let-418 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-418
Entrez Gene ID	178970
Full Name	Protein let-418
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_072122.4	NP_504523.1	Protein let-418
NM_072122.5	NP_504523.1	Protein let-418

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chd4	NP_666091.1
Caenorhabditis elegans (roundworm)	chd-3	NP_510140.1
Danio rerio (zebrafish)	chd4a	NP_001038323.1
Canis lupus familiaris (dog)	CHD4	XP_867754.2
Danio rerio (zebrafish)	chd4b	XP_685699.4
Arabidopsis thaliana (thale cress)	PKL	NP_565587.1
Arabidopsis thaliana (thale cress)	PKR2	NP_194918.2
Homo sapiens (human)	CHD4	NP_001264.2
Bos taurus (cattle)	CHD4	NP_001193430.1
Gallus gallus (chicken)	CHD4	XP_003640489.2
Caenorhabditis elegans (roundworm)	let-418	NP_504523.1
Xenopus tropicalis (tropical clawed frog)	chd4	NP_001096166.1
	CHD4	XP_001107252.2
Rattus norvegicus (Norway rat)	Chd4	XP_006237457.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These suppressors might represent direct competitors of LET-418 complexes for chromatin regulation of crucial genes involved in the transition to postembryonic development
Title: A Network of Chromatin Factors Is Regulating the Transition to Postembryonic Development in

germ cells deficient for spr-5 and let-418 mainly reprogram as neurons, suggesting that neuronal fate might be the first to be derepressed in early embryogenesis.
Title: LET-418/Mi2 and SPR-5/LSD1 cooperatively prevent somatic reprogramming of C. elegans germline stem cells.

LET-418/Mi2 could be part of a system that drives development and reproduction with concomitant life-reducing effects later in life.
Title: The Caenorhabditis elegans LET-418/Mi2 plays a conserved role in lifespan regulation.

the interaction between LIN-1 and LET-418/Mi-2 may link RTK/Ras signaling with chromatin remodeling and gene expression
Title: The Mi-2 nucleosome-remodeling protein LET-418 is targeted via LIN-1/ETS to the promoter of lin-39/Hox during vulval development in C. elegans.

The following let-418 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-418 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa346781	NM_072122.5 Latest version!	Caenorhabditis elegans Protein let-418 (let-418), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa346781	NM_072122.4	Caenorhabditis elegans Protein let-418 (let-418), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-418 ( NM_072122.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_504523.1 Caenorhabditis elegans Protein let-418 (let-418), partial mRNA.

Price & Availability↑

CloneID	OCa346781
Clone ID Related Accession (Same CDS sequence)	NM_072122.4 , NM_072122.5
Accession Version	NM_072122.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein let-418
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_072122.3. COMPLETENESS: incomplete on both ends.

