vwa-8 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	vwa-8
Entrez Gene ID	181431
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_077759.4	NP_510160.2	Von (von) Willebrand factor A domain containing
NM_077760.4	NP_510161.2	Von (von) Willebrand factor A domain containing
NM_001375159.1	NP_001362055.1	VWFA domain-containing protein
NM_001270282.1	NP_001257211.1	Von (von) Willebrand factor A domain containing
NM_001307851.1	NP_001294780.1	Von (von) Willebrand factor A domain containing
NM_001129655.2	NP_001123127.1	Von (von) Willebrand factor A domain containing
NM_077759.6	NP_510160.2	VWFA domain-containing protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	VWA8	NP_055873.1
Mus musculus (house mouse)	Vwa8	NP_082182.1
	VWA8	XP_001091849.2
Bos taurus (cattle)	VWA8	XP_002691869.1
Canis lupus familiaris (dog)	VWA8	XP_005633992.1
Gallus gallus (chicken)	KIAA0564	XP_425624.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000545	XP_310539.4
Caenorhabditis elegans (roundworm)	F11C1.5	NP_001257211.1
Pan troglodytes (chimpanzee)	VWA8	XP_003318070.1
Danio rerio (zebrafish)	vwa8	NP_001121810.1
Drosophila melanogaster (fruit fly)	c12.2	NP_652604.2
Xenopus tropicalis (tropical clawed frog)	vwa8	NP_001096540.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vwa-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vwa-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa355672	NM_001129655.2 Latest version!	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa355674	NM_001307851.1 Latest version!	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa355674	NM_001375159.1 Latest version!	Caenorhabditis elegans VWFA domain-containing protein (vwa-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa355673	NM_077760.4 Latest version!	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa355670	NM_001270282.1 Latest version!	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa355671	NM_077759.6 Latest version!	Caenorhabditis elegans VWFA domain-containing protein (vwa-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa355671	NM_077759.4	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vwa-8 ( NM_001129655.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001123127.1 Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Price & Availability↑

CloneID	OCa355672
Clone ID Related Accession (Same CDS sequence)	NM_001129655.2
Accession Version	NM_001129655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-11
Organism	Caenorhabditis elegans
Product	Von (von) Willebrand factor A domain containing
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001129655.1. COMPLETENESS: incomplete on both ends.

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGATA TGACGCCGAT 
GCCTATGAAA AAGTGTGGAA CAAAGTTCAA GCTCCATCAA GAAAACTAGC ATCTGTGATT 
GACCAATTGG AGGCAAAGAA AAAAGAAAGA GAATGGACAA AGCATCAAAC TAGCGGAGAT 
TTGGATGACG GAAAATTGAT TGAAGGAGTC ACCGGAGAGC AGAATATTTA CAGGCGACGC 
ATCGACAAAG TTCCAGATCC GGGAGCTCCA CAAACAAAGC CAAAACGCCT GAAAGTATGC 
TTGGATGTTT CTGGTTCCAT GTACAGATTT AATGGATATG ACCAGAGACT CGTGAAGTCA 
CTTGAAGCAG CCTTGATGAC AATGACTGCA CTTGATGGAA AAACGGACAA AGTGCAGTAC 
GACATTATTG GACATTCTGG AGACTCTCCA TGCGTGTCAT TTGTAAAGAC CGATCATCAT 
CCCAAAAACA ACAAAGAACG TCTGGATACA TTGAAACGCA TGATTGCGCA TACGCAGTAC 
TGCGTCAGTG GTGACAATAC GGTAGAGAGT TTACAATTTG CAATCAAAGA ATTGGCCGCA 
AAGAAAGATG ATTTTGATGA AACCGTTGTC ATTTTGGTGT CAGATGCTAA TTTGGAAAGG 
TATGGAATTC AACCAAAGGA ATTGAAAGAT GCAATGGCTA AAGAGCCAAA TATCAACTCA 
TTTGTCATTT TCATTGGGTC GCTCAGTGAC GAAGCCGACC AGCTACAACG AGAGTTGCCT 
GTTGGAAAAG CTTTTGTTTT AAAAGACACT TCTGAGCTTC CAAAAATTAT GGAGACCATT 
TTCTCATCAA CTATTGCTCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123127.1
CDS	1..2364
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGYDADAYEKVWNKVQAPSRKLASVIDQLEAKKKEREWTKHQTSGDLDDGKLIEGVTGEQNIYRRRIDKVPDPGAPQTKPKRLKVCLDVSGSMYRFNGYDQRLVKSLEAALMTMTALDGKTDKVQYDIIGHSGDSPCVSFVKTDHHPKNNKERLDTLKRMIAHTQYCVSGDNTVESLQFAIKELAAKKDDFDETVVILVSDANLERYGIQPKELKDAMAKEPNINSFVIFIGSLSDEADQLQRELPVGKAFVLKDTSELPKIMETIFSSTIAQ

Target ORF information:

RefSeq Version	NM_001129655.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129655.2

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGATA TGACGCCGAT 
GCCTATGAAA AAGTGTGGAA CAAAGTTCAA GCTCCATCAA GAAAACTAGC ATCTGTGATT 
GACCAATTGG AGGCAAAGAA AAAAGAAAGA GAATGGACAA AGCATCAAAC TAGCGGAGAT 
TTGGATGACG GAAAATTGAT TGAAGGAGTC ACCGGAGAGC AGAATATTTA CAGGCGACGC 
ATCGACAAAG TTCCAGATCC GGGAGCTCCA CAAACAAAGC CAAAACGCCT GAAAGTATGC 
TTGGATGTTT CTGGTTCCAT GTACAGATTT AATGGATATG ACCAGAGACT CGTGAAGTCA 
CTTGAAGCAG CCTTGATGAC AATGACTGCA CTTGATGGAA AAACGGACAA AGTGCAGTAC 
GACATTATTG GACATTCTGG AGACTCTCCA TGCGTGTCAT TTGTAAAGAC CGATCATCAT 
CCCAAAAACA ACAAAGAACG TCTGGATACA TTGAAACGCA TGATTGCGCA TACGCAGTAC 
TGCGTCAGTG GTGACAATAC GGTAGAGAGT TTACAATTTG CAATCAAAGA ATTGGCCGCA 
AAGAAAGATG ATTTTGATGA AACCGTTGTC ATTTTGGTGT CAGATGCTAA TTTGGAAAGG 
TATGGAATTC AACCAAAGGA ATTGAAAGAT GCAATGGCTA AAGAGCCAAA TATCAACTCA 
TTTGTCATTT TCATTGGGTC GCTCAGTGAC GAAGCCGACC AGCTACAACG AGAGTTGCCT 
GTTGGAAAAG CTTTTGTTTT AAAAGACACT TCTGAGCTTC CAAAAATTAT GGAGACCATT 
TTCTCATCAA CTATTGCTCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa-8 ( NM_001375159.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001362055.1 Caenorhabditis elegans VWFA domain-containing protein (vwa-8), mRNA.

Price & Availability↑

CloneID	OCa355674
Clone ID Related Accession (Same CDS sequence)	NM_001375159.1 , NM_001307851.1
Accession Version	NM_001375159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	VWFA domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284).

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362055.1
CDS	24..2948
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGVDEATMNERGVFQIPDSFRVLFVGESQSKEHKWINENVLSILPFYTVPNLSLSEQMSIISEITHGSNTESVDKLIKFVQTIKNSNDAGLKNTATSLSLRRLIHIAKRDLMQPGHLRELVEKAALSKFLPQITKETFEHELNTANVFNDTTIRTKENEKFLDMIRANTIKAEDEALIPNVLFHHNKQHDEILEDMARDMKLGSHLLLIGNQGVGKNKLTDRFLHLINRPRQYMQLHRDTTVQTLTMQTVVENGIIRHEDSALVKAARSGQILVIDEADKAPLHVIAILKSLLDTGTLVLGDGRTLRPASSFTEADKTNDRIVPIHPNFRIIMLANRPGFPFLGNNLFAVLGDLFAIHMIDHPGRDSELAMIKKFGPTVPVKTLNQLLTIFNELREKTDQGLLQYPYSTRELVNIVRHCNEFPTDPLPEVCRNVFDFDSYSEDTIATIHEVFQKHGVPLG

Target ORF information:

RefSeq Version	NM_001375159.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans VWFA domain-containing protein (vwa-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375159.1

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa-8 ( NM_001307851.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294780.1 Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

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CloneID	OCa355674
Clone ID Related Accession (Same CDS sequence)	NM_001375159.1 , NM_001307851.1
Accession Version	NM_001307851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Von (von) Willebrand factor A domain containing
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294780.1
CDS	1..2925
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGVDEATMNERGVFQIPDSFRVLFVGESQSKEHKWINENVLSILPFYTVPNLSLSEQMSIISEITHGSNTESVDKLIKFVQTIKNSNDAGLKNTATSLSLRRLIHIAKRDLMQPGHLRELVEKAALSKFLPQITKETFEHELNTANVFNDTTIRTKENEKFLDMIRANTIKAEDEALIPNVLFHHNKQHDEILEDMARDMKLGSHLLLIGNQGVGKNKLTDRFLHLINRPRQYMQLHRDTTVQTLTMQTVVENGIIRHEDSALVKAARSGQILVIDEADKAPLHVIAILKSLLDTGTLVLGDGRTLRPASSFTEADKTNDRIVPIHPNFRIIMLANRPGFPFLGNNLFAVLGDLFAIHMIDHPGRDSELAMIKKFGPTVPVKTLNQLLTIFNELREKTDQGLLQYPYSTRELVNIVRHCNEFPTDPLPEVCRNVFDFDSYSEDTIATIHEVFQKHGVPLG

Target ORF information:

RefSeq Version	NM_001307851.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307851.1

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa-8 ( NM_077760.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510161.2 Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Price & Availability↑

CloneID	OCa355673
Clone ID Related Accession (Same CDS sequence)	NM_077760.4
Accession Version	NM_077760.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-11
Organism	Caenorhabditis elegans
Product	Von (von) Willebrand factor A domain containing
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_077760.3. COMPLETENESS: incomplete on both ends.

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGGTTGACG AAATCAACAT ACACAAGATT 
ATCCCTCGTT TTCGTGCAAT TAACAAACCA CGAATTAGAC TAGCATATCT TGGTGGAGAC 
AATGTGCTGC TACACGAAGA AGAGGCGAAC TTCACAGCGA TCATCAGCTT GCCCGACAAA 
ATGGCGGCTG TTGTTACAAA GCAAACGAGT TTGGGTGAAA CGCTTGGAGC TATGTTTACC 
GGTTCCAATT ATTCATGGAG ATTATCAAAT CAACAAAGCA ATCACGCTTT GCTTTATGAG 
TGTGGAGGGC GCGAAATGCT GGTGTATACC GATGAAAAAC ACGGAGTCAC TGTCCAGAAC 
GGCACGGTAC CGTTTAACAT CAAACGAGTA TTTGCTTCAG CTCCTCAACA ATGGACTGTA 
GTTTCAATGG AAAATGAGAA CTATCTTCTG AAGGTGGAAC TCGGAAATTT TACTTTGGAG 
AAAATTGACA ACGATTTGGA AGGCGGCCTA GATACGGTTG CAACAAAGTT GGACAAGAAT 
GCAATGACTT TAGTTGCCGA GCCATACTAC TTTGTTCAAG GAAACCATTC TGGAAGTGTT 
TCCGGCGTAC TCCGAAAGAA ATCTGAAAAT ACGTTTCATA AACGGCCGTA TAGCTTTACT 
GACGATAGAA TTGCTGCAAA TGATGTGAGC AGGGAAAATG CATTTTTGGG CAGTACATTG 
GTTCGTGCAG TACCTGGGTT CATGACTCCA ACGGAATGTG TCGAAGAGAA GACTGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510161.2
CDS	1..4020
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGVDEATMNERGVFQIPDSFRVLFVGESQSKEHKWINENVLSILPFYTVPNLSLSEQMSIISEITHGSNTESVDKLIKFVQTIKNSNDAGLKNTATSLSLRRLIHIAKRDLMQPGHLRELVEKAALSKFLPQITKETFEHELNTANVFNDTTIRTKENEKFLDMIRANTIKAEDEALIPNVLFHHNKQHDEILEDMARDMKLGSHLLLIGNQGVGKNKLTDRFLHLINRPRQYMQLHRDTTVQTLTMQTVVENGIIRHEDSALVKAARSGQILVIDEADKAPLHVIAILKSLLDTGTLVLGDGRTLRPASSFTEADKTNDRIVPIHPNFRIIMLANRPGFPFLGNNLFAVLGDLFAIHMIDHPGRDSELAMIKKFGPTVPVKTLNQLLTIFNELREKTDQGLLQYPYSTRELVNIVRHCNEFPTDPLPEVCRNVFDFDSYSEDTIATIHEVFQKHGVPLGVIRTGESLSIMERIPLGEMKTIGKWSRQKVEFPKSYGFKTPVAVEIERPEKMEFGLRKLDKQSNRNNVFSELHCVWKLPTDESNIISDGIRINDDLLVASVNPPRLYHCKDFMDTESVDEINIHKIIPRFRAINKPRIRLAYLGGDNVLLHEEEANFTAIISLPDKMAAVVTKQTSLGETLGAMFTGSNYSWRLSNQQSNHALLYECGGREMLVYTDEKHGVTVQNGTVPFNIKRVFASAPQQWTVVSMENENYLLKVELGNFTLEKIDNDLEGGLDTVATKLDKNAMTLVAEPYYFVQGNHSGSVSGVLRKKSENTFHKRPYSFTDDRIAANDVSRENAFLGSTLVRAVPGFMTPTECVEEKTG

Target ORF information:

RefSeq Version	NM_077760.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077760.4

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGGTTGACG AAATCAACAT ACACAAGATT 
ATCCCTCGTT TTCGTGCAAT TAACAAACCA CGAATTAGAC TAGCATATCT TGGTGGAGAC 
AATGTGCTGC TACACGAAGA AGAGGCGAAC TTCACAGCGA TCATCAGCTT GCCCGACAAA 
ATGGCGGCTG TTGTTACAAA GCAAACGAGT TTGGGTGAAA CGCTTGGAGC TATGTTTACC 
GGTTCCAATT ATTCATGGAG ATTATCAAAT CAACAAAGCA ATCACGCTTT GCTTTATGAG 
TGTGGAGGGC GCGAAATGCT GGTGTATACC GATGAAAAAC ACGGAGTCAC TGTCCAGAAC 
GGCACGGTAC CGTTTAACAT CAAACGAGTA TTTGCTTCAG CTCCTCAACA ATGGACTGTA 
GTTTCAATGG AAAATGAGAA CTATCTTCTG AAGGTGGAAC TCGGAAATTT TACTTTGGAG 
AAAATTGACA ACGATTTGGA AGGCGGCCTA GATACGGTTG CAACAAAGTT GGACAAGAAT 
GCAATGACTT TAGTTGCCGA GCCATACTAC TTTGTTCAAG GAAACCATTC TGGAAGTGTT 
TCCGGCGTAC TCCGAAAGAA ATCTGAAAAT ACGTTTCATA AACGGCCGTA TAGCTTTACT 
GACGATAGAA TTGCTGCAAA TGATGTGAGC AGGGAAAATG CATTTTTGGG CAGTACATTG 
GTTCGTGCAG TACCTGGGTT CATGACTCCA ACGGAATGTG TCGAAGAGAA GACTGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa-8 ( NM_001270282.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001257211.1 Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

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CloneID	OCa355670
Clone ID Related Accession (Same CDS sequence)	NM_001270282.1
Accession Version	NM_001270282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-11
Organism	Caenorhabditis elegans
Product	Von (von) Willebrand factor A domain containing
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGAACAAGA TTTATTTTTT CCGAACCCAC 
ATTTCCGATT TTCAGGTTGA CGAAATCAAC ATACACAAGA TTATCCCTCG TTTTCGTGCA 
ATTAACAAAC CACGAATTAG ACTAGCATAT CTTGGTGGAG ACAATGTGCT GCTACACGAA 
GAAGAGGCGA ACTTCACAGC GATCATCAGC TTGCCCGACA AAATGGCGGC TGTTGTTACA 
AAGCAAACGA GTTTGGGTGA AACGCTTGGA GCTATGTTTA CCGGTTCCAA TTATTCATGG 
AGATTATCAA ATCAACAAAG CAATCACGCT TTGCTTTATG AGTGTGGAGG GCGCGAAATG 
CTGGTGTATA CCGATGAAAA ACACGGAGTC ACTGTCCAGA ACGGCACGGT ACCGTTTAAC 
ATCAAACGAG TATTTGCTTC AGCTCCTCAA CAATGGACTG TAGTTTCAAT GGAAAATGAG 
AACTATCTTC TGAAGGTGGA ACTCGGAAAT TTTACTTTGG AGAAAATTGA CAACGATTTG 
GAAGGCGGCC TAGATACGGT TGCAACAAAG TTGGACAAGA ATGCAATGAC TTTAGTTGCC 
GAGCCATACT ACTTTGTTCA AGGAAACCAT TCTGGAAGTG TTTCCGGCGT ACTCCGAAAG 
AAATCTGAAA ATACGTTTCA TAAACGGCCG TATAGCTTTA CTGACGATAG AATTGCTGCA 
AATGATGTGA GCAGGGAAAA TGCATTTTTG GGCAGTACAT TGGTTCGTGC AGTACCTGGG 
TTCATGACTC CAACGGAATG TGTCGAAGAG AAAATAAGGC CATTCGATTG TATTGGATTC 
CTCGAGACAA TCGATACGGA AAGAAAGAAA ATCTGCTATG TACCGGTTCC GCATAGTGGG 
AATATCAGTT TATACCAAGA CTGGGTAGCT TCAATCACCA AATCCGCATT CCATATTGTT 
CCATGGGACA ATGATAAAGT TCTCACTGTT GACAGCTCTG GAAGCATTCG TTCTTTCGAA 
TTAAGAATGT CGACATTGGA AAAATCATTT GATGACTGGA AGCGGATGAC TGGAGGGGCG 
GATGATGAGA AGTTGAGAAT GGAGTTTGAT AGAAAACCAG ACGACGTAGA CTTTAATAAA 
CTTGATGAGC CAAAACTCGG AAAGATCGAC CCCAACAATG CACCTCATCA CGGTGGAAAT 
CAATGGATGG GAGGAACTGG TGGATACAAT ACTGCAGGTC TCGGAGGAAT TGGAGGGCCG 
TTCAGACTTG ACGCAGGTCA TGATGTCCAT CAAATGCCAG ATTTTGCTAA GCAACAAGTC 
CCGAAGCACA TATTGAAAAA AGCCCGGGAG ATTGCAAAAG TAGAGTATGC AAAGAAATTG 
AGGGAAATCA ACATGTCGGA GTATGACGCC GATGCCTATG AAAAAGTGTG GAACAAAGTT 
CAAGCTCCAT CAAGAAAACT AGCATCTGTG ATTGACCAAT TGGAGGCAAA GAAAAAAGAA 
AGAGAATGGA CAAAGCATCA AACTAGCGGA GATTTGGATG ACGGAAAATT GATTGAAGGA 
GTCACCGGAG AGCAGAATAT TTACAGGCGA CGCATCGACA AAGTTCCAGA TCCGGGAGCT 
CCACAAACAA AGCCAAAACG CCTGAAAGTA TGCTTGGATG TTTCTGGTTC CATGTACAGA 
TTTAATGGAT ATGACCAGAG ACTCGTGAAG TCACTTGAAG CAGCCTTGAT GACAATGACT 
GCACTTGATG GAAAAACGGA CAAAGTGCAG TACGACATTA TTGGACATTC TGGAGACTCT 
CCATGCGTGT CATTTGTAAA GACCGATCAT CATCCCAAAA ACAACAAAGA ACGTCTGGAT 
ACATTGAAAC GCATGATTGC GCATACGCAG TACTGCGTCA GTGGTGACAA TACGGTAGAG 
AGTTTACAAT TTGCAATCAA AGAATTGGCC GCAAAGAAAG ATGATTTTGA TGAAACCGTT 
GTCATTTTGG TGTCAGATGC TAATTTGGAA AGGTATGGAA TTCAACCAAA GGAATTGAAA 
GATGCAATGG CTAAAGAGCC AAATATCAAC TCATTTGTCA TTTTCATTGG GTCGCTCAGT 
GACGAAGCCG ACCAGCTACA ACGAGAGTTG CCTGTTGGAA AAGCTTTTGT TTTAAAAGAC 
ACTTCTGAGC TTCCAAAAAT TATGGAGACC ATTTTCTCAT CAACTATTGC TCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257211.1
CDS	1..5457
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGVDEATMNERGVFQIPDSFRVLFVGESQSKEHKWINENVLSILPFYTVPNLSLSEQMSIISEITHGSNTESVDKLIKFVQTIKNSNDAGLKNTATSLSLRRLIHIAKRDLMQPGHLRELVEKAALSKFLPQITKETFEHELNTANVFNDTTIRTKENEKFLDMIRANTIKAEDEALIPNVLFHHNKQHDEILEDMARDMKLGSHLLLIGNQGVGKNKLTDRFLHLINRPRQYMQLHRDTTVQTLTMQTVVENGIIRHEDSALVKAARSGQILVIDEADKAPLHVIAILKSLLDTGTLVLGDGRTLRPASSFTEADKTNDRIVPIHPNFRIIMLANRPGFPFLGNNLFAVLGDLFAIHMIDHPGRDSELAMIKKFGPTVPVKTLNQLLTIFNELREKTDQGLLQYPYSTRELVNIVRHCNEFPTDPLPEVCRNVFDFDSYSEDTIATIHEVFQKHGVPLGVIRTGESLSIMERIPLGEMKTIGKWSRQKVEFPKSYGFKTPVAVEIERPEKMEFGLRKLDKQSNRNNVFSELHCVWKLPTDESNIISDGIRINDDLLVASVNPPRLYHCKDFMDTESNKIYFFRTHISDFQVDEINIHKIIPRFRAINKPRIRLAYLGGDNVLLHEEEANFTAIISLPDKMAAVVTKQTSLGETLGAMFTGSNYSWRLSNQQSNHALLYECGGREMLVYTDEKHGVTVQNGTVPFNIKRVFASAPQQWTVVSMENENYLLKVELGNFTLEKIDNDLEGGLDTVATKLDKNAMTLVAEPYYFVQGNHSGSVSGVLRKKSENTFHKRPYSFTDDRIAANDVSRENAFLGSTLVRAVPGFMTPTECVEEKIRPFDCIGFLETIDTERKKICYVPVPHSGNISLYQDWVASITKSAFHIVPWDNDKVLTVDSSGSIRSFELRMSTLEKSFDDWKRMTGGADDEKLRMEFDRKPDDVDFNKLDEPKLGKIDPNNAPHHGGNQWMGGTGGYNTAGLGGIGGPFRLDAGHDVHQMPDFAKQQVPKHILKKAREIAKVEYAKKLREINMSEYDADAYEKVWNKVQAPSRKLASVIDQLEAKKKEREWTKHQTSGDLDDGKLIEGVTGEQNIYRRRIDKVPDPGAPQTKPKRLKVCLDVSGSMYRFNGYDQRLVKSLEAALMTMTALDGKTDKVQYDIIGHSGDSPCVSFVKTDHHPKNNKERLDTLKRMIAHTQYCVSGDNTVESLQFAIKELAAKKDDFDETVVILVSDANLERYGIQPKELKDAMAKEPNINSFVIFIGSLSDEADQLQRELPVGKAFVLKDTSELPKIMETIFSSTIAQ

Target ORF information:

RefSeq Version	NM_001270282.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270282.1

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGAACAAGA TTTATTTTTT CCGAACCCAC 
ATTTCCGATT TTCAGGTTGA CGAAATCAAC ATACACAAGA TTATCCCTCG TTTTCGTGCA 
ATTAACAAAC CACGAATTAG ACTAGCATAT CTTGGTGGAG ACAATGTGCT GCTACACGAA 
GAAGAGGCGA ACTTCACAGC GATCATCAGC TTGCCCGACA AAATGGCGGC TGTTGTTACA 
AAGCAAACGA GTTTGGGTGA AACGCTTGGA GCTATGTTTA CCGGTTCCAA TTATTCATGG 
AGATTATCAA ATCAACAAAG CAATCACGCT TTGCTTTATG AGTGTGGAGG GCGCGAAATG 
CTGGTGTATA CCGATGAAAA ACACGGAGTC ACTGTCCAGA ACGGCACGGT ACCGTTTAAC 
ATCAAACGAG TATTTGCTTC AGCTCCTCAA CAATGGACTG TAGTTTCAAT GGAAAATGAG 
AACTATCTTC TGAAGGTGGA ACTCGGAAAT TTTACTTTGG AGAAAATTGA CAACGATTTG 
GAAGGCGGCC TAGATACGGT TGCAACAAAG TTGGACAAGA ATGCAATGAC TTTAGTTGCC 
GAGCCATACT ACTTTGTTCA AGGAAACCAT TCTGGAAGTG TTTCCGGCGT ACTCCGAAAG 
AAATCTGAAA ATACGTTTCA TAAACGGCCG TATAGCTTTA CTGACGATAG AATTGCTGCA 
AATGATGTGA GCAGGGAAAA TGCATTTTTG GGCAGTACAT TGGTTCGTGC AGTACCTGGG 
TTCATGACTC CAACGGAATG TGTCGAAGAG AAAATAAGGC CATTCGATTG TATTGGATTC 
CTCGAGACAA TCGATACGGA AAGAAAGAAA ATCTGCTATG TACCGGTTCC GCATAGTGGG 
AATATCAGTT TATACCAAGA CTGGGTAGCT TCAATCACCA AATCCGCATT CCATATTGTT 
CCATGGGACA ATGATAAAGT TCTCACTGTT GACAGCTCTG GAAGCATTCG TTCTTTCGAA 
TTAAGAATGT CGACATTGGA AAAATCATTT GATGACTGGA AGCGGATGAC TGGAGGGGCG 
GATGATGAGA AGTTGAGAAT GGAGTTTGAT AGAAAACCAG ACGACGTAGA CTTTAATAAA 
CTTGATGAGC CAAAACTCGG AAAGATCGAC CCCAACAATG CACCTCATCA CGGTGGAAAT 
CAATGGATGG GAGGAACTGG TGGATACAAT ACTGCAGGTC TCGGAGGAAT TGGAGGGCCG 
TTCAGACTTG ACGCAGGTCA TGATGTCCAT CAAATGCCAG ATTTTGCTAA GCAACAAGTC 
CCGAAGCACA TATTGAAAAA AGCCCGGGAG ATTGCAAAAG TAGAGTATGC AAAGAAATTG 
AGGGAAATCA ACATGTCGGA GTATGACGCC GATGCCTATG AAAAAGTGTG GAACAAAGTT 
CAAGCTCCAT CAAGAAAACT AGCATCTGTG ATTGACCAAT TGGAGGCAAA GAAAAAAGAA 
AGAGAATGGA CAAAGCATCA AACTAGCGGA GATTTGGATG ACGGAAAATT GATTGAAGGA 
GTCACCGGAG AGCAGAATAT TTACAGGCGA CGCATCGACA AAGTTCCAGA TCCGGGAGCT 
CCACAAACAA AGCCAAAACG CCTGAAAGTA TGCTTGGATG TTTCTGGTTC CATGTACAGA 
TTTAATGGAT ATGACCAGAG ACTCGTGAAG TCACTTGAAG CAGCCTTGAT GACAATGACT 
GCACTTGATG GAAAAACGGA CAAAGTGCAG TACGACATTA TTGGACATTC TGGAGACTCT 
CCATGCGTGT CATTTGTAAA GACCGATCAT CATCCCAAAA ACAACAAAGA ACGTCTGGAT 
ACATTGAAAC GCATGATTGC GCATACGCAG TACTGCGTCA GTGGTGACAA TACGGTAGAG 
AGTTTACAAT TTGCAATCAA AGAATTGGCC GCAAAGAAAG ATGATTTTGA TGAAACCGTT 
GTCATTTTGG TGTCAGATGC TAATTTGGAA AGGTATGGAA TTCAACCAAA GGAATTGAAA 
GATGCAATGG CTAAAGAGCC AAATATCAAC TCATTTGTCA TTTTCATTGG GTCGCTCAGT 
GACGAAGCCG ACCAGCTACA ACGAGAGTTG CCTGTTGGAA AAGCTTTTGT TTTAAAAGAC 
ACTTCTGAGC TTCCAAAAAT TATGGAGACC ATTTTCTCAT CAACTATTGC TCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa-8 ( NM_077759.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510160.2 Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

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CloneID	OCa355671
Clone ID Related Accession (Same CDS sequence)	NM_077759.4 , NM_077759.6
Accession Version	NM_077759.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Von (von) Willebrand factor A domain containing
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_077759.3. COMPLETENESS: incomplete on both ends.

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGGTTGACG AAATCAACAT ACACAAGATT 
ATCCCTCGTT TTCGTGCAAT TAACAAACCA CGAATTAGAC TAGCATATCT TGGTGGAGAC 
AATGTGCTGC TACACGAAGA AGAGGCGAAC TTCACAGCGA TCATCAGCTT GCCCGACAAA 
ATGGCGGCTG TTGTTACAAA GCAAACGAGT TTGGGTGAAA CGCTTGGAGC TATGTTTACC 
GGTTCCAATT ATTCATGGAG ATTATCAAAT CAACAAAGCA ATCACGCTTT GCTTTATGAG 
TGTGGAGGGC GCGAAATGCT GGTGTATACC GATGAAAAAC ACGGAGTCAC TGTCCAGAAC 
GGCACGGTAC CGTTTAACAT CAAACGAGTA TTTGCTTCAG CTCCTCAACA ATGGACTGTA 
GTTTCAATGG AAAATGAGAA CTATCTTCTG AAGGTGGAAC TCGGAAATTT TACTTTGGAG 
AAAATTGACA ACGATTTGGA AGGCGGCCTA GATACGGTTG CAACAAAGTT GGACAAGAAT 
GCAATGACTT TAGTTGCCGA GCCATACTAC TTTGTTCAAG GAAACCATTC TGGAAGTGTT 
TCCGGCGTAC TCCGAAAGAA ATCTGAAAAT ACGTTTCATA AACGGCCGTA TAGCTTTACT 
GACGATAGAA TTGCTGCAAA TGATGTGAGC AGGGAAAATG CATTTTTGGG CAGTACATTG 
GTTCGTGCAG TACCTGGGTT CATGACTCCA ACGGAATGTG TCGAAGAGAA AATAAGGCCA 
TTCGATTGTA TTGGATTCCT CGAGACAATC GATACGGAAA GAAAGAAAAT CTGCTATGTA 
CCGGTTCCGC ATAGTGGGAA TATCAGTTTA TACCAAGACT GGGTAGCTTC AATCACCAAA 
TCCGCATTCC ATATTGTTCC ATGGGACAAT GATAAAGTTC TCACTGTTGA CAGCTCTGGA 
AGCATTCGTT CTTTCGAATT AAGAATGTCG ACATTGGAAA AATCATTTGA TGACTGGAAG 
CGGATGACTG GAGGGGCGGA TGATGAGAAG TTGAGAATGG AGTTTGATAG AAAACCAGAC 
GACGTAGACT TTAATAAACT TGATGAGCCA AAACTCGGAA AGATCGACCC CAACAATGCA 
CCTCATCACG GTGGAAATCA ATGGATGGGA GGAACTGGTG GATACAATAC TGCAGGTCTC 
GGAGGAATTG GAGGGCCGTT CAGACTTGAC GCAGGTCATG ATGTCCATCA AATGCCAGAT 
TTTGCTAAGC AACAAGTCCC GAAGCACATA TTGAAAAAAG CCCGGGAGAT TGCAAAAGTA 
GAGTATGCAA AGAAATTGAG GGAAATCAAC ATGTCGGAGT ATGACGCCGA TGCCTATGAA 
AAAGTGTGGA ACAAAGTTCA AGCTCCATCA AGAAAACTAG CATCTGTGAT TGACCAATTG 
GAGGCAAAGA AAAAAGAAAG AGAATGGACA AAGCATCAAA CTAGCGGAGA TTTGGATGAC 
GGAAAATTGA TTGAAGGAGT CACCGGAGAG CAGAATATTT ACAGGCGACG CATCGACAAA 
GTTCCAGATC CGGGAGCTCC ACAAACAAAG CCAAAACGCC TGAAAGTATG CTTGGATGTT 
TCTGGTTCCA TGTACAGATT TAATGGATAT GACCAGAGAC TCGTGAAGTC ACTTGAAGCA 
GCCTTGATGA CAATGACTGC ACTTGATGGA AAAACGGACA AAGTGCAGTA CGACATTATT 
GGACATTCTG GAGACTCTCC ATGCGTGTCA TTTGTAAAGA CCGATCATCA TCCCAAAAAC 
AACAAAGAAC GTCTGGATAC ATTGAAACGC ATGATTGCGC ATACGCAGTA CTGCGTCAGT 
GGTGACAATA CGGTAGAGAG TTTACAATTT GCAATCAAAG AATTGGCCGC AAAGAAAGAT 
GATTTTGATG AAACCGTTGT CATTTTGGTG TCAGATGCTA ATTTGGAAAG GTATGGAATT 
CAACCAAAGG AATTGAAAGA TGCAATGGCT AAAGAGCCAA ATATCAACTC ATTTGTCATT 
TTCATTGGGT CGCTCAGTGA CGAAGCCGAC CAGCTACAAC GAGAGTTGCC TGTTGGAAAA 
GCTTTTGTTT TAAAAGACAC TTCTGAGCTT CCAAAAATTA TGGAGACCAT TTTCTCATCA 
ACTATTGCTC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510160.2
CDS	1..5415
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGVDEATMNERGVFQIPDSFRVLFVGESQSKEHKWINENVLSILPFYTVPNLSLSEQMSIISEITHGSNTESVDKLIKFVQTIKNSNDAGLKNTATSLSLRRLIHIAKRDLMQPGHLRELVEKAALSKFLPQITKETFEHELNTANVFNDTTIRTKENEKFLDMIRANTIKAEDEALIPNVLFHHNKQHDEILEDMARDMKLGSHLLLIGNQGVGKNKLTDRFLHLINRPRQYMQLHRDTTVQTLTMQTVVENGIIRHEDSALVKAARSGQILVIDEADKAPLHVIAILKSLLDTGTLVLGDGRTLRPASSFTEADKTNDRIVPIHPNFRIIMLANRPGFPFLGNNLFAVLGDLFAIHMIDHPGRDSELAMIKKFGPTVPVKTLNQLLTIFNELREKTDQGLLQYPYSTRELVNIVRHCNEFPTDPLPEVCRNVFDFDSYSEDTIATIHEVFQKHGVPLGVIRTGESLSIMERIPLGEMKTIGKWSRQKVEFPKSYGFKTPVAVEIERPEKMEFGLRKLDKQSNRNNVFSELHCVWKLPTDESNIISDGIRINDDLLVASVNPPRLYHCKDFMDTESVDEINIHKIIPRFRAINKPRIRLAYLGGDNVLLHEEEANFTAIISLPDKMAAVVTKQTSLGETLGAMFTGSNYSWRLSNQQSNHALLYECGGREMLVYTDEKHGVTVQNGTVPFNIKRVFASAPQQWTVVSMENENYLLKVELGNFTLEKIDNDLEGGLDTVATKLDKNAMTLVAEPYYFVQGNHSGSVSGVLRKKSENTFHKRPYSFTDDRIAANDVSRENAFLGSTLVRAVPGFMTPTECVEEKIRPFDCIGFLETIDTERKKICYVPVPHSGNISLYQDWVASITKSAFHIVPWDNDKVLTVDSSGSIRSFELRMSTLEKSFDDWKRMTGGADDEKLRMEFDRKPDDVDFNKLDEPKLGKIDPNNAPHHGGNQWMGGTGGYNTAGLGGIGGPFRLDAGHDVHQMPDFAKQQVPKHILKKAREIAKVEYAKKLREINMSEYDADAYEKVWNKVQAPSRKLASVIDQLEAKKKEREWTKHQTSGDLDDGKLIEGVTGEQNIYRRRIDKVPDPGAPQTKPKRLKVCLDVSGSMYRFNGYDQRLVKSLEAALMTMTALDGKTDKVQYDIIGHSGDSPCVSFVKTDHHPKNNKERLDTLKRMIAHTQYCVSGDNTVESLQFAIKELAAKKDDFDETVVILVSDANLERYGIQPKELKDAMAKEPNINSFVIFIGSLSDEADQLQRELPVGKAFVLKDTSELPKIMETIFSSTIAQ

Target ORF information:

RefSeq Version	NM_077759.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Von (von) Willebrand factor A domain containing (vwa-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077759.4

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGGTTGACG AAATCAACAT ACACAAGATT 
ATCCCTCGTT TTCGTGCAAT TAACAAACCA CGAATTAGAC TAGCATATCT TGGTGGAGAC 
AATGTGCTGC TACACGAAGA AGAGGCGAAC TTCACAGCGA TCATCAGCTT GCCCGACAAA 
ATGGCGGCTG TTGTTACAAA GCAAACGAGT TTGGGTGAAA CGCTTGGAGC TATGTTTACC 
GGTTCCAATT ATTCATGGAG ATTATCAAAT CAACAAAGCA ATCACGCTTT GCTTTATGAG 
TGTGGAGGGC GCGAAATGCT GGTGTATACC GATGAAAAAC ACGGAGTCAC TGTCCAGAAC 
GGCACGGTAC CGTTTAACAT CAAACGAGTA TTTGCTTCAG CTCCTCAACA ATGGACTGTA 
GTTTCAATGG AAAATGAGAA CTATCTTCTG AAGGTGGAAC TCGGAAATTT TACTTTGGAG 
AAAATTGACA ACGATTTGGA AGGCGGCCTA GATACGGTTG CAACAAAGTT GGACAAGAAT 
GCAATGACTT TAGTTGCCGA GCCATACTAC TTTGTTCAAG GAAACCATTC TGGAAGTGTT 
TCCGGCGTAC TCCGAAAGAA ATCTGAAAAT ACGTTTCATA AACGGCCGTA TAGCTTTACT 
GACGATAGAA TTGCTGCAAA TGATGTGAGC AGGGAAAATG CATTTTTGGG CAGTACATTG 
GTTCGTGCAG TACCTGGGTT CATGACTCCA ACGGAATGTG TCGAAGAGAA AATAAGGCCA 
TTCGATTGTA TTGGATTCCT CGAGACAATC GATACGGAAA GAAAGAAAAT CTGCTATGTA 
CCGGTTCCGC ATAGTGGGAA TATCAGTTTA TACCAAGACT GGGTAGCTTC AATCACCAAA 
TCCGCATTCC ATATTGTTCC ATGGGACAAT GATAAAGTTC TCACTGTTGA CAGCTCTGGA 
AGCATTCGTT CTTTCGAATT AAGAATGTCG ACATTGGAAA AATCATTTGA TGACTGGAAG 
CGGATGACTG GAGGGGCGGA TGATGAGAAG TTGAGAATGG AGTTTGATAG AAAACCAGAC 
GACGTAGACT TTAATAAACT TGATGAGCCA AAACTCGGAA AGATCGACCC CAACAATGCA 
CCTCATCACG GTGGAAATCA ATGGATGGGA GGAACTGGTG GATACAATAC TGCAGGTCTC 
GGAGGAATTG GAGGGCCGTT CAGACTTGAC GCAGGTCATG ATGTCCATCA AATGCCAGAT 
TTTGCTAAGC AACAAGTCCC GAAGCACATA TTGAAAAAAG CCCGGGAGAT TGCAAAAGTA 
GAGTATGCAA AGAAATTGAG GGAAATCAAC ATGTCGGAGT ATGACGCCGA TGCCTATGAA 
AAAGTGTGGA ACAAAGTTCA AGCTCCATCA AGAAAACTAG CATCTGTGAT TGACCAATTG 
GAGGCAAAGA AAAAAGAAAG AGAATGGACA AAGCATCAAA CTAGCGGAGA TTTGGATGAC 
GGAAAATTGA TTGAAGGAGT CACCGGAGAG CAGAATATTT ACAGGCGACG CATCGACAAA 
GTTCCAGATC CGGGAGCTCC ACAAACAAAG CCAAAACGCC TGAAAGTATG CTTGGATGTT 
TCTGGTTCCA TGTACAGATT TAATGGATAT GACCAGAGAC TCGTGAAGTC ACTTGAAGCA 
GCCTTGATGA CAATGACTGC ACTTGATGGA AAAACGGACA AAGTGCAGTA CGACATTATT 
GGACATTCTG GAGACTCTCC ATGCGTGTCA TTTGTAAAGA CCGATCATCA TCCCAAAAAC 
AACAAAGAAC GTCTGGATAC ATTGAAACGC ATGATTGCGC ATACGCAGTA CTGCGTCAGT 
GGTGACAATA CGGTAGAGAG TTTACAATTT GCAATCAAAG AATTGGCCGC AAAGAAAGAT 
GATTTTGATG AAACCGTTGT CATTTTGGTG TCAGATGCTA ATTTGGAAAG GTATGGAATT 
CAACCAAAGG AATTGAAAGA TGCAATGGCT AAAGAGCCAA ATATCAACTC ATTTGTCATT 
TTCATTGGGT CGCTCAGTGA CGAAGCCGAC CAGCTACAAC GAGAGTTGCC TGTTGGAAAA 
GCTTTTGTTT TAAAAGACAC TTCTGAGCTT CCAAAAATTA TGGAGACCAT TTTCTCATCA 
ACTATTGCTC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa-8 ( NM_077759.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510160.2 Caenorhabditis elegans VWFA domain-containing protein (vwa-8), mRNA.

