LOC100683419 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014120602.1	XP_013976077.1	myomegalin isoform X17
XM_014120599.1	XP_013976074.1	myomegalin isoform X12
XM_005630692.2	XP_005630749.1	myomegalin isoform X13
XM_005630689.3	XP_005630746.1	myomegalin isoform X2
XM_014120597.2	XP_013976072.1	myomegalin isoform X10
XM_005630692.3	XP_005630749.1	myomegalin isoform X14
XM_014120592.1	XP_013976067.1	myomegalin isoform X3
XM_014120595.1	XP_013976070.1	myomegalin isoform X8
XM_005630690.2	XP_005630747.1	myomegalin isoform X4
XM_005630690.3	XP_005630747.1	myomegalin isoform X4
XM_014120594.2	XP_013976069.1	myomegalin isoform X6
XM_014120595.2	XP_013976070.1	myomegalin isoform X8
XM_022404897.1	XP_022260605.1	myomegalin isoform X11
XM_014120594.1	XP_013976069.1	myomegalin isoform X6
XM_005630689.2	XP_005630746.1	myomegalin isoform X2
XM_005630691.2	XP_005630748.1	myomegalin isoform X7
XM_003432239.3	XP_003432287.1	myomegalin isoform X15
XM_014120601.1	XP_013976076.1	myomegalin isoform X16
XM_014120592.2	XP_013976067.1	myomegalin isoform X3
XM_003432239.4	XP_003432287.1	myomegalin isoform X15
XM_014120601.2	XP_013976076.1	myomegalin isoform X17
XM_022404899.1	XP_022260607.1	myomegalin isoform X18
XM_014120593.1	XP_013976068.1	myomegalin isoform X5
XM_014120596.1	XP_013976071.1	myomegalin isoform X9
XM_014120598.1	XP_013976073.1	myomegalin isoform X11
XM_005630691.3	XP_005630748.1	myomegalin isoform X7
XM_014120596.2	XP_013976071.1	myomegalin isoform X9
XM_014120598.2	XP_013976073.1	myomegalin isoform X12
XM_022404898.1	XP_022260606.1	myomegalin isoform X16
XM_014120602.2	XP_013976077.1	myomegalin isoform X19
XM_014120591.1	XP_013976066.1	myomegalin isoform X1
XM_014120597.1	XP_013976072.1	myomegalin isoform X10
XM_014120600.1	XP_013976075.1	myomegalin isoform X14
XM_014120591.2	XP_013976066.1	myomegalin isoform X1
XM_014120593.2	XP_013976068.1	myomegalin isoform X5
XM_014120599.2	XP_013976074.1	myomegalin isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe19363	XM_003432239.4 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe294492	XM_022404898.1 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe40885	XM_014120599.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe40886	XM_014120600.1 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe40887	XM_014120601.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe19357	XM_005630690.3 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe19359	XM_005630692.3 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe19356	XM_005630689.3 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe19358	XM_005630691.3 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe294491	XM_022404897.1 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe294493	XM_022404899.1 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40877	XM_014120591.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40878	XM_014120592.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40879	XM_014120593.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40880	XM_014120594.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40881	XM_014120595.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40882	XM_014120596.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40883	XM_014120597.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40884	XM_014120598.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40888	XM_014120602.2 Latest version!	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
View Old Accession Versions >>
OCe19359	XM_005630692.2	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe19357	XM_005630690.2	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe40881	XM_014120595.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40880	XM_014120594.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe19356	XM_005630689.2	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40878	XM_014120592.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe19363	XM_003432239.3	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe40887	XM_014120601.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe19358	XM_005630691.2	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40882	XM_014120596.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40884	XM_014120598.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40888	XM_014120602.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40883	XM_014120597.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40877	XM_014120591.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40879	XM_014120593.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe40885	XM_014120599.1	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

LOC100683419 ( XM_003432239.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432287.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe19363
Clone ID Related Accession (Same CDS sequence)	XM_003432239.3 , XM_003432239.4
Accession Version	XM_003432239.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432239.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432287.1
CDS	28..558
Translation	MFLCQGFRKYLPEHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDK

Target ORF information:

RefSeq Version	XM_003432239.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432239.3

ORF Insert Sequence:

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_003432239.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432287.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe19363
Clone ID Related Accession (Same CDS sequence)	XM_003432239.3 , XM_003432239.4
Accession Version	XM_003432239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432239.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432287.1
CDS	31..561
Translation	MFLCQGFRKYLPEHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDK

Target ORF information:

RefSeq Version	XM_003432239.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432239.4

ORF Insert Sequence:

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_022404898.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260606.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe294492
Clone ID Related Accession (Same CDS sequence)	XM_022404898.1
Accession Version	XM_022404898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA GCTGCAGGAT CTGTGCCCGG GAGCTGTGTG GGAACCAGCG GCGCTGGATC 
TTCCACACGG CATCTAAACT CAACCTCCAG GTTCTGCTCT CGCACGTCCT GGGCAAGGAT 
GTGCCCCGCG ATGGCAAAGC CGAGTTTGCT TGCAGCAAAT GTGCTTTCAT GCTTGATCGG 
ATCTATAGAT TCGACACCGT TATTGCACGG ATCGAAGCTC TTTCTATCGA GCGTTTGCAA 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGAGGACT CTGGCACGGA AACCAAGGCG GGGAATGGGA CCGTTGACAT TTCCGGCTTA 
CCCGACGTAA GATACTCTGC GCTGCTCCAG GAGGACTTTG CCTATTCAGG GTTTGAGTGT 
TGGGTAGAAA ACGAGGATCA GATTCAGGAG CTACACAGCT GCCATACTTC GGAAGGTCCT 
GGAAACCGAC CCAGGAGATG CCGTGGTTGT GCAGCCTTGC GGGTTGCTGA TTCTGACTAT 
GAAGCCATTT GTAAGGTGCC CCGAAAGGTG GCCAGAAGTA TCTCGTGTGC TCCTTCTAGG 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCATTGTCTG AGGTTGGGCC ACCGGATTTA 
CCAAGTACAA AGGTACCCCC AGATGGAGAG AGCATGGAGG AAGGGACACC CGGATCCTCT 
GTGGAATCTT TGGATGCAAG TGTCCAGGCT AGTCCTCCAC AACAAAAGGA TGAGGAAACG 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGTTGTT CAGATGAACA GGCTCCACAG 
CATGTGTGTA ACCACAAGCT AGAGCTAGCT CTTAACATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GTCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAG CCCACCTGGA 
GCCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTCTCCTCAA CAGGTCTTTG 
AAACCCCTTT ACAAGATACC TGTGAATTAT CCCTTGGAGC TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TTTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCTGTGAC TGAAGAGTTA 
CTCAAGCAAC AAAAGCTGGA TTCAGATGAG ACCATTATAA CTCAGCAGTC TGTATCTGAT 
TCCCACTTGG CTGAACTCCA GGAAAAAATC CAGCAAATGG AGGCCACCAA CAAAATTCTT 
CAAGAGAAAC TGAATGAAAT GAGCTGTGAG CTAAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGGTA CAATTCAAAG CCTCAAGGAA ACTCTGAAAA GTAGGGAAAA TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAT GACACAATGG CAAAGCTTCG AGAAATGCTA 
CACCAAAGCC AGCTTGGACA ACTTCATGTA TTTGAGGGTC ACATAGGACA GGAGAGCTCA 
CAGGGTACTT CCCCAGCTCA GCAACAGATG GCTCTGCTTG ATCTTCAGAG TGGTTTGTTC 
TACAGCCAGC TTGAGATTCA GAAACTCCAG AGGTTGGTAC GTCAAAAAGA ACGCCAACTG 
GCTGATGCCA AACGATGTGT CCAATTTGTA GAGGCTGCAG CCCATGAAAG AGAACAGCAG 
AAAGAGGCAT CTTGGAAACA TAATCAGGAA TTACGGAAAG CCTTGCAGCA GCTCCAAGGA 
GAATTAGAGA GTAAGAGCCA ACAGCTTCGT ACCCTGGAGA CTGAAAAATG TAATGAGATC 
AGAGCCCATG AGCAACACAT TCAACACCTA AACCATAGTC TGAGCCACAA AGAGCAGCTG 
CTTCAGGAAT TTCGGGAGCT CCTGCAGTAT CGAGATAAGT CAGACAAAAC CCTTGAAGCA 
AATGAAATGT TGCTTGAGAA ACTTCGGCAG CGAATACAAG ACCGAGATGT TGCTTTGGAG 
CGGGCTATAG ATGAAAAGTT CTCCGCCCTG GAAGAGAAAG AAAAAGAACT GCGTCAATTT 
CATCTTGCTG TGCGAGAGCG TGATCATGAC TTAGAGAGAT TGCGTGCTAT TCTCTCCTCT 
AATGAAGCTA CCATGCAGAG TATGGAGAGT CTCCTGAGGG CCAAAGGCCT AGAAGTAGAG 
CAGTTATCAG CTACCTGTCA AAACCTCCAG TGGCTGAAAG AAGAAAAGGA AACCAAATTT 
AGCCATTGGC AGAAGGAACA AGAAAGTATC ATTCAGCAGT TACAGACATC TCTGCATGAC 
AGGAACAAAG AAGTGGAGGA TCTGAGTGCC ACCTTGTTCT GTAAGCTTGG ACCTGGGCAG 
AGTGAGGTAG CAGAAGAGCT GTGCCAGCGC CTACAGCGAA AGGAAAGGAT GCTACAGGAA 
CTGCTAACCG ATCGAAACAA ACAAGCAGTG GAACACGAAA TAGAGATTCA AGGCCTGCTT 
CAGTCTATGA GCACCAGGGA GCAGGAGAGC CAAGCCGCTG CAGGGAAGAT GGTGCAAGCC 
CTTATGGAAA GAAATTCAGA ACTACAGGCC CTGCGCCAGT ATTTAGGAGG TAAAGACTTC 
ATGGTTTCCC AAGCACTCAT CTCTAACCAA CCCACAGAAG TCACCTCCAA CAGCCCTCGT 
CTTGGAGAGC AGACTGATCA AGGTTCAGTG CATATACCCT CCAAAGATGA TAGCATTTCC 
CTGACTGCCA AAGGGGATAC CAGTATACCC AGGTCCTCAT TAGGAGACCA GGATACAGTT 
GCAGGGCTGG AAAAAGAACT GAGTAATGCC AAAGAGGAGC TTGAGCTCAT GGCTAAGAAA 
GAACGAGAAA GTCGGATGGA ACTTTCTGCA CTACAGTCCA TGGTGGCCGT GCAAGAGGAA 
GAGCTGCAGG TGCAGGCTGC TGACATGGAA TCCCTGACCA GGAATATACA GATTAAAGAA 
GACCTCATAA AGGACCTGCA GATGCAACTG GTTGATCCTG AAGATATACC AGCTATGGAA 
CGCCTGACCC AAGAGGTCTT ACTTCTTCGG GAAAAAGTTG CTTCAGTAGA ATCACAGGGT 
CAGGAAACGT TAGGAAATCG AAGACAACAA TTGCTGCTAA TGCTCGAAGG GCTGGTGGAT 
GAACGGAGTC GGCTCAATGA AGCTTTACAA GCAGAGAGAC AGCTGTACAG CAGCCTGGTG 
AAGTTCCATG CCCATCCAGA GAGCTCTGAG AGAGACCGAA CTCTGCAGGT GGAACTAGAA 
GGGGCCCAGG TGATACGTGG TCGGCTAGAA GAAGTTCTTG GAAGAAGCCT GGAGCGCTTA 
AGCAGGCTGG AGACCCTGGC TGCCATTGGA GGTGCAGCTG CAGGGGAAGA TACAGAAGAT 
GCTAGCACTG AGTTCACGGA CAGCATCGAG GAGGAGGCTG CACACCATAA TCACCAGCAA 
CTCATCAAGG TGGCTTTGGA GAAAAGTCTG GCAACTGTGG AGACTCAGAA CGTATGTCTC 
CCCCCTCCTT CCTCAGCAGG AGGGGACAAT AACAGGGGTC TTCAGGAGGA GATGCTCCAC 
CTGAGGGCTG AGATCCACCA GCACTTAGAG GAGAAGAGGA GAGCTGAGGG GGAACTGAAG 
GAGCTAAAGG CTCAAATTGA GGAAGCAGGA TTCTCCTCTG TGGCCCACAT CAGGAACACC 
ATGCTGAGCC TTTGCCTTGA GAATGCAGAG CTGAAAGAGC AGATGGGAGA AGCAATGTCT 
GATGGATGGG AGATCGAGGA AGACAAGGAG AAGGGCGAGG TGACAGTGGA AACTGTGGCA 
GCCAAAGGAG GTCTGAATGA AAATAGCCTT CAGGCTGAGT TCAGAAAGCT CCAGGGAAAA 
CTGAAGCATG CCCACAACAT TATCAACCTC CTCAAAGAGC AGCTGGTGCT GAGCAGCAAG 
GATGGGAATA GTAAACTTAG TCCAGAGCTC CTAGTGCGCC TGGACCGGGA GATCGACAGA 
ATGAACACAG AGCTGGTCTG TTCTCCGGAG AAGCCCCAGT GCCAGGAAGA AGAGCATATG 
ACCACAAGGC CCTGCCCCAG ACCCCAGAGC CTTGACCTTG GGGCTACCCT CTCAGTGGAT 
GGCCACCAAC TGGATAACCG GCCCCAGGCC CAAGACCCTG GGCCTCAGTC AGAATTTAGC 
CTTCCTGGAT CCACCAAGCA CCTGCGCTCC CAGCTGGCCC AATGCAGACA ACGCTATCAG 
GATCTCCAGG AGAAGCTGCT GATATCGGAA GCCACAGTCT TTGCACAGGC CAACCAACTG 
GAGAAATACC GAGTCATGCT TAGTGAATCC TTGGTGAAGC AGGACAGCAA GCAGGTGCAG 
GTGGACCTCC AGGATCTGGG CTATGAAACC TGTGGCCGAA GTGAGAATGA GGCTGAGCGG 
GAGGAGAGCA CCAGTCCCGA GTGTGAGGAG CCTGACAGCC TCAGGGAAAC AGTCCTGATG 
GTGGGGCACC TGGGCTCACT GCTGGCCAGC CCTCCAGGGA AGAAGCCCTT GGAAAGCCAT 
CGGGGGAAGC AGGAGGAGTT CCAGGCATAT GGAAAGTCAG AAGACATCTC TGTCCTACAT 
AAGGACATCA GAGATCTGAA GGCCCAGCTG CAGAATGCCA ACAAGATCAT TCAGAATCTT 
AAGAGCCGGG TCCGGTCCCT CTCAGTTACA AGTGATTATT CATCTAGTCT GGAGAGACCA 
AGAAAGCTGA AGGCCATTGG CACCCTTGAG GGATCCTCAC CTCACAGTGT CACTGACGAG 
GATGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTGCA CCCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGAGTG TCTCAGCTGG AGGCCCAGCT CCCGAAAACT 
GGAGTGGAAG GGAAGCTAGC AGAGGAGCTG AGATCAGCCT CATGGCCTGG GAAATATGAT 
TCCCTAATTC AGGATCAGGC CCGAGAGCTG TCGTACCTAC GCCAGAAGAT ACGAGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAT GCAAAAGATA CAGTAAAATC TTTTGAGGAC 
CTTCTTAGGA GCAATGACAT TGACTACTAC CTGGGCCAGA GCTTCCGTGA GCAACTTGCC 
CAGGGAAGCC AGCTGACAGA GAGACTCACC AGTAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT TGAACCACTG GCCCTCAGGC TCAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATTGAAGT CCTGCAGGCC AAGCTAGATG CTCGGTCTCT CACACCCTCC 
AGCAGCCATG CATTGTCAGA CTCCCACCGC TCTCCCAGCA GCTCGTCCTT CCTGTCTGAT 
GAGCTGGAAG CCTGCTCTGA CATGGATGTA GCCAGCGAGT ACACACATTA TGAAGAGAAG 
AAGGCTCCAC CTGGTCACTC AGATTCCATC CATCATTTGA GTCATTCTGC TGTACTGTCT 
TCTAAACCAT CAGCAACCAG TGTACCTCGG GGGGTTAAGG CCGAACCCAG CAGCAACCCC 
ATCAGCTTGC CAGCTCCCCA GAACCCCCCC CAAGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTTCCCT CCATATCCAC ACTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCCACTGGC CCTCCCCTCC TTGGCTGCTG TGAGACACCT 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCAGAAACA ATTGGGAGAA 
AGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAG TCCCTAAGGG CTCTGGCAAG CACCCCAGTC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260606.1
CDS	364..7869
Translation	MKESCRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVPRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNEDSGTETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQELHSCHTSEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVARSISCAPSRWSTSICTEEPALSEVGPPDLPSTKVPPDGESMEEGTPGSSVESLDASVQASPPQQKDEETERSAKELAKCDCCSDEQAPQHVCNHKLELALNMIKGLDYKPIQSPRGSKLPIPVKSSPPGARPGHIMTDGVSSGLLNRSLKPLYKIPVNYPLELSDLQELWDDLCEDYLPLRVQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_022404898.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404898.1

ORF Insert Sequence:

ATGAAGGAGA GCTGCAGGAT CTGTGCCCGG GAGCTGTGTG GGAACCAGCG GCGCTGGATC 
TTCCACACGG CATCTAAACT CAACCTCCAG GTTCTGCTCT CGCACGTCCT GGGCAAGGAT 
GTGCCCCGCG ATGGCAAAGC CGAGTTTGCT TGCAGCAAAT GTGCTTTCAT GCTTGATCGG 
ATCTATAGAT TCGACACCGT TATTGCACGG ATCGAAGCTC TTTCTATCGA GCGTTTGCAA 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGAGGACT CTGGCACGGA AACCAAGGCG GGGAATGGGA CCGTTGACAT TTCCGGCTTA 
CCCGACGTAA GATACTCTGC GCTGCTCCAG GAGGACTTTG CCTATTCAGG GTTTGAGTGT 
TGGGTAGAAA ACGAGGATCA GATTCAGGAG CTACACAGCT GCCATACTTC GGAAGGTCCT 
GGAAACCGAC CCAGGAGATG CCGTGGTTGT GCAGCCTTGC GGGTTGCTGA TTCTGACTAT 
GAAGCCATTT GTAAGGTGCC CCGAAAGGTG GCCAGAAGTA TCTCGTGTGC TCCTTCTAGG 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCATTGTCTG AGGTTGGGCC ACCGGATTTA 
CCAAGTACAA AGGTACCCCC AGATGGAGAG AGCATGGAGG AAGGGACACC CGGATCCTCT 
GTGGAATCTT TGGATGCAAG TGTCCAGGCT AGTCCTCCAC AACAAAAGGA TGAGGAAACG 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGTTGTT CAGATGAACA GGCTCCACAG 
CATGTGTGTA ACCACAAGCT AGAGCTAGCT CTTAACATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GTCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAG CCCACCTGGA 
GCCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTCTCCTCAA CAGGTCTTTG 
AAACCCCTTT ACAAGATACC TGTGAATTAT CCCTTGGAGC TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TTTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCTGTGAC TGAAGAGTTA 
CTCAAGCAAC AAAAGCTGGA TTCAGATGAG ACCATTATAA CTCAGCAGTC TGTATCTGAT 
TCCCACTTGG CTGAACTCCA GGAAAAAATC CAGCAAATGG AGGCCACCAA CAAAATTCTT 
CAAGAGAAAC TGAATGAAAT GAGCTGTGAG CTAAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGGTA CAATTCAAAG CCTCAAGGAA ACTCTGAAAA GTAGGGAAAA TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAT GACACAATGG CAAAGCTTCG AGAAATGCTA 
CACCAAAGCC AGCTTGGACA ACTTCATGTA TTTGAGGGTC ACATAGGACA GGAGAGCTCA 
CAGGGTACTT CCCCAGCTCA GCAACAGATG GCTCTGCTTG ATCTTCAGAG TGGTTTGTTC 
TACAGCCAGC TTGAGATTCA GAAACTCCAG AGGTTGGTAC GTCAAAAAGA ACGCCAACTG 
GCTGATGCCA AACGATGTGT CCAATTTGTA GAGGCTGCAG CCCATGAAAG AGAACAGCAG 
AAAGAGGCAT CTTGGAAACA TAATCAGGAA TTACGGAAAG CCTTGCAGCA GCTCCAAGGA 
GAATTAGAGA GTAAGAGCCA ACAGCTTCGT ACCCTGGAGA CTGAAAAATG TAATGAGATC 
AGAGCCCATG AGCAACACAT TCAACACCTA AACCATAGTC TGAGCCACAA AGAGCAGCTG 
CTTCAGGAAT TTCGGGAGCT CCTGCAGTAT CGAGATAAGT CAGACAAAAC CCTTGAAGCA 
AATGAAATGT TGCTTGAGAA ACTTCGGCAG CGAATACAAG ACCGAGATGT TGCTTTGGAG 
CGGGCTATAG ATGAAAAGTT CTCCGCCCTG GAAGAGAAAG AAAAAGAACT GCGTCAATTT 
CATCTTGCTG TGCGAGAGCG TGATCATGAC TTAGAGAGAT TGCGTGCTAT TCTCTCCTCT 
AATGAAGCTA CCATGCAGAG TATGGAGAGT CTCCTGAGGG CCAAAGGCCT AGAAGTAGAG 
CAGTTATCAG CTACCTGTCA AAACCTCCAG TGGCTGAAAG AAGAAAAGGA AACCAAATTT 
AGCCATTGGC AGAAGGAACA AGAAAGTATC ATTCAGCAGT TACAGACATC TCTGCATGAC 
AGGAACAAAG AAGTGGAGGA TCTGAGTGCC ACCTTGTTCT GTAAGCTTGG ACCTGGGCAG 
AGTGAGGTAG CAGAAGAGCT GTGCCAGCGC CTACAGCGAA AGGAAAGGAT GCTACAGGAA 
CTGCTAACCG ATCGAAACAA ACAAGCAGTG GAACACGAAA TAGAGATTCA AGGCCTGCTT 
CAGTCTATGA GCACCAGGGA GCAGGAGAGC CAAGCCGCTG CAGGGAAGAT GGTGCAAGCC 
CTTATGGAAA GAAATTCAGA ACTACAGGCC CTGCGCCAGT ATTTAGGAGG TAAAGACTTC 
ATGGTTTCCC AAGCACTCAT CTCTAACCAA CCCACAGAAG TCACCTCCAA CAGCCCTCGT 
CTTGGAGAGC AGACTGATCA AGGTTCAGTG CATATACCCT CCAAAGATGA TAGCATTTCC 
CTGACTGCCA AAGGGGATAC CAGTATACCC AGGTCCTCAT TAGGAGACCA GGATACAGTT 
GCAGGGCTGG AAAAAGAACT GAGTAATGCC AAAGAGGAGC TTGAGCTCAT GGCTAAGAAA 
GAACGAGAAA GTCGGATGGA ACTTTCTGCA CTACAGTCCA TGGTGGCCGT GCAAGAGGAA 
GAGCTGCAGG TGCAGGCTGC TGACATGGAA TCCCTGACCA GGAATATACA GATTAAAGAA 
GACCTCATAA AGGACCTGCA GATGCAACTG GTTGATCCTG AAGATATACC AGCTATGGAA 
CGCCTGACCC AAGAGGTCTT ACTTCTTCGG GAAAAAGTTG CTTCAGTAGA ATCACAGGGT 
CAGGAAACGT TAGGAAATCG AAGACAACAA TTGCTGCTAA TGCTCGAAGG GCTGGTGGAT 
GAACGGAGTC GGCTCAATGA AGCTTTACAA GCAGAGAGAC AGCTGTACAG CAGCCTGGTG 
AAGTTCCATG CCCATCCAGA GAGCTCTGAG AGAGACCGAA CTCTGCAGGT GGAACTAGAA 
GGGGCCCAGG TGATACGTGG TCGGCTAGAA GAAGTTCTTG GAAGAAGCCT GGAGCGCTTA 
AGCAGGCTGG AGACCCTGGC TGCCATTGGA GGTGCAGCTG CAGGGGAAGA TACAGAAGAT 
GCTAGCACTG AGTTCACGGA CAGCATCGAG GAGGAGGCTG CACACCATAA TCACCAGCAA 
CTCATCAAGG TGGCTTTGGA GAAAAGTCTG GCAACTGTGG AGACTCAGAA CGTATGTCTC 
CCCCCTCCTT CCTCAGCAGG AGGGGACAAT AACAGGGGTC TTCAGGAGGA GATGCTCCAC 
CTGAGGGCTG AGATCCACCA GCACTTAGAG GAGAAGAGGA GAGCTGAGGG GGAACTGAAG 
GAGCTAAAGG CTCAAATTGA GGAAGCAGGA TTCTCCTCTG TGGCCCACAT CAGGAACACC 
ATGCTGAGCC TTTGCCTTGA GAATGCAGAG CTGAAAGAGC AGATGGGAGA AGCAATGTCT 
GATGGATGGG AGATCGAGGA AGACAAGGAG AAGGGCGAGG TGACAGTGGA AACTGTGGCA 
GCCAAAGGAG GTCTGAATGA AAATAGCCTT CAGGCTGAGT TCAGAAAGCT CCAGGGAAAA 
CTGAAGCATG CCCACAACAT TATCAACCTC CTCAAAGAGC AGCTGGTGCT GAGCAGCAAG 
GATGGGAATA GTAAACTTAG TCCAGAGCTC CTAGTGCGCC TGGACCGGGA GATCGACAGA 
ATGAACACAG AGCTGGTCTG TTCTCCGGAG AAGCCCCAGT GCCAGGAAGA AGAGCATATG 
ACCACAAGGC CCTGCCCCAG ACCCCAGAGC CTTGACCTTG GGGCTACCCT CTCAGTGGAT 
GGCCACCAAC TGGATAACCG GCCCCAGGCC CAAGACCCTG GGCCTCAGTC AGAATTTAGC 
CTTCCTGGAT CCACCAAGCA CCTGCGCTCC CAGCTGGCCC AATGCAGACA ACGCTATCAG 
GATCTCCAGG AGAAGCTGCT GATATCGGAA GCCACAGTCT TTGCACAGGC CAACCAACTG 
GAGAAATACC GAGTCATGCT TAGTGAATCC TTGGTGAAGC AGGACAGCAA GCAGGTGCAG 
GTGGACCTCC AGGATCTGGG CTATGAAACC TGTGGCCGAA GTGAGAATGA GGCTGAGCGG 
GAGGAGAGCA CCAGTCCCGA GTGTGAGGAG CCTGACAGCC TCAGGGAAAC AGTCCTGATG 
GTGGGGCACC TGGGCTCACT GCTGGCCAGC CCTCCAGGGA AGAAGCCCTT GGAAAGCCAT 
CGGGGGAAGC AGGAGGAGTT CCAGGCATAT GGAAAGTCAG AAGACATCTC TGTCCTACAT 
AAGGACATCA GAGATCTGAA GGCCCAGCTG CAGAATGCCA ACAAGATCAT TCAGAATCTT 
AAGAGCCGGG TCCGGTCCCT CTCAGTTACA AGTGATTATT CATCTAGTCT GGAGAGACCA 
AGAAAGCTGA AGGCCATTGG CACCCTTGAG GGATCCTCAC CTCACAGTGT CACTGACGAG 
GATGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTGCA CCCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGAGTG TCTCAGCTGG AGGCCCAGCT CCCGAAAACT 
GGAGTGGAAG GGAAGCTAGC AGAGGAGCTG AGATCAGCCT CATGGCCTGG GAAATATGAT 
TCCCTAATTC AGGATCAGGC CCGAGAGCTG TCGTACCTAC GCCAGAAGAT ACGAGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAT GCAAAAGATA CAGTAAAATC TTTTGAGGAC 
CTTCTTAGGA GCAATGACAT TGACTACTAC CTGGGCCAGA GCTTCCGTGA GCAACTTGCC 
CAGGGAAGCC AGCTGACAGA GAGACTCACC AGTAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT TGAACCACTG GCCCTCAGGC TCAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATTGAAGT CCTGCAGGCC AAGCTAGATG CTCGGTCTCT CACACCCTCC 
AGCAGCCATG CATTGTCAGA CTCCCACCGC TCTCCCAGCA GCTCGTCCTT CCTGTCTGAT 
GAGCTGGAAG CCTGCTCTGA CATGGATGTA GCCAGCGAGT ACACACATTA TGAAGAGAAG 
AAGGCTCCAC CTGGTCACTC AGATTCCATC CATCATTTGA GTCATTCTGC TGTACTGTCT 
TCTAAACCAT CAGCAACCAG TGTACCTCGG GGGGTTAAGG CCGAACCCAG CAGCAACCCC 
ATCAGCTTGC CAGCTCCCCA GAACCCCCCC CAAGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTTCCCT CCATATCCAC ACTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCCACTGGC CCTCCCCTCC TTGGCTGCTG TGAGACACCT 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCAGAAACA ATTGGGAGAA 
AGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAG TCCCTAAGGG CTCTGGCAAG CACCCCAGTC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120599.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976074.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X13, mRNA.

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CloneID	OCe40885
Clone ID Related Accession (Same CDS sequence)	XM_014120599.1 , XM_014120599.2
Accession Version	XM_014120599.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGA CGTGGACCAG AGATTACTTT GCAGAGGATG AAGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACTAAAGATC GAGGCCCTCC AGTGCAATCA 
CAGATCTGGC GAAGTGGTGA AAAGGCCTCC TTTGGACAGA CGCCTTCCTT GAGAGCATTT 
GAAAAACCCC CTCAGGTGCA GACCCAGGCT CTTAGAGACT TTGAGAAGCA TCTCAATGAC 
CTGAAGAAGG AGAACTTCAG CCTCAAGCTG CGCATCTATT TCCTGGAGGA ACGCATGCAG 
CAGAAGTATG AGGCCAGCCG GGAGGACATC TACAAGCGGA ACATCGAGCT GAAGGTTGAA 
GTAGAGAGCC TGAAACGGGA ACTCCAGGAC AAGAAACAGC ATCTAGATAA AACGTGGGCT 
GATGTGGAGA ATCTCAACAG CCATAATGAA GCTGAGCTCC GGCGTCAGTT TGAAGAGAGA 
CAGCAGGAGA CGGAGCATGT TTACGAGCTC CTGGAGAATA AGATCCAGCT TCTGCAGGAG 
GAATCCAGGC TAGCAAAGAA TGAGGCTGCA CGGATGGCAG CTCTGGTGGA AGCTGAGAAA 
GAATGTAACC TGGAGCTATC AGAGAAACTG AAGGGAGTTG CCAATGACAG GGAAGAGGTA 
CCAATGGACC AGGTCAATCC TGACCAATAC ACTGAGGCCC TGGCCCAGCG GGACAAAAGA 
ATTGAAGAAC TTCATCAAAG CCTGGCTTCC CAGGAGAAGC TTGTAGAACA GCTGTCTCAA 
GAGAAACAAC AACTGCTCCA TCTGTTGGAG GAACCAATGA GCATGGAAAT ACAGCCTGTG 
ACTGAAGAGT TACTCAAGCA ACAAAAGCTG GATTCAGATG AGACCATTAT AACTCAGCAG 
TCTGTATCTG ATTCCCACTT GGCTGAACTC CAGGAAAAAA TCCAGCAAAT GGAGGCCACC 
AACAAAATTC TTCAAGAGAA ACTGAATGAA ATGAGCTGTG AGCTAAAGTC TGCTCAGGAG 
TCATCTCAGA AGCAAGATGG TACAATTCAA AGCCTCAAGG AAACTCTGAA AAGTAGGGAA 
AATGAGACCG AGGAGTTGTA CCAGGTGATT GAAGGTCAAA ATGACACAAT GGCAAAGCTT 
CGAGAAATGC TACACCAAAG CCAGCTTGGA CAACTTCATG TATTTGAGGG TCACATAGGA 
CAGGAGAGCT CACAGGGTAC TTCCCCAGCT CAGCAACAGA TGGCTCTGCT TGATCTTCAG 
AGTGGTTTGT TCTACAGCCA GCTTGAGATT CAGAAACTCC AGAGGTTGGT ACGTCAAAAA 
GAACGCCAAC TGGCTGATGC CAAACGATGT GTCCAATTTG TAGAGGCTGC AGCCCATGAA 
AGAGAACAGC AGAAAGAGGC ATCTTGGAAA CATAATCAGG AATTACGGAA AGCCTTGCAG 
CAGCTCCAAG GAGAATTAGA GAGTAAGAGC CAACAGCTTC GTACCCTGGA GACTGAAAAA 
TGTAATGAGA TCAGAGCCCA TGAGCAACAC ATTCAACACC TAAACCATAG TCTGAGCCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ATCGAGATAA GTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTCGGC AGCGAATACA AGACCGAGAT 
GTTGCTTTGG AGCGGGCTAT AGATGAAAAG TTCTCCGCCC TGGAAGAGAA AGAAAAAGAA 
CTGCGTCAAT TTCATCTTGC TGTGCGAGAG CGTGATCATG ACTTAGAGAG ATTGCGTGCT 
ATTCTCTCCT CTAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GGCCAAAGGC 
CTAGAAGTAG AGCAGTTATC AGCTACCTGT CAAAACCTCC AGTGGCTGAA AGAAGAAAAG 
GAAACCAAAT TTAGCCATTG GCAGAAGGAA CAAGAAAGTA TCATTCAGCA GTTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTGAGTG CCACCTTGTT CTGTAAGCTT 
GGACCTGGGC AGAGTGAGGT AGCAGAAGAG CTGTGCCAGC GCCTACAGCG AAAGGAAAGG 
ATGCTACAGG AACTGCTAAC CGATCGAAAC AAACAAGCAG TGGAACACGA AATAGAGATT 
CAAGGCCTGC TTCAGTCTAT GAGCACCAGG GAGCAGGAGA GCCAAGCCGC TGCAGGGAAG 
ATGGTGCAAG CCCTTATGGA AAGAAATTCA GAACTACAGG CCCTGCGCCA GTATTTAGGA 
GGTAAAGACT TCATGGTTTC CCAAGCACTC ATCTCTAACC AACCCACAGA AGTCACCTCC 
AACAGCCCTC GTCTTGGAGA GCAGACTGAT CAAGGTTCAG TGCATATACC CTCCAAAGAT 
GATAGCATTT CCCTGACTGC CAAAGGGGAT ACCAGTATAC CCAGGTCCTC ATTAGGAGAC 
CAGGATACAG TTGCAGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA GCTTGAGCTC 
ATGGCTAAGA AAGAACGAGA AAGTCGGATG GAACTTTCTG CACTACAGTC CATGGTGGCC 
GTGCAAGAGG AAGAGCTGCA GGTGCAGGCT GCTGACATGG AATCCCTGAC CAGGAATATA 
CAGATTAAAG AAGACCTCAT AAAGGACCTG CAGATGCAAC TGGTTGATCC TGAAGATATA 
CCAGCTATGG AACGCCTGAC CCAAGAGGTC TTACTTCTTC GGGAAAAAGT TGCTTCAGTA 
GAATCACAGG GTCAGGAAAC GTTAGGAAAT CGAAGACAAC AATTGCTGCT AATGCTCGAA 
GGGCTGGTGG ATGAACGGAG TCGGCTCAAT GAAGCTTTAC AAGCAGAGAG ACAGCTGTAC 
AGCAGCCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGT GGTCGGCTAG AAGAAGTTCT TGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCTGCCATTG GAGGTGCAGC TGCAGGGGAA 
GATACAGAAG ATGCTAGCAC TGAGTTCACG GACAGCATCG AGGAGGAGGC TGCACACCAT 
AATCACCAGC AACTCATCAA GGTGGCTTTG GAGAAAAGTC TGGCAACTGT GGAGACTCAG 
AACGTATGTC TCCCCCCTCC TTCCTCAGCA GGAGGGGACA ATAACAGGGG TCTTCAGGAG 
GAGATGCTCC ACCTGAGGGC TGAGATCCAC CAGCACTTAG AGGAGAAGAG GAGAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATCGAG GAAGACAAGG AGAAGGGCGA GGTGACAGTG 
GAAACTGTGG CAGCCAAAGG AGGTCTGAAT GAAAATAGCC TTCAGGCTGA GTTCAGAAAG 
CTCCAGGGAA AACTGAAGCA TGCCCACAAC ATTATCAACC TCCTCAAAGA GCAGCTGGTG 
CTGAGCAGCA AGGATGGGAA TAGTAAACTT AGTCCAGAGC TCCTAGTGCG CCTGGACCGG 
GAGATCGACA GAATGAACAC AGAGCTGGTC TGTTCTCCGG AGAAGCCCCA GTGCCAGGAA 
GAAGAGCATA TGACCACAAG GCCCTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTACC 
CTCTCAGTGG ATGGCCACCA ACTGGATAAC CGGCCCCAGG CCCAAGACCC TGGGCCTCAG 
TCAGAATTTA GCCTTCCTGG ATCCACCAAG CACCTGCGCT CCCAGCTGGC CCAATGCAGA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCGG AAGCCACAGT CTTTGCACAG 
GCCAACCAAC TGGAGAAATA CCGAGTCATG CTTAGTGAAT CCTTGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGATCTG GGCTATGAAA CCTGTGGCCG AAGTGAGAAT 
GAGGCTGAGC GGGAGGAGAG CACCAGTCCC GAGTGTGAGG AGCCTGACAG CCTCAGGGAA 
ACAGTCCTGA TGGTGGGGCA CCTGGGCTCA CTGCTGGCCA GCCCTCCAGG GAAGAAGCCC 
TTGGAAAGCC ATCGGGGGAA GCAGGAGGAG TTCCAGGCAT ATGGAAAGTC AGAAGACATC 
TCTGTCCTAC ATAAGGACAT CAGAGATCTG AAGGCCCAGC TGCAGAATGC CAACAAGATC 
ATTCAGAATC TTAAGAGCCG GGTCCGGTCC CTCTCAGTTA CAAGTGATTA TTCATCTAGT 
CTGGAGAGAC CAAGAAAGCT GAAGGCCATT GGCACCCTTG AGGGATCCTC ACCTCACAGT 
GTCACTGACG AGGATGAGGG GTGGCTGTCT GATGGCACTG GGGCTTTCTG CACCCCAGGG 
CTTCAGGCTA AAAAGGATCT GGAGAGTCTG ATCCAGAGAG TGTCTCAGCT GGAGGCCCAG 
CTCCCGAAAA CTGGAGTGGA AGGGAAGCTA GCAGAGGAGC TGAGATCAGC CTCATGGCCT 
GGGAAATATG ATTCCCTAAT TCAGGATCAG GCCCGAGAGC TGTCGTACCT ACGCCAGAAG 
ATACGAGAAG GGAGAGGTAT CTGTTATCTT CTCACCCAGC ATGCAAAAGA TACAGTAAAA 
TCTTTTGAGG ACCTTCTTAG GAGCAATGAC ATTGACTACT ACCTGGGCCA GAGCTTCCGT 
GAGCAACTTG CCCAGGGAAG CCAGCTGACA GAGAGACTCA CCAGTAAACT CAGCACCAAG 
GATCATAAAA GTGAGAAAGA TCAAGCTGGA CTTGAACCAC TGGCCCTCAG GCTCAGCAGG 
GAGCTGCAGG AGAAGGAGAA AGTGATTGAA GTCCTGCAGG CCAAGCTAGA TGCTCGGTCT 
CTCACACCCT CCAGCAGCCA TGCATTGTCA GACTCCCACC GCTCTCCCAG CAGCTCGTCC 
TTCCTGTCTG ATGAGCTGGA AGCCTGCTCT GACATGGATG TAGCCAGCGA GTACACACAT 
TATGAAGAGA AGAAGGCTCC ACCTGGTCAC TCAGATTCCA TCCATCATTT GAGTCATTCT 
GCTGTACTGT CTTCTAAACC ATCAGCAACC AGTGTACCTC GGGGGGTTAA GGCCGAACCC 
AGCAGCAACC CCATCAGCTT GCCAGCTCCC CAGAACCCCC CCCAAGAGGC CAGCCAGGCC 
CATCCAGGCC TTCATTTTCC CTCCATATCC ACACTGGTTA GCCTTCCCCA GGCACCACTG 
CCCTCAGCTC CACCCAGCTT CCTGCCTTTC AGCTCCACTG GCCCTCCCCT CCTTGGCTGC 
TGTGAGACAC CTATGGTCTC CTTGGCAGAG GCTCAGCAGG AGCTGCAGAT GCTGCAGAAA 
CAATTGGGAG AAAGTGTCAG CACCGTCCAT CCTGCTTCCC CAGCTGCATT GCCAAGCAAC 
CATTTAGAAG CCAACTCTTC CCAATATCTC AACCCTGCCC AGCCCCACTC TCCTCCAACT 
GGCAGCACAG AATTGGGCAG AATCCTGGAG CCTGGCTACC TGGGTAGCGG CAGCCAGTGG 
GATGTGATGA GGCCTCAGAA GGGGAGTGTA TCAGGCGACT TATCCTCAGG ATCCTCTGTG 
TGTCAGCTTA ACTCCAAACC CACAGGGGCT GACCTGCTAG AAGAACATCT TGGTGAAATC 
CGGAACCTGC GCCAGCGCCT AGAGGAGTCC ATCTGCATCA ATGACCGCCT GCGGGAACAG 
CTGGAACACC GGCTCAGCTG CACAGCACGT GGCAATGGAT CCACCTCTAA TTTCTACAGT 
CCTGGCCTGG AACCCACACC TCAGCTCTAC AATGAGAACA GAGTCCTTAA GGAAGAAAAC 
CGGAGCCTTC AGGCTCAGCT GAGTCATGTT TCTAGAGAGC GCTCCCAGGA GACAGAATGC 
CTGAGGGAGG CTCTACTGTC CTCTCAATCC CGCCTTCAAG AGCTGGAAAT GGAGCTAGAG 
CACCAAAAGG AGGAACGGCA GCAGCTTTTG GAAGACTTGA AGGAGAAGCA GCAGGAGATC 
TTGCACTTCC AGGAGGAGCG CCTGTCCCTC CAGGAGAAAG ACTCTAGGCT GCAGCGTAAG 
CTGGCCCTAC TGCAGCAACA GTGTGAAGAG AAACAGCAGC TCTTCCAGTC CCTCCAGTCA 
GAGCTACAGA TCTATGAGGC GCTTTATGGC AGTTCCAAGA AGGGTCTGAA AGCTTCCACT 
TGGGATGCCT ATCACCAGGT CCCTTTGAGC AGTGACTTGA GCCACCTGGT GGCAGAGATC 
CGAGCTTTGC GAGGGCAGCT GGAGCAAAGC ATACAGGTGA ATAACTGTCT GCGACTGCAG 
CTGGAGCAGC AGCTGGATGG CGGAAGGAGC AAAACCAGCC TCAGTCCCTC TTCCACCAGC 
CAGGAGGACC CTGGAGACAA GCAAGACTCA ATTGCTTCCC CTCCAGTCCG AGATGTTGGC 
ATGAATTCTC CAGCTCTCAT GTTCCCTAGC TCTTCTCCTG CTGCTCCAGG CTCAGAGACA 
GCTATTTTCA ATAGGATAAA TGAGCTGAAT TTGGATGCTT TTCCAGTAAT GAAGAACTCT 
CCCAAGCTGG AGGGCGAAGC TATCGATGGC TCCTTTGCCA ATAAGCACGG CCGCCATGTG 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGACAGCAGA TTGGTGAGGG CAAAGTGCTG 
GTCAAAAAGA TAACAGCACT TGTGAGATCA GCCTACACCC TCCCTGGCCT TGAAACTCAG 
GCGACAGAGG TGCCAGGTGG CAAAGGCATC CATGAACTTG GGAGCAGCAC CCAAGCCCTG 
CAGCACGTGC TGGAGGAGTC GGCCTCCCTC CTCAATATGT TCTGGCGAGC GGCCCTGCCA 
AGCTCCAGCC CTGCCCTGTC TGGCAACATG GGACAGGCCA TGAAAAAGGA ACTTCTGGAA 
CTGAGAACCA AACTGACCAA ACAGGAGACC CTCCTTCAGT GCACAGCTGA GCGTCTAAAG 
ACCACCCACC AGCAGAAGGA GAGCATGGAG CAGTTCATCT TCAGCCATTT GACCAGGACA 
CATGATGTTT TGAAGAAGGC AAGGACTAAT TTGGAGGCCA AGTCAGGAAT CCGAGGAAAG 
CAAAAGAACA CTTACAAGAT GGCCTCTGTA AAGCCTTATT CCTGCCCCAG CAAAGGAGCC 
ACGCAAGAAG AGGAGCCCCC AGAGGTCCTT CAAACAGCAG GAAGGATGGG CCTGCCCCCC 
TTTTGTGCAG GTCCCAATCT GCTGAGGAGG CTTGGACCCC ACTCCTCCGC ACTGGCTGGA 
GGGTCCAGGA GGAGTCAGGC ATGGCCCAGT GGACGAGGGG GAAAGGAAGG CAGCCTTTCC 
TGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976074.1
CDS	350..7798
Translation	MEQTWTRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120599.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120599.1

ORF Insert Sequence:

ATGGAGCAGA CGTGGACCAG AGATTACTTT GCAGAGGATG AAGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACTAAAGATC GAGGCCCTCC AGTGCAATCA 
CAGATCTGGC GAAGTGGTGA AAAGGCCTCC TTTGGACAGA CGCCTTCCTT GAGAGCATTT 
GAAAAACCCC CTCAGGTGCA GACCCAGGCT CTTAGAGACT TTGAGAAGCA TCTCAATGAC 
CTGAAGAAGG AGAACTTCAG CCTCAAGCTG CGCATCTATT TCCTGGAGGA ACGCATGCAG 
CAGAAGTATG AGGCCAGCCG GGAGGACATC TACAAGCGGA ACATCGAGCT GAAGGTTGAA 
GTAGAGAGCC TGAAACGGGA ACTCCAGGAC AAGAAACAGC ATCTAGATAA AACGTGGGCT 
GATGTGGAGA ATCTCAACAG CCATAATGAA GCTGAGCTCC GGCGTCAGTT TGAAGAGAGA 
CAGCAGGAGA CGGAGCATGT TTACGAGCTC CTGGAGAATA AGATCCAGCT TCTGCAGGAG 
GAATCCAGGC TAGCAAAGAA TGAGGCTGCA CGGATGGCAG CTCTGGTGGA AGCTGAGAAA 
GAATGTAACC TGGAGCTATC AGAGAAACTG AAGGGAGTTG CCAATGACAG GGAAGAGGTA 
CCAATGGACC AGGTCAATCC TGACCAATAC ACTGAGGCCC TGGCCCAGCG GGACAAAAGA 
ATTGAAGAAC TTCATCAAAG CCTGGCTTCC CAGGAGAAGC TTGTAGAACA GCTGTCTCAA 
GAGAAACAAC AACTGCTCCA TCTGTTGGAG GAACCAATGA GCATGGAAAT ACAGCCTGTG 
ACTGAAGAGT TACTCAAGCA ACAAAAGCTG GATTCAGATG AGACCATTAT AACTCAGCAG 
TCTGTATCTG ATTCCCACTT GGCTGAACTC CAGGAAAAAA TCCAGCAAAT GGAGGCCACC 
AACAAAATTC TTCAAGAGAA ACTGAATGAA ATGAGCTGTG AGCTAAAGTC TGCTCAGGAG 
TCATCTCAGA AGCAAGATGG TACAATTCAA AGCCTCAAGG AAACTCTGAA AAGTAGGGAA 
AATGAGACCG AGGAGTTGTA CCAGGTGATT GAAGGTCAAA ATGACACAAT GGCAAAGCTT 
CGAGAAATGC TACACCAAAG CCAGCTTGGA CAACTTCATG TATTTGAGGG TCACATAGGA 
CAGGAGAGCT CACAGGGTAC TTCCCCAGCT CAGCAACAGA TGGCTCTGCT TGATCTTCAG 
AGTGGTTTGT TCTACAGCCA GCTTGAGATT CAGAAACTCC AGAGGTTGGT ACGTCAAAAA 
GAACGCCAAC TGGCTGATGC CAAACGATGT GTCCAATTTG TAGAGGCTGC AGCCCATGAA 
AGAGAACAGC AGAAAGAGGC ATCTTGGAAA CATAATCAGG AATTACGGAA AGCCTTGCAG 
CAGCTCCAAG GAGAATTAGA GAGTAAGAGC CAACAGCTTC GTACCCTGGA GACTGAAAAA 
TGTAATGAGA TCAGAGCCCA TGAGCAACAC ATTCAACACC TAAACCATAG TCTGAGCCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ATCGAGATAA GTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTCGGC AGCGAATACA AGACCGAGAT 
GTTGCTTTGG AGCGGGCTAT AGATGAAAAG TTCTCCGCCC TGGAAGAGAA AGAAAAAGAA 
CTGCGTCAAT TTCATCTTGC TGTGCGAGAG CGTGATCATG ACTTAGAGAG ATTGCGTGCT 
ATTCTCTCCT CTAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GGCCAAAGGC 
CTAGAAGTAG AGCAGTTATC AGCTACCTGT CAAAACCTCC AGTGGCTGAA AGAAGAAAAG 
GAAACCAAAT TTAGCCATTG GCAGAAGGAA CAAGAAAGTA TCATTCAGCA GTTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTGAGTG CCACCTTGTT CTGTAAGCTT 
GGACCTGGGC AGAGTGAGGT AGCAGAAGAG CTGTGCCAGC GCCTACAGCG AAAGGAAAGG 
ATGCTACAGG AACTGCTAAC CGATCGAAAC AAACAAGCAG TGGAACACGA AATAGAGATT 
CAAGGCCTGC TTCAGTCTAT GAGCACCAGG GAGCAGGAGA GCCAAGCCGC TGCAGGGAAG 
ATGGTGCAAG CCCTTATGGA AAGAAATTCA GAACTACAGG CCCTGCGCCA GTATTTAGGA 
GGTAAAGACT TCATGGTTTC CCAAGCACTC ATCTCTAACC AACCCACAGA AGTCACCTCC 
AACAGCCCTC GTCTTGGAGA GCAGACTGAT CAAGGTTCAG TGCATATACC CTCCAAAGAT 
GATAGCATTT CCCTGACTGC CAAAGGGGAT ACCAGTATAC CCAGGTCCTC ATTAGGAGAC 
CAGGATACAG TTGCAGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA GCTTGAGCTC 
ATGGCTAAGA AAGAACGAGA AAGTCGGATG GAACTTTCTG CACTACAGTC CATGGTGGCC 
GTGCAAGAGG AAGAGCTGCA GGTGCAGGCT GCTGACATGG AATCCCTGAC CAGGAATATA 
CAGATTAAAG AAGACCTCAT AAAGGACCTG CAGATGCAAC TGGTTGATCC TGAAGATATA 
CCAGCTATGG AACGCCTGAC CCAAGAGGTC TTACTTCTTC GGGAAAAAGT TGCTTCAGTA 
GAATCACAGG GTCAGGAAAC GTTAGGAAAT CGAAGACAAC AATTGCTGCT AATGCTCGAA 
GGGCTGGTGG ATGAACGGAG TCGGCTCAAT GAAGCTTTAC AAGCAGAGAG ACAGCTGTAC 
AGCAGCCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGT GGTCGGCTAG AAGAAGTTCT TGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCTGCCATTG GAGGTGCAGC TGCAGGGGAA 
GATACAGAAG ATGCTAGCAC TGAGTTCACG GACAGCATCG AGGAGGAGGC TGCACACCAT 
AATCACCAGC AACTCATCAA GGTGGCTTTG GAGAAAAGTC TGGCAACTGT GGAGACTCAG 
AACGTATGTC TCCCCCCTCC TTCCTCAGCA GGAGGGGACA ATAACAGGGG TCTTCAGGAG 
GAGATGCTCC ACCTGAGGGC TGAGATCCAC CAGCACTTAG AGGAGAAGAG GAGAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATCGAG GAAGACAAGG AGAAGGGCGA GGTGACAGTG 
GAAACTGTGG CAGCCAAAGG AGGTCTGAAT GAAAATAGCC TTCAGGCTGA GTTCAGAAAG 
CTCCAGGGAA AACTGAAGCA TGCCCACAAC ATTATCAACC TCCTCAAAGA GCAGCTGGTG 
CTGAGCAGCA AGGATGGGAA TAGTAAACTT AGTCCAGAGC TCCTAGTGCG CCTGGACCGG 
GAGATCGACA GAATGAACAC AGAGCTGGTC TGTTCTCCGG AGAAGCCCCA GTGCCAGGAA 
GAAGAGCATA TGACCACAAG GCCCTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTACC 
CTCTCAGTGG ATGGCCACCA ACTGGATAAC CGGCCCCAGG CCCAAGACCC TGGGCCTCAG 
TCAGAATTTA GCCTTCCTGG ATCCACCAAG CACCTGCGCT CCCAGCTGGC CCAATGCAGA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCGG AAGCCACAGT CTTTGCACAG 
GCCAACCAAC TGGAGAAATA CCGAGTCATG CTTAGTGAAT CCTTGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGATCTG GGCTATGAAA CCTGTGGCCG AAGTGAGAAT 
GAGGCTGAGC GGGAGGAGAG CACCAGTCCC GAGTGTGAGG AGCCTGACAG CCTCAGGGAA 
ACAGTCCTGA TGGTGGGGCA CCTGGGCTCA CTGCTGGCCA GCCCTCCAGG GAAGAAGCCC 
TTGGAAAGCC ATCGGGGGAA GCAGGAGGAG TTCCAGGCAT ATGGAAAGTC AGAAGACATC 
TCTGTCCTAC ATAAGGACAT CAGAGATCTG AAGGCCCAGC TGCAGAATGC CAACAAGATC 
ATTCAGAATC TTAAGAGCCG GGTCCGGTCC CTCTCAGTTA CAAGTGATTA TTCATCTAGT 
CTGGAGAGAC CAAGAAAGCT GAAGGCCATT GGCACCCTTG AGGGATCCTC ACCTCACAGT 
GTCACTGACG AGGATGAGGG GTGGCTGTCT GATGGCACTG GGGCTTTCTG CACCCCAGGG 
CTTCAGGCTA AAAAGGATCT GGAGAGTCTG ATCCAGAGAG TGTCTCAGCT GGAGGCCCAG 
CTCCCGAAAA CTGGAGTGGA AGGGAAGCTA GCAGAGGAGC TGAGATCAGC CTCATGGCCT 
GGGAAATATG ATTCCCTAAT TCAGGATCAG GCCCGAGAGC TGTCGTACCT ACGCCAGAAG 
ATACGAGAAG GGAGAGGTAT CTGTTATCTT CTCACCCAGC ATGCAAAAGA TACAGTAAAA 
TCTTTTGAGG ACCTTCTTAG GAGCAATGAC ATTGACTACT ACCTGGGCCA GAGCTTCCGT 
GAGCAACTTG CCCAGGGAAG CCAGCTGACA GAGAGACTCA CCAGTAAACT CAGCACCAAG 
GATCATAAAA GTGAGAAAGA TCAAGCTGGA CTTGAACCAC TGGCCCTCAG GCTCAGCAGG 
GAGCTGCAGG AGAAGGAGAA AGTGATTGAA GTCCTGCAGG CCAAGCTAGA TGCTCGGTCT 
CTCACACCCT CCAGCAGCCA TGCATTGTCA GACTCCCACC GCTCTCCCAG CAGCTCGTCC 
TTCCTGTCTG ATGAGCTGGA AGCCTGCTCT GACATGGATG TAGCCAGCGA GTACACACAT 
TATGAAGAGA AGAAGGCTCC ACCTGGTCAC TCAGATTCCA TCCATCATTT GAGTCATTCT 
GCTGTACTGT CTTCTAAACC ATCAGCAACC AGTGTACCTC GGGGGGTTAA GGCCGAACCC 
AGCAGCAACC CCATCAGCTT GCCAGCTCCC CAGAACCCCC CCCAAGAGGC CAGCCAGGCC 
CATCCAGGCC TTCATTTTCC CTCCATATCC ACACTGGTTA GCCTTCCCCA GGCACCACTG 
CCCTCAGCTC CACCCAGCTT CCTGCCTTTC AGCTCCACTG GCCCTCCCCT CCTTGGCTGC 
TGTGAGACAC CTATGGTCTC CTTGGCAGAG GCTCAGCAGG AGCTGCAGAT GCTGCAGAAA 
CAATTGGGAG AAAGTGTCAG CACCGTCCAT CCTGCTTCCC CAGCTGCATT GCCAAGCAAC 
CATTTAGAAG CCAACTCTTC CCAATATCTC AACCCTGCCC AGCCCCACTC TCCTCCAACT 
GGCAGCACAG AATTGGGCAG AATCCTGGAG CCTGGCTACC TGGGTAGCGG CAGCCAGTGG 
GATGTGATGA GGCCTCAGAA GGGGAGTGTA TCAGGCGACT TATCCTCAGG ATCCTCTGTG 
TGTCAGCTTA ACTCCAAACC CACAGGGGCT GACCTGCTAG AAGAACATCT TGGTGAAATC 
CGGAACCTGC GCCAGCGCCT AGAGGAGTCC ATCTGCATCA ATGACCGCCT GCGGGAACAG 
CTGGAACACC GGCTCAGCTG CACAGCACGT GGCAATGGAT CCACCTCTAA TTTCTACAGT 
CCTGGCCTGG AACCCACACC TCAGCTCTAC AATGAGAACA GAGTCCTTAA GGAAGAAAAC 
CGGAGCCTTC AGGCTCAGCT GAGTCATGTT TCTAGAGAGC GCTCCCAGGA GACAGAATGC 
CTGAGGGAGG CTCTACTGTC CTCTCAATCC CGCCTTCAAG AGCTGGAAAT GGAGCTAGAG 
CACCAAAAGG AGGAACGGCA GCAGCTTTTG GAAGACTTGA AGGAGAAGCA GCAGGAGATC 
TTGCACTTCC AGGAGGAGCG CCTGTCCCTC CAGGAGAAAG ACTCTAGGCT GCAGCGTAAG 
CTGGCCCTAC TGCAGCAACA GTGTGAAGAG AAACAGCAGC TCTTCCAGTC CCTCCAGTCA 
GAGCTACAGA TCTATGAGGC GCTTTATGGC AGTTCCAAGA AGGGTCTGAA AGCTTCCACT 
TGGGATGCCT ATCACCAGGT CCCTTTGAGC AGTGACTTGA GCCACCTGGT GGCAGAGATC 
CGAGCTTTGC GAGGGCAGCT GGAGCAAAGC ATACAGGTGA ATAACTGTCT GCGACTGCAG 
CTGGAGCAGC AGCTGGATGG CGGAAGGAGC AAAACCAGCC TCAGTCCCTC TTCCACCAGC 
CAGGAGGACC CTGGAGACAA GCAAGACTCA ATTGCTTCCC CTCCAGTCCG AGATGTTGGC 
ATGAATTCTC CAGCTCTCAT GTTCCCTAGC TCTTCTCCTG CTGCTCCAGG CTCAGAGACA 
GCTATTTTCA ATAGGATAAA TGAGCTGAAT TTGGATGCTT TTCCAGTAAT GAAGAACTCT 
CCCAAGCTGG AGGGCGAAGC TATCGATGGC TCCTTTGCCA ATAAGCACGG CCGCCATGTG 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGACAGCAGA TTGGTGAGGG CAAAGTGCTG 
GTCAAAAAGA TAACAGCACT TGTGAGATCA GCCTACACCC TCCCTGGCCT TGAAACTCAG 
GCGACAGAGG TGCCAGGTGG CAAAGGCATC CATGAACTTG GGAGCAGCAC CCAAGCCCTG 
CAGCACGTGC TGGAGGAGTC GGCCTCCCTC CTCAATATGT TCTGGCGAGC GGCCCTGCCA 
AGCTCCAGCC CTGCCCTGTC TGGCAACATG GGACAGGCCA TGAAAAAGGA ACTTCTGGAA 
CTGAGAACCA AACTGACCAA ACAGGAGACC CTCCTTCAGT GCACAGCTGA GCGTCTAAAG 
ACCACCCACC AGCAGAAGGA GAGCATGGAG CAGTTCATCT TCAGCCATTT GACCAGGACA 
CATGATGTTT TGAAGAAGGC AAGGACTAAT TTGGAGGCCA AGTCAGGAAT CCGAGGAAAG 
CAAAAGAACA CTTACAAGAT GGCCTCTGTA AAGCCTTATT CCTGCCCCAG CAAAGGAGCC 
ACGCAAGAAG AGGAGCCCCC AGAGGTCCTT CAAACAGCAG GAAGGATGGG CCTGCCCCCC 
TTTTGTGCAG GTCCCAATCT GCTGAGGAGG CTTGGACCCC ACTCCTCCGC ACTGGCTGGA 
GGGTCCAGGA GGAGTCAGGC ATGGCCCAGT GGACGAGGGG GAAAGGAAGG CAGCCTTTCC 
TGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120599.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976074.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X13, mRNA.

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CloneID	OCe40885
Clone ID Related Accession (Same CDS sequence)	XM_014120599.1 , XM_014120599.2
Accession Version	XM_014120599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGA CGTGGACCAG AGATTACTTT GCAGAGGATG AAGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACTAAAGATC GAGGCCCTCC AGTGCAATCA 
CAGATCTGGC GAAGTGGTGA AAAGGCCTCC TTTGGACAGA CGCCTTCCTT GAGAGCATTT 
GAAAAACCCC CTCAGGTGCA GACCCAGGCT CTTAGAGACT TTGAGAAGCA TCTCAATGAC 
CTGAAGAAGG AGAACTTCAG CCTCAAGCTG CGCATCTATT TCCTGGAGGA ACGCATGCAG 
CAGAAGTATG AGGCCAGCCG GGAGGACATC TACAAGCGGA ACATCGAGCT GAAGGTTGAA 
GTAGAGAGCC TGAAACGGGA ACTCCAGGAC AAGAAACAGC ATCTAGATAA AACGTGGGCT 
GATGTGGAGA ATCTCAACAG CCATAATGAA GCTGAGCTCC GGCGTCAGTT TGAAGAGAGA 
CAGCAGGAGA CGGAGCATGT TTACGAGCTC CTGGAGAATA AGATCCAGCT TCTGCAGGAG 
GAATCCAGGC TAGCAAAGAA TGAGGCTGCA CGGATGGCAG CTCTGGTGGA AGCTGAGAAA 
GAATGTAACC TGGAGCTATC AGAGAAACTG AAGGGAGTTG CCAATGACAG GGAAGAGGTA 
CCAATGGACC AGGTCAATCC TGACCAATAC ACTGAGGCCC TGGCCCAGCG GGACAAAAGA 
ATTGAAGAAC TTCATCAAAG CCTGGCTTCC CAGGAGAAGC TTGTAGAACA GCTGTCTCAA 
GAGAAACAAC AACTGCTCCA TCTGTTGGAG GAACCAATGA GCATGGAAAT ACAGCCTGTG 
ACTGAAGAGT TACTCAAGCA ACAAAAGCTG GATTCAGATG AGACCATTAT AACTCAGCAG 
TCTGTATCTG ATTCCCACTT GGCTGAACTC CAGGAAAAAA TCCAGCAAAT GGAGGCCACC 
AACAAAATTC TTCAAGAGAA ACTGAATGAA ATGAGCTGTG AGCTAAAGTC TGCTCAGGAG 
TCATCTCAGA AGCAAGATGG TACAATTCAA AGCCTCAAGG AAACTCTGAA AAGTAGGGAA 
AATGAGACCG AGGAGTTGTA CCAGGTGATT GAAGGTCAAA ATGACACAAT GGCAAAGCTT 
CGAGAAATGC TACACCAAAG CCAGCTTGGA CAACTTCATG TATTTGAGGG TCACATAGGA 
CAGGAGAGCT CACAGGGTAC TTCCCCAGCT CAGCAACAGA TGGCTCTGCT TGATCTTCAG 
AGTGGTTTGT TCTACAGCCA GCTTGAGATT CAGAAACTCC AGAGGTTGGT ACGTCAAAAA 
GAACGCCAAC TGGCTGATGC CAAACGATGT GTCCAATTTG TAGAGGCTGC AGCCCATGAA 
AGAGAACAGC AGAAAGAGGC ATCTTGGAAA CATAATCAGG AATTACGGAA AGCCTTGCAG 
CAGCTCCAAG GAGAATTAGA GAGTAAGAGC CAACAGCTTC GTACCCTGGA GACTGAAAAA 
TGTAATGAGA TCAGAGCCCA TGAGCAACAC ATTCAACACC TAAACCATAG TCTGAGCCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ATCGAGATAA GTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTCGGC AGCGAATACA AGACCGAGAT 
GTTGCTTTGG AGCGGGCTAT AGATGAAAAG TTCTCCGCCC TGGAAGAGAA AGAAAAAGAA 
CTGCGTCAAT TTCATCTTGC TGTGCGAGAG CGTGATCATG ACTTAGAGAG ATTGCGTGCT 
ATTCTCTCCT CTAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GGCCAAAGGC 
CTAGAAGTAG AGCAGTTATC AGCTACCTGT CAAAACCTCC AGTGGCTGAA AGAAGAAAAG 
GAAACCAAAT TTAGCCATTG GCAGAAGGAA CAAGAAAGTA TCATTCAGCA GTTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTGAGTG CCACCTTGTT CTGTAAGCTT 
GGACCTGGGC AGAGTGAGGT AGCAGAAGAG CTGTGCCAGC GCCTACAGCG AAAGGAAAGG 
ATGCTACAGG AACTGCTAAC CGATCGAAAC AAACAAGCAG TGGAACACGA AATAGAGATT 
CAAGGCCTGC TTCAGTCTAT GAGCACCAGG GAGCAGGAGA GCCAAGCCGC TGCAGGGAAG 
ATGGTGCAAG CCCTTATGGA AAGAAATTCA GAACTACAGG CCCTGCGCCA GTATTTAGGA 
GGTAAAGACT TCATGGTTTC CCAAGCACTC ATCTCTAACC AACCCACAGA AGTCACCTCC 
AACAGCCCTC GTCTTGGAGA GCAGACTGAT CAAGGTTCAG TGCATATACC CTCCAAAGAT 
GATAGCATTT CCCTGACTGC CAAAGGGGAT ACCAGTATAC CCAGGTCCTC ATTAGGAGAC 
CAGGATACAG TTGCAGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA GCTTGAGCTC 
ATGGCTAAGA AAGAACGAGA AAGTCGGATG GAACTTTCTG CACTACAGTC CATGGTGGCC 
GTGCAAGAGG AAGAGCTGCA GGTGCAGGCT GCTGACATGG AATCCCTGAC CAGGAATATA 
CAGATTAAAG AAGACCTCAT AAAGGACCTG CAGATGCAAC TGGTTGATCC TGAAGATATA 
CCAGCTATGG AACGCCTGAC CCAAGAGGTC TTACTTCTTC GGGAAAAAGT TGCTTCAGTA 
GAATCACAGG GTCAGGAAAC GTTAGGAAAT CGAAGACAAC AATTGCTGCT AATGCTCGAA 
GGGCTGGTGG ATGAACGGAG TCGGCTCAAT GAAGCTTTAC AAGCAGAGAG ACAGCTGTAC 
AGCAGCCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGT GGTCGGCTAG AAGAAGTTCT TGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCTGCCATTG GAGGTGCAGC TGCAGGGGAA 
GATACAGAAG ATGCTAGCAC TGAGTTCACG GACAGCATCG AGGAGGAGGC TGCACACCAT 
AATCACCAGC AACTCATCAA GGTGGCTTTG GAGAAAAGTC TGGCAACTGT GGAGACTCAG 
AACGTATGTC TCCCCCCTCC TTCCTCAGCA GGAGGGGACA ATAACAGGGG TCTTCAGGAG 
GAGATGCTCC ACCTGAGGGC TGAGATCCAC CAGCACTTAG AGGAGAAGAG GAGAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATCGAG GAAGACAAGG AGAAGGGCGA GGTGACAGTG 
GAAACTGTGG CAGCCAAAGG AGGTCTGAAT GAAAATAGCC TTCAGGCTGA GTTCAGAAAG 
CTCCAGGGAA AACTGAAGCA TGCCCACAAC ATTATCAACC TCCTCAAAGA GCAGCTGGTG 
CTGAGCAGCA AGGATGGGAA TAGTAAACTT AGTCCAGAGC TCCTAGTGCG CCTGGACCGG 
GAGATCGACA GAATGAACAC AGAGCTGGTC TGTTCTCCGG AGAAGCCCCA GTGCCAGGAA 
GAAGAGCATA TGACCACAAG GCCCTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTACC 
CTCTCAGTGG ATGGCCACCA ACTGGATAAC CGGCCCCAGG CCCAAGACCC TGGGCCTCAG 
TCAGAATTTA GCCTTCCTGG ATCCACCAAG CACCTGCGCT CCCAGCTGGC CCAATGCAGA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCGG AAGCCACAGT CTTTGCACAG 
GCCAACCAAC TGGAGAAATA CCGAGTCATG CTTAGTGAAT CCTTGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGATCTG GGCTATGAAA CCTGTGGCCG AAGTGAGAAT 
GAGGCTGAGC GGGAGGAGAG CACCAGTCCC GAGTGTGAGG AGCCTGACAG CCTCAGGGAA 
ACAGTCCTGA TGGTGGGGCA CCTGGGCTCA CTGCTGGCCA GCCCTCCAGG GAAGAAGCCC 
TTGGAAAGCC ATCGGGGGAA GCAGGAGGAG TTCCAGGCAT ATGGAAAGTC AGAAGACATC 
TCTGTCCTAC ATAAGGACAT CAGAGATCTG AAGGCCCAGC TGCAGAATGC CAACAAGATC 
ATTCAGAATC TTAAGAGCCG GGTCCGGTCC CTCTCAGTTA CAAGTGATTA TTCATCTAGT 
CTGGAGAGAC CAAGAAAGCT GAAGGCCATT GGCACCCTTG AGGGATCCTC ACCTCACAGT 
GTCACTGACG AGGATGAGGG GTGGCTGTCT GATGGCACTG GGGCTTTCTG CACCCCAGGG 
CTTCAGGCTA AAAAGGATCT GGAGAGTCTG ATCCAGAGAG TGTCTCAGCT GGAGGCCCAG 
CTCCCGAAAA CTGGAGTGGA AGGGAAGCTA GCAGAGGAGC TGAGATCAGC CTCATGGCCT 
GGGAAATATG ATTCCCTAAT TCAGGATCAG GCCCGAGAGC TGTCGTACCT ACGCCAGAAG 
ATACGAGAAG GGAGAGGTAT CTGTTATCTT CTCACCCAGC ATGCAAAAGA TACAGTAAAA 
TCTTTTGAGG ACCTTCTTAG GAGCAATGAC ATTGACTACT ACCTGGGCCA GAGCTTCCGT 
GAGCAACTTG CCCAGGGAAG CCAGCTGACA GAGAGACTCA CCAGTAAACT CAGCACCAAG 
GATCATAAAA GTGAGAAAGA TCAAGCTGGA CTTGAACCAC TGGCCCTCAG GCTCAGCAGG 
GAGCTGCAGG AGAAGGAGAA AGTGATTGAA GTCCTGCAGG CCAAGCTAGA TGCTCGGTCT 
CTCACACCCT CCAGCAGCCA TGCATTGTCA GACTCCCACC GCTCTCCCAG CAGCTCGTCC 
TTCCTGTCTG ATGAGCTGGA AGCCTGCTCT GACATGGATG TAGCCAGCGA GTACACACAT 
TATGAAGAGA AGAAGGCTCC ACCTGGTCAC TCAGATTCCA TCCATCATTT GAGTCATTCT 
GCTGTACTGT CTTCTAAACC ATCAGCAACC AGTGTACCTC GGGGGGTTAA GGCCGAACCC 
AGCAGCAACC CCATCAGCTT GCCAGCTCCC CAGAACCCCC CCCAAGAGGC CAGCCAGGCC 
CATCCAGGCC TTCATTTTCC CTCCATATCC ACACTGGTTA GCCTTCCCCA GGCACCACTG 
CCCTCAGCTC CACCCAGCTT CCTGCCTTTC AGCTCCACTG GCCCTCCCCT CCTTGGCTGC 
TGTGAGACAC CTATGGTCTC CTTGGCAGAG GCTCAGCAGG AGCTGCAGAT GCTGCAGAAA 
CAATTGGGAG AAAGTGTCAG CACCGTCCAT CCTGCTTCCC CAGCTGCATT GCCAAGCAAC 
CATTTAGAAG CCAACTCTTC CCAATATCTC AACCCTGCCC AGCCCCACTC TCCTCCAACT 
GGCAGCACAG AATTGGGCAG AATCCTGGAG CCTGGCTACC TGGGTAGCGG CAGCCAGTGG 
GATGTGATGA GGCCTCAGAA GGGGAGTGTA TCAGGCGACT TATCCTCAGG ATCCTCTGTG 
TGTCAGCTTA ACTCCAAACC CACAGGGGCT GACCTGCTAG AAGAACATCT TGGTGAAATC 
CGGAACCTGC GCCAGCGCCT AGAGGAGTCC ATCTGCATCA ATGACCGCCT GCGGGAACAG 
CTGGAACACC GGCTCAGCTG CACAGCACGT GGCAATGGAT CCACCTCTAA TTTCTACAGT 
CCTGGCCTGG AACCCACACC TCAGCTCTAC AATGAGAACA GAGTCCTTAA GGAAGAAAAC 
CGGAGCCTTC AGGCTCAGCT GAGTCATGTT TCTAGAGAGC GCTCCCAGGA GACAGAATGC 
CTGAGGGAGG CTCTACTGTC CTCTCAATCC CGCCTTCAAG AGCTGGAAAT GGAGCTAGAG 
CACCAAAAGG AGGAACGGCA GCAGCTTTTG GAAGACTTGA AGGAGAAGCA GCAGGAGATC 
TTGCACTTCC AGGAGGAGCG CCTGTCCCTC CAGGAGAAAG ACTCTAGGCT GCAGCGTAAG 
CTGGCCCTAC TGCAGCAACA GTGTGAAGAG AAACAGCAGC TCTTCCAGTC CCTCCAGTCA 
GAGCTACAGA TCTATGAGGC GCTTTATGGC AGTTCCAAGA AGGGTCTGAA AGCTTCCACT 
TGGGATGCCT ATCACCAGGT CCCTTTGAGC AGTGACTTGA GCCACCTGGT GGCAGAGATC 
CGAGCTTTGC GAGGGCAGCT GGAGCAAAGC ATACAGGTGA ATAACTGTCT GCGACTGCAG 
CTGGAGCAGC AGCTGGATGG CGGAAGGAGC AAAACCAGCC TCAGTCCCTC TTCCACCAGC 
CAGGAGGACC CTGGAGACAA GCAAGACTCA ATTGCTTCCC CTCCAGTCCG AGATGTTGGC 
ATGAATTCTC CAGCTCTCAT GTTCCCTAGC TCTTCTCCTG CTGCTCCAGG CTCAGAGACA 
GCTATTTTCA ATAGGATAAA TGAGCTGAAT TTGGATGCTT TTCCAGTAAT GAAGAACTCT 
CCCAAGCTGG AGGGCGAAGC TATCGATGGC TCCTTTGCCA ATAAGCACGG CCGCCATGTG 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGACAGCAGA TTGGTGAGGG CAAAGTGCTG 
GTCAAAAAGA TAACAGCACT TGTGAGATCA GCCTACACCC TCCCTGGCCT TGAAACTCAG 
GCGACAGAGG TGCCAGGTGG CAAAGGCATC CATGAACTTG GGAGCAGCAC CCAAGCCCTG 
CAGCACGTGC TGGAGGAGTC GGCCTCCCTC CTCAATATGT TCTGGCGAGC GGCCCTGCCA 
AGCTCCAGCC CTGCCCTGTC TGGCAACATG GGACAGGCCA TGAAAAAGGA ACTTCTGGAA 
CTGAGAACCA AACTGACCAA ACAGGAGACC CTCCTTCAGT GCACAGCTGA GCGTCTAAAG 
ACCACCCACC AGCAGAAGGA GAGCATGGAG CAGTTCATCT TCAGCCATTT GACCAGGACA 
CATGATGTTT TGAAGAAGGC AAGGACTAAT TTGGAGGCCA AGTCAGGAAT CCGAGGAAAG 
CAAAAGAACA CTTACAAGAT GGCCTCTGTA AAGCCTTATT CCTGCCCCAG CAAAGGAGCC 
ACGCAAGAAG AGGAGCCCCC AGAGGTCCTT CAAACAGCAG GAAGGATGGG CCTGCCCCCC 
TTTTGTGCAG GTCCCAATCT GCTGAGGAGG CTTGGACCCC ACTCCTCCGC ACTGGCTGGA 
GGGTCCAGGA GGAGTCAGGC ATGGCCCAGT GGACGAGGGG GAAAGGAAGG CAGCCTTTCC 
TGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976074.1
CDS	350..7798
Translation	MEQTWTRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120599.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120599.2

ORF Insert Sequence:

ATGGAGCAGA CGTGGACCAG AGATTACTTT GCAGAGGATG AAGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACTAAAGATC GAGGCCCTCC AGTGCAATCA 
CAGATCTGGC GAAGTGGTGA AAAGGCCTCC TTTGGACAGA CGCCTTCCTT GAGAGCATTT 
GAAAAACCCC CTCAGGTGCA GACCCAGGCT CTTAGAGACT TTGAGAAGCA TCTCAATGAC 
CTGAAGAAGG AGAACTTCAG CCTCAAGCTG CGCATCTATT TCCTGGAGGA ACGCATGCAG 
CAGAAGTATG AGGCCAGCCG GGAGGACATC TACAAGCGGA ACATCGAGCT GAAGGTTGAA 
GTAGAGAGCC TGAAACGGGA ACTCCAGGAC AAGAAACAGC ATCTAGATAA AACGTGGGCT 
GATGTGGAGA ATCTCAACAG CCATAATGAA GCTGAGCTCC GGCGTCAGTT TGAAGAGAGA 
CAGCAGGAGA CGGAGCATGT TTACGAGCTC CTGGAGAATA AGATCCAGCT TCTGCAGGAG 
GAATCCAGGC TAGCAAAGAA TGAGGCTGCA CGGATGGCAG CTCTGGTGGA AGCTGAGAAA 
GAATGTAACC TGGAGCTATC AGAGAAACTG AAGGGAGTTG CCAATGACAG GGAAGAGGTA 
CCAATGGACC AGGTCAATCC TGACCAATAC ACTGAGGCCC TGGCCCAGCG GGACAAAAGA 
ATTGAAGAAC TTCATCAAAG CCTGGCTTCC CAGGAGAAGC TTGTAGAACA GCTGTCTCAA 
GAGAAACAAC AACTGCTCCA TCTGTTGGAG GAACCAATGA GCATGGAAAT ACAGCCTGTG 
ACTGAAGAGT TACTCAAGCA ACAAAAGCTG GATTCAGATG AGACCATTAT AACTCAGCAG 
TCTGTATCTG ATTCCCACTT GGCTGAACTC CAGGAAAAAA TCCAGCAAAT GGAGGCCACC 
AACAAAATTC TTCAAGAGAA ACTGAATGAA ATGAGCTGTG AGCTAAAGTC TGCTCAGGAG 
TCATCTCAGA AGCAAGATGG TACAATTCAA AGCCTCAAGG AAACTCTGAA AAGTAGGGAA 
AATGAGACCG AGGAGTTGTA CCAGGTGATT GAAGGTCAAA ATGACACAAT GGCAAAGCTT 
CGAGAAATGC TACACCAAAG CCAGCTTGGA CAACTTCATG TATTTGAGGG TCACATAGGA 
CAGGAGAGCT CACAGGGTAC TTCCCCAGCT CAGCAACAGA TGGCTCTGCT TGATCTTCAG 
AGTGGTTTGT TCTACAGCCA GCTTGAGATT CAGAAACTCC AGAGGTTGGT ACGTCAAAAA 
GAACGCCAAC TGGCTGATGC CAAACGATGT GTCCAATTTG TAGAGGCTGC AGCCCATGAA 
AGAGAACAGC AGAAAGAGGC ATCTTGGAAA CATAATCAGG AATTACGGAA AGCCTTGCAG 
CAGCTCCAAG GAGAATTAGA GAGTAAGAGC CAACAGCTTC GTACCCTGGA GACTGAAAAA 
TGTAATGAGA TCAGAGCCCA TGAGCAACAC ATTCAACACC TAAACCATAG TCTGAGCCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ATCGAGATAA GTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTCGGC AGCGAATACA AGACCGAGAT 
GTTGCTTTGG AGCGGGCTAT AGATGAAAAG TTCTCCGCCC TGGAAGAGAA AGAAAAAGAA 
CTGCGTCAAT TTCATCTTGC TGTGCGAGAG CGTGATCATG ACTTAGAGAG ATTGCGTGCT 
ATTCTCTCCT CTAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GGCCAAAGGC 
CTAGAAGTAG AGCAGTTATC AGCTACCTGT CAAAACCTCC AGTGGCTGAA AGAAGAAAAG 
GAAACCAAAT TTAGCCATTG GCAGAAGGAA CAAGAAAGTA TCATTCAGCA GTTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTGAGTG CCACCTTGTT CTGTAAGCTT 
GGACCTGGGC AGAGTGAGGT AGCAGAAGAG CTGTGCCAGC GCCTACAGCG AAAGGAAAGG 
ATGCTACAGG AACTGCTAAC CGATCGAAAC AAACAAGCAG TGGAACACGA AATAGAGATT 
CAAGGCCTGC TTCAGTCTAT GAGCACCAGG GAGCAGGAGA GCCAAGCCGC TGCAGGGAAG 
ATGGTGCAAG CCCTTATGGA AAGAAATTCA GAACTACAGG CCCTGCGCCA GTATTTAGGA 
GGTAAAGACT TCATGGTTTC CCAAGCACTC ATCTCTAACC AACCCACAGA AGTCACCTCC 
AACAGCCCTC GTCTTGGAGA GCAGACTGAT CAAGGTTCAG TGCATATACC CTCCAAAGAT 
GATAGCATTT CCCTGACTGC CAAAGGGGAT ACCAGTATAC CCAGGTCCTC ATTAGGAGAC 
CAGGATACAG TTGCAGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA GCTTGAGCTC 
ATGGCTAAGA AAGAACGAGA AAGTCGGATG GAACTTTCTG CACTACAGTC CATGGTGGCC 
GTGCAAGAGG AAGAGCTGCA GGTGCAGGCT GCTGACATGG AATCCCTGAC CAGGAATATA 
CAGATTAAAG AAGACCTCAT AAAGGACCTG CAGATGCAAC TGGTTGATCC TGAAGATATA 
CCAGCTATGG AACGCCTGAC CCAAGAGGTC TTACTTCTTC GGGAAAAAGT TGCTTCAGTA 
GAATCACAGG GTCAGGAAAC GTTAGGAAAT CGAAGACAAC AATTGCTGCT AATGCTCGAA 
GGGCTGGTGG ATGAACGGAG TCGGCTCAAT GAAGCTTTAC AAGCAGAGAG ACAGCTGTAC 
AGCAGCCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGT GGTCGGCTAG AAGAAGTTCT TGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCTGCCATTG GAGGTGCAGC TGCAGGGGAA 
GATACAGAAG ATGCTAGCAC TGAGTTCACG GACAGCATCG AGGAGGAGGC TGCACACCAT 
AATCACCAGC AACTCATCAA GGTGGCTTTG GAGAAAAGTC TGGCAACTGT GGAGACTCAG 
AACGTATGTC TCCCCCCTCC TTCCTCAGCA GGAGGGGACA ATAACAGGGG TCTTCAGGAG 
GAGATGCTCC ACCTGAGGGC TGAGATCCAC CAGCACTTAG AGGAGAAGAG GAGAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATCGAG GAAGACAAGG AGAAGGGCGA GGTGACAGTG 
GAAACTGTGG CAGCCAAAGG AGGTCTGAAT GAAAATAGCC TTCAGGCTGA GTTCAGAAAG 
CTCCAGGGAA AACTGAAGCA TGCCCACAAC ATTATCAACC TCCTCAAAGA GCAGCTGGTG 
CTGAGCAGCA AGGATGGGAA TAGTAAACTT AGTCCAGAGC TCCTAGTGCG CCTGGACCGG 
GAGATCGACA GAATGAACAC AGAGCTGGTC TGTTCTCCGG AGAAGCCCCA GTGCCAGGAA 
GAAGAGCATA TGACCACAAG GCCCTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTACC 
CTCTCAGTGG ATGGCCACCA ACTGGATAAC CGGCCCCAGG CCCAAGACCC TGGGCCTCAG 
TCAGAATTTA GCCTTCCTGG ATCCACCAAG CACCTGCGCT CCCAGCTGGC CCAATGCAGA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCGG AAGCCACAGT CTTTGCACAG 
GCCAACCAAC TGGAGAAATA CCGAGTCATG CTTAGTGAAT CCTTGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGATCTG GGCTATGAAA CCTGTGGCCG AAGTGAGAAT 
GAGGCTGAGC GGGAGGAGAG CACCAGTCCC GAGTGTGAGG AGCCTGACAG CCTCAGGGAA 
ACAGTCCTGA TGGTGGGGCA CCTGGGCTCA CTGCTGGCCA GCCCTCCAGG GAAGAAGCCC 
TTGGAAAGCC ATCGGGGGAA GCAGGAGGAG TTCCAGGCAT ATGGAAAGTC AGAAGACATC 
TCTGTCCTAC ATAAGGACAT CAGAGATCTG AAGGCCCAGC TGCAGAATGC CAACAAGATC 
ATTCAGAATC TTAAGAGCCG GGTCCGGTCC CTCTCAGTTA CAAGTGATTA TTCATCTAGT 
CTGGAGAGAC CAAGAAAGCT GAAGGCCATT GGCACCCTTG AGGGATCCTC ACCTCACAGT 
GTCACTGACG AGGATGAGGG GTGGCTGTCT GATGGCACTG GGGCTTTCTG CACCCCAGGG 
CTTCAGGCTA AAAAGGATCT GGAGAGTCTG ATCCAGAGAG TGTCTCAGCT GGAGGCCCAG 
CTCCCGAAAA CTGGAGTGGA AGGGAAGCTA GCAGAGGAGC TGAGATCAGC CTCATGGCCT 
GGGAAATATG ATTCCCTAAT TCAGGATCAG GCCCGAGAGC TGTCGTACCT ACGCCAGAAG 
ATACGAGAAG GGAGAGGTAT CTGTTATCTT CTCACCCAGC ATGCAAAAGA TACAGTAAAA 
TCTTTTGAGG ACCTTCTTAG GAGCAATGAC ATTGACTACT ACCTGGGCCA GAGCTTCCGT 
GAGCAACTTG CCCAGGGAAG CCAGCTGACA GAGAGACTCA CCAGTAAACT CAGCACCAAG 
GATCATAAAA GTGAGAAAGA TCAAGCTGGA CTTGAACCAC TGGCCCTCAG GCTCAGCAGG 
GAGCTGCAGG AGAAGGAGAA AGTGATTGAA GTCCTGCAGG CCAAGCTAGA TGCTCGGTCT 
CTCACACCCT CCAGCAGCCA TGCATTGTCA GACTCCCACC GCTCTCCCAG CAGCTCGTCC 
TTCCTGTCTG ATGAGCTGGA AGCCTGCTCT GACATGGATG TAGCCAGCGA GTACACACAT 
TATGAAGAGA AGAAGGCTCC ACCTGGTCAC TCAGATTCCA TCCATCATTT GAGTCATTCT 
GCTGTACTGT CTTCTAAACC ATCAGCAACC AGTGTACCTC GGGGGGTTAA GGCCGAACCC 
AGCAGCAACC CCATCAGCTT GCCAGCTCCC CAGAACCCCC CCCAAGAGGC CAGCCAGGCC 
CATCCAGGCC TTCATTTTCC CTCCATATCC ACACTGGTTA GCCTTCCCCA GGCACCACTG 
CCCTCAGCTC CACCCAGCTT CCTGCCTTTC AGCTCCACTG GCCCTCCCCT CCTTGGCTGC 
TGTGAGACAC CTATGGTCTC CTTGGCAGAG GCTCAGCAGG AGCTGCAGAT GCTGCAGAAA 
CAATTGGGAG AAAGTGTCAG CACCGTCCAT CCTGCTTCCC CAGCTGCATT GCCAAGCAAC 
CATTTAGAAG CCAACTCTTC CCAATATCTC AACCCTGCCC AGCCCCACTC TCCTCCAACT 
GGCAGCACAG AATTGGGCAG AATCCTGGAG CCTGGCTACC TGGGTAGCGG CAGCCAGTGG 
GATGTGATGA GGCCTCAGAA GGGGAGTGTA TCAGGCGACT TATCCTCAGG ATCCTCTGTG 
TGTCAGCTTA ACTCCAAACC CACAGGGGCT GACCTGCTAG AAGAACATCT TGGTGAAATC 
CGGAACCTGC GCCAGCGCCT AGAGGAGTCC ATCTGCATCA ATGACCGCCT GCGGGAACAG 
CTGGAACACC GGCTCAGCTG CACAGCACGT GGCAATGGAT CCACCTCTAA TTTCTACAGT 
CCTGGCCTGG AACCCACACC TCAGCTCTAC AATGAGAACA GAGTCCTTAA GGAAGAAAAC 
CGGAGCCTTC AGGCTCAGCT GAGTCATGTT TCTAGAGAGC GCTCCCAGGA GACAGAATGC 
CTGAGGGAGG CTCTACTGTC CTCTCAATCC CGCCTTCAAG AGCTGGAAAT GGAGCTAGAG 
CACCAAAAGG AGGAACGGCA GCAGCTTTTG GAAGACTTGA AGGAGAAGCA GCAGGAGATC 
TTGCACTTCC AGGAGGAGCG CCTGTCCCTC CAGGAGAAAG ACTCTAGGCT GCAGCGTAAG 
CTGGCCCTAC TGCAGCAACA GTGTGAAGAG AAACAGCAGC TCTTCCAGTC CCTCCAGTCA 
GAGCTACAGA TCTATGAGGC GCTTTATGGC AGTTCCAAGA AGGGTCTGAA AGCTTCCACT 
TGGGATGCCT ATCACCAGGT CCCTTTGAGC AGTGACTTGA GCCACCTGGT GGCAGAGATC 
CGAGCTTTGC GAGGGCAGCT GGAGCAAAGC ATACAGGTGA ATAACTGTCT GCGACTGCAG 
CTGGAGCAGC AGCTGGATGG CGGAAGGAGC AAAACCAGCC TCAGTCCCTC TTCCACCAGC 
CAGGAGGACC CTGGAGACAA GCAAGACTCA ATTGCTTCCC CTCCAGTCCG AGATGTTGGC 
ATGAATTCTC CAGCTCTCAT GTTCCCTAGC TCTTCTCCTG CTGCTCCAGG CTCAGAGACA 
GCTATTTTCA ATAGGATAAA TGAGCTGAAT TTGGATGCTT TTCCAGTAAT GAAGAACTCT 
CCCAAGCTGG AGGGCGAAGC TATCGATGGC TCCTTTGCCA ATAAGCACGG CCGCCATGTG 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGACAGCAGA TTGGTGAGGG CAAAGTGCTG 
GTCAAAAAGA TAACAGCACT TGTGAGATCA GCCTACACCC TCCCTGGCCT TGAAACTCAG 
GCGACAGAGG TGCCAGGTGG CAAAGGCATC CATGAACTTG GGAGCAGCAC CCAAGCCCTG 
CAGCACGTGC TGGAGGAGTC GGCCTCCCTC CTCAATATGT TCTGGCGAGC GGCCCTGCCA 
AGCTCCAGCC CTGCCCTGTC TGGCAACATG GGACAGGCCA TGAAAAAGGA ACTTCTGGAA 
CTGAGAACCA AACTGACCAA ACAGGAGACC CTCCTTCAGT GCACAGCTGA GCGTCTAAAG 
ACCACCCACC AGCAGAAGGA GAGCATGGAG CAGTTCATCT TCAGCCATTT GACCAGGACA 
CATGATGTTT TGAAGAAGGC AAGGACTAAT TTGGAGGCCA AGTCAGGAAT CCGAGGAAAG 
CAAAAGAACA CTTACAAGAT GGCCTCTGTA AAGCCTTATT CCTGCCCCAG CAAAGGAGCC 
ACGCAAGAAG AGGAGCCCCC AGAGGTCCTT CAAACAGCAG GAAGGATGGG CCTGCCCCCC 
TTTTGTGCAG GTCCCAATCT GCTGAGGAGG CTTGGACCCC ACTCCTCCGC ACTGGCTGGA 
GGGTCCAGGA GGAGTCAGGC ATGGCCCAGT GGACGAGGGG GAAAGGAAGG CAGCCTTTCC 
TGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120600.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976075.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X15, mRNA.