ATGTCAACTG AAGAAGATCC GTCTTTAGTG GACGCGGAAG AATCAATGGA AGAGGGTTCT 
GTCACTCAAG ACGCAACTGA AGAAACAGAA GAAGAGGAAG AACAAGAGCA AGGCGATGAG 
GCTGGACCAT CTGAGCGAAA GCGTTCTTCT AGAAAGAAAG GCGGAAAGGG AGGCAAAAAA 
GGATCCAAAA AGAGTGCTGC CGCAGCTAGC AAGGTTGAGA TTCCTGATCC GTATAATTCG 
ACAAGTGAAG AAGTTTGTGC TGCTATCGGA TTGACAGACG TCGAATTTGA TTATGATGAA 
GAGGAGTTTC AAGGAATTTC TAATCTGAAG ACTTTCTCCT CAATCATCAA GCCACAAATA 
CTGGAGGCAA ACCCCGGAAC CAACGTATCC AAGATGTACC CGATGTTCCA AGTCAAATAT 
AAAGAGTATC AAGATCACAT GGCTGCCCAA GGAAAACCTG TTCAAAAACA AGCTAGAGGT 
TCAAAGACTC CAGCAGTTTC CACACCCGTC ATCCCTCCAC GATCAGCTCC AACGAAGACT 
CGTTCTGCAA GGAGAAAGCG TCGAGATTCC GATGCTCCTG ATAGTGATCA AGAGTTTGAG 
GCATTTATTA AGCAACAAGA ACAACTGGAG GACGATTTGG TAAAAGACAA GGAAGATGCT 
CGTATCAAAC GTGCAGCGGA AAGAGAAGAG AAGAAAAAAG GAGCATTGGA AGCTGCTCGT 
GCCGCTAAAA AAGCAAAATT AGAAAAGGGC GAAGAAGCTG AAAATAATGA CTACTGTGAG 
GAGTGCAAGC AAGATGGAGA ACTTCTTCTT TGTGACACCT GTCCCCGCGC TTACCATACA 
GTCTGTATTG ATGAAAATAT GGAAGAACCA CCAGAGGGTG ATTGGAGTTG TGCTCATTGT 
ATCGAACATG GCCCAGAAGT AGTTAAAGAA GAACCGGCGA AGCAAAACGA TGAATTCTGC 
AAGATCTGCA AAGAAACTGA GAATCTTCTG CTTTGTGATA GTTGTGTATG CTCATTCCAC 
GCATATTGTA TTGATCCTCC ACTTACCGAA GTTCCGAAAG AAGAAACATG GTCGTGCCCA 
CGGTGCGAGA CGGTTAAGCC GGAACACAAA ATCGAAAAAA TCCTTTGCTG GAGATGGAAG 
GAAATCCCGT ATCCAGAACC TCTCGAAGCT GGGAAAGAGG CTTCGAGTGA CGATGCGATG 
CTCAAACCAC CACGGAAAAT GGAACCAAGA CGTGAGCGTG AATTCTTCGT GAAATGGAAG 
TATTTATCAT ACTGGCAGTG TTCTTGGGTC TCCGAAATGC TGTTGGAAGT TCATTTCCGT 
ATGCTTATAT TGCTATATTG GCGCAAAAAT GATTCCGATG CTCCTCCAGA ATTTGAAGAA 
TCCGTTACGT CTCGTCATCA TTCTGATAAT GATCCTTATA AATTGCGCGA GCGGTTCTAC 
CAGTATGGTA TCAAACCGGA ATGGATGCAA ATTCATCGTA TTATCAATCA TCAATCATAT 
GCAAAGTCGC AGCAGGATTA CCTTGTTAAG TGGAAGGAGC TCAGCTATGA TCAAGCCACT 
TGGGAACGAG ATGATTCTAA TATTGCCAAT TACGAAGAAG CAATCATTAA ATACTGGCAA 
CATCGTGAGA GCAAATTAAA TGAGGATATT CCTAAAAATG TGCAGAAGAT GATTGCTAAA 
CATCGCGAAG CTAAAGGTCT TCCTCCAAAA GAAGACGAAA AGAAAAAGAA GAAGCGTGAG 
AAGATTGACA TCCGTAAAAA ATATGAAGTT CAGCCCGACT ACGTTACGGA AACCGGAGGA 
AAACTTCATC CATATCAACT GGAAGGACTT AACTGGCTTC GTCATTGTTG GTCCAACGGA 
ACTGATGCGA TTCTCGCTGA TGAAATGGGT CTTGGGAAGA CTGTTCAATC GCTTACTTTC 
TTATACAGTC TCATGAAGGA AGGCCATTGC AAAGGACCAT TTTTGATTGC CGCTCCTCTA 
TCAACTATTA TCAATTGGGA GCGTGAGGCT GAACAATGGT GTCCTGATTT CTATGTGGTC 
ACTTATGTTG GTCTTAGAGA TGCTCGTGTT GTGCTCCGAG AACATGAGTT TTCTTTCGTT 
GAAGGTGCCG TTCGATCCGG ACCGAAGGCT TCAAAAATGA AGACGACAGA GAATATGAAG 
TTCCACGTGT TGCTCACTTC TTATGAGACT ATCAATATGG ATAAAACTAT ACTTTCTTCC 
ATTGAATGGG GAGCTCTTGT TGTTGATGAG GCTCATCGGT TAAAGAATAA TCAGTCGTTG 
TTCTTCAAAA ATCTCAATGA ATATACTATT CATTATCGCG TACTTCTCAC CGGAACTCCA 
CTTCAAAACA ATTTAGAAGA GCTCTTCCAT CTCCTCAATT TCTTGTCCAA AGAACGTTTC 
AATCAGCTCG AGGCTTTCAC TGCCGAATTC AATGAGATTT CCAAAGAGGA TCAAATTGAA 
AAACTTCATA ACTTGCTCGG ACCTCACATG CTTCGCCGTC TCAAAGCTGA TGTGTTGACT 
GGCATGCCAT CGAAATCAGA ATTAATTGTT CGAGTTGAAC TGAGTGCTAT GCAGAAAAAA 
TGGTATAAAA ATATTCTGAC TCGCAATTTT GATGCATTGA ATGTGAAGAA TGGAGGAACT 
CAAATGTCAT TGATGAACGT CCTTATGGAA TTGAAGAAAT GTTGTAATCA TCCATATCTT 
TTTGTGAAGG CAGAGTTGGA GGCTCCGAAA GAGAAAAACG GAATGTATGA AGGAACGGCG 
CTCATCAAGA ATTCCGGAAA GTTTGTTCTT CTTCAAAAAA TGTTGAGAAA GCTGAAGGAT 
GGTGGCCACC GAGTGCTTAT CTTCTCACAA ATGACAAGGA TGCTGGACAT AATGGAAGAT 
TTATGTGAAT ATGAAGGATA CAGATATGAA AGAATAGATG GAAGTATCAT GGGACAAATG 
AGACAAGATG CTATCGATCG TTACAATGCT CCTGGAGCTC AACAATTTAT TTTCTTGCTC 
TCCACTCGAG CTGGAGGTCT TGGTATCAAT CTGGCAACTG CTGATACGGT TATCATATAT 
GATTCTGATT GGAATCCTCA CAATGATATT CAAGCTTTCT CTCGTGCACA TCGTCTCGGA 
CAGAAGCATA AAGTTATGAT TTATCGTTTT GTAACAAAGA AATCTGTCGA AGAAAAAATC 
ACTTCAGTTG CCAAGAAGAA GATGCTTTTG AATCACTTGG TTGTACGTGC AGGATTGGGA 
GGAAAAGAAG GAAAGACAAT GAGCAAGACT GAGCTGGACG ATGTGCTTCG ATGGGGAACC 
GAAGAGTTAT TCAGTGAGGA TCTTGATGCT GCCGAAGGCG AAGGCTCGGA GAAGAAAGGT 
GCTGCCGCAC AAGAAATTGT TTGGGATGAT GCAGCTGTAG ATGCTCTTCT CGATCGCAGT 
AACAAAGAAG AGACTCCAGC AGGAGAAGAT GGCGAAGAAA AAGCTGAATG GCAAAACGAA 
TATCTAAGCT CGTTCAAGGT GGCCAGCTAT CAGACAAAAG AGACTGAAGG ACAAGAAGAA 
GAGGAGGAAG AGGAAACGGA AGTGATTAAA GAAGATGAGA AAGAACCAGA TCCAGATTAT 
