rol-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rol-3
Entrez Gene ID	179204
Full Name	Protein roller-3
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001269168.2	NP_001256097.1	Protein roller-3
NM_001269168.1	NP_001256097.1	Protein roller-3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ROL-3 is essential for epithelial development
Title: The ortholog of the human proto-oncogene ROS1 is required for epithelial development in C. elegans.

The following rol-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rol-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa347034	NM_001269168.2 Latest version!	Caenorhabditis elegans Protein roller-3 (rol-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OCa347034	NM_001269168.1	Caenorhabditis elegans Protein roller-3 (rol-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rol-3 ( NM_001269168.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256097.1 Caenorhabditis elegans Protein roller-3 (rol-3), mRNA.

Price & Availability↑

CloneID	OCa347034
Clone ID Related Accession (Same CDS sequence)	NM_001269168.2 , NM_001269168.1
Accession Version	NM_001269168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein roller-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269168.1.

ATGCTTTCTT TGCACCTCCG ATCACTTGCT ATTCTGTTTT TGTTGTTCTT CCTCCTCCAT 
GATGTTGTGA AATCAGCTAC CGTATTCTCC TCATCGTTAA AAACATGCCA AAGTCAATGT 
GAGGAGAGGA ATTTGGCATA TCCACTTGAT TCGGGTGAAG TTCACTGGAC GGGTCTTGCT 
GAATACAATT ACTCAAGTAG AATTTCATCG TGTAGACATG GATGTGAAGA TGTAGATGAA 
AGAGAATCTA AATGTGATGT GAAATGTTCA GAAGAAGGAA TTGTGTCGAA TGCCTGTAAA 
CAGGGATGTA GAGCAGTTCT TGTTTCGTTT CTAGCTCAGG CTCAAGCGCT TCTTATCCAA 
GTTCATGTAA ACATGGAGGT ACTCGAAACA TCAATGAAAC TGAAATGGGA GTTCCCTGAA 
ACTTTAGCAG AAGAGTTGAA AGAAATCGCA AATGCCGATA TTTTCTGGTT TTCCCAAACA 
AAACCACTTA ATGGAATTCT CGGATGGAGA TGGACATCGC TTCCTCAGAA TTCATTCAGA 
AACTCATCTC TGTCATCTGA AGTTCATGTT CCTTTTGAGC ACGGGGAACA CGTTGAAGTA 
CGATTAGCAC TGTCATATCG GAATCAGGTT CTTGTTTCTA GAACAACAAC ATATCATCTT 
CCATTATCAA AATCTGGAAC AACATTAGAA GTTATAGGAC AATTGCAACT TTCTGATGAT 
CGAGTAGCTG TTTGCTACAG GACTAATCAA CCAACGCCGA AATTCAAGTT GACAATTATG 
ACGCTGAATG ATAACACAAT CAACACAGAG GAATCAATTG CCAGGTGTCA TTTATTCTCA 
AATCTTCCAA GAGATAATTG TTGTAAAGCT TCAATTTCTG CAATTGATGA GCATGGTGCA 
ACGACGGCGT TTGTGGAAAT TAAACTGGAT TTTTATGTTA ATCAGGTTGA AATCGAACTT 
GTTTCTGTTG CATCTTCCCG TCTTATATTT TCAAACGGAA CTCATCTCCT AGAAAGTGAA 
ATTGATCAGT ATGCTCTTGG AGATTCGGCT ACTGTAATTC CCTTCCCATT ACCAACGGAT 
GACACAATTA CTGCTATTGC TGGAATCTCC GATACCACCA TTGCCATTGG AAGCTCAAAG 
GGATCGCTGT GGACATATCA AATGTCTGCC AATCAAACTG ACGAGGATCA ACCATCCTCT 
GTTATTCAAT TAAAAACAGT TGGAGAGATG GATACAAAAA TCAATCAAAT TGAAATCGAC 
CATATCCAGA GAACTCTTTA TGCAGTTCAA CACGATAAAG GAATTATTCG ATGCAAATTA 
AGAACGATGG AGGCAGAGGA GAGTCCGAAT TGTGTGCTAA TTGTTAATAA TGATGCTCTA 
AATGCACCGA AGGAGATAAC TTTGGATTCA GTCAATGGGC ATGTTTACAC ACTAAATGCC 
GACAATAAAG TTTATCGGAC TGAGATGGTT GCATTCAATG CAACAGGAAT AGAAACAGTT 
GCCAGTTTAC AATATCTTCA AGATATGTCT CCTTCCAATG GAATTTTCTT TGATGTTTCC 
AAGTTTCTTC TATATTCAGC TCTACAAAAT GGGTCCATGA TGACACTGAA TCCGGTCACG 
GATCATGTTC ACATTTTCAA GGACGTGGGC TACGCGGATA TTCAACATTT TAGAATAAAA 
AATGATCTAA TTTATTGGAT GAAAAAGAAG TGTGGGGAAA CCGACGCAGA CGAGAACTGC 
ATTTTTGCTG AAAACCTCCA ACGATCGGAA GAGGACATAC CTAACAAATT TACCTACTCC 
AGCTCCCTTA TGTCGTTTGG AGTTCTGGAA GAGATTTTGC TGAAGCCAAG AATAACCGCA 
GTCTCCACTA TAGCAATGCT CACATCAGAT AAGACAGCAA GAGTGTCATG GGATGAATCT 
AACACATTAC CGTTTCAAGC ACAAGGCAGC TCTTGGAGAA ACTTCACATA TTTTCTAAAA 
GTCACAGCTC CAGATATCAC TGATTTTTCA CCAATTGAAA TATATACCTC CAATACTGAA 
ATGAAAATTG ACGTTACACC TGGAAACATA TACAATGCAC AAGTACAAGT ATGTAGTGAT 
GACTTTTGTT CAATACCAAC TTCTACTTCA AATACAGCTC TCCCAGATTT AGGAGACGTT 
GTTCCCTTTG TTTTCACAAA AAGACAGGCA GATGATATTA TAAGTGTTGA TATTTTGGGA 
AATATTATAC CTGCCGATGA TTCTGTAAAA ATTGTGGAAA AACTTCAATA TCCTCATGTT 
CTGGATAACA CCACAAAAAC TGTGTATCTA GCTGGAGATC ATTCGATGGG AATATTCAAA 
AAATATTTGG ATGACACTGC TGGTTTACCA AAACCTTTCA AAGATGGACT TTTTGTCGAA 
ATGATGTCTA TAATGCCAGC GAGATCTATC ATTCTGATAG CTTCATCTTA TAAAATTACA 
TCTTACCGGC TGCCAACCAC TTTTGATTTC GAATACTTCT CATGCGAGGA ACCATTGGAA 
GCTTGTTCCG AAGTTATGGG AATATCAAGT GATGATACAA CCGGAATGGT TCATTTTCTA 
ACACAGGCAA GAAATGGAAC AATAACTCTC TGGGAATCAG ATCCAGAAAA TAGAACTCCA 
CGGGATATTG CTAGTGTTCC ATCAATTGTT CCATTTCGTC GATTTCTGAT TCTTCATGAC 
AAAATGATAC TTGTTACGAA AAACAATCAC ATTGTTCAAA CTGACAAAAA ACTGAAAGTG 
GTGAATGTTG CGACTGAATT GGAAAGAGTT GATCATATTC TACCATTACG ATATGCAACA 
ATATCTCACA AAATTGAGTT CAGTGATGAA ATCAAATTTA TTGATGGAAG TAAAACGAAT 
CTTCAGTGGA CATTATCCCC ACCATTAGAA GCTGGAACTG TACTCTTCAA AGTTTCAATT 
TTTCGAGAAA AAATGGGAGG CCAAGACCCA CCGATAATCA CGATTCAGAG TGAAACTAAC 
TTCACAATCC CGTCCGAAGT TCTGGAAGCT TGGAGTTCGG CGCAGAGATT TGACGTCTCT 
GTGCAAGCAA TGACACCGTG GGCCACAGCA GTACTCAACA GAACCGGACT AACAGCGCCA 
GTAAAACCAC CAACTTCTCC AACTCAATTG AGAATATTTG CAACTCAACA AAAAACTGTC 
GACGGTCCTA GAGCTCTCAT TTCATTTTTC TGGGGTCCTC CGAGTGAATG GAATGGAACA 
CCTTATCAGT ACATTGTCAA CTGCACAAAA GATGATGGAA GTTGGATTGG TGGACCTGTT 
ACTACTTCTC AATCACATTA TTCATTTGCT GTAAAATCTG GAAAAGTGTC TTGTCAGGCA 
GCAGCTGCAA ATGAGCCAAC GAATATTGGA AGTTTTTCAG AGTTGATCAC AATTGACAGT 
TCTGAACTTA AACCTCTTGT TAAACTTTTT GCAATTGATT CCACAAATTC TCTGATATCA 
ATTAATGATC TCGCTCATGA AGAACCGAGA AGAGAGACAC GGCAAGTTGC ACAACCAGTC 
