egl-8 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	egl-8
Entrez Gene ID	178537
Full Name	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001026175.3	NP_001021346.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
NM_001313089.1	NP_001300018.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313097.1	NP_001300026.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313105.1	NP_001300034.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313093.2	NP_001300022.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372983.1	NP_001360392.1	Phosphoinositide phospholipase C
NM_001313097.2	NP_001300026.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313099.2	NP_001300028.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313105.2	NP_001300034.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001026176.2	NP_001021347.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
NM_001313085.1	NP_001300014.1	Phosphoinositide phospholipase C
NM_001313087.1	NP_001300016.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313092.1	NP_001300021.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313093.1	NP_001300022.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313096.1	NP_001300025.1	Phosphoinositide phospholipase C
NM_001313098.1	NP_001300027.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313100.1	NP_001300029.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313101.1	NP_001300030.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313102.1	NP_001300031.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313103.1	NP_001300032.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313104.1	NP_001300033.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313106.1	NP_001300035.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001026175.4	NP_001021346.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
NM_001313085.2	NP_001300014.1	Phosphoinositide phospholipase C
NM_001313091.2	NP_001300020.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372982.1	NP_001360391.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372987.1	NP_001360396.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313086.1	NP_001300015.1	Phosphoinositide phospholipase C
NM_001313090.1	NP_001300019.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313095.1	NP_001300024.1	Phosphoinositide phospholipase C
NM_001026177.4	NP_001021348.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
NM_001372978.1	NP_001360387.1	Phosphoinositide phospholipase C
NM_001313101.2	NP_001300030.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372988.1	NP_001360397.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313091.1	NP_001300020.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313099.1	NP_001300028.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372980.1	NP_001360389.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372981.1	NP_001360390.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313103.2	NP_001300032.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001026177.3	NP_001021348.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
NM_001313088.1	NP_001300017.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313094.1	NP_001300023.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001026176.3	NP_001021347.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
NM_001313087.2	NP_001300016.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372979.1	NP_001360388.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313089.2	NP_001300018.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001313095.2	NP_001300024.1	Phosphoinositide phospholipase C
NM_001372984.1	NP_001360393.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372985.1	NP_001360394.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
NM_001372986.1	NP_001360395.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Pathways from BioSystems

Homologies

	PLCB4	XP_002798260.1
Canis lupus familiaris (dog)	PLCB4	XP_859719.2
Bos taurus (cattle)	PLCB4	NP_001159982.1
Mus musculus (house mouse)	Plcb4	NP_038857.1
Rattus norvegicus (Norway rat)	Plcb4	NP_077329.1
Gallus gallus (chicken)	PLCB4	NP_001186364.1
Danio rerio (zebrafish)	LOC100537538	XP_003200348.2
Homo sapiens (human)	PLCB4	NP_877949.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001936	XP_003435972.1
Drosophila melanogaster (fruit fly)	norpA	NP_001014720.1
Xenopus tropicalis (tropical clawed frog)	plcb4	XP_004914780.1
Pan troglodytes (chimpanzee)	PLCB4	XP_001168348.2
Caenorhabditis elegans (roundworm)	egl-8	NP_001021346.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Trio's Rho-specific GEF domain (unc-73)is a major Galpha(q) effector that, together with PLCbeta, mediates the Galpha(q) signaling that drives the locomotion, egg laying, and growth.
Title: Trio's Rho-specific GEF domain is the missing Galpha q effector in C. elegans.