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CloneID	OCe40886
Clone ID Related Accession (Same CDS sequence)	XM_014120600.1
Accession Version	XM_014120600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CATCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA TTTCCTGGAG GAACGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGAGGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGG 
GAACTCCAGG ACAAGAAACA GCATCTAGAT AAAACGTGGG CTGATGTGGA GAATCTCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGTCAG TTTGAAGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAGG AGGAATCCAG GCTAGCAAAG 
AATGAGGCTG CACGGATGGC AGCTCTGGTG GAAGCTGAGA AAGAATGTAA CCTGGAGCTA 
TCAGAGAAAC TGAAGGGAGT TGCCAATGAC AGGGAAGAGG TACCAATGGA CCAGGTCAAT 
CCTGACCAAT ACACTGAGGC CCTGGCCCAG CGGGACAAAA GAATTGAAGA ACTTCATCAA 
AGCCTGGCTT CCCAGGAGAA GCTTGTAGAA CAGCTGTCTC AAGAGAAACA ACAACTGCTC 
CATCTGTTGG AGGAACCAAT GAGCATGGAA ATACAGCCTG TGACTGAAGA GTTACTCAAG 
CAACAAAAGC TGGATTCAGA TGAGACCATT ATAACTCAGC AGTCTGTATC TGATTCCCAC 
TTGGCTGAAC TCCAGGAAAA AATCCAGCAA ATGGAGGCCA CCAACAAAAT TCTTCAAGAG 
AAACTGAATG AAATGAGCTG TGAGCTAAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GGTACAATTC AAAGCCTCAA GGAAACTCTG AAAAGTAGGG AAAATGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAATGACACA ATGGCAAAGC TTCGAGAAAT GCTACACCAA 
AGCCAGCTTG GACAACTTCA TGTATTTGAG GGTCACATAG GACAGGAGAG CTCACAGGGT 
ACTTCCCCAG CTCAGCAACA GATGGCTCTG CTTGATCTTC AGAGTGGTTT GTTCTACAGC 
CAGCTTGAGA TTCAGAAACT CCAGAGGTTG GTACGTCAAA AAGAACGCCA ACTGGCTGAT 
GCCAAACGAT GTGTCCAATT TGTAGAGGCT GCAGCCCATG AAAGAGAACA GCAGAAAGAG 
GCATCTTGGA AACATAATCA GGAATTACGG AAAGCCTTGC AGCAGCTCCA AGGAGAATTA 
GAGAGTAAGA GCCAACAGCT TCGTACCCTG GAGACTGAAA AATGTAATGA GATCAGAGCC 
CATGAGCAAC ACATTCAACA CCTAAACCAT AGTCTGAGCC ACAAAGAGCA GCTGCTTCAG 
GAATTTCGGG AGCTCCTGCA GTATCGAGAT AAGTCAGACA AAACCCTTGA AGCAAATGAA 
ATGTTGCTTG AGAAACTTCG GCAGCGAATA CAAGACCGAG ATGTTGCTTT GGAGCGGGCT 
ATAGATGAAA AGTTCTCCGC CCTGGAAGAG AAAGAAAAAG AACTGCGTCA ATTTCATCTT 
GCTGTGCGAG AGCGTGATCA TGACTTAGAG AGATTGCGTG CTATTCTCTC CTCTAATGAA 
GCTACCATGC AGAGTATGGA GAGTCTCCTG AGGGCCAAAG GCCTAGAAGT AGAGCAGTTA 
TCAGCTACCT GTCAAAACCT CCAGTGGCTG AAAGAAGAAA AGGAAACCAA ATTTAGCCAT 
TGGCAGAAGG AACAAGAAAG TATCATTCAG CAGTTACAGA CATCTCTGCA TGACAGGAAC 
AAAGAAGTGG AGGATCTGAG TGCCACCTTG TTCTGTAAGC TTGGACCTGG GCAGAGTGAG 
GTAGCAGAAG AGCTGTGCCA GCGCCTACAG CGAAAGGAAA GGATGCTACA GGAACTGCTA 
ACCGATCGAA ACAAACAAGC AGTGGAACAC GAAATAGAGA TTCAAGGCCT GCTTCAGTCT 
ATGAGCACCA GGGAGCAGGA GAGCCAAGCC GCTGCAGGGA AGATGGTGCA AGCCCTTATG 
GAAAGAAATT CAGAACTACA GGCCCTGCGC CAGTATTTAG GAGGTAAAGA CTTCATGGTT 
TCCCAAGCAC TCATCTCTAA CCAACCCACA GAAGTCACCT CCAACAGCCC TCGTCTTGGA 
GAGCAGACTG ATCAAGGTTC AGTGCATATA CCCTCCAAAG ATGATAGCAT TTCCCTGACT 
GCCAAAGGGG ATACCAGTAT ACCCAGGTCC TCATTAGGAG ACCAGGATAC AGTTGCAGGG 
CTGGAAAAAG AACTGAGTAA TGCCAAAGAG GAGCTTGAGC TCATGGCTAA GAAAGAACGA 
GAAAGTCGGA TGGAACTTTC TGCACTACAG TCCATGGTGG CCGTGCAAGA GGAAGAGCTG 
CAGGTGCAGG CTGCTGACAT GGAATCCCTG ACCAGGAATA TACAGATTAA AGAAGACCTC 
ATAAAGGACC TGCAGATGCA ACTGGTTGAT CCTGAAGATA TACCAGCTAT GGAACGCCTG 
ACCCAAGAGG TCTTACTTCT TCGGGAAAAA GTTGCTTCAG TAGAATCACA GGGTCAGGAA 
ACGTTAGGAA ATCGAAGACA ACAATTGCTG CTAATGCTCG AAGGGCTGGT GGATGAACGG 
AGTCGGCTCA ATGAAGCTTT ACAAGCAGAG AGACAGCTGT ACAGCAGCCT GGTGAAGTTC 
CATGCCCATC CAGAGAGCTC TGAGAGAGAC CGAACTCTGC AGGTGGAACT AGAAGGGGCC 
CAGGTGATAC GTGGTCGGCT AGAAGAAGTT CTTGGAAGAA GCCTGGAGCG CTTAAGCAGG 
CTGGAGACCC TGGCTGCCAT TGGAGGTGCA GCTGCAGGGG AAGATACAGA AGATGCTAGC 
ACTGAGTTCA CGGACAGCAT CGAGGAGGAG GCTGCACACC ATAATCACCA GCAACTCATC 
AAGGTGGCTT TGGAGAAAAG TCTGGCAACT GTGGAGACTC AGAACGTATG TCTCCCCCCT 
CCTTCCTCAG CAGGAGGGGA CAATAACAGG GGTCTTCAGG AGGAGATGCT CCACCTGAGG 
GCTGAGATCC ACCAGCACTT AGAGGAGAAG AGGAGAGCTG AGGGGGAACT GAAGGAGCTA 
AAGGCTCAAA TTGAGGAAGC AGGATTCTCC TCTGTGGCCC ACATCAGGAA CACCATGCTG 
AGCCTTTGCC TTGAGAATGC AGAGCTGAAA GAGCAGATGG GAGAAGCAAT GTCTGATGGA 
TGGGAGATCG AGGAAGACAA GGAGAAGGGC GAGGTGACAG TGGAAACTGT GGCAGCCAAA 
GGAGGTCTGA ATGAAAATAG CCTTCAGGCT GAGTTCAGAA AGCTCCAGGG AAAACTGAAG 
CATGCCCACA ACATTATCAA CCTCCTCAAA GAGCAGCTGG TGCTGAGCAG CAAGGATGGG 
AATAGTAAAC TTAGTCCAGA GCTCCTAGTG CGCCTGGACC GGGAGATCGA CAGAATGAAC 
ACAGAGCTGG TCTGTTCTCC GGAGAAGCCC CAGTGCCAGG AAGAAGAGCA TATGACCACA 
AGGCCCTGCC CCAGACCCCA GAGCCTTGAC CTTGGGGCTA CCCTCTCAGT GGATGGCCAC 
CAACTGGATA ACCGGCCCCA GGCCCAAGAC CCTGGGCCTC AGTCAGAATT TAGCCTTCCT 
GGATCCACCA AGCACCTGCG CTCCCAGCTG GCCCAATGCA GACAACGCTA TCAGGATCTC 
CAGGAGAAGC TGCTGATATC GGAAGCCACA GTCTTTGCAC AGGCCAACCA ACTGGAGAAA 
TACCGAGTCA TGCTTAGTGA ATCCTTGGTG AAGCAGGACA GCAAGCAGGT GCAGGTGGAC 
CTCCAGGATC TGGGCTATGA AACCTGTGGC CGAAGTGAGA ATGAGGCTGA GCGGGAGGAG 
AGCACCAGTC CCGAGTGTGA GGAGCCTGAC AGCCTCAGGG AAACAGTCCT GATGGTGGGG 
CACCTGGGCT CACTGCTGGC CAGCCCTCCA GGGAAGAAGC CCTTGGAAAG CCATCGGGGG 
AAGCAGGAGG AGTTCCAGGC ATATGGAAAG TCAGAAGACA TCTCTGTCCT ACATAAGGAC 
ATCAGAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGA TCATTCAGAA TCTTAAGAGC 
CGGGTCCGGT CCCTCTCAGT TACAAGTGAT TATTCATCTA GTCTGGAGAG ACCAAGAAAG 
CTGAAGGCCA TTGGCACCCT TGAGGGATCC TCACCTCACA GTGTCACTGA CGAGGATGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TGCACCCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG AGTGTCTCAG CTGGAGGCCC AGCTCCCGAA AACTGGAGTG 
GAAGGGAAGC TAGCAGAGGA GCTGAGATCA GCCTCATGGC CTGGGAAATA TGATTCCCTA 
ATTCAGGATC AGGCCCGAGA GCTGTCGTAC CTACGCCAGA AGATACGAGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCATGCAAAA GATACAGTAA AATCTTTTGA GGACCTTCTT 
AGGAGCAATG ACATTGACTA CTACCTGGGC CAGAGCTTCC GTGAGCAACT TGCCCAGGGA 
AGCCAGCTGA CAGAGAGACT CACCAGTAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTTGAACC ACTGGCCCTC AGGCTCAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATTG AAGTCCTGCA GGCCAAGCTA GATGCTCGGT CTCTCACACC CTCCAGCAGC 
CATGCATTGT CAGACTCCCA CCGCTCTCCC AGCAGCTCGT CCTTCCTGTC TGATGAGCTG 
GAAGCCTGCT CTGACATGGA TGTAGCCAGC GAGTACACAC ATTATGAAGA GAAGAAGGCT 
CCACCTGGTC ACTCAGATTC CATCCATCAT TTGAGTCATT CTGCTGTACT GTCTTCTAAA 
CCATCAGCAA CCAGTGTACC TCGGGGGGTT AAGGCCGAAC CCAGCAGCAA CCCCATCAGC 
TTGCCAGCTC CCCAGAACCC CCCCCAAGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTT 
CCCTCCATAT CCACACTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCCAC TGGCCCTCCC CTCCTTGGCT GCTGTGAGAC ACCTATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCAGA AACAATTGGG AGAAAGTGTC 
AGCACCGTCC ATCCTGCTTC CCCAGCTGCA TTGCCAAGCA ACCATTTAGA AGCCAACTCT 
TCCCAATATC TCAACCCTGC CCAGCCCCAC TCTCCTCCAA CTGGCAGCAC AGAATTGGGC 
AGAATCCTGG AGCCTGGCTA CCTGGGTAGC GGCAGCCAGT GGGATGTGAT GAGGCCTCAG 
AAGGGGAGTG TATCAGGCGA CTTATCCTCA GGATCCTCTG TGTGTCAGCT TAACTCCAAA 
CCCACAGGGG CTGACCTGCT AGAAGAACAT CTTGGTGAAA TCCGGAACCT GCGCCAGCGC 
CTAGAGGAGT CCATCTGCAT CAATGACCGC CTGCGGGAAC AGCTGGAACA CCGGCTCAGC 
TGCACAGCAC GTGGCAATGG ATCCACCTCT AATTTCTACA GTCCTGGCCT GGAACCCACA 
CCTCAGCTCT ACAATGAGAA CAGAGTCCTT AAGGAAGAAA ACCGGAGCCT TCAGGCTCAG 
CTGAGTCATG TTTCTAGAGA GCGCTCCCAG GAGACAGAAT GCCTGAGGGA GGCTCTACTG 
TCCTCTCAAT CCCGCCTTCA AGAGCTGGAA ATGGAGCTAG AGCACCAAAA GGAGGAACGG 
CAGCAGCTTT TGGAAGACTT GAAGGAGAAG CAGCAGGAGA TCTTGCACTT CCAGGAGGAG 
CGCCTGTCCC TCCAGGAGAA AGACTCTAGG CTGCAGCGTA AGCTGGCCCT ACTGCAGCAA 
CAGTGTGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCGCTTTATG GCAGTTCCAA GAAGGGTCTG AAAGCTTCCA CTTGGGATGC CTATCACCAG 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAAA GCATACAGGT GAATAACTGT CTGCGACTGC AGCTGGAGCA GCAGCTGGAT 
GGCGGAAGGA GCAAAACCAG CCTCAGTCCC TCTTCCACCA GCCAGGAGGA CCCTGGAGAC 
AAGCAAGACT CAATTGCTTC CCCTCCAGTC CGAGATGTTG GCATGAATTC TCCAGCTCTC 
ATGTTCCCTA GCTCTTCTCC TGCTGCTCCA GGCTCAGAGA CAGCTATTTT CAATAGGATA 
AATGAGCTGA ATTTGGATGC TTTTCCAGTA ATGAAGAACT CTCCCAAGCT GGAGGGCGAA 
GCTATCGATG GCTCCTTTGC CAATAAGCAC GGCCGCCATG TGATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGACAGCA GATTGGTGAG GGCAAAGTGC TGGTCAAAAA GATAACAGCA 
CTTGTGAGAT CAGCCTACAC CCTCCCTGGC CTTGAAACTC AGGCGACAGA GGTGCCAGGT 
GGCAAAGGCA TCCATGAACT TGGGAGCAGC ACCCAAGCCC TGCAGCACGT GCTGGAGGAG 
TCGGCCTCCC TCCTCAATAT GTTCTGGCGA GCGGCCCTGC CAAGCTCCAG CCCTGCCCTG 
TCTGGCAACA TGGGACAGGC CATGAAAAAG GAACTTCTGG AACTGAGAAC CAAACTGACC 
AAACAGGAGA CCCTCCTTCA GTGCACAGCT GAGCGTCTAA AGACCACCCA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAT TTGACCAGGA CACATGATGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGGC CAAGTCAGGA ATCCGAGGAA AGCAAAAGAA CACTTACAAG 
ATGGCCTCTG TAAAGCCTTA TTCCTGCCCC AGCAAAGGAG CCACGCAAGA AGAGGAGCCC 
CCAGAGGTCC TTCAAACAGC AGGAAGGATG GGCCTGCCCC CCTTTTGTGC AGGTCCCAAT 
CTGCTGAGGA GGCTTGGACC CCACTCCTCC GCACTGGCTG GAGGGTCCAG GAGGAGTCAG 
GCATGGCCCA GTGGACGAGG GGGAAAGGAA GGCAGCCTTT CCTGGGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976075.1
CDS	220..7470
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120600.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120600.1

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CATCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA TTTCCTGGAG GAACGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGAGGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGG 
GAACTCCAGG ACAAGAAACA GCATCTAGAT AAAACGTGGG CTGATGTGGA GAATCTCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGTCAG TTTGAAGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAGG AGGAATCCAG GCTAGCAAAG 
AATGAGGCTG CACGGATGGC AGCTCTGGTG GAAGCTGAGA AAGAATGTAA CCTGGAGCTA 
TCAGAGAAAC TGAAGGGAGT TGCCAATGAC AGGGAAGAGG TACCAATGGA CCAGGTCAAT 
CCTGACCAAT ACACTGAGGC CCTGGCCCAG CGGGACAAAA GAATTGAAGA ACTTCATCAA 
AGCCTGGCTT CCCAGGAGAA GCTTGTAGAA CAGCTGTCTC AAGAGAAACA ACAACTGCTC 
CATCTGTTGG AGGAACCAAT GAGCATGGAA ATACAGCCTG TGACTGAAGA GTTACTCAAG 
CAACAAAAGC TGGATTCAGA TGAGACCATT ATAACTCAGC AGTCTGTATC TGATTCCCAC 
TTGGCTGAAC TCCAGGAAAA AATCCAGCAA ATGGAGGCCA CCAACAAAAT TCTTCAAGAG 
AAACTGAATG AAATGAGCTG TGAGCTAAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GGTACAATTC AAAGCCTCAA GGAAACTCTG AAAAGTAGGG AAAATGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAATGACACA ATGGCAAAGC TTCGAGAAAT GCTACACCAA 
AGCCAGCTTG GACAACTTCA TGTATTTGAG GGTCACATAG GACAGGAGAG CTCACAGGGT 
ACTTCCCCAG CTCAGCAACA GATGGCTCTG CTTGATCTTC AGAGTGGTTT GTTCTACAGC 
CAGCTTGAGA TTCAGAAACT CCAGAGGTTG GTACGTCAAA AAGAACGCCA ACTGGCTGAT 
GCCAAACGAT GTGTCCAATT TGTAGAGGCT GCAGCCCATG AAAGAGAACA GCAGAAAGAG 
GCATCTTGGA AACATAATCA GGAATTACGG AAAGCCTTGC AGCAGCTCCA AGGAGAATTA 
GAGAGTAAGA GCCAACAGCT TCGTACCCTG GAGACTGAAA AATGTAATGA GATCAGAGCC 
CATGAGCAAC ACATTCAACA CCTAAACCAT AGTCTGAGCC ACAAAGAGCA GCTGCTTCAG 
GAATTTCGGG AGCTCCTGCA GTATCGAGAT AAGTCAGACA AAACCCTTGA AGCAAATGAA 
ATGTTGCTTG AGAAACTTCG GCAGCGAATA CAAGACCGAG ATGTTGCTTT GGAGCGGGCT 
ATAGATGAAA AGTTCTCCGC CCTGGAAGAG AAAGAAAAAG AACTGCGTCA ATTTCATCTT 
GCTGTGCGAG AGCGTGATCA TGACTTAGAG AGATTGCGTG CTATTCTCTC CTCTAATGAA 
GCTACCATGC AGAGTATGGA GAGTCTCCTG AGGGCCAAAG GCCTAGAAGT AGAGCAGTTA 
TCAGCTACCT GTCAAAACCT CCAGTGGCTG AAAGAAGAAA AGGAAACCAA ATTTAGCCAT 
TGGCAGAAGG AACAAGAAAG TATCATTCAG CAGTTACAGA CATCTCTGCA TGACAGGAAC 
AAAGAAGTGG AGGATCTGAG TGCCACCTTG TTCTGTAAGC TTGGACCTGG GCAGAGTGAG 
GTAGCAGAAG AGCTGTGCCA GCGCCTACAG CGAAAGGAAA GGATGCTACA GGAACTGCTA 
ACCGATCGAA ACAAACAAGC AGTGGAACAC GAAATAGAGA TTCAAGGCCT GCTTCAGTCT 
ATGAGCACCA GGGAGCAGGA GAGCCAAGCC GCTGCAGGGA AGATGGTGCA AGCCCTTATG 
GAAAGAAATT CAGAACTACA GGCCCTGCGC CAGTATTTAG GAGGTAAAGA CTTCATGGTT 
TCCCAAGCAC TCATCTCTAA CCAACCCACA GAAGTCACCT CCAACAGCCC TCGTCTTGGA 
GAGCAGACTG ATCAAGGTTC AGTGCATATA CCCTCCAAAG ATGATAGCAT TTCCCTGACT 
GCCAAAGGGG ATACCAGTAT ACCCAGGTCC TCATTAGGAG ACCAGGATAC AGTTGCAGGG 
CTGGAAAAAG AACTGAGTAA TGCCAAAGAG GAGCTTGAGC TCATGGCTAA GAAAGAACGA 
GAAAGTCGGA TGGAACTTTC TGCACTACAG TCCATGGTGG CCGTGCAAGA GGAAGAGCTG 
CAGGTGCAGG CTGCTGACAT GGAATCCCTG ACCAGGAATA TACAGATTAA AGAAGACCTC 
ATAAAGGACC TGCAGATGCA ACTGGTTGAT CCTGAAGATA TACCAGCTAT GGAACGCCTG 
ACCCAAGAGG TCTTACTTCT TCGGGAAAAA GTTGCTTCAG TAGAATCACA GGGTCAGGAA 
ACGTTAGGAA ATCGAAGACA ACAATTGCTG CTAATGCTCG AAGGGCTGGT GGATGAACGG 
AGTCGGCTCA ATGAAGCTTT ACAAGCAGAG AGACAGCTGT ACAGCAGCCT GGTGAAGTTC 
CATGCCCATC CAGAGAGCTC TGAGAGAGAC CGAACTCTGC AGGTGGAACT AGAAGGGGCC 
CAGGTGATAC GTGGTCGGCT AGAAGAAGTT CTTGGAAGAA GCCTGGAGCG CTTAAGCAGG 
CTGGAGACCC TGGCTGCCAT TGGAGGTGCA GCTGCAGGGG AAGATACAGA AGATGCTAGC 
ACTGAGTTCA CGGACAGCAT CGAGGAGGAG GCTGCACACC ATAATCACCA GCAACTCATC 
AAGGTGGCTT TGGAGAAAAG TCTGGCAACT GTGGAGACTC AGAACGTATG TCTCCCCCCT 
CCTTCCTCAG CAGGAGGGGA CAATAACAGG GGTCTTCAGG AGGAGATGCT CCACCTGAGG 
GCTGAGATCC ACCAGCACTT AGAGGAGAAG AGGAGAGCTG AGGGGGAACT GAAGGAGCTA 
AAGGCTCAAA TTGAGGAAGC AGGATTCTCC TCTGTGGCCC ACATCAGGAA CACCATGCTG 
AGCCTTTGCC TTGAGAATGC AGAGCTGAAA GAGCAGATGG GAGAAGCAAT GTCTGATGGA 
TGGGAGATCG AGGAAGACAA GGAGAAGGGC GAGGTGACAG TGGAAACTGT GGCAGCCAAA 
GGAGGTCTGA ATGAAAATAG CCTTCAGGCT GAGTTCAGAA AGCTCCAGGG AAAACTGAAG 
CATGCCCACA ACATTATCAA CCTCCTCAAA GAGCAGCTGG TGCTGAGCAG CAAGGATGGG 
AATAGTAAAC TTAGTCCAGA GCTCCTAGTG CGCCTGGACC GGGAGATCGA CAGAATGAAC 
ACAGAGCTGG TCTGTTCTCC GGAGAAGCCC CAGTGCCAGG AAGAAGAGCA TATGACCACA 
AGGCCCTGCC CCAGACCCCA GAGCCTTGAC CTTGGGGCTA CCCTCTCAGT GGATGGCCAC 
CAACTGGATA ACCGGCCCCA GGCCCAAGAC CCTGGGCCTC AGTCAGAATT TAGCCTTCCT 
GGATCCACCA AGCACCTGCG CTCCCAGCTG GCCCAATGCA GACAACGCTA TCAGGATCTC 
CAGGAGAAGC TGCTGATATC GGAAGCCACA GTCTTTGCAC AGGCCAACCA ACTGGAGAAA 
TACCGAGTCA TGCTTAGTGA ATCCTTGGTG AAGCAGGACA GCAAGCAGGT GCAGGTGGAC 
CTCCAGGATC TGGGCTATGA AACCTGTGGC CGAAGTGAGA ATGAGGCTGA GCGGGAGGAG 
AGCACCAGTC CCGAGTGTGA GGAGCCTGAC AGCCTCAGGG AAACAGTCCT GATGGTGGGG 
CACCTGGGCT CACTGCTGGC CAGCCCTCCA GGGAAGAAGC CCTTGGAAAG CCATCGGGGG 
AAGCAGGAGG AGTTCCAGGC ATATGGAAAG TCAGAAGACA TCTCTGTCCT ACATAAGGAC 
ATCAGAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGA TCATTCAGAA TCTTAAGAGC 
CGGGTCCGGT CCCTCTCAGT TACAAGTGAT TATTCATCTA GTCTGGAGAG ACCAAGAAAG 
CTGAAGGCCA TTGGCACCCT TGAGGGATCC TCACCTCACA GTGTCACTGA CGAGGATGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TGCACCCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG AGTGTCTCAG CTGGAGGCCC AGCTCCCGAA AACTGGAGTG 
GAAGGGAAGC TAGCAGAGGA GCTGAGATCA GCCTCATGGC CTGGGAAATA TGATTCCCTA 
ATTCAGGATC AGGCCCGAGA GCTGTCGTAC CTACGCCAGA AGATACGAGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCATGCAAAA GATACAGTAA AATCTTTTGA GGACCTTCTT 
AGGAGCAATG ACATTGACTA CTACCTGGGC CAGAGCTTCC GTGAGCAACT TGCCCAGGGA 
AGCCAGCTGA CAGAGAGACT CACCAGTAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTTGAACC ACTGGCCCTC AGGCTCAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATTG AAGTCCTGCA GGCCAAGCTA GATGCTCGGT CTCTCACACC CTCCAGCAGC 
CATGCATTGT CAGACTCCCA CCGCTCTCCC AGCAGCTCGT CCTTCCTGTC TGATGAGCTG 
GAAGCCTGCT CTGACATGGA TGTAGCCAGC GAGTACACAC ATTATGAAGA GAAGAAGGCT 
CCACCTGGTC ACTCAGATTC CATCCATCAT TTGAGTCATT CTGCTGTACT GTCTTCTAAA 
CCATCAGCAA CCAGTGTACC TCGGGGGGTT AAGGCCGAAC CCAGCAGCAA CCCCATCAGC 
TTGCCAGCTC CCCAGAACCC CCCCCAAGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTT 
CCCTCCATAT CCACACTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCCAC TGGCCCTCCC CTCCTTGGCT GCTGTGAGAC ACCTATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCAGA AACAATTGGG AGAAAGTGTC 
AGCACCGTCC ATCCTGCTTC CCCAGCTGCA TTGCCAAGCA ACCATTTAGA AGCCAACTCT 
TCCCAATATC TCAACCCTGC CCAGCCCCAC TCTCCTCCAA CTGGCAGCAC AGAATTGGGC 
AGAATCCTGG AGCCTGGCTA CCTGGGTAGC GGCAGCCAGT GGGATGTGAT GAGGCCTCAG 
AAGGGGAGTG TATCAGGCGA CTTATCCTCA GGATCCTCTG TGTGTCAGCT TAACTCCAAA 
CCCACAGGGG CTGACCTGCT AGAAGAACAT CTTGGTGAAA TCCGGAACCT GCGCCAGCGC 
CTAGAGGAGT CCATCTGCAT CAATGACCGC CTGCGGGAAC AGCTGGAACA CCGGCTCAGC 
TGCACAGCAC GTGGCAATGG ATCCACCTCT AATTTCTACA GTCCTGGCCT GGAACCCACA 
CCTCAGCTCT ACAATGAGAA CAGAGTCCTT AAGGAAGAAA ACCGGAGCCT TCAGGCTCAG 
CTGAGTCATG TTTCTAGAGA GCGCTCCCAG GAGACAGAAT GCCTGAGGGA GGCTCTACTG 
TCCTCTCAAT CCCGCCTTCA AGAGCTGGAA ATGGAGCTAG AGCACCAAAA GGAGGAACGG 
CAGCAGCTTT TGGAAGACTT GAAGGAGAAG CAGCAGGAGA TCTTGCACTT CCAGGAGGAG 
CGCCTGTCCC TCCAGGAGAA AGACTCTAGG CTGCAGCGTA AGCTGGCCCT ACTGCAGCAA 
CAGTGTGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCGCTTTATG GCAGTTCCAA GAAGGGTCTG AAAGCTTCCA CTTGGGATGC CTATCACCAG 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAAA GCATACAGGT GAATAACTGT CTGCGACTGC AGCTGGAGCA GCAGCTGGAT 
GGCGGAAGGA GCAAAACCAG CCTCAGTCCC TCTTCCACCA GCCAGGAGGA CCCTGGAGAC 
AAGCAAGACT CAATTGCTTC CCCTCCAGTC CGAGATGTTG GCATGAATTC TCCAGCTCTC 
ATGTTCCCTA GCTCTTCTCC TGCTGCTCCA GGCTCAGAGA CAGCTATTTT CAATAGGATA 
AATGAGCTGA ATTTGGATGC TTTTCCAGTA ATGAAGAACT CTCCCAAGCT GGAGGGCGAA 
GCTATCGATG GCTCCTTTGC CAATAAGCAC GGCCGCCATG TGATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGACAGCA GATTGGTGAG GGCAAAGTGC TGGTCAAAAA GATAACAGCA 
CTTGTGAGAT CAGCCTACAC CCTCCCTGGC CTTGAAACTC AGGCGACAGA GGTGCCAGGT 
GGCAAAGGCA TCCATGAACT TGGGAGCAGC ACCCAAGCCC TGCAGCACGT GCTGGAGGAG 
TCGGCCTCCC TCCTCAATAT GTTCTGGCGA GCGGCCCTGC CAAGCTCCAG CCCTGCCCTG 
TCTGGCAACA TGGGACAGGC CATGAAAAAG GAACTTCTGG AACTGAGAAC CAAACTGACC 
AAACAGGAGA CCCTCCTTCA GTGCACAGCT GAGCGTCTAA AGACCACCCA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAT TTGACCAGGA CACATGATGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGGC CAAGTCAGGA ATCCGAGGAA AGCAAAAGAA CACTTACAAG 
ATGGCCTCTG TAAAGCCTTA TTCCTGCCCC AGCAAAGGAG CCACGCAAGA AGAGGAGCCC 
CCAGAGGTCC TTCAAACAGC AGGAAGGATG GGCCTGCCCC CCTTTTGTGC AGGTCCCAAT 
CTGCTGAGGA GGCTTGGACC CCACTCCTCC GCACTGGCTG GAGGGTCCAG GAGGAGTCAG 
GCATGGCCCA GTGGACGAGG GGGAAAGGAA GGCAGCCTTT CCTGGGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120601.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976076.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X17, mRNA.

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CloneID	OCe40887
Clone ID Related Accession (Same CDS sequence)	XM_014120601.1 , XM_014120601.2
Accession Version	XM_014120601.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CATCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA TTTCCTGGAG GAACGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGAGGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGG 
GAACTCCAGG ACAAGAAACA GCATCTAGAT AAAACGTGGG CTGATGTGGA GAATCTCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGTCAG TTTGAAGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAGG AGGAATCCAG GCTAGCAAAG 
AATGAGGCTG CACGGATGGC AGCTCTGGTG GAAGCTGAGA AAGAATGTAA CCTGGAGCTA 
TCAGAGAAAC TGAAGGGAGT TGCCAATGAC AGGGAAGAGG TACCAATGGA CCAGGTCAAT 
CCTGACCAAT ACACTGAGGC CCTGGCCCAG CGGGACAAAA GAATTGAAGA ACTTCATCAA 
AGCCTGGCTT CCCAGGAGAA GCTTGTAGAA CAGCTGTCTC AAGAGAAACA ACAACTGCTC 
CATCTGTTGG AGGAACCAAT GAGCATGGAA ATACAGCCTG TGACTGAAGA GTTACTCAAG 
CAACAAAAGC TGGATTCAGA TGAGACCATT ATAACTCAGC AGTCTGTATC TGATTCCCAC 
TTGGCTGAAC TCCAGGAAAA AATCCAGCAA ATGGAGGCCA CCAACAAAAT TCTTCAAGAG 
AAACTGAATG AAATGAGCTG TGAGCTAAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GGTACAATTC AAAGCCTCAA GGAAACTCTG AAAAGTAGGG AAAATGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAATGACACA ATGGCAAAGC TTCGAGAAAT GCTACACCAA 
AGCCAGCTTG GACAACTTCA TGTATTTGAG GGTCACATAG GACAGGAGAG CTCACAGGGT 
ACTTCCCCAG CTCAGCAACA GATGGCTCTG CTTGATCTTC AGAGTGGTTT GTTCTACAGC 
CAGCTTGAGA TTCAGAAACT CCAGAGGTTG GTACGTCAAA AAGAACGCCA ACTGGCTGAT 
GCCAAACGAT GTGTCCAATT TGTAGAGGCT GCAGCCCATG AAAGAGAACA GCAGAAAGAG 
GCATCTTGGA AACATAATCA GGAATTACGG AAAGCCTTGC AGCAGCTCCA AGGAGAATTA 
GAGAGTAAGA GCCAACAGCT TCGTACCCTG GAGACTGAAA AATGTAATGA GATCAGAGCC 
CATGAGCAAC ACATTCAACA CCTAAACCAT AGTCTGAGCC ACAAAGAGCA GCTGCTTCAG 
GAATTTCGGG AGCTCCTGCA GTATCGAGAT AAGTCAGACA AAACCCTTGA AGCAAATGAA 
ATGTTGCTTG AGAAACTTCG GCAGCGAATA CAAGACCGAG ATGTTGCTTT GGAGCGGGCT 
ATAGATGAAA AGTTCTCCGC CCTGGAAGAG AAAGAAAAAG AACTGCGTCA ATTTCATCTT 
GCTGTGCGAG AGCGTGATCA TGACTTAGAG AGATTGCGTG CTATTCTCTC CTCTAATGAA 
GCTACCATGC AGAGTATGGA GAGTCTCCTG AGGGCCAAAG GCCTAGAAGT AGAGCAGTTA 
TCAGCTACCT GTCAAAACCT CCAGTGGCTG AAAGAAGAAA AGGAAACCAA ATTTAGCCAT 
TGGCAGAAGG AACAAGAAAG TATCATTCAG CAGTTACAGA CATCTCTGCA TGACAGGAAC 
AAAGAAGTGG AGGATCTGAG TGCCACCTTG TTCTGTAAGC TTGGACCTGG GCAGAGTGAG 
GTAGCAGAAG AGCTGTGCCA GCGCCTACAG CGAAAGGAAA GGATGCTACA GGAACTGCTA 
ACCGATCGAA ACAAACAAGC AGTGGAACAC GAAATAGAGA TTCAAGGCCT GCTTCAGTCT 
ATGAGCACCA GGGAGCAGGA GAGCCAAGCC GCTGCAGGGA AGATGGTGCA AGCCCTTATG 
GAAAGAAATT CAGAACTACA GGCCCTGCGC CAGTATTTAG GAGGTAAAGA CTTCATGGTT 
TCCCAAGCAC TCATCTCTAA CCAACCCACA GAAGTCACCT CCAACAGCCC TCGTCTTGGA 
GAGCAGACTG ATCAAGGTTC AGTGCATATA CCCTCCAAAG ATGATAGCAT TTCCCTGACT 
GCCAAAGGGG ATACCAGTAT ACCCAGGTCC TCATTAGGAG ACCAGGATAC AGTTGCAGGG 
CTGGAAAAAG AACTGAGTAA TGCCAAAGAG GAGCTTGAGC TCATGGCTAA GAAAGAACGA 
GAAAGTCGGA TGGAACTTTC TGCACTACAG TCCATGGTGG CCGTGCAAGA GGAAGAGCTG 
CAGGTGCAGG CTGCTGACAT GGAATCCCTG ACCAGGAATA TACAGATTAA AGAAGACCTC 
ATAAAGGACC TGCAGATGCA ACTGGTTGAT CCTGAAGATA TACCAGCTAT GGAACGCCTG 
ACCCAAGAGG TCTTACTTCT TCGGGAAAAA GTTGCTTCAG TAGAATCACA GGGTCAGGAA 
ACGTTAGGAA ATCGAAGACA ACAATTGCTG CTAATGCTCG AAGGGCTGGT GGATGAACGG 
AGTCGGCTCA ATGAAGCTTT ACAAGCAGAG AGACAGCTGT ACAGCAGCCT GGTGAAGTTC 
CATGCCCATC CAGAGAGCTC TGAGAGAGAC CGAACTCTGC AGGTGGAACT AGAAGGGGCC 
CAGGTGATAC GTGGTCGGCT AGAAGAAGTT CTTGGAAGAA GCCTGGAGCG CTTAAGCAGG 
CTGGAGACCC TGGCTGCCAT TGGAGGTGCA GCTGCAGGGG AAGATACAGA AGATGCTAGC 
ACTGAGTTCA CGGACAGCAT CGAGGAGGAG GCTGCACACC ATAATCACCA GCAACTCATC 
AAGGTGGCTT TGGAGAAAAG TCTGGCAACT GTGGAGACTC AGAACGTATG TCTCCCCCCT 
CCTTCCTCAG CAGGAGGGGA CAATAACAGG GGTCTTCAGG AGGAGATGCT CCACCTGAGG 
GCTGAGATCC ACCAGCACTT AGAGGAGAAG AGGAGAGCTG AGGGGGAACT GAAGGAGCTA 
AAGGCTCAAA TTGAGGAAGC AGGATTCTCC TCTGTGGCCC ACATCAGGAA CACCATGCTG 
AGCCTTTGCC TTGAGAATGC AGAGCTGAAA GAGCAGATGG GAGAAGCAAT GTCTGATGGA 
TGGGAGATCG AGGAAGACAA GGAGAAGGGC GAGGTGACAG TGGAAACTGT GGCAGCCAAA 
GGAGGTCTGA ATGAAAATAG CCTTCAGGCT GAGTTCAGAA AGCTCCAGGG AAAACTGAAG 
CATGCCCACA ACATTATCAA CCTCCTCAAA GAGCAGCTGG TGCTGAGCAG CAAGGATGGG 
AATAGTAAAC TTAGTCCAGA GCTCCTAGTG CGCCTGGACC GGGAGATCGA CAGAATGAAC 
ACAGAGCTGG TCTGTTCTCC GGAGAAGCCC CAGTGCCAGG AAGAAGAGCA TATGACCACA 
AGGCCCTGCC CCAGACCCCA GAGCCTTGAC CTTGGGGCTA CCCTCTCAGT GGATGGCCAC 
CAACTGGATA ACCGGCCCCA GGCCCAAGAC CCTGGGCCTC AGTCAGAATT TAGCCTTCCT 
GGATCCACCA AGCACCTGCG CTCCCAGCTG GCCCAATGCA GACAACGCTA TCAGGATCTC 
CAGGAGAAGC TGCTGATATC GGAAGCCACA GTCTTTGCAC AGGCCAACCA ACTGGAGAAA 
TACCGAGTCA TGCTTAGTGA ATCCTTGGTG AAGCAGGACA GCAAGCAGGT GCAGGTGGAC 
CTCCAGGATC TGGGCTATGA AACCTGTGGC CGAAGTGAGA ATGAGGCTGA GCGGGAGGAG 
AGCACCAGTC CCGAGTGTGA GGAGCCTGAC AGCCTCAGGG AAACAGTCCT GATGGTGGGG 
CACCTGGGCT CACTGCTGGC CAGCCCTCCA GGGAAGAAGC CCTTGGAAAG CCATCGGGGG 
AAGCAGGAGG AGTTCCAGGC ATATGGAAAG TCAGAAGACA TCTCTGTCCT ACATAAGGAC 
ATCAGAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGA TCATTCAGAA TCTTAAGAGC 
CGGGTCCGGT CCCTCTCAGT TACAAGTGAT TATTCATCTA GTCTGGAGAG ACCAAGAAAG 
CTGAAGGCCA TTGGCACCCT TGAGGGATCC TCACCTCACA GTGTCACTGA CGAGGATGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TGCACCCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG AGTGTCTCAG CTGGAGGCCC AGCTCCCGAA AACTGGAGTG 
GAAGGGAAGC TAGCAGAGGA GCTGAGATCA GCCTCATGGC CTGGGAAATA TGATTCCCTA 
ATTCAGGATC AGGCCCGAGA GCTGTCGTAC CTACGCCAGA AGATACGAGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCATGCAAAA GATACAGTAA AATCTTTTGA GGACCTTCTT 
AGGAGCAATG ACATTGACTA CTACCTGGGC CAGAGCTTCC GTGAGCAACT TGCCCAGGGA 
AGCCAGCTGA CAGAGAGACT CACCAGTAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTTGAACC ACTGGCCCTC AGGCTCAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATTG AAGTCCTGCA GGCCAAGCTA GATGCTCGGT CTCTCACACC CTCCAGCAGC 
CATGCATTGT CAGACTCCCA CCGCTCTCCC AGCAGCTCGT CCTTCCTGTC TGATGAGCTG 
GAAGCCTGCT CTGACATGGA TGTAGCCAGC GAGTACACAC ATTATGAAGA GAAGAAGGCT 
CCACCTGGTC ACTCAGATTC CATCCATCAT TTGAGTCATT CTGCTGTACT GTCTTCTAAA 
CCATCAGCAA CCAGTGTACC TCGGGGGGTT AAGGCCGAAC CCAGCAGCAA CCCCATCAGC 
TTGCCAGCTC CCCAGAACCC CCCCCAAGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTT 
CCCTCCATAT CCACACTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCCAC TGGCCCTCCC CTCCTTGGCT GCTGTGAGAC ACCTATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCAGA AACAATTGGG AGAAAGTGTC 
AGCACCGTCC ATCCTGCTTC CCCAGCTGCA TTGCCAAGCA ACCATTTAGA AGCCAACTCT 
TCCCAATATC TCAACCCTGC CCAGCCCCAC TCTCCTCCAA CTGGCAGCAC AGAATTGGGC 
AGAATCCTGG AGCCTGGCTA CCTGGGTAGC GGCAGCCAGT GGGATGTGAT GAGGCCTCAG 
AAGGGGAGTG TATCAGGCGA CTTATCCTCA GGATCCTCTG TGTGTCAGCT TAACTCCAAA 
CCCACAGGGG CTGACCTGCT AGAAGAACAT CTTGGTGAAA TCCGGAACCT GCGCCAGCGC 
CTAGAGGAGT CCATCTGCAT CAATGACCGC CTGCGGGAAC AGCTGGAACA CCGGCTCAGC 
TGCACAGCAC GTGGCAATGG ATCCACCTCT AATTTCTACA GTCCTGGCCT GGAACCCACA 
CCTCAGCTCT ACAATGAGAA CAGAGTCCTT AAGGAAGAAA ACCGGAGCCT TCAGGCTCAG 
CTGAGTCATG TTTCTAGAGA GCGCTCCCAG GAGACAGAAT GCCTGAGGGA GGCTCTACTG 
TCCTCTCAAT CCCGCCTTCA AGAGCTGGAA ATGGAGCTAG AGCACCAAAA GGAGGAACGG 
CAGCAGCTTT TGGAAGACTT GAAGGAGAAG CAGCAGGAGA TCTTGCACTT CCAGGAGGAG 
CGCCTGTCCC TCCAGGAGAA AGACTCTAGG CTGCAGCGTA AGCTGGCCCT ACTGCAGCAA 
CAGTGTGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCGCTTTATG GCAGTTCCAA GAAGGGTCTG AAAGCTTCCA CTTGGGATGC CTATCACCAG 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAAA GCATACAGGT GAATAACTGT CTGCGACTGC AGCTGGAGCA GCAGCTGGAT 
GGCGGAAGGA GCAAAACCAG CCTCAGTCCC TCTTCCACCA GCCAGGAGGA CCCTGGAGAC 
AAGCAAGACT CAATTGCTTC CCCTCCAGTC CGAGATGTTG GCATGAATTC TCCAGCTCTC 
ATGTTCCCTA GCTCTTCTCC TGCTGCTCCA GGCTCAGAGA CAGCTATTTT CAATAGGATA 
AATGAGCTGA ATTTGGATGC TTTTCCAGTA ATGAAGAACT CTCCCAAGCT GGAGGGCGAA 
GCTATCGATG GCTCCTTTGC CAATAAGCAC GGCCGCCATG TGATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGACAGCA GATTGGTGAG GGCAAAGTGC TGGTCAAAAA GATAACAGCA 
CTTGTGAGAT CAGCCTACAC CCTCCCTGGC CTTGAAACTC AGGCGACAGA GGTGCCAGGT 
GGCAAAGGCA TCCATGAACT TGGGAGCAGC ACCCAAGCCC TGCAGCACGT GCTGGAGGAG 
TCGGCCTCCC TCCTCAATAT GTTCTGGCGA GCGGCCCTGC CAAGCTCCAG CCCTGCCCTG 
TCTGGCAACA TGGGACAGGC CATGAAAAAG GAACTTCTGG AACTGAGAAC CAAACTGACC 
AAACAGGAGA CCCTCCTTCA GTGCACAGCT GAGCGTCTAA AGACCACCCA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAT TTGACCAGGA CACATGATGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGGT GAAGTCCCTA AGGGCTCTGG CAAGCACCCC AGTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976076.1
CDS	220..7239
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120601.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120601.1

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CATCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA TTTCCTGGAG GAACGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGAGGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGG 
GAACTCCAGG ACAAGAAACA GCATCTAGAT AAAACGTGGG CTGATGTGGA GAATCTCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGTCAG TTTGAAGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAGG AGGAATCCAG GCTAGCAAAG 
AATGAGGCTG CACGGATGGC AGCTCTGGTG GAAGCTGAGA AAGAATGTAA CCTGGAGCTA 
TCAGAGAAAC TGAAGGGAGT TGCCAATGAC AGGGAAGAGG TACCAATGGA CCAGGTCAAT 
CCTGACCAAT ACACTGAGGC CCTGGCCCAG CGGGACAAAA GAATTGAAGA ACTTCATCAA 
AGCCTGGCTT CCCAGGAGAA GCTTGTAGAA CAGCTGTCTC AAGAGAAACA ACAACTGCTC 
CATCTGTTGG AGGAACCAAT GAGCATGGAA ATACAGCCTG TGACTGAAGA GTTACTCAAG 
CAACAAAAGC TGGATTCAGA TGAGACCATT ATAACTCAGC AGTCTGTATC TGATTCCCAC 
TTGGCTGAAC TCCAGGAAAA AATCCAGCAA ATGGAGGCCA CCAACAAAAT TCTTCAAGAG 
AAACTGAATG AAATGAGCTG TGAGCTAAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GGTACAATTC AAAGCCTCAA GGAAACTCTG AAAAGTAGGG AAAATGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAATGACACA ATGGCAAAGC TTCGAGAAAT GCTACACCAA 
AGCCAGCTTG GACAACTTCA TGTATTTGAG GGTCACATAG GACAGGAGAG CTCACAGGGT 
ACTTCCCCAG CTCAGCAACA GATGGCTCTG CTTGATCTTC AGAGTGGTTT GTTCTACAGC 
CAGCTTGAGA TTCAGAAACT CCAGAGGTTG GTACGTCAAA AAGAACGCCA ACTGGCTGAT 
GCCAAACGAT GTGTCCAATT TGTAGAGGCT GCAGCCCATG AAAGAGAACA GCAGAAAGAG 
GCATCTTGGA AACATAATCA GGAATTACGG AAAGCCTTGC AGCAGCTCCA AGGAGAATTA 
GAGAGTAAGA GCCAACAGCT TCGTACCCTG GAGACTGAAA AATGTAATGA GATCAGAGCC 
CATGAGCAAC ACATTCAACA CCTAAACCAT AGTCTGAGCC ACAAAGAGCA GCTGCTTCAG 
GAATTTCGGG AGCTCCTGCA GTATCGAGAT AAGTCAGACA AAACCCTTGA AGCAAATGAA 
ATGTTGCTTG AGAAACTTCG GCAGCGAATA CAAGACCGAG ATGTTGCTTT GGAGCGGGCT 
ATAGATGAAA AGTTCTCCGC CCTGGAAGAG AAAGAAAAAG AACTGCGTCA ATTTCATCTT 
GCTGTGCGAG AGCGTGATCA TGACTTAGAG AGATTGCGTG CTATTCTCTC CTCTAATGAA 
GCTACCATGC AGAGTATGGA GAGTCTCCTG AGGGCCAAAG GCCTAGAAGT AGAGCAGTTA 
TCAGCTACCT GTCAAAACCT CCAGTGGCTG AAAGAAGAAA AGGAAACCAA ATTTAGCCAT 
TGGCAGAAGG AACAAGAAAG TATCATTCAG CAGTTACAGA CATCTCTGCA TGACAGGAAC 
AAAGAAGTGG AGGATCTGAG TGCCACCTTG TTCTGTAAGC TTGGACCTGG GCAGAGTGAG 
GTAGCAGAAG AGCTGTGCCA GCGCCTACAG CGAAAGGAAA GGATGCTACA GGAACTGCTA 
ACCGATCGAA ACAAACAAGC AGTGGAACAC GAAATAGAGA TTCAAGGCCT GCTTCAGTCT 
ATGAGCACCA GGGAGCAGGA GAGCCAAGCC GCTGCAGGGA AGATGGTGCA AGCCCTTATG 
GAAAGAAATT CAGAACTACA GGCCCTGCGC CAGTATTTAG GAGGTAAAGA CTTCATGGTT 
TCCCAAGCAC TCATCTCTAA CCAACCCACA GAAGTCACCT CCAACAGCCC TCGTCTTGGA 
GAGCAGACTG ATCAAGGTTC AGTGCATATA CCCTCCAAAG ATGATAGCAT TTCCCTGACT 
GCCAAAGGGG ATACCAGTAT ACCCAGGTCC TCATTAGGAG ACCAGGATAC AGTTGCAGGG 
CTGGAAAAAG AACTGAGTAA TGCCAAAGAG GAGCTTGAGC TCATGGCTAA GAAAGAACGA 
GAAAGTCGGA TGGAACTTTC TGCACTACAG TCCATGGTGG CCGTGCAAGA GGAAGAGCTG 
CAGGTGCAGG CTGCTGACAT GGAATCCCTG ACCAGGAATA TACAGATTAA AGAAGACCTC 
ATAAAGGACC TGCAGATGCA ACTGGTTGAT CCTGAAGATA TACCAGCTAT GGAACGCCTG 
ACCCAAGAGG TCTTACTTCT TCGGGAAAAA GTTGCTTCAG TAGAATCACA GGGTCAGGAA 
ACGTTAGGAA ATCGAAGACA ACAATTGCTG CTAATGCTCG AAGGGCTGGT GGATGAACGG 
AGTCGGCTCA ATGAAGCTTT ACAAGCAGAG AGACAGCTGT ACAGCAGCCT GGTGAAGTTC 
CATGCCCATC CAGAGAGCTC TGAGAGAGAC CGAACTCTGC AGGTGGAACT AGAAGGGGCC 
CAGGTGATAC GTGGTCGGCT AGAAGAAGTT CTTGGAAGAA GCCTGGAGCG CTTAAGCAGG 
CTGGAGACCC TGGCTGCCAT TGGAGGTGCA GCTGCAGGGG AAGATACAGA AGATGCTAGC 
ACTGAGTTCA CGGACAGCAT CGAGGAGGAG GCTGCACACC ATAATCACCA GCAACTCATC 
AAGGTGGCTT TGGAGAAAAG TCTGGCAACT GTGGAGACTC AGAACGTATG TCTCCCCCCT 
CCTTCCTCAG CAGGAGGGGA CAATAACAGG GGTCTTCAGG AGGAGATGCT CCACCTGAGG 
GCTGAGATCC ACCAGCACTT AGAGGAGAAG AGGAGAGCTG AGGGGGAACT GAAGGAGCTA 
AAGGCTCAAA TTGAGGAAGC AGGATTCTCC TCTGTGGCCC ACATCAGGAA CACCATGCTG 
AGCCTTTGCC TTGAGAATGC AGAGCTGAAA GAGCAGATGG GAGAAGCAAT GTCTGATGGA 
TGGGAGATCG AGGAAGACAA GGAGAAGGGC GAGGTGACAG TGGAAACTGT GGCAGCCAAA 
GGAGGTCTGA ATGAAAATAG CCTTCAGGCT GAGTTCAGAA AGCTCCAGGG AAAACTGAAG 
CATGCCCACA ACATTATCAA CCTCCTCAAA GAGCAGCTGG TGCTGAGCAG CAAGGATGGG 
AATAGTAAAC TTAGTCCAGA GCTCCTAGTG CGCCTGGACC GGGAGATCGA CAGAATGAAC 
ACAGAGCTGG TCTGTTCTCC GGAGAAGCCC CAGTGCCAGG AAGAAGAGCA TATGACCACA 
AGGCCCTGCC CCAGACCCCA GAGCCTTGAC CTTGGGGCTA CCCTCTCAGT GGATGGCCAC 
CAACTGGATA ACCGGCCCCA GGCCCAAGAC CCTGGGCCTC AGTCAGAATT TAGCCTTCCT 
GGATCCACCA AGCACCTGCG CTCCCAGCTG GCCCAATGCA GACAACGCTA TCAGGATCTC 
CAGGAGAAGC TGCTGATATC GGAAGCCACA GTCTTTGCAC AGGCCAACCA ACTGGAGAAA 
TACCGAGTCA TGCTTAGTGA ATCCTTGGTG AAGCAGGACA GCAAGCAGGT GCAGGTGGAC 
CTCCAGGATC TGGGCTATGA AACCTGTGGC CGAAGTGAGA ATGAGGCTGA GCGGGAGGAG 
AGCACCAGTC CCGAGTGTGA GGAGCCTGAC AGCCTCAGGG AAACAGTCCT GATGGTGGGG 
CACCTGGGCT CACTGCTGGC CAGCCCTCCA GGGAAGAAGC CCTTGGAAAG CCATCGGGGG 
AAGCAGGAGG AGTTCCAGGC ATATGGAAAG TCAGAAGACA TCTCTGTCCT ACATAAGGAC 
ATCAGAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGA TCATTCAGAA TCTTAAGAGC 
CGGGTCCGGT CCCTCTCAGT TACAAGTGAT TATTCATCTA GTCTGGAGAG ACCAAGAAAG 
CTGAAGGCCA TTGGCACCCT TGAGGGATCC TCACCTCACA GTGTCACTGA CGAGGATGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TGCACCCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG AGTGTCTCAG CTGGAGGCCC AGCTCCCGAA AACTGGAGTG 
GAAGGGAAGC TAGCAGAGGA GCTGAGATCA GCCTCATGGC CTGGGAAATA TGATTCCCTA 
ATTCAGGATC AGGCCCGAGA GCTGTCGTAC CTACGCCAGA AGATACGAGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCATGCAAAA GATACAGTAA AATCTTTTGA GGACCTTCTT 
AGGAGCAATG ACATTGACTA CTACCTGGGC CAGAGCTTCC GTGAGCAACT TGCCCAGGGA 
AGCCAGCTGA CAGAGAGACT CACCAGTAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTTGAACC ACTGGCCCTC AGGCTCAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATTG AAGTCCTGCA GGCCAAGCTA GATGCTCGGT CTCTCACACC CTCCAGCAGC 
CATGCATTGT CAGACTCCCA CCGCTCTCCC AGCAGCTCGT CCTTCCTGTC TGATGAGCTG 
GAAGCCTGCT CTGACATGGA TGTAGCCAGC GAGTACACAC ATTATGAAGA GAAGAAGGCT 
CCACCTGGTC ACTCAGATTC CATCCATCAT TTGAGTCATT CTGCTGTACT GTCTTCTAAA 
CCATCAGCAA CCAGTGTACC TCGGGGGGTT AAGGCCGAAC CCAGCAGCAA CCCCATCAGC 
TTGCCAGCTC CCCAGAACCC CCCCCAAGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTT 
CCCTCCATAT CCACACTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCCAC TGGCCCTCCC CTCCTTGGCT GCTGTGAGAC ACCTATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCAGA AACAATTGGG AGAAAGTGTC 
AGCACCGTCC ATCCTGCTTC CCCAGCTGCA TTGCCAAGCA ACCATTTAGA AGCCAACTCT 
TCCCAATATC TCAACCCTGC CCAGCCCCAC TCTCCTCCAA CTGGCAGCAC AGAATTGGGC 
AGAATCCTGG AGCCTGGCTA CCTGGGTAGC GGCAGCCAGT GGGATGTGAT GAGGCCTCAG 
AAGGGGAGTG TATCAGGCGA CTTATCCTCA GGATCCTCTG TGTGTCAGCT TAACTCCAAA 
CCCACAGGGG CTGACCTGCT AGAAGAACAT CTTGGTGAAA TCCGGAACCT GCGCCAGCGC 
CTAGAGGAGT CCATCTGCAT CAATGACCGC CTGCGGGAAC AGCTGGAACA CCGGCTCAGC 
TGCACAGCAC GTGGCAATGG ATCCACCTCT AATTTCTACA GTCCTGGCCT GGAACCCACA 
CCTCAGCTCT ACAATGAGAA CAGAGTCCTT AAGGAAGAAA ACCGGAGCCT TCAGGCTCAG 
CTGAGTCATG TTTCTAGAGA GCGCTCCCAG GAGACAGAAT GCCTGAGGGA GGCTCTACTG 
TCCTCTCAAT CCCGCCTTCA AGAGCTGGAA ATGGAGCTAG AGCACCAAAA GGAGGAACGG 
CAGCAGCTTT TGGAAGACTT GAAGGAGAAG CAGCAGGAGA TCTTGCACTT CCAGGAGGAG 
CGCCTGTCCC TCCAGGAGAA AGACTCTAGG CTGCAGCGTA AGCTGGCCCT ACTGCAGCAA 
CAGTGTGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCGCTTTATG GCAGTTCCAA GAAGGGTCTG AAAGCTTCCA CTTGGGATGC CTATCACCAG 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAAA GCATACAGGT GAATAACTGT CTGCGACTGC AGCTGGAGCA GCAGCTGGAT 
GGCGGAAGGA GCAAAACCAG CCTCAGTCCC TCTTCCACCA GCCAGGAGGA CCCTGGAGAC 
AAGCAAGACT CAATTGCTTC CCCTCCAGTC CGAGATGTTG GCATGAATTC TCCAGCTCTC 
ATGTTCCCTA GCTCTTCTCC TGCTGCTCCA GGCTCAGAGA CAGCTATTTT CAATAGGATA 
AATGAGCTGA ATTTGGATGC TTTTCCAGTA ATGAAGAACT CTCCCAAGCT GGAGGGCGAA 
GCTATCGATG GCTCCTTTGC CAATAAGCAC GGCCGCCATG TGATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGACAGCA GATTGGTGAG GGCAAAGTGC TGGTCAAAAA GATAACAGCA 
CTTGTGAGAT CAGCCTACAC CCTCCCTGGC CTTGAAACTC AGGCGACAGA GGTGCCAGGT 
GGCAAAGGCA TCCATGAACT TGGGAGCAGC ACCCAAGCCC TGCAGCACGT GCTGGAGGAG 
TCGGCCTCCC TCCTCAATAT GTTCTGGCGA GCGGCCCTGC CAAGCTCCAG CCCTGCCCTG 
TCTGGCAACA TGGGACAGGC CATGAAAAAG GAACTTCTGG AACTGAGAAC CAAACTGACC 
AAACAGGAGA CCCTCCTTCA GTGCACAGCT GAGCGTCTAA AGACCACCCA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAT TTGACCAGGA CACATGATGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGGT GAAGTCCCTA AGGGCTCTGG CAAGCACCCC AGTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120601.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976076.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X17, mRNA.