AAGTTAGAGT ACCAAGCAAT GGCATTCATT GGGGAAGACT TGTACGCTGT CAGGAAAGAA 
GGAGAGTCTG CACAGCCAGT GTTGGTTCAG ATTGATACAA ATCATATTGA TAATACAGTA 
CATAAGGTGT CAATTGGTGG AGATGTAACA CGAATTGATG CAATGACTTC TGATTGGGTC 
GGAAATCGTT TAATATTTGT TGCAGGCACA AATGTATATC AGTTATCTCT TGAGCCATTT 
CTTTCAACTT CACTTCTAAA CCCGCATAAG TTGATTCAAC TAACATCCGC GACTGATGCA 
AAACAGCTTG CTTATGATCC TTTTATGAAC ACTGCCTACT TGCTTACCAA AAATGGATCA 
CTATTTGCAC TTGATATGAA CAAGAATACT GAAGCTAATC TGGCACTAAC TGTATCATGT 
TTGGCAAGTC AAACTGTTAC TTGGATGATG ACTGAATTTG CCTGGAATCG AGCTTCTTCA 
CCAAAAATTT ATGCGCTTAC GTGGAATGGA TTGATATATG TGGATCTTAC TGAAGACTCA 
CAGTGCAACG AAGTAAGAAT TGATTGGACC AAATTTGGAG ATAAAGGTCT CAAAGATATA 
TCATCATTTG CAATTGCTGA TAAACTATTT GCATTTGTCA CTTCTTCGGA AATGCTTATT 
TATGGGAAAG ACACTGTGAC ACCGATAACT ATTGCAAATC CACCACTGAA ACAAATACTC 
GCGGTCAGTC AAAGTAGTCA ACCATATCCG GAAAGATCTT GCTTCGAACT TCCGTCTTCA 
AAAGGGATTG TATTTTCAAT TGTGAACGAA GGGAAAACTG GATCATTCTT GGAAGTTACA 
AAGAGCTCAT CATCATCTTC CTGTCAAGAA GTTAGTATGC CACAGACCCA ATACGAAATA 
TATTTTACAC GGAAAAATAC TGATAAGGTG AAGCACGTAA GAAGCTTCTC TGACAGAATC 
CACGTGGAAA ATGGAATTCT TGACAAGGAA ACCGACTATG ATGTAACTGT AACTTGGCTC 
AATAGATATT CACCAGCAAG TGGAGTTTCT GCTTCAAAAT CTTTCCGAAC AGGGTTTGGG 
TACCCATCCG CTCCAAGAGA TCCTCATGCG ATTCCAGTTA CTCCAGATAC GGTTTATCTG 
TATTGGAATT TACCAGAGAC GCTGAATGCT CCAATATCAG AGATAAAATA TAAGATTTCA 
CAGCAAGCAG CAGGAATTTC TGTACCGACT TCAATTGCAG TCATTGCTCT TTCTGAAACT 
GTTTCTTCAA ACATTTCATC AGATACAACA TCTTGCTTAA TCAATCCATG TCGTGTTAAA 
ATTGCAAATC TTCGACCTTC AAATGAGTAC AAGTTTTGGG TAACAGCTAC TCATATCTCT 
CACCTGGATG CTGCTACAAT CCTGAAAGAT GACGATGCAG TGTCATCAGA AGCTGTTGCT 
AGAACTCTTG ATGTACCAGG AACATTGAGA CCTGATAATG TTACTGGATC TTCGTTACTT 
CTACGATGGA ATGGATTGGA ACCAGAGCAT CGACCAAGCT CAATTGCAGT CCAGTACAGG 
GAGTCTGGTG GCGCTAATAA TGAATGGCAA TTTCCAATGA ATGTATCATT TGAACCAGAT 
GTAACAACTG AACTTGTACC AATTACCAAC CTTTTGTCAG CTACTTCATA TGACTATCGA 
TTTGTGGCAA CCTACACAGG AACCTATACA ATTGATGGAA AAGTACTAGC ATTCAAAGAA 
GACTACCTCC AATTGCCGCA ACAAGCTCGA ACAAAAGCTG GAGTACCTAC AGCTCCTCAA 
TTTGTGGAAG CAAAGCAAGA TGAAGAGGGA TGGATTGTAA CTTGGAAAGA ACCAATGAGT 
GATGGAGGAT CTCCAATTAC AAGTTATGCC GTTGAGACTA GAATTAATAA AACCGCAGAA 
TGGGAGATTG CTGAAAGAGG ATTAGATGGA TGGAAAACAT GGTGGAGACC CGGAAAATCA 
GATACAACAT CTTCAACTTC AACATCATCG ACCGAAGTTT CAGAGTTTCG AATTAGAGCA 
GCAAATATTG AAGGATTTGG AGCCTATGCT TATACAGAAG ATAAAAAAGA GGAGAAAGAA 
GAAGAACCAA GTTCAGTATT ACCGTATCTA CTGCTTCTTT CAATCATATT CCTTTTGGCA 
GCAATGATTT TAGTGGCTTG TTTTTGGTTG AAATCTCGCC GTCGTCAACA GCAGAAGAAA 
CGTGAAGCGG AAGACGAAAG AAATTGTATT CGACTTGACG TAGTTGCCAA TATGAATTTT 
TCCTCTTCAC ATCAATCTCT TCCTCCAGAA TATGAGAGTG AAATGAGAAA TCTTCCGGTA 
GTCAATTACA GTACTGTTAC TTTCAATGAT TACATTGATA CGTGTGCATA TGGAATTGTT 
CATAGTGGTA CTGCTGAAGA AGTACCAATG AGTTGGGAAA AAGACGTTAG AGTTGCTATT 
AAAAAGTTGA AGCCGAATCA TTCATTCCAA GAGAAAATGA TGTTTATGAA AGAAGCAATT 
CTTTTAAATA ATCTCGATCA CCCGAATATC GTCAAAGAAC TTGGTGTCTG CATTACACCT 
GGACAGGAAT TGATTTTATT GGAATACATG GAAGGTGGAA ACTTGTTGAA GTTTCTTCAA 
AAATCAACCC CAAATGAGTA CCAAAGTTCT GAATTATCTC CACGCGATCT TCTCTCAATT 
TCTGTGGATA TTGCCCGTGG CATGAACTAT CTTGAGAGAC TTCCTCATGT TCATAAGAAT 
CTTTCCGCGC GGAAGTGCCT TCTTGCCGGC CGGCCAGGTG TGACTAAACT TGAGATGGGA 
ATGTCGAAAG AGTTGAGCAA TGGACAAGTG AATCGTTCGG ATTTGGAAAA TATGGAAATT 
GTCCGTTGGA TGGCGCCGGA AGTTTTGAAA GATTTCCAGT TTTCATCAAA GAGTGATGTT 
TGGGCTTATG GTGTACTTCT CTACGAAGTT TTTTCATTCG GAGAAGTTCC ATATGGAGAT 
AAAGACAATA GAAGAATTAT GACTGATGTC AGAAATGGAA GTGTTCTACC AATACCTTCC 
TATTGTCCAT CGAAAAGGAT ATACAAAGTT ATAAAACAAT GTTTGACTTC TGATTCTACT 
AAAAGGGCAA ACTTTGCAAC AATTTTGAAG ATTTTCGAAA CATTTAGAGA CGATCAAAAG 
TGTCAGGATG ATAAACCAAT TCAATTCAAT GATGGAACAG ACAACACGAA TTTTAGTGCA 
TCACAAGATA GTACTTCAAG TCGTGAACCT CCATCTCCAT CCCATCGAAT GAGAGATTTC 
ATTGATACAA GAGATTTAGA ACCACCGTCC CCATCACATT TGAATCAAAG TTTTGGAGGA 
TTTGAACATC CATATGAAGG CGAACGCCCT GCAACAATGT GGAATGGAAG TGGCGCACGA 
AACTCTGCCA AAAATAGTAT TGGAAGATCA ATGAAAAAAG AGAAATTAAG AAATCCCATT 
CATTCGATGG ATGATCTAGT TGCTAGAAAT CAGAGACCAT TGAGCATTCA TTCAGAAGAT 
ACCGAATCTA CGGATTACGG AGCTTCATCA TCAATGTATT CTCCAGGATC TTCCAATCGA 
ATTTCAAGCC AAGTTGACCC TCCAATTGGT AGATTGAGCT CTGCTCCAGG AATTGGCATA 
GTCAATGATG CATTTGAGAG CAGTAATCCA TCGCTGAATT TGAGTAGAAG TTGGGCCGGG 
CTTTCAAGAG AAGTGAATCA GAATCCTGCT GGTGCTGGTA GTAGTGGAAC ACTTCCACAG 
CATACGAACT CTGCTGGACA TTTGAGACTC CCTGGGGTCC AAGTTGGAGC AGGTGGCGGC 
CGTGTCTACA GAAATGCCTC TGGCGGTGGT GGACCTTCAA ATCGAGGCCG CATCAGTCAA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256097.1
CDS	59..7504