TGGGAGAAGC TGCTCAAGCA TCACTACGAA CAGGACAGGG AAATCGAATT GCAAAAGCTT 
GGAAAGGGAA AACGTGTTCG CAAACAAATC AACTATGCCA GTGAGAATAT GGGAACTGAT 
TGGAGCAAGC AGAACCAAAC CCAAGATGAT GACGATGATA ACGAGTCGTA CCGTGGTTCG 
GACAATGGCG ACGGCCTGAA CTCTGATGAA GATGATTACG ATGAGAAGAA AAAGCGGCGT 
CGTGATGAAG AAAAAATGCC TCCACTCATG GCTAAAGTCA ATGGACAAGT TGAGATTCTT 
GGTTTCAATC CACGTCAACG CAAGGCGTTC TACGGGGCTG TTATGCGCTG GGGAATGCCT 
CCACAAGATT CTCATCAATC TCAATGGCTT GTTCGAGATC TGAGAAACAA ATCGGAAAAA 
GTCTTCCGAG CTTATGCTTC ACTGTTCATG CGACATCTCT GTGAGCCTGG AGCTGATGGT 
CATGATACCT TTAATGATGG AGTTCCAAGA GAAGGTCTTA ATCGTCAGCA TGTTCTTGGC 
CGAATCGGAT TGCTGAGTTT AGTTCGGCGG AAGGTTCAAG AATTTGAGCA ATATAATGGC 
CAATGGTCTA TGCCTGAAAT ACAAGACGAA GTTCTCGCTA AAGCTGCCAA TGGTTCGGCA 
CAAGGATCTT CAAGAAGTAC TCCGAAACCA AAAGAAGAGC CGAAGGAAGA ACCAATGGAA 
AAGGAAGATG CTACTGAAAC AGTTAACGGA GCTACATCAG AGCCAGCTAC AGATGCTGAG 
AGTGAACAAA ACGCTCCTGT TGACGAACCA ATGGATACAG ATGAAGCCAA AGAACCGAAA 
GAAGAACCAA TTGAAACCGA GAAACCAAGA GCAGCCCGTC CATCATTCAA GTTCAACATT 
TGTGACGGTG GATTCACTGA ACTTCACTCT TTGTGGGCCA ATGAAGAGAA AGTTGCAAGA 
AACGGAAAAG AATATGAAAT CTGGTACCGC AGACACGACT ATTGGCTTTT AGCTGGTGTT 
GTTGTACACG GATATGGACG TTTCCAAGCT AATTTCAATG ACATCATCAA TGATCCCAGA 
TTCTCAGTTC TCAATGAGCC ATTCAAGGAA GTTGGCGCTG AGGCAACAGG ATCTGACATC 
AAAGCCAAGT TTATGCAACG TCGTTTCAAA CTTATTGAAC AGTCCCTTGT GATTGAGGAG 
CAACTTCGTC GCGCTGCACA CGCCAATCGT CACTTGCAGC CGGATAATGT TGGACCATTG 
GCTCAACGTT TTGCGGATTT AGAGAATATT GCTGAATCTC AAGCTAATAT CGCCAAAGAA 
AGTTCTGCTG GAAATCGTAA TGCGAATGCC GTTCTACACA AAACATTGGT TCAGCTCGAC 
GAAATACTTT CCGATATGAA GGCCGACGTG TCTAGACTTC CATCTACTTT TACACAGTTA 
GCGACTGTCA CCGAACGTCT CAACATGACC GAGAGACAAA TTCTTTCGCG ACTGACCACA 
AAAGACGAGG ACGCGATAGC CAACCGTAGC GTACTTCCAC CACCAGGACC ATTTGTAACT 
CCAATACTTC GCCAGCAGAT GGATGGCATT CAACCAAAGT TTGCAGCTCT TTACAGTAAA 
TTCATGTCTG AAAATGGAGA AAGGATGGAA GAGGATGAAC CAGTAGAGGC AGAAGAGGAA 
GAAGGTGTAA AACAAGAACC AGATGATGAG ACTCAAGATA GCGCCGAAGC TCCGCCAGTT 
CTTAGTGCAG AAGTGAATTC CGACGATAGC AACGATGTTC CATCTACTTC CGCTGCTGCT 
GCTGTCAGTT CTGAAACAGC TGCCGACGCC GAACCAGCTT CCGCCGAAGA TCAAGCTCCA 
ACAGATGAGC CAGAACCAAT GGAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_504523.1
CDS	1..5490
Translation	MSTEEDPSLVDAEESMEEGSVTQDATEETEEEEEQEQGDEAGPSERKRSSRKKGGKGGKKGSKKSAAAASKVEIPDPYNSTSEEVCAAIGLTDVEFDYDEEEFQGISNLKTFSSIIKPQILEANPGTNVSKMYPMFQVKYKEYQDHMAAQGKPVQKQARGSKTPAVSTPVIPPRSAPTKTRSARRKRRDSDAPDSDQEFEAFIKQQEQLEDDLVKDKEDARIKRAAEREEKKKGALEAARAAKKAKLEKGEEAENNDYCEECKQDGELLLCDTCPRAYHTVCIDENMEEPPEGDWSCAHCIEHGPEVVKEEPAKQNDEFCKICKETENLLLCDSCVCSFHAYCIDPPLTEVPKEETWSCPRCETVKPEHKIEKILCWRWKEIPYPEPLEAGKEASSDDAMLKPPRKMEPRREREFFVKWKYLSYWQCSWVSEMLLEVHFRMLILLYWRKNDSDAPPEFEESVTSRHHSDNDPYKLRERFYQYGIKPEWMQIHRIINHQSYAKSQQDYLVKWKELSYDQATWERDDSNIANYEEAIIKYWQHRESKLNEDIPKNVQKMIAKHREAKGLPPKEDEKKKKKREKIDIRKKYEVQPDYVTETGGKLHPYQLEGLNWLRHCWSNGTDAILADEMGLGKTVQSLTFLYSLMKEGHCKGPFLIAAPLSTIINWEREAEQWCPDFYVVTYVGLRDARVVLREHEFSFVEGAVRSGPKASKMKTTENMKFHVLLTSYETINMDKTILSSIEWGALVVDEAHRLKNNQSLFFKNLNEYTIHYRVLLTGTPLQNNLEELFHLLNFLSKERFNQLEAFTAEFNEISKEDQIEKLHNLLGPHMLRRLKADVLTGMPSKSELIVRVELSAMQKKWYKNILTRNFDALNVKNGGTQMSLMNVLMELKKCCNHPYLFVKAELEAPKEKNGMYEGTALIKNSGKFVLLQKMLRKLKDGGHRVLIFSQMTRMLDIMEDLCEYEGYRYERIDGSIMGQMRQDAIDRYNAPGAQQFIFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRLGQKHKVMIYRFVTKKSVEEKITSVAKKKMLLNHLVVRAGLGGKEGKTMSKTELDDVLRWGTEELFSEDLDAAEGEGSEKKGAAAQEIVWDDAAVDALLDRSNKEETPAGEDGEEKAEWQNEYLSSFKVASYQTKETEGQEEEEEEETEVIKEDEKEPDPDYWEKLLKHHYEQDREIELQKLGKGKRVRKQINYASENMGTDWSKQNQTQDDDDDNESYRGSDNGDGLNSDEDDYDEKKKRRRDEEKMPPLMAKVNGQVEILGFNPRQRKAFYGAVMRWGMPPQDSHQSQWLVRDLRNKSEKVFRAYASLFMRHLCEPGADGHDTFNDGVPREGLNRQHVLGRIGLLSLVRRKVQEFEQYNGQWSMPEIQDEVLAKAANGSAQGSSRSTPKPKEEPKEEPMEKEDATETVNGATSEPATDAESEQNAPVDEPMDTDEAKEPKEEPIETEKPRAARPSFKFNICDGGFTELHSLWANEEKVARNGKEYEIWYRRHDYWLLAGVVVHGYGRFQANFNDIINDPRFSVLNEPFKEVGAEATGSDIKAKFMQRRFKLIEQSLVIEEQLRRAAHANRHLQPDNVGPLAQRFADLENIAESQANIAKESSAGNRNANAVLHKTLVQLDEILSDMKADVSRLPSTFTQLATVTERLNMTERQILSRLTTKDEDAIANRSVLPPPGPFVTPILRQQMDGIQPKFAALYSKFMSENGERMEEDEPVEAEEEEGVKQEPDDETQDSAEAPPVLSAEVNSDDSNDVPSTSAAAAVSSETAADAEPASAEDQAPTDEPEPMET

Target ORF information:

RefSeq Version	NM_072122.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein let-418 (let-418), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_072122.4

ORF Insert Sequence:

ATGTCAACTG AAGAAGATCC GTCTTTAGTG GACGCGGAAG AATCAATGGA AGAGGGTTCT 
GTCACTCAAG ACGCAACTGA AGAAACAGAA GAAGAGGAAG AACAAGAGCA AGGCGATGAG 
GCTGGACCAT CTGAGCGAAA GCGTTCTTCT AGAAAGAAAG GCGGAAAGGG AGGCAAAAAA 
GGATCCAAAA AGAGTGCTGC CGCAGCTAGC AAGGTTGAGA TTCCTGATCC GTATAATTCG 
ACAAGTGAAG AAGTTTGTGC TGCTATCGGA TTGACAGACG TCGAATTTGA TTATGATGAA 
GAGGAGTTTC AAGGAATTTC TAATCTGAAG ACTTTCTCCT CAATCATCAA GCCACAAATA 
CTGGAGGCAA ACCCCGGAAC CAACGTATCC AAGATGTACC CGATGTTCCA AGTCAAATAT 
AAAGAGTATC AAGATCACAT GGCTGCCCAA GGAAAACCTG TTCAAAAACA AGCTAGAGGT 
TCAAAGACTC CAGCAGTTTC CACACCCGTC ATCCCTCCAC GATCAGCTCC AACGAAGACT 
CGTTCTGCAA GGAGAAAGCG TCGAGATTCC GATGCTCCTG ATAGTGATCA AGAGTTTGAG 
GCATTTATTA AGCAACAAGA ACAACTGGAG GACGATTTGG TAAAAGACAA GGAAGATGCT 
CGTATCAAAC GTGCAGCGGA AAGAGAAGAG AAGAAAAAAG GAGCATTGGA AGCTGCTCGT 
GCCGCTAAAA AAGCAAAATT AGAAAAGGGC GAAGAAGCTG AAAATAATGA CTACTGTGAG 
GAGTGCAAGC AAGATGGAGA ACTTCTTCTT TGTGACACCT GTCCCCGCGC TTACCATACA 
GTCTGTATTG ATGAAAATAT GGAAGAACCA CCAGAGGGTG ATTGGAGTTG TGCTCATTGT 
ATCGAACATG GCCCAGAAGT AGTTAAAGAA GAACCGGCGA AGCAAAACGA TGAATTCTGC 
AAGATCTGCA AAGAAACTGA GAATCTTCTG CTTTGTGATA GTTGTGTATG CTCATTCCAC 
GCATATTGTA TTGATCCTCC ACTTACCGAA GTTCCGAAAG AAGAAACATG GTCGTGCCCA 
CGGTGCGAGA CGGTTAAGCC GGAACACAAA ATCGAAAAAA TCCTTTGCTG GAGATGGAAG 
GAAATCCCGT ATCCAGAACC TCTCGAAGCT GGGAAAGAGG CTTCGAGTGA CGATGCGATG 
CTCAAACCAC CACGGAAAAT GGAACCAAGA CGTGAGCGTG AATTCTTCGT GAAATGGAAG 
TATTTATCAT ACTGGCAGTG TTCTTGGGTC TCCGAAATGC TGTTGGAAGT TCATTTCCGT 
ATGCTTATAT TGCTATATTG GCGCAAAAAT GATTCCGATG CTCCTCCAGA ATTTGAAGAA 
TCCGTTACGT CTCGTCATCA TTCTGATAAT GATCCTTATA AATTGCGCGA GCGGTTCTAC 
CAGTATGGTA TCAAACCGGA ATGGATGCAA ATTCATCGTA TTATCAATCA TCAATCATAT 
GCAAAGTCGC AGCAGGATTA CCTTGTTAAG TGGAAGGAGC TCAGCTATGA TCAAGCCACT 
TGGGAACGAG ATGATTCTAA TATTGCCAAT TACGAAGAAG CAATCATTAA ATACTGGCAA 
CATCGTGAGA GCAAATTAAA TGAGGATATT CCTAAAAATG TGCAGAAGAT GATTGCTAAA 
CATCGCGAAG CTAAAGGTCT TCCTCCAAAA GAAGACGAAA AGAAAAAGAA GAAGCGTGAG 
AAGATTGACA TCCGTAAAAA ATATGAAGTT CAGCCCGACT ACGTTACGGA AACCGGAGGA 
AAACTTCATC CATATCAACT GGAAGGACTT AACTGGCTTC GTCATTGTTG GTCCAACGGA 
ACTGATGCGA TTCTCGCTGA TGAAATGGGT CTTGGGAAGA CTGTTCAATC GCTTACTTTC 
TTATACAGTC TCATGAAGGA AGGCCATTGC AAAGGACCAT TTTTGATTGC CGCTCCTCTA 
TCAACTATTA TCAATTGGGA GCGTGAGGCT GAACAATGGT GTCCTGATTT CTATGTGGTC 
ACTTATGTTG GTCTTAGAGA TGCTCGTGTT GTGCTCCGAG AACATGAGTT TTCTTTCGTT 
GAAGGTGCCG TTCGATCCGG ACCGAAGGCT TCAAAAATGA AGACGACAGA GAATATGAAG 
TTCCACGTGT TGCTCACTTC TTATGAGACT ATCAATATGG ATAAAACTAT ACTTTCTTCC 
ATTGAATGGG GAGCTCTTGT TGTTGATGAG GCTCATCGGT TAAAGAATAA TCAGTCGTTG 
TTCTTCAAAA ATCTCAATGA ATATACTATT CATTATCGCG TACTTCTCAC CGGAACTCCA 
CTTCAAAACA ATTTAGAAGA GCTCTTCCAT CTCCTCAATT TCTTGTCCAA AGAACGTTTC 
AATCAGCTCG AGGCTTTCAC TGCCGAATTC AATGAGATTT CCAAAGAGGA TCAAATTGAA 
AAACTTCATA ACTTGCTCGG ACCTCACATG CTTCGCCGTC TCAAAGCTGA TGTGTTGACT 
GGCATGCCAT CGAAATCAGA ATTAATTGTT CGAGTTGAAC TGAGTGCTAT GCAGAAAAAA 
TGGTATAAAA ATATTCTGAC TCGCAATTTT GATGCATTGA ATGTGAAGAA TGGAGGAACT 
CAAATGTCAT TGATGAACGT CCTTATGGAA TTGAAGAAAT GTTGTAATCA TCCATATCTT 
TTTGTGAAGG CAGAGTTGGA GGCTCCGAAA GAGAAAAACG GAATGTATGA AGGAACGGCG 
CTCATCAAGA ATTCCGGAAA GTTTGTTCTT CTTCAAAAAA TGTTGAGAAA GCTGAAGGAT 
GGTGGCCACC GAGTGCTTAT CTTCTCACAA ATGACAAGGA TGCTGGACAT AATGGAAGAT 
TTATGTGAAT ATGAAGGATA CAGATATGAA AGAATAGATG GAAGTATCAT GGGACAAATG 
AGACAAGATG CTATCGATCG TTACAATGCT CCTGGAGCTC AACAATTTAT TTTCTTGCTC 
TCCACTCGAG CTGGAGGTCT TGGTATCAAT CTGGCAACTG CTGATACGGT TATCATATAT 
GATTCTGATT GGAATCCTCA CAATGATATT CAAGCTTTCT CTCGTGCACA TCGTCTCGGA 
CAGAAGCATA AAGTTATGAT TTATCGTTTT GTAACAAAGA AATCTGTCGA AGAAAAAATC 
ACTTCAGTTG CCAAGAAGAA GATGCTTTTG AATCACTTGG TTGTACGTGC AGGATTGGGA 
GGAAAAGAAG GAAAGACAAT GAGCAAGACT GAGCTGGACG ATGTGCTTCG ATGGGGAACC 
GAAGAGTTAT TCAGTGAGGA TCTTGATGCT GCCGAAGGCG AAGGCTCGGA GAAGAAAGGT 
GCTGCCGCAC AAGAAATTGT TTGGGATGAT GCAGCTGTAG ATGCTCTTCT CGATCGCAGT 
AACAAAGAAG AGACTCCAGC AGGAGAAGAT GGCGAAGAAA AAGCTGAATG GCAAAACGAA 
TATCTAAGCT CGTTCAAGGT GGCCAGCTAT CAGACAAAAG AGACTGAAGG ACAAGAAGAA 
GAGGAGGAAG AGGAAACGGA AGTGATTAAA GAAGATGAGA AAGAACCAGA TCCAGATTAT 
TGGGAGAAGC TGCTCAAGCA TCACTACGAA CAGGACAGGG AAATCGAATT GCAAAAGCTT 
GGAAAGGGAA AACGTGTTCG CAAACAAATC AACTATGCCA GTGAGAATAT GGGAACTGAT 
TGGAGCAAGC AGAACCAAAC CCAAGATGAT GACGATGATA ACGAGTCGTA CCGTGGTTCG 
GACAATGGCG ACGGCCTGAA CTCTGATGAA GATGATTACG ATGAGAAGAA AAAGCGGCGT 
CGTGATGAAG AAAAAATGCC TCCACTCATG GCTAAAGTCA ATGGACAAGT TGAGATTCTT 
GGTTTCAATC CACGTCAACG CAAGGCGTTC TACGGGGCTG TTATGCGCTG GGGAATGCCT 
CCACAAGATT CTCATCAATC TCAATGGCTT GTTCGAGATC TGAGAAACAA ATCGGAAAAA 
GTCTTCCGAG CTTATGCTTC ACTGTTCATG CGACATCTCT GTGAGCCTGG AGCTGATGGT 
CATGATACCT TTAATGATGG AGTTCCAAGA GAAGGTCTTA ATCGTCAGCA TGTTCTTGGC 
CGAATCGGAT TGCTGAGTTT AGTTCGGCGG AAGGTTCAAG AATTTGAGCA ATATAATGGC 
CAATGGTCTA TGCCTGAAAT ACAAGACGAA GTTCTCGCTA AAGCTGCCAA TGGTTCGGCA 
CAAGGATCTT CAAGAAGTAC TCCGAAACCA AAAGAAGAGC CGAAGGAAGA ACCAATGGAA 
AAGGAAGATG CTACTGAAAC AGTTAACGGA GCTACATCAG AGCCAGCTAC AGATGCTGAG 
AGTGAACAAA ACGCTCCTGT TGACGAACCA ATGGATACAG ATGAAGCCAA AGAACCGAAA 
GAAGAACCAA TTGAAACCGA GAAACCAAGA GCAGCCCGTC CATCATTCAA GTTCAACATT 
TGTGACGGTG GATTCACTGA ACTTCACTCT TTGTGGGCCA ATGAAGAGAA AGTTGCAAGA 
AACGGAAAAG AATATGAAAT CTGGTACCGC AGACACGACT ATTGGCTTTT AGCTGGTGTT 
GTTGTACACG GATATGGACG TTTCCAAGCT AATTTCAATG ACATCATCAA TGATCCCAGA 
TTCTCAGTTC TCAATGAGCC ATTCAAGGAA GTTGGCGCTG AGGCAACAGG ATCTGACATC 
AAAGCCAAGT TTATGCAACG TCGTTTCAAA CTTATTGAAC AGTCCCTTGT GATTGAGGAG 
CAACTTCGTC GCGCTGCACA CGCCAATCGT CACTTGCAGC CGGATAATGT TGGACCATTG 
GCTCAACGTT TTGCGGATTT AGAGAATATT GCTGAATCTC AAGCTAATAT CGCCAAAGAA 
AGTTCTGCTG GAAATCGTAA TGCGAATGCC GTTCTACACA AAACATTGGT TCAGCTCGAC 
GAAATACTTT CCGATATGAA GGCCGACGTG TCTAGACTTC CATCTACTTT TACACAGTTA 
GCGACTGTCA CCGAACGTCT CAACATGACC GAGAGACAAA TTCTTTCGCG ACTGACCACA 
AAAGACGAGG ACGCGATAGC CAACCGTAGC GTACTTCCAC CACCAGGACC ATTTGTAACT 
CCAATACTTC GCCAGCAGAT GGATGGCATT CAACCAAAGT TTGCAGCTCT TTACAGTAAA 
TTCATGTCTG AAAATGGAGA AAGGATGGAA GAGGATGAAC CAGTAGAGGC AGAAGAGGAA 
GAAGGTGTAA AACAAGAACC AGATGATGAG ACTCAAGATA GCGCCGAAGC TCCGCCAGTT 
CTTAGTGCAG AAGTGAATTC CGACGATAGC AACGATGTTC CATCTACTTC CGCTGCTGCT 
GCTGTCAGTT CTGAAACAGC TGCCGACGCC GAACCAGCTT CCGCCGAAGA TCAAGCTCCA 
ACAGATGAGC CAGAACCAAT GGAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-418 ( NM_072122.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_504523.1 Caenorhabditis elegans Protein let-418 (let-418), mRNA.

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CloneID	OCa346781
Clone ID Related Accession (Same CDS sequence)	NM_072122.4 , NM_072122.5
Accession Version	NM_072122.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein let-418
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_072122.4.

ATGTCAACTG AAGAAGATCC GTCTTTAGTG GACGCGGAAG AATCAATGGA AGAGGGTTCT 
GTCACTCAAG ACGCAACTGA AGAAACAGAA GAAGAGGAAG AACAAGAGCA AGGCGATGAG 
GCTGGACCAT CTGAGCGAAA GCGTTCTTCT AGAAAGAAAG GCGGAAAGGG AGGCAAAAAA 
GGATCCAAAA AGAGTGCTGC CGCAGCTAGC AAGGTTGAGA TTCCTGATCC GTATAATTCG 
ACAAGTGAAG AAGTTTGTGC TGCTATCGGA TTGACAGACG TCGAATTTGA TTATGATGAA 
GAGGAGTTTC AAGGAATTTC TAATCTGAAG ACTTTCTCCT CAATCATCAA GCCACAAATA 
CTGGAGGCAA ACCCCGGAAC CAACGTATCC AAGATGTACC CGATGTTCCA AGTCAAATAT 
AAAGAGTATC AAGATCACAT GGCTGCCCAA GGAAAACCTG TTCAAAAACA AGCTAGAGGT 
TCAAAGACTC CAGCAGTTTC CACACCCGTC ATCCCTCCAC GATCAGCTCC AACGAAGACT 
CGTTCTGCAA GGAGAAAGCG TCGAGATTCC GATGCTCCTG ATAGTGATCA AGAGTTTGAG 
GCATTTATTA AGCAACAAGA ACAACTGGAG GACGATTTGG TAAAAGACAA GGAAGATGCT 
CGTATCAAAC GTGCAGCGGA AAGAGAAGAG AAGAAAAAAG GAGCATTGGA AGCTGCTCGT 
GCCGCTAAAA AAGCAAAATT AGAAAAGGGC GAAGAAGCTG AAAATAATGA CTACTGTGAG 
GAGTGCAAGC AAGATGGAGA ACTTCTTCTT TGTGACACCT GTCCCCGCGC TTACCATACA 
GTCTGTATTG ATGAAAATAT GGAAGAACCA CCAGAGGGTG ATTGGAGTTG TGCTCATTGT 
ATCGAACATG GCCCAGAAGT AGTTAAAGAA GAACCGGCGA AGCAAAACGA TGAATTCTGC 
AAGATCTGCA AAGAAACTGA GAATCTTCTG CTTTGTGATA GTTGTGTATG CTCATTCCAC 
GCATATTGTA TTGATCCTCC ACTTACCGAA GTTCCGAAAG AAGAAACATG GTCGTGCCCA 
CGGTGCGAGA CGGTTAAGCC GGAACACAAA ATCGAAAAAA TCCTTTGCTG GAGATGGAAG 
GAAATCCCGT ATCCAGAACC TCTCGAAGCT GGGAAAGAGG CTTCGAGTGA CGATGCGATG 
CTCAAACCAC CACGGAAAAT GGAACCAAGA CGTGAGCGTG AATTCTTCGT GAAATGGAAG 
TATTTATCAT ACTGGCAGTG TTCTTGGGTC TCCGAAATGC TGTTGGAAGT TCATTTCCGT 
ATGCTTATAT TGCTATATTG GCGCAAAAAT GATTCCGATG CTCCTCCAGA ATTTGAAGAA 
TCCGTTACGT CTCGTCATCA TTCTGATAAT GATCCTTATA AATTGCGCGA GCGGTTCTAC 
CAGTATGGTA TCAAACCGGA ATGGATGCAA ATTCATCGTA TTATCAATCA TCAATCATAT 
GCAAAGTCGC AGCAGGATTA CCTTGTTAAG TGGAAGGAGC TCAGCTATGA TCAAGCCACT 
TGGGAACGAG ATGATTCTAA TATTGCCAAT TACGAAGAAG CAATCATTAA ATACTGGCAA 
CATCGTGAGA GCAAATTAAA TGAGGATATT CCTAAAAATG TGCAGAAGAT GATTGCTAAA 
CATCGCGAAG CTAAAGGTCT TCCTCCAAAA GAAGACGAAA AGAAAAAGAA GAAGCGTGAG 
AAGATTGACA TCCGTAAAAA ATATGAAGTT CAGCCCGACT ACGTTACGGA AACCGGAGGA 
AAACTTCATC CATATCAACT GGAAGGACTT AACTGGCTTC GTCATTGTTG GTCCAACGGA 
ACTGATGCGA TTCTCGCTGA TGAAATGGGT CTTGGGAAGA CTGTTCAATC GCTTACTTTC 
TTATACAGTC TCATGAAGGA AGGCCATTGC AAAGGACCAT TTTTGATTGC CGCTCCTCTA 
TCAACTATTA TCAATTGGGA GCGTGAGGCT GAACAATGGT GTCCTGATTT CTATGTGGTC 
ACTTATGTTG GTCTTAGAGA TGCTCGTGTT GTGCTCCGAG AACATGAGTT TTCTTTCGTT 
GAAGGTGCCG TTCGATCCGG ACCGAAGGCT TCAAAAATGA AGACGACAGA GAATATGAAG 
TTCCACGTGT TGCTCACTTC TTATGAGACT ATCAATATGG ATAAAACTAT ACTTTCTTCC 
ATTGAATGGG GAGCTCTTGT TGTTGATGAG GCTCATCGGT TAAAGAATAA TCAGTCGTTG 
TTCTTCAAAA ATCTCAATGA ATATACTATT CATTATCGCG TACTTCTCAC CGGAACTCCA 
CTTCAAAACA ATTTAGAAGA GCTCTTCCAT CTCCTCAATT TCTTGTCCAA AGAACGTTTC 
AATCAGCTCG AGGCTTTCAC TGCCGAATTC AATGAGATTT CCAAAGAGGA TCAAATTGAA 
AAACTTCATA ACTTGCTCGG ACCTCACATG CTTCGCCGTC TCAAAGCTGA TGTGTTGACT 
GGCATGCCAT CGAAATCAGA ATTAATTGTT CGAGTTGAAC TGAGTGCTAT GCAGAAAAAA 
TGGTATAAAA ATATTCTGAC TCGCAATTTT GATGCATTGA ATGTGAAGAA TGGAGGAACT 
CAAATGTCAT TGATGAACGT CCTTATGGAA TTGAAGAAAT GTTGTAATCA TCCATATCTT 
TTTGTGAAGG CAGAGTTGGA GGCTCCGAAA GAGAAAAACG GAATGTATGA AGGAACGGCG 
CTCATCAAGA ATTCCGGAAA GTTTGTTCTT CTTCAAAAAA TGTTGAGAAA GCTGAAGGAT 
GGTGGCCACC GAGTGCTTAT CTTCTCACAA ATGACAAGGA TGCTGGACAT AATGGAAGAT 
TTATGTGAAT ATGAAGGATA CAGATATGAA AGAATAGATG GAAGTATCAT GGGACAAATG 
AGACAAGATG CTATCGATCG TTACAATGCT CCTGGAGCTC AACAATTTAT TTTCTTGCTC 
TCCACTCGAG CTGGAGGTCT TGGTATCAAT CTGGCAACTG CTGATACGGT TATCATATAT 
GATTCTGATT GGAATCCTCA CAATGATATT CAAGCTTTCT CTCGTGCACA TCGTCTCGGA 
CAGAAGCATA AAGTTATGAT TTATCGTTTT GTAACAAAGA AATCTGTCGA AGAAAAAATC 
ACTTCAGTTG CCAAGAAGAA GATGCTTTTG AATCACTTGG TTGTACGTGC AGGATTGGGA 
GGAAAAGAAG GAAAGACAAT GAGCAAGACT GAGCTGGACG ATGTGCTTCG ATGGGGAACC 
GAAGAGTTAT TCAGTGAGGA TCTTGATGCT GCCGAAGGCG AAGGCTCGGA GAAGAAAGGT 
GCTGCCGCAC AAGAAATTGT TTGGGATGAT GCAGCTGTAG ATGCTCTTCT CGATCGCAGT 
AACAAAGAAG AGACTCCAGC AGGAGAAGAT GGCGAAGAAA AAGCTGAATG GCAAAACGAA 
TATCTAAGCT CGTTCAAGGT GGCCAGCTAT CAGACAAAAG AGACTGAAGG ACAAGAAGAA 
GAGGAGGAAG AGGAAACGGA AGTGATTAAA GAAGATGAGA AAGAACCAGA TCCAGATTAT 
TGGGAGAAGC TGCTCAAGCA TCACTACGAA CAGGACAGGG AAATCGAATT GCAAAAGCTT 
GGAAAGGGAA AACGTGTTCG CAAACAAATC AACTATGCCA GTGAGAATAT GGGAACTGAT 
TGGAGCAAGC AGAACCAAAC CCAAGATGAT GACGATGATA ACGAGTCGTA CCGTGGTTCG 
GACAATGGCG ACGGCCTGAA CTCTGATGAA GATGATTACG ATGAGAAGAA AAAGCGGCGT 
CGTGATGAAG AAAAAATGCC TCCACTCATG GCTAAAGTCA ATGGACAAGT TGAGATTCTT 
GGTTTCAATC CACGTCAACG CAAGGCGTTC TACGGGGCTG TTATGCGCTG GGGAATGCCT 
CCACAAGATT CTCATCAATC TCAATGGCTT GTTCGAGATC TGAGAAACAA ATCGGAAAAA 
GTCTTCCGAG CTTATGCTTC ACTGTTCATG CGACATCTCT GTGAGCCTGG AGCTGATGGT 
CATGATACCT TTAATGATGG AGTTCCAAGA GAAGGTCTTA ATCGTCAGCA TGTTCTTGGC 
CGAATCGGAT TGCTGAGTTT AGTTCGGCGG AAGGTTCAAG AATTTGAGCA ATATAATGGC 
CAATGGTCTA TGCCTGAAAT ACAAGACGAA GTTCTCGCTA AAGCTGCCAA TGGTTCGGCA 
CAAGGATCTT CAAGAAGTAC TCCGAAACCA AAAGAAGAGC CGAAGGAAGA ACCAATGGAA 
AAGGAAGATG CTACTGAAAC AGTTAACGGA GCTACATCAG AGCCAGCTAC AGATGCTGAG 
AGTGAACAAA ACGCTCCTGT TGACGAACCA ATGGATACAG ATGAAGCCAA AGAACCGAAA 
GAAGAACCAA TTGAAACCGA GAAACCAAGA GCAGCCCGTC CATCATTCAA GTTCAACATT 
TGTGACGGTG GATTCACTGA ACTTCACTCT TTGTGGGCCA ATGAAGAGAA AGTTGCAAGA 
AACGGAAAAG AATATGAAAT CTGGTACCGC AGACACGACT ATTGGCTTTT AGCTGGTGTT 
GTTGTACACG GATATGGACG TTTCCAAGCT AATTTCAATG ACATCATCAA TGATCCCAGA 
TTCTCAGTTC TCAATGAGCC ATTCAAGGAA GTTGGCGCTG AGGCAACAGG ATCTGACATC 
AAAGCCAAGT TTATGCAACG TCGTTTCAAA CTTATTGAAC AGTCCCTTGT GATTGAGGAG 
CAACTTCGTC GCGCTGCACA CGCCAATCGT CACTTGCAGC CGGATAATGT TGGACCATTG 
GCTCAACGTT TTGCGGATTT AGAGAATATT GCTGAATCTC AAGCTAATAT CGCCAAAGAA 
AGTTCTGCTG GAAATCGTAA TGCGAATGCC GTTCTACACA AAACATTGGT TCAGCTCGAC 
GAAATACTTT CCGATATGAA GGCCGACGTG TCTAGACTTC CATCTACTTT TACACAGTTA 
GCGACTGTCA CCGAACGTCT CAACATGACC GAGAGACAAA TTCTTTCGCG ACTGACCACA 
AAAGACGAGG ACGCGATAGC CAACCGTAGC GTACTTCCAC CACCAGGACC ATTTGTAACT 
CCAATACTTC GCCAGCAGAT GGATGGCATT CAACCAAAGT TTGCAGCTCT TTACAGTAAA 
TTCATGTCTG AAAATGGAGA AAGGATGGAA GAGGATGAAC CAGTAGAGGC AGAAGAGGAA 
GAAGGTGTAA AACAAGAACC AGATGATGAG ACTCAAGATA GCGCCGAAGC TCCGCCAGTT 
CTTAGTGCAG AAGTGAATTC CGACGATAGC AACGATGTTC CATCTACTTC CGCTGCTGCT 
GCTGTCAGTT CTGAAACAGC TGCCGACGCC GAACCAGCTT CCGCCGAAGA TCAAGCTCCA 
ACAGATGAGC CAGAACCAAT GGAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_504523.1
CDS	29..5518
Translation	MSTEEDPSLVDAEESMEEGSVTQDATEETEEEEEQEQGDEAGPSERKRSSRKKGGKGGKKGSKKSAAAASKVEIPDPYNSTSEEVCAAIGLTDVEFDYDEEEFQGISNLKTFSSIIKPQILEANPGTNVSKMYPMFQVKYKEYQDHMAAQGKPVQKQARGSKTPAVSTPVIPPRSAPTKTRSARRKRRDSDAPDSDQEFEAFIKQQEQLEDDLVKDKEDARIKRAAEREEKKKGALEAARAAKKAKLEKGEEAENNDYCEECKQDGELLLCDTCPRAYHTVCIDENMEEPPEGDWSCAHCIEHGPEVVKEEPAKQNDEFCKICKETENLLLCDSCVCSFHAYCIDPPLTEVPKEETWSCPRCETVKPEHKIEKILCWRWKEIPYPEPLEAGKEASSDDAMLKPPRKMEPRREREFFVKWKYLSYWQCSWVSEMLLEVHFRMLILLYWRKNDSDAPPEFEESVTSRHHSDNDPYKLRERFYQYGIKPEWMQIHRIINHQSYAKSQQDYLVKWKELSYDQATWERDDSNIANYEEAIIKYWQHRESKLNEDIPKNVQKMIAKHREAKGLPPKEDEKKKKKREKIDIRKKYEVQPDYVTETGGKLHPYQLEGLNWLRHCWSNGTDAILADEMGLGKTVQSLTFLYSLMKEGHCKGPFLIAAPLSTIINWEREAEQWCPDFYVVTYVGLRDARVVLREHEFSFVEGAVRSGPKASKMKTTENMKFHVLLTSYETINMDKTILSSIEWGALVVDEAHRLKNNQSLFFKNLNEYTIHYRVLLTGTPLQNNLEELFHLLNFLSKERFNQLEAFTAEFNEISKEDQIEKLHNLLGPHMLRRLKADVLTGMPSKSELIVRVELSAMQKKWYKNILTRNFDALNVKNGGTQMSLMNVLMELKKCCNHPYLFVKAELEAPKEKNGMYEGTALIKNSGKFVLLQKMLRKLKDGGHRVLIFSQMTRMLDIMEDLCEYEGYRYERIDGSIMGQMRQDAIDRYNAPGAQQFIFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRLGQKHKVMIYRFVTKKSVEEKITSVAKKKMLLNHLVVRAGLGGKEGKTMSKTELDDVLRWGTEELFSEDLDAAEGEGSEKKGAAAQEIVWDDAAVDALLDRSNKEETPAGEDGEEKAEWQNEYLSSFKVASYQTKETEGQEEEEEEETEVIKEDEKEPDPDYWEKLLKHHYEQDREIELQKLGKGKRVRKQINYASENMGTDWSKQNQTQDDDDDNESYRGSDNGDGLNSDEDDYDEKKKRRRDEEKMPPLMAKVNGQVEILGFNPRQRKAFYGAVMRWGMPPQDSHQSQWLVRDLRNKSEKVFRAYASLFMRHLCEPGADGHDTFNDGVPREGLNRQHVLGRIGLLSLVRRKVQEFEQYNGQWSMPEIQDEVLAKAANGSAQGSSRSTPKPKEEPKEEPMEKEDATETVNGATSEPATDAESEQNAPVDEPMDTDEAKEPKEEPIETEKPRAARPSFKFNICDGGFTELHSLWANEEKVARNGKEYEIWYRRHDYWLLAGVVVHGYGRFQANFNDIINDPRFSVLNEPFKEVGAEATGSDIKAKFMQRRFKLIEQSLVIEEQLRRAAHANRHLQPDNVGPLAQRFADLENIAESQANIAKESSAGNRNANAVLHKTLVQLDEILSDMKADVSRLPSTFTQLATVTERLNMTERQILSRLTTKDEDAIANRSVLPPPGPFVTPILRQQMDGIQPKFAALYSKFMSENGERMEEDEPVEAEEEEGVKQEPDDETQDSAEAPPVLSAEVNSDDSNDVPSTSAAAAVSSETAADAEPASAEDQAPTDEPEPMET

