC GAGATGTCTT TACCGTGAGC AAAGTTGCCC ATGTGCAACA GATGAAAACA 
GTGAAGCAGA GTGTACTGGA TGGCACCTCC AAATGGTCAG CCAAAATTAC CATCACTGTG 
GTGTGTGCCC AGGGCCTACA AGCCAAGGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTGG GCAAAACCAA GAAGCGTACC AAGACTATTT TTGGGAACCT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ACCGCATTAA GGTACGTGTA 
TGGGATGAGG ATGATGACAT CAAGTCAAGA GTAAAGCAAC GGCTAAAGCG AGAGTCCGAT 
GATTTCCTTG GCCAAACCAT TATTGAGGTT CGGACACTGA GTGGCGAGAT GGACGTCTGG 
TACAACTTGG AGAAGAGGAC AGATAAATCA GCTGTCTCAG GGGCTATCCG ACTGCAAATC 
AGTGTGGAGA TCAAGGGAGA GGAGAAAGTA GCGCCATATC ATGTGCAGTA TACGTGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGTA GTGGAGGAGT CCTGATCCCT 
GAGGCTCGCG GAGACGATGC ATGGAAGGTG TACTTTGATG AGACTGCCCA AGAAATTGTG 
GATGAATTTG CCATGCGCTA TGGCATCGAG TCCATATATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTGCCTGCGG TGATGAGCAC CTTATTGGCC 
AACATCAATG CCTACTATGC CCATACAACT GCCTCTACCA ACGTCTCTGC ATCTGATCGC 
TTTGCAGCCT CCAACTTTGG GAAAGAGAGA TTTGTAAAAC TACTGGACCA GCTGCACAAC 
TCACTGAGGA TTGACCTCTC TACCTACAGG AATAATTTCC CTGCTGGAAG CCCTGAGCGG 
CTTCAGGACT TAAAGTCAAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGAATGAAG 
GTACAAGAAC TGCAGAGCCC TCCAAGAGCC AGCCAAGTGG TAAAGGATTG TGTGAAGGCC 
TGTTTGAACT CCACATATGA GTATATCTTC AACAACTGCC ATGACTTGTA TAGTCGCCAG 
TACCAGCTGA AACAGGAGCT ACCTCCAGAG GAACAAGGAC CCAGCATTCG GAACCTGGAT 
TTTTGGCCCA AACTCATCAC GCTCATTGTG TCAATCATAG AGGAAGATAA GAATTCCTAC 
ACACTCGTTC TGAACCAGTT TCCTCAGGAA TTGAATGTGG GAAAAGTCAG CGCAGAAGTG 
ATGTGGCAGC TCTTTGCCCA AGACATGAAA TATGCACTGG AGGAGCATGA GAAGGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TACGTGCGAG ATCTGCCTGC CCTGCAGGGG CAGGTGCCTG AGTACCCAGT GTGGTTTGAG 
CAGTTTGTTC TGCAATGGCT GGATGAAAAT GAGGATGTGT CCCTGGAATT CCTGCGTGGG 
GCTCTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC ACTCTTTTCC 
TGCTCTGTGG TGGATGTCTT CACACAGCTC AACCAGAGCT TTGAGATCAT CCGGAAGCTG 
GAGTGCCCAG ACCCCAACAT CCTTGCCCAC TACATGAGGA GATTTGCTAA GACCATCGGG 
AAGGTGTTGA TGCAGTATGC AAACATCTTA TCAAAGAGCT TCCCAGATTA TTGCACAAAG 
GAGAAAATGC CCTGCATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAGGAGTTG GACCCTGAAG CTGCAGACAG TTTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTCAGCA TGGTATTTGG AAACAGTTTT 
CAGGTGCGGA TTGATGAGTG TGTTCGACAA ATGGCTGACA TCCTGGGCCA GGTGCGAGGC 
CCAGGGAATG CATCCCCCAA CGCCAGGGCC TCTGTGGCTC AGGATGCAGA TAGTGTGCTC 
CGGCCTCTCA TGGACTTCCT GGATGGCAAC CTCACACTCT TTGCCACTGT GTGTGAGAAG 
ACAGTCCTGA AGCGTGTACT GAAGGAGCTC TGGAGGGTGG TCATGAACAC AATGGAAAGA 
ATGATTGTTC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT TACTGCTGCC 
AAGGAGCTGA GCCATCTTTC TAAGCTCAAG GACCACATGG TGCGAGAGGA AACACGGAGT 
CTCACTCCAA AGCAGTGTGA AGTCCTTGAC CTCGCTCTGG ACACTATCAA GCAATACTTT 
CATGCAGGAG GTAATGGGCT GAAGAAAACC TTCCTGGAGA AGAGCTCAGA TCTGCAGTCT 
CTACGCTATG CCCTGTCTCT CTACACACAG ACCACAGATA CACTCATCAA AACCTTTGTA 
CGCTCACAGA CTGCCCAGGT GCATGATGGG AAAGGTATTA GGTTTACTGC TAATGAGGAC 
ATTCGGCCGG AAAAGGGATC TGGTGTGGAT GATCCTGTGG GAGAAGTCTC TATTCAGGTG 
GACTTGTTTA CACATCCTGG AACTGGGGAG CACAAGGTCA CAGTGAAAGT GGTAGCTGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCCT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGAAAA GAAGAGGAAG TTCACAACCA AGTCAAAAAG CAACAACTGG 
GCTCCCAAGT ACAATGAGAC ATTTCACTTC CTCCTGGGAA ATGAAGAAGG GCCAGAAGCC 
TATGAATTGC AAATATGTGT GAAGGATTAC TGCTTTGCCC GTGAGGATCG TGTTTTGGGG 
CTGGCTGTGA TGCCTCTGAG GGATGTAGCA GCCAAGGGCA GCTGTGCTTG CTGGTGTCCC 
TTGGGCCGGA AGATCCACAT GGATGAGACA GGCATGACTA TTCTCCGGAT TCTATCTCAA 
AGGAGCAATG ACGAAGTGGC TCGAGAGTTT GTGAAGCTCA AATCGGAGTC TCGTTCCATG 
GAAGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	UNC13B
Entrez Gene ID	100683511
Full Name	unc-13 homolog B
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Canis lupus familiaris (dog)	UNC13B	XP_005626825.1
Mus musculus (house mouse)	Unc13b	NP_067443.2
Gallus gallus (chicken)	LOC100858799	XP_004937184.1
Pan troglodytes (chimpanzee)	UNC13B	XP_519737.2
	UNC13B	XP_001085980.2
Danio rerio (zebrafish)	LOC100329499	XP_002663560.3
Homo sapiens (human)	UNC13B	NP_006368.3
Bos taurus (cattle)	UNC13B	NP_001180168.1
Rattus norvegicus (Norway rat)	Unc13b	NP_001036044.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

UNC13B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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