ORF » Species Summary » Caenorhabditis elegans » pyc-1 cDNA ORF clone

pyc-1 cDNA ORF clone, Caenorhabditis elegans

The following pyc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pyc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa347798 NM_001269448.2
Latest version!		 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCa345711 NM_001269447.2
Latest version!		 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OCa347798 NM_001269448.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa345711 NM_001269447.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pyc-1 ( NM_001269448.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256377.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.
    Price & Availability↑
    CloneID OCa347798
    Clone ID Related Accession (Same CDS sequence) NM_001269448.2 , NM_001269448.1
    Accession Version NM_001269448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Pyruvate carboxylase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269448.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
    ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
    CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
    TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
    CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
    GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
    CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
    ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
    TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
    GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
    CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
    CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
    ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
    ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
    CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
    CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
    GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
    ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
    AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
    GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
    AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
    CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
    GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
    CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
    ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
    GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
    ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
    GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
    AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
    AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
    CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001256377.1
    CDS1..1851
    Translation

    Target ORF information:

    RefSeq Version NM_001269448.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001269448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
    ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
    CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
    TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
    CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
    GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
    CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
    ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
    TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
    GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
    CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
    CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
    ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
    ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
    CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
    CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
    GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
    ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
    AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
    GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
    AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
    CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
    GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
    CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
    ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
    GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
    ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
    GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
    AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
    AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
    CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pyc-1 ( NM_001269448.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256377.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.
    Price & Availability↑
    CloneID OCa347798
    Clone ID Related Accession (Same CDS sequence) NM_001269448.2 , NM_001269448.1
    Accession Version NM_001269448.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Pyruvate carboxylase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
    ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
    CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
    TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
    CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
    GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
    CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
    ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
    TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
    GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
    CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
    CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
    ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
    ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
    CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
    CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
    GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
    ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
    AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
    GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
    AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
    CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
    GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
    CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
    ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
    GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
    ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
    GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
    AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
    AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
    CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001256377.1
    CDS1..1851
    Translation

    Target ORF information:

    RefSeq Version NM_001269448.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001269448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATCACTG ATACTACATT CAGAGATGCA CATCAATCTC TTCTTGCAAC ACGAGTACGT 
    ACCTATGATA TGGCTGCTAT TTCACCTTTT GTTGCCCAAT CATTCAATGG TCTCTTCTCA 
    CTTGAAAACT GGGGAGGAGC TACTTTCGAT GTTTCCATGC GCTTCCTCCA CGAGTGTCCA 
    TGGGAACGTC TTCAAACTCT TCGCAAACTG ATTCCAAATA TTCCATTCCA ATGTCTTCTG 
    CGAGGTGCCA ATGCTATGGG ATATTCAAAT TATCCTGATA ACGTTATTTA CAAGTTCTGC 
    GAGTTGGCGG TGAAAAATGG AATGGATGTG TTCCGTGTAT TTGATTCACT GAACTATCTT 
    CCAAATCTTC TCGTCGGAAT GGAAGCCGTT GGAAAGGCCG GTGGAGTTGT TGAAGCTGCT 
    ATCGCATACA CTGGAGATGT TACCGATAAG TCTCGTGACA AATACGACCT CAAATACTAT 
    TTGAACTTGG CTGATCAACT TGTCAAAGCT CAAGCGCATA TTCTTTCTAT CAAGGATATG 
    GCTGGAGTTC TGAAACCGGA AGCTGCCAAG TTGTTGATTG GCGCTCTCCG AGACAAGTTC 
    CCAGATATTC CAATTCACGT TCATACCCAC GATACATCTG GAGCTGGAGT TGCTGCTATG 
    CTTGAGTGTG CCAAAGCTGG AGCTGATGTG GTCGATGCTG CAGTTGATAG CATGAGTGGA 
    ATGACTTCTC AGCCTTCAAT GGGAGCTATT GTAGCTAGTC TTCAAGGAAC TAAACATGAT 
    ACTGGTCTTT CTCTTGATGA CATTTCAAAA TATTCTGCAT ACTGGGAGTC AACTCGTCAA 
    CTCTATGCTC CATTCGAATG TGCCACCACC ATGAAAAGTG GAAATGCCGA TGTTTACAAG 
    CACGAAATTC CAGGAGGACA GTACACGAAC TTGCAATTCC AAGCATTCTC TCTCGGACTT 
    GGGCCACAGT TCGATGAAGT CAAGCGTATG TATCGTGAAG CTAATCTTGT TCTTGGCGAC 
    ATAATTAAAG TTACGCCATC ATCCAAGATT GTCGGTGACT TGGCTCAGTT TATGGTGCAA 
    AACAACTTGA CTCGCGAAAC TCTTGTCGAC CGTGCCGATG ATCTATCCTT CCCAAAAAGT 
    GTCGTTGATT TCATGCAAGG AAATGTCGGA CAACCTCCAT ATGGATTCCC TGAGCCATTG 
    AGAACTAAAG TCCTTCGTGG AAAGCCAAAA GTCGATGGAA GGCCAGGAGA AAATGCTAAG 
    CCAGTTGATC TAGATGCTGT GAAGGTCGAA CTTGAAGAGA AACATGGACG TACTTTGTCT 
    GAAGAAGATG TGATGTCATA TTCGATGTTC CCAACGGTTT TCGATGAGTT TGAGACTTTC 
    CGTCAACAAT ATGGACCAGT TGATAAGCTG CCAACGAGAC TCTTCTTGAC TGGATTGGAG 
    ATTGCGGAAG AAGTTGATGT TGAAATCGAG AGTGGAAAAA CTCTTGCAAT TCAATTGCTC 
    GCTGAAGGAA AACTAAACAA GCGTGGAGAA CGCGAAGTCT TCTTTGATCT CAATGGACAA 
    ATGAGAAGTA TCTTCGTGGT TGACAAGGAA GCTTCCAAGG AAATTGTAAC TCGCCCAAGA 
    GCTCTCCCAG GTGTCCGTGG ACATATTGGA GCACCAATGC CAGGAGATGT TTTGGAGCTG 
    AAAATCAAGG AAGGCGACAA GGTTACCAAG AAGCAACCCC TATTTGTGCT TTCCGCCATG 
    AAAATGGAGA TGGTCATCGA CTCTCCAATT GCTGGAACCG TCAAGGCTAT CCATGCACCA 
    CAAGGAACCA AATGTAGTGC CGGAGACTTG GTAGTTGAAG TTGAACCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pyc-1 ( NM_001269447.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256376.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.
    Price & Availability↑
    CloneID OCa345711
    Clone ID Related Accession (Same CDS sequence) NM_001269447.1 , NM_001269447.2
    Accession Version NM_001269447.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Pyruvate carboxylase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
    AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
    GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
    ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
    GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
    GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
    GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
    ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
    GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
    CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
    CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
    GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
    GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
    TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
    GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
    AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
    AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
    CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
    ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
    GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
    CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
    ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
    GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
    CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
    GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
    GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
    TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
    CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
    ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
    TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
    GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
    GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
    GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
    TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
    AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
    GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
    AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
    GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
    GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
    GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
    ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
    GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
    TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
    GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
    CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
    ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
    AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
    GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
    ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
    GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
    GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
    CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
    GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
    GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
    AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
    CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
    ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
    ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
    GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001256376.1
    CDS1..3528
    Translation

    Target ORF information:

    RefSeq Version NM_001269447.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001269447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
    AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
    GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
    ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
    GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
    GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
    GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
    ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
    GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
    CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
    CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
    GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
    GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
    TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
    GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
    AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
    AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
    CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
    ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
    GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
    CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
    ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
    GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
    CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
    GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
    GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
    TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
    CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
    ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
    TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
    GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
    GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
    GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
    TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
    AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
    GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
    AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
    GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
    GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
    GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
    ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
    GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
    TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
    GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
    CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
    ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
    AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
    GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
    ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
    GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
    GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
    CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
    GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
    GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
    AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
    CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
    ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
    ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
    GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pyc-1 ( NM_001269447.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256376.1 Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), mRNA.
    Price & Availability↑
    CloneID OCa345711
    Clone ID Related Accession (Same CDS sequence) NM_001269447.1 , NM_001269447.2
    Accession Version NM_001269447.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Pyruvate carboxylase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269447.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
    AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
    GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
    ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
    GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
    GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
    GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
    ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
    GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
    CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
    CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
    GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
    GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
    TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
    GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
    AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
    AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
    CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
    ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
    GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
    CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
    ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
    GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
    CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
    GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
    GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
    TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
    CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
    ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
    TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
    GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
    GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
    GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
    TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
    AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
    GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
    AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
    GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
    GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
    GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
    ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
    GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
    TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
    GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
    CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
    ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
    AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
    GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
    ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
    GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
    GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
    CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
    GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
    GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
    AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
    CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
    ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
    ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
    GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001256376.1
    CDS14..3541
    Translation