The following egl-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egl-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa346705	NM_001026177.4 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa346703	NM_001026175.4 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa346704	NM_001026176.3 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348021	NM_001313087.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348022	NM_001372979.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348022	NM_001313088.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348023	NM_001313089.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348024	NM_001372980.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348024	NM_001313090.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348025	NM_001313091.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348026	NM_001313092.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348026	NM_001372981.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348027	NM_001313093.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348028	NM_001313094.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348028	NM_001372982.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348029	NM_001313095.2 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348030	NM_001372983.1 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348030	NM_001313096.1 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348031	NM_001313097.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348032	NM_001313098.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348032	NM_001372984.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348033	NM_001313099.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348034	NM_001372985.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa348034	NM_001313100.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348035	NM_001313101.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348036	NM_001313102.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348036	NM_001372986.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348037	NM_001313103.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348038	NM_001313104.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348038	NM_001372987.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348039	NM_001313105.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348040	NM_001313106.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348040	NM_001372988.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa348019	NM_001313085.2 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa348020	NM_001372978.1 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa348020	NM_001313086.1 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OCa348031	NM_001313097.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348039	NM_001313105.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348027	NM_001313093.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa346703	NM_001026175.3	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348019	NM_001313085.1	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa348035	NM_001313101.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348025	NM_001313091.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348033	NM_001313099.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348037	NM_001313103.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa346705	NM_001026177.3	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa346704	NM_001026176.2	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OCa348021	NM_001313087.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa348023	NM_001313089.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa348029	NM_001313095.1	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egl-8 ( NM_001026177.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021348.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.

Price & Availability↑

CloneID	OCa346705
Clone ID Related Accession (Same CDS sequence)	NM_001026177.4 , NM_001026177.3
Accession Version	NM_001026177.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001026177.3.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AAGAAACAAA CAAAATTTCA AATTTTAACA TAGTTTTTAA GGGTTTCCTC 
TTCCTCCTTA TTCTTATTCT TCTCTCTCAA ATCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021348.1
CDS	183..1961
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQETNKISNFNIVFKGFLFLLILILLSQIP

Target ORF information:

RefSeq Version	NM_001026177.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026177.4

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AAGAAACAAA CAAAATTTCA AATTTTAACA TAGTTTTTAA GGGTTTCCTC 
TTCCTCCTTA TTCTTATTCT TCTCTCTCAA ATCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001026177.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021348.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa346705
Clone ID Related Accession (Same CDS sequence)	NM_001026177.4 , NM_001026177.3
Accession Version	NM_001026177.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_001026177.2. COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AAGAAACAAA CAAAATTTCA AATTTTAACA TAGTTTTTAA GGGTTTCCTC 
TTCCTCCTTA TTCTTATTCT TCTCTCTCAA ATCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021348.1
CDS	1..1779
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQETNKISNFNIVFKGFLFLLILILLSQIP

Target ORF information:

RefSeq Version	NM_001026177.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026177.3

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AAGAAACAAA CAAAATTTCA AATTTTAACA TAGTTTTTAA GGGTTTCCTC 
TTCCTCCTTA TTCTTATTCT TCTCTCTCAA ATCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001026176.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021347.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa346704
Clone ID Related Accession (Same CDS sequence)	NM_001026176.2 , NM_001026176.3
Accession Version	NM_001026176.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_001026176.1. COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCC GGCTCGCCGC CGTAATTCGT CCATAGCGTG GATCCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021347.1
CDS	1..4296
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001026176.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026176.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCC GGCTCGCCGC CGTAATTCGT CCATAGCGTG GATCCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001026175.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021346.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.

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CloneID	OCa346703
Clone ID Related Accession (Same CDS sequence)	NM_001026175.3 , NM_001026175.4
Accession Version	NM_001026175.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_001026175.2. COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCA AACCAACGTC GACAAGCTCA TCACAAATAA CCGACGTTCC 
ACGAAAAAAG AAAAAGGCTC AAGGAGATCC CTGACGGCTT CAGTATCATC TGGATGTGGA 
AGTGCATCTG GAACTGTGAC TGTCAGTGTT TGCAGTCCTT CTGGCGCGAG TTGCAGTGGG 
TACTCCACTG GGGGTCCTTC GACACCGGTG GCATGTAACT CTGATGGTAC CGGGTCTCCA 
GCGACTATTG GAAGTCCAGT GCCACAAGAT TTGGTCAATA ATGATAGGGT CCGATCTCTC 
GTGAACACTC AAACCGGAGA ATGGTCCGCT ATGGTCCGTA GACATGACGA GGAAGAGTTT 
GAGCTGAAAA AAGTACAGCT CAAAGAGCAA TTCGATCTGC TAAGAAAACT CATGAGTGAA 
GCTCAAAAGA ACCAAATGTT GGCTCTGAAG CTCCGGTTAG AAGCCGAAGG GAAAGATCTA 
AAGCAGACGC AGACGAAAAA AAGCATGGAA GATGCGAAAG TTATTCAATT GGACAAAGGT 
ATTAAGACCA AGGCAGAACG TGATCGTCGT GTCAAGGAAC TCAACGAGAA GAACCTGAAA 
ATGTTTGTGG AGGAGAGGAA GAGATTAGCC ATGAAAGCCC AAAAGCACGA AGAGCAACTC 
ACAAAACGAC ATCTCGACCA GCTCGAGCAA CTAGACAAGG ATTTCCATAA GGCCTTGGAT 
GCGGAGGTCG GCAACTACAA AGAAGAGCAA CTGGCTGCTC AACCGACTTC TGTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021346.1
CDS	1..4260
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001026175.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026175.3