Translation	MLSLHLRSLAILFLLFFLLHDVVKSATVFSSSLKTCQSQCEERNLAYPLDSGEVHWTGLAEYNYSSRISSCRHGCEDVDERESKCDVKCSEEGIVSNACKQGCRAVLVSFLAQAQALLIQVHVNMEVLETSMKLKWEFPETLAEELKEIANADIFWFSQTKPLNGILGWRWTSLPQNSFRNSSLSSEVHVPFEHGEHVEVRLALSYRNQVLVSRTTTYHLPLSKSGTTLEVIGQLQLSDDRVAVCYRTNQPTPKFKLTIMTLNDNTINTEESIARCHLFSNLPRDNCCKASISAIDEHGATTAFVEIKLDFYVNQVEIELVSVASSRLIFSNGTHLLESEIDQYALGDSATVIPFPLPTDDTITAIAGISDTTIAIGSSKGSLWTYQMSANQTDEDQPSSVIQLKTVGEMDTKINQIEIDHIQRTLYAVQHDKGIIRCKLRTMEAEESPNCVLIVNNDALNAPKEITLDSVNGHVYTLNADNKVYRTEMVAFNATGIETVASLQYLQDMSPSNGIFFDVSKFLLYSALQNGSMMTLNPVTDHVHIFKDVGYADIQHFRIKNDLIYWMKKKCGETDADENCIFAENLQRSEEDIPNKFTYSSSLMSFGVLEEILLKPRITAVSTIAMLTSDKTARVSWDESNTLPFQAQGSSWRNFTYFLKVTAPDITDFSPIEIYTSNTEMKIDVTPGNIYNAQVQVCSDDFCSIPTSTSNTALPDLGDVVPFVFTKRQADDIISVDILGNIIPADDSVKIVEKLQYPHVLDNTTKTVYLAGDHSMGIFKKYLDDTAGLPKPFKDGLFVEMMSIMPARSIILIASSYKITSYRLPTTFDFEYFSCEEPLEACSEVMGISSDDTTGMVHFLTQARNGTITLWESDPENRTPRDIASVPSIVPFRRFLILHDKMILVTKNNHIVQTDKKLKVVNVATELERVDHILPLRYATISHKIEFSDEIKFIDGSKTNLQWTLSPPLEAGTVLFKVSIFREKMGGQDPPIITIQSETNFTIPSEVLEAWSSAQRFDVSVQAMTPWATAVLNRTGLTAPVKPPTSPTQLRIFATQQKTVDGPRALISFFWGPPSEWNGTPYQYIVNCTKDDGSWIGGPVTTSQSHYSFAVKSGKVSCQAAAANEPTNIGSFSELITIDSSELKPLVKLFAIDSTNSLISINDLAHEEPRRETRQVAQPVKLEYQAMAFIGEDLYAVRKEGESAQPVLVQIDTNHIDNTVHKVSIGGDVTRIDAMTSDWVGNRLIFVAGTNVYQLSLEPFLSTSLLNPHKLIQLTSATDAKQLAYDPFMNTAYLLTKNGSLFALDMNKNTEANLALTVSCLASQTVTWMMTEFAWNRASSPKIYALTWNGLIYVDLTEDSQCNEVRIDWTKFGDKGLKDISSFAIADKLFAFVTSSEMLIYGKDTVTPITIANPPLKQILAVSQSSQPYPERSCFELPSSKGIVFSIVNEGKTGSFLEVTKSSSSSSCQEVSMPQTQYEIYFTRKNTDKVKHVRSFSDRIHVENGILDKETDYDVTVTWLNRYSPASGVSASKSFRTGFGYPSAPRDPHAIPVTPDTVYLYWNLPETLNAPISEIKYKISQQAAGISVPTSIAVIALSETVSSNISSDTTSCLINPCRVKIANLRPSNEYKFWVTATHISHLDAATILKDDDAVSSEAVARTLDVPGTLRPDNVTGSSLLLRWNGLEPEHRPSSIAVQYRESGGANNEWQFPMNVSFEPDVTTELVPITNLLSATSYDYRFVATYTGTYTIDGKVLAFKEDYLQLPQQARTKAGVPTAPQFVEAKQDEEGWIVTWKEPMSDGGSPITSYAVETRINKTAEWEIAERGLDGWKTWWRPGKSDTTSSTSTSSTEVSEFRIRAANIEGFGAYAYTEDKKEEKEEEPSSVLPYLLLLSIIFLLAAMILVACFWLKSRRRQQQKKREAEDERNCIRLDVVANMNFSSSHQSLPPEYESEMRNLPVVNYSTVTFNDYIDTCAYGIVHSGTAEEVPMSWEKDVRVAIKKLKPNHSFQEKMMFMKEAILLNNLDHPNIVKELGVCITPGQELILLEYMEGGNLLKFLQKSTPNEYQSSELSPRDLLSISVDIARGMNYLERLPHVHKNLSARKCLLAGRPGVTKLEMGMSKELSNGQVNRSDLENMEIVRWMAPEVLKDFQFSSKSDVWAYGVLLYEVFSFGEVPYGDKDNRRIMTDVRNGSVLPIPSYCPSKRIYKVIKQCLTSDSTKRANFATILKIFETFRDDQKCQDDKPIQFNDGTDNTNFSASQDSTSSREPPSPSHRMRDFIDTRDLEPPSPSHLNQSFGGFEHPYEGERPATMWNGSGARNSAKNSIGRSMKKEKLRNPIHSMDDLVARNQRPLSIHSEDTESTDYGASSSMYSPGSSNRISSQVDPPIGRLSSAPGIGIVNDAFESSNPSLNLSRSWAGLSREVNQNPAGAGSSGTLPQHTNSAGHLRLPGVQVGAGGGRVYRNASGGGGPSNRGRISQV

Target ORF information:

RefSeq Version	NM_001269168.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein roller-3 (rol-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269168.2

ORF Insert Sequence:

ATGCTTTCTT TGCACCTCCG ATCACTTGCT ATTCTGTTTT TGTTGTTCTT CCTCCTCCAT 
GATGTTGTGA AATCAGCTAC CGTATTCTCC TCATCGTTAA AAACATGCCA AAGTCAATGT 
GAGGAGAGGA ATTTGGCATA TCCACTTGAT TCGGGTGAAG TTCACTGGAC GGGTCTTGCT 
GAATACAATT ACTCAAGTAG AATTTCATCG TGTAGACATG GATGTGAAGA TGTAGATGAA 
AGAGAATCTA AATGTGATGT GAAATGTTCA GAAGAAGGAA TTGTGTCGAA TGCCTGTAAA 
CAGGGATGTA GAGCAGTTCT TGTTTCGTTT CTAGCTCAGG CTCAAGCGCT TCTTATCCAA 
GTTCATGTAA ACATGGAGGT ACTCGAAACA TCAATGAAAC TGAAATGGGA GTTCCCTGAA 
ACTTTAGCAG AAGAGTTGAA AGAAATCGCA AATGCCGATA TTTTCTGGTT TTCCCAAACA 
AAACCACTTA ATGGAATTCT CGGATGGAGA TGGACATCGC TTCCTCAGAA TTCATTCAGA 
AACTCATCTC TGTCATCTGA AGTTCATGTT CCTTTTGAGC ACGGGGAACA CGTTGAAGTA 
CGATTAGCAC TGTCATATCG GAATCAGGTT CTTGTTTCTA GAACAACAAC ATATCATCTT 
CCATTATCAA AATCTGGAAC AACATTAGAA GTTATAGGAC AATTGCAACT TTCTGATGAT 
CGAGTAGCTG TTTGCTACAG GACTAATCAA CCAACGCCGA AATTCAAGTT GACAATTATG 
ACGCTGAATG ATAACACAAT CAACACAGAG GAATCAATTG CCAGGTGTCA TTTATTCTCA 
AATCTTCCAA GAGATAATTG TTGTAAAGCT TCAATTTCTG CAATTGATGA GCATGGTGCA 
ACGACGGCGT TTGTGGAAAT TAAACTGGAT TTTTATGTTA ATCAGGTTGA AATCGAACTT 
GTTTCTGTTG CATCTTCCCG TCTTATATTT TCAAACGGAA CTCATCTCCT AGAAAGTGAA 
ATTGATCAGT ATGCTCTTGG AGATTCGGCT ACTGTAATTC CCTTCCCATT ACCAACGGAT 
GACACAATTA CTGCTATTGC TGGAATCTCC GATACCACCA TTGCCATTGG AAGCTCAAAG 
GGATCGCTGT GGACATATCA AATGTCTGCC AATCAAACTG ACGAGGATCA ACCATCCTCT 
GTTATTCAAT TAAAAACAGT TGGAGAGATG GATACAAAAA TCAATCAAAT TGAAATCGAC 
CATATCCAGA GAACTCTTTA TGCAGTTCAA CACGATAAAG GAATTATTCG ATGCAAATTA 
AGAACGATGG AGGCAGAGGA GAGTCCGAAT TGTGTGCTAA TTGTTAATAA TGATGCTCTA 
AATGCACCGA AGGAGATAAC TTTGGATTCA GTCAATGGGC ATGTTTACAC ACTAAATGCC 
GACAATAAAG TTTATCGGAC TGAGATGGTT GCATTCAATG CAACAGGAAT AGAAACAGTT 
GCCAGTTTAC AATATCTTCA AGATATGTCT CCTTCCAATG GAATTTTCTT TGATGTTTCC 
AAGTTTCTTC TATATTCAGC TCTACAAAAT GGGTCCATGA TGACACTGAA TCCGGTCACG 
GATCATGTTC ACATTTTCAA GGACGTGGGC TACGCGGATA TTCAACATTT TAGAATAAAA 
AATGATCTAA TTTATTGGAT GAAAAAGAAG TGTGGGGAAA CCGACGCAGA CGAGAACTGC 
ATTTTTGCTG AAAACCTCCA ACGATCGGAA GAGGACATAC CTAACAAATT TACCTACTCC 
AGCTCCCTTA TGTCGTTTGG AGTTCTGGAA GAGATTTTGC TGAAGCCAAG AATAACCGCA 
GTCTCCACTA TAGCAATGCT CACATCAGAT AAGACAGCAA GAGTGTCATG GGATGAATCT 
AACACATTAC CGTTTCAAGC ACAAGGCAGC TCTTGGAGAA ACTTCACATA TTTTCTAAAA 
GTCACAGCTC CAGATATCAC TGATTTTTCA CCAATTGAAA TATATACCTC CAATACTGAA 
ATGAAAATTG ACGTTACACC TGGAAACATA TACAATGCAC AAGTACAAGT ATGTAGTGAT 
GACTTTTGTT CAATACCAAC TTCTACTTCA AATACAGCTC TCCCAGATTT AGGAGACGTT 
GTTCCCTTTG TTTTCACAAA AAGACAGGCA GATGATATTA TAAGTGTTGA TATTTTGGGA 
AATATTATAC CTGCCGATGA TTCTGTAAAA ATTGTGGAAA AACTTCAATA TCCTCATGTT 
CTGGATAACA CCACAAAAAC TGTGTATCTA GCTGGAGATC ATTCGATGGG AATATTCAAA 
AAATATTTGG ATGACACTGC TGGTTTACCA AAACCTTTCA AAGATGGACT TTTTGTCGAA 
ATGATGTCTA TAATGCCAGC GAGATCTATC ATTCTGATAG CTTCATCTTA TAAAATTACA 
TCTTACCGGC TGCCAACCAC TTTTGATTTC GAATACTTCT CATGCGAGGA ACCATTGGAA 
GCTTGTTCCG AAGTTATGGG AATATCAAGT GATGATACAA CCGGAATGGT TCATTTTCTA 
ACACAGGCAA GAAATGGAAC AATAACTCTC TGGGAATCAG ATCCAGAAAA TAGAACTCCA 
CGGGATATTG CTAGTGTTCC ATCAATTGTT CCATTTCGTC GATTTCTGAT TCTTCATGAC 
AAAATGATAC TTGTTACGAA AAACAATCAC ATTGTTCAAA CTGACAAAAA ACTGAAAGTG 
GTGAATGTTG CGACTGAATT GGAAAGAGTT GATCATATTC TACCATTACG ATATGCAACA 
ATATCTCACA AAATTGAGTT CAGTGATGAA ATCAAATTTA TTGATGGAAG TAAAACGAAT 
CTTCAGTGGA CATTATCCCC ACCATTAGAA GCTGGAACTG TACTCTTCAA AGTTTCAATT 