    Target ORF information:

    RefSeq Version NM_001269447.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Pyruvate carboxylase 1 (pyc-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001269447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGGTTCT CCCGCATCCC ACCGATCTTC GCTAATGTCG TCCGACAGAC ACATTATCGT 
    AACTATGCCA ATGGAGTTAT CAAACCAAGA GAATTCAACA AGGTTATGGT GGCCAATCGT 
    GGAGAAATTG CAATCCGTGT GTTCCGTGCC CTCACAGAAT TGAACAAAAC TTCTGTTGCG 
    ATTTACGCCG AGCAGGACAA AAACTCGATG CATCGTCTGA AAGCTGACGA AGCCTATCTT 
    GTAGGAAAAG GTCTTCCACC GGTTGCTGCT TATCTGACTA TTGACCAGAT TATAGAAACT 
    GCCCTGAAGC ACAACATTGA TGCTATTCAT CCCGGATATG GATTTCTGTC AGAGCGATCT 
    GACTTTGCAG CTGCTTGTCA AAATGCAGGA ATAGTGTTCA TTGGTCCTAG CCCCGACGTT 
    ATGGCTCGAA TGGGAGACAA AGTTGCTGCT CGTCAAGCTG CCATTGAAGC TGGTGTTCAA 
    GTCGTTCCAG GAACTCCTGG ACCAATTACA ACTGCTGATG AAGCTGTCGA ATTTGCAAAG 
    CAGTACGGTA CACCAATTAT TTTGAAGGCC GCTTATGGAG GAGGTGGTCG TGGAATTCGT 
    CGTGTTGATA AATTGGAAGA AGTTGAAGAG GCATTCCGTA GATCCTACTC GGAAGCTCAA 
    GCTGCGTTTG GAGACGGAAG TCTTTTCGTT GAAAAGTTTG TTGAGAGACC AAGACATATT 
    GAAGTTCAGC TGCTTGGAGA CCATCATGGA AATATTGTTC ATTTGTATGA GCGTGATTGT 
    TCAGTGCAAC GTCGTCATCA AAAGGTTGTT GAAATTGCTC CAGCGCCAGC TCTCCCAGAA 
    GGTGTTCGTG AGAAAATTTT GGCAGACGCT CTTCGACTTG CAAGACATGT TGGATACCAA 
    AATGCTGGTA CAGTCGAATT CCTGGTTGAT CAGAAGGGCA ACTACTATTT CATCGAAGTG 
    AATGCACGTC TTCAAGTCGA GCATACAGTA ACTGAAGAGA TCACTGGTGT GGATCTTGTC 
    CAAGCTCAAA TTCGTATCGC CGAAGGAAAA TCTCTGGATG ATCTGAAGCT TTCACAGGAA 
    ACTATTCAAA CTACTGGCTC AGCTATTCAA TGTCGTGTCA CAACTGAAGA TCCAGCTAAA 
    GGATTCCAGC CAGATTCCGG AAGAATTGAA GTTTTCCGAT CCGGAGAGGG AATGGGAATT 
    CGTCTTGATT CAGCATCTGC CTTCGCAGGA TCAGTCATTT CACCTCACTA CGATTCTTTG 
    ATGGTCAAAG TAATTGCATC GGCTAGAAAT CATCCGAACG CTGCCGCAAA AATGATTCGT 
    GCCCTCAAAA AGTTCCGTAT CCGAGGCGTA AAGACAAACA TCCCATTTCT GCTCAACGTT 
    CTTCGCCAGC CCAGCTTCCT TGATGCATCC GTCGATACGT ATTTCATTGA TGAGCATCCA 
    GAGTTGTTCC AATTCAAACC AAGCCAAAAC CGTGCTCAAA AGTTGTTGAA CTATTTGGGA 
    GAAGTTAAGG TGAACGGTCC AACTACTCCT CTTGCTACTG ACCTGAAACC AGCAGTTGTT 
    TCTCCTCCGA TCCCATACAT CCCAGCTGGA GCCAAACCGC CGACTGGACT TCGTGATGTA 
    CTTGTTCAAA GAGGACCAAC TGAGTTTGCA AAGGAAGTTC GCAGTAGACC AGGATGTATG 