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCA AACCAACGTC GACAAGCTCA TCACAAATAA CCGACGTTCC 
ACGAAAAAAG AAAAAGGCTC AAGGAGATCC CTGACGGCTT CAGTATCATC TGGATGTGGA 
AGTGCATCTG GAACTGTGAC TGTCAGTGTT TGCAGTCCTT CTGGCGCGAG TTGCAGTGGG 
TACTCCACTG GGGGTCCTTC GACACCGGTG GCATGTAACT CTGATGGTAC CGGGTCTCCA 
GCGACTATTG GAAGTCCAGT GCCACAAGAT TTGGTCAATA ATGATAGGGT CCGATCTCTC 
GTGAACACTC AAACCGGAGA ATGGTCCGCT ATGGTCCGTA GACATGACGA GGAAGAGTTT 
GAGCTGAAAA AAGTACAGCT CAAAGAGCAA TTCGATCTGC TAAGAAAACT CATGAGTGAA 
GCTCAAAAGA ACCAAATGTT GGCTCTGAAG CTCCGGTTAG AAGCCGAAGG GAAAGATCTA 
AAGCAGACGC AGACGAAAAA AAGCATGGAA GATGCGAAAG TTATTCAATT GGACAAAGGT 
ATTAAGACCA AGGCAGAACG TGATCGTCGT GTCAAGGAAC TCAACGAGAA GAACCTGAAA 
ATGTTTGTGG AGGAGAGGAA GAGATTAGCC ATGAAAGCCC AAAAGCACGA AGAGCAACTC 
ACAAAACGAC ATCTCGACCA GCTCGAGCAA CTAGACAAGG ATTTCCATAA GGCCTTGGAT 
GCGGAGGTCG GCAACTACAA AGAAGAGCAA CTGGCTGCTC AACCGACTTC TGTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001026175.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021346.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.

Price & Availability↑

CloneID	OCa346703
Clone ID Related Accession (Same CDS sequence)	NM_001026175.3 , NM_001026175.4
Accession Version	NM_001026175.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001026175.3.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCA AACCAACGTC GACAAGCTCA TCACAAATAA CCGACGTTCC 
ACGAAAAAAG AAAAAGGCTC AAGGAGATCC CTGACGGCTT CAGTATCATC TGGATGTGGA 
AGTGCATCTG GAACTGTGAC TGTCAGTGTT TGCAGTCCTT CTGGCGCGAG TTGCAGTGGG 
TACTCCACTG GGGGTCCTTC GACACCGGTG GCATGTAACT CTGATGGTAC CGGGTCTCCA 
GCGACTATTG GAAGTCCAGT GCCACAAGAT TTGGTCAATA ATGATAGGGT CCGATCTCTC 
GTGAACACTC AAACCGGAGA ATGGTCCGCT ATGGTCCGTA GACATGACGA GGAAGAGTTT 
GAGCTGAAAA AAGTACAGCT CAAAGAGCAA TTCGATCTGC TAAGAAAACT CATGAGTGAA 
GCTCAAAAGA ACCAAATGTT GGCTCTGAAG CTCCGGTTAG AAGCCGAAGG GAAAGATCTA 
AAGCAGACGC AGACGAAAAA AAGCATGGAA GATGCGAAAG TTATTCAATT GGACAAAGGT 
ATTAAGACCA AGGCAGAACG TGATCGTCGT GTCAAGGAAC TCAACGAGAA GAACCTGAAA 
ATGTTTGTGG AGGAGAGGAA GAGATTAGCC ATGAAAGCCC AAAAGCACGA AGAGCAACTC 
ACAAAACGAC ATCTCGACCA GCTCGAGCAA CTAGACAAGG ATTTCCATAA GGCCTTGGAT 
GCGGAGGTCG GCAACTACAA AGAAGAGCAA CTGGCTGCTC AACCGACTTC TGTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021346.1
CDS	183..4442
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001026175.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026175.4

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCA AACCAACGTC GACAAGCTCA TCACAAATAA CCGACGTTCC 
ACGAAAAAAG AAAAAGGCTC AAGGAGATCC CTGACGGCTT CAGTATCATC TGGATGTGGA 
AGTGCATCTG GAACTGTGAC TGTCAGTGTT TGCAGTCCTT CTGGCGCGAG TTGCAGTGGG 
TACTCCACTG GGGGTCCTTC GACACCGGTG GCATGTAACT CTGATGGTAC CGGGTCTCCA 
GCGACTATTG GAAGTCCAGT GCCACAAGAT TTGGTCAATA ATGATAGGGT CCGATCTCTC 
GTGAACACTC AAACCGGAGA ATGGTCCGCT ATGGTCCGTA GACATGACGA GGAAGAGTTT 
GAGCTGAAAA AAGTACAGCT CAAAGAGCAA TTCGATCTGC TAAGAAAACT CATGAGTGAA 
GCTCAAAAGA ACCAAATGTT GGCTCTGAAG CTCCGGTTAG AAGCCGAAGG GAAAGATCTA 
AAGCAGACGC AGACGAAAAA AAGCATGGAA GATGCGAAAG TTATTCAATT GGACAAAGGT 
ATTAAGACCA AGGCAGAACG TGATCGTCGT GTCAAGGAAC TCAACGAGAA GAACCTGAAA 
ATGTTTGTGG AGGAGAGGAA GAGATTAGCC ATGAAAGCCC AAAAGCACGA AGAGCAACTC 
ACAAAACGAC ATCTCGACCA GCTCGAGCAA CTAGACAAGG ATTTCCATAA GGCCTTGGAT 
GCGGAGGTCG GCAACTACAA AGAAGAGCAA CTGGCTGCTC AACCGACTTC TGTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001026176.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021347.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.

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CloneID	OCa346704
Clone ID Related Accession (Same CDS sequence)	NM_001026176.2 , NM_001026176.3
Accession Version	NM_001026176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001026176.2.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCC GGCTCGCCGC CGTAATTCGT CCATAGCGTG GATCCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021347.1
CDS	183..4478
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001026176.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026176.3

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCGGTACTGA CAAAAGAAGA AGAGGAGCGG 
ATTTTCGCCG AGTATCATTA TACCGGGGCA ACTACGAATA TACATCCGTT ACTCTCTTCA 
CTGGTCAATT ATACACATCC TGTCAAGTTT TCTGGATTTG ACGTTGCGGA AGCCAACAAC 
CTGCACTTCC ACATGTCCTC ATTTTCTGAG TCAACCGGTC TCGGCTACCT AAAACAATCG 
GCGCCGGAAT TTGTGAACTA CAACAAACGA CAATCGAGTA GAATTTATCC GAAAGGAGCC 
AGAGTTGATT CTTCCAATTT TTTGCCGCAA ATTTTCTGGA ACGCCGGATG TCAAATGGTG 
TCGCTCAACT TTCAAACTCC CGATGTCTAC ATGCAATTGA ACATGGGAAA ATTCGAGTAC 
AACGGTGGCT CGGGGTATCT GCTAAAACCA GATTTCCTCC GAAGACCCGA CAGAACATTC 
GACCCATTTT CCGAGAGCCC GGTGGACGGA GTGATTGCAG CACATTGTAG TGTTCGTGTC 
ATCTCGGGTC AATTCCTATC GGATCGCAAA ATCGGGACAT ACGTTGAGGT GGAAATGTAC 
GGACTACCTA CTGACACTAT TCGTAAGGAG CACAAGACGA AGGTAATACC CGGGAACGGA 
CTGAATCCAG TTTACAACGA GGATCCGTTT GTCTTCCGAA AAGTTGTGCT CCCCGAGCTC 
GCCGTGCTCC GGTTCGCCGT CTACGATGAG AACGGCAAGC AGCTGGGTCA GCGGATTCTT 
CCGCTGGATG GGCTTCAGGC AGGATATCGG CATATTTCGC TGAGGTCTGA CACGAATCAG 
AGCTTCATTC TGTCACCTGT GCTCTTTGTG CAAATTGTGA TCAAGACTTA TGTGCCGGAT 
GAGCTCAGTG GCCTCGTCGA TGCTCTCGCC GACCCACGAG CATTCCTCTC GGAGCAGAAA 
AAAAGGCAGG AAGCCCTGGC TCACATGGGT GTTGACGATA GTGATATTCC TGATGTGCCC 
AACACCAGGA ATATGGCTCT CCGACACGTG AAACAACCTC CAAGGCAGAA CGGATCGTCA 
GCTGATCTGC TGGCAAACAA CGGCCAAACT GGGTCGGCTA GGGGTGATCA GACCAGCTCG 
ATGGCTTCAA GTACTATTCG GTCACCCAAC GAACAACCGC AGCCAGTGGC TGTGGATAAA 
TTTAAGGTTG ACCCAATCGA AGTTGACGAT TTACGACGAG ACAAAGCTTT TGCAAAATTG 
CTCAAGAGAT TTCAAAAAGA GTTGGATGAT CTCAGGAAGA AACATCAGAA GCAGAGGGAT 
AGCATTCAAA AACAGCAGCC GGCTCGCCGC CGTAATTCGT CCATAGCGTG GATCCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313087.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300016.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348021
Clone ID Related Accession (Same CDS sequence)	NM_001313087.1 , NM_001313087.2
Accession Version	NM_001313087.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300016.1
CDS	1..4221
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313087.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313087.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313087.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300016.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348021
Clone ID Related Accession (Same CDS sequence)	NM_001313087.1 , NM_001313087.2
Accession Version	NM_001313087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313087.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300016.1
CDS	183..4403
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313087.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313087.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313088.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300017.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348022
Clone ID Related Accession (Same CDS sequence)	NM_001313088.1 , NM_001372979.1
Accession Version	NM_001313088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300017.1
CDS	1..4185
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313088.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313088.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372979.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360388.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348022
Clone ID Related Accession (Same CDS sequence)	NM_001313088.1 , NM_001372979.1
Accession Version	NM_001372979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360388.1
CDS	183..4367
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372979.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372979.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313089.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300018.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348023
Clone ID Related Accession (Same CDS sequence)	NM_001313089.1 , NM_001313089.2
Accession Version	NM_001313089.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300018.1
CDS	1..3978
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313089.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313089.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313089.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300018.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348023
Clone ID Related Accession (Same CDS sequence)	NM_001313089.1 , NM_001313089.2
Accession Version	NM_001313089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313089.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300018.1
CDS	183..4160
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313089.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313089.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313090.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300019.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348024