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CloneID	OCe40887
Clone ID Related Accession (Same CDS sequence)	XM_014120601.1 , XM_014120601.2
Accession Version	XM_014120601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CATCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA TTTCCTGGAG GAACGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGAGGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGG 
GAACTCCAGG ACAAGAAACA GCATCTAGAT AAAACGTGGG CTGATGTGGA GAATCTCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGTCAG TTTGAAGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAGG AGGAATCCAG GCTAGCAAAG 
AATGAGGCTG CACGGATGGC AGCTCTGGTG GAAGCTGAGA AAGAATGTAA CCTGGAGCTA 
TCAGAGAAAC TGAAGGGAGT TGCCAATGAC AGGGAAGAGG TACCAATGGA CCAGGTCAAT 
CCTGACCAAT ACACTGAGGC CCTGGCCCAG CGGGACAAAA GAATTGAAGA ACTTCATCAA 
AGCCTGGCTT CCCAGGAGAA GCTTGTAGAA CAGCTGTCTC AAGAGAAACA ACAACTGCTC 
CATCTGTTGG AGGAACCAAT GAGCATGGAA ATACAGCCTG TGACTGAAGA GTTACTCAAG 
CAACAAAAGC TGGATTCAGA TGAGACCATT ATAACTCAGC AGTCTGTATC TGATTCCCAC 
TTGGCTGAAC TCCAGGAAAA AATCCAGCAA ATGGAGGCCA CCAACAAAAT TCTTCAAGAG 
AAACTGAATG AAATGAGCTG TGAGCTAAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GGTACAATTC AAAGCCTCAA GGAAACTCTG AAAAGTAGGG AAAATGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAATGACACA ATGGCAAAGC TTCGAGAAAT GCTACACCAA 
AGCCAGCTTG GACAACTTCA TGTATTTGAG GGTCACATAG GACAGGAGAG CTCACAGGGT 
ACTTCCCCAG CTCAGCAACA GATGGCTCTG CTTGATCTTC AGAGTGGTTT GTTCTACAGC 
CAGCTTGAGA TTCAGAAACT CCAGAGGTTG GTACGTCAAA AAGAACGCCA ACTGGCTGAT 
GCCAAACGAT GTGTCCAATT TGTAGAGGCT GCAGCCCATG AAAGAGAACA GCAGAAAGAG 
GCATCTTGGA AACATAATCA GGAATTACGG AAAGCCTTGC AGCAGCTCCA AGGAGAATTA 
GAGAGTAAGA GCCAACAGCT TCGTACCCTG GAGACTGAAA AATGTAATGA GATCAGAGCC 
CATGAGCAAC ACATTCAACA CCTAAACCAT AGTCTGAGCC ACAAAGAGCA GCTGCTTCAG 
GAATTTCGGG AGCTCCTGCA GTATCGAGAT AAGTCAGACA AAACCCTTGA AGCAAATGAA 
ATGTTGCTTG AGAAACTTCG GCAGCGAATA CAAGACCGAG ATGTTGCTTT GGAGCGGGCT 
ATAGATGAAA AGTTCTCCGC CCTGGAAGAG AAAGAAAAAG AACTGCGTCA ATTTCATCTT 
GCTGTGCGAG AGCGTGATCA TGACTTAGAG AGATTGCGTG CTATTCTCTC CTCTAATGAA 
GCTACCATGC AGAGTATGGA GAGTCTCCTG AGGGCCAAAG GCCTAGAAGT AGAGCAGTTA 
TCAGCTACCT GTCAAAACCT CCAGTGGCTG AAAGAAGAAA AGGAAACCAA ATTTAGCCAT 
TGGCAGAAGG AACAAGAAAG TATCATTCAG CAGTTACAGA CATCTCTGCA TGACAGGAAC 
AAAGAAGTGG AGGATCTGAG TGCCACCTTG TTCTGTAAGC TTGGACCTGG GCAGAGTGAG 
GTAGCAGAAG AGCTGTGCCA GCGCCTACAG CGAAAGGAAA GGATGCTACA GGAACTGCTA 
ACCGATCGAA ACAAACAAGC AGTGGAACAC GAAATAGAGA TTCAAGGCCT GCTTCAGTCT 
ATGAGCACCA GGGAGCAGGA GAGCCAAGCC GCTGCAGGGA AGATGGTGCA AGCCCTTATG 
GAAAGAAATT CAGAACTACA GGCCCTGCGC CAGTATTTAG GAGGTAAAGA CTTCATGGTT 
TCCCAAGCAC TCATCTCTAA CCAACCCACA GAAGTCACCT CCAACAGCCC TCGTCTTGGA 
GAGCAGACTG ATCAAGGTTC AGTGCATATA CCCTCCAAAG ATGATAGCAT TTCCCTGACT 
GCCAAAGGGG ATACCAGTAT ACCCAGGTCC TCATTAGGAG ACCAGGATAC AGTTGCAGGG 
CTGGAAAAAG AACTGAGTAA TGCCAAAGAG GAGCTTGAGC TCATGGCTAA GAAAGAACGA 
GAAAGTCGGA TGGAACTTTC TGCACTACAG TCCATGGTGG CCGTGCAAGA GGAAGAGCTG 
CAGGTGCAGG CTGCTGACAT GGAATCCCTG ACCAGGAATA TACAGATTAA AGAAGACCTC 
ATAAAGGACC TGCAGATGCA ACTGGTTGAT CCTGAAGATA TACCAGCTAT GGAACGCCTG 
ACCCAAGAGG TCTTACTTCT TCGGGAAAAA GTTGCTTCAG TAGAATCACA GGGTCAGGAA 
ACGTTAGGAA ATCGAAGACA ACAATTGCTG CTAATGCTCG AAGGGCTGGT GGATGAACGG 
AGTCGGCTCA ATGAAGCTTT ACAAGCAGAG AGACAGCTGT ACAGCAGCCT GGTGAAGTTC 
CATGCCCATC CAGAGAGCTC TGAGAGAGAC CGAACTCTGC AGGTGGAACT AGAAGGGGCC 
CAGGTGATAC GTGGTCGGCT AGAAGAAGTT CTTGGAAGAA GCCTGGAGCG CTTAAGCAGG 
CTGGAGACCC TGGCTGCCAT TGGAGGTGCA GCTGCAGGGG AAGATACAGA AGATGCTAGC 
ACTGAGTTCA CGGACAGCAT CGAGGAGGAG GCTGCACACC ATAATCACCA GCAACTCATC 
AAGGTGGCTT TGGAGAAAAG TCTGGCAACT GTGGAGACTC AGAACGTATG TCTCCCCCCT 
CCTTCCTCAG CAGGAGGGGA CAATAACAGG GGTCTTCAGG AGGAGATGCT CCACCTGAGG 
GCTGAGATCC ACCAGCACTT AGAGGAGAAG AGGAGAGCTG AGGGGGAACT GAAGGAGCTA 
AAGGCTCAAA TTGAGGAAGC AGGATTCTCC TCTGTGGCCC ACATCAGGAA CACCATGCTG 
AGCCTTTGCC TTGAGAATGC AGAGCTGAAA GAGCAGATGG GAGAAGCAAT GTCTGATGGA 
TGGGAGATCG AGGAAGACAA GGAGAAGGGC GAGGTGACAG TGGAAACTGT GGCAGCCAAA 
GGAGGTCTGA ATGAAAATAG CCTTCAGGCT GAGTTCAGAA AGCTCCAGGG AAAACTGAAG 
CATGCCCACA ACATTATCAA CCTCCTCAAA GAGCAGCTGG TGCTGAGCAG CAAGGATGGG 
AATAGTAAAC TTAGTCCAGA GCTCCTAGTG CGCCTGGACC GGGAGATCGA CAGAATGAAC 
ACAGAGCTGG TCTGTTCTCC GGAGAAGCCC CAGTGCCAGG AAGAAGAGCA TATGACCACA 
AGGCCCTGCC CCAGACCCCA GAGCCTTGAC CTTGGGGCTA CCCTCTCAGT GGATGGCCAC 
CAACTGGATA ACCGGCCCCA GGCCCAAGAC CCTGGGCCTC AGTCAGAATT TAGCCTTCCT 
GGATCCACCA AGCACCTGCG CTCCCAGCTG GCCCAATGCA GACAACGCTA TCAGGATCTC 
CAGGAGAAGC TGCTGATATC GGAAGCCACA GTCTTTGCAC AGGCCAACCA ACTGGAGAAA 
TACCGAGTCA TGCTTAGTGA ATCCTTGGTG AAGCAGGACA GCAAGCAGGT GCAGGTGGAC 
CTCCAGGATC TGGGCTATGA AACCTGTGGC CGAAGTGAGA ATGAGGCTGA GCGGGAGGAG 
AGCACCAGTC CCGAGTGTGA GGAGCCTGAC AGCCTCAGGG AAACAGTCCT GATGGTGGGG 
CACCTGGGCT CACTGCTGGC CAGCCCTCCA GGGAAGAAGC CCTTGGAAAG CCATCGGGGG 
AAGCAGGAGG AGTTCCAGGC ATATGGAAAG TCAGAAGACA TCTCTGTCCT ACATAAGGAC 
ATCAGAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGA TCATTCAGAA TCTTAAGAGC 
CGGGTCCGGT CCCTCTCAGT TACAAGTGAT TATTCATCTA GTCTGGAGAG ACCAAGAAAG 
CTGAAGGCCA TTGGCACCCT TGAGGGATCC TCACCTCACA GTGTCACTGA CGAGGATGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TGCACCCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG AGTGTCTCAG CTGGAGGCCC AGCTCCCGAA AACTGGAGTG 
GAAGGGAAGC TAGCAGAGGA GCTGAGATCA GCCTCATGGC CTGGGAAATA TGATTCCCTA 
ATTCAGGATC AGGCCCGAGA GCTGTCGTAC CTACGCCAGA AGATACGAGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCATGCAAAA GATACAGTAA AATCTTTTGA GGACCTTCTT 
AGGAGCAATG ACATTGACTA CTACCTGGGC CAGAGCTTCC GTGAGCAACT TGCCCAGGGA 
AGCCAGCTGA CAGAGAGACT CACCAGTAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTTGAACC ACTGGCCCTC AGGCTCAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATTG AAGTCCTGCA GGCCAAGCTA GATGCTCGGT CTCTCACACC CTCCAGCAGC 
CATGCATTGT CAGACTCCCA CCGCTCTCCC AGCAGCTCGT CCTTCCTGTC TGATGAGCTG 
GAAGCCTGCT CTGACATGGA TGTAGCCAGC GAGTACACAC ATTATGAAGA GAAGAAGGCT 
CCACCTGGTC ACTCAGATTC CATCCATCAT TTGAGTCATT CTGCTGTACT GTCTTCTAAA 
CCATCAGCAA CCAGTGTACC TCGGGGGGTT AAGGCCGAAC CCAGCAGCAA CCCCATCAGC 
TTGCCAGCTC CCCAGAACCC CCCCCAAGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTT 
CCCTCCATAT CCACACTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCCAC TGGCCCTCCC CTCCTTGGCT GCTGTGAGAC ACCTATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCAGA AACAATTGGG AGAAAGTGTC 
AGCACCGTCC ATCCTGCTTC CCCAGCTGCA TTGCCAAGCA ACCATTTAGA AGCCAACTCT 
TCCCAATATC TCAACCCTGC CCAGCCCCAC TCTCCTCCAA CTGGCAGCAC AGAATTGGGC 
AGAATCCTGG AGCCTGGCTA CCTGGGTAGC GGCAGCCAGT GGGATGTGAT GAGGCCTCAG 
AAGGGGAGTG TATCAGGCGA CTTATCCTCA GGATCCTCTG TGTGTCAGCT TAACTCCAAA 
CCCACAGGGG CTGACCTGCT AGAAGAACAT CTTGGTGAAA TCCGGAACCT GCGCCAGCGC 
CTAGAGGAGT CCATCTGCAT CAATGACCGC CTGCGGGAAC AGCTGGAACA CCGGCTCAGC 
TGCACAGCAC GTGGCAATGG ATCCACCTCT AATTTCTACA GTCCTGGCCT GGAACCCACA 
CCTCAGCTCT ACAATGAGAA CAGAGTCCTT AAGGAAGAAA ACCGGAGCCT TCAGGCTCAG 
CTGAGTCATG TTTCTAGAGA GCGCTCCCAG GAGACAGAAT GCCTGAGGGA GGCTCTACTG 
TCCTCTCAAT CCCGCCTTCA AGAGCTGGAA ATGGAGCTAG AGCACCAAAA GGAGGAACGG 
CAGCAGCTTT TGGAAGACTT GAAGGAGAAG CAGCAGGAGA TCTTGCACTT CCAGGAGGAG 
CGCCTGTCCC TCCAGGAGAA AGACTCTAGG CTGCAGCGTA AGCTGGCCCT ACTGCAGCAA 
CAGTGTGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCGCTTTATG GCAGTTCCAA GAAGGGTCTG AAAGCTTCCA CTTGGGATGC CTATCACCAG 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAAA GCATACAGGT GAATAACTGT CTGCGACTGC AGCTGGAGCA GCAGCTGGAT 
GGCGGAAGGA GCAAAACCAG CCTCAGTCCC TCTTCCACCA GCCAGGAGGA CCCTGGAGAC 
AAGCAAGACT CAATTGCTTC CCCTCCAGTC CGAGATGTTG GCATGAATTC TCCAGCTCTC 
ATGTTCCCTA GCTCTTCTCC TGCTGCTCCA GGCTCAGAGA CAGCTATTTT CAATAGGATA 
AATGAGCTGA ATTTGGATGC TTTTCCAGTA ATGAAGAACT CTCCCAAGCT GGAGGGCGAA 
GCTATCGATG GCTCCTTTGC CAATAAGCAC GGCCGCCATG TGATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGACAGCA GATTGGTGAG GGCAAAGTGC TGGTCAAAAA GATAACAGCA 
CTTGTGAGAT CAGCCTACAC CCTCCCTGGC CTTGAAACTC AGGCGACAGA GGTGCCAGGT 
GGCAAAGGCA TCCATGAACT TGGGAGCAGC ACCCAAGCCC TGCAGCACGT GCTGGAGGAG 
TCGGCCTCCC TCCTCAATAT GTTCTGGCGA GCGGCCCTGC CAAGCTCCAG CCCTGCCCTG 
TCTGGCAACA TGGGACAGGC CATGAAAAAG GAACTTCTGG AACTGAGAAC CAAACTGACC 
AAACAGGAGA CCCTCCTTCA GTGCACAGCT GAGCGTCTAA AGACCACCCA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAT TTGACCAGGA CACATGATGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGGT GAAGTCCCTA AGGGCTCTGG CAAGCACCCC AGTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976076.1
CDS	220..7239
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120601.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120601.2

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CATCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA TTTCCTGGAG GAACGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGAGGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGG 
GAACTCCAGG ACAAGAAACA GCATCTAGAT AAAACGTGGG CTGATGTGGA GAATCTCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGTCAG TTTGAAGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAGG AGGAATCCAG GCTAGCAAAG 
AATGAGGCTG CACGGATGGC AGCTCTGGTG GAAGCTGAGA AAGAATGTAA CCTGGAGCTA 
TCAGAGAAAC TGAAGGGAGT TGCCAATGAC AGGGAAGAGG TACCAATGGA CCAGGTCAAT 
CCTGACCAAT ACACTGAGGC CCTGGCCCAG CGGGACAAAA GAATTGAAGA ACTTCATCAA 
AGCCTGGCTT CCCAGGAGAA GCTTGTAGAA CAGCTGTCTC AAGAGAAACA ACAACTGCTC 
CATCTGTTGG AGGAACCAAT GAGCATGGAA ATACAGCCTG TGACTGAAGA GTTACTCAAG 
CAACAAAAGC TGGATTCAGA TGAGACCATT ATAACTCAGC AGTCTGTATC TGATTCCCAC 
TTGGCTGAAC TCCAGGAAAA AATCCAGCAA ATGGAGGCCA CCAACAAAAT TCTTCAAGAG 
AAACTGAATG AAATGAGCTG TGAGCTAAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GGTACAATTC AAAGCCTCAA GGAAACTCTG AAAAGTAGGG AAAATGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAATGACACA ATGGCAAAGC TTCGAGAAAT GCTACACCAA 
AGCCAGCTTG GACAACTTCA TGTATTTGAG GGTCACATAG GACAGGAGAG CTCACAGGGT 
ACTTCCCCAG CTCAGCAACA GATGGCTCTG CTTGATCTTC AGAGTGGTTT GTTCTACAGC 
CAGCTTGAGA TTCAGAAACT CCAGAGGTTG GTACGTCAAA AAGAACGCCA ACTGGCTGAT 
GCCAAACGAT GTGTCCAATT TGTAGAGGCT GCAGCCCATG AAAGAGAACA GCAGAAAGAG 
GCATCTTGGA AACATAATCA GGAATTACGG AAAGCCTTGC AGCAGCTCCA AGGAGAATTA 
GAGAGTAAGA GCCAACAGCT TCGTACCCTG GAGACTGAAA AATGTAATGA GATCAGAGCC 
CATGAGCAAC ACATTCAACA CCTAAACCAT AGTCTGAGCC ACAAAGAGCA GCTGCTTCAG 
GAATTTCGGG AGCTCCTGCA GTATCGAGAT AAGTCAGACA AAACCCTTGA AGCAAATGAA 
ATGTTGCTTG AGAAACTTCG GCAGCGAATA CAAGACCGAG ATGTTGCTTT GGAGCGGGCT 
ATAGATGAAA AGTTCTCCGC CCTGGAAGAG AAAGAAAAAG AACTGCGTCA ATTTCATCTT 
GCTGTGCGAG AGCGTGATCA TGACTTAGAG AGATTGCGTG CTATTCTCTC CTCTAATGAA 
GCTACCATGC AGAGTATGGA GAGTCTCCTG AGGGCCAAAG GCCTAGAAGT AGAGCAGTTA 
TCAGCTACCT GTCAAAACCT CCAGTGGCTG AAAGAAGAAA AGGAAACCAA ATTTAGCCAT 
TGGCAGAAGG AACAAGAAAG TATCATTCAG CAGTTACAGA CATCTCTGCA TGACAGGAAC 
AAAGAAGTGG AGGATCTGAG TGCCACCTTG TTCTGTAAGC TTGGACCTGG GCAGAGTGAG 
GTAGCAGAAG AGCTGTGCCA GCGCCTACAG CGAAAGGAAA GGATGCTACA GGAACTGCTA 
ACCGATCGAA ACAAACAAGC AGTGGAACAC GAAATAGAGA TTCAAGGCCT GCTTCAGTCT 
ATGAGCACCA GGGAGCAGGA GAGCCAAGCC GCTGCAGGGA AGATGGTGCA AGCCCTTATG 
GAAAGAAATT CAGAACTACA GGCCCTGCGC CAGTATTTAG GAGGTAAAGA CTTCATGGTT 
TCCCAAGCAC TCATCTCTAA CCAACCCACA GAAGTCACCT CCAACAGCCC TCGTCTTGGA 
GAGCAGACTG ATCAAGGTTC AGTGCATATA CCCTCCAAAG ATGATAGCAT TTCCCTGACT 
GCCAAAGGGG ATACCAGTAT ACCCAGGTCC TCATTAGGAG ACCAGGATAC AGTTGCAGGG 
CTGGAAAAAG AACTGAGTAA TGCCAAAGAG GAGCTTGAGC TCATGGCTAA GAAAGAACGA 
GAAAGTCGGA TGGAACTTTC TGCACTACAG TCCATGGTGG CCGTGCAAGA GGAAGAGCTG 
CAGGTGCAGG CTGCTGACAT GGAATCCCTG ACCAGGAATA TACAGATTAA AGAAGACCTC 
ATAAAGGACC TGCAGATGCA ACTGGTTGAT CCTGAAGATA TACCAGCTAT GGAACGCCTG 
ACCCAAGAGG TCTTACTTCT TCGGGAAAAA GTTGCTTCAG TAGAATCACA GGGTCAGGAA 
ACGTTAGGAA ATCGAAGACA ACAATTGCTG CTAATGCTCG AAGGGCTGGT GGATGAACGG 
AGTCGGCTCA ATGAAGCTTT ACAAGCAGAG AGACAGCTGT ACAGCAGCCT GGTGAAGTTC 
CATGCCCATC CAGAGAGCTC TGAGAGAGAC CGAACTCTGC AGGTGGAACT AGAAGGGGCC 
CAGGTGATAC GTGGTCGGCT AGAAGAAGTT CTTGGAAGAA GCCTGGAGCG CTTAAGCAGG 
CTGGAGACCC TGGCTGCCAT TGGAGGTGCA GCTGCAGGGG AAGATACAGA AGATGCTAGC 
ACTGAGTTCA CGGACAGCAT CGAGGAGGAG GCTGCACACC ATAATCACCA GCAACTCATC 
AAGGTGGCTT TGGAGAAAAG TCTGGCAACT GTGGAGACTC AGAACGTATG TCTCCCCCCT 
CCTTCCTCAG CAGGAGGGGA CAATAACAGG GGTCTTCAGG AGGAGATGCT CCACCTGAGG 
GCTGAGATCC ACCAGCACTT AGAGGAGAAG AGGAGAGCTG AGGGGGAACT GAAGGAGCTA 
AAGGCTCAAA TTGAGGAAGC AGGATTCTCC TCTGTGGCCC ACATCAGGAA CACCATGCTG 
AGCCTTTGCC TTGAGAATGC AGAGCTGAAA GAGCAGATGG GAGAAGCAAT GTCTGATGGA 
TGGGAGATCG AGGAAGACAA GGAGAAGGGC GAGGTGACAG TGGAAACTGT GGCAGCCAAA 
GGAGGTCTGA ATGAAAATAG CCTTCAGGCT GAGTTCAGAA AGCTCCAGGG AAAACTGAAG 
CATGCCCACA ACATTATCAA CCTCCTCAAA GAGCAGCTGG TGCTGAGCAG CAAGGATGGG 
AATAGTAAAC TTAGTCCAGA GCTCCTAGTG CGCCTGGACC GGGAGATCGA CAGAATGAAC 
ACAGAGCTGG TCTGTTCTCC GGAGAAGCCC CAGTGCCAGG AAGAAGAGCA TATGACCACA 
AGGCCCTGCC CCAGACCCCA GAGCCTTGAC CTTGGGGCTA CCCTCTCAGT GGATGGCCAC 
CAACTGGATA ACCGGCCCCA GGCCCAAGAC CCTGGGCCTC AGTCAGAATT TAGCCTTCCT 
GGATCCACCA AGCACCTGCG CTCCCAGCTG GCCCAATGCA GACAACGCTA TCAGGATCTC 
CAGGAGAAGC TGCTGATATC GGAAGCCACA GTCTTTGCAC AGGCCAACCA ACTGGAGAAA 
TACCGAGTCA TGCTTAGTGA ATCCTTGGTG AAGCAGGACA GCAAGCAGGT GCAGGTGGAC 
CTCCAGGATC TGGGCTATGA AACCTGTGGC CGAAGTGAGA ATGAGGCTGA GCGGGAGGAG 
AGCACCAGTC CCGAGTGTGA GGAGCCTGAC AGCCTCAGGG AAACAGTCCT GATGGTGGGG 
CACCTGGGCT CACTGCTGGC CAGCCCTCCA GGGAAGAAGC CCTTGGAAAG CCATCGGGGG 
AAGCAGGAGG AGTTCCAGGC ATATGGAAAG TCAGAAGACA TCTCTGTCCT ACATAAGGAC 
ATCAGAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGA TCATTCAGAA TCTTAAGAGC 
CGGGTCCGGT CCCTCTCAGT TACAAGTGAT TATTCATCTA GTCTGGAGAG ACCAAGAAAG 
CTGAAGGCCA TTGGCACCCT TGAGGGATCC TCACCTCACA GTGTCACTGA CGAGGATGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TGCACCCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG AGTGTCTCAG CTGGAGGCCC AGCTCCCGAA AACTGGAGTG 
GAAGGGAAGC TAGCAGAGGA GCTGAGATCA GCCTCATGGC CTGGGAAATA TGATTCCCTA 
ATTCAGGATC AGGCCCGAGA GCTGTCGTAC CTACGCCAGA AGATACGAGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCATGCAAAA GATACAGTAA AATCTTTTGA GGACCTTCTT 
AGGAGCAATG ACATTGACTA CTACCTGGGC CAGAGCTTCC GTGAGCAACT TGCCCAGGGA 
AGCCAGCTGA CAGAGAGACT CACCAGTAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTTGAACC ACTGGCCCTC AGGCTCAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATTG AAGTCCTGCA GGCCAAGCTA GATGCTCGGT CTCTCACACC CTCCAGCAGC 
CATGCATTGT CAGACTCCCA CCGCTCTCCC AGCAGCTCGT CCTTCCTGTC TGATGAGCTG 
GAAGCCTGCT CTGACATGGA TGTAGCCAGC GAGTACACAC ATTATGAAGA GAAGAAGGCT 
CCACCTGGTC ACTCAGATTC CATCCATCAT TTGAGTCATT CTGCTGTACT GTCTTCTAAA 
CCATCAGCAA CCAGTGTACC TCGGGGGGTT AAGGCCGAAC CCAGCAGCAA CCCCATCAGC 
TTGCCAGCTC CCCAGAACCC CCCCCAAGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTT 
CCCTCCATAT CCACACTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCCAC TGGCCCTCCC CTCCTTGGCT GCTGTGAGAC ACCTATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCAGA AACAATTGGG AGAAAGTGTC 
AGCACCGTCC ATCCTGCTTC CCCAGCTGCA TTGCCAAGCA ACCATTTAGA AGCCAACTCT 
TCCCAATATC TCAACCCTGC CCAGCCCCAC TCTCCTCCAA CTGGCAGCAC AGAATTGGGC 
AGAATCCTGG AGCCTGGCTA CCTGGGTAGC GGCAGCCAGT GGGATGTGAT GAGGCCTCAG 
AAGGGGAGTG TATCAGGCGA CTTATCCTCA GGATCCTCTG TGTGTCAGCT TAACTCCAAA 
CCCACAGGGG CTGACCTGCT AGAAGAACAT CTTGGTGAAA TCCGGAACCT GCGCCAGCGC 
CTAGAGGAGT CCATCTGCAT CAATGACCGC CTGCGGGAAC AGCTGGAACA CCGGCTCAGC 
TGCACAGCAC GTGGCAATGG ATCCACCTCT AATTTCTACA GTCCTGGCCT GGAACCCACA 
CCTCAGCTCT ACAATGAGAA CAGAGTCCTT AAGGAAGAAA ACCGGAGCCT TCAGGCTCAG 
CTGAGTCATG TTTCTAGAGA GCGCTCCCAG GAGACAGAAT GCCTGAGGGA GGCTCTACTG 
TCCTCTCAAT CCCGCCTTCA AGAGCTGGAA ATGGAGCTAG AGCACCAAAA GGAGGAACGG 
CAGCAGCTTT TGGAAGACTT GAAGGAGAAG CAGCAGGAGA TCTTGCACTT CCAGGAGGAG 
CGCCTGTCCC TCCAGGAGAA AGACTCTAGG CTGCAGCGTA AGCTGGCCCT ACTGCAGCAA 
CAGTGTGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCGCTTTATG GCAGTTCCAA GAAGGGTCTG AAAGCTTCCA CTTGGGATGC CTATCACCAG 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAAA GCATACAGGT GAATAACTGT CTGCGACTGC AGCTGGAGCA GCAGCTGGAT 
GGCGGAAGGA GCAAAACCAG CCTCAGTCCC TCTTCCACCA GCCAGGAGGA CCCTGGAGAC 
AAGCAAGACT CAATTGCTTC CCCTCCAGTC CGAGATGTTG GCATGAATTC TCCAGCTCTC 
ATGTTCCCTA GCTCTTCTCC TGCTGCTCCA GGCTCAGAGA CAGCTATTTT CAATAGGATA 
AATGAGCTGA ATTTGGATGC TTTTCCAGTA ATGAAGAACT CTCCCAAGCT GGAGGGCGAA 
GCTATCGATG GCTCCTTTGC CAATAAGCAC GGCCGCCATG TGATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGACAGCA GATTGGTGAG GGCAAAGTGC TGGTCAAAAA GATAACAGCA 
CTTGTGAGAT CAGCCTACAC CCTCCCTGGC CTTGAAACTC AGGCGACAGA GGTGCCAGGT 
GGCAAAGGCA TCCATGAACT TGGGAGCAGC ACCCAAGCCC TGCAGCACGT GCTGGAGGAG 
TCGGCCTCCC TCCTCAATAT GTTCTGGCGA GCGGCCCTGC CAAGCTCCAG CCCTGCCCTG 
TCTGGCAACA TGGGACAGGC CATGAAAAAG GAACTTCTGG AACTGAGAAC CAAACTGACC 
AAACAGGAGA CCCTCCTTCA GTGCACAGCT GAGCGTCTAA AGACCACCCA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAT TTGACCAGGA CACATGATGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGGT GAAGTCCCTA AGGGCTCTGG CAAGCACCCC AGTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630690.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630747.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X4, mRNA.

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CloneID	OCe19357
Clone ID Related Accession (Same CDS sequence)	XM_005630690.2 , XM_005630690.3
Accession Version	XM_005630690.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA GCTGCAGGAT CTGTGCCCGG GAGCTGTGTG GGAACCAGCG GCGCTGGATC 
TTCCACACGG CATCTAAACT CAACCTCCAG GTTCTGCTCT CGCACGTCCT GGGCAAGGAT 
GTGCCCCGCG ATGGCAAAGC CGAGTTTGCT TGCAGCAAAT GTGCTTTCAT GCTTGATCGG 
ATCTATAGAT TCGACACCGT TATTGCACGG ATCGAAGCTC TTTCTATCGA GCGTTTGCAA 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGAGGACT CTGGCACGGA AACCAAGGCG GGGAATGGGA CCGTTGACAT TTCCGGCTTA 
CCCGACGTAA GATACTCTGC GCTGCTCCAG GAGGACTTTG CCTATTCAGG GTTTGAGTGT 
TGGGTAGAAA ACGAGGATCA GATTCAGGAG CTACACAGCT GCCATACTTC GGAAGGTCCT 
GGAAACCGAC CCAGGAGATG CCGTGGTTGT GCAGCCTTGC GGGTTGCTGA TTCTGACTAT 
GAAGCCATTT GTAAGGTGCC CCGAAAGGTG GCCAGAAGTA TCTCGTGTGC TCCTTCTAGG 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCATTGTCTG AGGTTGGGCC ACCGGATTTA 
CCAAGTACAA AGGTACCCCC AGATGGAGAG AGCATGGAGG AAGGGACACC CGGATCCTCT 
GTGGAATCTT TGGATGCAAG TGTCCAGGCT AGTCCTCCAC AACAAAAGGA TGAGGAAACG 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGTTGTT CAGATGAACA GGCTCCACAG 
CATGTGTGTA ACCACAAGCT AGAGCTAGCT CTTAACATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GTCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAG CCCACCTGGA 
GCCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTCTCCTCAA CAGGTCTTTG 
AAACCCCTTT ACAAGATACC TGTGAATTAT CCCTTGGAGC TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TTTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCTGTGAC TGAAGAGTTA 
CTCAAGCAAC AAAAGCTGGA TTCAGATGAG ACCATTATAA CTCAGCAGTC TGTATCTGAT 
TCCCACTTGG CTGAACTCCA GGAAAAAATC CAGCAAATGG AGGCCACCAA CAAAATTCTT 
CAAGAGAAAC TGAATGAAAT GAGCTGTGAG CTAAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGGTA CAATTCAAAG CCTCAAGGAA ACTCTGAAAA GTAGGGAAAA TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAT GACACAATGG CAAAGCTTCG AGAAATGCTA 
CACCAAAGCC AGCTTGGACA ACTTCATGTA TTTGAGGGTC ACATAGGACA GGAGAGCTCA 
CAGGGTACTT CCCCAGCTCA GCAACAGATG GCTCTGCTTG ATCTTCAGAG TGGTTTGTTC 
TACAGCCAGC TTGAGATTCA GAAACTCCAG AGGTTGGTAC GTCAAAAAGA ACGCCAACTG 
GCTGATGCCA AACGATGTGT CCAATTTGTA GAGGCTGCAG CCCATGAAAG AGAACAGCAG 
AAAGAGGCAT CTTGGAAACA TAATCAGGAA TTACGGAAAG CCTTGCAGCA GCTCCAAGGA 
GAATTAGAGA GTAAGAGCCA ACAGCTTCGT ACCCTGGAGA CTGAAAAATG TAATGAGATC 
AGAGCCCATG AGCAACACAT TCAACACCTA AACCATAGTC TGAGCCACAA AGAGCAGCTG 
CTTCAGGAAT TTCGGGAGCT CCTGCAGTAT CGAGATAAGT CAGACAAAAC CCTTGAAGCA 
AATGAAATGT TGCTTGAGAA ACTTCGGCAG CGAATACAAG ACCGAGATGT TGCTTTGGAG 
CGGGCTATAG ATGAAAAGTT CTCCGCCCTG GAAGAGAAAG AAAAAGAACT GCGTCAATTT 
CATCTTGCTG TGCGAGAGCG TGATCATGAC TTAGAGAGAT TGCGTGCTAT TCTCTCCTCT 
AATGAAGCTA CCATGCAGAG TATGGAGAGT CTCCTGAGGG CCAAAGGCCT AGAAGTAGAG 
CAGTTATCAG CTACCTGTCA AAACCTCCAG TGGCTGAAAG AAGAAAAGGA AACCAAATTT 
AGCCATTGGC AGAAGGAACA AGAAAGTATC ATTCAGCAGT TACAGACATC TCTGCATGAC 
AGGAACAAAG AAGTGGAGGA TCTGAGTGCC ACCTTGTTCT GTAAGCTTGG ACCTGGGCAG 
AGTGAGGTAG CAGAAGAGCT GTGCCAGCGC CTACAGCGAA AGGAAAGGAT GCTACAGGAA 
CTGCTAACCG ATCGAAACAA ACAAGCAGTG GAACACGAAA TAGAGATTCA AGGCCTGCTT 
CAGTCTATGA GCACCAGGGA GCAGGAGAGC CAAGCCGCTG CAGGGAAGAT GGTGCAAGCC 
CTTATGGAAA GAAATTCAGA ACTACAGGCC CTGCGCCAGT ATTTAGGAGG TAAAGACTTC 
ATGGTTTCCC AAGCACTCAT CTCTAACCAA CCCACAGAAG TCACCTCCAA CAGCCCTCGT 
CTTGGAGAGC AGACTGATCA AGGTTCAGTG CATATACCCT CCAAAGATGA TAGCATTTCC 
CTGACTGCCA AAGGGGATAC CAGTATACCC AGGTCCTCAT TAGGAGACCA GGATACAGTT 
GCAGGGCTGG AAAAAGAACT GAGTAATGCC AAAGAGGAGC TTGAGCTCAT GGCTAAGAAA 
GAACGAGAAA GTCGGATGGA ACTTTCTGCA CTACAGTCCA TGGTGGCCGT GCAAGAGGAA 
GAGCTGCAGG TGCAGGCTGC TGACATGGAA TCCCTGACCA GGAATATACA GATTAAAGAA 
GACCTCATAA AGGACCTGCA GATGCAACTG GTTGATCCTG AAGATATACC AGCTATGGAA 
CGCCTGACCC AAGAGGTCTT ACTTCTTCGG GAAAAAGTTG CTTCAGTAGA ATCACAGGGT 
CAGGAAACGT TAGGAAATCG AAGACAACAA TTGCTGCTAA TGCTCGAAGG GCTGGTGGAT 
GAACGGAGTC GGCTCAATGA AGCTTTACAA GCAGAGAGAC AGCTGTACAG CAGCCTGGTG 
AAGTTCCATG CCCATCCAGA GAGCTCTGAG AGAGACCGAA CTCTGCAGGT GGAACTAGAA 
GGGGCCCAGG TGATACGTGG TCGGCTAGAA GAAGTTCTTG GAAGAAGCCT GGAGCGCTTA 
AGCAGGCTGG AGACCCTGGC TGCCATTGGA GGTGCAGCTG CAGGGGAAGA TACAGAAGAT 
GCTAGCACTG AGTTCACGGA CAGCATCGAG GAGGAGGCTG CACACCATAA TCACCAGCAA 
CTCATCAAGG TGGCTTTGGA GAAAAGTCTG GCAACTGTGG AGACTCAGAA CGTATGTCTC 
CCCCCTCCTT CCTCAGCAGG AGGGGACAAT AACAGGGGTC TTCAGGAGGA GATGCTCCAC 
CTGAGGGCTG AGATCCACCA GCACTTAGAG GAGAAGAGGA GAGCTGAGGG GGAACTGAAG 
GAGCTAAAGG CTCAAATTGA GGAAGCAGGA TTCTCCTCTG TGGCCCACAT CAGGAACACC 
ATGCTGAGCC TTTGCCTTGA GAATGCAGAG CTGAAAGAGC AGATGGGAGA AGCAATGTCT 
GATGGATGGG AGATCGAGGA AGACAAGGAG AAGGGCGAGG TGACAGTGGA AACTGTGGCA 
GCCAAAGGAG GTCTGAATGA AAATAGCCTT CAGGCTGAGT TCAGAAAGCT CCAGGGAAAA 
CTGAAGCATG CCCACAACAT TATCAACCTC CTCAAAGAGC AGCTGGTGCT GAGCAGCAAG 
GATGGGAATA GTAAACTTAG TCCAGAGCTC CTAGTGCGCC TGGACCGGGA GATCGACAGA 
ATGAACACAG AGCTGGTCTG TTCTCCGGAG AAGCCCCAGT GCCAGGAAGA AGAGCATATG 
ACCACAAGGC CCTGCCCCAG ACCCCAGAGC CTTGACCTTG GGGCTACCCT CTCAGTGGAT 
GGCCACCAAC TGGATAACCG GCCCCAGGCC CAAGACCCTG GGCCTCAGTC AGAATTTAGC 
CTTCCTGGAT CCACCAAGCA CCTGCGCTCC CAGCTGGCCC AATGCAGACA ACGCTATCAG 
GATCTCCAGG AGAAGCTGCT GATATCGGAA GCCACAGTCT TTGCACAGGC CAACCAACTG 
GAGAAATACC GAGTCATGCT TAGTGAATCC TTGGTGAAGC AGGACAGCAA GCAGGTGCAG 
GTGGACCTCC AGGATCTGGG CTATGAAACC TGTGGCCGAA GTGAGAATGA GGCTGAGCGG 
GAGGAGAGCA CCAGTCCCGA GTGTGAGGAG CCTGACAGCC TCAGGGAAAC AGTCCTGATG 
GTGGGGCACC TGGGCTCACT GCTGGCCAGC CCTCCAGGGA AGAAGCCCTT GGAAAGCCAT 
CGGGGGAAGC AGGAGGAGTT CCAGGCATAT GGAAAGTCAG AAGACATCTC TGTCCTACAT 
AAGGACATCA GAGATCTGAA GGCCCAGCTG CAGAATGCCA ACAAGATCAT TCAGAATCTT 
AAGAGCCGGG TCCGGTCCCT CTCAGTTACA AGTGATTATT CATCTAGTCT GGAGAGACCA 
AGAAAGCTGA AGGCCATTGG CACCCTTGAG GGATCCTCAC CTCACAGTGT CACTGACGAG 
GATGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTGCA CCCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGAGTG TCTCAGCTGG AGGCCCAGCT CCCGAAAACT 
GGAGTGGAAG GGAAGCTAGC AGAGGAGCTG AGATCAGCCT CATGGCCTGG GAAATATGAT 
TCCCTAATTC AGGATCAGGC CCGAGAGCTG TCGTACCTAC GCCAGAAGAT ACGAGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAT GCAAAAGATA CAGTAAAATC TTTTGAGGAC 
CTTCTTAGGA GCAATGACAT TGACTACTAC CTGGGCCAGA GCTTCCGTGA GCAACTTGCC 
CAGGGAAGCC AGCTGACAGA GAGACTCACC AGTAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT TGAACCACTG GCCCTCAGGC TCAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATTGAAGT CCTGCAGGCC AAGCTAGATG CTCGGTCTCT CACACCCTCC 
AGCAGCCATG CATTGTCAGA CTCCCACCGC TCTCCCAGCA GCTCGTCCTT CCTGTCTGAT 
GAGCTGGAAG CCTGCTCTGA CATGGATGTA GCCAGCGAGT ACACACATTA TGAAGAGAAG 
AAGGCTCCAC CTGGTCACTC AGATTCCATC CATCATTTGA GTCATTCTGC TGTACTGTCT 
TCTAAACCAT CAGCAACCAG TGTACCTCGG GGGGTTAAGG CCGAACCCAG CAGCAACCCC 
ATCAGCTTGC CAGCTCCCCA GAACCCCCCC CAAGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTTCCCT CCATATCCAC ACTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCCACTGGC CCTCCCCTCC TTGGCTGCTG TGAGACACCT 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCAGAAACA ATTGGGAGAA 
AGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630747.1
CDS	429..8165
Translation	MKESCRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVPRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNEDSGTETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQELHSCHTSEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVARSISCAPSRWSTSICTEEPALSEVGPPDLPSTKVPPDGESMEEGTPGSSVESLDASVQASPPQQKDEETERSAKELAKCDCCSDEQAPQHVCNHKLELALNMIKGLDYKPIQSPRGSKLPIPVKSSPPGARPGHIMTDGVSSGLLNRSLKPLYKIPVNYPLELSDLQELWDDLCEDYLPLRVQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630690.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630690.2

ORF Insert Sequence:

ATGAAGGAGA GCTGCAGGAT CTGTGCCCGG GAGCTGTGTG GGAACCAGCG GCGCTGGATC 
TTCCACACGG CATCTAAACT CAACCTCCAG GTTCTGCTCT CGCACGTCCT GGGCAAGGAT 
GTGCCCCGCG ATGGCAAAGC CGAGTTTGCT TGCAGCAAAT GTGCTTTCAT GCTTGATCGG 
ATCTATAGAT TCGACACCGT TATTGCACGG ATCGAAGCTC TTTCTATCGA GCGTTTGCAA 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGAGGACT CTGGCACGGA AACCAAGGCG GGGAATGGGA CCGTTGACAT TTCCGGCTTA 
CCCGACGTAA GATACTCTGC GCTGCTCCAG GAGGACTTTG CCTATTCAGG GTTTGAGTGT 
TGGGTAGAAA ACGAGGATCA GATTCAGGAG CTACACAGCT GCCATACTTC GGAAGGTCCT 
GGAAACCGAC CCAGGAGATG CCGTGGTTGT GCAGCCTTGC GGGTTGCTGA TTCTGACTAT 
GAAGCCATTT GTAAGGTGCC CCGAAAGGTG GCCAGAAGTA TCTCGTGTGC TCCTTCTAGG 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCATTGTCTG AGGTTGGGCC ACCGGATTTA 
CCAAGTACAA AGGTACCCCC AGATGGAGAG AGCATGGAGG AAGGGACACC CGGATCCTCT 
GTGGAATCTT TGGATGCAAG TGTCCAGGCT AGTCCTCCAC AACAAAAGGA TGAGGAAACG 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGTTGTT CAGATGAACA GGCTCCACAG 
CATGTGTGTA ACCACAAGCT AGAGCTAGCT CTTAACATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GTCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAG CCCACCTGGA 
GCCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTCTCCTCAA CAGGTCTTTG 
AAACCCCTTT ACAAGATACC TGTGAATTAT CCCTTGGAGC TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TTTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCTGTGAC TGAAGAGTTA 
CTCAAGCAAC AAAAGCTGGA TTCAGATGAG ACCATTATAA CTCAGCAGTC TGTATCTGAT 
TCCCACTTGG CTGAACTCCA GGAAAAAATC CAGCAAATGG AGGCCACCAA CAAAATTCTT 
CAAGAGAAAC TGAATGAAAT GAGCTGTGAG CTAAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGGTA CAATTCAAAG CCTCAAGGAA ACTCTGAAAA GTAGGGAAAA TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAT GACACAATGG CAAAGCTTCG AGAAATGCTA 
CACCAAAGCC AGCTTGGACA ACTTCATGTA TTTGAGGGTC ACATAGGACA GGAGAGCTCA 
CAGGGTACTT CCCCAGCTCA GCAACAGATG GCTCTGCTTG ATCTTCAGAG TGGTTTGTTC 
TACAGCCAGC TTGAGATTCA GAAACTCCAG AGGTTGGTAC GTCAAAAAGA ACGCCAACTG 
GCTGATGCCA AACGATGTGT CCAATTTGTA GAGGCTGCAG CCCATGAAAG AGAACAGCAG 
AAAGAGGCAT CTTGGAAACA TAATCAGGAA TTACGGAAAG CCTTGCAGCA GCTCCAAGGA 
GAATTAGAGA GTAAGAGCCA ACAGCTTCGT ACCCTGGAGA CTGAAAAATG TAATGAGATC 
AGAGCCCATG AGCAACACAT TCAACACCTA AACCATAGTC TGAGCCACAA AGAGCAGCTG 
CTTCAGGAAT TTCGGGAGCT CCTGCAGTAT CGAGATAAGT CAGACAAAAC CCTTGAAGCA 
AATGAAATGT TGCTTGAGAA ACTTCGGCAG CGAATACAAG ACCGAGATGT TGCTTTGGAG 
CGGGCTATAG ATGAAAAGTT CTCCGCCCTG GAAGAGAAAG AAAAAGAACT GCGTCAATTT 
CATCTTGCTG TGCGAGAGCG TGATCATGAC TTAGAGAGAT TGCGTGCTAT TCTCTCCTCT 
AATGAAGCTA CCATGCAGAG TATGGAGAGT CTCCTGAGGG CCAAAGGCCT AGAAGTAGAG 
CAGTTATCAG CTACCTGTCA AAACCTCCAG TGGCTGAAAG AAGAAAAGGA AACCAAATTT 
AGCCATTGGC AGAAGGAACA AGAAAGTATC ATTCAGCAGT TACAGACATC TCTGCATGAC 
AGGAACAAAG AAGTGGAGGA TCTGAGTGCC ACCTTGTTCT GTAAGCTTGG ACCTGGGCAG 
AGTGAGGTAG CAGAAGAGCT GTGCCAGCGC CTACAGCGAA AGGAAAGGAT GCTACAGGAA 
CTGCTAACCG ATCGAAACAA ACAAGCAGTG GAACACGAAA TAGAGATTCA AGGCCTGCTT 
CAGTCTATGA GCACCAGGGA GCAGGAGAGC CAAGCCGCTG CAGGGAAGAT GGTGCAAGCC 
CTTATGGAAA GAAATTCAGA ACTACAGGCC CTGCGCCAGT ATTTAGGAGG TAAAGACTTC 
ATGGTTTCCC AAGCACTCAT CTCTAACCAA CCCACAGAAG TCACCTCCAA CAGCCCTCGT 
CTTGGAGAGC AGACTGATCA AGGTTCAGTG CATATACCCT CCAAAGATGA TAGCATTTCC 
CTGACTGCCA AAGGGGATAC CAGTATACCC AGGTCCTCAT TAGGAGACCA GGATACAGTT 
GCAGGGCTGG AAAAAGAACT GAGTAATGCC AAAGAGGAGC TTGAGCTCAT GGCTAAGAAA 
GAACGAGAAA GTCGGATGGA ACTTTCTGCA CTACAGTCCA TGGTGGCCGT GCAAGAGGAA 
GAGCTGCAGG TGCAGGCTGC TGACATGGAA TCCCTGACCA GGAATATACA GATTAAAGAA 
GACCTCATAA AGGACCTGCA GATGCAACTG GTTGATCCTG AAGATATACC AGCTATGGAA 
CGCCTGACCC AAGAGGTCTT ACTTCTTCGG GAAAAAGTTG CTTCAGTAGA ATCACAGGGT 
CAGGAAACGT TAGGAAATCG AAGACAACAA TTGCTGCTAA TGCTCGAAGG GCTGGTGGAT 
GAACGGAGTC GGCTCAATGA AGCTTTACAA GCAGAGAGAC AGCTGTACAG CAGCCTGGTG 
AAGTTCCATG CCCATCCAGA GAGCTCTGAG AGAGACCGAA CTCTGCAGGT GGAACTAGAA 
GGGGCCCAGG TGATACGTGG TCGGCTAGAA GAAGTTCTTG GAAGAAGCCT GGAGCGCTTA 
AGCAGGCTGG AGACCCTGGC TGCCATTGGA GGTGCAGCTG CAGGGGAAGA TACAGAAGAT 
GCTAGCACTG AGTTCACGGA CAGCATCGAG GAGGAGGCTG CACACCATAA TCACCAGCAA 
CTCATCAAGG TGGCTTTGGA GAAAAGTCTG GCAACTGTGG AGACTCAGAA CGTATGTCTC 
CCCCCTCCTT CCTCAGCAGG AGGGGACAAT AACAGGGGTC TTCAGGAGGA GATGCTCCAC 
CTGAGGGCTG AGATCCACCA GCACTTAGAG GAGAAGAGGA GAGCTGAGGG GGAACTGAAG 
GAGCTAAAGG CTCAAATTGA GGAAGCAGGA TTCTCCTCTG TGGCCCACAT CAGGAACACC 
ATGCTGAGCC TTTGCCTTGA GAATGCAGAG CTGAAAGAGC AGATGGGAGA AGCAATGTCT 
GATGGATGGG AGATCGAGGA AGACAAGGAG AAGGGCGAGG TGACAGTGGA AACTGTGGCA 
GCCAAAGGAG GTCTGAATGA AAATAGCCTT CAGGCTGAGT TCAGAAAGCT CCAGGGAAAA 
CTGAAGCATG CCCACAACAT TATCAACCTC CTCAAAGAGC AGCTGGTGCT GAGCAGCAAG 
GATGGGAATA GTAAACTTAG TCCAGAGCTC CTAGTGCGCC TGGACCGGGA GATCGACAGA 
ATGAACACAG AGCTGGTCTG TTCTCCGGAG AAGCCCCAGT GCCAGGAAGA AGAGCATATG 
ACCACAAGGC CCTGCCCCAG ACCCCAGAGC CTTGACCTTG GGGCTACCCT CTCAGTGGAT 
GGCCACCAAC TGGATAACCG GCCCCAGGCC CAAGACCCTG GGCCTCAGTC AGAATTTAGC 
CTTCCTGGAT CCACCAAGCA CCTGCGCTCC CAGCTGGCCC AATGCAGACA ACGCTATCAG 
GATCTCCAGG AGAAGCTGCT GATATCGGAA GCCACAGTCT TTGCACAGGC CAACCAACTG 
GAGAAATACC GAGTCATGCT TAGTGAATCC TTGGTGAAGC AGGACAGCAA GCAGGTGCAG 
GTGGACCTCC AGGATCTGGG CTATGAAACC TGTGGCCGAA GTGAGAATGA GGCTGAGCGG 
GAGGAGAGCA CCAGTCCCGA GTGTGAGGAG CCTGACAGCC TCAGGGAAAC AGTCCTGATG 
GTGGGGCACC TGGGCTCACT GCTGGCCAGC CCTCCAGGGA AGAAGCCCTT GGAAAGCCAT 
CGGGGGAAGC AGGAGGAGTT CCAGGCATAT GGAAAGTCAG AAGACATCTC TGTCCTACAT 
AAGGACATCA GAGATCTGAA GGCCCAGCTG CAGAATGCCA ACAAGATCAT TCAGAATCTT 
AAGAGCCGGG TCCGGTCCCT CTCAGTTACA AGTGATTATT CATCTAGTCT GGAGAGACCA 
AGAAAGCTGA AGGCCATTGG CACCCTTGAG GGATCCTCAC CTCACAGTGT CACTGACGAG 
GATGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTGCA CCCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGAGTG TCTCAGCTGG AGGCCCAGCT CCCGAAAACT 
GGAGTGGAAG GGAAGCTAGC AGAGGAGCTG AGATCAGCCT CATGGCCTGG GAAATATGAT 
TCCCTAATTC AGGATCAGGC CCGAGAGCTG TCGTACCTAC GCCAGAAGAT ACGAGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAT GCAAAAGATA CAGTAAAATC TTTTGAGGAC 
CTTCTTAGGA GCAATGACAT TGACTACTAC CTGGGCCAGA GCTTCCGTGA GCAACTTGCC 
CAGGGAAGCC AGCTGACAGA GAGACTCACC AGTAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT TGAACCACTG GCCCTCAGGC TCAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATTGAAGT CCTGCAGGCC AAGCTAGATG CTCGGTCTCT CACACCCTCC 
AGCAGCCATG CATTGTCAGA CTCCCACCGC TCTCCCAGCA GCTCGTCCTT CCTGTCTGAT 
GAGCTGGAAG CCTGCTCTGA CATGGATGTA GCCAGCGAGT ACACACATTA TGAAGAGAAG 
AAGGCTCCAC CTGGTCACTC AGATTCCATC CATCATTTGA GTCATTCTGC TGTACTGTCT 
TCTAAACCAT CAGCAACCAG TGTACCTCGG GGGGTTAAGG CCGAACCCAG CAGCAACCCC 
ATCAGCTTGC CAGCTCCCCA GAACCCCCCC CAAGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTTCCCT CCATATCCAC ACTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCCACTGGC CCTCCCCTCC TTGGCTGCTG TGAGACACCT 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCAGAAACA ATTGGGAGAA 
AGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630690.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630747.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X4, mRNA.

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CloneID	OCe19357
Clone ID Related Accession (Same CDS sequence)	XM_005630690.2 , XM_005630690.3
Accession Version	XM_005630690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA GCTGCAGGAT CTGTGCCCGG GAGCTGTGTG GGAACCAGCG GCGCTGGATC 
TTCCACACGG CATCTAAACT CAACCTCCAG GTTCTGCTCT CGCACGTCCT GGGCAAGGAT 
GTGCCCCGCG ATGGCAAAGC CGAGTTTGCT TGCAGCAAAT GTGCTTTCAT GCTTGATCGG 
ATCTATAGAT TCGACACCGT TATTGCACGG ATCGAAGCTC TTTCTATCGA GCGTTTGCAA 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGAGGACT CTGGCACGGA AACCAAGGCG GGGAATGGGA CCGTTGACAT TTCCGGCTTA 
CCCGACGTAA GATACTCTGC GCTGCTCCAG GAGGACTTTG CCTATTCAGG GTTTGAGTGT 
TGGGTAGAAA ACGAGGATCA GATTCAGGAG CTACACAGCT GCCATACTTC GGAAGGTCCT 
GGAAACCGAC CCAGGAGATG CCGTGGTTGT GCAGCCTTGC GGGTTGCTGA TTCTGACTAT 
GAAGCCATTT GTAAGGTGCC CCGAAAGGTG GCCAGAAGTA TCTCGTGTGC TCCTTCTAGG 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCATTGTCTG AGGTTGGGCC ACCGGATTTA 
CCAAGTACAA AGGTACCCCC AGATGGAGAG AGCATGGAGG AAGGGACACC CGGATCCTCT 
GTGGAATCTT TGGATGCAAG TGTCCAGGCT AGTCCTCCAC AACAAAAGGA TGAGGAAACG 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGTTGTT CAGATGAACA GGCTCCACAG 
CATGTGTGTA ACCACAAGCT AGAGCTAGCT CTTAACATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GTCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAG CCCACCTGGA 
GCCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTCTCCTCAA CAGGTCTTTG 
AAACCCCTTT ACAAGATACC TGTGAATTAT CCCTTGGAGC TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TTTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCTGTGAC TGAAGAGTTA 
CTCAAGCAAC AAAAGCTGGA TTCAGATGAG ACCATTATAA CTCAGCAGTC TGTATCTGAT 
TCCCACTTGG CTGAACTCCA GGAAAAAATC CAGCAAATGG AGGCCACCAA CAAAATTCTT 
CAAGAGAAAC TGAATGAAAT GAGCTGTGAG CTAAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGGTA CAATTCAAAG CCTCAAGGAA ACTCTGAAAA GTAGGGAAAA TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAT GACACAATGG CAAAGCTTCG AGAAATGCTA 
CACCAAAGCC AGCTTGGACA ACTTCATGTA TTTGAGGGTC ACATAGGACA GGAGAGCTCA 
CAGGGTACTT CCCCAGCTCA GCAACAGATG GCTCTGCTTG ATCTTCAGAG TGGTTTGTTC 
TACAGCCAGC TTGAGATTCA GAAACTCCAG AGGTTGGTAC GTCAAAAAGA ACGCCAACTG 
GCTGATGCCA AACGATGTGT CCAATTTGTA GAGGCTGCAG CCCATGAAAG AGAACAGCAG 
AAAGAGGCAT CTTGGAAACA TAATCAGGAA TTACGGAAAG CCTTGCAGCA GCTCCAAGGA 
GAATTAGAGA GTAAGAGCCA ACAGCTTCGT ACCCTGGAGA CTGAAAAATG TAATGAGATC 
AGAGCCCATG AGCAACACAT TCAACACCTA AACCATAGTC TGAGCCACAA AGAGCAGCTG 
CTTCAGGAAT TTCGGGAGCT CCTGCAGTAT CGAGATAAGT CAGACAAAAC CCTTGAAGCA 
AATGAAATGT TGCTTGAGAA ACTTCGGCAG CGAATACAAG ACCGAGATGT TGCTTTGGAG 
CGGGCTATAG ATGAAAAGTT CTCCGCCCTG GAAGAGAAAG AAAAAGAACT GCGTCAATTT 
CATCTTGCTG TGCGAGAGCG TGATCATGAC TTAGAGAGAT TGCGTGCTAT TCTCTCCTCT 
AATGAAGCTA CCATGCAGAG TATGGAGAGT CTCCTGAGGG CCAAAGGCCT AGAAGTAGAG 
CAGTTATCAG CTACCTGTCA AAACCTCCAG TGGCTGAAAG AAGAAAAGGA AACCAAATTT 
AGCCATTGGC AGAAGGAACA AGAAAGTATC ATTCAGCAGT TACAGACATC TCTGCATGAC 
AGGAACAAAG AAGTGGAGGA TCTGAGTGCC ACCTTGTTCT GTAAGCTTGG ACCTGGGCAG 
AGTGAGGTAG CAGAAGAGCT GTGCCAGCGC CTACAGCGAA AGGAAAGGAT GCTACAGGAA 
CTGCTAACCG ATCGAAACAA ACAAGCAGTG GAACACGAAA TAGAGATTCA AGGCCTGCTT 
CAGTCTATGA GCACCAGGGA GCAGGAGAGC CAAGCCGCTG CAGGGAAGAT GGTGCAAGCC 
CTTATGGAAA GAAATTCAGA ACTACAGGCC CTGCGCCAGT ATTTAGGAGG TAAAGACTTC 
ATGGTTTCCC AAGCACTCAT CTCTAACCAA CCCACAGAAG TCACCTCCAA CAGCCCTCGT 
CTTGGAGAGC AGACTGATCA AGGTTCAGTG CATATACCCT CCAAAGATGA TAGCATTTCC 
CTGACTGCCA AAGGGGATAC CAGTATACCC AGGTCCTCAT TAGGAGACCA GGATACAGTT 
GCAGGGCTGG AAAAAGAACT GAGTAATGCC AAAGAGGAGC TTGAGCTCAT GGCTAAGAAA 
GAACGAGAAA GTCGGATGGA ACTTTCTGCA CTACAGTCCA TGGTGGCCGT GCAAGAGGAA 
GAGCTGCAGG TGCAGGCTGC TGACATGGAA TCCCTGACCA GGAATATACA GATTAAAGAA 
GACCTCATAA AGGACCTGCA GATGCAACTG GTTGATCCTG AAGATATACC AGCTATGGAA 
CGCCTGACCC AAGAGGTCTT ACTTCTTCGG GAAAAAGTTG CTTCAGTAGA ATCACAGGGT 
CAGGAAACGT TAGGAAATCG AAGACAACAA TTGCTGCTAA TGCTCGAAGG GCTGGTGGAT 
GAACGGAGTC GGCTCAATGA AGCTTTACAA GCAGAGAGAC AGCTGTACAG CAGCCTGGTG 
AAGTTCCATG CCCATCCAGA GAGCTCTGAG AGAGACCGAA CTCTGCAGGT GGAACTAGAA 
GGGGCCCAGG TGATACGTGG TCGGCTAGAA GAAGTTCTTG GAAGAAGCCT GGAGCGCTTA 
AGCAGGCTGG AGACCCTGGC TGCCATTGGA GGTGCAGCTG CAGGGGAAGA TACAGAAGAT 
GCTAGCACTG AGTTCACGGA CAGCATCGAG GAGGAGGCTG CACACCATAA TCACCAGCAA 
CTCATCAAGG TGGCTTTGGA GAAAAGTCTG GCAACTGTGG AGACTCAGAA CGTATGTCTC 
CCCCCTCCTT CCTCAGCAGG AGGGGACAAT AACAGGGGTC TTCAGGAGGA GATGCTCCAC 
CTGAGGGCTG AGATCCACCA GCACTTAGAG GAGAAGAGGA GAGCTGAGGG GGAACTGAAG 
GAGCTAAAGG CTCAAATTGA GGAAGCAGGA TTCTCCTCTG TGGCCCACAT CAGGAACACC 
ATGCTGAGCC TTTGCCTTGA GAATGCAGAG CTGAAAGAGC AGATGGGAGA AGCAATGTCT 
GATGGATGGG AGATCGAGGA AGACAAGGAG AAGGGCGAGG TGACAGTGGA AACTGTGGCA 
GCCAAAGGAG GTCTGAATGA AAATAGCCTT CAGGCTGAGT TCAGAAAGCT CCAGGGAAAA 
CTGAAGCATG CCCACAACAT TATCAACCTC CTCAAAGAGC AGCTGGTGCT GAGCAGCAAG 
GATGGGAATA GTAAACTTAG TCCAGAGCTC CTAGTGCGCC TGGACCGGGA GATCGACAGA 
ATGAACACAG AGCTGGTCTG TTCTCCGGAG AAGCCCCAGT GCCAGGAAGA AGAGCATATG 
ACCACAAGGC CCTGCCCCAG ACCCCAGAGC CTTGACCTTG GGGCTACCCT CTCAGTGGAT 
GGCCACCAAC TGGATAACCG GCCCCAGGCC CAAGACCCTG GGCCTCAGTC AGAATTTAGC 
CTTCCTGGAT CCACCAAGCA CCTGCGCTCC CAGCTGGCCC AATGCAGACA ACGCTATCAG 
GATCTCCAGG AGAAGCTGCT GATATCGGAA GCCACAGTCT TTGCACAGGC CAACCAACTG 
GAGAAATACC GAGTCATGCT TAGTGAATCC TTGGTGAAGC AGGACAGCAA GCAGGTGCAG 
GTGGACCTCC AGGATCTGGG CTATGAAACC TGTGGCCGAA GTGAGAATGA GGCTGAGCGG 
GAGGAGAGCA CCAGTCCCGA GTGTGAGGAG CCTGACAGCC TCAGGGAAAC AGTCCTGATG 
GTGGGGCACC TGGGCTCACT GCTGGCCAGC CCTCCAGGGA AGAAGCCCTT GGAAAGCCAT 
CGGGGGAAGC AGGAGGAGTT CCAGGCATAT GGAAAGTCAG AAGACATCTC TGTCCTACAT 
AAGGACATCA GAGATCTGAA GGCCCAGCTG CAGAATGCCA ACAAGATCAT TCAGAATCTT 
AAGAGCCGGG TCCGGTCCCT CTCAGTTACA AGTGATTATT CATCTAGTCT GGAGAGACCA 
AGAAAGCTGA AGGCCATTGG CACCCTTGAG GGATCCTCAC CTCACAGTGT CACTGACGAG 
GATGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTGCA CCCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGAGTG TCTCAGCTGG AGGCCCAGCT CCCGAAAACT 
GGAGTGGAAG GGAAGCTAGC AGAGGAGCTG AGATCAGCCT CATGGCCTGG GAAATATGAT 
TCCCTAATTC AGGATCAGGC CCGAGAGCTG TCGTACCTAC GCCAGAAGAT ACGAGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAT GCAAAAGATA CAGTAAAATC TTTTGAGGAC 
CTTCTTAGGA GCAATGACAT TGACTACTAC CTGGGCCAGA GCTTCCGTGA GCAACTTGCC 
CAGGGAAGCC AGCTGACAGA GAGACTCACC AGTAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT TGAACCACTG GCCCTCAGGC TCAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATTGAAGT CCTGCAGGCC AAGCTAGATG CTCGGTCTCT CACACCCTCC 
AGCAGCCATG CATTGTCAGA CTCCCACCGC TCTCCCAGCA GCTCGTCCTT CCTGTCTGAT 
GAGCTGGAAG CCTGCTCTGA CATGGATGTA GCCAGCGAGT ACACACATTA TGAAGAGAAG 
AAGGCTCCAC CTGGTCACTC AGATTCCATC CATCATTTGA GTCATTCTGC TGTACTGTCT 
TCTAAACCAT CAGCAACCAG TGTACCTCGG GGGGTTAAGG CCGAACCCAG CAGCAACCCC 
ATCAGCTTGC CAGCTCCCCA GAACCCCCCC CAAGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTTCCCT CCATATCCAC ACTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCCACTGGC CCTCCCCTCC TTGGCTGCTG TGAGACACCT 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCAGAAACA ATTGGGAGAA 
AGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630747.1
CDS	364..8100
Translation	MKESCRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVPRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNEDSGTETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQELHSCHTSEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVARSISCAPSRWSTSICTEEPALSEVGPPDLPSTKVPPDGESMEEGTPGSSVESLDASVQASPPQQKDEETERSAKELAKCDCCSDEQAPQHVCNHKLELALNMIKGLDYKPIQSPRGSKLPIPVKSSPPGARPGHIMTDGVSSGLLNRSLKPLYKIPVNYPLELSDLQELWDDLCEDYLPLRVQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630690.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630690.3

ORF Insert Sequence:

ATGAAGGAGA GCTGCAGGAT CTGTGCCCGG GAGCTGTGTG GGAACCAGCG GCGCTGGATC 
TTCCACACGG CATCTAAACT CAACCTCCAG GTTCTGCTCT CGCACGTCCT GGGCAAGGAT 
GTGCCCCGCG ATGGCAAAGC CGAGTTTGCT TGCAGCAAAT GTGCTTTCAT GCTTGATCGG 
ATCTATAGAT TCGACACCGT TATTGCACGG ATCGAAGCTC TTTCTATCGA GCGTTTGCAA 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGAGGACT CTGGCACGGA AACCAAGGCG GGGAATGGGA CCGTTGACAT TTCCGGCTTA 
CCCGACGTAA GATACTCTGC GCTGCTCCAG GAGGACTTTG CCTATTCAGG GTTTGAGTGT 
TGGGTAGAAA ACGAGGATCA GATTCAGGAG CTACACAGCT GCCATACTTC GGAAGGTCCT 
GGAAACCGAC CCAGGAGATG CCGTGGTTGT GCAGCCTTGC GGGTTGCTGA TTCTGACTAT 
GAAGCCATTT GTAAGGTGCC CCGAAAGGTG GCCAGAAGTA TCTCGTGTGC TCCTTCTAGG 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCATTGTCTG AGGTTGGGCC ACCGGATTTA 
CCAAGTACAA AGGTACCCCC AGATGGAGAG AGCATGGAGG AAGGGACACC CGGATCCTCT 
GTGGAATCTT TGGATGCAAG TGTCCAGGCT AGTCCTCCAC AACAAAAGGA TGAGGAAACG 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGTTGTT CAGATGAACA GGCTCCACAG 
CATGTGTGTA ACCACAAGCT AGAGCTAGCT CTTAACATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GTCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAG CCCACCTGGA 
GCCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTCTCCTCAA CAGGTCTTTG 
AAACCCCTTT ACAAGATACC TGTGAATTAT CCCTTGGAGC TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TTTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCTGTGAC TGAAGAGTTA 
CTCAAGCAAC AAAAGCTGGA TTCAGATGAG ACCATTATAA CTCAGCAGTC TGTATCTGAT 
TCCCACTTGG CTGAACTCCA GGAAAAAATC CAGCAAATGG AGGCCACCAA CAAAATTCTT 
CAAGAGAAAC TGAATGAAAT GAGCTGTGAG CTAAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGGTA CAATTCAAAG CCTCAAGGAA ACTCTGAAAA GTAGGGAAAA TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAT GACACAATGG CAAAGCTTCG AGAAATGCTA 
CACCAAAGCC AGCTTGGACA ACTTCATGTA TTTGAGGGTC ACATAGGACA GGAGAGCTCA 
CAGGGTACTT CCCCAGCTCA GCAACAGATG GCTCTGCTTG ATCTTCAGAG TGGTTTGTTC 
TACAGCCAGC TTGAGATTCA GAAACTCCAG AGGTTGGTAC GTCAAAAAGA ACGCCAACTG 
GCTGATGCCA AACGATGTGT CCAATTTGTA GAGGCTGCAG CCCATGAAAG AGAACAGCAG 
AAAGAGGCAT CTTGGAAACA TAATCAGGAA TTACGGAAAG CCTTGCAGCA GCTCCAAGGA 
GAATTAGAGA GTAAGAGCCA ACAGCTTCGT ACCCTGGAGA CTGAAAAATG TAATGAGATC 
AGAGCCCATG AGCAACACAT TCAACACCTA AACCATAGTC TGAGCCACAA AGAGCAGCTG 
CTTCAGGAAT TTCGGGAGCT CCTGCAGTAT CGAGATAAGT CAGACAAAAC CCTTGAAGCA 
AATGAAATGT TGCTTGAGAA ACTTCGGCAG CGAATACAAG ACCGAGATGT TGCTTTGGAG 
CGGGCTATAG ATGAAAAGTT CTCCGCCCTG GAAGAGAAAG AAAAAGAACT GCGTCAATTT 
CATCTTGCTG TGCGAGAGCG TGATCATGAC TTAGAGAGAT TGCGTGCTAT TCTCTCCTCT 
AATGAAGCTA CCATGCAGAG TATGGAGAGT CTCCTGAGGG CCAAAGGCCT AGAAGTAGAG 
CAGTTATCAG CTACCTGTCA AAACCTCCAG TGGCTGAAAG AAGAAAAGGA AACCAAATTT 
AGCCATTGGC AGAAGGAACA AGAAAGTATC ATTCAGCAGT TACAGACATC TCTGCATGAC 
AGGAACAAAG AAGTGGAGGA TCTGAGTGCC ACCTTGTTCT GTAAGCTTGG ACCTGGGCAG 
AGTGAGGTAG CAGAAGAGCT GTGCCAGCGC CTACAGCGAA AGGAAAGGAT GCTACAGGAA 
CTGCTAACCG ATCGAAACAA ACAAGCAGTG GAACACGAAA TAGAGATTCA AGGCCTGCTT 
CAGTCTATGA GCACCAGGGA GCAGGAGAGC CAAGCCGCTG CAGGGAAGAT GGTGCAAGCC 
CTTATGGAAA GAAATTCAGA ACTACAGGCC CTGCGCCAGT ATTTAGGAGG TAAAGACTTC 
ATGGTTTCCC AAGCACTCAT CTCTAACCAA CCCACAGAAG TCACCTCCAA CAGCCCTCGT 
CTTGGAGAGC AGACTGATCA AGGTTCAGTG CATATACCCT CCAAAGATGA TAGCATTTCC 
CTGACTGCCA AAGGGGATAC CAGTATACCC AGGTCCTCAT TAGGAGACCA GGATACAGTT 
GCAGGGCTGG AAAAAGAACT GAGTAATGCC AAAGAGGAGC TTGAGCTCAT GGCTAAGAAA 
GAACGAGAAA GTCGGATGGA ACTTTCTGCA CTACAGTCCA TGGTGGCCGT GCAAGAGGAA 
GAGCTGCAGG TGCAGGCTGC TGACATGGAA TCCCTGACCA GGAATATACA GATTAAAGAA 
GACCTCATAA AGGACCTGCA GATGCAACTG GTTGATCCTG AAGATATACC AGCTATGGAA 
CGCCTGACCC AAGAGGTCTT ACTTCTTCGG GAAAAAGTTG CTTCAGTAGA ATCACAGGGT 
CAGGAAACGT TAGGAAATCG AAGACAACAA TTGCTGCTAA TGCTCGAAGG GCTGGTGGAT 
GAACGGAGTC GGCTCAATGA AGCTTTACAA GCAGAGAGAC AGCTGTACAG CAGCCTGGTG 
AAGTTCCATG CCCATCCAGA GAGCTCTGAG AGAGACCGAA CTCTGCAGGT GGAACTAGAA 
GGGGCCCAGG TGATACGTGG TCGGCTAGAA GAAGTTCTTG GAAGAAGCCT GGAGCGCTTA 
AGCAGGCTGG AGACCCTGGC TGCCATTGGA GGTGCAGCTG CAGGGGAAGA TACAGAAGAT 
GCTAGCACTG AGTTCACGGA CAGCATCGAG GAGGAGGCTG CACACCATAA TCACCAGCAA 
CTCATCAAGG TGGCTTTGGA GAAAAGTCTG GCAACTGTGG AGACTCAGAA CGTATGTCTC 
CCCCCTCCTT CCTCAGCAGG AGGGGACAAT AACAGGGGTC TTCAGGAGGA GATGCTCCAC 
CTGAGGGCTG AGATCCACCA GCACTTAGAG GAGAAGAGGA GAGCTGAGGG GGAACTGAAG 
GAGCTAAAGG CTCAAATTGA GGAAGCAGGA TTCTCCTCTG TGGCCCACAT CAGGAACACC 
ATGCTGAGCC TTTGCCTTGA GAATGCAGAG CTGAAAGAGC AGATGGGAGA AGCAATGTCT 
GATGGATGGG AGATCGAGGA AGACAAGGAG AAGGGCGAGG TGACAGTGGA AACTGTGGCA 
GCCAAAGGAG GTCTGAATGA AAATAGCCTT CAGGCTGAGT TCAGAAAGCT CCAGGGAAAA 
CTGAAGCATG CCCACAACAT TATCAACCTC CTCAAAGAGC AGCTGGTGCT GAGCAGCAAG 
GATGGGAATA GTAAACTTAG TCCAGAGCTC CTAGTGCGCC TGGACCGGGA GATCGACAGA 
ATGAACACAG AGCTGGTCTG TTCTCCGGAG AAGCCCCAGT GCCAGGAAGA AGAGCATATG 
ACCACAAGGC CCTGCCCCAG ACCCCAGAGC CTTGACCTTG GGGCTACCCT CTCAGTGGAT 
GGCCACCAAC TGGATAACCG GCCCCAGGCC CAAGACCCTG GGCCTCAGTC AGAATTTAGC 
CTTCCTGGAT CCACCAAGCA CCTGCGCTCC CAGCTGGCCC AATGCAGACA ACGCTATCAG 
GATCTCCAGG AGAAGCTGCT GATATCGGAA GCCACAGTCT TTGCACAGGC CAACCAACTG 
GAGAAATACC GAGTCATGCT TAGTGAATCC TTGGTGAAGC AGGACAGCAA GCAGGTGCAG 
GTGGACCTCC AGGATCTGGG CTATGAAACC TGTGGCCGAA GTGAGAATGA GGCTGAGCGG 
GAGGAGAGCA CCAGTCCCGA GTGTGAGGAG CCTGACAGCC TCAGGGAAAC AGTCCTGATG 
GTGGGGCACC TGGGCTCACT GCTGGCCAGC CCTCCAGGGA AGAAGCCCTT GGAAAGCCAT 
CGGGGGAAGC AGGAGGAGTT CCAGGCATAT GGAAAGTCAG AAGACATCTC TGTCCTACAT 
AAGGACATCA GAGATCTGAA GGCCCAGCTG CAGAATGCCA ACAAGATCAT TCAGAATCTT 
AAGAGCCGGG TCCGGTCCCT CTCAGTTACA AGTGATTATT CATCTAGTCT GGAGAGACCA 
AGAAAGCTGA AGGCCATTGG CACCCTTGAG GGATCCTCAC CTCACAGTGT CACTGACGAG 
GATGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTGCA CCCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGAGTG TCTCAGCTGG AGGCCCAGCT CCCGAAAACT 
GGAGTGGAAG GGAAGCTAGC AGAGGAGCTG AGATCAGCCT CATGGCCTGG GAAATATGAT 
TCCCTAATTC AGGATCAGGC CCGAGAGCTG TCGTACCTAC GCCAGAAGAT ACGAGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAT GCAAAAGATA CAGTAAAATC TTTTGAGGAC 
CTTCTTAGGA GCAATGACAT TGACTACTAC CTGGGCCAGA GCTTCCGTGA GCAACTTGCC 
CAGGGAAGCC AGCTGACAGA GAGACTCACC AGTAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT TGAACCACTG GCCCTCAGGC TCAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATTGAAGT CCTGCAGGCC AAGCTAGATG CTCGGTCTCT CACACCCTCC 
AGCAGCCATG CATTGTCAGA CTCCCACCGC TCTCCCAGCA GCTCGTCCTT CCTGTCTGAT 
GAGCTGGAAG CCTGCTCTGA CATGGATGTA GCCAGCGAGT ACACACATTA TGAAGAGAAG 
AAGGCTCCAC CTGGTCACTC AGATTCCATC CATCATTTGA GTCATTCTGC TGTACTGTCT 
TCTAAACCAT CAGCAACCAG TGTACCTCGG GGGGTTAAGG CCGAACCCAG CAGCAACCCC 
ATCAGCTTGC CAGCTCCCCA GAACCCCCCC CAAGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTTCCCT CCATATCCAC ACTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCCACTGGC CCTCCCCTCC TTGGCTGCTG TGAGACACCT 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCAGAAACA ATTGGGAGAA 
AGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630692.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630749.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X14, mRNA.

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CloneID	OCe19359
Clone ID Related Accession (Same CDS sequence)	XM_005630692.2 , XM_005630692.3
Accession Version	XM_005630692.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAAAG AATTGAAGAA 
CTTCATCAAA GCCTGGCTTC CCAGGAGAAG CTTGTAGAAC AGCTGTCTCA AGAGAAACAA 
CAACTGCTCC ATCTGTTGGA GGAACCAATG AGCATGGAAA TACAGCCTGT GACTGAAGAG 
TTACTCAAGC AACAAAAGCT GGATTCAGAT GAGACCATTA TAACTCAGCA GTCTGTATCT 
GATTCCCACT TGGCTGAACT CCAGGAAAAA ATCCAGCAAA TGGAGGCCAC CAACAAAATT 
CTTCAAGAGA AACTGAATGA AATGAGCTGT GAGCTAAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG GTACAATTCA AAGCCTCAAG GAAACTCTGA AAAGTAGGGA AAATGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AATGACACAA TGGCAAAGCT TCGAGAAATG 
CTACACCAAA GCCAGCTTGG ACAACTTCAT GTATTTGAGG GTCACATAGG ACAGGAGAGC 
TCACAGGGTA CTTCCCCAGC TCAGCAACAG ATGGCTCTGC TTGATCTTCA GAGTGGTTTG 
TTCTACAGCC AGCTTGAGAT TCAGAAACTC CAGAGGTTGG TACGTCAAAA AGAACGCCAA 
CTGGCTGATG CCAAACGATG TGTCCAATTT GTAGAGGCTG CAGCCCATGA AAGAGAACAG 
CAGAAAGAGG CATCTTGGAA ACATAATCAG GAATTACGGA AAGCCTTGCA GCAGCTCCAA 
GGAGAATTAG AGAGTAAGAG CCAACAGCTT CGTACCCTGG AGACTGAAAA ATGTAATGAG 
ATCAGAGCCC ATGAGCAACA CATTCAACAC CTAAACCATA GTCTGAGCCA CAAAGAGCAG 
CTGCTTCAGG AATTTCGGGA GCTCCTGCAG TATCGAGATA AGTCAGACAA AACCCTTGAA 
GCAAATGAAA TGTTGCTTGA GAAACTTCGG CAGCGAATAC AAGACCGAGA TGTTGCTTTG 
GAGCGGGCTA TAGATGAAAA GTTCTCCGCC CTGGAAGAGA AAGAAAAAGA ACTGCGTCAA 
TTTCATCTTG CTGTGCGAGA GCGTGATCAT GACTTAGAGA GATTGCGTGC TATTCTCTCC 
TCTAATGAAG CTACCATGCA GAGTATGGAG AGTCTCCTGA GGGCCAAAGG CCTAGAAGTA 
GAGCAGTTAT CAGCTACCTG TCAAAACCTC CAGTGGCTGA AAGAAGAAAA GGAAACCAAA 
TTTAGCCATT GGCAGAAGGA ACAAGAAAGT ATCATTCAGC AGTTACAGAC ATCTCTGCAT 
GACAGGAACA AAGAAGTGGA GGATCTGAGT GCCACCTTGT TCTGTAAGCT TGGACCTGGG 
CAGAGTGAGG TAGCAGAAGA GCTGTGCCAG CGCCTACAGC GAAAGGAAAG GATGCTACAG 
GAACTGCTAA CCGATCGAAA CAAACAAGCA GTGGAACACG AAATAGAGAT TCAAGGCCTG 
CTTCAGTCTA TGAGCACCAG GGAGCAGGAG AGCCAAGCCG CTGCAGGGAA GATGGTGCAA 
GCCCTTATGG AAAGAAATTC AGAACTACAG GCCCTGCGCC AGTATTTAGG AGGTAAAGAC 
TTCATGGTTT CCCAAGCACT CATCTCTAAC CAACCCACAG AAGTCACCTC CAACAGCCCT 
CGTCTTGGAG AGCAGACTGA TCAAGGTTCA GTGCATATAC CCTCCAAAGA TGATAGCATT 
TCCCTGACTG CCAAAGGGGA TACCAGTATA CCCAGGTCCT CATTAGGAGA CCAGGATACA 
GTTGCAGGGC TGGAAAAAGA ACTGAGTAAT GCCAAAGAGG AGCTTGAGCT CATGGCTAAG 
AAAGAACGAG AAAGTCGGAT GGAACTTTCT GCACTACAGT CCATGGTGGC CGTGCAAGAG 
GAAGAGCTGC AGGTGCAGGC TGCTGACATG GAATCCCTGA CCAGGAATAT ACAGATTAAA 
GAAGACCTCA TAAAGGACCT GCAGATGCAA CTGGTTGATC CTGAAGATAT ACCAGCTATG 
GAACGCCTGA CCCAAGAGGT CTTACTTCTT CGGGAAAAAG TTGCTTCAGT AGAATCACAG 
GGTCAGGAAA CGTTAGGAAA TCGAAGACAA CAATTGCTGC TAATGCTCGA AGGGCTGGTG 
GATGAACGGA GTCGGCTCAA TGAAGCTTTA CAAGCAGAGA GACAGCTGTA CAGCAGCCTG 
GTGAAGTTCC ATGCCCATCC AGAGAGCTCT GAGAGAGACC GAACTCTGCA GGTGGAACTA 
GAAGGGGCCC AGGTGATACG TGGTCGGCTA GAAGAAGTTC TTGGAAGAAG CCTGGAGCGC 
TTAAGCAGGC TGGAGACCCT GGCTGCCATT GGAGGTGCAG CTGCAGGGGA AGATACAGAA 
GATGCTAGCA CTGAGTTCAC GGACAGCATC GAGGAGGAGG CTGCACACCA TAATCACCAG 
CAACTCATCA AGGTGGCTTT GGAGAAAAGT CTGGCAACTG TGGAGACTCA GAACGTATGT 
CTCCCCCCTC CTTCCTCAGC AGGAGGGGAC AATAACAGGG GTCTTCAGGA GGAGATGCTC 
CACCTGAGGG CTGAGATCCA CCAGCACTTA GAGGAGAAGA GGAGAGCTGA GGGGGAACTG 
AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA GGATTCTCCT CTGTGGCCCA CATCAGGAAC 
ACCATGCTGA GCCTTTGCCT TGAGAATGCA GAGCTGAAAG AGCAGATGGG AGAAGCAATG 
TCTGATGGAT GGGAGATCGA GGAAGACAAG GAGAAGGGCG AGGTGACAGT GGAAACTGTG 
GCAGCCAAAG GAGGTCTGAA TGAAAATAGC CTTCAGGCTG AGTTCAGAAA GCTCCAGGGA 
AAACTGAAGC ATGCCCACAA CATTATCAAC CTCCTCAAAG AGCAGCTGGT GCTGAGCAGC 
AAGGATGGGA ATAGTAAACT TAGTCCAGAG CTCCTAGTGC GCCTGGACCG GGAGATCGAC 
AGAATGAACA CAGAGCTGGT CTGTTCTCCG GAGAAGCCCC AGTGCCAGGA AGAAGAGCAT 
ATGACCACAA GGCCCTGCCC CAGACCCCAG AGCCTTGACC TTGGGGCTAC CCTCTCAGTG 
GATGGCCACC AACTGGATAA CCGGCCCCAG GCCCAAGACC CTGGGCCTCA GTCAGAATTT 
AGCCTTCCTG GATCCACCAA GCACCTGCGC TCCCAGCTGG CCCAATGCAG ACAACGCTAT 
CAGGATCTCC AGGAGAAGCT GCTGATATCG GAAGCCACAG TCTTTGCACA GGCCAACCAA 
CTGGAGAAAT ACCGAGTCAT GCTTAGTGAA TCCTTGGTGA AGCAGGACAG CAAGCAGGTG 
CAGGTGGACC TCCAGGATCT GGGCTATGAA ACCTGTGGCC GAAGTGAGAA TGAGGCTGAG 
CGGGAGGAGA GCACCAGTCC CGAGTGTGAG GAGCCTGACA GCCTCAGGGA AACAGTCCTG 
ATGGTGGGGC ACCTGGGCTC ACTGCTGGCC AGCCCTCCAG GGAAGAAGCC CTTGGAAAGC 
CATCGGGGGA AGCAGGAGGA GTTCCAGGCA TATGGAAAGT CAGAAGACAT CTCTGTCCTA 
CATAAGGACA TCAGAGATCT GAAGGCCCAG CTGCAGAATG CCAACAAGAT CATTCAGAAT 
CTTAAGAGCC GGGTCCGGTC CCTCTCAGTT ACAAGTGATT ATTCATCTAG TCTGGAGAGA 
CCAAGAAAGC TGAAGGCCAT TGGCACCCTT GAGGGATCCT CACCTCACAG TGTCACTGAC 
GAGGATGAGG GGTGGCTGTC TGATGGCACT GGGGCTTTCT GCACCCCAGG GCTTCAGGCT 
AAAAAGGATC TGGAGAGTCT GATCCAGAGA GTGTCTCAGC TGGAGGCCCA GCTCCCGAAA 
ACTGGAGTGG AAGGGAAGCT AGCAGAGGAG CTGAGATCAG CCTCATGGCC TGGGAAATAT 
GATTCCCTAA TTCAGGATCA GGCCCGAGAG CTGTCGTACC TACGCCAGAA GATACGAGAA 
GGGAGAGGTA TCTGTTATCT TCTCACCCAG CATGCAAAAG ATACAGTAAA ATCTTTTGAG 
GACCTTCTTA GGAGCAATGA CATTGACTAC TACCTGGGCC AGAGCTTCCG TGAGCAACTT 
GCCCAGGGAA GCCAGCTGAC AGAGAGACTC ACCAGTAAAC TCAGCACCAA GGATCATAAA 
AGTGAGAAAG ATCAAGCTGG ACTTGAACCA CTGGCCCTCA GGCTCAGCAG GGAGCTGCAG 
GAGAAGGAGA AAGTGATTGA AGTCCTGCAG GCCAAGCTAG ATGCTCGGTC TCTCACACCC 
TCCAGCAGCC ATGCATTGTC AGACTCCCAC CGCTCTCCCA GCAGCTCGTC CTTCCTGTCT 
GATGAGCTGG AAGCCTGCTC TGACATGGAT GTAGCCAGCG AGTACACACA TTATGAAGAG 
AAGAAGGCTC CACCTGGTCA CTCAGATTCC ATCCATCATT TGAGTCATTC TGCTGTACTG 
TCTTCTAAAC CATCAGCAAC CAGTGTACCT CGGGGGGTTA AGGCCGAACC CAGCAGCAAC 
CCCATCAGCT TGCCAGCTCC CCAGAACCCC CCCCAAGAGG CCAGCCAGGC CCATCCAGGC 
CTTCATTTTC CCTCCATATC CACACTGGTT AGCCTTCCCC AGGCACCACT GCCCTCAGCT 
CCACCCAGCT TCCTGCCTTT CAGCTCCACT GGCCCTCCCC TCCTTGGCTG CTGTGAGACA 
CCTATGGTCT CCTTGGCAGA GGCTCAGCAG GAGCTGCAGA TGCTGCAGAA ACAATTGGGA 
GAAAGTGTCA GCACCGTCCA TCCTGCTTCC CCAGCTGCAT TGCCAAGCAA CCATTTAGAA 
GCCAACTCTT CCCAATATCT CAACCCTGCC CAGCCCCACT CTCCTCCAAC TGGCAGCACA 
GAATTGGGCA GAATCCTGGA GCCTGGCTAC CTGGGTAGCG GCAGCCAGTG GGATGTGATG 
AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC TTATCCTCAG GATCCTCTGT GTGTCAGCTT 
AACTCCAAAC CCACAGGGGC TGACCTGCTA GAAGAACATC TTGGTGAAAT CCGGAACCTG 
CGCCAGCGCC TAGAGGAGTC CATCTGCATC AATGACCGCC TGCGGGAACA GCTGGAACAC 
CGGCTCAGCT GCACAGCACG TGGCAATGGA TCCACCTCTA ATTTCTACAG TCCTGGCCTG 
GAACCCACAC CTCAGCTCTA CAATGAGAAC AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT 
CAGGCTCAGC TGAGTCATGT TTCTAGAGAG CGCTCCCAGG AGACAGAATG CCTGAGGGAG 
GCTCTACTGT CCTCTCAATC CCGCCTTCAA GAGCTGGAAA TGGAGCTAGA GCACCAAAAG 
GAGGAACGGC AGCAGCTTTT GGAAGACTTG AAGGAGAAGC AGCAGGAGAT CTTGCACTTC 
CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA GACTCTAGGC TGCAGCGTAA GCTGGCCCTA 
CTGCAGCAAC AGTGTGAAGA GAAACAGCAG CTCTTCCAGT CCCTCCAGTC AGAGCTACAG 
ATCTATGAGG CGCTTTATGG CAGTTCCAAG AAGGGTCTGA AAGCTTCCAC TTGGGATGCC 
TATCACCAGG TCCCTTTGAG CAGTGACTTG AGCCACCTGG TGGCAGAGAT CCGAGCTTTG 
CGAGGGCAGC TGGAGCAAAG CATACAGGTG AATAACTGTC TGCGACTGCA GCTGGAGCAG 
CAGCTGGATG GCGGAAGGAG CAAAACCAGC CTCAGTCCCT CTTCCACCAG CCAGGAGGAC 
CCTGGAGACA AGCAAGACTC AATTGCTTCC CCTCCAGTCC GAGATGTTGG CATGAATTCT 
CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT GCTGCTCCAG GCTCAGAGAC AGCTATTTTC 
AATAGGATAA ATGAGCTGAA TTTGGATGCT TTTCCAGTAA TGAAGAACTC TCCCAAGCTG 
GAGGGCGAAG CTATCGATGG CTCCTTTGCC AATAAGCACG GCCGCCATGT GATTGGCCAC 
ATTGATGACT ATAGTGCCCT AAGACAGCAG ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG 
ATAACAGCAC TTGTGAGATC AGCCTACACC CTCCCTGGCC TTGAAACTCA GGCGACAGAG 
GTGCCAGGTG GCAAAGGCAT CCATGAACTT GGGAGCAGCA CCCAAGCCCT GCAGCACGTG 
CTGGAGGAGT CGGCCTCCCT CCTCAATATG TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC 
CCTGCCCTGT CTGGCAACAT GGGACAGGCC ATGAAAAAGG AACTTCTGGA ACTGAGAACC 
AAACTGACCA AACAGGAGAC CCTCCTTCAG TGCACAGCTG AGCGTCTAAA GACCACCCAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCATT TGACCAGGAC ACATGATGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGGCC AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC 
ACTTACAAGA TGGCCTCTGT AAAGCCTTAT TCCTGCCCCA GCAAAGGAGC CACGCAAGAA 
GAGGAGCCCC CAGAGGTCCT TCAAACAGCA GGAAGGATGG GCCTGCCCCC CTTTTGTGCA 
GGTCCCAATC TGCTGAGGAG GCTTGGACCC CACTCCTCCG CACTGGCTGG AGGGTCCAGG 
AGGAGTCAGG CATGGCCCAG TGGACGAGGG GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630749.1
CDS	16..7275
Translation	MFLCQGFRKYLPEHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630692.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630692.2

