unc-54 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-54
Entrez Gene ID	259839
Full Name	Myosin-4
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_061195.5	NP_493596.1	Myosin-4
NM_061195.4	NP_493596.1	Myosin-4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH7B	XP_001160839.2
Gallus gallus (chicken)	MYH7B	NP_989918.1
Danio rerio (zebrafish)	myh7bb	XP_005162166.1
Caenorhabditis elegans (roundworm)	myo-3	NP_506065.2
Homo sapiens (human)	MYH7B	NP_065935.2
Bos taurus (cattle)	MYH7B	XP_003586892.2
Mus musculus (house mouse)	Myh7b	NP_001078847.1
Danio rerio (zebrafish)	myh7	XP_005162168.1
Caenorhabditis elegans (roundworm)	myo-2	NP_510092.2
Caenorhabditis elegans (roundworm)	myo-5	NP_505094.1
Rattus norvegicus (Norway rat)	Myh7b	NP_001101264.2
Caenorhabditis elegans (roundworm)	unc-54	NP_493596.1
Canis lupus familiaris (dog)	MYH7B	XP_534397.4
Caenorhabditis elegans (roundworm)	myo-1	NP_492053.1
Xenopus tropicalis (tropical clawed frog)	myh7b	XP_002932939.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In C. elegans, mutant alleles of the myosin heavy chain gene unc-54 corresponding to R1845W, E1883K and H1901L, were as effective as the wild-type myosin gene in rescuing the null mutant worms, indicating that they retain functionality.
Title: Myosin Storage Myopathy in C. elegans and Human Cultured Muscle Cells.

The following unc-54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335763	NM_061195.5 Latest version!	Caenorhabditis elegans Myosin-4 (unc-54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa335763	NM_061195.4	Caenorhabditis elegans Myosin-4 (unc-54), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-54 ( NM_061195.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493596.1 Caenorhabditis elegans Myosin-4 (unc-54), mRNA.

Price & Availability↑

CloneID	OCa335763
Clone ID Related Accession (Same CDS sequence)	NM_061195.5 , NM_061195.4
Accession Version	NM_061195.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Myosin-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_061195.4.

ATGGAGCACG AGAAGGACCC AGGATGGCAA TATCTCCGCC GTACCAGAGA GCAGGTTTTG 
GAGGATCAAT CTAAGCCTTA TGACTCCAAG AAGAACGTCT GGATCCCAGA TCCAGAAGAG 
GGATACCTTG CCGGAGAAAT CACCGCCACC AAGGGAGACC AGGTCACCAT CGTCACCGCT 
CGCGGAAATG AGGTCACCCT CAAGAAGGAG TTGGTTCAAG AAATGAATCC ACCAAAGTTC 
GAGAAGACCG AAGACATGTC CAACTTGTCT TTCCTCAACG ACGCCTCTGT CCTCCATAAC 
TTGCGTTCCC GTTACGCTGC TATGCTCATC TACACCTACT CCGGACTTTT CTGCGTTGTC 
ATCAACCCAT ACAAGCGTCT CCCAATCTAC ACCGACTCTT GCGCCCGTAT GTTCATGGGA 
AAGAGAAAGA CAGAAATGCC ACCACATTTG TTCGCTGTCT CTGACGAAGC CTACCGTAAC 
ATGCTCCAAG ACCACGAGAA CCAGTCTATG CTCATTACCG GAGAATCTGG AGCCGGAAAG 
ACTGAGAACA CCAAGAAGGT TATTTGCTAC TTCGCCGCTG TCGGTGCCTC CCAACAGGAA 
GGTGGAGCTG AAGTTGATCC AAATAAGAAG AAGGTCACCC TTGAGGACCA AATCGTCCAG 
ACCAATCCAG TACTTGAAGC CTTCGGTAAC GCCAAGACTG TCCGTAACAA CAACTCATCC 
CGTTTCGGAA AGTTCATCCG TATCCACTTC AACAAGCACG GACGCCTCGC ATCTTGCGAT 
ATCGAGCATT ACTTGCTCGA GAAATCTCGT GTCATCCGTC AAGCTCCAGG AGAGCGTTGC 
TACCACATCT TCTACCAAAT CTACTCTGAC TTCCGTCCAG AGCTCAAGAA GGAACTTCTT 
CTTGACTTGC CAATCAAGGA TTACTGGTTC GTCGCCCAGG CCGAGTTGAT CATTGACGGA 
ATCGATGACG TCGAAGAGTT CCAACTTACT GATGAAGCTT TCGACATCCT CAACTTCTCT 
GCCGTCGAGA AGCAGGATTG CTACCGCCTC ATGTCCGCTC ACATGCACAT GGGTAACATG 
AAGTTCAAGC AACGCCCACG TGAAGAGCAA GCTGAGCCAG ATGGTACCGA TGAGGCCGAG 
AAGGCATCCA ACATGTACGG AATCGGATGC GAGGAGTTCC TCAAGGCTTT GACCAAGCCA 
CGTGTCAAGG TCGGAACCGA ATGGGTATCC AAGGGACAGA ACTGCGAACA AGTCAATTGG 
GCTGTCGGAG CCATGGCCAA GGGTCTCTAC TCCCGTGTGT TCAACTGGCT TGTCAAGAAG 
TGTAACCTCA CTCTGGATCA AAAGGGTATT GATCGTGATT ATTTCATCGG TGTGCTCGAT 
ATCGCCGGTT TCGAAATCTT CGACTTCAAC TCCTTCGAGC AGTTGTGGAT TAACTTCGTA 
AACGAGAAGC TCCAACAATT CTTCAACCAT CACATGTTCG TCCTTGAACA GGAAGAATAC 
GCCCGTGAGG GTATTCAATG GGTCTTCATC GATTTCGGAC TTGATTTGCA AGCGTGTATC 
GAACTTATTG AGAAGCCACT CGGTATTATC TCCATGCTTG ATGAAGAGTG TATCGTACCA 
AAGGCTACTG ATTTGACCCT CGCTTCCAAG CTTGTCGATC AACATCTTGG CAAGCATCCA 
AACTTCGAGA AGCCAAAGCC ACCAAAGGGA AAGCAAGGAG AAGCTCACTT CGCCATGCGG 
CACTACGCCG GAACTGTGCG TTACAACTGC TTGAACTGGC TCGAGAAGAA CAAGGACCCC 
CTCAACGACA CCGTTGTCTC GGCAATGAAA CAATCCAAGG GAAACGATCT TCTCGTCGAG 
ATCTGGCAAG ACTACACCAC CCAGGAAGAG GCCGCCGCTA AGGCCAAGGA GGGAGGTGGC 
GGTGGAAAGA AGAAGGGAAA GTCCGGATCT TTCATGACCG TCTCTATGCT CTACAGAGAG 
TCTCTCAACA ACTTGATGAC TATGCTCAAC AAGACTCACC CACATTTCAT CCGTTGTATC 
ATCCCCAACG AGAAGAAGCA ATCTGGTATG ATCGATGCCG CTTTGGTTCT CAACCAGCTT 
