ego-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ego-2
Entrez Gene ID	190251
Full Name	Enhancer of Glp-One (glp-1)
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083154.2	NP_001076623.1	Enhancer of Glp-One (glp-1)
NM_001264705.2	NP_001251634.1	BRO1 domain-containing protein
NM_001083154.3	NP_001076623.1	BRO1 domain-containing protein
NM_001083153.3	NP_001076622.2	Enhancer of Glp-One (glp-1)
NM_001264705.1	NP_001251634.1	Enhancer of Glp-One (glp-1)
NM_001083153.4	NP_001076622.2	BRO1 domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ego-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ego-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335487	NM_001083154.3 Latest version!	Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OCa335486	NM_001083153.4 Latest version!	Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa336093	NM_001264705.2 Latest version!	Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OCa335487	NM_001083154.2	Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OCa336093	NM_001264705.1	Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCa335486	NM_001083153.3	Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ego-2 ( NM_001083154.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076623.1 Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.

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CloneID	OCa335487
Clone ID Related Accession (Same CDS sequence)	NM_001083154.2 , NM_001083154.3
Accession Version	NM_001083154.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Enhancer of Glp-One (glp-1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001083154.1. COMPLETENESS: incomplete on both ends.

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGAAA CCAAGGACCG CCTCCACCAA TGAATCAATC 
GGATCCACTC AATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076623.1
CDS	1..801
Translation	MEALPIMPMLAPQSRSSTLSYDNIPNFDFRLRMKEYILLTFNADPHDYDSAFDELTQMKFEANVPTPSVEQTLKLKRYYGQLCMMQKRFPMGAGEQLETPFAWHDGLIDIRSAQSEVTICDIEFEKASVMFNIGACHAQYAAEQTRDTQDCIKAAFMHFQYAAYAFEQLNSFRNSDIFYPSVDLDANVISFYYKVMIAQAQECLVQKSLLDNRSAIVIAKLSLWLQEAYDSAAKIVDEWSVNIPESVQRKPRTASTNESIGSTQSN

Target ORF information:

RefSeq Version	NM_001083154.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083154.2

ORF Insert Sequence:

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGAAA CCAAGGACCG CCTCCACCAA TGAATCAATC 
GGATCCACTC AATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ego-2 ( NM_001083154.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076623.1 Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.

Price & Availability↑

CloneID	OCa335487
Clone ID Related Accession (Same CDS sequence)	NM_001083154.2 , NM_001083154.3
Accession Version	NM_001083154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BRO1 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001083154.2.

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGAAA CCAAGGACCG CCTCCACCAA TGAATCAATC 
GGATCCACTC AATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076623.1
CDS	8..808
Translation	MEALPIMPMLAPQSRSSTLSYDNIPNFDFRLRMKEYILLTFNADPHDYDSAFDELTQMKFEANVPTPSVEQTLKLKRYYGQLCMMQKRFPMGAGEQLETPFAWHDGLIDIRSAQSEVTICDIEFEKASVMFNIGACHAQYAAEQTRDTQDCIKAAFMHFQYAAYAFEQLNSFRNSDIFYPSVDLDANVISFYYKVMIAQAQECLVQKSLLDNRSAIVIAKLSLWLQEAYDSAAKIVDEWSVNIPESVQRKPRTASTNESIGSTQSN

Target ORF information:

RefSeq Version	NM_001083154.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083154.3

ORF Insert Sequence:

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGAAA CCAAGGACCG CCTCCACCAA TGAATCAATC 
GGATCCACTC AATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ego-2 ( NM_001083153.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076622.2 Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.

Price & Availability↑

CloneID	OCa335486
Clone ID Related Accession (Same CDS sequence)	NM_001083153.3 , NM_001083153.4
Accession Version	NM_001083153.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BRO1 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001083153.3.