ORF Insert Sequence:

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAAAG AATTGAAGAA 
CTTCATCAAA GCCTGGCTTC CCAGGAGAAG CTTGTAGAAC AGCTGTCTCA AGAGAAACAA 
CAACTGCTCC ATCTGTTGGA GGAACCAATG AGCATGGAAA TACAGCCTGT GACTGAAGAG 
TTACTCAAGC AACAAAAGCT GGATTCAGAT GAGACCATTA TAACTCAGCA GTCTGTATCT 
GATTCCCACT TGGCTGAACT CCAGGAAAAA ATCCAGCAAA TGGAGGCCAC CAACAAAATT 
CTTCAAGAGA AACTGAATGA AATGAGCTGT GAGCTAAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG GTACAATTCA AAGCCTCAAG GAAACTCTGA AAAGTAGGGA AAATGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AATGACACAA TGGCAAAGCT TCGAGAAATG 
CTACACCAAA GCCAGCTTGG ACAACTTCAT GTATTTGAGG GTCACATAGG ACAGGAGAGC 
TCACAGGGTA CTTCCCCAGC TCAGCAACAG ATGGCTCTGC TTGATCTTCA GAGTGGTTTG 
TTCTACAGCC AGCTTGAGAT TCAGAAACTC CAGAGGTTGG TACGTCAAAA AGAACGCCAA 
CTGGCTGATG CCAAACGATG TGTCCAATTT GTAGAGGCTG CAGCCCATGA AAGAGAACAG 
CAGAAAGAGG CATCTTGGAA ACATAATCAG GAATTACGGA AAGCCTTGCA GCAGCTCCAA 
GGAGAATTAG AGAGTAAGAG CCAACAGCTT CGTACCCTGG AGACTGAAAA ATGTAATGAG 
ATCAGAGCCC ATGAGCAACA CATTCAACAC CTAAACCATA GTCTGAGCCA CAAAGAGCAG 
CTGCTTCAGG AATTTCGGGA GCTCCTGCAG TATCGAGATA AGTCAGACAA AACCCTTGAA 
GCAAATGAAA TGTTGCTTGA GAAACTTCGG CAGCGAATAC AAGACCGAGA TGTTGCTTTG 
GAGCGGGCTA TAGATGAAAA GTTCTCCGCC CTGGAAGAGA AAGAAAAAGA ACTGCGTCAA 
TTTCATCTTG CTGTGCGAGA GCGTGATCAT GACTTAGAGA GATTGCGTGC TATTCTCTCC 
TCTAATGAAG CTACCATGCA GAGTATGGAG AGTCTCCTGA GGGCCAAAGG CCTAGAAGTA 
GAGCAGTTAT CAGCTACCTG TCAAAACCTC CAGTGGCTGA AAGAAGAAAA GGAAACCAAA 
TTTAGCCATT GGCAGAAGGA ACAAGAAAGT ATCATTCAGC AGTTACAGAC ATCTCTGCAT 
GACAGGAACA AAGAAGTGGA GGATCTGAGT GCCACCTTGT TCTGTAAGCT TGGACCTGGG 
CAGAGTGAGG TAGCAGAAGA GCTGTGCCAG CGCCTACAGC GAAAGGAAAG GATGCTACAG 
GAACTGCTAA CCGATCGAAA CAAACAAGCA GTGGAACACG AAATAGAGAT TCAAGGCCTG 
CTTCAGTCTA TGAGCACCAG GGAGCAGGAG AGCCAAGCCG CTGCAGGGAA GATGGTGCAA 
GCCCTTATGG AAAGAAATTC AGAACTACAG GCCCTGCGCC AGTATTTAGG AGGTAAAGAC 
TTCATGGTTT CCCAAGCACT CATCTCTAAC CAACCCACAG AAGTCACCTC CAACAGCCCT 
CGTCTTGGAG AGCAGACTGA TCAAGGTTCA GTGCATATAC CCTCCAAAGA TGATAGCATT 
TCCCTGACTG CCAAAGGGGA TACCAGTATA CCCAGGTCCT CATTAGGAGA CCAGGATACA 
GTTGCAGGGC TGGAAAAAGA ACTGAGTAAT GCCAAAGAGG AGCTTGAGCT CATGGCTAAG 
AAAGAACGAG AAAGTCGGAT GGAACTTTCT GCACTACAGT CCATGGTGGC CGTGCAAGAG 
GAAGAGCTGC AGGTGCAGGC TGCTGACATG GAATCCCTGA CCAGGAATAT ACAGATTAAA 
GAAGACCTCA TAAAGGACCT GCAGATGCAA CTGGTTGATC CTGAAGATAT ACCAGCTATG 
GAACGCCTGA CCCAAGAGGT CTTACTTCTT CGGGAAAAAG TTGCTTCAGT AGAATCACAG 
GGTCAGGAAA CGTTAGGAAA TCGAAGACAA CAATTGCTGC TAATGCTCGA AGGGCTGGTG 
GATGAACGGA GTCGGCTCAA TGAAGCTTTA CAAGCAGAGA GACAGCTGTA CAGCAGCCTG 
GTGAAGTTCC ATGCCCATCC AGAGAGCTCT GAGAGAGACC GAACTCTGCA GGTGGAACTA 
GAAGGGGCCC AGGTGATACG TGGTCGGCTA GAAGAAGTTC TTGGAAGAAG CCTGGAGCGC 
TTAAGCAGGC TGGAGACCCT GGCTGCCATT GGAGGTGCAG CTGCAGGGGA AGATACAGAA 
GATGCTAGCA CTGAGTTCAC GGACAGCATC GAGGAGGAGG CTGCACACCA TAATCACCAG 
CAACTCATCA AGGTGGCTTT GGAGAAAAGT CTGGCAACTG TGGAGACTCA GAACGTATGT 
CTCCCCCCTC CTTCCTCAGC AGGAGGGGAC AATAACAGGG GTCTTCAGGA GGAGATGCTC 
CACCTGAGGG CTGAGATCCA CCAGCACTTA GAGGAGAAGA GGAGAGCTGA GGGGGAACTG 
AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA GGATTCTCCT CTGTGGCCCA CATCAGGAAC 
ACCATGCTGA GCCTTTGCCT TGAGAATGCA GAGCTGAAAG AGCAGATGGG AGAAGCAATG 
TCTGATGGAT GGGAGATCGA GGAAGACAAG GAGAAGGGCG AGGTGACAGT GGAAACTGTG 
GCAGCCAAAG GAGGTCTGAA TGAAAATAGC CTTCAGGCTG AGTTCAGAAA GCTCCAGGGA 
AAACTGAAGC ATGCCCACAA CATTATCAAC CTCCTCAAAG AGCAGCTGGT GCTGAGCAGC 
AAGGATGGGA ATAGTAAACT TAGTCCAGAG CTCCTAGTGC GCCTGGACCG GGAGATCGAC 
AGAATGAACA CAGAGCTGGT CTGTTCTCCG GAGAAGCCCC AGTGCCAGGA AGAAGAGCAT 
ATGACCACAA GGCCCTGCCC CAGACCCCAG AGCCTTGACC TTGGGGCTAC CCTCTCAGTG 
GATGGCCACC AACTGGATAA CCGGCCCCAG GCCCAAGACC CTGGGCCTCA GTCAGAATTT 
AGCCTTCCTG GATCCACCAA GCACCTGCGC TCCCAGCTGG CCCAATGCAG ACAACGCTAT 
CAGGATCTCC AGGAGAAGCT GCTGATATCG GAAGCCACAG TCTTTGCACA GGCCAACCAA 
CTGGAGAAAT ACCGAGTCAT GCTTAGTGAA TCCTTGGTGA AGCAGGACAG CAAGCAGGTG 
CAGGTGGACC TCCAGGATCT GGGCTATGAA ACCTGTGGCC GAAGTGAGAA TGAGGCTGAG 
CGGGAGGAGA GCACCAGTCC CGAGTGTGAG GAGCCTGACA GCCTCAGGGA AACAGTCCTG 
ATGGTGGGGC ACCTGGGCTC ACTGCTGGCC AGCCCTCCAG GGAAGAAGCC CTTGGAAAGC 
CATCGGGGGA AGCAGGAGGA GTTCCAGGCA TATGGAAAGT CAGAAGACAT CTCTGTCCTA 
CATAAGGACA TCAGAGATCT GAAGGCCCAG CTGCAGAATG CCAACAAGAT CATTCAGAAT 
CTTAAGAGCC GGGTCCGGTC CCTCTCAGTT ACAAGTGATT ATTCATCTAG TCTGGAGAGA 
CCAAGAAAGC TGAAGGCCAT TGGCACCCTT GAGGGATCCT CACCTCACAG TGTCACTGAC 
GAGGATGAGG GGTGGCTGTC TGATGGCACT GGGGCTTTCT GCACCCCAGG GCTTCAGGCT 
AAAAAGGATC TGGAGAGTCT GATCCAGAGA GTGTCTCAGC TGGAGGCCCA GCTCCCGAAA 
ACTGGAGTGG AAGGGAAGCT AGCAGAGGAG CTGAGATCAG CCTCATGGCC TGGGAAATAT 
GATTCCCTAA TTCAGGATCA GGCCCGAGAG CTGTCGTACC TACGCCAGAA GATACGAGAA 
GGGAGAGGTA TCTGTTATCT TCTCACCCAG CATGCAAAAG ATACAGTAAA ATCTTTTGAG 
GACCTTCTTA GGAGCAATGA CATTGACTAC TACCTGGGCC AGAGCTTCCG TGAGCAACTT 
GCCCAGGGAA GCCAGCTGAC AGAGAGACTC ACCAGTAAAC TCAGCACCAA GGATCATAAA 
AGTGAGAAAG ATCAAGCTGG ACTTGAACCA CTGGCCCTCA GGCTCAGCAG GGAGCTGCAG 
GAGAAGGAGA AAGTGATTGA AGTCCTGCAG GCCAAGCTAG ATGCTCGGTC TCTCACACCC 
TCCAGCAGCC ATGCATTGTC AGACTCCCAC CGCTCTCCCA GCAGCTCGTC CTTCCTGTCT 
GATGAGCTGG AAGCCTGCTC TGACATGGAT GTAGCCAGCG AGTACACACA TTATGAAGAG 
AAGAAGGCTC CACCTGGTCA CTCAGATTCC ATCCATCATT TGAGTCATTC TGCTGTACTG 
TCTTCTAAAC CATCAGCAAC CAGTGTACCT CGGGGGGTTA AGGCCGAACC CAGCAGCAAC 
CCCATCAGCT TGCCAGCTCC CCAGAACCCC CCCCAAGAGG CCAGCCAGGC CCATCCAGGC 
CTTCATTTTC CCTCCATATC CACACTGGTT AGCCTTCCCC AGGCACCACT GCCCTCAGCT 
CCACCCAGCT TCCTGCCTTT CAGCTCCACT GGCCCTCCCC TCCTTGGCTG CTGTGAGACA 
CCTATGGTCT CCTTGGCAGA GGCTCAGCAG GAGCTGCAGA TGCTGCAGAA ACAATTGGGA 
GAAAGTGTCA GCACCGTCCA TCCTGCTTCC CCAGCTGCAT TGCCAAGCAA CCATTTAGAA 
GCCAACTCTT CCCAATATCT CAACCCTGCC CAGCCCCACT CTCCTCCAAC TGGCAGCACA 
GAATTGGGCA GAATCCTGGA GCCTGGCTAC CTGGGTAGCG GCAGCCAGTG GGATGTGATG 
AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC TTATCCTCAG GATCCTCTGT GTGTCAGCTT 
AACTCCAAAC CCACAGGGGC TGACCTGCTA GAAGAACATC TTGGTGAAAT CCGGAACCTG 
CGCCAGCGCC TAGAGGAGTC CATCTGCATC AATGACCGCC TGCGGGAACA GCTGGAACAC 
CGGCTCAGCT GCACAGCACG TGGCAATGGA TCCACCTCTA ATTTCTACAG TCCTGGCCTG 
GAACCCACAC CTCAGCTCTA CAATGAGAAC AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT 
CAGGCTCAGC TGAGTCATGT TTCTAGAGAG CGCTCCCAGG AGACAGAATG CCTGAGGGAG 
GCTCTACTGT CCTCTCAATC CCGCCTTCAA GAGCTGGAAA TGGAGCTAGA GCACCAAAAG 
GAGGAACGGC AGCAGCTTTT GGAAGACTTG AAGGAGAAGC AGCAGGAGAT CTTGCACTTC 
CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA GACTCTAGGC TGCAGCGTAA GCTGGCCCTA 
CTGCAGCAAC AGTGTGAAGA GAAACAGCAG CTCTTCCAGT CCCTCCAGTC AGAGCTACAG 
ATCTATGAGG CGCTTTATGG CAGTTCCAAG AAGGGTCTGA AAGCTTCCAC TTGGGATGCC 
TATCACCAGG TCCCTTTGAG CAGTGACTTG AGCCACCTGG TGGCAGAGAT CCGAGCTTTG 
CGAGGGCAGC TGGAGCAAAG CATACAGGTG AATAACTGTC TGCGACTGCA GCTGGAGCAG 
CAGCTGGATG GCGGAAGGAG CAAAACCAGC CTCAGTCCCT CTTCCACCAG CCAGGAGGAC 
CCTGGAGACA AGCAAGACTC AATTGCTTCC CCTCCAGTCC GAGATGTTGG CATGAATTCT 
CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT GCTGCTCCAG GCTCAGAGAC AGCTATTTTC 
AATAGGATAA ATGAGCTGAA TTTGGATGCT TTTCCAGTAA TGAAGAACTC TCCCAAGCTG 
GAGGGCGAAG CTATCGATGG CTCCTTTGCC AATAAGCACG GCCGCCATGT GATTGGCCAC 
ATTGATGACT ATAGTGCCCT AAGACAGCAG ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG 
ATAACAGCAC TTGTGAGATC AGCCTACACC CTCCCTGGCC TTGAAACTCA GGCGACAGAG 
GTGCCAGGTG GCAAAGGCAT CCATGAACTT GGGAGCAGCA CCCAAGCCCT GCAGCACGTG 
CTGGAGGAGT CGGCCTCCCT CCTCAATATG TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC 
CCTGCCCTGT CTGGCAACAT GGGACAGGCC ATGAAAAAGG AACTTCTGGA ACTGAGAACC 
AAACTGACCA AACAGGAGAC CCTCCTTCAG TGCACAGCTG AGCGTCTAAA GACCACCCAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCATT TGACCAGGAC ACATGATGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGGCC AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC 
ACTTACAAGA TGGCCTCTGT AAAGCCTTAT TCCTGCCCCA GCAAAGGAGC CACGCAAGAA 
GAGGAGCCCC CAGAGGTCCT TCAAACAGCA GGAAGGATGG GCCTGCCCCC CTTTTGTGCA 
GGTCCCAATC TGCTGAGGAG GCTTGGACCC CACTCCTCCG CACTGGCTGG AGGGTCCAGG 
AGGAGTCAGG CATGGCCCAG TGGACGAGGG GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630692.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630749.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X14, mRNA.

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CloneID	OCe19359
Clone ID Related Accession (Same CDS sequence)	XM_005630692.2 , XM_005630692.3
Accession Version	XM_005630692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAAAG AATTGAAGAA 
CTTCATCAAA GCCTGGCTTC CCAGGAGAAG CTTGTAGAAC AGCTGTCTCA AGAGAAACAA 
CAACTGCTCC ATCTGTTGGA GGAACCAATG AGCATGGAAA TACAGCCTGT GACTGAAGAG 
TTACTCAAGC AACAAAAGCT GGATTCAGAT GAGACCATTA TAACTCAGCA GTCTGTATCT 
GATTCCCACT TGGCTGAACT CCAGGAAAAA ATCCAGCAAA TGGAGGCCAC CAACAAAATT 
CTTCAAGAGA AACTGAATGA AATGAGCTGT GAGCTAAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG GTACAATTCA AAGCCTCAAG GAAACTCTGA AAAGTAGGGA AAATGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AATGACACAA TGGCAAAGCT TCGAGAAATG 
CTACACCAAA GCCAGCTTGG ACAACTTCAT GTATTTGAGG GTCACATAGG ACAGGAGAGC 
TCACAGGGTA CTTCCCCAGC TCAGCAACAG ATGGCTCTGC TTGATCTTCA GAGTGGTTTG 
TTCTACAGCC AGCTTGAGAT TCAGAAACTC CAGAGGTTGG TACGTCAAAA AGAACGCCAA 
CTGGCTGATG CCAAACGATG TGTCCAATTT GTAGAGGCTG CAGCCCATGA AAGAGAACAG 
CAGAAAGAGG CATCTTGGAA ACATAATCAG GAATTACGGA AAGCCTTGCA GCAGCTCCAA 
GGAGAATTAG AGAGTAAGAG CCAACAGCTT CGTACCCTGG AGACTGAAAA ATGTAATGAG 
ATCAGAGCCC ATGAGCAACA CATTCAACAC CTAAACCATA GTCTGAGCCA CAAAGAGCAG 
CTGCTTCAGG AATTTCGGGA GCTCCTGCAG TATCGAGATA AGTCAGACAA AACCCTTGAA 
GCAAATGAAA TGTTGCTTGA GAAACTTCGG CAGCGAATAC AAGACCGAGA TGTTGCTTTG 
GAGCGGGCTA TAGATGAAAA GTTCTCCGCC CTGGAAGAGA AAGAAAAAGA ACTGCGTCAA 
TTTCATCTTG CTGTGCGAGA GCGTGATCAT GACTTAGAGA GATTGCGTGC TATTCTCTCC 
TCTAATGAAG CTACCATGCA GAGTATGGAG AGTCTCCTGA GGGCCAAAGG CCTAGAAGTA 
GAGCAGTTAT CAGCTACCTG TCAAAACCTC CAGTGGCTGA AAGAAGAAAA GGAAACCAAA 
TTTAGCCATT GGCAGAAGGA ACAAGAAAGT ATCATTCAGC AGTTACAGAC ATCTCTGCAT 
GACAGGAACA AAGAAGTGGA GGATCTGAGT GCCACCTTGT TCTGTAAGCT TGGACCTGGG 
CAGAGTGAGG TAGCAGAAGA GCTGTGCCAG CGCCTACAGC GAAAGGAAAG GATGCTACAG 
GAACTGCTAA CCGATCGAAA CAAACAAGCA GTGGAACACG AAATAGAGAT TCAAGGCCTG 
CTTCAGTCTA TGAGCACCAG GGAGCAGGAG AGCCAAGCCG CTGCAGGGAA GATGGTGCAA 
GCCCTTATGG AAAGAAATTC AGAACTACAG GCCCTGCGCC AGTATTTAGG AGGTAAAGAC 
TTCATGGTTT CCCAAGCACT CATCTCTAAC CAACCCACAG AAGTCACCTC CAACAGCCCT 
CGTCTTGGAG AGCAGACTGA TCAAGGTTCA GTGCATATAC CCTCCAAAGA TGATAGCATT 
TCCCTGACTG CCAAAGGGGA TACCAGTATA CCCAGGTCCT CATTAGGAGA CCAGGATACA 
GTTGCAGGGC TGGAAAAAGA ACTGAGTAAT GCCAAAGAGG AGCTTGAGCT CATGGCTAAG 
AAAGAACGAG AAAGTCGGAT GGAACTTTCT GCACTACAGT CCATGGTGGC CGTGCAAGAG 
GAAGAGCTGC AGGTGCAGGC TGCTGACATG GAATCCCTGA CCAGGAATAT ACAGATTAAA 
GAAGACCTCA TAAAGGACCT GCAGATGCAA CTGGTTGATC CTGAAGATAT ACCAGCTATG 
GAACGCCTGA CCCAAGAGGT CTTACTTCTT CGGGAAAAAG TTGCTTCAGT AGAATCACAG 
GGTCAGGAAA CGTTAGGAAA TCGAAGACAA CAATTGCTGC TAATGCTCGA AGGGCTGGTG 
GATGAACGGA GTCGGCTCAA TGAAGCTTTA CAAGCAGAGA GACAGCTGTA CAGCAGCCTG 
GTGAAGTTCC ATGCCCATCC AGAGAGCTCT GAGAGAGACC GAACTCTGCA GGTGGAACTA 
GAAGGGGCCC AGGTGATACG TGGTCGGCTA GAAGAAGTTC TTGGAAGAAG CCTGGAGCGC 
TTAAGCAGGC TGGAGACCCT GGCTGCCATT GGAGGTGCAG CTGCAGGGGA AGATACAGAA 
GATGCTAGCA CTGAGTTCAC GGACAGCATC GAGGAGGAGG CTGCACACCA TAATCACCAG 
CAACTCATCA AGGTGGCTTT GGAGAAAAGT CTGGCAACTG TGGAGACTCA GAACGTATGT 
CTCCCCCCTC CTTCCTCAGC AGGAGGGGAC AATAACAGGG GTCTTCAGGA GGAGATGCTC 
CACCTGAGGG CTGAGATCCA CCAGCACTTA GAGGAGAAGA GGAGAGCTGA GGGGGAACTG 
AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA GGATTCTCCT CTGTGGCCCA CATCAGGAAC 
ACCATGCTGA GCCTTTGCCT TGAGAATGCA GAGCTGAAAG AGCAGATGGG AGAAGCAATG 
TCTGATGGAT GGGAGATCGA GGAAGACAAG GAGAAGGGCG AGGTGACAGT GGAAACTGTG 
GCAGCCAAAG GAGGTCTGAA TGAAAATAGC CTTCAGGCTG AGTTCAGAAA GCTCCAGGGA 
AAACTGAAGC ATGCCCACAA CATTATCAAC CTCCTCAAAG AGCAGCTGGT GCTGAGCAGC 
AAGGATGGGA ATAGTAAACT TAGTCCAGAG CTCCTAGTGC GCCTGGACCG GGAGATCGAC 
AGAATGAACA CAGAGCTGGT CTGTTCTCCG GAGAAGCCCC AGTGCCAGGA AGAAGAGCAT 
ATGACCACAA GGCCCTGCCC CAGACCCCAG AGCCTTGACC TTGGGGCTAC CCTCTCAGTG 
GATGGCCACC AACTGGATAA CCGGCCCCAG GCCCAAGACC CTGGGCCTCA GTCAGAATTT 
AGCCTTCCTG GATCCACCAA GCACCTGCGC TCCCAGCTGG CCCAATGCAG ACAACGCTAT 
CAGGATCTCC AGGAGAAGCT GCTGATATCG GAAGCCACAG TCTTTGCACA GGCCAACCAA 
CTGGAGAAAT ACCGAGTCAT GCTTAGTGAA TCCTTGGTGA AGCAGGACAG CAAGCAGGTG 
CAGGTGGACC TCCAGGATCT GGGCTATGAA ACCTGTGGCC GAAGTGAGAA TGAGGCTGAG 
CGGGAGGAGA GCACCAGTCC CGAGTGTGAG GAGCCTGACA GCCTCAGGGA AACAGTCCTG 
ATGGTGGGGC ACCTGGGCTC ACTGCTGGCC AGCCCTCCAG GGAAGAAGCC CTTGGAAAGC 
CATCGGGGGA AGCAGGAGGA GTTCCAGGCA TATGGAAAGT CAGAAGACAT CTCTGTCCTA 
CATAAGGACA TCAGAGATCT GAAGGCCCAG CTGCAGAATG CCAACAAGAT CATTCAGAAT 
CTTAAGAGCC GGGTCCGGTC CCTCTCAGTT ACAAGTGATT ATTCATCTAG TCTGGAGAGA 
CCAAGAAAGC TGAAGGCCAT TGGCACCCTT GAGGGATCCT CACCTCACAG TGTCACTGAC 
GAGGATGAGG GGTGGCTGTC TGATGGCACT GGGGCTTTCT GCACCCCAGG GCTTCAGGCT 
AAAAAGGATC TGGAGAGTCT GATCCAGAGA GTGTCTCAGC TGGAGGCCCA GCTCCCGAAA 
ACTGGAGTGG AAGGGAAGCT AGCAGAGGAG CTGAGATCAG CCTCATGGCC TGGGAAATAT 
GATTCCCTAA TTCAGGATCA GGCCCGAGAG CTGTCGTACC TACGCCAGAA GATACGAGAA 
GGGAGAGGTA TCTGTTATCT TCTCACCCAG CATGCAAAAG ATACAGTAAA ATCTTTTGAG 
GACCTTCTTA GGAGCAATGA CATTGACTAC TACCTGGGCC AGAGCTTCCG TGAGCAACTT 
GCCCAGGGAA GCCAGCTGAC AGAGAGACTC ACCAGTAAAC TCAGCACCAA GGATCATAAA 
AGTGAGAAAG ATCAAGCTGG ACTTGAACCA CTGGCCCTCA GGCTCAGCAG GGAGCTGCAG 
GAGAAGGAGA AAGTGATTGA AGTCCTGCAG GCCAAGCTAG ATGCTCGGTC TCTCACACCC 
TCCAGCAGCC ATGCATTGTC AGACTCCCAC CGCTCTCCCA GCAGCTCGTC CTTCCTGTCT 
GATGAGCTGG AAGCCTGCTC TGACATGGAT GTAGCCAGCG AGTACACACA TTATGAAGAG 
AAGAAGGCTC CACCTGGTCA CTCAGATTCC ATCCATCATT TGAGTCATTC TGCTGTACTG 
TCTTCTAAAC CATCAGCAAC CAGTGTACCT CGGGGGGTTA AGGCCGAACC CAGCAGCAAC 
CCCATCAGCT TGCCAGCTCC CCAGAACCCC CCCCAAGAGG CCAGCCAGGC CCATCCAGGC 
CTTCATTTTC CCTCCATATC CACACTGGTT AGCCTTCCCC AGGCACCACT GCCCTCAGCT 
CCACCCAGCT TCCTGCCTTT CAGCTCCACT GGCCCTCCCC TCCTTGGCTG CTGTGAGACA 
CCTATGGTCT CCTTGGCAGA GGCTCAGCAG GAGCTGCAGA TGCTGCAGAA ACAATTGGGA 
GAAAGTGTCA GCACCGTCCA TCCTGCTTCC CCAGCTGCAT TGCCAAGCAA CCATTTAGAA 
GCCAACTCTT CCCAATATCT CAACCCTGCC CAGCCCCACT CTCCTCCAAC TGGCAGCACA 
GAATTGGGCA GAATCCTGGA GCCTGGCTAC CTGGGTAGCG GCAGCCAGTG GGATGTGATG 
AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC TTATCCTCAG GATCCTCTGT GTGTCAGCTT 
AACTCCAAAC CCACAGGGGC TGACCTGCTA GAAGAACATC TTGGTGAAAT CCGGAACCTG 
CGCCAGCGCC TAGAGGAGTC CATCTGCATC AATGACCGCC TGCGGGAACA GCTGGAACAC 
CGGCTCAGCT GCACAGCACG TGGCAATGGA TCCACCTCTA ATTTCTACAG TCCTGGCCTG 
GAACCCACAC CTCAGCTCTA CAATGAGAAC AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT 
CAGGCTCAGC TGAGTCATGT TTCTAGAGAG CGCTCCCAGG AGACAGAATG CCTGAGGGAG 
GCTCTACTGT CCTCTCAATC CCGCCTTCAA GAGCTGGAAA TGGAGCTAGA GCACCAAAAG 
GAGGAACGGC AGCAGCTTTT GGAAGACTTG AAGGAGAAGC AGCAGGAGAT CTTGCACTTC 
CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA GACTCTAGGC TGCAGCGTAA GCTGGCCCTA 
CTGCAGCAAC AGTGTGAAGA GAAACAGCAG CTCTTCCAGT CCCTCCAGTC AGAGCTACAG 
ATCTATGAGG CGCTTTATGG CAGTTCCAAG AAGGGTCTGA AAGCTTCCAC TTGGGATGCC 
TATCACCAGG TCCCTTTGAG CAGTGACTTG AGCCACCTGG TGGCAGAGAT CCGAGCTTTG 
CGAGGGCAGC TGGAGCAAAG CATACAGGTG AATAACTGTC TGCGACTGCA GCTGGAGCAG 
CAGCTGGATG GCGGAAGGAG CAAAACCAGC CTCAGTCCCT CTTCCACCAG CCAGGAGGAC 
CCTGGAGACA AGCAAGACTC AATTGCTTCC CCTCCAGTCC GAGATGTTGG CATGAATTCT 
CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT GCTGCTCCAG GCTCAGAGAC AGCTATTTTC 
AATAGGATAA ATGAGCTGAA TTTGGATGCT TTTCCAGTAA TGAAGAACTC TCCCAAGCTG 
GAGGGCGAAG CTATCGATGG CTCCTTTGCC AATAAGCACG GCCGCCATGT GATTGGCCAC 
ATTGATGACT ATAGTGCCCT AAGACAGCAG ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG 
ATAACAGCAC TTGTGAGATC AGCCTACACC CTCCCTGGCC TTGAAACTCA GGCGACAGAG 
GTGCCAGGTG GCAAAGGCAT CCATGAACTT GGGAGCAGCA CCCAAGCCCT GCAGCACGTG 
CTGGAGGAGT CGGCCTCCCT CCTCAATATG TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC 
CCTGCCCTGT CTGGCAACAT GGGACAGGCC ATGAAAAAGG AACTTCTGGA ACTGAGAACC 
AAACTGACCA AACAGGAGAC CCTCCTTCAG TGCACAGCTG AGCGTCTAAA GACCACCCAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCATT TGACCAGGAC ACATGATGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGGCC AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC 
ACTTACAAGA TGGCCTCTGT AAAGCCTTAT TCCTGCCCCA GCAAAGGAGC CACGCAAGAA 
GAGGAGCCCC CAGAGGTCCT TCAAACAGCA GGAAGGATGG GCCTGCCCCC CTTTTGTGCA 
GGTCCCAATC TGCTGAGGAG GCTTGGACCC CACTCCTCCG CACTGGCTGG AGGGTCCAGG 
AGGAGTCAGG CATGGCCCAG TGGACGAGGG GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630749.1
CDS	16..7275
Translation	MFLCQGFRKYLPEHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630692.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630692.3

ORF Insert Sequence:

ATGTTCCTCT GTCAGGGATT CCGCAAATAT CTCCCGGAGC ATCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAT TTCCTGGAGG AACGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGAGGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGGG AACTCCAGGA CAAGAAACAG CATCTAGATA AAACGTGGGC TGATGTGGAG 
AATCTCAACA GCCATAATGA AGCTGAGCTC CGGCGTCAGT TTGAAGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAGGA GGAATCCAGG 
CTAGCAAAGA ATGAGGCTGC ACGGATGGCA GCTCTGGTGG AAGCTGAGAA AGAATGTAAC 
CTGGAGCTAT CAGAGAAACT GAAGGGAGTT GCCAATGACA GGGAAGAGGT ACCAATGGAC 
CAGGTCAATC CTGACCAATA CACTGAGGCC CTGGCCCAGC GGGACAAAAG AATTGAAGAA 
CTTCATCAAA GCCTGGCTTC CCAGGAGAAG CTTGTAGAAC AGCTGTCTCA AGAGAAACAA 
CAACTGCTCC ATCTGTTGGA GGAACCAATG AGCATGGAAA TACAGCCTGT GACTGAAGAG 
TTACTCAAGC AACAAAAGCT GGATTCAGAT GAGACCATTA TAACTCAGCA GTCTGTATCT 
GATTCCCACT TGGCTGAACT CCAGGAAAAA ATCCAGCAAA TGGAGGCCAC CAACAAAATT 
CTTCAAGAGA AACTGAATGA AATGAGCTGT GAGCTAAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG GTACAATTCA AAGCCTCAAG GAAACTCTGA AAAGTAGGGA AAATGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AATGACACAA TGGCAAAGCT TCGAGAAATG 
CTACACCAAA GCCAGCTTGG ACAACTTCAT GTATTTGAGG GTCACATAGG ACAGGAGAGC 
TCACAGGGTA CTTCCCCAGC TCAGCAACAG ATGGCTCTGC TTGATCTTCA GAGTGGTTTG 
TTCTACAGCC AGCTTGAGAT TCAGAAACTC CAGAGGTTGG TACGTCAAAA AGAACGCCAA 
CTGGCTGATG CCAAACGATG TGTCCAATTT GTAGAGGCTG CAGCCCATGA AAGAGAACAG 
CAGAAAGAGG CATCTTGGAA ACATAATCAG GAATTACGGA AAGCCTTGCA GCAGCTCCAA 
GGAGAATTAG AGAGTAAGAG CCAACAGCTT CGTACCCTGG AGACTGAAAA ATGTAATGAG 
ATCAGAGCCC ATGAGCAACA CATTCAACAC CTAAACCATA GTCTGAGCCA CAAAGAGCAG 
CTGCTTCAGG AATTTCGGGA GCTCCTGCAG TATCGAGATA AGTCAGACAA AACCCTTGAA 
GCAAATGAAA TGTTGCTTGA GAAACTTCGG CAGCGAATAC AAGACCGAGA TGTTGCTTTG 
GAGCGGGCTA TAGATGAAAA GTTCTCCGCC CTGGAAGAGA AAGAAAAAGA ACTGCGTCAA 
TTTCATCTTG CTGTGCGAGA GCGTGATCAT GACTTAGAGA GATTGCGTGC TATTCTCTCC 
TCTAATGAAG CTACCATGCA GAGTATGGAG AGTCTCCTGA GGGCCAAAGG CCTAGAAGTA 
GAGCAGTTAT CAGCTACCTG TCAAAACCTC CAGTGGCTGA AAGAAGAAAA GGAAACCAAA 
TTTAGCCATT GGCAGAAGGA ACAAGAAAGT ATCATTCAGC AGTTACAGAC ATCTCTGCAT 
GACAGGAACA AAGAAGTGGA GGATCTGAGT GCCACCTTGT TCTGTAAGCT TGGACCTGGG 
CAGAGTGAGG TAGCAGAAGA GCTGTGCCAG CGCCTACAGC GAAAGGAAAG GATGCTACAG 
GAACTGCTAA CCGATCGAAA CAAACAAGCA GTGGAACACG AAATAGAGAT TCAAGGCCTG 
CTTCAGTCTA TGAGCACCAG GGAGCAGGAG AGCCAAGCCG CTGCAGGGAA GATGGTGCAA 
GCCCTTATGG AAAGAAATTC AGAACTACAG GCCCTGCGCC AGTATTTAGG AGGTAAAGAC 
TTCATGGTTT CCCAAGCACT CATCTCTAAC CAACCCACAG AAGTCACCTC CAACAGCCCT 
CGTCTTGGAG AGCAGACTGA TCAAGGTTCA GTGCATATAC CCTCCAAAGA TGATAGCATT 
TCCCTGACTG CCAAAGGGGA TACCAGTATA CCCAGGTCCT CATTAGGAGA CCAGGATACA 
GTTGCAGGGC TGGAAAAAGA ACTGAGTAAT GCCAAAGAGG AGCTTGAGCT CATGGCTAAG 
AAAGAACGAG AAAGTCGGAT GGAACTTTCT GCACTACAGT CCATGGTGGC CGTGCAAGAG 
GAAGAGCTGC AGGTGCAGGC TGCTGACATG GAATCCCTGA CCAGGAATAT ACAGATTAAA 
GAAGACCTCA TAAAGGACCT GCAGATGCAA CTGGTTGATC CTGAAGATAT ACCAGCTATG 
GAACGCCTGA CCCAAGAGGT CTTACTTCTT CGGGAAAAAG TTGCTTCAGT AGAATCACAG 
GGTCAGGAAA CGTTAGGAAA TCGAAGACAA CAATTGCTGC TAATGCTCGA AGGGCTGGTG 
GATGAACGGA GTCGGCTCAA TGAAGCTTTA CAAGCAGAGA GACAGCTGTA CAGCAGCCTG 
GTGAAGTTCC ATGCCCATCC AGAGAGCTCT GAGAGAGACC GAACTCTGCA GGTGGAACTA 
GAAGGGGCCC AGGTGATACG TGGTCGGCTA GAAGAAGTTC TTGGAAGAAG CCTGGAGCGC 
TTAAGCAGGC TGGAGACCCT GGCTGCCATT GGAGGTGCAG CTGCAGGGGA AGATACAGAA 
GATGCTAGCA CTGAGTTCAC GGACAGCATC GAGGAGGAGG CTGCACACCA TAATCACCAG 
CAACTCATCA AGGTGGCTTT GGAGAAAAGT CTGGCAACTG TGGAGACTCA GAACGTATGT 
CTCCCCCCTC CTTCCTCAGC AGGAGGGGAC AATAACAGGG GTCTTCAGGA GGAGATGCTC 
CACCTGAGGG CTGAGATCCA CCAGCACTTA GAGGAGAAGA GGAGAGCTGA GGGGGAACTG 
AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA GGATTCTCCT CTGTGGCCCA CATCAGGAAC 
ACCATGCTGA GCCTTTGCCT TGAGAATGCA GAGCTGAAAG AGCAGATGGG AGAAGCAATG 
TCTGATGGAT GGGAGATCGA GGAAGACAAG GAGAAGGGCG AGGTGACAGT GGAAACTGTG 
GCAGCCAAAG GAGGTCTGAA TGAAAATAGC CTTCAGGCTG AGTTCAGAAA GCTCCAGGGA 
AAACTGAAGC ATGCCCACAA CATTATCAAC CTCCTCAAAG AGCAGCTGGT GCTGAGCAGC 
AAGGATGGGA ATAGTAAACT TAGTCCAGAG CTCCTAGTGC GCCTGGACCG GGAGATCGAC 
AGAATGAACA CAGAGCTGGT CTGTTCTCCG GAGAAGCCCC AGTGCCAGGA AGAAGAGCAT 
ATGACCACAA GGCCCTGCCC CAGACCCCAG AGCCTTGACC TTGGGGCTAC CCTCTCAGTG 
GATGGCCACC AACTGGATAA CCGGCCCCAG GCCCAAGACC CTGGGCCTCA GTCAGAATTT 
AGCCTTCCTG GATCCACCAA GCACCTGCGC TCCCAGCTGG CCCAATGCAG ACAACGCTAT 
CAGGATCTCC AGGAGAAGCT GCTGATATCG GAAGCCACAG TCTTTGCACA GGCCAACCAA 
CTGGAGAAAT ACCGAGTCAT GCTTAGTGAA TCCTTGGTGA AGCAGGACAG CAAGCAGGTG 
CAGGTGGACC TCCAGGATCT GGGCTATGAA ACCTGTGGCC GAAGTGAGAA TGAGGCTGAG 
CGGGAGGAGA GCACCAGTCC CGAGTGTGAG GAGCCTGACA GCCTCAGGGA AACAGTCCTG 
ATGGTGGGGC ACCTGGGCTC ACTGCTGGCC AGCCCTCCAG GGAAGAAGCC CTTGGAAAGC 
CATCGGGGGA AGCAGGAGGA GTTCCAGGCA TATGGAAAGT CAGAAGACAT CTCTGTCCTA 
CATAAGGACA TCAGAGATCT GAAGGCCCAG CTGCAGAATG CCAACAAGAT CATTCAGAAT 
CTTAAGAGCC GGGTCCGGTC CCTCTCAGTT ACAAGTGATT ATTCATCTAG TCTGGAGAGA 
CCAAGAAAGC TGAAGGCCAT TGGCACCCTT GAGGGATCCT CACCTCACAG TGTCACTGAC 
GAGGATGAGG GGTGGCTGTC TGATGGCACT GGGGCTTTCT GCACCCCAGG GCTTCAGGCT 
AAAAAGGATC TGGAGAGTCT GATCCAGAGA GTGTCTCAGC TGGAGGCCCA GCTCCCGAAA 
ACTGGAGTGG AAGGGAAGCT AGCAGAGGAG CTGAGATCAG CCTCATGGCC TGGGAAATAT 
GATTCCCTAA TTCAGGATCA GGCCCGAGAG CTGTCGTACC TACGCCAGAA GATACGAGAA 
GGGAGAGGTA TCTGTTATCT TCTCACCCAG CATGCAAAAG ATACAGTAAA ATCTTTTGAG 
GACCTTCTTA GGAGCAATGA CATTGACTAC TACCTGGGCC AGAGCTTCCG TGAGCAACTT 
GCCCAGGGAA GCCAGCTGAC AGAGAGACTC ACCAGTAAAC TCAGCACCAA GGATCATAAA 
AGTGAGAAAG ATCAAGCTGG ACTTGAACCA CTGGCCCTCA GGCTCAGCAG GGAGCTGCAG 
GAGAAGGAGA AAGTGATTGA AGTCCTGCAG GCCAAGCTAG ATGCTCGGTC TCTCACACCC 
TCCAGCAGCC ATGCATTGTC AGACTCCCAC CGCTCTCCCA GCAGCTCGTC CTTCCTGTCT 
GATGAGCTGG AAGCCTGCTC TGACATGGAT GTAGCCAGCG AGTACACACA TTATGAAGAG 
AAGAAGGCTC CACCTGGTCA CTCAGATTCC ATCCATCATT TGAGTCATTC TGCTGTACTG 
TCTTCTAAAC CATCAGCAAC CAGTGTACCT CGGGGGGTTA AGGCCGAACC CAGCAGCAAC 
CCCATCAGCT TGCCAGCTCC CCAGAACCCC CCCCAAGAGG CCAGCCAGGC CCATCCAGGC 
CTTCATTTTC CCTCCATATC CACACTGGTT AGCCTTCCCC AGGCACCACT GCCCTCAGCT 
CCACCCAGCT TCCTGCCTTT CAGCTCCACT GGCCCTCCCC TCCTTGGCTG CTGTGAGACA 
CCTATGGTCT CCTTGGCAGA GGCTCAGCAG GAGCTGCAGA TGCTGCAGAA ACAATTGGGA 
GAAAGTGTCA GCACCGTCCA TCCTGCTTCC CCAGCTGCAT TGCCAAGCAA CCATTTAGAA 
GCCAACTCTT CCCAATATCT CAACCCTGCC CAGCCCCACT CTCCTCCAAC TGGCAGCACA 
GAATTGGGCA GAATCCTGGA GCCTGGCTAC CTGGGTAGCG GCAGCCAGTG GGATGTGATG 
AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC TTATCCTCAG GATCCTCTGT GTGTCAGCTT 
AACTCCAAAC CCACAGGGGC TGACCTGCTA GAAGAACATC TTGGTGAAAT CCGGAACCTG 
CGCCAGCGCC TAGAGGAGTC CATCTGCATC AATGACCGCC TGCGGGAACA GCTGGAACAC 
CGGCTCAGCT GCACAGCACG TGGCAATGGA TCCACCTCTA ATTTCTACAG TCCTGGCCTG 
GAACCCACAC CTCAGCTCTA CAATGAGAAC AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT 
CAGGCTCAGC TGAGTCATGT TTCTAGAGAG CGCTCCCAGG AGACAGAATG CCTGAGGGAG 
GCTCTACTGT CCTCTCAATC CCGCCTTCAA GAGCTGGAAA TGGAGCTAGA GCACCAAAAG 
GAGGAACGGC AGCAGCTTTT GGAAGACTTG AAGGAGAAGC AGCAGGAGAT CTTGCACTTC 
CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA GACTCTAGGC TGCAGCGTAA GCTGGCCCTA 
CTGCAGCAAC AGTGTGAAGA GAAACAGCAG CTCTTCCAGT CCCTCCAGTC AGAGCTACAG 
ATCTATGAGG CGCTTTATGG CAGTTCCAAG AAGGGTCTGA AAGCTTCCAC TTGGGATGCC 
TATCACCAGG TCCCTTTGAG CAGTGACTTG AGCCACCTGG TGGCAGAGAT CCGAGCTTTG 
CGAGGGCAGC TGGAGCAAAG CATACAGGTG AATAACTGTC TGCGACTGCA GCTGGAGCAG 
CAGCTGGATG GCGGAAGGAG CAAAACCAGC CTCAGTCCCT CTTCCACCAG CCAGGAGGAC 
CCTGGAGACA AGCAAGACTC AATTGCTTCC CCTCCAGTCC GAGATGTTGG CATGAATTCT 
CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT GCTGCTCCAG GCTCAGAGAC AGCTATTTTC 
AATAGGATAA ATGAGCTGAA TTTGGATGCT TTTCCAGTAA TGAAGAACTC TCCCAAGCTG 
GAGGGCGAAG CTATCGATGG CTCCTTTGCC AATAAGCACG GCCGCCATGT GATTGGCCAC 
ATTGATGACT ATAGTGCCCT AAGACAGCAG ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG 
ATAACAGCAC TTGTGAGATC AGCCTACACC CTCCCTGGCC TTGAAACTCA GGCGACAGAG 
GTGCCAGGTG GCAAAGGCAT CCATGAACTT GGGAGCAGCA CCCAAGCCCT GCAGCACGTG 
CTGGAGGAGT CGGCCTCCCT CCTCAATATG TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC 
CCTGCCCTGT CTGGCAACAT GGGACAGGCC ATGAAAAAGG AACTTCTGGA ACTGAGAACC 
AAACTGACCA AACAGGAGAC CCTCCTTCAG TGCACAGCTG AGCGTCTAAA GACCACCCAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCATT TGACCAGGAC ACATGATGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGGCC AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC 
ACTTACAAGA TGGCCTCTGT AAAGCCTTAT TCCTGCCCCA GCAAAGGAGC CACGCAAGAA 
GAGGAGCCCC CAGAGGTCCT TCAAACAGCA GGAAGGATGG GCCTGCCCCC CTTTTGTGCA 
GGTCCCAATC TGCTGAGGAG GCTTGGACCC CACTCCTCCG CACTGGCTGG AGGGTCCAGG 
AGGAGTCAGG CATGGCCCAG TGGACGAGGG GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630689.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630746.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X2, mRNA.