ACCTGCAACG GAGTGTTGGA AGGAATCAGA ATTTGCAGAA AGGGATTCCC CAACAGAACC 
CTTCATCCAG ACTTCGTCCA ACGTTACGCC ATCCTTGCCG CCAAGGAGGC CAAGTCCGAT 
GACGACAAGA AGAAGTGCGC CGAGGCTATC ATGTCCAAGC TCGTCAACGA CGGATCCCTC 
AGCGAGGAGA TGTTCCGTAT CGGTCTCACC AAGGTCTTCT TCAAGGCTGG AGTTCTTGCT 
CATCTTGAAG ACATCCGTGA CGAGAAGCTC GCCACCATCC TCACCGGATT CCAATCCCAA 
ATCAGATGGC ATTTGGGTCT CAAGGACCGC AAGCGCCGTA TGGAACAACG TGCCGGACTT 
CTCATTGTTC AGCGCAACGT CCGTTCCTGG TGCACTCTCC GTACCTGGGA ATGGTTCAAG 
CTTTACGGAA AGGTCAAGCC AATGCTCAAG GCCGGAAAGG AAGCCGAGGA GCTCGAGAAG 
ATCAACGACA AGGTTAAGGC CCTCGAAGAC AGTCTCGCCA AGGAGGAGAA GCTTCGCAAG 
GAGCTCGAGG AGTCCTCTGC CAAGCTCGTC GAAGAGAAAA CTTCTCTCTT CACCAACTTG 
GAATCCACCA AGACTCAACT CTCCGATGCT GAGGAAAGAC TTGCCAAGCT TGAGGCTCAA 
CAAAAGGATG CCTCCAAGCA ACTCTCCGAG CTCAACGATC AACTCGCTGA CAACGAAGAC 
CGTACCGCTG ATGTTCAGCG CGCTAAGAAG AAGATCGAGG CCGAGGTCGA GGCTTTGAAG 
AAGCAGATCC AAGACTTGGA AATGTCTCTC CGCAAGGCTG AGTCTGAGAA GCAATCCAAG 
GATCACCAGA TCAGATCCCT TCAAGATGAG ATGCAACAGC AAGATGAAGC TATTGCCAAG 
CTCAACAAGG AGAAGAAGCA TCAAGAAGAA ATCAACCGCA AGCTGATGGA GGACCTCCAA 
TCCGAAGAGG ATAAGGGTAA CCATCAGAAC AAGGTCAAGG CCAAGCTTGA GCAGACTCTT 
GATGACCTCG AGGATTCCCT TGAGCGCGAG AAGAGAGCCC GCGCCGATCT TGACAAGCAG 
AAGAGAAAGG TCGAGGGAGA GCTCAAGATT GCTCAAGAGA ACATCGATGA GAGCGGACGC 
CAACGCCACG ATCTTGAGAA CAACTTGAAG AAGAAGGAAT CTGAGTTGCA CTCAGTTTCT 
TCCCGACTTG AGGACGAACA AGCTCTTGTC TCCAAGCTCC AACGCCAGAT CAAGGACGGA 
CAAAGCCGTA TCTCCGAGCT CGAAGAGGAA CTCGAGAATG AACGTCAATC CCGTTCCAAG 
GCTGACCGTG CCAAGAGCGA CCTCCAACGC GAGTTGGAAG AGCTCGGTGA GAAGCTTGAC 
GAGCAAGGTG GAGCTACTGC CGCCCAGGTT GAGGTCAACA AGAAGCGTGA GGCTGAACTT 
GCCAAGCTCC GCAGAGACTT GGAGGAGGCC AACATGAACC ACGAGAACCA ACTCGGTGGA 
CTTCGCAAGA AGCACACCGA CGCTGTCGCT GAGCTCACCG ACCAACTCGA TCAACTCAAC 
AAGGCCAAGG CTAAGGTCGA GAAGGACAAG GCTCAAGCTG TTCGTGACGC TGAGGACCTT 
GCTGCTCAAC TTGACCAAGA AACCTCTGGA AAGCTCAACA ACGAAAAGCT TGCCAAGCAG 
TTCGAACTTC AACTCACCGA GCTTCAATCT AAGGCTGATG AGCAATCTCG CCAACTCCAA 
GACTTCACTT CCCTTAAGGG ACGCCTTCAC TCTGAGAATG GAGACCTTGT CCGTCAACTC 
GAAGATGCCG AATCCCAAGT CAACCAACTC ACCAGACTGA AGTCCCAGCT TACTTCTCAA 
CTCGAGGAGG CTCGCCGCAC CGCTGACGAG GAAGCACGCG AACGTCAAAC CGTCGCCGCC 
CAGGCTAAGA ACTACCAACA CGAAGCCGAG CAACTCCAAG AGTCCCTTGA AGAGGAGATC 
GAAGGAAAGA ACGAGATCCT CAGACAACTC TCCAAGGCTA ACGCCGACAT CCAACAATGG 
AAGGCTCGCT TCGAGGGAGA AGGACTCCTC AAGGCCGACG AGCTCGAGGA TGCCAAGAGA 
CGCCAAGCCC AAAAGATCAA CGAGCTCCAA GAGGCTCTTG ATGCCGCCAA CTCTAAGAAC 
GCTTCTCTTG AGAAGACCAA GTCCAGACTT GTCGGAGACT TGGACGACGC TCAAGTTGAT 
GTCGAGAGAG CCAACGGTGT CGCCAGTGCT CTTGAGAAGA AGCAAAAGGG CTTCGACAAG 
ATCATCGACG AATGGAGAAA GAAGACCGAC GACTTGGCCG CTGAGCTTGA TGGAGCTCAA 
CGCGACCTTC GCAACACCTC CACTGATCTC TTCAAGGCCA AGAACGCCCA AGAGGAGCTC 
GCCGAGGTTG TTGAGGGACT CCGCCGTGAG AACAAGAGCT TGAGCCAAGA GATCAAGGAT 
CTTACCGATC AACTCGGAGA GGGAGGACGC TCTGTCCACG AAATGCAAAA GATCATCCGC 
CGTCTTGAGA TTGAGAAGGA AGAACTCCAA CACGCTTTGG ACGAGGCTGA GGCTGCCCTT 
GAAGCTGAAG AGAGCAAGGT TCTCCGCGCC CAGGTTGAAG TTTCCCAGAT CCGTTCCGAA 
ATCGAGAAAC GCATCCAGGA GAAGGAGGAA GAGTTCGAGA ACACGAGAAA GAACCACGCC 
CGCGCTCTTG AATCAATGCA AGCTTCCCTC GAGACCGAAG CTAAAGGAAA GGCCGAACTT 
CTCCGCATCA AGAAGAAGCT CGAGGGAGAT ATCAACGAGC TCGAGATCGC TTTGGACCAC 
GCCAACAAGG CTAACGCCGA TGCCCAGAAG AACTTGAAGA GATACCAAGA GCAAGTCCGC 
GAGTTGCAAT TGCAAGTCGA GGAGGAGCAA CGCAATGGAG CCGACACCCG TGAACAATTC 
TTCAACGCCG AGAAGCGCGC CACTCTTCTT CAATCCGAGA AGGAAGAACT TTTGGTTGCC 
AACGAGGCCG CCGAGAGAGC CCGCAAGCAA GCCGAGTATG AAGCCGCCGA TGCTCGTGAT 
CAAGCCAACG AAGCCAACGC TCAAGTCAGC AGCTTGACTT CTGCCAAGAG AAAGCTCGAG 
GGAGAAATCC AAGCCATTCA TGCCGACCTC GATGAGACCC TCAACGAGTA CAAGGCCGCT 
GAGGAGCGCT CCAAGAAGGC TATTGCTGAT GCCACCAGAC TCGCAGAGGA GCTCCGTCAA 
GAACAAGAAC ACTCTCAGCA CGTTGACCGT CTTCGCAAGG GACTTGAGCA ACAGCTCAAG 
GAGATCCAAG TCCGTCTTGA TGAGGCCGAG GCTGCTGCTC TTAAGGGAGG AAAGAAGGTT 
ATCGCCAAGC TCGAACAACG CGTGCGTGAG CTCGAATCTG AATTGGACGG AGAACAACGC 
CGCTTCCAGG ATGCCAACAA GAACCTCGGA CGCGCCGATA GACGTGTTCG TGAGCTTCAA 
TTCCAGGTTG ATGAGGACAA GAAGAACTTC GAACGTCTCC AAGATCTCAT TGACAAGCTC 
CAACAGAAGT TGAAGACCCA GAAGAAGCAG GTTGAGGAAG CTGAGGAACT CGCCAACTTG 
AACCTCCAGA AGTACAAGCA ACTCACCCAC CAACTTGAAG ATGCTGAGGA GCGCGCCGAT 
CAAGCCGAAA ACTCCCTCTC AAAGATGCGA TCAAAATCCC GTGCATCCGC CTCTGTTGCC 
CCAGGACTCC AGAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC 
TCTGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493596.1
CDS	33..5924
Translation	MEHEKDPGWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTARGNEVTLKKELVQEMNPPKFEKTEDMSNLSFLNDASVLHNLRSRYAAMLIYTYSGLFCVVINPYKRLPIYTDSCARMFMGKRKTEMPPHLFAVSDEAYRNMLQDHENQSMLITGESGAGKTENTKKVICYFAAVGASQQEGGAEVDPNKKKVTLEDQIVQTNPVLEAFGNAKTVRNNNSSRFGKFIRIHFNKHGRLASCDIEHYLLEKSRVIRQAPGERCYHIFYQIYSDFRPELKKELLLDLPIKDYWFVAQAELIIDGIDDVEEFQLTDEAFDILNFSAVEKQDCYRLMSAHMHMGNMKFKQRPREEQAEPDGTDEAEKASNMYGIGCEEFLKALTKPRVKVGTEWVSKGQNCEQVNWAVGAMAKGLYSRVFNWLVKKCNLTLDQKGIDRDYFIGVLDIAGFEIFDFNSFEQLWINFVNEKLQQFFNHHMFVLEQEEYAREGIQWVFIDFGLDLQACIELIEKPLGIISMLDEECIVPKATDLTLASKLVDQHLGKHPNFEKPKPPKGKQGEAHFAMRHYAGTVRYNCLNWLEKNKDPLNDTVVSAMKQSKGNDLLVEIWQDYTTQEEAAAKAKEGGGGGKKKGKSGSFMTVSMLYRESLNNLMTMLNKTHPHFIRCIIPNEKKQSGMIDAALVLNQLTCNGVLEGIRICRKGFPNRTLHPDFVQRYAILAAKEAKSDDDKKKCAEAIMSKLVNDGSLSEEMFRIGLTKVFFKAGVLAHLEDIRDEKLATILTGFQSQIRWHLGLKDRKRRMEQRAGLLIVQRNVRSWCTLRTWEWFKLYGKVKPMLKAGKEAEELEKINDKVKALEDSLAKEEKLRKELEESSAKLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKDASKQLSELNDQLADNEDRTADVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKDHQIRSLQDEMQQQDEAIAKLNKEKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQTLDDLEDSLEREKRARADLDKQKRKVEGELKIAQENIDESGRQRHDLENNLKKKESELHSVSSRLEDEQALVSKLQRQIKDGQSRISELEEELENERQSRSKADRAKSDLQRELEELGEKLDEQGGATAAQVEVNKKREAELAKLRRDLEEANMNHENQLGGLRKKHTDAVAELTDQLDQLNKAKAKVEKDKAQAVRDAEDLAAQLDQETSGKLNNEKLAKQFELQLTELQSKADEQSRQLQDFTSLKGRLHSENGDLVRQLEDAESQVNQLTRLKSQLTSQLEEARRTADEEARERQTVAAQAKNYQHEAEQLQESLEEEIEGKNEILRQLSKANADIQQWKARFEGEGLLKADELEDAKRRQAQKINELQEALDAANSKNASLEKTKSRLVGDLDDAQVDVERANGVASALEKKQKGFDKIIDEWRKKTDDLAAELDGAQRDLRNTSTDLFKAKNAQEELAEVVEGLRRENKSLSQEIKDLTDQLGEGGRSVHEMQKIIRRLEIEKEELQHALDEAEAALEAEESKVLRAQVEVSQIRSEIEKRIQEKEEEFENTRKNHARALESMQASLETEAKGKAELLRIKKKLEGDINELEIALDHANKANADAQKNLKRYQEQVRELQLQVEEEQRNGADTREQFFNAEKRATLLQSEKEELLVANEAAERARKQAEYEAADARDQANEANAQVSSLTSAKRKLEGEIQAIHADLDETLNEYKAAEERSKKAIADATRLAEELRQEQEHSQHVDRLRKGLEQQLKEIQVRLDEAEAAALKGGKKVIAKLEQRVRELESELDGEQRRFQDANKNLGRADRRVRELQFQVDEDKKNFERLQDLIDKLQQKLKTQKKQVEEAEELANLNLQKYKQLTHQLEDAEERADQAENSLSKMRSKSRASASVAPGLQSSASAAVIRSPSRARASDF

Target ORF information:

RefSeq Version	NM_061195.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Myosin-4 (unc-54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061195.5

ORF Insert Sequence:

ATGGAGCACG AGAAGGACCC AGGATGGCAA TATCTCCGCC GTACCAGAGA GCAGGTTTTG 
GAGGATCAAT CTAAGCCTTA TGACTCCAAG AAGAACGTCT GGATCCCAGA TCCAGAAGAG 
GGATACCTTG CCGGAGAAAT CACCGCCACC AAGGGAGACC AGGTCACCAT CGTCACCGCT 
CGCGGAAATG AGGTCACCCT CAAGAAGGAG TTGGTTCAAG AAATGAATCC ACCAAAGTTC 
GAGAAGACCG AAGACATGTC CAACTTGTCT TTCCTCAACG ACGCCTCTGT CCTCCATAAC 
TTGCGTTCCC GTTACGCTGC TATGCTCATC TACACCTACT CCGGACTTTT CTGCGTTGTC 
ATCAACCCAT ACAAGCGTCT CCCAATCTAC ACCGACTCTT GCGCCCGTAT GTTCATGGGA 
AAGAGAAAGA CAGAAATGCC ACCACATTTG TTCGCTGTCT CTGACGAAGC CTACCGTAAC 
ATGCTCCAAG ACCACGAGAA CCAGTCTATG CTCATTACCG GAGAATCTGG AGCCGGAAAG 
ACTGAGAACA CCAAGAAGGT TATTTGCTAC TTCGCCGCTG TCGGTGCCTC CCAACAGGAA 
GGTGGAGCTG AAGTTGATCC AAATAAGAAG AAGGTCACCC TTGAGGACCA AATCGTCCAG 
ACCAATCCAG TACTTGAAGC CTTCGGTAAC GCCAAGACTG TCCGTAACAA CAACTCATCC 
CGTTTCGGAA AGTTCATCCG TATCCACTTC AACAAGCACG GACGCCTCGC ATCTTGCGAT 
ATCGAGCATT ACTTGCTCGA GAAATCTCGT GTCATCCGTC AAGCTCCAGG AGAGCGTTGC 
TACCACATCT TCTACCAAAT CTACTCTGAC TTCCGTCCAG AGCTCAAGAA GGAACTTCTT 
CTTGACTTGC CAATCAAGGA TTACTGGTTC GTCGCCCAGG CCGAGTTGAT CATTGACGGA 
ATCGATGACG TCGAAGAGTT CCAACTTACT GATGAAGCTT TCGACATCCT CAACTTCTCT 
GCCGTCGAGA AGCAGGATTG CTACCGCCTC ATGTCCGCTC ACATGCACAT GGGTAACATG 
AAGTTCAAGC AACGCCCACG TGAAGAGCAA GCTGAGCCAG ATGGTACCGA TGAGGCCGAG 
AAGGCATCCA ACATGTACGG AATCGGATGC GAGGAGTTCC TCAAGGCTTT GACCAAGCCA 
CGTGTCAAGG TCGGAACCGA ATGGGTATCC AAGGGACAGA ACTGCGAACA AGTCAATTGG 
GCTGTCGGAG CCATGGCCAA GGGTCTCTAC TCCCGTGTGT TCAACTGGCT TGTCAAGAAG 
TGTAACCTCA CTCTGGATCA AAAGGGTATT GATCGTGATT ATTTCATCGG TGTGCTCGAT 
ATCGCCGGTT TCGAAATCTT CGACTTCAAC TCCTTCGAGC AGTTGTGGAT TAACTTCGTA 
AACGAGAAGC TCCAACAATT CTTCAACCAT CACATGTTCG TCCTTGAACA GGAAGAATAC 
GCCCGTGAGG GTATTCAATG GGTCTTCATC GATTTCGGAC TTGATTTGCA AGCGTGTATC 
GAACTTATTG AGAAGCCACT CGGTATTATC TCCATGCTTG ATGAAGAGTG TATCGTACCA 
AAGGCTACTG ATTTGACCCT CGCTTCCAAG CTTGTCGATC AACATCTTGG CAAGCATCCA 
AACTTCGAGA AGCCAAAGCC ACCAAAGGGA AAGCAAGGAG AAGCTCACTT CGCCATGCGG 
CACTACGCCG GAACTGTGCG TTACAACTGC TTGAACTGGC TCGAGAAGAA CAAGGACCCC 
CTCAACGACA CCGTTGTCTC GGCAATGAAA CAATCCAAGG GAAACGATCT TCTCGTCGAG 
ATCTGGCAAG ACTACACCAC CCAGGAAGAG GCCGCCGCTA AGGCCAAGGA GGGAGGTGGC 
GGTGGAAAGA AGAAGGGAAA GTCCGGATCT TTCATGACCG TCTCTATGCT CTACAGAGAG 
TCTCTCAACA ACTTGATGAC TATGCTCAAC AAGACTCACC CACATTTCAT CCGTTGTATC 
ATCCCCAACG AGAAGAAGCA ATCTGGTATG ATCGATGCCG CTTTGGTTCT CAACCAGCTT 