TTTCGAGAAA AAATGGGAGG CCAAGACCCA CCGATAATCA CGATTCAGAG TGAAACTAAC 
TTCACAATCC CGTCCGAAGT TCTGGAAGCT TGGAGTTCGG CGCAGAGATT TGACGTCTCT 
GTGCAAGCAA TGACACCGTG GGCCACAGCA GTACTCAACA GAACCGGACT AACAGCGCCA 
GTAAAACCAC CAACTTCTCC AACTCAATTG AGAATATTTG CAACTCAACA AAAAACTGTC 
GACGGTCCTA GAGCTCTCAT TTCATTTTTC TGGGGTCCTC CGAGTGAATG GAATGGAACA 
CCTTATCAGT ACATTGTCAA CTGCACAAAA GATGATGGAA GTTGGATTGG TGGACCTGTT 
ACTACTTCTC AATCACATTA TTCATTTGCT GTAAAATCTG GAAAAGTGTC TTGTCAGGCA 
GCAGCTGCAA ATGAGCCAAC GAATATTGGA AGTTTTTCAG AGTTGATCAC AATTGACAGT 
TCTGAACTTA AACCTCTTGT TAAACTTTTT GCAATTGATT CCACAAATTC TCTGATATCA 
ATTAATGATC TCGCTCATGA AGAACCGAGA AGAGAGACAC GGCAAGTTGC ACAACCAGTC 
AAGTTAGAGT ACCAAGCAAT GGCATTCATT GGGGAAGACT TGTACGCTGT CAGGAAAGAA 
GGAGAGTCTG CACAGCCAGT GTTGGTTCAG ATTGATACAA ATCATATTGA TAATACAGTA 
CATAAGGTGT CAATTGGTGG AGATGTAACA CGAATTGATG CAATGACTTC TGATTGGGTC 
GGAAATCGTT TAATATTTGT TGCAGGCACA AATGTATATC AGTTATCTCT TGAGCCATTT 
CTTTCAACTT CACTTCTAAA CCCGCATAAG TTGATTCAAC TAACATCCGC GACTGATGCA 
AAACAGCTTG CTTATGATCC TTTTATGAAC ACTGCCTACT TGCTTACCAA AAATGGATCA 
CTATTTGCAC TTGATATGAA CAAGAATACT GAAGCTAATC TGGCACTAAC TGTATCATGT 
TTGGCAAGTC AAACTGTTAC TTGGATGATG ACTGAATTTG CCTGGAATCG AGCTTCTTCA 
CCAAAAATTT ATGCGCTTAC GTGGAATGGA TTGATATATG TGGATCTTAC TGAAGACTCA 
CAGTGCAACG AAGTAAGAAT TGATTGGACC AAATTTGGAG ATAAAGGTCT CAAAGATATA 
TCATCATTTG CAATTGCTGA TAAACTATTT GCATTTGTCA CTTCTTCGGA AATGCTTATT 
TATGGGAAAG ACACTGTGAC ACCGATAACT ATTGCAAATC CACCACTGAA ACAAATACTC 
GCGGTCAGTC AAAGTAGTCA ACCATATCCG GAAAGATCTT GCTTCGAACT TCCGTCTTCA 
AAAGGGATTG TATTTTCAAT TGTGAACGAA GGGAAAACTG GATCATTCTT GGAAGTTACA 
AAGAGCTCAT CATCATCTTC CTGTCAAGAA GTTAGTATGC CACAGACCCA ATACGAAATA 
TATTTTACAC GGAAAAATAC TGATAAGGTG AAGCACGTAA GAAGCTTCTC TGACAGAATC 
CACGTGGAAA ATGGAATTCT TGACAAGGAA ACCGACTATG ATGTAACTGT AACTTGGCTC 
AATAGATATT CACCAGCAAG TGGAGTTTCT GCTTCAAAAT CTTTCCGAAC AGGGTTTGGG 
TACCCATCCG CTCCAAGAGA TCCTCATGCG ATTCCAGTTA CTCCAGATAC GGTTTATCTG 
TATTGGAATT TACCAGAGAC GCTGAATGCT CCAATATCAG AGATAAAATA TAAGATTTCA 
CAGCAAGCAG CAGGAATTTC TGTACCGACT TCAATTGCAG TCATTGCTCT TTCTGAAACT 
GTTTCTTCAA ACATTTCATC AGATACAACA TCTTGCTTAA TCAATCCATG TCGTGTTAAA 
ATTGCAAATC TTCGACCTTC AAATGAGTAC AAGTTTTGGG TAACAGCTAC TCATATCTCT 
CACCTGGATG CTGCTACAAT CCTGAAAGAT GACGATGCAG TGTCATCAGA AGCTGTTGCT 
AGAACTCTTG ATGTACCAGG AACATTGAGA CCTGATAATG TTACTGGATC TTCGTTACTT 
CTACGATGGA ATGGATTGGA ACCAGAGCAT CGACCAAGCT CAATTGCAGT CCAGTACAGG 
GAGTCTGGTG GCGCTAATAA TGAATGGCAA TTTCCAATGA ATGTATCATT TGAACCAGAT 
GTAACAACTG AACTTGTACC AATTACCAAC CTTTTGTCAG CTACTTCATA TGACTATCGA 
TTTGTGGCAA CCTACACAGG AACCTATACA ATTGATGGAA AAGTACTAGC ATTCAAAGAA 
GACTACCTCC AATTGCCGCA ACAAGCTCGA ACAAAAGCTG GAGTACCTAC AGCTCCTCAA 
TTTGTGGAAG CAAAGCAAGA TGAAGAGGGA TGGATTGTAA CTTGGAAAGA ACCAATGAGT 
GATGGAGGAT CTCCAATTAC AAGTTATGCC GTTGAGACTA GAATTAATAA AACCGCAGAA 
TGGGAGATTG CTGAAAGAGG ATTAGATGGA TGGAAAACAT GGTGGAGACC CGGAAAATCA 
GATACAACAT CTTCAACTTC AACATCATCG ACCGAAGTTT CAGAGTTTCG AATTAGAGCA 
GCAAATATTG AAGGATTTGG AGCCTATGCT TATACAGAAG ATAAAAAAGA GGAGAAAGAA 
GAAGAACCAA GTTCAGTATT ACCGTATCTA CTGCTTCTTT CAATCATATT CCTTTTGGCA 
GCAATGATTT TAGTGGCTTG TTTTTGGTTG AAATCTCGCC GTCGTCAACA GCAGAAGAAA 
CGTGAAGCGG AAGACGAAAG AAATTGTATT CGACTTGACG TAGTTGCCAA TATGAATTTT 
TCCTCTTCAC ATCAATCTCT TCCTCCAGAA TATGAGAGTG AAATGAGAAA TCTTCCGGTA 
GTCAATTACA GTACTGTTAC TTTCAATGAT TACATTGATA CGTGTGCATA TGGAATTGTT 
CATAGTGGTA CTGCTGAAGA AGTACCAATG AGTTGGGAAA AAGACGTTAG AGTTGCTATT 
AAAAAGTTGA AGCCGAATCA TTCATTCCAA GAGAAAATGA TGTTTATGAA AGAAGCAATT 
CTTTTAAATA ATCTCGATCA CCCGAATATC GTCAAAGAAC TTGGTGTCTG CATTACACCT 
GGACAGGAAT TGATTTTATT GGAATACATG GAAGGTGGAA ACTTGTTGAA GTTTCTTCAA 
AAATCAACCC CAAATGAGTA CCAAAGTTCT GAATTATCTC CACGCGATCT TCTCTCAATT 
TCTGTGGATA TTGCCCGTGG CATGAACTAT CTTGAGAGAC TTCCTCATGT TCATAAGAAT 
CTTTCCGCGC GGAAGTGCCT TCTTGCCGGC CGGCCAGGTG TGACTAAACT TGAGATGGGA 
ATGTCGAAAG AGTTGAGCAA TGGACAAGTG AATCGTTCGG ATTTGGAAAA TATGGAAATT 
GTCCGTTGGA TGGCGCCGGA AGTTTTGAAA GATTTCCAGT TTTCATCAAA GAGTGATGTT 
TGGGCTTATG GTGTACTTCT CTACGAAGTT TTTTCATTCG GAGAAGTTCC ATATGGAGAT 
AAAGACAATA GAAGAATTAT GACTGATGTC AGAAATGGAA GTGTTCTACC AATACCTTCC 
TATTGTCCAT CGAAAAGGAT ATACAAAGTT ATAAAACAAT GTTTGACTTC TGATTCTACT 
AAAAGGGCAA ACTTTGCAAC AATTTTGAAG ATTTTCGAAA CATTTAGAGA CGATCAAAAG 
TGTCAGGATG ATAAACCAAT TCAATTCAAT GATGGAACAG ACAACACGAA TTTTAGTGCA 
TCACAAGATA GTACTTCAAG TCGTGAACCT CCATCTCCAT CCCATCGAAT GAGAGATTTC 
ATTGATACAA GAGATTTAGA ACCACCGTCC CCATCACATT TGAATCAAAG TTTTGGAGGA 
TTTGAACATC CATATGAAGG CGAACGCCCT GCAACAATGT GGAATGGAAG TGGCGCACGA 
AACTCTGCCA AAAATAGTAT TGGAAGATCA ATGAAAAAAG AGAAATTAAG AAATCCCATT 
CATTCGATGG ATGATCTAGT TGCTAGAAAT CAGAGACCAT TGAGCATTCA TTCAGAAGAT 
ACCGAATCTA CGGATTACGG AGCTTCATCA TCAATGTATT CTCCAGGATC TTCCAATCGA 
ATTTCAAGCC AAGTTGACCC TCCAATTGGT AGATTGAGCT CTGCTCCAGG AATTGGCATA 
GTCAATGATG CATTTGAGAG CAGTAATCCA TCGCTGAATT TGAGTAGAAG TTGGGCCGGG 
CTTTCAAGAG AAGTGAATCA GAATCCTGCT GGTGCTGGTA GTAGTGGAAC ACTTCCACAG 
CATACGAACT CTGCTGGACA TTTGAGACTC CCTGGGGTCC AAGTTGGAGC AGGTGGCGGC 
CGTGTCTACA GAAATGCCTC TGGCGGTGGT GGACCTTCAA ATCGAGGCCG CATCAGTCAA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rol-3 ( NM_001269168.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256097.1 Caenorhabditis elegans Protein roller-3 (rol-3), partial mRNA.

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CloneID	OCa347034
Clone ID Related Accession (Same CDS sequence)	NM_001269168.2 , NM_001269168.1
Accession Version	NM_001269168.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein roller-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGCTTTCTT TGCACCTCCG ATCACTTGCT ATTCTGTTTT TGTTGTTCTT CCTCCTCCAT 
GATGTTGTGA AATCAGCTAC CGTATTCTCC TCATCGTTAA AAACATGCCA AAGTCAATGT 
GAGGAGAGGA ATTTGGCATA TCCACTTGAT TCGGGTGAAG TTCACTGGAC GGGTCTTGCT 
GAATACAATT ACTCAAGTAG AATTTCATCG TGTAGACATG GATGTGAAGA TGTAGATGAA 
AGAGAATCTA AATGTGATGT GAAATGTTCA GAAGAAGGAA TTGTGTCGAA TGCCTGTAAA 
CAGGGATGTA GAGCAGTTCT TGTTTCGTTT CTAGCTCAGG CTCAAGCGCT TCTTATCCAA 
GTTCATGTAA ACATGGAGGT ACTCGAAACA TCAATGAAAC TGAAATGGGA GTTCCCTGAA 
ACTTTAGCAG AAGAGTTGAA AGAAATCGCA AATGCCGATA TTTTCTGGTT TTCCCAAACA 
AAACCACTTA ATGGAATTCT CGGATGGAGA TGGACATCGC TTCCTCAGAA TTCATTCAGA 
AACTCATCTC TGTCATCTGA AGTTCATGTT CCTTTTGAGC ACGGGGAACA CGTTGAAGTA 
CGATTAGCAC TGTCATATCG GAATCAGGTT CTTGTTTCTA GAACAACAAC ATATCATCTT 
CCATTATCAA AATCTGGAAC AACATTAGAA GTTATAGGAC AATTGCAACT TTCTGATGAT 
CGAGTAGCTG TTTGCTACAG GACTAATCAA CCAACGCCGA AATTCAAGTT GACAATTATG 
ACGCTGAATG ATAACACAAT CAACACAGAG GAATCAATTG CCAGGTGTCA TTTATTCTCA 
AATCTTCCAA GAGATAATTG TTGTAAAGCT TCAATTTCTG CAATTGATGA GCATGGTGCA 
ACGACGGCGT TTGTGGAAAT TAAACTGGAT TTTTATGTTA ATCAGGTTGA AATCGAACTT 
GTTTCTGTTG CATCTTCCCG TCTTATATTT TCAAACGGAA CTCATCTCCT AGAAAGTGAA 
ATTGATCAGT ATGCTCTTGG AGATTCGGCT ACTGTAATTC CCTTCCCATT ACCAACGGAT 
GACACAATTA CTGCTATTGC TGGAATCTCC GATACCACCA TTGCCATTGG AAGCTCAAAG 
GGATCGCTGT GGACATATCA AATGTCTGCC AATCAAACTG ACGAGGATCA ACCATCCTCT 
GTTATTCAAT TAAAAACAGT TGGAGAGATG GATACAAAAA TCAATCAAAT TGAAATCGAC 
CATATCCAGA GAACTCTTTA TGCAGTTCAA CACGATAAAG GAATTATTCG ATGCAAATTA 
AGAACGATGG AGGCAGAGGA GAGTCCGAAT TGTGTGCTAA TTGTTAATAA TGATGCTCTA 
AATGCACCGA AGGAGATAAC TTTGGATTCA GTCAATGGGC ATGTTTACAC ACTAAATGCC 
GACAATAAAG TTTATCGGAC TGAGATGGTT GCATTCAATG CAACAGGAAT AGAAACAGTT 
GCCAGTTTAC AATATCTTCA AGATATGTCT CCTTCCAATG GAATTTTCTT TGATGTTTCC 
AAGTTTCTTC TATATTCAGC TCTACAAAAT GGGTCCATGA TGACACTGAA TCCGGTCACG 
GATCATGTTC ACATTTTCAA GGACGTGGGC TACGCGGATA TTCAACATTT TAGAATAAAA 
AATGATCTAA TTTATTGGAT GAAAAAGAAG TGTGGGGAAA CCGACGCAGA CGAGAACTGC 
ATTTTTGCTG AAAACCTCCA ACGATCGGAA GAGGACATAC CTAACAAATT TACCTACTCC 
AGCTCCCTTA TGTCGTTTGG AGTTCTGGAA GAGATTTTGC TGAAGCCAAG AATAACCGCA 
GTCTCCACTA TAGCAATGCT CACATCAGAT AAGACAGCAA GAGTGTCATG GGATGAATCT 
AACACATTAC CGTTTCAAGC ACAAGGCAGC TCTTGGAGAA ACTTCACATA TTTTCTAAAA 
GTCACAGCTC CAGATATCAC TGATTTTTCA CCAATTGAAA TATATACCTC CAATACTGAA 
ATGAAAATTG ACGTTACACC TGGAAACATA TACAATGCAC AAGTACAAGT ATGTAGTGAT 
GACTTTTGTT CAATACCAAC TTCTACTTCA AATACAGCTC TCCCAGATTT AGGAGACGTT 
GTTCCCTTTG TTTTCACAAA AAGACAGGCA GATGATATTA TAAGTGTTGA TATTTTGGGA 
AATATTATAC CTGCCGATGA TTCTGTAAAA ATTGTGGAAA AACTTCAATA TCCTCATGTT 
CTGGATAACA CCACAAAAAC TGTGTATCTA GCTGGAGATC ATTCGATGGG AATATTCAAA 
AAATATTTGG ATGACACTGC TGGTTTACCA AAACCTTTCA AAGATGGACT TTTTGTCGAA 
ATGATGTCTA TAATGCCAGC GAGATCTATC ATTCTGATAG CTTCATCTTA TAAAATTACA 
TCTTACCGGC TGCCAACCAC TTTTGATTTC GAATACTTCT CATGCGAGGA ACCATTGGAA 
GCTTGTTCCG AAGTTATGGG AATATCAAGT GATGATACAA CCGGAATGGT TCATTTTCTA 
ACACAGGCAA GAAATGGAAC AATAACTCTC TGGGAATCAG ATCCAGAAAA TAGAACTCCA 
CGGGATATTG CTAGTGTTCC ATCAATTGTT CCATTTCGTC GATTTCTGAT TCTTCATGAC 
AAAATGATAC TTGTTACGAA AAACAATCAC ATTGTTCAAA CTGACAAAAA ACTGAAAGTG 
GTGAATGTTG CGACTGAATT GGAAAGAGTT GATCATATTC TACCATTACG ATATGCAACA 
ATATCTCACA AAATTGAGTT CAGTGATGAA ATCAAATTTA TTGATGGAAG TAAAACGAAT 
CTTCAGTGGA CATTATCCCC ACCATTAGAA GCTGGAACTG TACTCTTCAA AGTTTCAATT 
TTTCGAGAAA AAATGGGAGG CCAAGACCCA CCGATAATCA CGATTCAGAG TGAAACTAAC 
TTCACAATCC CGTCCGAAGT TCTGGAAGCT TGGAGTTCGG CGCAGAGATT TGACGTCTCT 
GTGCAAGCAA TGACACCGTG GGCCACAGCA GTACTCAACA GAACCGGACT AACAGCGCCA 
GTAAAACCAC CAACTTCTCC AACTCAATTG AGAATATTTG CAACTCAACA AAAAACTGTC 
GACGGTCCTA GAGCTCTCAT TTCATTTTTC TGGGGTCCTC CGAGTGAATG GAATGGAACA 
CCTTATCAGT ACATTGTCAA CTGCACAAAA GATGATGGAA GTTGGATTGG TGGACCTGTT 
ACTACTTCTC AATCACATTA TTCATTTGCT GTAAAATCTG GAAAAGTGTC TTGTCAGGCA 
GCAGCTGCAA ATGAGCCAAC GAATATTGGA AGTTTTTCAG AGTTGATCAC AATTGACAGT 
TCTGAACTTA AACCTCTTGT TAAACTTTTT GCAATTGATT CCACAAATTC TCTGATATCA 
ATTAATGATC TCGCTCATGA AGAACCGAGA AGAGAGACAC GGCAAGTTGC ACAACCAGTC 
AAGTTAGAGT ACCAAGCAAT GGCATTCATT GGGGAAGACT TGTACGCTGT CAGGAAAGAA 
GGAGAGTCTG CACAGCCAGT GTTGGTTCAG ATTGATACAA ATCATATTGA TAATACAGTA 
CATAAGGTGT CAATTGGTGG AGATGTAACA CGAATTGATG CAATGACTTC TGATTGGGTC 
GGAAATCGTT TAATATTTGT TGCAGGCACA AATGTATATC AGTTATCTCT TGAGCCATTT 
CTTTCAACTT CACTTCTAAA CCCGCATAAG TTGATTCAAC TAACATCCGC GACTGATGCA 
AAACAGCTTG CTTATGATCC TTTTATGAAC ACTGCCTACT TGCTTACCAA AAATGGATCA 
CTATTTGCAC TTGATATGAA CAAGAATACT GAAGCTAATC TGGCACTAAC TGTATCATGT 
TTGGCAAGTC AAACTGTTAC TTGGATGATG ACTGAATTTG CCTGGAATCG AGCTTCTTCA 
CCAAAAATTT ATGCGCTTAC GTGGAATGGA TTGATATATG TGGATCTTAC TGAAGACTCA 
CAGTGCAACG AAGTAAGAAT TGATTGGACC AAATTTGGAG ATAAAGGTCT CAAAGATATA 
TCATCATTTG CAATTGCTGA TAAACTATTT GCATTTGTCA CTTCTTCGGA AATGCTTATT 