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CloneID	OCe19356
Clone ID Related Accession (Same CDS sequence)	XM_005630689.3 , XM_005630689.2
Accession Version	XM_005630689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAATTGAA GAACTTCATC AAAGCCTGGC TTCCCAGGAG 
AAGCTTGTAG AACAGCTGTC TCAAGAGAAA CAACAACTGC TCCATCTGTT GGAGGAACCA 
ATGAGCATGG AAATACAGCC TGTGACTGAA GAGTTACTCA AGCAACAAAA GCTGGATTCA 
GATGAGACCA TTATAACTCA GCAGTCTGTA TCTGATTCCC ACTTGGCTGA ACTCCAGGAA 
AAAATCCAGC AAATGGAGGC CACCAACAAA ATTCTTCAAG AGAAACTGAA TGAAATGAGC 
TGTGAGCTAA AGTCTGCTCA GGAGTCATCT CAGAAGCAAG ATGGTACAAT TCAAAGCCTC 
AAGGAAACTC TGAAAAGTAG GGAAAATGAG ACCGAGGAGT TGTACCAGGT GATTGAAGGT 
CAAAATGACA CAATGGCAAA GCTTCGAGAA ATGCTACACC AAAGCCAGCT TGGACAACTT 
CATGTATTTG AGGGTCACAT AGGACAGGAG AGCTCACAGG GTACTTCCCC AGCTCAGCAA 
CAGATGGCTC TGCTTGATCT TCAGAGTGGT TTGTTCTACA GCCAGCTTGA GATTCAGAAA 
CTCCAGAGGT TGGTACGTCA AAAAGAACGC CAACTGGCTG ATGCCAAACG ATGTGTCCAA 
TTTGTAGAGG CTGCAGCCCA TGAAAGAGAA CAGCAGAAAG AGGCATCTTG GAAACATAAT 
CAGGAATTAC GGAAAGCCTT GCAGCAGCTC CAAGGAGAAT TAGAGAGTAA GAGCCAACAG 
CTTCGTACCC TGGAGACTGA AAAATGTAAT GAGATCAGAG CCCATGAGCA ACACATTCAA 
CACCTAAACC ATAGTCTGAG CCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTATCGAG ATAAGTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
CGGCAGCGAA TACAAGACCG AGATGTTGCT TTGGAGCGGG CTATAGATGA AAAGTTCTCC 
GCCCTGGAAG AGAAAGAAAA AGAACTGCGT CAATTTCATC TTGCTGTGCG AGAGCGTGAT 
CATGACTTAG AGAGATTGCG TGCTATTCTC TCCTCTAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGGCCAA AGGCCTAGAA GTAGAGCAGT TATCAGCTAC CTGTCAAAAC 
CTCCAGTGGC TGAAAGAAGA AAAGGAAACC AAATTTAGCC ATTGGCAGAA GGAACAAGAA 
AGTATCATTC AGCAGTTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTG 
AGTGCCACCT TGTTCTGTAA GCTTGGACCT GGGCAGAGTG AGGTAGCAGA AGAGCTGTGC 
CAGCGCCTAC AGCGAAAGGA AAGGATGCTA CAGGAACTGC TAACCGATCG AAACAAACAA 
GCAGTGGAAC ACGAAATAGA GATTCAAGGC CTGCTTCAGT CTATGAGCAC CAGGGAGCAG 
GAGAGCCAAG CCGCTGCAGG GAAGATGGTG CAAGCCCTTA TGGAAAGAAA TTCAGAACTA 
CAGGCCCTGC GCCAGTATTT AGGAGGTAAA GACTTCATGG TTTCCCAAGC ACTCATCTCT 
AACCAACCCA CAGAAGTCAC CTCCAACAGC CCTCGTCTTG GAGAGCAGAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAA AGATGATAGC ATTTCCCTGA CTGCCAAAGG GGATACCAGT 
ATACCCAGGT CCTCATTAGG AGACCAGGAT ACAGTTGCAG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAGCTTGA GCTCATGGCT AAGAAAGAAC GAGAAAGTCG GATGGAACTT 
TCTGCACTAC AGTCCATGGT GGCCGTGCAA GAGGAAGAGC TGCAGGTGCA GGCTGCTGAC 
ATGGAATCCC TGACCAGGAA TATACAGATT AAAGAAGACC TCATAAAGGA CCTGCAGATG 
CAACTGGTTG ATCCTGAAGA TATACCAGCT ATGGAACGCC TGACCCAAGA GGTCTTACTT 
CTTCGGGAAA AAGTTGCTTC AGTAGAATCA CAGGGTCAGG AAACGTTAGG AAATCGAAGA 
CAACAATTGC TGCTAATGCT CGAAGGGCTG GTGGATGAAC GGAGTCGGCT CAATGAAGCT 
TTACAAGCAG AGAGACAGCT GTACAGCAGC CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGTGGTCGG 
CTAGAAGAAG TTCTTGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCTGCC 
ATTGGAGGTG CAGCTGCAGG GGAAGATACA GAAGATGCTA GCACTGAGTT CACGGACAGC 
ATCGAGGAGG AGGCTGCACA CCATAATCAC CAGCAACTCA TCAAGGTGGC TTTGGAGAAA 
AGTCTGGCAA CTGTGGAGAC TCAGAACGTA TGTCTCCCCC CTCCTTCCTC AGCAGGAGGG 
GACAATAACA GGGGTCTTCA GGAGGAGATG CTCCACCTGA GGGCTGAGAT CCACCAGCAC 
TTAGAGGAGA AGAGGAGAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT CGAGGAAGAC 
AAGGAGAAGG GCGAGGTGAC AGTGGAAACT GTGGCAGCCA AAGGAGGTCT GAATGAAAAT 
AGCCTTCAGG CTGAGTTCAG AAAGCTCCAG GGAAAACTGA AGCATGCCCA CAACATTATC 
AACCTCCTCA AAGAGCAGCT GGTGCTGAGC AGCAAGGATG GGAATAGTAA ACTTAGTCCA 
GAGCTCCTAG TGCGCCTGGA CCGGGAGATC GACAGAATGA ACACAGAGCT GGTCTGTTCT 
CCGGAGAAGC CCCAGTGCCA GGAAGAAGAG CATATGACCA CAAGGCCCTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TACCCTCTCA GTGGATGGCC ACCAACTGGA TAACCGGCCC 
CAGGCCCAAG ACCCTGGGCC TCAGTCAGAA TTTAGCCTTC CTGGATCCAC CAAGCACCTG 
CGCTCCCAGC TGGCCCAATG CAGACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCGGAAGCCA CAGTCTTTGC ACAGGCCAAC CAACTGGAGA AATACCGAGT CATGCTTAGT 
GAATCCTTGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA TCTGGGCTAT 
GAAACCTGTG GCCGAAGTGA GAATGAGGCT GAGCGGGAGG AGAGCACCAG TCCCGAGTGT 
GAGGAGCCTG ACAGCCTCAG GGAAACAGTC CTGATGGTGG GGCACCTGGG CTCACTGCTG 
GCCAGCCCTC CAGGGAAGAA GCCCTTGGAA AGCCATCGGG GGAAGCAGGA GGAGTTCCAG 
GCATATGGAA AGTCAGAAGA CATCTCTGTC CTACATAAGG ACATCAGAGA TCTGAAGGCC 
CAGCTGCAGA ATGCCAACAA GATCATTCAG AATCTTAAGA GCCGGGTCCG GTCCCTCTCA 
GTTACAAGTG ATTATTCATC TAGTCTGGAG AGACCAAGAA AGCTGAAGGC CATTGGCACC 
CTTGAGGGAT CCTCACCTCA CAGTGTCACT GACGAGGATG AGGGGTGGCT GTCTGATGGC 
ACTGGGGCTT TCTGCACCCC AGGGCTTCAG GCTAAAAAGG ATCTGGAGAG TCTGATCCAG 
AGAGTGTCTC AGCTGGAGGC CCAGCTCCCG AAAACTGGAG TGGAAGGGAA GCTAGCAGAG 
GAGCTGAGAT CAGCCTCATG GCCTGGGAAA TATGATTCCC TAATTCAGGA TCAGGCCCGA 
GAGCTGTCGT ACCTACGCCA GAAGATACGA GAAGGGAGAG GTATCTGTTA TCTTCTCACC 
CAGCATGCAA AAGATACAGT AAAATCTTTT GAGGACCTTC TTAGGAGCAA TGACATTGAC 
TACTACCTGG GCCAGAGCTT CCGTGAGCAA CTTGCCCAGG GAAGCCAGCT GACAGAGAGA 
CTCACCAGTA AACTCAGCAC CAAGGATCAT AAAAGTGAGA AAGATCAAGC TGGACTTGAA 
CCACTGGCCC TCAGGCTCAG CAGGGAGCTG CAGGAGAAGG AGAAAGTGAT TGAAGTCCTG 
CAGGCCAAGC TAGATGCTCG GTCTCTCACA CCCTCCAGCA GCCATGCATT GTCAGACTCC 
CACCGCTCTC CCAGCAGCTC GTCCTTCCTG TCTGATGAGC TGGAAGCCTG CTCTGACATG 
GATGTAGCCA GCGAGTACAC ACATTATGAA GAGAAGAAGG CTCCACCTGG TCACTCAGAT 
TCCATCCATC ATTTGAGTCA TTCTGCTGTA CTGTCTTCTA AACCATCAGC AACCAGTGTA 
CCTCGGGGGG TTAAGGCCGA ACCCAGCAGC AACCCCATCA GCTTGCCAGC TCCCCAGAAC 
CCCCCCCAAG AGGCCAGCCA GGCCCATCCA GGCCTTCATT TTCCCTCCAT ATCCACACTG 
GTTAGCCTTC CCCAGGCACC ACTGCCCTCA GCTCCACCCA GCTTCCTGCC TTTCAGCTCC 
ACTGGCCCTC CCCTCCTTGG CTGCTGTGAG ACACCTATGG TCTCCTTGGC AGAGGCTCAG 
CAGGAGCTGC AGATGCTGCA GAAACAATTG GGAGAAAGTG TCAGCACCGT CCATCCTGCT 
TCCCCAGCTG CATTGCCAAG CAACCATTTA GAAGCCAACT CTTCCCAATA TCTCAACCCT 
GCCCAGCCCC ACTCTCCTCC AACTGGCAGC ACAGAATTGG GCAGAATCCT GGAGCCTGGC 
TACCTGGGTA GCGGCAGCCA GTGGGATGTG ATGAGGCCTC AGAAGGGGAG TGTATCAGGC 
GACTTATCCT CAGGATCCTC TGTGTGTCAG CTTAACTCCA AACCCACAGG GGCTGACCTG 
CTAGAAGAAC ATCTTGGTGA AATCCGGAAC CTGCGCCAGC GCCTAGAGGA GTCCATCTGC 
ATCAATGACC GCCTGCGGGA ACAGCTGGAA CACCGGCTCA GCTGCACAGC ACGTGGCAAT 
GGATCCACCT CTAATTTCTA CAGTCCTGGC CTGGAACCCA CACCTCAGCT CTACAATGAG 
AACAGAGTCC TTAAGGAAGA AAACCGGAGC CTTCAGGCTC AGCTGAGTCA TGTTTCTAGA 
GAGCGCTCCC AGGAGACAGA ATGCCTGAGG GAGGCTCTAC TGTCCTCTCA ATCCCGCCTT 
CAAGAGCTGG AAATGGAGCT AGAGCACCAA AAGGAGGAAC GGCAGCAGCT TTTGGAAGAC 
TTGAAGGAGA AGCAGCAGGA GATCTTGCAC TTCCAGGAGG AGCGCCTGTC CCTCCAGGAG 
AAAGACTCTA GGCTGCAGCG TAAGCTGGCC CTACTGCAGC AACAGTGTGA AGAGAAACAG 
CAGCTCTTCC AGTCCCTCCA GTCAGAGCTA CAGATCTATG AGGCGCTTTA TGGCAGTTCC 
AAGAAGGGTC TGAAAGCTTC CACTTGGGAT GCCTATCACC AGGTCCCTTT GAGCAGTGAC 
TTGAGCCACC TGGTGGCAGA GATCCGAGCT TTGCGAGGGC AGCTGGAGCA AAGCATACAG 
GTGAATAACT GTCTGCGACT GCAGCTGGAG CAGCAGCTGG ATGGCGGAAG GAGCAAAACC 
AGCCTCAGTC CCTCTTCCAC CAGCCAGGAG GACCCTGGAG ACAAGCAAGA CTCAATTGCT 
TCCCCTCCAG TCCGAGATGT TGGCATGAAT TCTCCAGCTC TCATGTTCCC TAGCTCTTCT 
CCTGCTGCTC CAGGCTCAGA GACAGCTATT TTCAATAGGA TAAATGAGCT GAATTTGGAT 
GCTTTTCCAG TAATGAAGAA CTCTCCCAAG CTGGAGGGCG AAGCTATCGA TGGCTCCTTT 
GCCAATAAGC ACGGCCGCCA TGTGATTGGC CACATTGATG ACTATAGTGC CCTAAGACAG 
CAGATTGGTG AGGGCAAAGT GCTGGTCAAA AAGATAACAG CACTTGTGAG ATCAGCCTAC 
ACCCTCCCTG GCCTTGAAAC TCAGGCGACA GAGGTGCCAG GTGGCAAAGG CATCCATGAA 
CTTGGGAGCA GCACCCAAGC CCTGCAGCAC GTGCTGGAGG AGTCGGCCTC CCTCCTCAAT 
ATGTTCTGGC GAGCGGCCCT GCCAAGCTCC AGCCCTGCCC TGTCTGGCAA CATGGGACAG 
GCCATGAAAA AGGAACTTCT GGAACTGAGA ACCAAACTGA CCAAACAGGA GACCCTCCTT 
CAGTGCACAG CTGAGCGTCT AAAGACCACC CACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC ATTTGACCAG GACACATGAT GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
GCCAAGTCAG GAATCCGAGG AAAGCAAAAG AACACTTACA AGATGGCCTC TGTAAAGCCT 
TATTCCTGCC CCAGCAAAGG AGCCACGCAA GAAGAGGAGC CCCCAGAGGT CCTTCAAACA 
GCAGGAAGGA TGGGCCTGCC CCCCTTTTGT GCAGGTCCCA ATCTGCTGAG GAGGCTTGGA 
CCCCACTCCT CCGCACTGGC TGGAGGGTCC AGGAGGAGTC AGGCATGGCC CAGTGGACGA 
GGGGGAAAGG AAGGCAGCCT TTCCTGGGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630746.1
CDS	16..7848
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630689.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630689.3

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAATTGAA GAACTTCATC AAAGCCTGGC TTCCCAGGAG 
AAGCTTGTAG AACAGCTGTC TCAAGAGAAA CAACAACTGC TCCATCTGTT GGAGGAACCA 
ATGAGCATGG AAATACAGCC TGTGACTGAA GAGTTACTCA AGCAACAAAA GCTGGATTCA 
GATGAGACCA TTATAACTCA GCAGTCTGTA TCTGATTCCC ACTTGGCTGA ACTCCAGGAA 
AAAATCCAGC AAATGGAGGC CACCAACAAA ATTCTTCAAG AGAAACTGAA TGAAATGAGC 
TGTGAGCTAA AGTCTGCTCA GGAGTCATCT CAGAAGCAAG ATGGTACAAT TCAAAGCCTC 
AAGGAAACTC TGAAAAGTAG GGAAAATGAG ACCGAGGAGT TGTACCAGGT GATTGAAGGT 
CAAAATGACA CAATGGCAAA GCTTCGAGAA ATGCTACACC AAAGCCAGCT TGGACAACTT 
CATGTATTTG AGGGTCACAT AGGACAGGAG AGCTCACAGG GTACTTCCCC AGCTCAGCAA 
CAGATGGCTC TGCTTGATCT TCAGAGTGGT TTGTTCTACA GCCAGCTTGA GATTCAGAAA 
CTCCAGAGGT TGGTACGTCA AAAAGAACGC CAACTGGCTG ATGCCAAACG ATGTGTCCAA 
TTTGTAGAGG CTGCAGCCCA TGAAAGAGAA CAGCAGAAAG AGGCATCTTG GAAACATAAT 
CAGGAATTAC GGAAAGCCTT GCAGCAGCTC CAAGGAGAAT TAGAGAGTAA GAGCCAACAG 
CTTCGTACCC TGGAGACTGA AAAATGTAAT GAGATCAGAG CCCATGAGCA ACACATTCAA 
CACCTAAACC ATAGTCTGAG CCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTATCGAG ATAAGTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
CGGCAGCGAA TACAAGACCG AGATGTTGCT TTGGAGCGGG CTATAGATGA AAAGTTCTCC 
GCCCTGGAAG AGAAAGAAAA AGAACTGCGT CAATTTCATC TTGCTGTGCG AGAGCGTGAT 
CATGACTTAG AGAGATTGCG TGCTATTCTC TCCTCTAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGGCCAA AGGCCTAGAA GTAGAGCAGT TATCAGCTAC CTGTCAAAAC 
CTCCAGTGGC TGAAAGAAGA AAAGGAAACC AAATTTAGCC ATTGGCAGAA GGAACAAGAA 
AGTATCATTC AGCAGTTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTG 
AGTGCCACCT TGTTCTGTAA GCTTGGACCT GGGCAGAGTG AGGTAGCAGA AGAGCTGTGC 
CAGCGCCTAC AGCGAAAGGA AAGGATGCTA CAGGAACTGC TAACCGATCG AAACAAACAA 
GCAGTGGAAC ACGAAATAGA GATTCAAGGC CTGCTTCAGT CTATGAGCAC CAGGGAGCAG 
GAGAGCCAAG CCGCTGCAGG GAAGATGGTG CAAGCCCTTA TGGAAAGAAA TTCAGAACTA 
CAGGCCCTGC GCCAGTATTT AGGAGGTAAA GACTTCATGG TTTCCCAAGC ACTCATCTCT 
AACCAACCCA CAGAAGTCAC CTCCAACAGC CCTCGTCTTG GAGAGCAGAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAA AGATGATAGC ATTTCCCTGA CTGCCAAAGG GGATACCAGT 
ATACCCAGGT CCTCATTAGG AGACCAGGAT ACAGTTGCAG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAGCTTGA GCTCATGGCT AAGAAAGAAC GAGAAAGTCG GATGGAACTT 
TCTGCACTAC AGTCCATGGT GGCCGTGCAA GAGGAAGAGC TGCAGGTGCA GGCTGCTGAC 
ATGGAATCCC TGACCAGGAA TATACAGATT AAAGAAGACC TCATAAAGGA CCTGCAGATG 
CAACTGGTTG ATCCTGAAGA TATACCAGCT ATGGAACGCC TGACCCAAGA GGTCTTACTT 
CTTCGGGAAA AAGTTGCTTC AGTAGAATCA CAGGGTCAGG AAACGTTAGG AAATCGAAGA 
CAACAATTGC TGCTAATGCT CGAAGGGCTG GTGGATGAAC GGAGTCGGCT CAATGAAGCT 
TTACAAGCAG AGAGACAGCT GTACAGCAGC CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGTGGTCGG 
CTAGAAGAAG TTCTTGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCTGCC 
ATTGGAGGTG CAGCTGCAGG GGAAGATACA GAAGATGCTA GCACTGAGTT CACGGACAGC 
ATCGAGGAGG AGGCTGCACA CCATAATCAC CAGCAACTCA TCAAGGTGGC TTTGGAGAAA 
AGTCTGGCAA CTGTGGAGAC TCAGAACGTA TGTCTCCCCC CTCCTTCCTC AGCAGGAGGG 
GACAATAACA GGGGTCTTCA GGAGGAGATG CTCCACCTGA GGGCTGAGAT CCACCAGCAC 
TTAGAGGAGA AGAGGAGAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT CGAGGAAGAC 
AAGGAGAAGG GCGAGGTGAC AGTGGAAACT GTGGCAGCCA AAGGAGGTCT GAATGAAAAT 
AGCCTTCAGG CTGAGTTCAG AAAGCTCCAG GGAAAACTGA AGCATGCCCA CAACATTATC 
AACCTCCTCA AAGAGCAGCT GGTGCTGAGC AGCAAGGATG GGAATAGTAA ACTTAGTCCA 
GAGCTCCTAG TGCGCCTGGA CCGGGAGATC GACAGAATGA ACACAGAGCT GGTCTGTTCT 
CCGGAGAAGC CCCAGTGCCA GGAAGAAGAG CATATGACCA CAAGGCCCTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TACCCTCTCA GTGGATGGCC ACCAACTGGA TAACCGGCCC 
CAGGCCCAAG ACCCTGGGCC TCAGTCAGAA TTTAGCCTTC CTGGATCCAC CAAGCACCTG 
CGCTCCCAGC TGGCCCAATG CAGACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCGGAAGCCA CAGTCTTTGC ACAGGCCAAC CAACTGGAGA AATACCGAGT CATGCTTAGT 
GAATCCTTGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA TCTGGGCTAT 
GAAACCTGTG GCCGAAGTGA GAATGAGGCT GAGCGGGAGG AGAGCACCAG TCCCGAGTGT 
GAGGAGCCTG ACAGCCTCAG GGAAACAGTC CTGATGGTGG GGCACCTGGG CTCACTGCTG 
GCCAGCCCTC CAGGGAAGAA GCCCTTGGAA AGCCATCGGG GGAAGCAGGA GGAGTTCCAG 
GCATATGGAA AGTCAGAAGA CATCTCTGTC CTACATAAGG ACATCAGAGA TCTGAAGGCC 
CAGCTGCAGA ATGCCAACAA GATCATTCAG AATCTTAAGA GCCGGGTCCG GTCCCTCTCA 
GTTACAAGTG ATTATTCATC TAGTCTGGAG AGACCAAGAA AGCTGAAGGC CATTGGCACC 
CTTGAGGGAT CCTCACCTCA CAGTGTCACT GACGAGGATG AGGGGTGGCT GTCTGATGGC 
ACTGGGGCTT TCTGCACCCC AGGGCTTCAG GCTAAAAAGG ATCTGGAGAG TCTGATCCAG 
AGAGTGTCTC AGCTGGAGGC CCAGCTCCCG AAAACTGGAG TGGAAGGGAA GCTAGCAGAG 
GAGCTGAGAT CAGCCTCATG GCCTGGGAAA TATGATTCCC TAATTCAGGA TCAGGCCCGA 
GAGCTGTCGT ACCTACGCCA GAAGATACGA GAAGGGAGAG GTATCTGTTA TCTTCTCACC 
CAGCATGCAA AAGATACAGT AAAATCTTTT GAGGACCTTC TTAGGAGCAA TGACATTGAC 
TACTACCTGG GCCAGAGCTT CCGTGAGCAA CTTGCCCAGG GAAGCCAGCT GACAGAGAGA 
CTCACCAGTA AACTCAGCAC CAAGGATCAT AAAAGTGAGA AAGATCAAGC TGGACTTGAA 
CCACTGGCCC TCAGGCTCAG CAGGGAGCTG CAGGAGAAGG AGAAAGTGAT TGAAGTCCTG 
CAGGCCAAGC TAGATGCTCG GTCTCTCACA CCCTCCAGCA GCCATGCATT GTCAGACTCC 
CACCGCTCTC CCAGCAGCTC GTCCTTCCTG TCTGATGAGC TGGAAGCCTG CTCTGACATG 
GATGTAGCCA GCGAGTACAC ACATTATGAA GAGAAGAAGG CTCCACCTGG TCACTCAGAT 
TCCATCCATC ATTTGAGTCA TTCTGCTGTA CTGTCTTCTA AACCATCAGC AACCAGTGTA 
CCTCGGGGGG TTAAGGCCGA ACCCAGCAGC AACCCCATCA GCTTGCCAGC TCCCCAGAAC 
CCCCCCCAAG AGGCCAGCCA GGCCCATCCA GGCCTTCATT TTCCCTCCAT ATCCACACTG 
GTTAGCCTTC CCCAGGCACC ACTGCCCTCA GCTCCACCCA GCTTCCTGCC TTTCAGCTCC 
ACTGGCCCTC CCCTCCTTGG CTGCTGTGAG ACACCTATGG TCTCCTTGGC AGAGGCTCAG 
CAGGAGCTGC AGATGCTGCA GAAACAATTG GGAGAAAGTG TCAGCACCGT CCATCCTGCT 
TCCCCAGCTG CATTGCCAAG CAACCATTTA GAAGCCAACT CTTCCCAATA TCTCAACCCT 
GCCCAGCCCC ACTCTCCTCC AACTGGCAGC ACAGAATTGG GCAGAATCCT GGAGCCTGGC 
TACCTGGGTA GCGGCAGCCA GTGGGATGTG ATGAGGCCTC AGAAGGGGAG TGTATCAGGC 
GACTTATCCT CAGGATCCTC TGTGTGTCAG CTTAACTCCA AACCCACAGG GGCTGACCTG 
CTAGAAGAAC ATCTTGGTGA AATCCGGAAC CTGCGCCAGC GCCTAGAGGA GTCCATCTGC 
ATCAATGACC GCCTGCGGGA ACAGCTGGAA CACCGGCTCA GCTGCACAGC ACGTGGCAAT 
GGATCCACCT CTAATTTCTA CAGTCCTGGC CTGGAACCCA CACCTCAGCT CTACAATGAG 
AACAGAGTCC TTAAGGAAGA AAACCGGAGC CTTCAGGCTC AGCTGAGTCA TGTTTCTAGA 
GAGCGCTCCC AGGAGACAGA ATGCCTGAGG GAGGCTCTAC TGTCCTCTCA ATCCCGCCTT 
CAAGAGCTGG AAATGGAGCT AGAGCACCAA AAGGAGGAAC GGCAGCAGCT TTTGGAAGAC 
TTGAAGGAGA AGCAGCAGGA GATCTTGCAC TTCCAGGAGG AGCGCCTGTC CCTCCAGGAG 
AAAGACTCTA GGCTGCAGCG TAAGCTGGCC CTACTGCAGC AACAGTGTGA AGAGAAACAG 
CAGCTCTTCC AGTCCCTCCA GTCAGAGCTA CAGATCTATG AGGCGCTTTA TGGCAGTTCC 
AAGAAGGGTC TGAAAGCTTC CACTTGGGAT GCCTATCACC AGGTCCCTTT GAGCAGTGAC 
TTGAGCCACC TGGTGGCAGA GATCCGAGCT TTGCGAGGGC AGCTGGAGCA AAGCATACAG 
GTGAATAACT GTCTGCGACT GCAGCTGGAG CAGCAGCTGG ATGGCGGAAG GAGCAAAACC 
AGCCTCAGTC CCTCTTCCAC CAGCCAGGAG GACCCTGGAG ACAAGCAAGA CTCAATTGCT 
TCCCCTCCAG TCCGAGATGT TGGCATGAAT TCTCCAGCTC TCATGTTCCC TAGCTCTTCT 
CCTGCTGCTC CAGGCTCAGA GACAGCTATT TTCAATAGGA TAAATGAGCT GAATTTGGAT 
GCTTTTCCAG TAATGAAGAA CTCTCCCAAG CTGGAGGGCG AAGCTATCGA TGGCTCCTTT 
GCCAATAAGC ACGGCCGCCA TGTGATTGGC CACATTGATG ACTATAGTGC CCTAAGACAG 
CAGATTGGTG AGGGCAAAGT GCTGGTCAAA AAGATAACAG CACTTGTGAG ATCAGCCTAC 
ACCCTCCCTG GCCTTGAAAC TCAGGCGACA GAGGTGCCAG GTGGCAAAGG CATCCATGAA 
CTTGGGAGCA GCACCCAAGC CCTGCAGCAC GTGCTGGAGG AGTCGGCCTC CCTCCTCAAT 
ATGTTCTGGC GAGCGGCCCT GCCAAGCTCC AGCCCTGCCC TGTCTGGCAA CATGGGACAG 
GCCATGAAAA AGGAACTTCT GGAACTGAGA ACCAAACTGA CCAAACAGGA GACCCTCCTT 
CAGTGCACAG CTGAGCGTCT AAAGACCACC CACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC ATTTGACCAG GACACATGAT GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
GCCAAGTCAG GAATCCGAGG AAAGCAAAAG AACACTTACA AGATGGCCTC TGTAAAGCCT 
TATTCCTGCC CCAGCAAAGG AGCCACGCAA GAAGAGGAGC CCCCAGAGGT CCTTCAAACA 
GCAGGAAGGA TGGGCCTGCC CCCCTTTTGT GCAGGTCCCA ATCTGCTGAG GAGGCTTGGA 
CCCCACTCCT CCGCACTGGC TGGAGGGTCC AGGAGGAGTC AGGCATGGCC CAGTGGACGA 
GGGGGAAAGG AAGGCAGCCT TTCCTGGGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630689.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630746.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X2, mRNA.

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CloneID	OCe19356
Clone ID Related Accession (Same CDS sequence)	XM_005630689.3 , XM_005630689.2
Accession Version	XM_005630689.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAATTGAA GAACTTCATC AAAGCCTGGC TTCCCAGGAG 
AAGCTTGTAG AACAGCTGTC TCAAGAGAAA CAACAACTGC TCCATCTGTT GGAGGAACCA 
ATGAGCATGG AAATACAGCC TGTGACTGAA GAGTTACTCA AGCAACAAAA GCTGGATTCA 
GATGAGACCA TTATAACTCA GCAGTCTGTA TCTGATTCCC ACTTGGCTGA ACTCCAGGAA 
AAAATCCAGC AAATGGAGGC CACCAACAAA ATTCTTCAAG AGAAACTGAA TGAAATGAGC 
TGTGAGCTAA AGTCTGCTCA GGAGTCATCT CAGAAGCAAG ATGGTACAAT TCAAAGCCTC 
AAGGAAACTC TGAAAAGTAG GGAAAATGAG ACCGAGGAGT TGTACCAGGT GATTGAAGGT 
CAAAATGACA CAATGGCAAA GCTTCGAGAA ATGCTACACC AAAGCCAGCT TGGACAACTT 
CATGTATTTG AGGGTCACAT AGGACAGGAG AGCTCACAGG GTACTTCCCC AGCTCAGCAA 
CAGATGGCTC TGCTTGATCT TCAGAGTGGT TTGTTCTACA GCCAGCTTGA GATTCAGAAA 
CTCCAGAGGT TGGTACGTCA AAAAGAACGC CAACTGGCTG ATGCCAAACG ATGTGTCCAA 
TTTGTAGAGG CTGCAGCCCA TGAAAGAGAA CAGCAGAAAG AGGCATCTTG GAAACATAAT 
CAGGAATTAC GGAAAGCCTT GCAGCAGCTC CAAGGAGAAT TAGAGAGTAA GAGCCAACAG 
CTTCGTACCC TGGAGACTGA AAAATGTAAT GAGATCAGAG CCCATGAGCA ACACATTCAA 
CACCTAAACC ATAGTCTGAG CCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTATCGAG ATAAGTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
CGGCAGCGAA TACAAGACCG AGATGTTGCT TTGGAGCGGG CTATAGATGA AAAGTTCTCC 
GCCCTGGAAG AGAAAGAAAA AGAACTGCGT CAATTTCATC TTGCTGTGCG AGAGCGTGAT 
CATGACTTAG AGAGATTGCG TGCTATTCTC TCCTCTAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGGCCAA AGGCCTAGAA GTAGAGCAGT TATCAGCTAC CTGTCAAAAC 
CTCCAGTGGC TGAAAGAAGA AAAGGAAACC AAATTTAGCC ATTGGCAGAA GGAACAAGAA 
AGTATCATTC AGCAGTTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTG 
AGTGCCACCT TGTTCTGTAA GCTTGGACCT GGGCAGAGTG AGGTAGCAGA AGAGCTGTGC 
CAGCGCCTAC AGCGAAAGGA AAGGATGCTA CAGGAACTGC TAACCGATCG AAACAAACAA 
GCAGTGGAAC ACGAAATAGA GATTCAAGGC CTGCTTCAGT CTATGAGCAC CAGGGAGCAG 
GAGAGCCAAG CCGCTGCAGG GAAGATGGTG CAAGCCCTTA TGGAAAGAAA TTCAGAACTA 
CAGGCCCTGC GCCAGTATTT AGGAGGTAAA GACTTCATGG TTTCCCAAGC ACTCATCTCT 
AACCAACCCA CAGAAGTCAC CTCCAACAGC CCTCGTCTTG GAGAGCAGAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAA AGATGATAGC ATTTCCCTGA CTGCCAAAGG GGATACCAGT 
ATACCCAGGT CCTCATTAGG AGACCAGGAT ACAGTTGCAG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAGCTTGA GCTCATGGCT AAGAAAGAAC GAGAAAGTCG GATGGAACTT 
TCTGCACTAC AGTCCATGGT GGCCGTGCAA GAGGAAGAGC TGCAGGTGCA GGCTGCTGAC 
ATGGAATCCC TGACCAGGAA TATACAGATT AAAGAAGACC TCATAAAGGA CCTGCAGATG 
CAACTGGTTG ATCCTGAAGA TATACCAGCT ATGGAACGCC TGACCCAAGA GGTCTTACTT 
CTTCGGGAAA AAGTTGCTTC AGTAGAATCA CAGGGTCAGG AAACGTTAGG AAATCGAAGA 
CAACAATTGC TGCTAATGCT CGAAGGGCTG GTGGATGAAC GGAGTCGGCT CAATGAAGCT 
TTACAAGCAG AGAGACAGCT GTACAGCAGC CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGTGGTCGG 
CTAGAAGAAG TTCTTGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCTGCC 
ATTGGAGGTG CAGCTGCAGG GGAAGATACA GAAGATGCTA GCACTGAGTT CACGGACAGC 
ATCGAGGAGG AGGCTGCACA CCATAATCAC CAGCAACTCA TCAAGGTGGC TTTGGAGAAA 
AGTCTGGCAA CTGTGGAGAC TCAGAACGTA TGTCTCCCCC CTCCTTCCTC AGCAGGAGGG 
GACAATAACA GGGGTCTTCA GGAGGAGATG CTCCACCTGA GGGCTGAGAT CCACCAGCAC 
TTAGAGGAGA AGAGGAGAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT CGAGGAAGAC 
AAGGAGAAGG GCGAGGTGAC AGTGGAAACT GTGGCAGCCA AAGGAGGTCT GAATGAAAAT 
AGCCTTCAGG CTGAGTTCAG AAAGCTCCAG GGAAAACTGA AGCATGCCCA CAACATTATC 
AACCTCCTCA AAGAGCAGCT GGTGCTGAGC AGCAAGGATG GGAATAGTAA ACTTAGTCCA 
GAGCTCCTAG TGCGCCTGGA CCGGGAGATC GACAGAATGA ACACAGAGCT GGTCTGTTCT 
CCGGAGAAGC CCCAGTGCCA GGAAGAAGAG CATATGACCA CAAGGCCCTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TACCCTCTCA GTGGATGGCC ACCAACTGGA TAACCGGCCC 
CAGGCCCAAG ACCCTGGGCC TCAGTCAGAA TTTAGCCTTC CTGGATCCAC CAAGCACCTG 
CGCTCCCAGC TGGCCCAATG CAGACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCGGAAGCCA CAGTCTTTGC ACAGGCCAAC CAACTGGAGA AATACCGAGT CATGCTTAGT 
GAATCCTTGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA TCTGGGCTAT 
GAAACCTGTG GCCGAAGTGA GAATGAGGCT GAGCGGGAGG AGAGCACCAG TCCCGAGTGT 
GAGGAGCCTG ACAGCCTCAG GGAAACAGTC CTGATGGTGG GGCACCTGGG CTCACTGCTG 
GCCAGCCCTC CAGGGAAGAA GCCCTTGGAA AGCCATCGGG GGAAGCAGGA GGAGTTCCAG 
GCATATGGAA AGTCAGAAGA CATCTCTGTC CTACATAAGG ACATCAGAGA TCTGAAGGCC 
CAGCTGCAGA ATGCCAACAA GATCATTCAG AATCTTAAGA GCCGGGTCCG GTCCCTCTCA 
GTTACAAGTG ATTATTCATC TAGTCTGGAG AGACCAAGAA AGCTGAAGGC CATTGGCACC 
CTTGAGGGAT CCTCACCTCA CAGTGTCACT GACGAGGATG AGGGGTGGCT GTCTGATGGC 
ACTGGGGCTT TCTGCACCCC AGGGCTTCAG GCTAAAAAGG ATCTGGAGAG TCTGATCCAG 
AGAGTGTCTC AGCTGGAGGC CCAGCTCCCG AAAACTGGAG TGGAAGGGAA GCTAGCAGAG 
GAGCTGAGAT CAGCCTCATG GCCTGGGAAA TATGATTCCC TAATTCAGGA TCAGGCCCGA 
GAGCTGTCGT ACCTACGCCA GAAGATACGA GAAGGGAGAG GTATCTGTTA TCTTCTCACC 
CAGCATGCAA AAGATACAGT AAAATCTTTT GAGGACCTTC TTAGGAGCAA TGACATTGAC 
TACTACCTGG GCCAGAGCTT CCGTGAGCAA CTTGCCCAGG GAAGCCAGCT GACAGAGAGA 
CTCACCAGTA AACTCAGCAC CAAGGATCAT AAAAGTGAGA AAGATCAAGC TGGACTTGAA 
CCACTGGCCC TCAGGCTCAG CAGGGAGCTG CAGGAGAAGG AGAAAGTGAT TGAAGTCCTG 
CAGGCCAAGC TAGATGCTCG GTCTCTCACA CCCTCCAGCA GCCATGCATT GTCAGACTCC 
CACCGCTCTC CCAGCAGCTC GTCCTTCCTG TCTGATGAGC TGGAAGCCTG CTCTGACATG 
GATGTAGCCA GCGAGTACAC ACATTATGAA GAGAAGAAGG CTCCACCTGG TCACTCAGAT 
TCCATCCATC ATTTGAGTCA TTCTGCTGTA CTGTCTTCTA AACCATCAGC AACCAGTGTA 
CCTCGGGGGG TTAAGGCCGA ACCCAGCAGC AACCCCATCA GCTTGCCAGC TCCCCAGAAC 
CCCCCCCAAG AGGCCAGCCA GGCCCATCCA GGCCTTCATT TTCCCTCCAT ATCCACACTG 
GTTAGCCTTC CCCAGGCACC ACTGCCCTCA GCTCCACCCA GCTTCCTGCC TTTCAGCTCC 
ACTGGCCCTC CCCTCCTTGG CTGCTGTGAG ACACCTATGG TCTCCTTGGC AGAGGCTCAG 
CAGGAGCTGC AGATGCTGCA GAAACAATTG GGAGAAAGTG TCAGCACCGT CCATCCTGCT 
TCCCCAGCTG CATTGCCAAG CAACCATTTA GAAGCCAACT CTTCCCAATA TCTCAACCCT 
GCCCAGCCCC ACTCTCCTCC AACTGGCAGC ACAGAATTGG GCAGAATCCT GGAGCCTGGC 
TACCTGGGTA GCGGCAGCCA GTGGGATGTG ATGAGGCCTC AGAAGGGGAG TGTATCAGGC 
GACTTATCCT CAGGATCCTC TGTGTGTCAG CTTAACTCCA AACCCACAGG GGCTGACCTG 
CTAGAAGAAC ATCTTGGTGA AATCCGGAAC CTGCGCCAGC GCCTAGAGGA GTCCATCTGC 
ATCAATGACC GCCTGCGGGA ACAGCTGGAA CACCGGCTCA GCTGCACAGC ACGTGGCAAT 
GGATCCACCT CTAATTTCTA CAGTCCTGGC CTGGAACCCA CACCTCAGCT CTACAATGAG 
AACAGAGTCC TTAAGGAAGA AAACCGGAGC CTTCAGGCTC AGCTGAGTCA TGTTTCTAGA 
GAGCGCTCCC AGGAGACAGA ATGCCTGAGG GAGGCTCTAC TGTCCTCTCA ATCCCGCCTT 
CAAGAGCTGG AAATGGAGCT AGAGCACCAA AAGGAGGAAC GGCAGCAGCT TTTGGAAGAC 
TTGAAGGAGA AGCAGCAGGA GATCTTGCAC TTCCAGGAGG AGCGCCTGTC CCTCCAGGAG 
AAAGACTCTA GGCTGCAGCG TAAGCTGGCC CTACTGCAGC AACAGTGTGA AGAGAAACAG 
CAGCTCTTCC AGTCCCTCCA GTCAGAGCTA CAGATCTATG AGGCGCTTTA TGGCAGTTCC 
AAGAAGGGTC TGAAAGCTTC CACTTGGGAT GCCTATCACC AGGTCCCTTT GAGCAGTGAC 
TTGAGCCACC TGGTGGCAGA GATCCGAGCT TTGCGAGGGC AGCTGGAGCA AAGCATACAG 
GTGAATAACT GTCTGCGACT GCAGCTGGAG CAGCAGCTGG ATGGCGGAAG GAGCAAAACC 
AGCCTCAGTC CCTCTTCCAC CAGCCAGGAG GACCCTGGAG ACAAGCAAGA CTCAATTGCT 
TCCCCTCCAG TCCGAGATGT TGGCATGAAT TCTCCAGCTC TCATGTTCCC TAGCTCTTCT 
CCTGCTGCTC CAGGCTCAGA GACAGCTATT TTCAATAGGA TAAATGAGCT GAATTTGGAT 
GCTTTTCCAG TAATGAAGAA CTCTCCCAAG CTGGAGGGCG AAGCTATCGA TGGCTCCTTT 
GCCAATAAGC ACGGCCGCCA TGTGATTGGC CACATTGATG ACTATAGTGC CCTAAGACAG 
CAGATTGGTG AGGGCAAAGT GCTGGTCAAA AAGATAACAG CACTTGTGAG ATCAGCCTAC 
ACCCTCCCTG GCCTTGAAAC TCAGGCGACA GAGGTGCCAG GTGGCAAAGG CATCCATGAA 
CTTGGGAGCA GCACCCAAGC CCTGCAGCAC GTGCTGGAGG AGTCGGCCTC CCTCCTCAAT 
ATGTTCTGGC GAGCGGCCCT GCCAAGCTCC AGCCCTGCCC TGTCTGGCAA CATGGGACAG 
GCCATGAAAA AGGAACTTCT GGAACTGAGA ACCAAACTGA CCAAACAGGA GACCCTCCTT 
CAGTGCACAG CTGAGCGTCT AAAGACCACC CACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC ATTTGACCAG GACACATGAT GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
GCCAAGTCAG GAATCCGAGG AAAGCAAAAG AACACTTACA AGATGGCCTC TGTAAAGCCT 
TATTCCTGCC CCAGCAAAGG AGCCACGCAA GAAGAGGAGC CCCCAGAGGT CCTTCAAACA 
GCAGGAAGGA TGGGCCTGCC CCCCTTTTGT GCAGGTCCCA ATCTGCTGAG GAGGCTTGGA 
CCCCACTCCT CCGCACTGGC TGGAGGGTCC AGGAGGAGTC AGGCATGGCC CAGTGGACGA 
GGGGGAAAGG AAGGCAGCCT TTCCTGGGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630746.1
CDS	110..7942
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630689.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630689.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAATTGAA GAACTTCATC AAAGCCTGGC TTCCCAGGAG 
AAGCTTGTAG AACAGCTGTC TCAAGAGAAA CAACAACTGC TCCATCTGTT GGAGGAACCA 
ATGAGCATGG AAATACAGCC TGTGACTGAA GAGTTACTCA AGCAACAAAA GCTGGATTCA 
GATGAGACCA TTATAACTCA GCAGTCTGTA TCTGATTCCC ACTTGGCTGA ACTCCAGGAA 
AAAATCCAGC AAATGGAGGC CACCAACAAA ATTCTTCAAG AGAAACTGAA TGAAATGAGC 
TGTGAGCTAA AGTCTGCTCA GGAGTCATCT CAGAAGCAAG ATGGTACAAT TCAAAGCCTC 
AAGGAAACTC TGAAAAGTAG GGAAAATGAG ACCGAGGAGT TGTACCAGGT GATTGAAGGT 
CAAAATGACA CAATGGCAAA GCTTCGAGAA ATGCTACACC AAAGCCAGCT TGGACAACTT 
CATGTATTTG AGGGTCACAT AGGACAGGAG AGCTCACAGG GTACTTCCCC AGCTCAGCAA 
CAGATGGCTC TGCTTGATCT TCAGAGTGGT TTGTTCTACA GCCAGCTTGA GATTCAGAAA 
CTCCAGAGGT TGGTACGTCA AAAAGAACGC CAACTGGCTG ATGCCAAACG ATGTGTCCAA 
TTTGTAGAGG CTGCAGCCCA TGAAAGAGAA CAGCAGAAAG AGGCATCTTG GAAACATAAT 
CAGGAATTAC GGAAAGCCTT GCAGCAGCTC CAAGGAGAAT TAGAGAGTAA GAGCCAACAG 
CTTCGTACCC TGGAGACTGA AAAATGTAAT GAGATCAGAG CCCATGAGCA ACACATTCAA 
CACCTAAACC ATAGTCTGAG CCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTATCGAG ATAAGTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
CGGCAGCGAA TACAAGACCG AGATGTTGCT TTGGAGCGGG CTATAGATGA AAAGTTCTCC 
GCCCTGGAAG AGAAAGAAAA AGAACTGCGT CAATTTCATC TTGCTGTGCG AGAGCGTGAT 
CATGACTTAG AGAGATTGCG TGCTATTCTC TCCTCTAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGGCCAA AGGCCTAGAA GTAGAGCAGT TATCAGCTAC CTGTCAAAAC 
CTCCAGTGGC TGAAAGAAGA AAAGGAAACC AAATTTAGCC ATTGGCAGAA GGAACAAGAA 
AGTATCATTC AGCAGTTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTG 
AGTGCCACCT TGTTCTGTAA GCTTGGACCT GGGCAGAGTG AGGTAGCAGA AGAGCTGTGC 
CAGCGCCTAC AGCGAAAGGA AAGGATGCTA CAGGAACTGC TAACCGATCG AAACAAACAA 
GCAGTGGAAC ACGAAATAGA GATTCAAGGC CTGCTTCAGT CTATGAGCAC CAGGGAGCAG 
GAGAGCCAAG CCGCTGCAGG GAAGATGGTG CAAGCCCTTA TGGAAAGAAA TTCAGAACTA 
CAGGCCCTGC GCCAGTATTT AGGAGGTAAA GACTTCATGG TTTCCCAAGC ACTCATCTCT 
AACCAACCCA CAGAAGTCAC CTCCAACAGC CCTCGTCTTG GAGAGCAGAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAA AGATGATAGC ATTTCCCTGA CTGCCAAAGG GGATACCAGT 
ATACCCAGGT CCTCATTAGG AGACCAGGAT ACAGTTGCAG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAGCTTGA GCTCATGGCT AAGAAAGAAC GAGAAAGTCG GATGGAACTT 
TCTGCACTAC AGTCCATGGT GGCCGTGCAA GAGGAAGAGC TGCAGGTGCA GGCTGCTGAC 
ATGGAATCCC TGACCAGGAA TATACAGATT AAAGAAGACC TCATAAAGGA CCTGCAGATG 
CAACTGGTTG ATCCTGAAGA TATACCAGCT ATGGAACGCC TGACCCAAGA GGTCTTACTT 
CTTCGGGAAA AAGTTGCTTC AGTAGAATCA CAGGGTCAGG AAACGTTAGG AAATCGAAGA 
CAACAATTGC TGCTAATGCT CGAAGGGCTG GTGGATGAAC GGAGTCGGCT CAATGAAGCT 
TTACAAGCAG AGAGACAGCT GTACAGCAGC CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGTGGTCGG 
CTAGAAGAAG TTCTTGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCTGCC 
ATTGGAGGTG CAGCTGCAGG GGAAGATACA GAAGATGCTA GCACTGAGTT CACGGACAGC 
ATCGAGGAGG AGGCTGCACA CCATAATCAC CAGCAACTCA TCAAGGTGGC TTTGGAGAAA 
AGTCTGGCAA CTGTGGAGAC TCAGAACGTA TGTCTCCCCC CTCCTTCCTC AGCAGGAGGG 
GACAATAACA GGGGTCTTCA GGAGGAGATG CTCCACCTGA GGGCTGAGAT CCACCAGCAC 
TTAGAGGAGA AGAGGAGAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT CGAGGAAGAC 
AAGGAGAAGG GCGAGGTGAC AGTGGAAACT GTGGCAGCCA AAGGAGGTCT GAATGAAAAT 
AGCCTTCAGG CTGAGTTCAG AAAGCTCCAG GGAAAACTGA AGCATGCCCA CAACATTATC 
AACCTCCTCA AAGAGCAGCT GGTGCTGAGC AGCAAGGATG GGAATAGTAA ACTTAGTCCA 
GAGCTCCTAG TGCGCCTGGA CCGGGAGATC GACAGAATGA ACACAGAGCT GGTCTGTTCT 
CCGGAGAAGC CCCAGTGCCA GGAAGAAGAG CATATGACCA CAAGGCCCTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TACCCTCTCA GTGGATGGCC ACCAACTGGA TAACCGGCCC 
CAGGCCCAAG ACCCTGGGCC TCAGTCAGAA TTTAGCCTTC CTGGATCCAC CAAGCACCTG 
CGCTCCCAGC TGGCCCAATG CAGACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCGGAAGCCA CAGTCTTTGC ACAGGCCAAC CAACTGGAGA AATACCGAGT CATGCTTAGT 
GAATCCTTGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA TCTGGGCTAT 
GAAACCTGTG GCCGAAGTGA GAATGAGGCT GAGCGGGAGG AGAGCACCAG TCCCGAGTGT 
GAGGAGCCTG ACAGCCTCAG GGAAACAGTC CTGATGGTGG GGCACCTGGG CTCACTGCTG 
GCCAGCCCTC CAGGGAAGAA GCCCTTGGAA AGCCATCGGG GGAAGCAGGA GGAGTTCCAG 
GCATATGGAA AGTCAGAAGA CATCTCTGTC CTACATAAGG ACATCAGAGA TCTGAAGGCC 
CAGCTGCAGA ATGCCAACAA GATCATTCAG AATCTTAAGA GCCGGGTCCG GTCCCTCTCA 
GTTACAAGTG ATTATTCATC TAGTCTGGAG AGACCAAGAA AGCTGAAGGC CATTGGCACC 
CTTGAGGGAT CCTCACCTCA CAGTGTCACT GACGAGGATG AGGGGTGGCT GTCTGATGGC 
ACTGGGGCTT TCTGCACCCC AGGGCTTCAG GCTAAAAAGG ATCTGGAGAG TCTGATCCAG 
AGAGTGTCTC AGCTGGAGGC CCAGCTCCCG AAAACTGGAG TGGAAGGGAA GCTAGCAGAG 
GAGCTGAGAT CAGCCTCATG GCCTGGGAAA TATGATTCCC TAATTCAGGA TCAGGCCCGA 
GAGCTGTCGT ACCTACGCCA GAAGATACGA GAAGGGAGAG GTATCTGTTA TCTTCTCACC 
CAGCATGCAA AAGATACAGT AAAATCTTTT GAGGACCTTC TTAGGAGCAA TGACATTGAC 
TACTACCTGG GCCAGAGCTT CCGTGAGCAA CTTGCCCAGG GAAGCCAGCT GACAGAGAGA 
CTCACCAGTA AACTCAGCAC CAAGGATCAT AAAAGTGAGA AAGATCAAGC TGGACTTGAA 
CCACTGGCCC TCAGGCTCAG CAGGGAGCTG CAGGAGAAGG AGAAAGTGAT TGAAGTCCTG 
CAGGCCAAGC TAGATGCTCG GTCTCTCACA CCCTCCAGCA GCCATGCATT GTCAGACTCC 
CACCGCTCTC CCAGCAGCTC GTCCTTCCTG TCTGATGAGC TGGAAGCCTG CTCTGACATG 
GATGTAGCCA GCGAGTACAC ACATTATGAA GAGAAGAAGG CTCCACCTGG TCACTCAGAT 
TCCATCCATC ATTTGAGTCA TTCTGCTGTA CTGTCTTCTA AACCATCAGC AACCAGTGTA 
CCTCGGGGGG TTAAGGCCGA ACCCAGCAGC AACCCCATCA GCTTGCCAGC TCCCCAGAAC 
CCCCCCCAAG AGGCCAGCCA GGCCCATCCA GGCCTTCATT TTCCCTCCAT ATCCACACTG 
GTTAGCCTTC CCCAGGCACC ACTGCCCTCA GCTCCACCCA GCTTCCTGCC TTTCAGCTCC 
ACTGGCCCTC CCCTCCTTGG CTGCTGTGAG ACACCTATGG TCTCCTTGGC AGAGGCTCAG 
CAGGAGCTGC AGATGCTGCA GAAACAATTG GGAGAAAGTG TCAGCACCGT CCATCCTGCT 
TCCCCAGCTG CATTGCCAAG CAACCATTTA GAAGCCAACT CTTCCCAATA TCTCAACCCT 
GCCCAGCCCC ACTCTCCTCC AACTGGCAGC ACAGAATTGG GCAGAATCCT GGAGCCTGGC 
TACCTGGGTA GCGGCAGCCA GTGGGATGTG ATGAGGCCTC AGAAGGGGAG TGTATCAGGC 
GACTTATCCT CAGGATCCTC TGTGTGTCAG CTTAACTCCA AACCCACAGG GGCTGACCTG 
CTAGAAGAAC ATCTTGGTGA AATCCGGAAC CTGCGCCAGC GCCTAGAGGA GTCCATCTGC 
ATCAATGACC GCCTGCGGGA ACAGCTGGAA CACCGGCTCA GCTGCACAGC ACGTGGCAAT 
GGATCCACCT CTAATTTCTA CAGTCCTGGC CTGGAACCCA CACCTCAGCT CTACAATGAG 
AACAGAGTCC TTAAGGAAGA AAACCGGAGC CTTCAGGCTC AGCTGAGTCA TGTTTCTAGA 
GAGCGCTCCC AGGAGACAGA ATGCCTGAGG GAGGCTCTAC TGTCCTCTCA ATCCCGCCTT 
CAAGAGCTGG AAATGGAGCT AGAGCACCAA AAGGAGGAAC GGCAGCAGCT TTTGGAAGAC 
TTGAAGGAGA AGCAGCAGGA GATCTTGCAC TTCCAGGAGG AGCGCCTGTC CCTCCAGGAG 
AAAGACTCTA GGCTGCAGCG TAAGCTGGCC CTACTGCAGC AACAGTGTGA AGAGAAACAG 
CAGCTCTTCC AGTCCCTCCA GTCAGAGCTA CAGATCTATG AGGCGCTTTA TGGCAGTTCC 
AAGAAGGGTC TGAAAGCTTC CACTTGGGAT GCCTATCACC AGGTCCCTTT GAGCAGTGAC 
TTGAGCCACC TGGTGGCAGA GATCCGAGCT TTGCGAGGGC AGCTGGAGCA AAGCATACAG 
GTGAATAACT GTCTGCGACT GCAGCTGGAG CAGCAGCTGG ATGGCGGAAG GAGCAAAACC 
AGCCTCAGTC CCTCTTCCAC CAGCCAGGAG GACCCTGGAG ACAAGCAAGA CTCAATTGCT 
TCCCCTCCAG TCCGAGATGT TGGCATGAAT TCTCCAGCTC TCATGTTCCC TAGCTCTTCT 
CCTGCTGCTC CAGGCTCAGA GACAGCTATT TTCAATAGGA TAAATGAGCT GAATTTGGAT 
GCTTTTCCAG TAATGAAGAA CTCTCCCAAG CTGGAGGGCG AAGCTATCGA TGGCTCCTTT 
GCCAATAAGC ACGGCCGCCA TGTGATTGGC CACATTGATG ACTATAGTGC CCTAAGACAG 
CAGATTGGTG AGGGCAAAGT GCTGGTCAAA AAGATAACAG CACTTGTGAG ATCAGCCTAC 
ACCCTCCCTG GCCTTGAAAC TCAGGCGACA GAGGTGCCAG GTGGCAAAGG CATCCATGAA 
CTTGGGAGCA GCACCCAAGC CCTGCAGCAC GTGCTGGAGG AGTCGGCCTC CCTCCTCAAT 
ATGTTCTGGC GAGCGGCCCT GCCAAGCTCC AGCCCTGCCC TGTCTGGCAA CATGGGACAG 
GCCATGAAAA AGGAACTTCT GGAACTGAGA ACCAAACTGA CCAAACAGGA GACCCTCCTT 
CAGTGCACAG CTGAGCGTCT AAAGACCACC CACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC ATTTGACCAG GACACATGAT GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
GCCAAGTCAG GAATCCGAGG AAAGCAAAAG AACACTTACA AGATGGCCTC TGTAAAGCCT 
TATTCCTGCC CCAGCAAAGG AGCCACGCAA GAAGAGGAGC CCCCAGAGGT CCTTCAAACA 
GCAGGAAGGA TGGGCCTGCC CCCCTTTTGT GCAGGTCCCA ATCTGCTGAG GAGGCTTGGA 
CCCCACTCCT CCGCACTGGC TGGAGGGTCC AGGAGGAGTC AGGCATGGCC CAGTGGACGA 
GGGGGAAAGG AAGGCAGCCT TTCCTGGGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630691.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630748.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X7, mRNA.