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CloneID	OCa355671
Clone ID Related Accession (Same CDS sequence)	NM_077759.4 , NM_077759.6
Accession Version	NM_077759.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	VWFA domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_077759.5.

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGGTTGACG AAATCAACAT ACACAAGATT 
ATCCCTCGTT TTCGTGCAAT TAACAAACCA CGAATTAGAC TAGCATATCT TGGTGGAGAC 
AATGTGCTGC TACACGAAGA AGAGGCGAAC TTCACAGCGA TCATCAGCTT GCCCGACAAA 
ATGGCGGCTG TTGTTACAAA GCAAACGAGT TTGGGTGAAA CGCTTGGAGC TATGTTTACC 
GGTTCCAATT ATTCATGGAG ATTATCAAAT CAACAAAGCA ATCACGCTTT GCTTTATGAG 
TGTGGAGGGC GCGAAATGCT GGTGTATACC GATGAAAAAC ACGGAGTCAC TGTCCAGAAC 
GGCACGGTAC CGTTTAACAT CAAACGAGTA TTTGCTTCAG CTCCTCAACA ATGGACTGTA 
GTTTCAATGG AAAATGAGAA CTATCTTCTG AAGGTGGAAC TCGGAAATTT TACTTTGGAG 
AAAATTGACA ACGATTTGGA AGGCGGCCTA GATACGGTTG CAACAAAGTT GGACAAGAAT 
GCAATGACTT TAGTTGCCGA GCCATACTAC TTTGTTCAAG GAAACCATTC TGGAAGTGTT 
TCCGGCGTAC TCCGAAAGAA ATCTGAAAAT ACGTTTCATA AACGGCCGTA TAGCTTTACT 
GACGATAGAA TTGCTGCAAA TGATGTGAGC AGGGAAAATG CATTTTTGGG CAGTACATTG 
GTTCGTGCAG TACCTGGGTT CATGACTCCA ACGGAATGTG TCGAAGAGAA AATAAGGCCA 
TTCGATTGTA TTGGATTCCT CGAGACAATC GATACGGAAA GAAAGAAAAT CTGCTATGTA 
CCGGTTCCGC ATAGTGGGAA TATCAGTTTA TACCAAGACT GGGTAGCTTC AATCACCAAA 
TCCGCATTCC ATATTGTTCC ATGGGACAAT GATAAAGTTC TCACTGTTGA CAGCTCTGGA 
AGCATTCGTT CTTTCGAATT AAGAATGTCG ACATTGGAAA AATCATTTGA TGACTGGAAG 
CGGATGACTG GAGGGGCGGA TGATGAGAAG TTGAGAATGG AGTTTGATAG AAAACCAGAC 
GACGTAGACT TTAATAAACT TGATGAGCCA AAACTCGGAA AGATCGACCC CAACAATGCA 
CCTCATCACG GTGGAAATCA ATGGATGGGA GGAACTGGTG GATACAATAC TGCAGGTCTC 
GGAGGAATTG GAGGGCCGTT CAGACTTGAC GCAGGTCATG ATGTCCATCA AATGCCAGAT 
TTTGCTAAGC AACAAGTCCC GAAGCACATA TTGAAAAAAG CCCGGGAGAT TGCAAAAGTA 
GAGTATGCAA AGAAATTGAG GGAAATCAAC ATGTCGGAGT ATGACGCCGA TGCCTATGAA 
AAAGTGTGGA ACAAAGTTCA AGCTCCATCA AGAAAACTAG CATCTGTGAT TGACCAATTG 
GAGGCAAAGA AAAAAGAAAG AGAATGGACA AAGCATCAAA CTAGCGGAGA TTTGGATGAC 
GGAAAATTGA TTGAAGGAGT CACCGGAGAG CAGAATATTT ACAGGCGACG CATCGACAAA 
GTTCCAGATC CGGGAGCTCC ACAAACAAAG CCAAAACGCC TGAAAGTATG CTTGGATGTT 
TCTGGTTCCA TGTACAGATT TAATGGATAT GACCAGAGAC TCGTGAAGTC ACTTGAAGCA 
GCCTTGATGA CAATGACTGC ACTTGATGGA AAAACGGACA AAGTGCAGTA CGACATTATT 
GGACATTCTG GAGACTCTCC ATGCGTGTCA TTTGTAAAGA CCGATCATCA TCCCAAAAAC 
AACAAAGAAC GTCTGGATAC ATTGAAACGC ATGATTGCGC ATACGCAGTA CTGCGTCAGT 
GGTGACAATA CGGTAGAGAG TTTACAATTT GCAATCAAAG AATTGGCCGC AAAGAAAGAT 
GATTTTGATG AAACCGTTGT CATTTTGGTG TCAGATGCTA ATTTGGAAAG GTATGGAATT 
CAACCAAAGG AATTGAAAGA TGCAATGGCT AAAGAGCCAA ATATCAACTC ATTTGTCATT 
TTCATTGGGT CGCTCAGTGA CGAAGCCGAC CAGCTACAAC GAGAGTTGCC TGTTGGAAAA 
GCTTTTGTTT TAAAAGACAC TTCTGAGCTT CCAAAAATTA TGGAGACCAT TTTCTCATCA 
ACTATTGCTC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510160.2
CDS	24..5438
Translation	MITRVSLLRLQHARKVLTTTASTSSKPSTLTIGSVSVQLQDAKNPDFVPAFYGQKNNPPQDLLPHLRWLIQKDKLKQDVFLLGVPGKIRLELVLRYLEATNREFEYLPITRDTTEADIKQRREIRDGTAYYTDLCAVRAALKGRVLVIDGVERAERNVLPILNNLLENREMQLDDGRFLMKHDKYDELKVKYDEATLKKMGMERVSENFHVIALGLPVPRFPGNSLDPPFRSRFQCRNIQELSFHTFLEQAKLIAPNVSETNLNDLISVVYAYNSEASTSRPRIGTAVVENTLKIWNENPSYSAGEIVNFSYPAKEVLSDSEKDLINTFKTKFELSTHERTRELKTDGALHTVQVPADKRKIQMKLPTPSHPTSFASTWIPTAKQENLIADLALAFSKGDFILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQRRHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEKEFLTIMERDGVDEATMNERGVFQIPDSFRVLFVGESQSKEHKWINENVLSILPFYTVPNLSLSEQMSIISEITHGSNTESVDKLIKFVQTIKNSNDAGLKNTATSLSLRRLIHIAKRDLMQPGHLRELVEKAALSKFLPQITKETFEHELNTANVFNDTTIRTKENEKFLDMIRANTIKAEDEALIPNVLFHHNKQHDEILEDMARDMKLGSHLLLIGNQGVGKNKLTDRFLHLINRPRQYMQLHRDTTVQTLTMQTVVENGIIRHEDSALVKAARSGQILVIDEADKAPLHVIAILKSLLDTGTLVLGDGRTLRPASSFTEADKTNDRIVPIHPNFRIIMLANRPGFPFLGNNLFAVLGDLFAIHMIDHPGRDSELAMIKKFGPTVPVKTLNQLLTIFNELREKTDQGLLQYPYSTRELVNIVRHCNEFPTDPLPEVCRNVFDFDSYSEDTIATIHEVFQKHGVPLGVIRTGESLSIMERIPLGEMKTIGKWSRQKVEFPKSYGFKTPVAVEIERPEKMEFGLRKLDKQSNRNNVFSELHCVWKLPTDESNIISDGIRINDDLLVASVNPPRLYHCKDFMDTESVDEINIHKIIPRFRAINKPRIRLAYLGGDNVLLHEEEANFTAIISLPDKMAAVVTKQTSLGETLGAMFTGSNYSWRLSNQQSNHALLYECGGREMLVYTDEKHGVTVQNGTVPFNIKRVFASAPQQWTVVSMENENYLLKVELGNFTLEKIDNDLEGGLDTVATKLDKNAMTLVAEPYYFVQGNHSGSVSGVLRKKSENTFHKRPYSFTDDRIAANDVSRENAFLGSTLVRAVPGFMTPTECVEEKIRPFDCIGFLETIDTERKKICYVPVPHSGNISLYQDWVASITKSAFHIVPWDNDKVLTVDSSGSIRSFELRMSTLEKSFDDWKRMTGGADDEKLRMEFDRKPDDVDFNKLDEPKLGKIDPNNAPHHGGNQWMGGTGGYNTAGLGGIGGPFRLDAGHDVHQMPDFAKQQVPKHILKKAREIAKVEYAKKLREINMSEYDADAYEKVWNKVQAPSRKLASVIDQLEAKKKEREWTKHQTSGDLDDGKLIEGVTGEQNIYRRRIDKVPDPGAPQTKPKRLKVCLDVSGSMYRFNGYDQRLVKSLEAALMTMTALDGKTDKVQYDIIGHSGDSPCVSFVKTDHHPKNNKERLDTLKRMIAHTQYCVSGDNTVESLQFAIKELAAKKDDFDETVVILVSDANLERYGIQPKELKDAMAKEPNINSFVIFIGSLSDEADQLQRELPVGKAFVLKDTSELPKIMETIFSSTIAQ

Target ORF information:

RefSeq Version	NM_077759.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans VWFA domain-containing protein (vwa-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077759.6

ORF Insert Sequence:

ATGATCACAC GGGTTTCACT TCTCCGCTTG CAGCACGCAA GAAAGGTTTT AACAACTACA 
GCATCTACTA GCTCCAAACC ATCGACGCTG ACTATCGGAA GTGTTAGTGT ACAACTTCAA 
GATGCAAAGA ACCCAGACTT TGTCCCAGCC TTCTATGGGC AGAAAAACAA TCCCCCACAG 
GATTTGCTCC CACATTTGAG ATGGCTCATT CAAAAAGACA AATTAAAGCA AGACGTGTTT 
CTGCTGGGAG TGCCGGGAAA AATCAGATTG GAACTTGTCT TGAGATATTT GGAAGCTACA 
AATCGAGAGT TTGAGTACCT ACCAATCACT AGAGACACCA CCGAAGCAGA TATCAAACAG 
CGACGTGAAA TTAGAGATGG AACTGCATAT TACACAGACT TGTGTGCGGT TCGTGCAGCA 
TTGAAAGGAA GAGTGCTAGT AATTGATGGT GTGGAAAGAG CCGAACGAAA CGTGCTTCCA 
ATCCTGAACA ATCTTTTGGA AAATCGAGAA ATGCAATTGG ACGATGGAAG ATTTTTGATG 
AAGCATGACA AATACGATGA GCTGAAGGTG AAATACGATG AAGCGACACT GAAGAAGATG 
GGAATGGAGC GAGTTTCTGA AAACTTCCAT GTCATAGCCC TTGGTCTTCC CGTACCAAGA 
TTCCCGGGAA ATTCTTTGGA TCCGCCGTTC CGAAGCAGAT TCCAATGCAG AAATATTCAG 
GAGCTATCGT TCCACACATT TTTGGAACAA GCGAAGCTCA TTGCTCCAAA CGTTAGTGAG 
ACAAATCTTA ACGATTTGAT ATCCGTTGTG TATGCCTATA ATTCGGAAGC GTCAACTTCA 
AGACCAAGAA TCGGCACAGC TGTCGTAGAG AATACATTGA AGATTTGGAA CGAGAATCCC 
AGCTATTCCG CAGGAGAAAT TGTCAACTTC TCATATCCTG CCAAAGAAGT TCTATCTGAC 
AGTGAAAAAG ATCTTATCAA CACATTCAAG ACAAAGTTTG AATTGAGTAC TCATGAACGC 
ACCAGAGAAC TGAAAACGGA CGGTGCTTTA CATACGGTTC AAGTTCCTGC TGACAAGAGA 
AAGATCCAGA TGAAGCTGCC AACACCATCA CATCCTACTT CATTTGCGTC TACTTGGATT 
CCAACCGCAA AGCAAGAAAA TCTGATCGCG GATCTTGCTC TAGCATTTTC GAAAGGAGAC 
TTTATCCTAA TTGGACCAAA AGGCTCTGGG AAATCGAGAA TCCTTGGCGA ACTTTCACGA 
CGTCTCAATT TCAATTATGT CACAATGGTT CTGCATCAAG ATATGAACAC CAGAGAGCTT 
ATCCAACGCA GACATATGAA AGAAAATGGC GATACAGTTT GGGAAGACTC GATTCTTGTG 
ACTGCAGCAA GAAATGGAGA TGTCTGTGTT TTGGATGGTG TCGAAATGGT GCATTCTAGT 
GTTATTATGT CACTCTCGCA GTTGATTTAT CATCGAAGAT TTGATCTACC AAATGGAAAT 
CGTCTTGTTG GAGAGAAAGA GTTTTTGACT ATTATGGAAA GAGATGGAGT TGATGAAGCA 
ACAATGAACG AAAGAGGTGT ATTCCAAATT CCTGACTCCT TCCGCGTTTT GTTTGTTGGT 
GAGTCCCAGT CGAAAGAGCA TAAATGGATC AACGAAAATG TGCTCTCCAT CCTGCCATTC 
TACACTGTCC CAAATCTATC GCTCTCCGAG CAAATGAGTA TCATTTCGGA AATCACCCAT 
GGATCCAACA CTGAATCTGT GGACAAACTT ATCAAGTTTG TGCAAACTAT CAAAAACTCC 
AATGATGCTG GGTTAAAAAA TACTGCAACA AGTTTGTCGC TTCGTCGATT GATTCACATT 
GCAAAACGAG ATCTGATGCA GCCGGGACAC TTGAGAGAGC TTGTAGAAAA AGCTGCTCTC 
TCAAAGTTCC TCCCACAAAT CACCAAGGAA ACATTCGAAC ATGAGCTCAA CACTGCAAAC 
GTCTTTAATG ATACTACTAT TCGCACAAAG GAGAATGAGA AGTTCCTTGA CATGATTCGA 
GCAAACACTA TAAAAGCGGA AGATGAAGCT CTTATTCCAA ATGTGCTATT CCACCATAAT 
AAGCAGCACG ACGAAATTCT AGAGGACATG GCAAGAGACA TGAAACTGGG AAGTCATTTG 
CTTTTGATCG GTAACCAAGG AGTCGGAAAA AATAAACTCA CTGATAGGTT CCTTCACCTA 
ATAAATCGAC CGAGACAGTA CATGCAGCTT CACAGAGATA CCACAGTACA AACGTTAACA 
ATGCAGACCG TAGTAGAAAA TGGAATCATT CGTCATGAAG ATAGTGCGTT GGTGAAGGCA 
GCACGAAGTG GTCAGATTCT TGTGATTGAT GAAGCTGACA AAGCGCCTCT ACACGTGATC 
GCTATTTTGA AAAGTCTTCT CGACACAGGA ACATTGGTGC TTGGAGATGG GAGAACACTT 
CGTCCAGCAA GTTCTTTCAC AGAAGCTGAT AAAACAAATG ATCGCATTGT TCCAATTCAT 
CCCAACTTTA GAATTATCAT GCTTGCCAAC AGACCTGGAT TCCCATTCCT TGGCAACAAT 
TTGTTTGCTG TACTTGGTGA TCTGTTTGCA ATTCACATGA TTGATCACCC AGGAAGAGAT 
AGTGAACTCG CAATGATTAA GAAATTTGGA CCAACGGTTC CTGTAAAAAC TCTTAACCAA 
CTTTTAACAA TTTTCAACGA GCTGCGCGAA AAAACTGACC AAGGACTTTT ACAGTATCCT 
TACTCGACTC GAGAACTTGT TAATATTGTT CGCCATTGCA ATGAATTCCC AACGGATCCC 
CTACCAGAAG TTTGTCGAAA TGTGTTTGAC TTTGATTCAT ACAGTGAAGA CACCATTGCC 
ACAATTCATG AAGTATTCCA GAAGCACGGA GTACCTTTAG GTGTAATAAG AACCGGTGAA 
AGTCTGTCTA TAATGGAGAG AATCCCATTG GGAGAAATGA AAACTATCGG AAAATGGTCA 
AGACAAAAAG TCGAATTTCC AAAGTCGTAT GGGTTCAAGA CGCCGGTGGC TGTGGAAATT 
GAGCGGCCAG AAAAAATGGA ATTCGGTTTA CGCAAGTTGG ACAAACAGAG CAACAGAAAC 
AATGTTTTCT CGGAGTTGCA TTGTGTCTGG AAGTTGCCTA CTGACGAGTC AAACATAATT 
TCGGATGGTA TTCGTATCAA TGATGACTTG CTTGTTGCAA GTGTGAATCC TCCACGCCTA 
TATCATTGCA AAGATTTTAT GGATACAGAA TCGGTTGACG AAATCAACAT ACACAAGATT 
ATCCCTCGTT TTCGTGCAAT TAACAAACCA CGAATTAGAC TAGCATATCT TGGTGGAGAC 
AATGTGCTGC TACACGAAGA AGAGGCGAAC TTCACAGCGA TCATCAGCTT GCCCGACAAA 
ATGGCGGCTG TTGTTACAAA GCAAACGAGT TTGGGTGAAA CGCTTGGAGC TATGTTTACC 
GGTTCCAATT ATTCATGGAG ATTATCAAAT CAACAAAGCA ATCACGCTTT GCTTTATGAG 
TGTGGAGGGC GCGAAATGCT GGTGTATACC GATGAAAAAC ACGGAGTCAC TGTCCAGAAC 
GGCACGGTAC CGTTTAACAT CAAACGAGTA TTTGCTTCAG CTCCTCAACA ATGGACTGTA 
GTTTCAATGG AAAATGAGAA CTATCTTCTG AAGGTGGAAC TCGGAAATTT TACTTTGGAG 
AAAATTGACA ACGATTTGGA AGGCGGCCTA GATACGGTTG CAACAAAGTT GGACAAGAAT 
GCAATGACTT TAGTTGCCGA GCCATACTAC TTTGTTCAAG GAAACCATTC TGGAAGTGTT 
TCCGGCGTAC TCCGAAAGAA ATCTGAAAAT ACGTTTCATA AACGGCCGTA TAGCTTTACT 
GACGATAGAA TTGCTGCAAA TGATGTGAGC AGGGAAAATG CATTTTTGGG CAGTACATTG 
GTTCGTGCAG TACCTGGGTT CATGACTCCA ACGGAATGTG TCGAAGAGAA AATAAGGCCA 
TTCGATTGTA TTGGATTCCT CGAGACAATC GATACGGAAA GAAAGAAAAT CTGCTATGTA 
CCGGTTCCGC ATAGTGGGAA TATCAGTTTA TACCAAGACT GGGTAGCTTC AATCACCAAA 
TCCGCATTCC ATATTGTTCC ATGGGACAAT GATAAAGTTC TCACTGTTGA CAGCTCTGGA 
AGCATTCGTT CTTTCGAATT AAGAATGTCG ACATTGGAAA AATCATTTGA TGACTGGAAG 
CGGATGACTG GAGGGGCGGA TGATGAGAAG TTGAGAATGG AGTTTGATAG AAAACCAGAC 
GACGTAGACT TTAATAAACT TGATGAGCCA AAACTCGGAA AGATCGACCC CAACAATGCA 
CCTCATCACG GTGGAAATCA ATGGATGGGA GGAACTGGTG GATACAATAC TGCAGGTCTC 
GGAGGAATTG GAGGGCCGTT CAGACTTGAC GCAGGTCATG ATGTCCATCA AATGCCAGAT 
TTTGCTAAGC AACAAGTCCC GAAGCACATA TTGAAAAAAG CCCGGGAGAT TGCAAAAGTA 
GAGTATGCAA AGAAATTGAG GGAAATCAAC ATGTCGGAGT ATGACGCCGA TGCCTATGAA 
AAAGTGTGGA ACAAAGTTCA AGCTCCATCA AGAAAACTAG CATCTGTGAT TGACCAATTG 
GAGGCAAAGA AAAAAGAAAG AGAATGGACA AAGCATCAAA CTAGCGGAGA TTTGGATGAC 
GGAAAATTGA TTGAAGGAGT CACCGGAGAG CAGAATATTT ACAGGCGACG CATCGACAAA 
GTTCCAGATC CGGGAGCTCC ACAAACAAAG CCAAAACGCC TGAAAGTATG CTTGGATGTT 
TCTGGTTCCA TGTACAGATT TAATGGATAT GACCAGAGAC TCGTGAAGTC ACTTGAAGCA 
GCCTTGATGA CAATGACTGC ACTTGATGGA AAAACGGACA AAGTGCAGTA CGACATTATT 
GGACATTCTG GAGACTCTCC ATGCGTGTCA TTTGTAAAGA CCGATCATCA TCCCAAAAAC 
AACAAAGAAC GTCTGGATAC ATTGAAACGC ATGATTGCGC ATACGCAGTA CTGCGTCAGT 
GGTGACAATA CGGTAGAGAG TTTACAATTT GCAATCAAAG AATTGGCCGC AAAGAAAGAT 
GATTTTGATG AAACCGTTGT CATTTTGGTG TCAGATGCTA ATTTGGAAAG GTATGGAATT 
CAACCAAAGG AATTGAAAGA TGCAATGGCT AAAGAGCCAA ATATCAACTC ATTTGTCATT 
TTCATTGGGT CGCTCAGTGA CGAAGCCGAC CAGCTACAAC GAGAGTTGCC TGTTGGAAAA 
GCTTTTGTTT TAAAAGACAC TTCTGAGCTT CCAAAAATTA TGGAGACCAT TTTCTCATCA 
ACTATTGCTC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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vwa-8 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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