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GACTCCATGG 
AACACTACAC CACAGTATCA TGCTCCGCCA GGAGCTCAAG CTGCTGCTCC AGAAGCCCCC 
GCGGCTCCGG CTCCGGGAGC CCCAGCTGCT CGGAGCAACG TGGATTTGCT GAGCGACCTG 
CTCGGCGACT TTAACTTGCC TCCACAGCCG ATTCAGCCGA CGGTTCACAG CACTCAACAG 
CTCCAGCATC AGGCTCCCCA GCAGCAGGAG ATGTCGGATT TTCGAATTGC CGCCGCCACA 
CTGAATCCGC CGCCGGAATC GGTTAAACCG GTGAAACCGG CAGCCGCTGT TCAGCCAATG 
CCTCAAATGA CGGCTCAACA AGTACCGGCT CACGGAGCAC AAGAGACTCC GATCCTCAAA 
TCGTCTGAGC TTTCGAGCTC GGCGAGCACA AGCAGCAGCA TCACAGAGCT CTCGGATCCG 
AGCAAATTCC AGTTGGGAGT TGGAAATGTG CAGAAGCTGG AAAAACGAAT GCTTCATCAG 
AGTTTTAGAA ACCAAGGACC GCCTCCACCA ATGAATCAAT CGGATCCACT CAATCAAATT 
GATGCATTCA GTAGCTTCAT GACGCCACGT CATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076622.2
CDS	8..4486
Translation	MEALPIMPMLAPQSRSSTLSYDNIPNFDFRLRMKEYILLTFNADPHDYDSAFDELTQMKFEANVPTPSVEQTLKLKRYYGQLCMMQKRFPMGAGEQLETPFAWHDGLIDIRSAQSEVTICDIEFEKASVMFNIGACHAQYAAEQTRDTQDCIKAAFMHFQYAAYAFEQLNSFRNSDIFYPSVDLDANVISFYYKVMIAQAQECLVQKSLLDNRSAIVIAKLSLWLQEAYDSAAKIVDEWSVNIPESVQRYYAKICSLKSAMYAVIAYMSFGDNLEKEDKKMGWRLQYYNIANKYMELLSSNHSKMRERYPELFVTSSFLFDVISAKQRNAEKENDFIYHDRVPKQEDAIDAVQKDGGGAMCKVKTLSFDPLDPSVCGCDLFGKLLPTFVQDAVKKYSDDKDQALREIKECVRSYDEHLNYQLQLAEFDKLRFMLNEGKRSREAWFEISEDLMKRNADMTSYPDCVPNLIDKMGESSDTARVAEAKLNTLLSKLRAIDLQKSSIRSDEGFILIQKELERLAEHLEQAKAHNVSLNKAIAQHSANLQLLTLPCREMWMKIVPPEQQGEMRNGSSPEELQVRKMIEKVMEMQAQRRKLVEQFEADLKADNISNKLMGTNERGAEEIMKSELTKHTNIQQLIRLNITAQDAILRAFADANADFFEERLAMSTKKEEYEKRVSELCASYEVYRDVSKKVDEGEQFYRQLMARCDQFAIPVHAMEEQYREEMEKKERAKKEAEHHLNQIRMSRDAQNALMDFGGGRGGGAPGARAQQQAYQPSRGAAGAGAGAPRGPRLGDFMDSYRARKQAPQAQIPEDQGPPSPTPSSICDFPVQSQRSQRFGAFEGAQGAPGAQRGYQGPHSPVPGALTGSQASGPFGAPPGAPHGYGAPLQAPPQAPYGYDPSRYQDPSTYPAPPPILPGSAAYEHAIRPYPGDTGSYQVPRIVPGAQYGAQAGSGAYFGAQFGAQGAQGAHFGAQGVQFGAQGAQGAHFGAQGAQGAQFGAQGAQGEQFGAQGAQGAHFGAQGAQGAHFGAQGAQGTQFGAQGAQGPPPASYGAPTPPQASYGPAPGAHGYQNGAQGPPGAEVGAQGPPGAHFGAHGASAPPPTSYGAPTPQRPPQASYGAAPGAQGPPGGQFEAHGAAALPPTSHGAPTPQGPFGAAPGAQFGAQGPYGQQGARYEAQKSPGAAIFGAPGAPPQHQGSFGAQFGVPPPQNSAPGAQFGAKPEASSHAPTPPPQPHPSYQAPAPPPALSVFQHSPQGAPITAPPPASSHHEHIAAPQARFTPTPGAPSPWHATPAELKTPWNTTPQYHAPPGAQAAAPEAPAAPAPGAPAARSNVDLLSDLLGDFNLPPQPIQPTVHSTQQLQHQAPQQQEMSDFRIAAATLNPPPESVKPVKPAAAVQPMPQMTAQQVPAHGAQETPILKSSELSSSASTSSSITELSDPSKFQLGVGNVQKLEKRMLHQSFRNQGPPPPMNQSDPLNQIDAFSSFMTPRHQ

Target ORF information:

RefSeq Version	NM_001083153.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083153.4

ORF Insert Sequence:

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GACTCCATGG 
AACACTACAC CACAGTATCA TGCTCCGCCA GGAGCTCAAG CTGCTGCTCC AGAAGCCCCC 
GCGGCTCCGG CTCCGGGAGC CCCAGCTGCT CGGAGCAACG TGGATTTGCT GAGCGACCTG 
CTCGGCGACT TTAACTTGCC TCCACAGCCG ATTCAGCCGA CGGTTCACAG CACTCAACAG 
CTCCAGCATC AGGCTCCCCA GCAGCAGGAG ATGTCGGATT TTCGAATTGC CGCCGCCACA 
CTGAATCCGC CGCCGGAATC GGTTAAACCG GTGAAACCGG CAGCCGCTGT TCAGCCAATG 
CCTCAAATGA CGGCTCAACA AGTACCGGCT CACGGAGCAC AAGAGACTCC GATCCTCAAA 
TCGTCTGAGC TTTCGAGCTC GGCGAGCACA AGCAGCAGCA TCACAGAGCT CTCGGATCCG 
AGCAAATTCC AGTTGGGAGT TGGAAATGTG CAGAAGCTGG AAAAACGAAT GCTTCATCAG 
AGTTTTAGAA ACCAAGGACC GCCTCCACCA ATGAATCAAT CGGATCCACT CAATCAAATT 
GATGCATTCA GTAGCTTCAT GACGCCACGT CATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ego-2 ( NM_001264705.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001251634.1 Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.

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CloneID	OCa336093
Clone ID Related Accession (Same CDS sequence)	NM_001264705.2 , NM_001264705.1
Accession Version	NM_001264705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BRO1 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001264705.1.

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GTTTCAGACT 
CCATGGAACA CTACACCACA GTATCATGCT CCGCCAGGAG CTCAAGCTGC TGCTCCAGAA 
GCCCCCGCGG CTCCGGCTCC GGGAGCCCCA GCTGCTCGGA GCAACGTGGA TTTGCTGAGC 
GACCTGCTCG GCGACTTTAA CTTGCCTCCA CAGCCGATTC AGCCGACGGT TCACAGCACT 
CAACAGCTCC AGCATCAGGC TCCCCAGCAG CAGGAGATGT CGGATTTTCG AATTGCCGCC 
GCCACACTGA ATCCGCCGCC GGAATCGGTT AAACCGGTGA AACCGGCAGC CGCTGTTCAG 
CCAATGCCTC AAATGACGGC TCAACAAGTA CCGGCTCACG GAGCACAAGA GACTCCGATC 
CTCAAATCGT CTGAGCTTTC GAGCTCGGCG AGCACAAGCA GCAGCATCAC AGAGCTCTCG 
GATCCGAGCA AATTCCAGTT GGGAGTTGGA AATGTGCAGA AGCTGGAAAA ACGAATGCTT 
CATCAGAGTT TTAGAAACCA AGGACCGCCT CCACCAATGA ATCAATCGGA TCCACTCAAT 
CAAATTGATG CATTCAGTAG CTTCATGACG CCACGTCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001251634.1
CDS	8..4492
Translation	MEALPIMPMLAPQSRSSTLSYDNIPNFDFRLRMKEYILLTFNADPHDYDSAFDELTQMKFEANVPTPSVEQTLKLKRYYGQLCMMQKRFPMGAGEQLETPFAWHDGLIDIRSAQSEVTICDIEFEKASVMFNIGACHAQYAAEQTRDTQDCIKAAFMHFQYAAYAFEQLNSFRNSDIFYPSVDLDANVISFYYKVMIAQAQECLVQKSLLDNRSAIVIAKLSLWLQEAYDSAAKIVDEWSVNIPESVQRYYAKICSLKSAMYAVIAYMSFGDNLEKEDKKMGWRLQYYNIANKYMELLSSNHSKMRERYPELFVTSSFLFDVISAKQRNAEKENDFIYHDRVPKQEDAIDAVQKDGGGAMCKVKTLSFDPLDPSVCGCDLFGKLLPTFVQDAVKKYSDDKDQALREIKECVRSYDEHLNYQLQLAEFDKLRFMLNEGKRSREAWFEISEDLMKRNADMTSYPDCVPNLIDKMGESSDTARVAEAKLNTLLSKLRAIDLQKSSIRSDEGFILIQKELERLAEHLEQAKAHNVSLNKAIAQHSANLQLLTLPCREMWMKIVPPEQQGEMRNGSSPEELQVRKMIEKVMEMQAQRRKLVEQFEADLKADNISNKLMGTNERGAEEIMKSELTKHTNIQQLIRLNITAQDAILRAFADANADFFEERLAMSTKKEEYEKRVSELCASYEVYRDVSKKVDEGEQFYRQLMARCDQFAIPVHAMEEQYREEMEKKERAKKEAEHHLNQIRMSRDAQNALMDFGGGRGGGAPGARAQQQAYQPSRGAAGAGAGAPRGPRLGDFMDSYRARKQAPQAQIPEDQGPPSPTPSSICDFPVQSQRSQRFGAFEGAQGAPGAQRGYQGPHSPVPGALTGSQASGPFGAPPGAPHGYGAPLQAPPQAPYGYDPSRYQDPSTYPAPPPILPGSAAYEHAIRPYPGDTGSYQVPRIVPGAQYGAQAGSGAYFGAQFGAQGAQGAHFGAQGVQFGAQGAQGAHFGAQGAQGAQFGAQGAQGEQFGAQGAQGAHFGAQGAQGAHFGAQGAQGTQFGAQGAQGPPPASYGAPTPPQASYGPAPGAHGYQNGAQGPPGAEVGAQGPPGAHFGAHGASAPPPTSYGAPTPQRPPQASYGAAPGAQGPPGGQFEAHGAAALPPTSHGAPTPQGPFGAAPGAQFGAQGPYGQQGARYEAQKSPGAAIFGAPGAPPQHQGSFGAQFGVPPPQNSAPGAQFGAKPEASSHAPTPPPQPHPSYQAPAPPPALSVFQHSPQGAPITAPPPASSHHEHIAAPQARFTPTPGAPSPWHATPAELKFQTPWNTTPQYHAPPGAQAAAPEAPAAPAPGAPAARSNVDLLSDLLGDFNLPPQPIQPTVHSTQQLQHQAPQQQEMSDFRIAAATLNPPPESVKPVKPAAAVQPMPQMTAQQVPAHGAQETPILKSSELSSSASTSSSITELSDPSKFQLGVGNVQKLEKRMLHQSFRNQGPPPPMNQSDPLNQIDAFSSFMTPRHQ

Target ORF information:

RefSeq Version	NM_001264705.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRO1 domain-containing protein (ego-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001264705.2

ORF Insert Sequence:

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GTTTCAGACT 
CCATGGAACA CTACACCACA GTATCATGCT CCGCCAGGAG CTCAAGCTGC TGCTCCAGAA 
GCCCCCGCGG CTCCGGCTCC GGGAGCCCCA GCTGCTCGGA GCAACGTGGA TTTGCTGAGC 
GACCTGCTCG GCGACTTTAA CTTGCCTCCA CAGCCGATTC AGCCGACGGT TCACAGCACT 
CAACAGCTCC AGCATCAGGC TCCCCAGCAG CAGGAGATGT CGGATTTTCG AATTGCCGCC 
GCCACACTGA ATCCGCCGCC GGAATCGGTT AAACCGGTGA AACCGGCAGC CGCTGTTCAG 
CCAATGCCTC AAATGACGGC TCAACAAGTA CCGGCTCACG GAGCACAAGA GACTCCGATC 
CTCAAATCGT CTGAGCTTTC GAGCTCGGCG AGCACAAGCA GCAGCATCAC AGAGCTCTCG 
GATCCGAGCA AATTCCAGTT GGGAGTTGGA AATGTGCAGA AGCTGGAAAA ACGAATGCTT 
CATCAGAGTT TTAGAAACCA AGGACCGCCT CCACCAATGA ATCAATCGGA TCCACTCAAT 
CAAATTGATG CATTCAGTAG CTTCATGACG CCACGTCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ego-2 ( NM_001083153.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076622.2 Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.

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CloneID	OCa335486
Clone ID Related Accession (Same CDS sequence)	NM_001083153.3 , NM_001083153.4
Accession Version	NM_001083153.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Enhancer of Glp-One (glp-1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001083153.2. COMPLETENESS: incomplete on both ends.

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GACTCCATGG 
AACACTACAC CACAGTATCA TGCTCCGCCA GGAGCTCAAG CTGCTGCTCC AGAAGCCCCC 
GCGGCTCCGG CTCCGGGAGC CCCAGCTGCT CGGAGCAACG TGGATTTGCT GAGCGACCTG 
CTCGGCGACT TTAACTTGCC TCCACAGCCG ATTCAGCCGA CGGTTCACAG CACTCAACAG 
CTCCAGCATC AGGCTCCCCA GCAGCAGGAG ATGTCGGATT TTCGAATTGC CGCCGCCACA 
CTGAATCCGC CGCCGGAATC GGTTAAACCG GTGAAACCGG CAGCCGCTGT TCAGCCAATG 
CCTCAAATGA CGGCTCAACA AGTACCGGCT CACGGAGCAC AAGAGACTCC GATCCTCAAA 
TCGTCTGAGC TTTCGAGCTC GGCGAGCACA AGCAGCAGCA TCACAGAGCT CTCGGATCCG 
AGCAAATTCC AGTTGGGAGT TGGAAATGTG CAGAAGCTGG AAAAACGAAT GCTTCATCAG 
AGTTTTAGAA ACCAAGGACC GCCTCCACCA ATGAATCAAT CGGATCCACT CAATCAAATT 
GATGCATTCA GTAGCTTCAT GACGCCACGT CATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076622.2
CDS	1..4479
Translation	MEALPIMPMLAPQSRSSTLSYDNIPNFDFRLRMKEYILLTFNADPHDYDSAFDELTQMKFEANVPTPSVEQTLKLKRYYGQLCMMQKRFPMGAGEQLETPFAWHDGLIDIRSAQSEVTICDIEFEKASVMFNIGACHAQYAAEQTRDTQDCIKAAFMHFQYAAYAFEQLNSFRNSDIFYPSVDLDANVISFYYKVMIAQAQECLVQKSLLDNRSAIVIAKLSLWLQEAYDSAAKIVDEWSVNIPESVQRYYAKICSLKSAMYAVIAYMSFGDNLEKEDKKMGWRLQYYNIANKYMELLSSNHSKMRERYPELFVTSSFLFDVISAKQRNAEKENDFIYHDRVPKQEDAIDAVQKDGGGAMCKVKTLSFDPLDPSVCGCDLFGKLLPTFVQDAVKKYSDDKDQALREIKECVRSYDEHLNYQLQLAEFDKLRFMLNEGKRSREAWFEISEDLMKRNADMTSYPDCVPNLIDKMGESSDTARVAEAKLNTLLSKLRAIDLQKSSIRSDEGFILIQKELERLAEHLEQAKAHNVSLNKAIAQHSANLQLLTLPCREMWMKIVPPEQQGEMRNGSSPEELQVRKMIEKVMEMQAQRRKLVEQFEADLKADNISNKLMGTNERGAEEIMKSELTKHTNIQQLIRLNITAQDAILRAFADANADFFEERLAMSTKKEEYEKRVSELCASYEVYRDVSKKVDEGEQFYRQLMARCDQFAIPVHAMEEQYREEMEKKERAKKEAEHHLNQIRMSRDAQNALMDFGGGRGGGAPGARAQQQAYQPSRGAAGAGAGAPRGPRLGDFMDSYRARKQAPQAQIPEDQGPPSPTPSSICDFPVQSQRSQRFGAFEGAQGAPGAQRGYQGPHSPVPGALTGSQASGPFGAPPGAPHGYGAPLQAPPQAPYGYDPSRYQDPSTYPAPPPILPGSAAYEHAIRPYPGDTGSYQVPRIVPGAQYGAQAGSGAYFGAQFGAQGAQGAHFGAQGVQFGAQGAQGAHFGAQGAQGAQFGAQGAQGEQFGAQGAQGAHFGAQGAQGAHFGAQGAQGTQFGAQGAQGPPPASYGAPTPPQASYGPAPGAHGYQNGAQGPPGAEVGAQGPPGAHFGAHGASAPPPTSYGAPTPQRPPQASYGAAPGAQGPPGGQFEAHGAAALPPTSHGAPTPQGPFGAAPGAQFGAQGPYGQQGARYEAQKSPGAAIFGAPGAPPQHQGSFGAQFGVPPPQNSAPGAQFGAKPEASSHAPTPPPQPHPSYQAPAPPPALSVFQHSPQGAPITAPPPASSHHEHIAAPQARFTPTPGAPSPWHATPAELKTPWNTTPQYHAPPGAQAAAPEAPAAPAPGAPAARSNVDLLSDLLGDFNLPPQPIQPTVHSTQQLQHQAPQQQEMSDFRIAAATLNPPPESVKPVKPAAAVQPMPQMTAQQVPAHGAQETPILKSSELSSSASTSSSITELSDPSKFQLGVGNVQKLEKRMLHQSFRNQGPPPPMNQSDPLNQIDAFSSFMTPRHQ

Target ORF information:

RefSeq Version	NM_001083153.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083153.3

ORF Insert Sequence:

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GACTCCATGG 
AACACTACAC CACAGTATCA TGCTCCGCCA GGAGCTCAAG CTGCTGCTCC AGAAGCCCCC 
GCGGCTCCGG CTCCGGGAGC CCCAGCTGCT CGGAGCAACG TGGATTTGCT GAGCGACCTG 
CTCGGCGACT TTAACTTGCC TCCACAGCCG ATTCAGCCGA CGGTTCACAG CACTCAACAG 
CTCCAGCATC AGGCTCCCCA GCAGCAGGAG ATGTCGGATT TTCGAATTGC CGCCGCCACA 
CTGAATCCGC CGCCGGAATC GGTTAAACCG GTGAAACCGG CAGCCGCTGT TCAGCCAATG 
CCTCAAATGA CGGCTCAACA AGTACCGGCT CACGGAGCAC AAGAGACTCC GATCCTCAAA 
TCGTCTGAGC TTTCGAGCTC GGCGAGCACA AGCAGCAGCA TCACAGAGCT CTCGGATCCG 
AGCAAATTCC AGTTGGGAGT TGGAAATGTG CAGAAGCTGG AAAAACGAAT GCTTCATCAG 
AGTTTTAGAA ACCAAGGACC GCCTCCACCA ATGAATCAAT CGGATCCACT CAATCAAATT 
GATGCATTCA GTAGCTTCAT GACGCCACGT CATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ego-2 ( NM_001264705.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001251634.1 Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.

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CloneID	OCa336093
Clone ID Related Accession (Same CDS sequence)	NM_001264705.2 , NM_001264705.1
Accession Version	NM_001264705.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Enhancer of Glp-One (glp-1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GTTTCAGACT 
CCATGGAACA CTACACCACA GTATCATGCT CCGCCAGGAG CTCAAGCTGC TGCTCCAGAA 
GCCCCCGCGG CTCCGGCTCC GGGAGCCCCA GCTGCTCGGA GCAACGTGGA TTTGCTGAGC 
GACCTGCTCG GCGACTTTAA CTTGCCTCCA CAGCCGATTC AGCCGACGGT TCACAGCACT 
CAACAGCTCC AGCATCAGGC TCCCCAGCAG CAGGAGATGT CGGATTTTCG AATTGCCGCC 
GCCACACTGA ATCCGCCGCC GGAATCGGTT AAACCGGTGA AACCGGCAGC CGCTGTTCAG 
CCAATGCCTC AAATGACGGC TCAACAAGTA CCGGCTCACG GAGCACAAGA GACTCCGATC 
CTCAAATCGT CTGAGCTTTC GAGCTCGGCG AGCACAAGCA GCAGCATCAC AGAGCTCTCG 
GATCCGAGCA AATTCCAGTT GGGAGTTGGA AATGTGCAGA AGCTGGAAAA ACGAATGCTT 
CATCAGAGTT TTAGAAACCA AGGACCGCCT CCACCAATGA ATCAATCGGA TCCACTCAAT 
CAAATTGATG CATTCAGTAG CTTCATGACG CCACGTCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001251634.1
CDS	1..4485
Translation	MEALPIMPMLAPQSRSSTLSYDNIPNFDFRLRMKEYILLTFNADPHDYDSAFDELTQMKFEANVPTPSVEQTLKLKRYYGQLCMMQKRFPMGAGEQLETPFAWHDGLIDIRSAQSEVTICDIEFEKASVMFNIGACHAQYAAEQTRDTQDCIKAAFMHFQYAAYAFEQLNSFRNSDIFYPSVDLDANVISFYYKVMIAQAQECLVQKSLLDNRSAIVIAKLSLWLQEAYDSAAKIVDEWSVNIPESVQRYYAKICSLKSAMYAVIAYMSFGDNLEKEDKKMGWRLQYYNIANKYMELLSSNHSKMRERYPELFVTSSFLFDVISAKQRNAEKENDFIYHDRVPKQEDAIDAVQKDGGGAMCKVKTLSFDPLDPSVCGCDLFGKLLPTFVQDAVKKYSDDKDQALREIKECVRSYDEHLNYQLQLAEFDKLRFMLNEGKRSREAWFEISEDLMKRNADMTSYPDCVPNLIDKMGESSDTARVAEAKLNTLLSKLRAIDLQKSSIRSDEGFILIQKELERLAEHLEQAKAHNVSLNKAIAQHSANLQLLTLPCREMWMKIVPPEQQGEMRNGSSPEELQVRKMIEKVMEMQAQRRKLVEQFEADLKADNISNKLMGTNERGAEEIMKSELTKHTNIQQLIRLNITAQDAILRAFADANADFFEERLAMSTKKEEYEKRVSELCASYEVYRDVSKKVDEGEQFYRQLMARCDQFAIPVHAMEEQYREEMEKKERAKKEAEHHLNQIRMSRDAQNALMDFGGGRGGGAPGARAQQQAYQPSRGAAGAGAGAPRGPRLGDFMDSYRARKQAPQAQIPEDQGPPSPTPSSICDFPVQSQRSQRFGAFEGAQGAPGAQRGYQGPHSPVPGALTGSQASGPFGAPPGAPHGYGAPLQAPPQAPYGYDPSRYQDPSTYPAPPPILPGSAAYEHAIRPYPGDTGSYQVPRIVPGAQYGAQAGSGAYFGAQFGAQGAQGAHFGAQGVQFGAQGAQGAHFGAQGAQGAQFGAQGAQGEQFGAQGAQGAHFGAQGAQGAHFGAQGAQGTQFGAQGAQGPPPASYGAPTPPQASYGPAPGAHGYQNGAQGPPGAEVGAQGPPGAHFGAHGASAPPPTSYGAPTPQRPPQASYGAAPGAQGPPGGQFEAHGAAALPPTSHGAPTPQGPFGAAPGAQFGAQGPYGQQGARYEAQKSPGAAIFGAPGAPPQHQGSFGAQFGVPPPQNSAPGAQFGAKPEASSHAPTPPPQPHPSYQAPAPPPALSVFQHSPQGAPITAPPPASSHHEHIAAPQARFTPTPGAPSPWHATPAELKFQTPWNTTPQYHAPPGAQAAAPEAPAAPAPGAPAARSNVDLLSDLLGDFNLPPQPIQPTVHSTQQLQHQAPQQQEMSDFRIAAATLNPPPESVKPVKPAAAVQPMPQMTAQQVPAHGAQETPILKSSELSSSASTSSSITELSDPSKFQLGVGNVQKLEKRMLHQSFRNQGPPPPMNQSDPLNQIDAFSSFMTPRHQ

Target ORF information:

RefSeq Version	NM_001264705.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Enhancer of Glp-One (glp-1) (ego-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001264705.1

ORF Insert Sequence:

ATGGAGGCTC TTCCAATAAT GCCTATGCTG GCTCCACAAT CGAGATCCTC AACACTTTCC 
TACGATAATA TCCCAAATTT TGACTTTAGA TTACGCATGA AAGAGTATAT TCTGCTCACT 
TTCAACGCGG ACCCCCACGA CTACGATTCG GCGTTCGACG AGCTGACACA AATGAAATTT 
GAAGCCAACG TTCCGACGCC ATCTGTGGAG CAAACTCTGA AATTAAAAAG ATATTACGGC 
CAGCTGTGTA TGATGCAGAA AAGATTCCCA ATGGGAGCCG GAGAACAGCT TGAAACACCG 
TTTGCATGGC ACGACGGTCT AATCGACATC AGAAGTGCTC AAAGTGAAGT GACAATTTGT 
GATATTGAAT TCGAAAAAGC GTCTGTAATG TTCAATATTG GAGCATGTCA TGCACAATAT 
GCTGCCGAGC AGACAAGGGA TACACAGGAT TGTATAAAAG CAGCATTTAT GCACTTTCAA 
TATGCTGCCT ACGCCTTCGA ACAGCTCAAT TCATTCAGAA ATTCGGATAT TTTTTATCCG 
TCAGTGGATT TGGATGCAAA TGTCATTTCG TTTTATTATA AAGTGATGAT TGCTCAAGCT 
CAAGAATGTC TGGTTCAAAA GTCACTTTTA GACAATCGAA GTGCAATTGT AATTGCAAAA 
TTATCACTTT GGTTACAGGA AGCTTATGAT TCAGCAGCGA AAATTGTCGA TGAATGGAGT 
GTTAATATAC CTGAATCTGT ACAGAGGTAT TATGCGAAGA TATGCTCATT AAAAAGTGCA 
ATGTATGCTG TAATTGCCTA TATGTCATTT GGTGATAATT TGGAAAAAGA AGACAAGAAA 
ATGGGTTGGA GGCTTCAATA TTATAATATT GCCAACAAAT ATATGGAATT ACTCTCATCG 
AATCATTCGA AAATGCGGGA ACGATATCCG GAGCTTTTCG TCACTTCAAG CTTCCTTTTT 
GATGTGATTA GTGCCAAGCA AAGAAACGCG GAAAAAGAGA ACGATTTCAT CTATCACGAC 
AGAGTTCCAA AACAAGAAGA TGCAATAGAT GCTGTACAGA AAGACGGTGG TGGTGCAATG 
TGTAAAGTGA AAACTCTGTC ATTTGATCCT CTAGATCCAT CAGTTTGTGG TTGTGATCTA 
TTCGGGAAGC TTCTACCCAC ATTTGTACAG GATGCAGTGA AAAAGTATTC AGATGACAAG 
GATCAAGCAC TTCGAGAAAT CAAAGAATGT GTCAGATCCT ATGATGAACA TCTCAATTAT 
CAACTACAAC TCGCCGAATT TGATAAACTT CGATTTATGC TCAATGAGGG AAAGAGGAGC 
CGAGAAGCTT GGTTCGAGAT TTCTGAAGAT TTGATGAAAA GGAATGCTGA CATGACGTCA 
TACCCGGATT GTGTACCAAA TTTAATCGAT AAAATGGGAG AATCATCGGA TACAGCGAGA 
GTTGCTGAAG CGAAGCTTAA TACGTTGCTC TCAAAGCTTC GAGCTATTGA TTTGCAGAAG 
AGCAGCATTC GGTCGGATGA AGGCTTTATC CTCATTCAGA AGGAGCTCGA ACGGCTCGCC 
GAGCATCTGG AGCAGGCAAA AGCTCATAAT GTTTCACTGA ATAAGGCGAT TGCACAGCAC 
TCTGCGAATT TGCAACTTCT CACGCTTCCG TGTAGAGAGA TGTGGATGAA AATTGTGCCG 
CCGGAGCAGC AGGGAGAGAT GAGAAATGGC TCGAGTCCGG AAGAGCTCCA AGTGCGAAAA 
ATGATTGAAA AAGTGATGGA AATGCAGGCG CAACGTCGAA AGCTCGTCGA ACAATTTGAA 
GCCGATTTGA AAGCGGATAA TATCTCCAAT AAGCTTATGG GAACCAATGA ACGGGGAGCC 
GAGGAAATCA TGAAATCGGA GCTCACAAAG CACACGAACA TCCAACAGCT CATTCGGCTT 
AACATCACAG CTCAAGACGC AATTCTTCGT GCATTTGCAG ATGCAAACGC TGATTTCTTC 
GAAGAACGTT TGGCGATGAG CACAAAAAAA GAGGAATACG AGAAGCGTGT CTCGGAGCTC 
TGTGCTTCTT ATGAAGTTTA TCGAGATGTT TCCAAAAAAG TTGATGAGGG AGAACAATTC 
TATCGGCAGT TGATGGCTAG ATGTGATCAA TTTGCGATTC CGGTTCATGC GATGGAAGAG 
CAATATCGCG AAGAGATGGA AAAGAAGGAA AGAGCCAAGA AAGAGGCGGA GCATCATTTG 
AATCAAATTC GAATGAGCCG AGATGCTCAA AATGCTCTTA TGGATTTTGG TGGAGGACGA 
GGAGGAGGAG CTCCCGGAGC ACGAGCACAA CAACAAGCCT ATCAACCATC CAGAGGAGCA 
GCTGGAGCTG GAGCTGGAGC TCCACGAGGA CCACGGCTCG GAGACTTTAT GGATAGCTAC 
CGGGCTCGGA AACAGGCCCC GCAGGCTCAA ATTCCAGAGG ATCAAGGACC ACCATCGCCC 
ACGCCGAGCA GTATTTGTGA TTTTCCTGTG CAGAGCCAGC GAAGTCAGAG ATTTGGAGCA 
TTTGAGGGGG CTCAGGGAGC TCCGGGAGCC CAGAGAGGAT ACCAGGGACC TCACAGCCCG 
GTTCCGGGAG CTTTAACAGG CTCACAGGCT TCTGGGCCTT TTGGAGCACC GCCAGGAGCC 
CCACACGGCT ATGGAGCACC ACTTCAAGCT CCACCACAAG CTCCATATGG CTATGATCCA 
TCCCGTTACC AAGATCCATC GACATATCCG GCTCCACCGC CGATTCTTCC GGGCTCAGCG 
GCCTATGAGC ATGCGATTAG GCCGTATCCG GGGGATACGG GCTCGTATCA GGTGCCACGA 
ATCGTCCCCG GAGCACAATA TGGAGCTCAA GCCGGCTCTG GAGCATACTT TGGAGCACAA 
TTCGGAGCTC AGGGAGCCCA AGGAGCACAT TTTGGAGCAC AAGGAGTACA ATTTGGAGCT 
CAGGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAGCACA ATTCGGAGCT 
CAGGGAGCCC AAGGAGAACA ATTTGGAGCT CAGGGAGCCC AAGGAGCACA TTTTGGAGCA 
CAAGGAGCCC AAGGAGCACA TTTTGGAGCA CAAGGAGCCC AAGGAACACA ATTTGGAGCT 
CAGGGAGCCC AAGGTCCACC ACCGGCAAGC TATGGAGCCC CCACACCACC TCAAGCTTCC 
TATGGACCTG CTCCAGGAGC ACATGGATAT CAAAACGGAG CCCAGGGACC CCCTGGAGCT 
GAAGTCGGAG CCCAAGGTCC TCCTGGAGCA CATTTTGGAG CTCATGGAGC TTCAGCACCA 
CCACCAACAA GCTATGGAGC CCCCACACCA CAAAGACCAC CTCAAGCTTC CTATGGAGCT 
GCTCCGGGAG CACAAGGACC CCCTGGAGGA CAATTTGAAG CTCATGGAGC TGCAGCACTA 
CCACCAACAA GCCACGGAGC CCCCACACCT CAAGGACCGT TTGGAGCTGC TCCAGGAGCA 
CAATTTGGAG CACAAGGACC GTACGGACAG CAAGGAGCCC GCTATGAAGC TCAAAAATCG 
CCAGGAGCAG CAATTTTTGG AGCTCCGGGA GCACCACCAC AACATCAAGG ATCCTTTGGA 
GCACAGTTCG GAGTACCACC GCCTCAAAAT TCAGCTCCTG GAGCTCAATT TGGAGCCAAG 
CCAGAAGCTT CATCTCATGC TCCAACCCCA CCACCACAAC CTCACCCATC ATACCAGGCA 
CCGGCTCCAC CACCGGCTCT TTCCGTCTTC CAGCATTCTC CACAAGGAGC ACCGATAACA 
GCTCCACCGC CAGCTTCTTC TCATCATGAG CATATTGCTG CTCCTCAAGC TCGTTTCACA 
CCGACTCCAG GTGCTCCGTC GCCATGGCAT GCGACTCCGG CTGAGCTCAA GTTTCAGACT 
CCATGGAACA CTACACCACA GTATCATGCT CCGCCAGGAG CTCAAGCTGC TGCTCCAGAA 
GCCCCCGCGG CTCCGGCTCC GGGAGCCCCA GCTGCTCGGA GCAACGTGGA TTTGCTGAGC 
GACCTGCTCG GCGACTTTAA CTTGCCTCCA CAGCCGATTC AGCCGACGGT TCACAGCACT 
CAACAGCTCC AGCATCAGGC TCCCCAGCAG CAGGAGATGT CGGATTTTCG AATTGCCGCC 
GCCACACTGA ATCCGCCGCC GGAATCGGTT AAACCGGTGA AACCGGCAGC CGCTGTTCAG 
CCAATGCCTC AAATGACGGC TCAACAAGTA CCGGCTCACG GAGCACAAGA GACTCCGATC 
CTCAAATCGT CTGAGCTTTC GAGCTCGGCG AGCACAAGCA GCAGCATCAC AGAGCTCTCG 
GATCCGAGCA AATTCCAGTT GGGAGTTGGA AATGTGCAGA AGCTGGAAAA ACGAATGCTT 
CATCAGAGTT TTAGAAACCA AGGACCGCCT CCACCAATGA ATCAATCGGA TCCACTCAAT 
CAAATTGATG CATTCAGTAG CTTCATGACG CCACGTCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ego-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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