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CloneID	OCe19358
Clone ID Related Accession (Same CDS sequence)	XM_005630691.2 , XM_005630691.3
Accession Version	XM_005630691.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGCC 
AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC ACTTACAAGA TGGCCTCTGT AAAGCCTTAT 
TCCTGCCCCA GCAAAGGAGC CACGCAAGAA GAGGAGCCCC CAGAGGTCCT TCAAACAGCA 
GGAAGGATGG GCCTGCCCCC CTTTTGTGCA GGTCCCAATC TGCTGAGGAG GCTTGGACCC 
CACTCCTCCG CACTGGCTGG AGGGTCCAGG AGGAGTCAGG CATGGCCCAG TGGACGAGGG 
GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630748.1
CDS	110..7759
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630691.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630691.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGCC 
AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC ACTTACAAGA TGGCCTCTGT AAAGCCTTAT 
TCCTGCCCCA GCAAAGGAGC CACGCAAGAA GAGGAGCCCC CAGAGGTCCT TCAAACAGCA 
GGAAGGATGG GCCTGCCCCC CTTTTGTGCA GGTCCCAATC TGCTGAGGAG GCTTGGACCC 
CACTCCTCCG CACTGGCTGG AGGGTCCAGG AGGAGTCAGG CATGGCCCAG TGGACGAGGG 
GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_005630691.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005630748.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X7, mRNA.

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CloneID	OCe19358
Clone ID Related Accession (Same CDS sequence)	XM_005630691.2 , XM_005630691.3
Accession Version	XM_005630691.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGCC 
AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC ACTTACAAGA TGGCCTCTGT AAAGCCTTAT 
TCCTGCCCCA GCAAAGGAGC CACGCAAGAA GAGGAGCCCC CAGAGGTCCT TCAAACAGCA 
GGAAGGATGG GCCTGCCCCC CTTTTGTGCA GGTCCCAATC TGCTGAGGAG GCTTGGACCC 
CACTCCTCCG CACTGGCTGG AGGGTCCAGG AGGAGTCAGG CATGGCCCAG TGGACGAGGG 
GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630748.1
CDS	16..7665
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_005630691.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630691.3

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGCC 
AAGTCAGGAA TCCGAGGAAA GCAAAAGAAC ACTTACAAGA TGGCCTCTGT AAAGCCTTAT 
TCCTGCCCCA GCAAAGGAGC CACGCAAGAA GAGGAGCCCC CAGAGGTCCT TCAAACAGCA 
GGAAGGATGG GCCTGCCCCC CTTTTGTGCA GGTCCCAATC TGCTGAGGAG GCTTGGACCC 
CACTCCTCCG CACTGGCTGG AGGGTCCAGG AGGAGTCAGG CATGGCCCAG TGGACGAGGG 
GGAAAGGAAG GCAGCCTTTC CTGGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_022404897.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260605.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X11, mRNA.

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CloneID	OCe294491
Clone ID Related Accession (Same CDS sequence)	XM_022404897.1
Accession Version	XM_022404897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA CCTCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260605.1
CDS	16..7614
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQPL

Target ORF information:

RefSeq Version	XM_022404897.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404897.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA CCTCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_022404899.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260607.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X18, mRNA.

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CloneID	OCe294493
Clone ID Related Accession (Same CDS sequence)	XM_022404899.1
Accession Version	XM_022404899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGAG 
CCACGCAAGA AGAGGAGCCC CCAGAGGTCC TTCAAACAGC AGGAAGGATG GGCCTGCCCC 
CCTTTTGTGC AGGTCCCAAT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260607.1
CDS	16..7482
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_022404899.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404899.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGAG 
CCACGCAAGA AGAGGAGCCC CCAGAGGTCC TTCAAACAGC AGGAAGGATG GGCCTGCCCC 
CCTTTTGTGC AGGTCCCAAT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120591.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976066.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X1, mRNA.

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CloneID	OCe40877
Clone ID Related Accession (Same CDS sequence)	XM_014120591.1 , XM_014120591.2
Accession Version	XM_014120591.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGAGCCACG CAAGAAGAGG AGCCCCCAGA GGTCCTTCAA 
ACAGCAGGAA GGATGGGCCT GCCCCCCTTT TGTGCAGGTC CCAATCTGCT GAGGAGGCTT 
GGACCCCACT CCTCCGCACT GGCTGGAGGG TCCAGGAGGA GTCAGGCATG GCCCAGTGGA 
CGAGGGGGAA AGGAAGGCAG CCTTTCCTGG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976066.1
CDS	110..7945
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120591.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120591.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGAGCCACG CAAGAAGAGG AGCCCCCAGA GGTCCTTCAA 
ACAGCAGGAA GGATGGGCCT GCCCCCCTTT TGTGCAGGTC CCAATCTGCT GAGGAGGCTT 
GGACCCCACT CCTCCGCACT GGCTGGAGGG TCCAGGAGGA GTCAGGCATG GCCCAGTGGA 
CGAGGGGGAA AGGAAGGCAG CCTTTCCTGG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120591.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976066.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X1, mRNA.

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CloneID	OCe40877
Clone ID Related Accession (Same CDS sequence)	XM_014120591.1 , XM_014120591.2
Accession Version	XM_014120591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGAGCCACG CAAGAAGAGG AGCCCCCAGA GGTCCTTCAA 
ACAGCAGGAA GGATGGGCCT GCCCCCCTTT TGTGCAGGTC CCAATCTGCT GAGGAGGCTT 
GGACCCCACT CCTCCGCACT GGCTGGAGGG TCCAGGAGGA GTCAGGCATG GCCCAGTGGA 
CGAGGGGGAA AGGAAGGCAG CCTTTCCTGG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976066.1
CDS	16..7851
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120591.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120591.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGAGCCACG CAAGAAGAGG AGCCCCCAGA GGTCCTTCAA 
ACAGCAGGAA GGATGGGCCT GCCCCCCTTT TGTGCAGGTC CCAATCTGCT GAGGAGGCTT 
GGACCCCACT CCTCCGCACT GGCTGGAGGG TCCAGGAGGA GTCAGGCATG GCCCAGTGGA 
CGAGGGGGAA AGGAAGGCAG CCTTTCCTGG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120592.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976067.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X3, mRNA.

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CloneID	OCe40878
Clone ID Related Accession (Same CDS sequence)	XM_014120592.1 , XM_014120592.2
Accession Version	XM_014120592.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGTGAAGTC CCTAAGGGCT CTGGCAAGCA CCCCAGTCTT 
GTGACCCTTG CCTTCCAGGA GCCACGCAAG AAGAGGAGCC CCCAGAGGTC CTTCAAACAG 
CAGGAAGGAT GGGCCTGCCC CCCTTTTGTG CAGGTCCCAA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976067.1
CDS	110..7897
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGEVPKGSGKHPSLVTLAFQEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_014120592.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120592.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGTGAAGTC CCTAAGGGCT CTGGCAAGCA CCCCAGTCTT 
GTGACCCTTG CCTTCCAGGA GCCACGCAAG AAGAGGAGCC CCCAGAGGTC CTTCAAACAG 
CAGGAAGGAT GGGCCTGCCC CCCTTTTGTG CAGGTCCCAA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120592.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976067.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X3, mRNA.

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CloneID	OCe40878
Clone ID Related Accession (Same CDS sequence)	XM_014120592.1 , XM_014120592.2
Accession Version	XM_014120592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGTGAAGTC CCTAAGGGCT CTGGCAAGCA CCCCAGTCTT 
GTGACCCTTG CCTTCCAGGA GCCACGCAAG AAGAGGAGCC CCCAGAGGTC CTTCAAACAG 
CAGGAAGGAT GGGCCTGCCC CCCTTTTGTG CAGGTCCCAA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976067.1
CDS	16..7803
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGEVPKGSGKHPSLVTLAFQEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_014120592.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120592.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA AAGAACACTT ACAAGATGGC CTCTGTAAAG 
CCTTATTCCT GCCCCAGCAA AGGTGAAGTC CCTAAGGGCT CTGGCAAGCA CCCCAGTCTT 
GTGACCCTTG CCTTCCAGGA GCCACGCAAG AAGAGGAGCC CCCAGAGGTC CTTCAAACAG 
CAGGAAGGAT GGGCCTGCCC CCCTTTTGTG CAGGTCCCAA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120593.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976068.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X5, mRNA.

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CloneID	OCe40879
Clone ID Related Accession (Same CDS sequence)	XM_014120593.1 , XM_014120593.2
Accession Version	XM_014120593.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GAGCCACGCA AGAAGAGGAG CCCCCAGAGG 
TCCTTCAAAC AGCAGGAAGG ATGGGCCTGC CCCCCTTTTG TGCAGGTCCC AATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976068.1
CDS	110..7789
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_014120593.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120593.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GAGCCACGCA AGAAGAGGAG CCCCCAGAGG 
TCCTTCAAAC AGCAGGAAGG ATGGGCCTGC CCCCCTTTTG TGCAGGTCCC AATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120593.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976068.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X5, mRNA.

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CloneID	OCe40879
Clone ID Related Accession (Same CDS sequence)	XM_014120593.1 , XM_014120593.2
Accession Version	XM_014120593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GAGCCACGCA AGAAGAGGAG CCCCCAGAGG 
TCCTTCAAAC AGCAGGAAGG ATGGGCCTGC CCCCCTTTTG TGCAGGTCCC AATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976068.1
CDS	16..7695
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_014120593.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120593.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GAGCCACGCA AGAAGAGGAG CCCCCAGAGG 
TCCTTCAAAC AGCAGGAAGG ATGGGCCTGC CCCCCTTTTG TGCAGGTCCC AATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120594.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976069.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X6, mRNA.

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CloneID	OCe40880
Clone ID Related Accession (Same CDS sequence)	XM_014120594.2 , XM_014120594.1
Accession Version	XM_014120594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGAGCCAC GCAAGAAGAG GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC 
TGCCCCCCTT TTGTGCAGGT CCCAATCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976069.1
CDS	16..7668
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_014120594.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120594.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGAGCCAC GCAAGAAGAG GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC 
TGCCCCCCTT TTGTGCAGGT CCCAATCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120594.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976069.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X6, mRNA.

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CloneID	OCe40880
Clone ID Related Accession (Same CDS sequence)	XM_014120594.2 , XM_014120594.1
Accession Version	XM_014120594.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGAGCCAC GCAAGAAGAG GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC 
TGCCCCCCTT TTGTGCAGGT CCCAATCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976069.1
CDS	110..7762
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEEPRKKRSPQRSFKQQEGWACPPFVQVPIC

Target ORF information:

RefSeq Version	XM_014120594.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120594.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGAGCCAC GCAAGAAGAG GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC 
TGCCCCCCTT TTGTGCAGGT CCCAATCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120595.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976070.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X8, mRNA.

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CloneID	OCe40881
Clone ID Related Accession (Same CDS sequence)	XM_014120595.1 , XM_014120595.2
Accession Version	XM_014120595.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GTGAAGTCCC TAAGGGCTCT GGCAAGCACC 
CCAGTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976070.1
CDS	110..7741
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120595.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120595.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GTGAAGTCCC TAAGGGCTCT GGCAAGCACC 
CCAGTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120595.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976070.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X8, mRNA.

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CloneID	OCe40881
Clone ID Related Accession (Same CDS sequence)	XM_014120595.1 , XM_014120595.2
Accession Version	XM_014120595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GTGAAGTCCC TAAGGGCTCT GGCAAGCACC 
CCAGTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976070.1
CDS	16..7647
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120595.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120595.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA GTGAAGTCCC TAAGGGCTCT GGCAAGCACC 
CCAGTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120596.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976071.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X9, mRNA.

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CloneID	OCe40882
Clone ID Related Accession (Same CDS sequence)	XM_014120596.1 , XM_014120596.2
Accession Version	XM_014120596.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA ACTCCTTTCT TTCCTGAGCC TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976071.1
CDS	110..7726
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQTPFFPEPL

Target ORF information:

RefSeq Version	XM_014120596.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120596.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA ACTCCTTTCT TTCCTGAGCC TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120596.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976071.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X9, mRNA.

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CloneID	OCe40882
Clone ID Related Accession (Same CDS sequence)	XM_014120596.1 , XM_014120596.2
Accession Version	XM_014120596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA ACTCCTTTCT TTCCTGAGCC TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976071.1
CDS	16..7632
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQTPFFPEPL

Target ORF information:

RefSeq Version	XM_014120596.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120596.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGCCAAGT CAGGAATCCG AGGAAAGCAA ACTCCTTTCT TTCCTGAGCC TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120597.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976072.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X10, mRNA.

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CloneID	OCe40883
Clone ID Related Accession (Same CDS sequence)	XM_014120597.2 , XM_014120597.1
Accession Version	XM_014120597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGTGAAGT CCCTAAGGGC TCTGGCAAGC ACCCCAGTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976072.1
CDS	16..7620
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120597.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120597.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGTGAAGT CCCTAAGGGC TCTGGCAAGC ACCCCAGTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120597.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976072.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X10, mRNA.

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CloneID	OCe40883
Clone ID Related Accession (Same CDS sequence)	XM_014120597.2 , XM_014120597.1
Accession Version	XM_014120597.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGTGAAGT CCCTAAGGGC TCTGGCAAGC ACCCCAGTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976072.1
CDS	110..7714
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGLHFPSISTLVSLPQAPLPSAPPSFLPFSSTGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120597.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120597.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGCCTTC ATTTTCCCTC CATATCCACA 
CTGGTTAGCC TTCCCCAGGC ACCACTGCCC TCAGCTCCAC CCAGCTTCCT GCCTTTCAGC 
TCCACTGGCC CTCCCCTCCT TGGCTGCTGT GAGACACCTA TGGTCTCCTT GGCAGAGGCT 
CAGCAGGAGC TGCAGATGCT GCAGAAACAA TTGGGAGAAA GTGTCAGCAC CGTCCATCCT 
GCTTCCCCAG CTGCATTGCC AAGCAACCAT TTAGAAGCCA ACTCTTCCCA ATATCTCAAC 
CCTGCCCAGC CCCACTCTCC TCCAACTGGC AGCACAGAAT TGGGCAGAAT CCTGGAGCCT 
GGCTACCTGG GTAGCGGCAG CCAGTGGGAT GTGATGAGGC CTCAGAAGGG GAGTGTATCA 
GGCGACTTAT CCTCAGGATC CTCTGTGTGT CAGCTTAACT CCAAACCCAC AGGGGCTGAC 
CTGCTAGAAG AACATCTTGG TGAAATCCGG AACCTGCGCC AGCGCCTAGA GGAGTCCATC 
TGCATCAATG ACCGCCTGCG GGAACAGCTG GAACACCGGC TCAGCTGCAC AGCACGTGGC 
AATGGATCCA CCTCTAATTT CTACAGTCCT GGCCTGGAAC CCACACCTCA GCTCTACAAT 
GAGAACAGAG TCCTTAAGGA AGAAAACCGG AGCCTTCAGG CTCAGCTGAG TCATGTTTCT 
AGAGAGCGCT CCCAGGAGAC AGAATGCCTG AGGGAGGCTC TACTGTCCTC TCAATCCCGC 
CTTCAAGAGC TGGAAATGGA GCTAGAGCAC CAAAAGGAGG AACGGCAGCA GCTTTTGGAA 
GACTTGAAGG AGAAGCAGCA GGAGATCTTG CACTTCCAGG AGGAGCGCCT GTCCCTCCAG 
GAGAAAGACT CTAGGCTGCA GCGTAAGCTG GCCCTACTGC AGCAACAGTG TGAAGAGAAA 
CAGCAGCTCT TCCAGTCCCT CCAGTCAGAG CTACAGATCT ATGAGGCGCT TTATGGCAGT 
TCCAAGAAGG GTCTGAAAGC TTCCACTTGG GATGCCTATC ACCAGGTCCC TTTGAGCAGT 
GACTTGAGCC ACCTGGTGGC AGAGATCCGA GCTTTGCGAG GGCAGCTGGA GCAAAGCATA 
CAGGTGAATA ACTGTCTGCG ACTGCAGCTG GAGCAGCAGC TGGATGGCGG AAGGAGCAAA 
ACCAGCCTCA GTCCCTCTTC CACCAGCCAG GAGGACCCTG GAGACAAGCA AGACTCAATT 
GCTTCCCCTC CAGTCCGAGA TGTTGGCATG AATTCTCCAG CTCTCATGTT CCCTAGCTCT 
TCTCCTGCTG CTCCAGGCTC AGAGACAGCT ATTTTCAATA GGATAAATGA GCTGAATTTG 
GATGCTTTTC CAGTAATGAA GAACTCTCCC AAGCTGGAGG GCGAAGCTAT CGATGGCTCC 
TTTGCCAATA AGCACGGCCG CCATGTGATT GGCCACATTG ATGACTATAG TGCCCTAAGA 
CAGCAGATTG GTGAGGGCAA AGTGCTGGTC AAAAAGATAA CAGCACTTGT GAGATCAGCC 
TACACCCTCC CTGGCCTTGA AACTCAGGCG ACAGAGGTGC CAGGTGGCAA AGGCATCCAT 
GAACTTGGGA GCAGCACCCA AGCCCTGCAG CACGTGCTGG AGGAGTCGGC CTCCCTCCTC 
AATATGTTCT GGCGAGCGGC CCTGCCAAGC TCCAGCCCTG CCCTGTCTGG CAACATGGGA 
CAGGCCATGA AAAAGGAACT TCTGGAACTG AGAACCAAAC TGACCAAACA GGAGACCCTC 
CTTCAGTGCA CAGCTGAGCG TCTAAAGACC ACCCACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCATTTGAC CAGGACACAT GATGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGGTGAAGT CCCTAAGGGC TCTGGCAAGC ACCCCAGTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120598.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976073.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X12, mRNA.

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CloneID	OCe40884
Clone ID Related Accession (Same CDS sequence)	XM_014120598.1 , XM_014120598.2
Accession Version	XM_014120598.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976073.1
CDS	111..7607
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120598.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120598.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120598.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976073.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X12, mRNA.

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CloneID	OCe40884
Clone ID Related Accession (Same CDS sequence)	XM_014120598.1 , XM_014120598.2
Accession Version	XM_014120598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976073.1
CDS	16..7512
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEAKSGIRGKQKNTYKMASVKPYSCPSKGATQEEEPPEVLQTAGRMGLPPFCAGPNLLRRLGPHSSALAGGSRRSQAWPSGRGGKEGSLSWG

Target ORF information:

RefSeq Version	XM_014120598.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120598.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GGTGTCAGCA CCGTCCATCC TGCTTCCCCA GCTGCATTGC CAAGCAACCA TTTAGAAGCC 
AACTCTTCCC AATATCTCAA CCCTGCCCAG CCCCACTCTC CTCCAACTGG CAGCACAGAA 
TTGGGCAGAA TCCTGGAGCC TGGCTACCTG GGTAGCGGCA GCCAGTGGGA TGTGATGAGG 
CCTCAGAAGG GGAGTGTATC AGGCGACTTA TCCTCAGGAT CCTCTGTGTG TCAGCTTAAC 
TCCAAACCCA CAGGGGCTGA CCTGCTAGAA GAACATCTTG GTGAAATCCG GAACCTGCGC 
CAGCGCCTAG AGGAGTCCAT CTGCATCAAT GACCGCCTGC GGGAACAGCT GGAACACCGG 
CTCAGCTGCA CAGCACGTGG CAATGGATCC ACCTCTAATT TCTACAGTCC TGGCCTGGAA 
CCCACACCTC AGCTCTACAA TGAGAACAGA GTCCTTAAGG AAGAAAACCG GAGCCTTCAG 
GCTCAGCTGA GTCATGTTTC TAGAGAGCGC TCCCAGGAGA CAGAATGCCT GAGGGAGGCT 
CTACTGTCCT CTCAATCCCG CCTTCAAGAG CTGGAAATGG AGCTAGAGCA CCAAAAGGAG 
GAACGGCAGC AGCTTTTGGA AGACTTGAAG GAGAAGCAGC AGGAGATCTT GCACTTCCAG 
GAGGAGCGCC TGTCCCTCCA GGAGAAAGAC TCTAGGCTGC AGCGTAAGCT GGCCCTACTG 
CAGCAACAGT GTGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCGC TTTATGGCAG TTCCAAGAAG GGTCTGAAAG CTTCCACTTG GGATGCCTAT 
CACCAGGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAAAGCAT ACAGGTGAAT AACTGTCTGC GACTGCAGCT GGAGCAGCAG 
CTGGATGGCG GAAGGAGCAA AACCAGCCTC AGTCCCTCTT CCACCAGCCA GGAGGACCCT 
GGAGACAAGC AAGACTCAAT TGCTTCCCCT CCAGTCCGAG ATGTTGGCAT GAATTCTCCA 
GCTCTCATGT TCCCTAGCTC TTCTCCTGCT GCTCCAGGCT CAGAGACAGC TATTTTCAAT 
AGGATAAATG AGCTGAATTT GGATGCTTTT CCAGTAATGA AGAACTCTCC CAAGCTGGAG 
GGCGAAGCTA TCGATGGCTC CTTTGCCAAT AAGCACGGCC GCCATGTGAT TGGCCACATT 
GATGACTATA GTGCCCTAAG ACAGCAGATT GGTGAGGGCA AAGTGCTGGT CAAAAAGATA 
ACAGCACTTG TGAGATCAGC CTACACCCTC CCTGGCCTTG AAACTCAGGC GACAGAGGTG 
CCAGGTGGCA AAGGCATCCA TGAACTTGGG AGCAGCACCC AAGCCCTGCA GCACGTGCTG 
GAGGAGTCGG CCTCCCTCCT CAATATGTTC TGGCGAGCGG CCCTGCCAAG CTCCAGCCCT 
GCCCTGTCTG GCAACATGGG ACAGGCCATG AAAAAGGAAC TTCTGGAACT GAGAACCAAA 
CTGACCAAAC AGGAGACCCT CCTTCAGTGC ACAGCTGAGC GTCTAAAGAC CACCCACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCATTTGA CCAGGACACA TGATGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGCCAAG TCAGGAATCC GAGGAAAGCA AAAGAACACT 
TACAAGATGG CCTCTGTAAA GCCTTATTCC TGCCCCAGCA AAGGAGCCAC GCAAGAAGAG 
GAGCCCCCAG AGGTCCTTCA AACAGCAGGA AGGATGGGCC TGCCCCCCTT TTGTGCAGGT 
CCCAATCTGC TGAGGAGGCT TGGACCCCAC TCCTCCGCAC TGGCTGGAGG GTCCAGGAGG 
AGTCAGGCAT GGCCCAGTGG ACGAGGGGGA AAGGAAGGCA GCCTTTCCTG GGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120602.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976077.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X18, mRNA.

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CloneID	OCe40888
Clone ID Related Accession (Same CDS sequence)	XM_014120602.1 , XM_014120602.2
Accession Version	XM_014120602.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myomegalin isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGTG 
AAGTCCCTAA GGGCTCTGGC AAGCACCCCA GTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976077.1
CDS	110..7528
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120602.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120602.1

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGTG 
AAGTCCCTAA GGGCTCTGGC AAGCACCCCA GTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100683419 ( XM_014120602.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976077.1 Canis lupus familiaris myomegalin (LOC100683419), transcript variant X19, mRNA.

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CloneID	OCe40888
Clone ID Related Accession (Same CDS sequence)	XM_014120602.1 , XM_014120602.2
Accession Version	XM_014120602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myomegalin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGTG 
AAGTCCCTAA GGGCTCTGGC AAGCACCCCA GTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976077.1
CDS	16..7434
Translation	MRGRGGCGGVCARGPEAVWAEQVAEAAAEEAWPLQVSATITSGGCSRAAREDRRKTEARRTAVGPGKSMKRADGGSCCRRRCPAHLAPYRSGDAARAPQPSLQSPGRERRHPEAAGSWAAAAEEEEAAAAAAPWMRDYFAEDEGEMVPRTSHAAAFLSDTKDRGPPVQSQIWRSGEKASFGQTPSLRAFEKPPQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLDKTWADVENLNSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVANDREEVPMDQVNPDQYTEALAQRDKRIEELHQSLASQEKLVEQLSQEKQQLLHLLEEPMSMEIQPVTEELLKQQKLDSDETIITQQSVSDSHLAELQEKIQQMEATNKILQEKLNEMSCELKSAQESSQKQDGTIQSLKETLKSRENETEELYQVIEGQNDTMAKLREMLHQSQLGQLHVFEGHIGQESSQGTSPAQQQMALLDLQSGLFYSQLEIQKLQRLVRQKERQLADAKRCVQFVEAAAHEREQQKEASWKHNQELRKALQQLQGELESKSQQLRTLETEKCNEIRAHEQHIQHLNHSLSHKEQLLQEFRELLQYRDKSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSALEEKEKELRQFHLAVRERDHDLERLRAILSSNEATMQSMESLLRAKGLEVEQLSATCQNLQWLKEEKETKFSHWQKEQESIIQQLQTSLHDRNKEVEDLSATLFCKLGPGQSEVAEELCQRLQRKERMLQELLTDRNKQAVEHEIEIQGLLQSMSTREQESQAAAGKMVQALMERNSELQALRQYLGGKDFMVSQALISNQPTEVTSNSPRLGEQTDQGSVHIPSKDDSISLTAKGDTSIPRSSLGDQDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETLGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRGRLEEVLGRSLERLSRLETLAAIGGAAAGEDTEDASTEFTDSIEEEAAHHNHQQLIKVALEKSLATVETQNVCLPPPSSAGGDNNRGLQEEMLHLRAEIHQHLEEKRRAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEVTVETVAAKGGLNENSLQAEFRKLQGKLKHAHNIINLLKEQLVLSSKDGNSKLSPELLVRLDREIDRMNTELVCSPEKPQCQEEEHMTTRPCPRPQSLDLGATLSVDGHQLDNRPQAQDPGPQSEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRVMLSESLVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEPDSLRETVLMVGHLGSLLASPPGKKPLESHRGKQEEFQAYGKSEDISVLHKDIRDLKAQLQNANKIIQNLKSRVRSLSVTSDYSSSLERPRKLKAIGTLEGSSPHSVTDEDEGWLSDGTGAFCTPGLQAKKDLESLIQRVSQLEAQLPKTGVEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSSSFLSDELEACSDMDVASEYTHYEEKKAPPGHSDSIHHLSHSAVLSSKPSATSVPRGVKAEPSSNPISLPAPQNPPQEASQAHPGVSTVHPASPAALPSNHLEANSSQYLNPAQPHSPPTGSTELGRILEPGYLGSGSQWDVMRPQKGSVSGDLSSGSSVCQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLSCTARGNGSTSNFYSPGLEPTPQLYNENRVLKEENRSLQAQLSHVSRERSQETECLREALLSSQSRLQELEMELEHQKEERQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGSSKKGLKASTWDAYHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGRSKTSLSPSSTSQEDPGDKQDSIASPPVRDVGMNSPALMFPSSSPAAPGSETAIFNRINELNLDAFPVMKNSPKLEGEAIDGSFANKHGRHVIGHIDDYSALRQQIGEGKVLVKKITALVRSAYTLPGLETQATEVPGGKGIHELGSSTQALQHVLEESASLLNMFWRAALPSSSPALSGNMGQAMKKELLELRTKLTKQETLLQCTAERLKTTHQQKESMEQFIFSHLTRTHDVLKKARTNLEVKSLRALASTPVL

Target ORF information:

RefSeq Version	XM_014120602.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myomegalin (LOC100683419), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120602.2

ORF Insert Sequence:

ATGAGAGGGC GAGGAGGCTG CGGCGGTGTT TGTGCGCGAG GCCCCGAAGC AGTCTGGGCG 
GAGCAGGTGG CAGAGGCAGC CGCGGAAGAG GCCTGGCCCT TGCAGGTTTC CGCCACCATC 
ACGTCTGGAG GATGCTCCCG GGCGGCGCGG GAGGACCGTA GGAAGACCGA GGCCAGGAGG 
ACCGCGGTCG GGCCCGGGAA ATCCATGAAG AGGGCGGACG GTGGGTCCTG CTGTCGCCGC 
CGCTGCCCCG CGCACCTCGC GCCTTACCGG TCCGGGGACG CGGCGCGAGC CCCGCAGCCC 
TCTCTGCAGT CTCCGGGCCG GGAGCGACGG CATCCCGAGG CGGCCGGCAG CTGGGCGGCG 
GCGGCCGAGG AGGAAGAGGC AGCGGCGGCC GCCGCACCCT GGATGAGAGA TTACTTTGCA 
GAGGATGAAG GGGAGATGGT ACCCAGAACA AGTCATGCAG CAGCTTTTCT TAGTGACACT 
AAAGATCGAG GCCCTCCAGT GCAATCACAG ATCTGGCGAA GTGGTGAAAA GGCCTCCTTT 
GGACAGACGC CTTCCTTGAG AGCATTTGAA AAACCCCCTC AGGTGCAGAC CCAGGCTCTT 
AGAGACTTTG AGAAGCATCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTATTTCC TGGAGGAACG CATGCAGCAG AAGTATGAGG CCAGCCGGGA GGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGGGAACT CCAGGACAAG 
AAACAGCATC TAGATAAAAC GTGGGCTGAT GTGGAGAATC TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GTCAGTTTGA AGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG CAAAGAATGA GGCTGCACGG 
ATGGCAGCTC TGGTGGAAGC TGAGAAAGAA TGTAACCTGG AGCTATCAGA GAAACTGAAG 
GGAGTTGCCA ATGACAGGGA AGAGGTACCA ATGGACCAGG TCAATCCTGA CCAATACACT 
GAGGCCCTGG CCCAGCGGGA CAAAAGAATT GAAGAACTTC ATCAAAGCCT GGCTTCCCAG 
GAGAAGCTTG TAGAACAGCT GTCTCAAGAG AAACAACAAC TGCTCCATCT GTTGGAGGAA 
CCAATGAGCA TGGAAATACA GCCTGTGACT GAAGAGTTAC TCAAGCAACA AAAGCTGGAT 
TCAGATGAGA CCATTATAAC TCAGCAGTCT GTATCTGATT CCCACTTGGC TGAACTCCAG 
GAAAAAATCC AGCAAATGGA GGCCACCAAC AAAATTCTTC AAGAGAAACT GAATGAAATG 
AGCTGTGAGC TAAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGGTAC AATTCAAAGC 
CTCAAGGAAA CTCTGAAAAG TAGGGAAAAT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAATG ACACAATGGC AAAGCTTCGA GAAATGCTAC ACCAAAGCCA GCTTGGACAA 
CTTCATGTAT TTGAGGGTCA CATAGGACAG GAGAGCTCAC AGGGTACTTC CCCAGCTCAG 
CAACAGATGG CTCTGCTTGA TCTTCAGAGT GGTTTGTTCT ACAGCCAGCT TGAGATTCAG 
AAACTCCAGA GGTTGGTACG TCAAAAAGAA CGCCAACTGG CTGATGCCAA ACGATGTGTC 
CAATTTGTAG AGGCTGCAGC CCATGAAAGA GAACAGCAGA AAGAGGCATC TTGGAAACAT 
AATCAGGAAT TACGGAAAGC CTTGCAGCAG CTCCAAGGAG AATTAGAGAG TAAGAGCCAA 
CAGCTTCGTA CCCTGGAGAC TGAAAAATGT AATGAGATCA GAGCCCATGA GCAACACATT 
CAACACCTAA ACCATAGTCT GAGCCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTATC GAGATAAGTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTCGGCAGC GAATACAAGA CCGAGATGTT GCTTTGGAGC GGGCTATAGA TGAAAAGTTC 
TCCGCCCTGG AAGAGAAAGA AAAAGAACTG CGTCAATTTC ATCTTGCTGT GCGAGAGCGT 
GATCATGACT TAGAGAGATT GCGTGCTATT CTCTCCTCTA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGGC CAAAGGCCTA GAAGTAGAGC AGTTATCAGC TACCTGTCAA 
AACCTCCAGT GGCTGAAAGA AGAAAAGGAA ACCAAATTTA GCCATTGGCA GAAGGAACAA 
GAAAGTATCA TTCAGCAGTT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTGAGTGCCA CCTTGTTCTG TAAGCTTGGA CCTGGGCAGA GTGAGGTAGC AGAAGAGCTG 
TGCCAGCGCC TACAGCGAAA GGAAAGGATG CTACAGGAAC TGCTAACCGA TCGAAACAAA 
CAAGCAGTGG AACACGAAAT AGAGATTCAA GGCCTGCTTC AGTCTATGAG CACCAGGGAG 
CAGGAGAGCC AAGCCGCTGC AGGGAAGATG GTGCAAGCCC TTATGGAAAG AAATTCAGAA 
CTACAGGCCC TGCGCCAGTA TTTAGGAGGT AAAGACTTCA TGGTTTCCCA AGCACTCATC 
TCTAACCAAC CCACAGAAGT CACCTCCAAC AGCCCTCGTC TTGGAGAGCA GACTGATCAA 
GGTTCAGTGC ATATACCCTC CAAAGATGAT AGCATTTCCC TGACTGCCAA AGGGGATACC 
AGTATACCCA GGTCCTCATT AGGAGACCAG GATACAGTTG CAGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAGCT TGAGCTCATG GCTAAGAAAG AACGAGAAAG TCGGATGGAA 
CTTTCTGCAC TACAGTCCAT GGTGGCCGTG CAAGAGGAAG AGCTGCAGGT GCAGGCTGCT 
GACATGGAAT CCCTGACCAG GAATATACAG ATTAAAGAAG ACCTCATAAA GGACCTGCAG 
ATGCAACTGG TTGATCCTGA AGATATACCA GCTATGGAAC GCCTGACCCA AGAGGTCTTA 
CTTCTTCGGG AAAAAGTTGC TTCAGTAGAA TCACAGGGTC AGGAAACGTT AGGAAATCGA 
AGACAACAAT TGCTGCTAAT GCTCGAAGGG CTGGTGGATG AACGGAGTCG GCTCAATGAA 
GCTTTACAAG CAGAGAGACA GCTGTACAGC AGCCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGTGGT 
CGGCTAGAAG AAGTTCTTGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCT 
GCCATTGGAG GTGCAGCTGC AGGGGAAGAT ACAGAAGATG CTAGCACTGA GTTCACGGAC 
AGCATCGAGG AGGAGGCTGC ACACCATAAT CACCAGCAAC TCATCAAGGT GGCTTTGGAG 
AAAAGTCTGG CAACTGTGGA GACTCAGAAC GTATGTCTCC CCCCTCCTTC CTCAGCAGGA 
GGGGACAATA ACAGGGGTCT TCAGGAGGAG ATGCTCCACC TGAGGGCTGA GATCCACCAG 
CACTTAGAGG AGAAGAGGAG AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATCGAGGAA 
GACAAGGAGA AGGGCGAGGT GACAGTGGAA ACTGTGGCAG CCAAAGGAGG TCTGAATGAA 
AATAGCCTTC AGGCTGAGTT CAGAAAGCTC CAGGGAAAAC TGAAGCATGC CCACAACATT 
ATCAACCTCC TCAAAGAGCA GCTGGTGCTG AGCAGCAAGG ATGGGAATAG TAAACTTAGT 
CCAGAGCTCC TAGTGCGCCT GGACCGGGAG ATCGACAGAA TGAACACAGA GCTGGTCTGT 
TCTCCGGAGA AGCCCCAGTG CCAGGAAGAA GAGCATATGA CCACAAGGCC CTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTACCCTC TCAGTGGATG GCCACCAACT GGATAACCGG 
CCCCAGGCCC AAGACCCTGG GCCTCAGTCA GAATTTAGCC TTCCTGGATC CACCAAGCAC 
CTGCGCTCCC AGCTGGCCCA ATGCAGACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCGGAAG CCACAGTCTT TGCACAGGCC AACCAACTGG AGAAATACCG AGTCATGCTT 
AGTGAATCCT TGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGATCTGGGC 
TATGAAACCT GTGGCCGAAG TGAGAATGAG GCTGAGCGGG AGGAGAGCAC CAGTCCCGAG 
TGTGAGGAGC CTGACAGCCT CAGGGAAACA GTCCTGATGG TGGGGCACCT GGGCTCACTG 
CTGGCCAGCC CTCCAGGGAA GAAGCCCTTG GAAAGCCATC GGGGGAAGCA GGAGGAGTTC 
CAGGCATATG GAAAGTCAGA AGACATCTCT GTCCTACATA AGGACATCAG AGATCTGAAG 
GCCCAGCTGC AGAATGCCAA CAAGATCATT CAGAATCTTA AGAGCCGGGT CCGGTCCCTC 
TCAGTTACAA GTGATTATTC ATCTAGTCTG GAGAGACCAA GAAAGCTGAA GGCCATTGGC 
ACCCTTGAGG GATCCTCACC TCACAGTGTC ACTGACGAGG ATGAGGGGTG GCTGTCTGAT 
GGCACTGGGG CTTTCTGCAC CCCAGGGCTT CAGGCTAAAA AGGATCTGGA GAGTCTGATC 
CAGAGAGTGT CTCAGCTGGA GGCCCAGCTC CCGAAAACTG GAGTGGAAGG GAAGCTAGCA 
GAGGAGCTGA GATCAGCCTC ATGGCCTGGG AAATATGATT CCCTAATTCA GGATCAGGCC 
CGAGAGCTGT CGTACCTACG CCAGAAGATA CGAGAAGGGA GAGGTATCTG TTATCTTCTC 
ACCCAGCATG CAAAAGATAC AGTAAAATCT TTTGAGGACC TTCTTAGGAG CAATGACATT 
GACTACTACC TGGGCCAGAG CTTCCGTGAG CAACTTGCCC AGGGAAGCCA GCTGACAGAG 
AGACTCACCA GTAAACTCAG CACCAAGGAT CATAAAAGTG AGAAAGATCA AGCTGGACTT 
GAACCACTGG CCCTCAGGCT CAGCAGGGAG CTGCAGGAGA AGGAGAAAGT GATTGAAGTC 
CTGCAGGCCA AGCTAGATGC TCGGTCTCTC ACACCCTCCA GCAGCCATGC ATTGTCAGAC 
TCCCACCGCT CTCCCAGCAG CTCGTCCTTC CTGTCTGATG AGCTGGAAGC CTGCTCTGAC 
ATGGATGTAG CCAGCGAGTA CACACATTAT GAAGAGAAGA AGGCTCCACC TGGTCACTCA 
GATTCCATCC ATCATTTGAG TCATTCTGCT GTACTGTCTT CTAAACCATC AGCAACCAGT 
GTACCTCGGG GGGTTAAGGC CGAACCCAGC AGCAACCCCA TCAGCTTGCC AGCTCCCCAG 
AACCCCCCCC AAGAGGCCAG CCAGGCCCAT CCAGGTGTCA GCACCGTCCA TCCTGCTTCC 
CCAGCTGCAT TGCCAAGCAA CCATTTAGAA GCCAACTCTT CCCAATATCT CAACCCTGCC 
CAGCCCCACT CTCCTCCAAC TGGCAGCACA GAATTGGGCA GAATCCTGGA GCCTGGCTAC 
CTGGGTAGCG GCAGCCAGTG GGATGTGATG AGGCCTCAGA AGGGGAGTGT ATCAGGCGAC 
TTATCCTCAG GATCCTCTGT GTGTCAGCTT AACTCCAAAC CCACAGGGGC TGACCTGCTA 
GAAGAACATC TTGGTGAAAT CCGGAACCTG CGCCAGCGCC TAGAGGAGTC CATCTGCATC 
AATGACCGCC TGCGGGAACA GCTGGAACAC CGGCTCAGCT GCACAGCACG TGGCAATGGA 
TCCACCTCTA ATTTCTACAG TCCTGGCCTG GAACCCACAC CTCAGCTCTA CAATGAGAAC 
AGAGTCCTTA AGGAAGAAAA CCGGAGCCTT CAGGCTCAGC TGAGTCATGT TTCTAGAGAG 
CGCTCCCAGG AGACAGAATG CCTGAGGGAG GCTCTACTGT CCTCTCAATC CCGCCTTCAA 
GAGCTGGAAA TGGAGCTAGA GCACCAAAAG GAGGAACGGC AGCAGCTTTT GGAAGACTTG 
AAGGAGAAGC AGCAGGAGAT CTTGCACTTC CAGGAGGAGC GCCTGTCCCT CCAGGAGAAA 
GACTCTAGGC TGCAGCGTAA GCTGGCCCTA CTGCAGCAAC AGTGTGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CGCTTTATGG CAGTTCCAAG 
AAGGGTCTGA AAGCTTCCAC TTGGGATGCC TATCACCAGG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAAAG CATACAGGTG 
AATAACTGTC TGCGACTGCA GCTGGAGCAG CAGCTGGATG GCGGAAGGAG CAAAACCAGC 
CTCAGTCCCT CTTCCACCAG CCAGGAGGAC CCTGGAGACA AGCAAGACTC AATTGCTTCC 
CCTCCAGTCC GAGATGTTGG CATGAATTCT CCAGCTCTCA TGTTCCCTAG CTCTTCTCCT 
GCTGCTCCAG GCTCAGAGAC AGCTATTTTC AATAGGATAA ATGAGCTGAA TTTGGATGCT 
TTTCCAGTAA TGAAGAACTC TCCCAAGCTG GAGGGCGAAG CTATCGATGG CTCCTTTGCC 
AATAAGCACG GCCGCCATGT GATTGGCCAC ATTGATGACT ATAGTGCCCT AAGACAGCAG 
ATTGGTGAGG GCAAAGTGCT GGTCAAAAAG ATAACAGCAC TTGTGAGATC AGCCTACACC 
CTCCCTGGCC TTGAAACTCA GGCGACAGAG GTGCCAGGTG GCAAAGGCAT CCATGAACTT 
GGGAGCAGCA CCCAAGCCCT GCAGCACGTG CTGGAGGAGT CGGCCTCCCT CCTCAATATG 
TTCTGGCGAG CGGCCCTGCC AAGCTCCAGC CCTGCCCTGT CTGGCAACAT GGGACAGGCC 
ATGAAAAAGG AACTTCTGGA ACTGAGAACC AAACTGACCA AACAGGAGAC CCTCCTTCAG 
TGCACAGCTG AGCGTCTAAA GACCACCCAC CAGCAGAAGG AGAGCATGGA GCAGTTCATC 
TTCAGCCATT TGACCAGGAC ACATGATGTT TTGAAGAAGG CAAGGACTAA TTTGGAGGTG 
AAGTCCCTAA GGGCTCTGGC AAGCACCCCA GTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mus musculus (house mouse)	Pde4dip	NP_001034465.2
Gallus gallus (chicken)	PDE4DIP	XP_423459.4
Danio rerio (zebrafish)	si:ch211-242b18.1	XP_005160855.1
Rattus norvegicus (Norway rat)	Pde4dip	NP_071777.1
	PDE4DIP	XP_002801779.1
Bos taurus (cattle)	PDE4DIP	XP_005204110.1
Homo sapiens (human)	PDE4DIP	NP_001185763.3
Canis lupus familiaris (dog)	PDE4DIP	XP_005630746.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC100683419
Entrez Gene ID	100683419
Full Name	myomegalin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

LOC100683419 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: