pyc-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	pyc-1
Entrez Gene ID	179616
Full Name	PYruvate Carboxylase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001269448.2	NP_001256377.1	Pyruvate carboxylase 1
NM_001269447.1	NP_001256376.1	Pyruvate carboxylase 1
NM_001269448.1	NP_001256377.1	Pyruvate carboxylase 1
NM_001269447.2	NP_001256376.1	Pyruvate carboxylase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pyc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pyc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa347798	NM_001269448.2 Latest version!	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa345711	NM_001269447.2 Latest version!	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa347798	NM_001269448.1	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa345711	NM_001269447.1	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pyc-1 ( NM_001269448.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256377.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

Price & Availability↑

CloneID	OCa347798
Clone ID Related Accession (Same CDS sequence)	NM_001269448.2 , NM_001269448.1
Accession Version	NM_001269448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Pyruvate carboxylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269448.1. COMPLETENESS: incomplete on the 5' end.

ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256377.1
CDS	1..1851
Translation	MITDTTFRDAHQSLLATRVRTYDMAAISPFVAQSFNGLFSLENWGGATFDVSMRFLHECPWERLQTLRKLIPNIPFQCLLRGANAMGYSNYPDNVIYKFCELAVKNGMDVFRVFDSLNYLPNLLVGMEAVGKAGGVVEAAIAYTGDVTDKSRDKYDLKYYLNLADQLVKAQAHILSIKDMAGVLKPEAAKLLIGALRDKFPDIPIHVHTHDTSGAGVAAMLECAKAGADVVDAAVDSMSGMTSQPSMGAIVASLQGTKHDTGLSLDDISKYSAYWESTRQLYAPFECATTMKSGNADVYKHEIPGGQYTNLQFQAFSLGLGPQFDEVKRMYREANLVLGDIIKVTPSSKIVGDLAQFMVQNNLTRETLVDRADDLSFPKSVVDFMQGNVGQPPYGFPEPLRTKVLRGKPKVDGRPGENAKPVDLDAVKVELEEKHGRTLSEEDVMSYSMFPTVFDEFETFRQQYGPVDKLPTRLFLTGLEIAEEVDVEIESGKTLAIQLLAEGKLNKRGEREVFFDLNGQMRSIFVVDKEASKEIVTRPRALPGVRGHIGAPMPGDVLELKIKEGDKVTKKQPLFVLSAMKMEMVIDSPIAGTVKAIHAPQGTKCSAGDLVVEVEP

Target ORF information:

RefSeq Version	NM_001269448.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269448.2

ORF Insert Sequence:

ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pyc-1 ( NM_001269448.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256377.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

Price & Availability↑

CloneID	OCa347798
Clone ID Related Accession (Same CDS sequence)	NM_001269448.2 , NM_001269448.1
Accession Version	NM_001269448.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Pyruvate carboxylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256377.1
CDS	1..1851
Translation	MITDTTFRDAHQSLLATRVRTYDMAAISPFVAQSFNGLFSLENWGGATFDVSMRFLHECPWERLQTLRKLIPNIPFQCLLRGANAMGYSNYPDNVIYKFCELAVKNGMDVFRVFDSLNYLPNLLVGMEAVGKAGGVVEAAIAYTGDVTDKSRDKYDLKYYLNLADQLVKAQAHILSIKDMAGVLKPEAAKLLIGALRDKFPDIPIHVHTHDTSGAGVAAMLECAKAGADVVDAAVDSMSGMTSQPSMGAIVASLQGTKHDTGLSLDDISKYSAYWESTRQLYAPFECATTMKSGNADVYKHEIPGGQYTNLQFQAFSLGLGPQFDEVKRMYREANLVLGDIIKVTPSSKIVGDLAQFMVQNNLTRETLVDRADDLSFPKSVVDFMQGNVGQPPYGFPEPLRTKVLRGKPKVDGRPGENAKPVDLDAVKVELEEKHGRTLSEEDVMSYSMFPTVFDEFETFRQQYGPVDKLPTRLFLTGLEIAEEVDVEIESGKTLAIQLLAEGKLNKRGEREVFFDLNGQMRSIFVVDKEASKEIVTRPRALPGVRGHIGAPMPGDVLELKIKEGDKVTKKQPLFVLSAMKMEMVIDSPIAGTVKAIHAPQGTKCSAGDLVVEVEP

Target ORF information:

RefSeq Version	NM_001269448.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269448.1

ORF Insert Sequence:

ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pyc-1 ( NM_001269447.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256376.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

Price & Availability↑

CloneID	OCa345711
Clone ID Related Accession (Same CDS sequence)	NM_001269447.1 , NM_001269447.2
Accession Version	NM_001269447.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Pyruvate carboxylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256376.1
CDS	1..3528
Translation	MRFSRIPPIFANVVRQTHYRNYANGVIKPREFNKVMVANRGEIAIRVFRALTELNKTSVAIYAEQDKNSMHRLKADEAYLVGKGLPPVAAYLTIDQIIETALKHNIDAIHPGYGFLSERSDFAAACQNAGIVFIGPSPDVMARMGDKVAARQAAIEAGVQVVPGTPGPITTADEAVEFAKQYGTPIILKAAYGGGGRGIRRVDKLEEVEEAFRRSYSEAQAAFGDGSLFVEKFVERPRHIEVQLLGDHHGNIVHLYERDCSVQRRHQKVVEIAPAPALPEGVREKILADALRLARHVGYQNAGTVEFLVDQKGNYYFIEVNARLQVEHTVTEEITGVDLVQAQIRIAEGKSLDDLKLSQETIQTTGSAIQCRVTTEDPAKGFQPDSGRIEVFRSGEGMGIRLDSASAFAGSVISPHYDSLMVKVIASARNHPNAAAKMIRALKKFRIRGVKTNIPFLLNVLRQPSFLDASVDTYFIDEHPELFQFKPSQNRAQKLLNYLGEVKVNGPTTPLATDLKPAVVSPPIPYIPAGAKPPTGLRDVLVQRGPTEFAKEVRSRPGCMITDTTFRDAHQSLLATRVRTYDMAAISPFVAQSFNGLFSLENWGGATFDVSMRFLHECPWERLQTLRKLIPNIPFQCLLRGANAMGYSNYPDNVIYKFCELAVKNGMDVFRVFDSLNYLPNLLVGMEAVGKAGGVVEAAIAYTGDVTDKSRDKYDLKYYLNLADQLVKAQAHILSIKDMAGVLKPEAAKLLIGALRDKFPDIPIHVHTHDTSGAGVAAMLECAKAGADVVDAAVDSMSGMTSQPSMGAIVASLQGTKHDTGLSLDDISKYSAYWESTRQLYAPFECATTMKSGNADVYKHEIPGGQYTNLQFQAFSLGLGPQFDEVKRMYREANLVLGDIIKVTPSSKIVGDLAQFMVQNNLTRETLVDRADDLSFPKSVVDFMQGNVGQPPYGFPEPLRTKVLRGKPKVDGRPGENAKPVDLDAVKVELEEKHGRTLSEEDVMSYSMFPTVFDEFETFRQQYGPVDKLPTRLFLTGLEIAEEVDVEIESGKTLAIQLLAEGKLNKRGEREVFFDLNGQMRSIFVVDKEASKEIVTRPRALPGVRGHIGAPMPGDVLELKIKEGDKVTKKQPLFVLSAMKMEMVIDSPIAGTVKAIHAPQGTKCSAGDLVVEVEP

Target ORF information:

RefSeq Version	NM_001269447.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269447.1

ORF Insert Sequence:

ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pyc-1 ( NM_001269447.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256376.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), mRNA.

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CloneID	OCa345711
Clone ID Related Accession (Same CDS sequence)	NM_001269447.1 , NM_001269447.2
Accession Version	NM_001269447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Pyruvate carboxylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269447.1.

ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256376.1
CDS	14..3541
Translation	MRFSRIPPIFANVVRQTHYRNYANGVIKPREFNKVMVANRGEIAIRVFRALTELNKTSVAIYAEQDKNSMHRLKADEAYLVGKGLPPVAAYLTIDQIIETALKHNIDAIHPGYGFLSERSDFAAACQNAGIVFIGPSPDVMARMGDKVAARQAAIEAGVQVVPGTPGPITTADEAVEFAKQYGTPIILKAAYGGGGRGIRRVDKLEEVEEAFRRSYSEAQAAFGDGSLFVEKFVERPRHIEVQLLGDHHGNIVHLYERDCSVQRRHQKVVEIAPAPALPEGVREKILADALRLARHVGYQNAGTVEFLVDQKGNYYFIEVNARLQVEHTVTEEITGVDLVQAQIRIAEGKSLDDLKLSQETIQTTGSAIQCRVTTEDPAKGFQPDSGRIEVFRSGEGMGIRLDSASAFAGSVISPHYDSLMVKVIASARNHPNAAAKMIRALKKFRIRGVKTNIPFLLNVLRQPSFLDASVDTYFIDEHPELFQFKPSQNRAQKLLNYLGEVKVNGPTTPLATDLKPAVVSPPIPYIPAGAKPPTGLRDVLVQRGPTEFAKEVRSRPGCMITDTTFRDAHQSLLATRVRTYDMAAISPFVAQSFNGLFSLENWGGATFDVSMRFLHECPWERLQTLRKLIPNIPFQCLLRGANAMGYSNYPDNVIYKFCELAVKNGMDVFRVFDSLNYLPNLLVGMEAVGKAGGVVEAAIAYTGDVTDKSRDKYDLKYYLNLADQLVKAQAHILSIKDMAGVLKPEAAKLLIGALRDKFPDIPIHVHTHDTSGAGVAAMLECAKAGADVVDAAVDSMSGMTSQPSMGAIVASLQGTKHDTGLSLDDISKYSAYWESTRQLYAPFECATTMKSGNADVYKHEIPGGQYTNLQFQAFSLGLGPQFDEVKRMYREANLVLGDIIKVTPSSKIVGDLAQFMVQNNLTRETLVDRADDLSFPKSVVDFMQGNVGQPPYGFPEPLRTKVLRGKPKVDGRPGENAKPVDLDAVKVELEEKHGRTLSEEDVMSYSMFPTVFDEFETFRQQYGPVDKLPTRLFLTGLEIAEEVDVEIESGKTLAIQLLAEGKLNKRGEREVFFDLNGQMRSIFVVDKEASKEIVTRPRALPGVRGHIGAPMPGDVLELKIKEGDKVTKKQPLFVLSAMKMEMVIDSPIAGTVKAIHAPQGTKCSAGDLVVEVEP

Target ORF information:

RefSeq Version	NM_001269447.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269447.2

ORF Insert Sequence:

ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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pyc-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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