TATGGGAAAG ACACTGTGAC ACCGATAACT ATTGCAAATC CACCACTGAA ACAAATACTC 
GCGGTCAGTC AAAGTAGTCA ACCATATCCG GAAAGATCTT GCTTCGAACT TCCGTCTTCA 
AAAGGGATTG TATTTTCAAT TGTGAACGAA GGGAAAACTG GATCATTCTT GGAAGTTACA 
AAGAGCTCAT CATCATCTTC CTGTCAAGAA GTTAGTATGC CACAGACCCA ATACGAAATA 
TATTTTACAC GGAAAAATAC TGATAAGGTG AAGCACGTAA GAAGCTTCTC TGACAGAATC 
CACGTGGAAA ATGGAATTCT TGACAAGGAA ACCGACTATG ATGTAACTGT AACTTGGCTC 
AATAGATATT CACCAGCAAG TGGAGTTTCT GCTTCAAAAT CTTTCCGAAC AGGGTTTGGG 
TACCCATCCG CTCCAAGAGA TCCTCATGCG ATTCCAGTTA CTCCAGATAC GGTTTATCTG 
TATTGGAATT TACCAGAGAC GCTGAATGCT CCAATATCAG AGATAAAATA TAAGATTTCA 
CAGCAAGCAG CAGGAATTTC TGTACCGACT TCAATTGCAG TCATTGCTCT TTCTGAAACT 
GTTTCTTCAA ACATTTCATC AGATACAACA TCTTGCTTAA TCAATCCATG TCGTGTTAAA 
ATTGCAAATC TTCGACCTTC AAATGAGTAC AAGTTTTGGG TAACAGCTAC TCATATCTCT 
CACCTGGATG CTGCTACAAT CCTGAAAGAT GACGATGCAG TGTCATCAGA AGCTGTTGCT 
AGAACTCTTG ATGTACCAGG AACATTGAGA CCTGATAATG TTACTGGATC TTCGTTACTT 
CTACGATGGA ATGGATTGGA ACCAGAGCAT CGACCAAGCT CAATTGCAGT CCAGTACAGG 
GAGTCTGGTG GCGCTAATAA TGAATGGCAA TTTCCAATGA ATGTATCATT TGAACCAGAT 
GTAACAACTG AACTTGTACC AATTACCAAC CTTTTGTCAG CTACTTCATA TGACTATCGA 
TTTGTGGCAA CCTACACAGG AACCTATACA ATTGATGGAA AAGTACTAGC ATTCAAAGAA 
GACTACCTCC AATTGCCGCA ACAAGCTCGA ACAAAAGCTG GAGTACCTAC AGCTCCTCAA 
TTTGTGGAAG CAAAGCAAGA TGAAGAGGGA TGGATTGTAA CTTGGAAAGA ACCAATGAGT 
GATGGAGGAT CTCCAATTAC AAGTTATGCC GTTGAGACTA GAATTAATAA AACCGCAGAA 
TGGGAGATTG CTGAAAGAGG ATTAGATGGA TGGAAAACAT GGTGGAGACC CGGAAAATCA 
GATACAACAT CTTCAACTTC AACATCATCG ACCGAAGTTT CAGAGTTTCG AATTAGAGCA 
GCAAATATTG AAGGATTTGG AGCCTATGCT TATACAGAAG ATAAAAAAGA GGAGAAAGAA 
GAAGAACCAA GTTCAGTATT ACCGTATCTA CTGCTTCTTT CAATCATATT CCTTTTGGCA 
GCAATGATTT TAGTGGCTTG TTTTTGGTTG AAATCTCGCC GTCGTCAACA GCAGAAGAAA 
CGTGAAGCGG AAGACGAAAG AAATTGTATT CGACTTGACG TAGTTGCCAA TATGAATTTT 
TCCTCTTCAC ATCAATCTCT TCCTCCAGAA TATGAGAGTG AAATGAGAAA TCTTCCGGTA 
GTCAATTACA GTACTGTTAC TTTCAATGAT TACATTGATA CGTGTGCATA TGGAATTGTT 
CATAGTGGTA CTGCTGAAGA AGTACCAATG AGTTGGGAAA AAGACGTTAG AGTTGCTATT 
AAAAAGTTGA AGCCGAATCA TTCATTCCAA GAGAAAATGA TGTTTATGAA AGAAGCAATT 
CTTTTAAATA ATCTCGATCA CCCGAATATC GTCAAAGAAC TTGGTGTCTG CATTACACCT 
GGACAGGAAT TGATTTTATT GGAATACATG GAAGGTGGAA ACTTGTTGAA GTTTCTTCAA 
AAATCAACCC CAAATGAGTA CCAAAGTTCT GAATTATCTC CACGCGATCT TCTCTCAATT 
TCTGTGGATA TTGCCCGTGG CATGAACTAT CTTGAGAGAC TTCCTCATGT TCATAAGAAT 
CTTTCCGCGC GGAAGTGCCT TCTTGCCGGC CGGCCAGGTG TGACTAAACT TGAGATGGGA 
ATGTCGAAAG AGTTGAGCAA TGGACAAGTG AATCGTTCGG ATTTGGAAAA TATGGAAATT 
GTCCGTTGGA TGGCGCCGGA AGTTTTGAAA GATTTCCAGT TTTCATCAAA GAGTGATGTT 
TGGGCTTATG GTGTACTTCT CTACGAAGTT TTTTCATTCG GAGAAGTTCC ATATGGAGAT 
AAAGACAATA GAAGAATTAT GACTGATGTC AGAAATGGAA GTGTTCTACC AATACCTTCC 
TATTGTCCAT CGAAAAGGAT ATACAAAGTT ATAAAACAAT GTTTGACTTC TGATTCTACT 
AAAAGGGCAA ACTTTGCAAC AATTTTGAAG ATTTTCGAAA CATTTAGAGA CGATCAAAAG 
TGTCAGGATG ATAAACCAAT TCAATTCAAT GATGGAACAG ACAACACGAA TTTTAGTGCA 
TCACAAGATA GTACTTCAAG TCGTGAACCT CCATCTCCAT CCCATCGAAT GAGAGATTTC 
ATTGATACAA GAGATTTAGA ACCACCGTCC CCATCACATT TGAATCAAAG TTTTGGAGGA 
TTTGAACATC CATATGAAGG CGAACGCCCT GCAACAATGT GGAATGGAAG TGGCGCACGA 
AACTCTGCCA AAAATAGTAT TGGAAGATCA ATGAAAAAAG AGAAATTAAG AAATCCCATT 
CATTCGATGG ATGATCTAGT TGCTAGAAAT CAGAGACCAT TGAGCATTCA TTCAGAAGAT 
ACCGAATCTA CGGATTACGG AGCTTCATCA TCAATGTATT CTCCAGGATC TTCCAATCGA 
ATTTCAAGCC AAGTTGACCC TCCAATTGGT AGATTGAGCT CTGCTCCAGG AATTGGCATA 
GTCAATGATG CATTTGAGAG CAGTAATCCA TCGCTGAATT TGAGTAGAAG TTGGGCCGGG 
CTTTCAAGAG AAGTGAATCA GAATCCTGCT GGTGCTGGTA GTAGTGGAAC ACTTCCACAG 
CATACGAACT CTGCTGGACA TTTGAGACTC CCTGGGGTCC AAGTTGGAGC AGGTGGCGGC 
CGTGTCTACA GAAATGCCTC TGGCGGTGGT GGACCTTCAA ATCGAGGCCG CATCAGTCAA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256097.1
CDS	1..7446
Translation	MLSLHLRSLAILFLLFFLLHDVVKSATVFSSSLKTCQSQCEERNLAYPLDSGEVHWTGLAEYNYSSRISSCRHGCEDVDERESKCDVKCSEEGIVSNACKQGCRAVLVSFLAQAQALLIQVHVNMEVLETSMKLKWEFPETLAEELKEIANADIFWFSQTKPLNGILGWRWTSLPQNSFRNSSLSSEVHVPFEHGEHVEVRLALSYRNQVLVSRTTTYHLPLSKSGTTLEVIGQLQLSDDRVAVCYRTNQPTPKFKLTIMTLNDNTINTEESIARCHLFSNLPRDNCCKASISAIDEHGATTAFVEIKLDFYVNQVEIELVSVASSRLIFSNGTHLLESEIDQYALGDSATVIPFPLPTDDTITAIAGISDTTIAIGSSKGSLWTYQMSANQTDEDQPSSVIQLKTVGEMDTKINQIEIDHIQRTLYAVQHDKGIIRCKLRTMEAEESPNCVLIVNNDALNAPKEITLDSVNGHVYTLNADNKVYRTEMVAFNATGIETVASLQYLQDMSPSNGIFFDVSKFLLYSALQNGSMMTLNPVTDHVHIFKDVGYADIQHFRIKNDLIYWMKKKCGETDADENCIFAENLQRSEEDIPNKFTYSSSLMSFGVLEEILLKPRITAVSTIAMLTSDKTARVSWDESNTLPFQAQGSSWRNFTYFLKVTAPDITDFSPIEIYTSNTEMKIDVTPGNIYNAQVQVCSDDFCSIPTSTSNTALPDLGDVVPFVFTKRQADDIISVDILGNIIPADDSVKIVEKLQYPHVLDNTTKTVYLAGDHSMGIFKKYLDDTAGLPKPFKDGLFVEMMSIMPARSIILIASSYKITSYRLPTTFDFEYFSCEEPLEACSEVMGISSDDTTGMVHFLTQARNGTITLWESDPENRTPRDIASVPSIVPFRRFLILHDKMILVTKNNHIVQTDKKLKVVNVATELERVDHILPLRYATISHKIEFSDEIKFIDGSKTNLQWTLSPPLEAGTVLFKVSIFREKMGGQDPPIITIQSETNFTIPSEVLEAWSSAQRFDVSVQAMTPWATAVLNRTGLTAPVKPPTSPTQLRIFATQQKTVDGPRALISFFWGPPSEWNGTPYQYIVNCTKDDGSWIGGPVTTSQSHYSFAVKSGKVSCQAAAANEPTNIGSFSELITIDSSELKPLVKLFAIDSTNSLISINDLAHEEPRRETRQVAQPVKLEYQAMAFIGEDLYAVRKEGESAQPVLVQIDTNHIDNTVHKVSIGGDVTRIDAMTSDWVGNRLIFVAGTNVYQLSLEPFLSTSLLNPHKLIQLTSATDAKQLAYDPFMNTAYLLTKNGSLFALDMNKNTEANLALTVSCLASQTVTWMMTEFAWNRASSPKIYALTWNGLIYVDLTEDSQCNEVRIDWTKFGDKGLKDISSFAIADKLFAFVTSSEMLIYGKDTVTPITIANPPLKQILAVSQSSQPYPERSCFELPSSKGIVFSIVNEGKTGSFLEVTKSSSSSSCQEVSMPQTQYEIYFTRKNTDKVKHVRSFSDRIHVENGILDKETDYDVTVTWLNRYSPASGVSASKSFRTGFGYPSAPRDPHAIPVTPDTVYLYWNLPETLNAPISEIKYKISQQAAGISVPTSIAVIALSETVSSNISSDTTSCLINPCRVKIANLRPSNEYKFWVTATHISHLDAATILKDDDAVSSEAVARTLDVPGTLRPDNVTGSSLLLRWNGLEPEHRPSSIAVQYRESGGANNEWQFPMNVSFEPDVTTELVPITNLLSATSYDYRFVATYTGTYTIDGKVLAFKEDYLQLPQQARTKAGVPTAPQFVEAKQDEEGWIVTWKEPMSDGGSPITSYAVETRINKTAEWEIAERGLDGWKTWWRPGKSDTTSSTSTSSTEVSEFRIRAANIEGFGAYAYTEDKKEEKEEEPSSVLPYLLLLSIIFLLAAMILVACFWLKSRRRQQQKKREAEDERNCIRLDVVANMNFSSSHQSLPPEYESEMRNLPVVNYSTVTFNDYIDTCAYGIVHSGTAEEVPMSWEKDVRVAIKKLKPNHSFQEKMMFMKEAILLNNLDHPNIVKELGVCITPGQELILLEYMEGGNLLKFLQKSTPNEYQSSELSPRDLLSISVDIARGMNYLERLPHVHKNLSARKCLLAGRPGVTKLEMGMSKELSNGQVNRSDLENMEIVRWMAPEVLKDFQFSSKSDVWAYGVLLYEVFSFGEVPYGDKDNRRIMTDVRNGSVLPIPSYCPSKRIYKVIKQCLTSDSTKRANFATILKIFETFRDDQKCQDDKPIQFNDGTDNTNFSASQDSTSSREPPSPSHRMRDFIDTRDLEPPSPSHLNQSFGGFEHPYEGERPATMWNGSGARNSAKNSIGRSMKKEKLRNPIHSMDDLVARNQRPLSIHSEDTESTDYGASSSMYSPGSSNRISSQVDPPIGRLSSAPGIGIVNDAFESSNPSLNLSRSWAGLSREVNQNPAGAGSSGTLPQHTNSAGHLRLPGVQVGAGGGRVYRNASGGGGPSNRGRISQV

Target ORF information:

RefSeq Version	NM_001269168.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein roller-3 (rol-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269168.1

ORF Insert Sequence:

ATGCTTTCTT TGCACCTCCG ATCACTTGCT ATTCTGTTTT TGTTGTTCTT CCTCCTCCAT 
GATGTTGTGA AATCAGCTAC CGTATTCTCC TCATCGTTAA AAACATGCCA AAGTCAATGT 
GAGGAGAGGA ATTTGGCATA TCCACTTGAT TCGGGTGAAG TTCACTGGAC GGGTCTTGCT 
GAATACAATT ACTCAAGTAG AATTTCATCG TGTAGACATG GATGTGAAGA TGTAGATGAA 
AGAGAATCTA AATGTGATGT GAAATGTTCA GAAGAAGGAA TTGTGTCGAA TGCCTGTAAA 
CAGGGATGTA GAGCAGTTCT TGTTTCGTTT CTAGCTCAGG CTCAAGCGCT TCTTATCCAA 
GTTCATGTAA ACATGGAGGT ACTCGAAACA TCAATGAAAC TGAAATGGGA GTTCCCTGAA 
ACTTTAGCAG AAGAGTTGAA AGAAATCGCA AATGCCGATA TTTTCTGGTT TTCCCAAACA 
AAACCACTTA ATGGAATTCT CGGATGGAGA TGGACATCGC TTCCTCAGAA TTCATTCAGA 
AACTCATCTC TGTCATCTGA AGTTCATGTT CCTTTTGAGC ACGGGGAACA CGTTGAAGTA 
CGATTAGCAC TGTCATATCG GAATCAGGTT CTTGTTTCTA GAACAACAAC ATATCATCTT 
CCATTATCAA AATCTGGAAC AACATTAGAA GTTATAGGAC AATTGCAACT TTCTGATGAT 
CGAGTAGCTG TTTGCTACAG GACTAATCAA CCAACGCCGA AATTCAAGTT GACAATTATG 
ACGCTGAATG ATAACACAAT CAACACAGAG GAATCAATTG CCAGGTGTCA TTTATTCTCA 
AATCTTCCAA GAGATAATTG TTGTAAAGCT TCAATTTCTG CAATTGATGA GCATGGTGCA 
ACGACGGCGT TTGTGGAAAT TAAACTGGAT TTTTATGTTA ATCAGGTTGA AATCGAACTT 
GTTTCTGTTG CATCTTCCCG TCTTATATTT TCAAACGGAA CTCATCTCCT AGAAAGTGAA 
ATTGATCAGT ATGCTCTTGG AGATTCGGCT ACTGTAATTC CCTTCCCATT ACCAACGGAT 
GACACAATTA CTGCTATTGC TGGAATCTCC GATACCACCA TTGCCATTGG AAGCTCAAAG 
GGATCGCTGT GGACATATCA AATGTCTGCC AATCAAACTG ACGAGGATCA ACCATCCTCT 
GTTATTCAAT TAAAAACAGT TGGAGAGATG GATACAAAAA TCAATCAAAT TGAAATCGAC 
CATATCCAGA GAACTCTTTA TGCAGTTCAA CACGATAAAG GAATTATTCG ATGCAAATTA 
AGAACGATGG AGGCAGAGGA GAGTCCGAAT TGTGTGCTAA TTGTTAATAA TGATGCTCTA 
AATGCACCGA AGGAGATAAC TTTGGATTCA GTCAATGGGC ATGTTTACAC ACTAAATGCC 
GACAATAAAG TTTATCGGAC TGAGATGGTT GCATTCAATG CAACAGGAAT AGAAACAGTT 
GCCAGTTTAC AATATCTTCA AGATATGTCT CCTTCCAATG GAATTTTCTT TGATGTTTCC 
AAGTTTCTTC TATATTCAGC TCTACAAAAT GGGTCCATGA TGACACTGAA TCCGGTCACG 
GATCATGTTC ACATTTTCAA GGACGTGGGC TACGCGGATA TTCAACATTT TAGAATAAAA 
AATGATCTAA TTTATTGGAT GAAAAAGAAG TGTGGGGAAA CCGACGCAGA CGAGAACTGC 
ATTTTTGCTG AAAACCTCCA ACGATCGGAA GAGGACATAC CTAACAAATT TACCTACTCC 
AGCTCCCTTA TGTCGTTTGG AGTTCTGGAA GAGATTTTGC TGAAGCCAAG AATAACCGCA 
GTCTCCACTA TAGCAATGCT CACATCAGAT AAGACAGCAA GAGTGTCATG GGATGAATCT 
AACACATTAC CGTTTCAAGC ACAAGGCAGC TCTTGGAGAA ACTTCACATA TTTTCTAAAA 
GTCACAGCTC CAGATATCAC TGATTTTTCA CCAATTGAAA TATATACCTC CAATACTGAA 
ATGAAAATTG ACGTTACACC TGGAAACATA TACAATGCAC AAGTACAAGT ATGTAGTGAT 
GACTTTTGTT CAATACCAAC TTCTACTTCA AATACAGCTC TCCCAGATTT AGGAGACGTT 
GTTCCCTTTG TTTTCACAAA AAGACAGGCA GATGATATTA TAAGTGTTGA TATTTTGGGA 
AATATTATAC CTGCCGATGA TTCTGTAAAA ATTGTGGAAA AACTTCAATA TCCTCATGTT 
CTGGATAACA CCACAAAAAC TGTGTATCTA GCTGGAGATC ATTCGATGGG AATATTCAAA 
AAATATTTGG ATGACACTGC TGGTTTACCA AAACCTTTCA AAGATGGACT TTTTGTCGAA 
ATGATGTCTA TAATGCCAGC GAGATCTATC ATTCTGATAG CTTCATCTTA TAAAATTACA 
TCTTACCGGC TGCCAACCAC TTTTGATTTC GAATACTTCT CATGCGAGGA ACCATTGGAA 
GCTTGTTCCG AAGTTATGGG AATATCAAGT GATGATACAA CCGGAATGGT TCATTTTCTA 
ACACAGGCAA GAAATGGAAC AATAACTCTC TGGGAATCAG ATCCAGAAAA TAGAACTCCA 
CGGGATATTG CTAGTGTTCC ATCAATTGTT CCATTTCGTC GATTTCTGAT TCTTCATGAC 
AAAATGATAC TTGTTACGAA AAACAATCAC ATTGTTCAAA CTGACAAAAA ACTGAAAGTG 
GTGAATGTTG CGACTGAATT GGAAAGAGTT GATCATATTC TACCATTACG ATATGCAACA 
ATATCTCACA AAATTGAGTT CAGTGATGAA ATCAAATTTA TTGATGGAAG TAAAACGAAT 
CTTCAGTGGA CATTATCCCC ACCATTAGAA GCTGGAACTG TACTCTTCAA AGTTTCAATT 
TTTCGAGAAA AAATGGGAGG CCAAGACCCA CCGATAATCA CGATTCAGAG TGAAACTAAC 
TTCACAATCC CGTCCGAAGT TCTGGAAGCT TGGAGTTCGG CGCAGAGATT TGACGTCTCT 
GTGCAAGCAA TGACACCGTG GGCCACAGCA GTACTCAACA GAACCGGACT AACAGCGCCA 