ACCTGCAACG GAGTGTTGGA AGGAATCAGA ATTTGCAGAA AGGGATTCCC CAACAGAACC 
CTTCATCCAG ACTTCGTCCA ACGTTACGCC ATCCTTGCCG CCAAGGAGGC CAAGTCCGAT 
GACGACAAGA AGAAGTGCGC CGAGGCTATC ATGTCCAAGC TCGTCAACGA CGGATCCCTC 
AGCGAGGAGA TGTTCCGTAT CGGTCTCACC AAGGTCTTCT TCAAGGCTGG AGTTCTTGCT 
CATCTTGAAG ACATCCGTGA CGAGAAGCTC GCCACCATCC TCACCGGATT CCAATCCCAA 
ATCAGATGGC ATTTGGGTCT CAAGGACCGC AAGCGCCGTA TGGAACAACG TGCCGGACTT 
CTCATTGTTC AGCGCAACGT CCGTTCCTGG TGCACTCTCC GTACCTGGGA ATGGTTCAAG 
CTTTACGGAA AGGTCAAGCC AATGCTCAAG GCCGGAAAGG AAGCCGAGGA GCTCGAGAAG 
ATCAACGACA AGGTTAAGGC CCTCGAAGAC AGTCTCGCCA AGGAGGAGAA GCTTCGCAAG 
GAGCTCGAGG AGTCCTCTGC CAAGCTCGTC GAAGAGAAAA CTTCTCTCTT CACCAACTTG 
GAATCCACCA AGACTCAACT CTCCGATGCT GAGGAAAGAC TTGCCAAGCT TGAGGCTCAA 
CAAAAGGATG CCTCCAAGCA ACTCTCCGAG CTCAACGATC AACTCGCTGA CAACGAAGAC 
CGTACCGCTG ATGTTCAGCG CGCTAAGAAG AAGATCGAGG CCGAGGTCGA GGCTTTGAAG 
AAGCAGATCC AAGACTTGGA AATGTCTCTC CGCAAGGCTG AGTCTGAGAA GCAATCCAAG 
GATCACCAGA TCAGATCCCT TCAAGATGAG ATGCAACAGC AAGATGAAGC TATTGCCAAG 
CTCAACAAGG AGAAGAAGCA TCAAGAAGAA ATCAACCGCA AGCTGATGGA GGACCTCCAA 
TCCGAAGAGG ATAAGGGTAA CCATCAGAAC AAGGTCAAGG CCAAGCTTGA GCAGACTCTT 
GATGACCTCG AGGATTCCCT TGAGCGCGAG AAGAGAGCCC GCGCCGATCT TGACAAGCAG 
AAGAGAAAGG TCGAGGGAGA GCTCAAGATT GCTCAAGAGA ACATCGATGA GAGCGGACGC 
CAACGCCACG ATCTTGAGAA CAACTTGAAG AAGAAGGAAT CTGAGTTGCA CTCAGTTTCT 
TCCCGACTTG AGGACGAACA AGCTCTTGTC TCCAAGCTCC AACGCCAGAT CAAGGACGGA 
CAAAGCCGTA TCTCCGAGCT CGAAGAGGAA CTCGAGAATG AACGTCAATC CCGTTCCAAG 
GCTGACCGTG CCAAGAGCGA CCTCCAACGC GAGTTGGAAG AGCTCGGTGA GAAGCTTGAC 
GAGCAAGGTG GAGCTACTGC CGCCCAGGTT GAGGTCAACA AGAAGCGTGA GGCTGAACTT 
GCCAAGCTCC GCAGAGACTT GGAGGAGGCC AACATGAACC ACGAGAACCA ACTCGGTGGA 
CTTCGCAAGA AGCACACCGA CGCTGTCGCT GAGCTCACCG ACCAACTCGA TCAACTCAAC 
AAGGCCAAGG CTAAGGTCGA GAAGGACAAG GCTCAAGCTG TTCGTGACGC TGAGGACCTT 
GCTGCTCAAC TTGACCAAGA AACCTCTGGA AAGCTCAACA ACGAAAAGCT TGCCAAGCAG 
TTCGAACTTC AACTCACCGA GCTTCAATCT AAGGCTGATG AGCAATCTCG CCAACTCCAA 
GACTTCACTT CCCTTAAGGG ACGCCTTCAC TCTGAGAATG GAGACCTTGT CCGTCAACTC 
GAAGATGCCG AATCCCAAGT CAACCAACTC ACCAGACTGA AGTCCCAGCT TACTTCTCAA 
CTCGAGGAGG CTCGCCGCAC CGCTGACGAG GAAGCACGCG AACGTCAAAC CGTCGCCGCC 
CAGGCTAAGA ACTACCAACA CGAAGCCGAG CAACTCCAAG AGTCCCTTGA AGAGGAGATC 
GAAGGAAAGA ACGAGATCCT CAGACAACTC TCCAAGGCTA ACGCCGACAT CCAACAATGG 
AAGGCTCGCT TCGAGGGAGA AGGACTCCTC AAGGCCGACG AGCTCGAGGA TGCCAAGAGA 
CGCCAAGCCC AAAAGATCAA CGAGCTCCAA GAGGCTCTTG ATGCCGCCAA CTCTAAGAAC 
GCTTCTCTTG AGAAGACCAA GTCCAGACTT GTCGGAGACT TGGACGACGC TCAAGTTGAT 
GTCGAGAGAG CCAACGGTGT CGCCAGTGCT CTTGAGAAGA AGCAAAAGGG CTTCGACAAG 
ATCATCGACG AATGGAGAAA GAAGACCGAC GACTTGGCCG CTGAGCTTGA TGGAGCTCAA 
CGCGACCTTC GCAACACCTC CACTGATCTC TTCAAGGCCA AGAACGCCCA AGAGGAGCTC 
GCCGAGGTTG TTGAGGGACT CCGCCGTGAG AACAAGAGCT TGAGCCAAGA GATCAAGGAT 
CTTACCGATC AACTCGGAGA GGGAGGACGC TCTGTCCACG AAATGCAAAA GATCATCCGC 
CGTCTTGAGA TTGAGAAGGA AGAACTCCAA CACGCTTTGG ACGAGGCTGA GGCTGCCCTT 
GAAGCTGAAG AGAGCAAGGT TCTCCGCGCC CAGGTTGAAG TTTCCCAGAT CCGTTCCGAA 
ATCGAGAAAC GCATCCAGGA GAAGGAGGAA GAGTTCGAGA ACACGAGAAA GAACCACGCC 
CGCGCTCTTG AATCAATGCA AGCTTCCCTC GAGACCGAAG CTAAAGGAAA GGCCGAACTT 
CTCCGCATCA AGAAGAAGCT CGAGGGAGAT ATCAACGAGC TCGAGATCGC TTTGGACCAC 
GCCAACAAGG CTAACGCCGA TGCCCAGAAG AACTTGAAGA GATACCAAGA GCAAGTCCGC 
GAGTTGCAAT TGCAAGTCGA GGAGGAGCAA CGCAATGGAG CCGACACCCG TGAACAATTC 
TTCAACGCCG AGAAGCGCGC CACTCTTCTT CAATCCGAGA AGGAAGAACT TTTGGTTGCC 
AACGAGGCCG CCGAGAGAGC CCGCAAGCAA GCCGAGTATG AAGCCGCCGA TGCTCGTGAT 
CAAGCCAACG AAGCCAACGC TCAAGTCAGC AGCTTGACTT CTGCCAAGAG AAAGCTCGAG 
GGAGAAATCC AAGCCATTCA TGCCGACCTC GATGAGACCC TCAACGAGTA CAAGGCCGCT 
GAGGAGCGCT CCAAGAAGGC TATTGCTGAT GCCACCAGAC TCGCAGAGGA GCTCCGTCAA 
GAACAAGAAC ACTCTCAGCA CGTTGACCGT CTTCGCAAGG GACTTGAGCA ACAGCTCAAG 
GAGATCCAAG TCCGTCTTGA TGAGGCCGAG GCTGCTGCTC TTAAGGGAGG AAAGAAGGTT 
ATCGCCAAGC TCGAACAACG CGTGCGTGAG CTCGAATCTG AATTGGACGG AGAACAACGC 
CGCTTCCAGG ATGCCAACAA GAACCTCGGA CGCGCCGATA GACGTGTTCG TGAGCTTCAA 
TTCCAGGTTG ATGAGGACAA GAAGAACTTC GAACGTCTCC AAGATCTCAT TGACAAGCTC 
CAACAGAAGT TGAAGACCCA GAAGAAGCAG GTTGAGGAAG CTGAGGAACT CGCCAACTTG 
AACCTCCAGA AGTACAAGCA ACTCACCCAC CAACTTGAAG ATGCTGAGGA GCGCGCCGAT 
CAAGCCGAAA ACTCCCTCTC AAAGATGCGA TCAAAATCCC GTGCATCCGC CTCTGTTGCC 
CCAGGACTCC AGAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC 
TCTGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-54 ( NM_061195.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493596.1 Caenorhabditis elegans Myosin-4 (unc-54), partial mRNA.

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CloneID	OCa335763
Clone ID Related Accession (Same CDS sequence)	NM_061195.5 , NM_061195.4
Accession Version	NM_061195.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Myosin-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_061195.3. COMPLETENESS: incomplete on both ends.

ATGGAGCACG AGAAGGACCC AGGATGGCAA TATCTCCGCC GTACCAGAGA GCAGGTTTTG 
GAGGATCAAT CTAAGCCTTA TGACTCCAAG AAGAACGTCT GGATCCCAGA TCCAGAAGAG 
GGATACCTTG CCGGAGAAAT CACCGCCACC AAGGGAGACC AGGTCACCAT CGTCACCGCT 
CGCGGAAATG AGGTCACCCT CAAGAAGGAG TTGGTTCAAG AAATGAATCC ACCAAAGTTC 
GAGAAGACCG AAGACATGTC CAACTTGTCT TTCCTCAACG ACGCCTCTGT CCTCCATAAC 
TTGCGTTCCC GTTACGCTGC TATGCTCATC TACACCTACT CCGGACTTTT CTGCGTTGTC 
ATCAACCCAT ACAAGCGTCT CCCAATCTAC ACCGACTCTT GCGCCCGTAT GTTCATGGGA 
AAGAGAAAGA CAGAAATGCC ACCACATTTG TTCGCTGTCT CTGACGAAGC CTACCGTAAC 
ATGCTCCAAG ACCACGAGAA CCAGTCTATG CTCATTACCG GAGAATCTGG AGCCGGAAAG 
ACTGAGAACA CCAAGAAGGT TATTTGCTAC TTCGCCGCTG TCGGTGCCTC CCAACAGGAA 
GGTGGAGCTG AAGTTGATCC AAATAAGAAG AAGGTCACCC TTGAGGACCA AATCGTCCAG 
ACCAATCCAG TACTTGAAGC CTTCGGTAAC GCCAAGACTG TCCGTAACAA CAACTCATCC 
CGTTTCGGAA AGTTCATCCG TATCCACTTC AACAAGCACG GACGCCTCGC ATCTTGCGAT 
ATCGAGCATT ACTTGCTCGA GAAATCTCGT GTCATCCGTC AAGCTCCAGG AGAGCGTTGC 
TACCACATCT TCTACCAAAT CTACTCTGAC TTCCGTCCAG AGCTCAAGAA GGAACTTCTT 
CTTGACTTGC CAATCAAGGA TTACTGGTTC GTCGCCCAGG CCGAGTTGAT CATTGACGGA 
ATCGATGACG TCGAAGAGTT CCAACTTACT GATGAAGCTT TCGACATCCT CAACTTCTCT 
GCCGTCGAGA AGCAGGATTG CTACCGCCTC ATGTCCGCTC ACATGCACAT GGGTAACATG 
AAGTTCAAGC AACGCCCACG TGAAGAGCAA GCTGAGCCAG ATGGTACCGA TGAGGCCGAG 
AAGGCATCCA ACATGTACGG AATCGGATGC GAGGAGTTCC TCAAGGCTTT GACCAAGCCA 
CGTGTCAAGG TCGGAACCGA ATGGGTATCC AAGGGACAGA ACTGCGAACA AGTCAATTGG 
GCTGTCGGAG CCATGGCCAA GGGTCTCTAC TCCCGTGTGT TCAACTGGCT TGTCAAGAAG 
TGTAACCTCA CTCTGGATCA AAAGGGTATT GATCGTGATT ATTTCATCGG TGTGCTCGAT 
ATCGCCGGTT TCGAAATCTT CGACTTCAAC TCCTTCGAGC AGTTGTGGAT TAACTTCGTA 
AACGAGAAGC TCCAACAATT CTTCAACCAT CACATGTTCG TCCTTGAACA GGAAGAATAC 
GCCCGTGAGG GTATTCAATG GGTCTTCATC GATTTCGGAC TTGATTTGCA AGCGTGTATC 
GAACTTATTG AGAAGCCACT CGGTATTATC TCCATGCTTG ATGAAGAGTG TATCGTACCA 
AAGGCTACTG ATTTGACCCT CGCTTCCAAG CTTGTCGATC AACATCTTGG CAAGCATCCA 
AACTTCGAGA AGCCAAAGCC ACCAAAGGGA AAGCAAGGAG AAGCTCACTT CGCCATGCGG 
CACTACGCCG GAACTGTGCG TTACAACTGC TTGAACTGGC TCGAGAAGAA CAAGGACCCC 
CTCAACGACA CCGTTGTCTC GGCAATGAAA CAATCCAAGG GAAACGATCT TCTCGTCGAG 
ATCTGGCAAG ACTACACCAC CCAGGAAGAG GCCGCCGCTA AGGCCAAGGA GGGAGGTGGC 
GGTGGAAAGA AGAAGGGAAA GTCCGGATCT TTCATGACCG TCTCTATGCT CTACAGAGAG 
TCTCTCAACA ACTTGATGAC TATGCTCAAC AAGACTCACC CACATTTCAT CCGTTGTATC 
ATCCCCAACG AGAAGAAGCA ATCTGGTATG ATCGATGCCG CTTTGGTTCT CAACCAGCTT 
ACCTGCAACG GAGTGTTGGA AGGAATCAGA ATTTGCAGAA AGGGATTCCC CAACAGAACC 
CTTCATCCAG ACTTCGTCCA ACGTTACGCC ATCCTTGCCG CCAAGGAGGC CAAGTCCGAT 
GACGACAAGA AGAAGTGCGC CGAGGCTATC ATGTCCAAGC TCGTCAACGA CGGATCCCTC 
AGCGAGGAGA TGTTCCGTAT CGGTCTCACC AAGGTCTTCT TCAAGGCTGG AGTTCTTGCT 
CATCTTGAAG ACATCCGTGA CGAGAAGCTC GCCACCATCC TCACCGGATT CCAATCCCAA 
ATCAGATGGC ATTTGGGTCT CAAGGACCGC AAGCGCCGTA TGGAACAACG TGCCGGACTT 
CTCATTGTTC AGCGCAACGT CCGTTCCTGG TGCACTCTCC GTACCTGGGA ATGGTTCAAG 
CTTTACGGAA AGGTCAAGCC AATGCTCAAG GCCGGAAAGG AAGCCGAGGA GCTCGAGAAG 
ATCAACGACA AGGTTAAGGC CCTCGAAGAC AGTCTCGCCA AGGAGGAGAA GCTTCGCAAG 
GAGCTCGAGG AGTCCTCTGC CAAGCTCGTC GAAGAGAAAA CTTCTCTCTT CACCAACTTG 
GAATCCACCA AGACTCAACT CTCCGATGCT GAGGAAAGAC TTGCCAAGCT TGAGGCTCAA 
CAAAAGGATG CCTCCAAGCA ACTCTCCGAG CTCAACGATC AACTCGCTGA CAACGAAGAC 
CGTACCGCTG ATGTTCAGCG CGCTAAGAAG AAGATCGAGG CCGAGGTCGA GGCTTTGAAG 
AAGCAGATCC AAGACTTGGA AATGTCTCTC CGCAAGGCTG AGTCTGAGAA GCAATCCAAG 
GATCACCAGA TCAGATCCCT TCAAGATGAG ATGCAACAGC AAGATGAAGC TATTGCCAAG 
CTCAACAAGG AGAAGAAGCA TCAAGAAGAA ATCAACCGCA AGCTGATGGA GGACCTCCAA 
TCCGAAGAGG ATAAGGGTAA CCATCAGAAC AAGGTCAAGG CCAAGCTTGA GCAGACTCTT 
GATGACCTCG AGGATTCCCT TGAGCGCGAG AAGAGAGCCC GCGCCGATCT TGACAAGCAG 
AAGAGAAAGG TCGAGGGAGA GCTCAAGATT GCTCAAGAGA ACATCGATGA GAGCGGACGC 
CAACGCCACG ATCTTGAGAA CAACTTGAAG AAGAAGGAAT CTGAGTTGCA CTCAGTTTCT 
TCCCGACTTG AGGACGAACA AGCTCTTGTC TCCAAGCTCC AACGCCAGAT CAAGGACGGA 
CAAAGCCGTA TCTCCGAGCT CGAAGAGGAA CTCGAGAATG AACGTCAATC CCGTTCCAAG 
GCTGACCGTG CCAAGAGCGA CCTCCAACGC GAGTTGGAAG AGCTCGGTGA GAAGCTTGAC 
GAGCAAGGTG GAGCTACTGC CGCCCAGGTT GAGGTCAACA AGAAGCGTGA GGCTGAACTT 
GCCAAGCTCC GCAGAGACTT GGAGGAGGCC AACATGAACC ACGAGAACCA ACTCGGTGGA 
CTTCGCAAGA AGCACACCGA CGCTGTCGCT GAGCTCACCG ACCAACTCGA TCAACTCAAC 
AAGGCCAAGG CTAAGGTCGA GAAGGACAAG GCTCAAGCTG TTCGTGACGC TGAGGACCTT 
GCTGCTCAAC TTGACCAAGA AACCTCTGGA AAGCTCAACA ACGAAAAGCT TGCCAAGCAG 
TTCGAACTTC AACTCACCGA GCTTCAATCT AAGGCTGATG AGCAATCTCG CCAACTCCAA 
GACTTCACTT CCCTTAAGGG ACGCCTTCAC TCTGAGAATG GAGACCTTGT CCGTCAACTC 
GAAGATGCCG AATCCCAAGT CAACCAACTC ACCAGACTGA AGTCCCAGCT TACTTCTCAA 
CTCGAGGAGG CTCGCCGCAC CGCTGACGAG GAAGCACGCG AACGTCAAAC CGTCGCCGCC 
CAGGCTAAGA ACTACCAACA CGAAGCCGAG CAACTCCAAG AGTCCCTTGA AGAGGAGATC 
GAAGGAAAGA ACGAGATCCT CAGACAACTC TCCAAGGCTA ACGCCGACAT CCAACAATGG 
AAGGCTCGCT TCGAGGGAGA AGGACTCCTC AAGGCCGACG AGCTCGAGGA TGCCAAGAGA 
CGCCAAGCCC AAAAGATCAA CGAGCTCCAA GAGGCTCTTG ATGCCGCCAA CTCTAAGAAC 
GCTTCTCTTG AGAAGACCAA GTCCAGACTT GTCGGAGACT TGGACGACGC TCAAGTTGAT 
GTCGAGAGAG CCAACGGTGT CGCCAGTGCT CTTGAGAAGA AGCAAAAGGG CTTCGACAAG 
ATCATCGACG AATGGAGAAA GAAGACCGAC GACTTGGCCG CTGAGCTTGA TGGAGCTCAA 
CGCGACCTTC GCAACACCTC CACTGATCTC TTCAAGGCCA AGAACGCCCA AGAGGAGCTC 
GCCGAGGTTG TTGAGGGACT CCGCCGTGAG AACAAGAGCT TGAGCCAAGA GATCAAGGAT 
CTTACCGATC AACTCGGAGA GGGAGGACGC TCTGTCCACG AAATGCAAAA GATCATCCGC 
CGTCTTGAGA TTGAGAAGGA AGAACTCCAA CACGCTTTGG ACGAGGCTGA GGCTGCCCTT 
GAAGCTGAAG AGAGCAAGGT TCTCCGCGCC CAGGTTGAAG TTTCCCAGAT CCGTTCCGAA 
ATCGAGAAAC GCATCCAGGA GAAGGAGGAA GAGTTCGAGA ACACGAGAAA GAACCACGCC 
CGCGCTCTTG AATCAATGCA AGCTTCCCTC GAGACCGAAG CTAAAGGAAA GGCCGAACTT 
CTCCGCATCA AGAAGAAGCT CGAGGGAGAT ATCAACGAGC TCGAGATCGC TTTGGACCAC 
GCCAACAAGG CTAACGCCGA TGCCCAGAAG AACTTGAAGA GATACCAAGA GCAAGTCCGC 
GAGTTGCAAT TGCAAGTCGA GGAGGAGCAA CGCAATGGAG CCGACACCCG TGAACAATTC 
TTCAACGCCG AGAAGCGCGC CACTCTTCTT CAATCCGAGA AGGAAGAACT TTTGGTTGCC 
AACGAGGCCG CCGAGAGAGC CCGCAAGCAA GCCGAGTATG AAGCCGCCGA TGCTCGTGAT 
CAAGCCAACG AAGCCAACGC TCAAGTCAGC AGCTTGACTT CTGCCAAGAG AAAGCTCGAG 
GGAGAAATCC AAGCCATTCA TGCCGACCTC GATGAGACCC TCAACGAGTA CAAGGCCGCT 
GAGGAGCGCT CCAAGAAGGC TATTGCTGAT GCCACCAGAC TCGCAGAGGA GCTCCGTCAA 
GAACAAGAAC ACTCTCAGCA CGTTGACCGT CTTCGCAAGG GACTTGAGCA ACAGCTCAAG 
GAGATCCAAG TCCGTCTTGA TGAGGCCGAG GCTGCTGCTC TTAAGGGAGG AAAGAAGGTT 
ATCGCCAAGC TCGAACAACG CGTGCGTGAG CTCGAATCTG AATTGGACGG AGAACAACGC 
CGCTTCCAGG ATGCCAACAA GAACCTCGGA CGCGCCGATA GACGTGTTCG TGAGCTTCAA 
TTCCAGGTTG ATGAGGACAA GAAGAACTTC GAACGTCTCC AAGATCTCAT TGACAAGCTC 
CAACAGAAGT TGAAGACCCA GAAGAAGCAG GTTGAGGAAG CTGAGGAACT CGCCAACTTG 
AACCTCCAGA AGTACAAGCA ACTCACCCAC CAACTTGAAG ATGCTGAGGA GCGCGCCGAT 
CAAGCCGAAA ACTCCCTCTC AAAGATGCGA TCAAAATCCC GTGCATCCGC CTCTGTTGCC 
CCAGGACTCC AGAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC 
TCTGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493596.1
CDS	1..5892
Translation	MEHEKDPGWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTARGNEVTLKKELVQEMNPPKFEKTEDMSNLSFLNDASVLHNLRSRYAAMLIYTYSGLFCVVINPYKRLPIYTDSCARMFMGKRKTEMPPHLFAVSDEAYRNMLQDHENQSMLITGESGAGKTENTKKVICYFAAVGASQQEGGAEVDPNKKKVTLEDQIVQTNPVLEAFGNAKTVRNNNSSRFGKFIRIHFNKHGRLASCDIEHYLLEKSRVIRQAPGERCYHIFYQIYSDFRPELKKELLLDLPIKDYWFVAQAELIIDGIDDVEEFQLTDEAFDILNFSAVEKQDCYRLMSAHMHMGNMKFKQRPREEQAEPDGTDEAEKASNMYGIGCEEFLKALTKPRVKVGTEWVSKGQNCEQVNWAVGAMAKGLYSRVFNWLVKKCNLTLDQKGIDRDYFIGVLDIAGFEIFDFNSFEQLWINFVNEKLQQFFNHHMFVLEQEEYAREGIQWVFIDFGLDLQACIELIEKPLGIISMLDEECIVPKATDLTLASKLVDQHLGKHPNFEKPKPPKGKQGEAHFAMRHYAGTVRYNCLNWLEKNKDPLNDTVVSAMKQSKGNDLLVEIWQDYTTQEEAAAKAKEGGGGGKKKGKSGSFMTVSMLYRESLNNLMTMLNKTHPHFIRCIIPNEKKQSGMIDAALVLNQLTCNGVLEGIRICRKGFPNRTLHPDFVQRYAILAAKEAKSDDDKKKCAEAIMSKLVNDGSLSEEMFRIGLTKVFFKAGVLAHLEDIRDEKLATILTGFQSQIRWHLGLKDRKRRMEQRAGLLIVQRNVRSWCTLRTWEWFKLYGKVKPMLKAGKEAEELEKINDKVKALEDSLAKEEKLRKELEESSAKLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKDASKQLSELNDQLADNEDRTADVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKDHQIRSLQDEMQQQDEAIAKLNKEKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQTLDDLEDSLEREKRARADLDKQKRKVEGELKIAQENIDESGRQRHDLENNLKKKESELHSVSSRLEDEQALVSKLQRQIKDGQSRISELEEELENERQSRSKADRAKSDLQRELEELGEKLDEQGGATAAQVEVNKKREAELAKLRRDLEEANMNHENQLGGLRKKHTDAVAELTDQLDQLNKAKAKVEKDKAQAVRDAEDLAAQLDQETSGKLNNEKLAKQFELQLTELQSKADEQSRQLQDFTSLKGRLHSENGDLVRQLEDAESQVNQLTRLKSQLTSQLEEARRTADEEARERQTVAAQAKNYQHEAEQLQESLEEEIEGKNEILRQLSKANADIQQWKARFEGEGLLKADELEDAKRRQAQKINELQEALDAANSKNASLEKTKSRLVGDLDDAQVDVERANGVASALEKKQKGFDKIIDEWRKKTDDLAAELDGAQRDLRNTSTDLFKAKNAQEELAEVVEGLRRENKSLSQEIKDLTDQLGEGGRSVHEMQKIIRRLEIEKEELQHALDEAEAALEAEESKVLRAQVEVSQIRSEIEKRIQEKEEEFENTRKNHARALESMQASLETEAKGKAELLRIKKKLEGDINELEIALDHANKANADAQKNLKRYQEQVRELQLQVEEEQRNGADTREQFFNAEKRATLLQSEKEELLVANEAAERARKQAEYEAADARDQANEANAQVSSLTSAKRKLEGEIQAIHADLDETLNEYKAAEERSKKAIADATRLAEELRQEQEHSQHVDRLRKGLEQQLKEIQVRLDEAEAAALKGGKKVIAKLEQRVRELESELDGEQRRFQDANKNLGRADRRVRELQFQVDEDKKNFERLQDLIDKLQQKLKTQKKQVEEAEELANLNLQKYKQLTHQLEDAEERADQAENSLSKMRSKSRASASVAPGLQSSASAAVIRSPSRARASDF

Target ORF information:

RefSeq Version	NM_061195.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Myosin-4 (unc-54), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061195.4

ORF Insert Sequence:

ATGGAGCACG AGAAGGACCC AGGATGGCAA TATCTCCGCC GTACCAGAGA GCAGGTTTTG 
GAGGATCAAT CTAAGCCTTA TGACTCCAAG AAGAACGTCT GGATCCCAGA TCCAGAAGAG 
GGATACCTTG CCGGAGAAAT CACCGCCACC AAGGGAGACC AGGTCACCAT CGTCACCGCT 
CGCGGAAATG AGGTCACCCT CAAGAAGGAG TTGGTTCAAG AAATGAATCC ACCAAAGTTC 
GAGAAGACCG AAGACATGTC CAACTTGTCT TTCCTCAACG ACGCCTCTGT CCTCCATAAC 
TTGCGTTCCC GTTACGCTGC TATGCTCATC TACACCTACT CCGGACTTTT CTGCGTTGTC 
ATCAACCCAT ACAAGCGTCT CCCAATCTAC ACCGACTCTT GCGCCCGTAT GTTCATGGGA 
AAGAGAAAGA CAGAAATGCC ACCACATTTG TTCGCTGTCT CTGACGAAGC CTACCGTAAC 
ATGCTCCAAG ACCACGAGAA CCAGTCTATG CTCATTACCG GAGAATCTGG AGCCGGAAAG 
ACTGAGAACA CCAAGAAGGT TATTTGCTAC TTCGCCGCTG TCGGTGCCTC CCAACAGGAA 
GGTGGAGCTG AAGTTGATCC AAATAAGAAG AAGGTCACCC TTGAGGACCA AATCGTCCAG 
ACCAATCCAG TACTTGAAGC CTTCGGTAAC GCCAAGACTG TCCGTAACAA CAACTCATCC 
CGTTTCGGAA AGTTCATCCG TATCCACTTC AACAAGCACG GACGCCTCGC ATCTTGCGAT 
ATCGAGCATT ACTTGCTCGA GAAATCTCGT GTCATCCGTC AAGCTCCAGG AGAGCGTTGC 
TACCACATCT TCTACCAAAT CTACTCTGAC TTCCGTCCAG AGCTCAAGAA GGAACTTCTT 
CTTGACTTGC CAATCAAGGA TTACTGGTTC GTCGCCCAGG CCGAGTTGAT CATTGACGGA 
ATCGATGACG TCGAAGAGTT CCAACTTACT GATGAAGCTT TCGACATCCT CAACTTCTCT 
GCCGTCGAGA AGCAGGATTG CTACCGCCTC ATGTCCGCTC ACATGCACAT GGGTAACATG 
AAGTTCAAGC AACGCCCACG TGAAGAGCAA GCTGAGCCAG ATGGTACCGA TGAGGCCGAG 
AAGGCATCCA ACATGTACGG AATCGGATGC GAGGAGTTCC TCAAGGCTTT GACCAAGCCA 
CGTGTCAAGG TCGGAACCGA ATGGGTATCC AAGGGACAGA ACTGCGAACA AGTCAATTGG 
GCTGTCGGAG CCATGGCCAA GGGTCTCTAC TCCCGTGTGT TCAACTGGCT TGTCAAGAAG 
TGTAACCTCA CTCTGGATCA AAAGGGTATT GATCGTGATT ATTTCATCGG TGTGCTCGAT 
ATCGCCGGTT TCGAAATCTT CGACTTCAAC TCCTTCGAGC AGTTGTGGAT TAACTTCGTA 
AACGAGAAGC TCCAACAATT CTTCAACCAT CACATGTTCG TCCTTGAACA GGAAGAATAC 
GCCCGTGAGG GTATTCAATG GGTCTTCATC GATTTCGGAC TTGATTTGCA AGCGTGTATC 
GAACTTATTG AGAAGCCACT CGGTATTATC TCCATGCTTG ATGAAGAGTG TATCGTACCA 
AAGGCTACTG ATTTGACCCT CGCTTCCAAG CTTGTCGATC AACATCTTGG CAAGCATCCA 
AACTTCGAGA AGCCAAAGCC ACCAAAGGGA AAGCAAGGAG AAGCTCACTT CGCCATGCGG 
CACTACGCCG GAACTGTGCG TTACAACTGC TTGAACTGGC TCGAGAAGAA CAAGGACCCC 
CTCAACGACA CCGTTGTCTC GGCAATGAAA CAATCCAAGG GAAACGATCT TCTCGTCGAG 
ATCTGGCAAG ACTACACCAC CCAGGAAGAG GCCGCCGCTA AGGCCAAGGA GGGAGGTGGC 
GGTGGAAAGA AGAAGGGAAA GTCCGGATCT TTCATGACCG TCTCTATGCT CTACAGAGAG 
TCTCTCAACA ACTTGATGAC TATGCTCAAC AAGACTCACC CACATTTCAT CCGTTGTATC 
ATCCCCAACG AGAAGAAGCA ATCTGGTATG ATCGATGCCG CTTTGGTTCT CAACCAGCTT 
ACCTGCAACG GAGTGTTGGA AGGAATCAGA ATTTGCAGAA AGGGATTCCC CAACAGAACC 
CTTCATCCAG ACTTCGTCCA ACGTTACGCC ATCCTTGCCG CCAAGGAGGC CAAGTCCGAT 
GACGACAAGA AGAAGTGCGC CGAGGCTATC ATGTCCAAGC TCGTCAACGA CGGATCCCTC 
AGCGAGGAGA TGTTCCGTAT CGGTCTCACC AAGGTCTTCT TCAAGGCTGG AGTTCTTGCT 
CATCTTGAAG ACATCCGTGA CGAGAAGCTC GCCACCATCC TCACCGGATT CCAATCCCAA 
ATCAGATGGC ATTTGGGTCT CAAGGACCGC AAGCGCCGTA TGGAACAACG TGCCGGACTT 
CTCATTGTTC AGCGCAACGT CCGTTCCTGG TGCACTCTCC GTACCTGGGA ATGGTTCAAG 
CTTTACGGAA AGGTCAAGCC AATGCTCAAG GCCGGAAAGG AAGCCGAGGA GCTCGAGAAG 
ATCAACGACA AGGTTAAGGC CCTCGAAGAC AGTCTCGCCA AGGAGGAGAA GCTTCGCAAG 
GAGCTCGAGG AGTCCTCTGC CAAGCTCGTC GAAGAGAAAA CTTCTCTCTT CACCAACTTG 
GAATCCACCA AGACTCAACT CTCCGATGCT GAGGAAAGAC TTGCCAAGCT TGAGGCTCAA 
CAAAAGGATG CCTCCAAGCA ACTCTCCGAG CTCAACGATC AACTCGCTGA CAACGAAGAC 
CGTACCGCTG ATGTTCAGCG CGCTAAGAAG AAGATCGAGG CCGAGGTCGA GGCTTTGAAG 
AAGCAGATCC AAGACTTGGA AATGTCTCTC CGCAAGGCTG AGTCTGAGAA GCAATCCAAG 
GATCACCAGA TCAGATCCCT TCAAGATGAG ATGCAACAGC AAGATGAAGC TATTGCCAAG 
CTCAACAAGG AGAAGAAGCA TCAAGAAGAA ATCAACCGCA AGCTGATGGA GGACCTCCAA 
TCCGAAGAGG ATAAGGGTAA CCATCAGAAC AAGGTCAAGG CCAAGCTTGA GCAGACTCTT 
GATGACCTCG AGGATTCCCT TGAGCGCGAG AAGAGAGCCC GCGCCGATCT TGACAAGCAG 
AAGAGAAAGG TCGAGGGAGA GCTCAAGATT GCTCAAGAGA ACATCGATGA GAGCGGACGC 
CAACGCCACG ATCTTGAGAA CAACTTGAAG AAGAAGGAAT CTGAGTTGCA CTCAGTTTCT 
TCCCGACTTG AGGACGAACA AGCTCTTGTC TCCAAGCTCC AACGCCAGAT CAAGGACGGA 
CAAAGCCGTA TCTCCGAGCT CGAAGAGGAA CTCGAGAATG AACGTCAATC CCGTTCCAAG 
GCTGACCGTG CCAAGAGCGA CCTCCAACGC GAGTTGGAAG AGCTCGGTGA GAAGCTTGAC 
GAGCAAGGTG GAGCTACTGC CGCCCAGGTT GAGGTCAACA AGAAGCGTGA GGCTGAACTT 
GCCAAGCTCC GCAGAGACTT GGAGGAGGCC AACATGAACC ACGAGAACCA ACTCGGTGGA 
CTTCGCAAGA AGCACACCGA CGCTGTCGCT GAGCTCACCG ACCAACTCGA TCAACTCAAC 
AAGGCCAAGG CTAAGGTCGA GAAGGACAAG GCTCAAGCTG TTCGTGACGC TGAGGACCTT 
GCTGCTCAAC TTGACCAAGA AACCTCTGGA AAGCTCAACA ACGAAAAGCT TGCCAAGCAG 
TTCGAACTTC AACTCACCGA GCTTCAATCT AAGGCTGATG AGCAATCTCG CCAACTCCAA 
GACTTCACTT CCCTTAAGGG ACGCCTTCAC TCTGAGAATG GAGACCTTGT CCGTCAACTC 
GAAGATGCCG AATCCCAAGT CAACCAACTC ACCAGACTGA AGTCCCAGCT TACTTCTCAA 
CTCGAGGAGG CTCGCCGCAC CGCTGACGAG GAAGCACGCG AACGTCAAAC CGTCGCCGCC 
CAGGCTAAGA ACTACCAACA CGAAGCCGAG CAACTCCAAG AGTCCCTTGA AGAGGAGATC 
GAAGGAAAGA ACGAGATCCT CAGACAACTC TCCAAGGCTA ACGCCGACAT CCAACAATGG 
AAGGCTCGCT TCGAGGGAGA AGGACTCCTC AAGGCCGACG AGCTCGAGGA TGCCAAGAGA 
CGCCAAGCCC AAAAGATCAA CGAGCTCCAA GAGGCTCTTG ATGCCGCCAA CTCTAAGAAC 
GCTTCTCTTG AGAAGACCAA GTCCAGACTT GTCGGAGACT TGGACGACGC TCAAGTTGAT 
GTCGAGAGAG CCAACGGTGT CGCCAGTGCT CTTGAGAAGA AGCAAAAGGG CTTCGACAAG 
ATCATCGACG AATGGAGAAA GAAGACCGAC GACTTGGCCG CTGAGCTTGA TGGAGCTCAA 
CGCGACCTTC GCAACACCTC CACTGATCTC TTCAAGGCCA AGAACGCCCA AGAGGAGCTC 
GCCGAGGTTG TTGAGGGACT CCGCCGTGAG AACAAGAGCT TGAGCCAAGA GATCAAGGAT 
CTTACCGATC AACTCGGAGA GGGAGGACGC TCTGTCCACG AAATGCAAAA GATCATCCGC 
CGTCTTGAGA TTGAGAAGGA AGAACTCCAA CACGCTTTGG ACGAGGCTGA GGCTGCCCTT 
GAAGCTGAAG AGAGCAAGGT TCTCCGCGCC CAGGTTGAAG TTTCCCAGAT CCGTTCCGAA 
ATCGAGAAAC GCATCCAGGA GAAGGAGGAA GAGTTCGAGA ACACGAGAAA GAACCACGCC 
CGCGCTCTTG AATCAATGCA AGCTTCCCTC GAGACCGAAG CTAAAGGAAA GGCCGAACTT 
CTCCGCATCA AGAAGAAGCT CGAGGGAGAT ATCAACGAGC TCGAGATCGC TTTGGACCAC 
GCCAACAAGG CTAACGCCGA TGCCCAGAAG AACTTGAAGA GATACCAAGA GCAAGTCCGC 
GAGTTGCAAT TGCAAGTCGA GGAGGAGCAA CGCAATGGAG CCGACACCCG TGAACAATTC 
TTCAACGCCG AGAAGCGCGC CACTCTTCTT CAATCCGAGA AGGAAGAACT TTTGGTTGCC 
AACGAGGCCG CCGAGAGAGC CCGCAAGCAA GCCGAGTATG AAGCCGCCGA TGCTCGTGAT 
CAAGCCAACG AAGCCAACGC TCAAGTCAGC AGCTTGACTT CTGCCAAGAG AAAGCTCGAG 
GGAGAAATCC AAGCCATTCA TGCCGACCTC GATGAGACCC TCAACGAGTA CAAGGCCGCT 
GAGGAGCGCT CCAAGAAGGC TATTGCTGAT GCCACCAGAC TCGCAGAGGA GCTCCGTCAA 
GAACAAGAAC ACTCTCAGCA CGTTGACCGT CTTCGCAAGG GACTTGAGCA ACAGCTCAAG 
GAGATCCAAG TCCGTCTTGA TGAGGCCGAG GCTGCTGCTC TTAAGGGAGG AAAGAAGGTT 
ATCGCCAAGC TCGAACAACG CGTGCGTGAG CTCGAATCTG AATTGGACGG AGAACAACGC 
CGCTTCCAGG ATGCCAACAA GAACCTCGGA CGCGCCGATA GACGTGTTCG TGAGCTTCAA 
TTCCAGGTTG ATGAGGACAA GAAGAACTTC GAACGTCTCC AAGATCTCAT TGACAAGCTC 
CAACAGAAGT TGAAGACCCA GAAGAAGCAG GTTGAGGAAG CTGAGGAACT CGCCAACTTG 
AACCTCCAGA AGTACAAGCA ACTCACCCAC CAACTTGAAG ATGCTGAGGA GCGCGCCGAT 
CAAGCCGAAA ACTCCCTCTC AAAGATGCGA TCAAAATCCC GTGCATCCGC CTCTGTTGCC 
CCAGGACTCC AGAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC 
TCTGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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unc-54 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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