    ATCACTGATA CTACATTCAG AGATGCACAT CAATCTCTTC TTGCAACACG AGTACGTACC 
    TATGATATGG CTGCTATTTC ACCTTTTGTT GCCCAATCAT TCAATGGTCT CTTCTCACTT 
    GAAAACTGGG GAGGAGCTAC TTTCGATGTT TCCATGCGCT TCCTCCACGA GTGTCCATGG 
    GAACGTCTTC AAACTCTTCG CAAACTGATT CCAAATATTC CATTCCAATG TCTTCTGCGA 
    GGTGCCAATG CTATGGGATA TTCAAATTAT CCTGATAACG TTATTTACAA GTTCTGCGAG 
    TTGGCGGTGA AAAATGGAAT GGATGTGTTC CGTGTATTTG ATTCACTGAA CTATCTTCCA 
    AATCTTCTCG TCGGAATGGA AGCCGTTGGA AAGGCCGGTG GAGTTGTTGA AGCTGCTATC 
    GCATACACTG GAGATGTTAC CGATAAGTCT CGTGACAAAT ACGACCTCAA ATACTATTTG 
    AACTTGGCTG ATCAACTTGT CAAAGCTCAA GCGCATATTC TTTCTATCAA GGATATGGCT 
    GGAGTTCTGA AACCGGAAGC TGCCAAGTTG TTGATTGGCG CTCTCCGAGA CAAGTTCCCA 
    GATATTCCAA TTCACGTTCA TACCCACGAT ACATCTGGAG CTGGAGTTGC TGCTATGCTT 
    GAGTGTGCCA AAGCTGGAGC TGATGTGGTC GATGCTGCAG TTGATAGCAT GAGTGGAATG 
    ACTTCTCAGC CTTCAATGGG AGCTATTGTA GCTAGTCTTC AAGGAACTAA ACATGATACT 
    GGTCTTTCTC TTGATGACAT TTCAAAATAT TCTGCATACT GGGAGTCAAC TCGTCAACTC 
    TATGCTCCAT TCGAATGTGC CACCACCATG AAAAGTGGAA ATGCCGATGT TTACAAGCAC 
    GAAATTCCAG GAGGACAGTA CACGAACTTG CAATTCCAAG CATTCTCTCT CGGACTTGGG 
    CCACAGTTCG ATGAAGTCAA GCGTATGTAT CGTGAAGCTA ATCTTGTTCT TGGCGACATA 
    ATTAAAGTTA CGCCATCATC CAAGATTGTC GGTGACTTGG CTCAGTTTAT GGTGCAAAAC 
    AACTTGACTC GCGAAACTCT TGTCGACCGT GCCGATGATC TATCCTTCCC AAAAAGTGTC 
    GTTGATTTCA TGCAAGGAAA TGTCGGACAA CCTCCATATG GATTCCCTGA GCCATTGAGA 
    ACTAAAGTCC TTCGTGGAAA GCCAAAAGTC GATGGAAGGC CAGGAGAAAA TGCTAAGCCA 
    GTTGATCTAG ATGCTGTGAA GGTCGAACTT GAAGAGAAAC ATGGACGTAC TTTGTCTGAA 
    GAAGATGTGA TGTCATATTC GATGTTCCCA ACGGTTTTCG ATGAGTTTGA GACTTTCCGT 
    CAACAATATG GACCAGTTGA TAAGCTGCCA ACGAGACTCT TCTTGACTGG ATTGGAGATT 
    GCGGAAGAAG TTGATGTTGA AATCGAGAGT GGAAAAACTC TTGCAATTCA ATTGCTCGCT 
    GAAGGAAAAC TAAACAAGCG TGGAGAACGC GAAGTCTTCT TTGATCTCAA TGGACAAATG 
    AGAAGTATCT TCGTGGTTGA CAAGGAAGCT TCCAAGGAAA TTGTAACTCG CCCAAGAGCT 
    CTCCCAGGTG TCCGTGGACA TATTGGAGCA CCAATGCCAG GAGATGTTTT GGAGCTGAAA 
    ATCAAGGAAG GCGACAAGGT TACCAAGAAG CAACCCCTAT TTGTGCTTTC CGCCATGAAA 
    ATGGAGATGG TCATCGACTC TCCAATTGCT GGAACCGTCA AGGCTATCCA TGCACCACAA 
    GGAACCAAAT GTAGTGCCGG AGACTTGGTA GTTGAAGTTG AACCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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