GTAAAACCAC CAACTTCTCC AACTCAATTG AGAATATTTG CAACTCAACA AAAAACTGTC 
GACGGTCCTA GAGCTCTCAT TTCATTTTTC TGGGGTCCTC CGAGTGAATG GAATGGAACA 
CCTTATCAGT ACATTGTCAA CTGCACAAAA GATGATGGAA GTTGGATTGG TGGACCTGTT 
ACTACTTCTC AATCACATTA TTCATTTGCT GTAAAATCTG GAAAAGTGTC TTGTCAGGCA 
GCAGCTGCAA ATGAGCCAAC GAATATTGGA AGTTTTTCAG AGTTGATCAC AATTGACAGT 
TCTGAACTTA AACCTCTTGT TAAACTTTTT GCAATTGATT CCACAAATTC TCTGATATCA 
ATTAATGATC TCGCTCATGA AGAACCGAGA AGAGAGACAC GGCAAGTTGC ACAACCAGTC 
AAGTTAGAGT ACCAAGCAAT GGCATTCATT GGGGAAGACT TGTACGCTGT CAGGAAAGAA 
GGAGAGTCTG CACAGCCAGT GTTGGTTCAG ATTGATACAA ATCATATTGA TAATACAGTA 
CATAAGGTGT CAATTGGTGG AGATGTAACA CGAATTGATG CAATGACTTC TGATTGGGTC 
GGAAATCGTT TAATATTTGT TGCAGGCACA AATGTATATC AGTTATCTCT TGAGCCATTT 
CTTTCAACTT CACTTCTAAA CCCGCATAAG TTGATTCAAC TAACATCCGC GACTGATGCA 
AAACAGCTTG CTTATGATCC TTTTATGAAC ACTGCCTACT TGCTTACCAA AAATGGATCA 
CTATTTGCAC TTGATATGAA CAAGAATACT GAAGCTAATC TGGCACTAAC TGTATCATGT 
TTGGCAAGTC AAACTGTTAC TTGGATGATG ACTGAATTTG CCTGGAATCG AGCTTCTTCA 
CCAAAAATTT ATGCGCTTAC GTGGAATGGA TTGATATATG TGGATCTTAC TGAAGACTCA 
CAGTGCAACG AAGTAAGAAT TGATTGGACC AAATTTGGAG ATAAAGGTCT CAAAGATATA 
TCATCATTTG CAATTGCTGA TAAACTATTT GCATTTGTCA CTTCTTCGGA AATGCTTATT 
TATGGGAAAG ACACTGTGAC ACCGATAACT ATTGCAAATC CACCACTGAA ACAAATACTC 
GCGGTCAGTC AAAGTAGTCA ACCATATCCG GAAAGATCTT GCTTCGAACT TCCGTCTTCA 
AAAGGGATTG TATTTTCAAT TGTGAACGAA GGGAAAACTG GATCATTCTT GGAAGTTACA 
AAGAGCTCAT CATCATCTTC CTGTCAAGAA GTTAGTATGC CACAGACCCA ATACGAAATA 
TATTTTACAC GGAAAAATAC TGATAAGGTG AAGCACGTAA GAAGCTTCTC TGACAGAATC 
CACGTGGAAA ATGGAATTCT TGACAAGGAA ACCGACTATG ATGTAACTGT AACTTGGCTC 
AATAGATATT CACCAGCAAG TGGAGTTTCT GCTTCAAAAT CTTTCCGAAC AGGGTTTGGG 
TACCCATCCG CTCCAAGAGA TCCTCATGCG ATTCCAGTTA CTCCAGATAC GGTTTATCTG 
TATTGGAATT TACCAGAGAC GCTGAATGCT CCAATATCAG AGATAAAATA TAAGATTTCA 
CAGCAAGCAG CAGGAATTTC TGTACCGACT TCAATTGCAG TCATTGCTCT TTCTGAAACT 
GTTTCTTCAA ACATTTCATC AGATACAACA TCTTGCTTAA TCAATCCATG TCGTGTTAAA 
ATTGCAAATC TTCGACCTTC AAATGAGTAC AAGTTTTGGG TAACAGCTAC TCATATCTCT 
CACCTGGATG CTGCTACAAT CCTGAAAGAT GACGATGCAG TGTCATCAGA AGCTGTTGCT 
AGAACTCTTG ATGTACCAGG AACATTGAGA CCTGATAATG TTACTGGATC TTCGTTACTT 
CTACGATGGA ATGGATTGGA ACCAGAGCAT CGACCAAGCT CAATTGCAGT CCAGTACAGG 
GAGTCTGGTG GCGCTAATAA TGAATGGCAA TTTCCAATGA ATGTATCATT TGAACCAGAT 
GTAACAACTG AACTTGTACC AATTACCAAC CTTTTGTCAG CTACTTCATA TGACTATCGA 
TTTGTGGCAA CCTACACAGG AACCTATACA ATTGATGGAA AAGTACTAGC ATTCAAAGAA 
GACTACCTCC AATTGCCGCA ACAAGCTCGA ACAAAAGCTG GAGTACCTAC AGCTCCTCAA 
TTTGTGGAAG CAAAGCAAGA TGAAGAGGGA TGGATTGTAA CTTGGAAAGA ACCAATGAGT 
GATGGAGGAT CTCCAATTAC AAGTTATGCC GTTGAGACTA GAATTAATAA AACCGCAGAA 
TGGGAGATTG CTGAAAGAGG ATTAGATGGA TGGAAAACAT GGTGGAGACC CGGAAAATCA 
GATACAACAT CTTCAACTTC AACATCATCG ACCGAAGTTT CAGAGTTTCG AATTAGAGCA 
GCAAATATTG AAGGATTTGG AGCCTATGCT TATACAGAAG ATAAAAAAGA GGAGAAAGAA 
GAAGAACCAA GTTCAGTATT ACCGTATCTA CTGCTTCTTT CAATCATATT CCTTTTGGCA 
GCAATGATTT TAGTGGCTTG TTTTTGGTTG AAATCTCGCC GTCGTCAACA GCAGAAGAAA 
CGTGAAGCGG AAGACGAAAG AAATTGTATT CGACTTGACG TAGTTGCCAA TATGAATTTT 
TCCTCTTCAC ATCAATCTCT TCCTCCAGAA TATGAGAGTG AAATGAGAAA TCTTCCGGTA 
GTCAATTACA GTACTGTTAC TTTCAATGAT TACATTGATA CGTGTGCATA TGGAATTGTT 
CATAGTGGTA CTGCTGAAGA AGTACCAATG AGTTGGGAAA AAGACGTTAG AGTTGCTATT 
AAAAAGTTGA AGCCGAATCA TTCATTCCAA GAGAAAATGA TGTTTATGAA AGAAGCAATT 
CTTTTAAATA ATCTCGATCA CCCGAATATC GTCAAAGAAC TTGGTGTCTG CATTACACCT 
GGACAGGAAT TGATTTTATT GGAATACATG GAAGGTGGAA ACTTGTTGAA GTTTCTTCAA 
AAATCAACCC CAAATGAGTA CCAAAGTTCT GAATTATCTC CACGCGATCT TCTCTCAATT 
TCTGTGGATA TTGCCCGTGG CATGAACTAT CTTGAGAGAC TTCCTCATGT TCATAAGAAT 
CTTTCCGCGC GGAAGTGCCT TCTTGCCGGC CGGCCAGGTG TGACTAAACT TGAGATGGGA 
ATGTCGAAAG AGTTGAGCAA TGGACAAGTG AATCGTTCGG ATTTGGAAAA TATGGAAATT 
GTCCGTTGGA TGGCGCCGGA AGTTTTGAAA GATTTCCAGT TTTCATCAAA GAGTGATGTT 
TGGGCTTATG GTGTACTTCT CTACGAAGTT TTTTCATTCG GAGAAGTTCC ATATGGAGAT 
AAAGACAATA GAAGAATTAT GACTGATGTC AGAAATGGAA GTGTTCTACC AATACCTTCC 
TATTGTCCAT CGAAAAGGAT ATACAAAGTT ATAAAACAAT GTTTGACTTC TGATTCTACT 
AAAAGGGCAA ACTTTGCAAC AATTTTGAAG ATTTTCGAAA CATTTAGAGA CGATCAAAAG 
TGTCAGGATG ATAAACCAAT TCAATTCAAT GATGGAACAG ACAACACGAA TTTTAGTGCA 
TCACAAGATA GTACTTCAAG TCGTGAACCT CCATCTCCAT CCCATCGAAT GAGAGATTTC 
ATTGATACAA GAGATTTAGA ACCACCGTCC CCATCACATT TGAATCAAAG TTTTGGAGGA 
TTTGAACATC CATATGAAGG CGAACGCCCT GCAACAATGT GGAATGGAAG TGGCGCACGA 
AACTCTGCCA AAAATAGTAT TGGAAGATCA ATGAAAAAAG AGAAATTAAG AAATCCCATT 
CATTCGATGG ATGATCTAGT TGCTAGAAAT CAGAGACCAT TGAGCATTCA TTCAGAAGAT 
ACCGAATCTA CGGATTACGG AGCTTCATCA TCAATGTATT CTCCAGGATC TTCCAATCGA 
ATTTCAAGCC AAGTTGACCC TCCAATTGGT AGATTGAGCT CTGCTCCAGG AATTGGCATA 
GTCAATGATG CATTTGAGAG CAGTAATCCA TCGCTGAATT TGAGTAGAAG TTGGGCCGGG 
CTTTCAAGAG AAGTGAATCA GAATCCTGCT GGTGCTGGTA GTAGTGGAAC ACTTCCACAG 
CATACGAACT CTGCTGGACA TTTGAGACTC CCTGGGGTCC AAGTTGGAGC AGGTGGCGGC 
CGTGTCTACA GAAATGCCTC TGGCGGTGGT GGACCTTCAA ATCGAGGCCG CATCAGTCAA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rol-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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