Target ORF information:

RefSeq Version	NM_072122.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein let-418 (let-418), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_072122.5

ORF Insert Sequence:

ATGTCAACTG AAGAAGATCC GTCTTTAGTG GACGCGGAAG AATCAATGGA AGAGGGTTCT 
GTCACTCAAG ACGCAACTGA AGAAACAGAA GAAGAGGAAG AACAAGAGCA AGGCGATGAG 
GCTGGACCAT CTGAGCGAAA GCGTTCTTCT AGAAAGAAAG GCGGAAAGGG AGGCAAAAAA 
GGATCCAAAA AGAGTGCTGC CGCAGCTAGC AAGGTTGAGA TTCCTGATCC GTATAATTCG 
ACAAGTGAAG AAGTTTGTGC TGCTATCGGA TTGACAGACG TCGAATTTGA TTATGATGAA 
GAGGAGTTTC AAGGAATTTC TAATCTGAAG ACTTTCTCCT CAATCATCAA GCCACAAATA 
CTGGAGGCAA ACCCCGGAAC CAACGTATCC AAGATGTACC CGATGTTCCA AGTCAAATAT 
AAAGAGTATC AAGATCACAT GGCTGCCCAA GGAAAACCTG TTCAAAAACA AGCTAGAGGT 
TCAAAGACTC CAGCAGTTTC CACACCCGTC ATCCCTCCAC GATCAGCTCC AACGAAGACT 
CGTTCTGCAA GGAGAAAGCG TCGAGATTCC GATGCTCCTG ATAGTGATCA AGAGTTTGAG 
GCATTTATTA AGCAACAAGA ACAACTGGAG GACGATTTGG TAAAAGACAA GGAAGATGCT 
CGTATCAAAC GTGCAGCGGA AAGAGAAGAG AAGAAAAAAG GAGCATTGGA AGCTGCTCGT 
GCCGCTAAAA AAGCAAAATT AGAAAAGGGC GAAGAAGCTG AAAATAATGA CTACTGTGAG 
GAGTGCAAGC AAGATGGAGA ACTTCTTCTT TGTGACACCT GTCCCCGCGC TTACCATACA 
GTCTGTATTG ATGAAAATAT GGAAGAACCA CCAGAGGGTG ATTGGAGTTG TGCTCATTGT 
ATCGAACATG GCCCAGAAGT AGTTAAAGAA GAACCGGCGA AGCAAAACGA TGAATTCTGC 
AAGATCTGCA AAGAAACTGA GAATCTTCTG CTTTGTGATA GTTGTGTATG CTCATTCCAC 
GCATATTGTA TTGATCCTCC ACTTACCGAA GTTCCGAAAG AAGAAACATG GTCGTGCCCA 
CGGTGCGAGA CGGTTAAGCC GGAACACAAA ATCGAAAAAA TCCTTTGCTG GAGATGGAAG 
GAAATCCCGT ATCCAGAACC TCTCGAAGCT GGGAAAGAGG CTTCGAGTGA CGATGCGATG 
CTCAAACCAC CACGGAAAAT GGAACCAAGA CGTGAGCGTG AATTCTTCGT GAAATGGAAG 
TATTTATCAT ACTGGCAGTG TTCTTGGGTC TCCGAAATGC TGTTGGAAGT TCATTTCCGT 
ATGCTTATAT TGCTATATTG GCGCAAAAAT GATTCCGATG CTCCTCCAGA ATTTGAAGAA 
TCCGTTACGT CTCGTCATCA TTCTGATAAT GATCCTTATA AATTGCGCGA GCGGTTCTAC 
CAGTATGGTA TCAAACCGGA ATGGATGCAA ATTCATCGTA TTATCAATCA TCAATCATAT 
GCAAAGTCGC AGCAGGATTA CCTTGTTAAG TGGAAGGAGC TCAGCTATGA TCAAGCCACT 
TGGGAACGAG ATGATTCTAA TATTGCCAAT TACGAAGAAG CAATCATTAA ATACTGGCAA 
CATCGTGAGA GCAAATTAAA TGAGGATATT CCTAAAAATG TGCAGAAGAT GATTGCTAAA 
CATCGCGAAG CTAAAGGTCT TCCTCCAAAA GAAGACGAAA AGAAAAAGAA GAAGCGTGAG 
AAGATTGACA TCCGTAAAAA ATATGAAGTT CAGCCCGACT ACGTTACGGA AACCGGAGGA 
AAACTTCATC CATATCAACT GGAAGGACTT AACTGGCTTC GTCATTGTTG GTCCAACGGA 
ACTGATGCGA TTCTCGCTGA TGAAATGGGT CTTGGGAAGA CTGTTCAATC GCTTACTTTC 
TTATACAGTC TCATGAAGGA AGGCCATTGC AAAGGACCAT TTTTGATTGC CGCTCCTCTA 
TCAACTATTA TCAATTGGGA GCGTGAGGCT GAACAATGGT GTCCTGATTT CTATGTGGTC 
ACTTATGTTG GTCTTAGAGA TGCTCGTGTT GTGCTCCGAG AACATGAGTT TTCTTTCGTT 
GAAGGTGCCG TTCGATCCGG ACCGAAGGCT TCAAAAATGA AGACGACAGA GAATATGAAG 
TTCCACGTGT TGCTCACTTC TTATGAGACT ATCAATATGG ATAAAACTAT ACTTTCTTCC 
ATTGAATGGG GAGCTCTTGT TGTTGATGAG GCTCATCGGT TAAAGAATAA TCAGTCGTTG 
TTCTTCAAAA ATCTCAATGA ATATACTATT CATTATCGCG TACTTCTCAC CGGAACTCCA 
CTTCAAAACA ATTTAGAAGA GCTCTTCCAT CTCCTCAATT TCTTGTCCAA AGAACGTTTC 
AATCAGCTCG AGGCTTTCAC TGCCGAATTC AATGAGATTT CCAAAGAGGA TCAAATTGAA 
AAACTTCATA ACTTGCTCGG ACCTCACATG CTTCGCCGTC TCAAAGCTGA TGTGTTGACT 
GGCATGCCAT CGAAATCAGA ATTAATTGTT CGAGTTGAAC TGAGTGCTAT GCAGAAAAAA 
TGGTATAAAA ATATTCTGAC TCGCAATTTT GATGCATTGA ATGTGAAGAA TGGAGGAACT 
CAAATGTCAT TGATGAACGT CCTTATGGAA TTGAAGAAAT GTTGTAATCA TCCATATCTT 
TTTGTGAAGG CAGAGTTGGA GGCTCCGAAA GAGAAAAACG GAATGTATGA AGGAACGGCG 
CTCATCAAGA ATTCCGGAAA GTTTGTTCTT CTTCAAAAAA TGTTGAGAAA GCTGAAGGAT 
GGTGGCCACC GAGTGCTTAT CTTCTCACAA ATGACAAGGA TGCTGGACAT AATGGAAGAT 
TTATGTGAAT ATGAAGGATA CAGATATGAA AGAATAGATG GAAGTATCAT GGGACAAATG 
AGACAAGATG CTATCGATCG TTACAATGCT CCTGGAGCTC AACAATTTAT TTTCTTGCTC 
TCCACTCGAG CTGGAGGTCT TGGTATCAAT CTGGCAACTG CTGATACGGT TATCATATAT 
GATTCTGATT GGAATCCTCA CAATGATATT CAAGCTTTCT CTCGTGCACA TCGTCTCGGA 
CAGAAGCATA AAGTTATGAT TTATCGTTTT GTAACAAAGA AATCTGTCGA AGAAAAAATC 
ACTTCAGTTG CCAAGAAGAA GATGCTTTTG AATCACTTGG TTGTACGTGC AGGATTGGGA 
GGAAAAGAAG GAAAGACAAT GAGCAAGACT GAGCTGGACG ATGTGCTTCG ATGGGGAACC 
GAAGAGTTAT TCAGTGAGGA TCTTGATGCT GCCGAAGGCG AAGGCTCGGA GAAGAAAGGT 
GCTGCCGCAC AAGAAATTGT TTGGGATGAT GCAGCTGTAG ATGCTCTTCT CGATCGCAGT 
AACAAAGAAG AGACTCCAGC AGGAGAAGAT GGCGAAGAAA AAGCTGAATG GCAAAACGAA 
TATCTAAGCT CGTTCAAGGT GGCCAGCTAT CAGACAAAAG AGACTGAAGG ACAAGAAGAA 
GAGGAGGAAG AGGAAACGGA AGTGATTAAA GAAGATGAGA AAGAACCAGA TCCAGATTAT 
TGGGAGAAGC TGCTCAAGCA TCACTACGAA CAGGACAGGG AAATCGAATT GCAAAAGCTT 
GGAAAGGGAA AACGTGTTCG CAAACAAATC AACTATGCCA GTGAGAATAT GGGAACTGAT 
TGGAGCAAGC AGAACCAAAC CCAAGATGAT GACGATGATA ACGAGTCGTA CCGTGGTTCG 
GACAATGGCG ACGGCCTGAA CTCTGATGAA GATGATTACG ATGAGAAGAA AAAGCGGCGT 
CGTGATGAAG AAAAAATGCC TCCACTCATG GCTAAAGTCA ATGGACAAGT TGAGATTCTT 
GGTTTCAATC CACGTCAACG CAAGGCGTTC TACGGGGCTG TTATGCGCTG GGGAATGCCT 
CCACAAGATT CTCATCAATC TCAATGGCTT GTTCGAGATC TGAGAAACAA ATCGGAAAAA 
GTCTTCCGAG CTTATGCTTC ACTGTTCATG CGACATCTCT GTGAGCCTGG AGCTGATGGT 
CATGATACCT TTAATGATGG AGTTCCAAGA GAAGGTCTTA ATCGTCAGCA TGTTCTTGGC 
CGAATCGGAT TGCTGAGTTT AGTTCGGCGG AAGGTTCAAG AATTTGAGCA ATATAATGGC 
CAATGGTCTA TGCCTGAAAT ACAAGACGAA GTTCTCGCTA AAGCTGCCAA TGGTTCGGCA 
CAAGGATCTT CAAGAAGTAC TCCGAAACCA AAAGAAGAGC CGAAGGAAGA ACCAATGGAA 
AAGGAAGATG CTACTGAAAC AGTTAACGGA GCTACATCAG AGCCAGCTAC AGATGCTGAG 
AGTGAACAAA ACGCTCCTGT TGACGAACCA ATGGATACAG ATGAAGCCAA AGAACCGAAA 
GAAGAACCAA TTGAAACCGA GAAACCAAGA GCAGCCCGTC CATCATTCAA GTTCAACATT 
TGTGACGGTG GATTCACTGA ACTTCACTCT TTGTGGGCCA ATGAAGAGAA AGTTGCAAGA 
AACGGAAAAG AATATGAAAT CTGGTACCGC AGACACGACT ATTGGCTTTT AGCTGGTGTT 
GTTGTACACG GATATGGACG TTTCCAAGCT AATTTCAATG ACATCATCAA TGATCCCAGA 
TTCTCAGTTC TCAATGAGCC ATTCAAGGAA GTTGGCGCTG AGGCAACAGG ATCTGACATC 
AAAGCCAAGT TTATGCAACG TCGTTTCAAA CTTATTGAAC AGTCCCTTGT GATTGAGGAG 
CAACTTCGTC GCGCTGCACA CGCCAATCGT CACTTGCAGC CGGATAATGT TGGACCATTG 
GCTCAACGTT TTGCGGATTT AGAGAATATT GCTGAATCTC AAGCTAATAT CGCCAAAGAA 
AGTTCTGCTG GAAATCGTAA TGCGAATGCC GTTCTACACA AAACATTGGT TCAGCTCGAC 
GAAATACTTT CCGATATGAA GGCCGACGTG TCTAGACTTC CATCTACTTT TACACAGTTA 
GCGACTGTCA CCGAACGTCT CAACATGACC GAGAGACAAA TTCTTTCGCG ACTGACCACA 
AAAGACGAGG ACGCGATAGC CAACCGTAGC GTACTTCCAC CACCAGGACC ATTTGTAACT 
CCAATACTTC GCCAGCAGAT GGATGGCATT CAACCAAAGT TTGCAGCTCT TTACAGTAAA 
TTCATGTCTG AAAATGGAGA AAGGATGGAA GAGGATGAAC CAGTAGAGGC AGAAGAGGAA 
GAAGGTGTAA AACAAGAACC AGATGATGAG ACTCAAGATA GCGCCGAAGC TCCGCCAGTT 
CTTAGTGCAG AAGTGAATTC CGACGATAGC AACGATGTTC CATCTACTTC CGCTGCTGCT 
GCTGTCAGTT CTGAAACAGC TGCCGACGCC GAACCAGCTT CCGCCGAAGA TCAAGCTCCA 
ACAGATGAGC CAGAACCAAT GGAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-418 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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