Clone ID Related Accession (Same CDS sequence)	NM_001313090.1 , NM_001372980.1
Accession Version	NM_001313090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300019.1
CDS	1..3942
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313090.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313090.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372980.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360389.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348024
Clone ID Related Accession (Same CDS sequence)	NM_001313090.1 , NM_001372980.1
Accession Version	NM_001372980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360389.1
CDS	183..4124
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372980.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372980.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCCAGGCG ATCAGCGTGC TCATCCGGAA 
GTCGAGCAAC CCGTGAGCTC ATCCTCTCCA GCCACACCGT CCATCAGCGG ACCCCCGCCG 
TGTGCCACGT CATCAGGTTC AACGTCATCA ATCACTATTA CCACAACGGG ATGCTCCACG 
TCATCGTCGG GACCCTCAAA ACATATTCTA GGGGGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313091.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300020.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348025
Clone ID Related Accession (Same CDS sequence)	NM_001313091.2 , NM_001313091.1
Accession Version	NM_001313091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313091.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300020.1
CDS	183..4223
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313091.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313091.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313091.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300020.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348025
Clone ID Related Accession (Same CDS sequence)	NM_001313091.2 , NM_001313091.1
Accession Version	NM_001313091.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300020.1
CDS	1..4041
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313091.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313091.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCCGGCTC GCCGCCGTAA TTCGTCCATA GCGTGGATCC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313092.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300021.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348026
Clone ID Related Accession (Same CDS sequence)	NM_001313092.1 , NM_001372981.1
Accession Version	NM_001313092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300021.1
CDS	1..4005
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313092.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313092.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372981.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360390.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348026
Clone ID Related Accession (Same CDS sequence)	NM_001313092.1 , NM_001372981.1
Accession Version	NM_001372981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360390.1
CDS	183..4187
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372981.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372981.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCTGATTC ATCCACTTCA TTGCTAACCG CATCACCAAC ACCGTCCTCT 
TCATCGATGG CCATGTCAAA TGCGAATAAT CCGTTCTCCT CCTCAACCCT TAACTCACCC 
CAACCAATGG AGAGGTCTCG AAGCGAAAAG CGATCGTTTC GACAGAAAAA GGGCGGCGTT 
TTCGGTGACG AACTCCACTC GGACTCGGCC GCACTTATCG ATTTTATGCG GACGGCGTCG 
AGCCGTAAGA AGAAGCCGGT ACTGACAAAA GAAGAAGAGG AGCGGATTTT CGCCGAGTAT 
CATTATACCG GGGCAACTAC GAATATACAT CCGTTACTCT CTTCACTGGT CAATTATACA 
CATCCTGTCA AGTTTTCTGG ATTTGACGTT GCGGAAGCCA ACAACCTGCA CTTCCACATG 
TCCTCATTTT CTGAGTCAAC CGGTCTCGGC TACCTAAAAC AATCGGCGCC GGAATTTGTG 
AACTACAACA AACGACAATC GAGTAGAATT TATCCGAAAG GAGCCAGAGT TGATTCTTCC 
AATTTTTTGC CGCAAATTTT CTGGAACGCC GGATGTCAAA TGGTGTCGCT CAACTTTCAA 
ACTCCCGATG TCTACATGCA ATTGAACATG GGAAAATTCG AGTACAACGG TGGCTCGGGG 
TATCTGCTAA AACCAGATTT CCTCCGAAGA CCCGACAGAA CATTCGACCC ATTTTCCGAG 
AGCCCGGTGG ACGGAGTGAT TGCAGCACAT TGTAGTGTTC GTGTCATCTC GGGTCAATTC 
CTATCGGATC GCAAAATCGG GACATACGTT GAGGTGGAAA TGTACGGACT ACCTACTGAC 
ACTATTCGTA AGGAGCACAA GACGAAGGTA ATACCCGGGA ACGGACTGAA TCCAGTTTAC 
AACGAGGATC CGTTTGTCTT CCGAAAAGTT GTGCTCCCCG AGCTCGCCGT GCTCCGGTTC 
GCCGTCTACG ATGAGAACGG CAAGCAGCTG GGTCAGCGGA TTCTTCCGCT GGATGGGCTT 
CAGGCAGGAT ATCGGCATAT TTCGCTGAGG TCTGACACGA ATCAGAGCTT CATTCTGTCA 
CCTGTGCTCT TTGTGCAAAT TGTGATCAAG ACTTATGTGC CGGATGAGCT CAGTGGCCTC 
GTCGATGCTC TCGCCGACCC ACGAGCATTC CTCTCGGAGC AGAAAAAAAG GCAGGAAGCC 
CTGGCTCACA TGGGTGTTGA CGATAGTGAT ATTCCTGATG TGCCCAACAC CAGGAATATG 
GCTCTCCGAC ACGTGAAACA ACCTCCAAGG CAGAACGGAT CGTCAGCTGA TCTGCTGGCA 
AACAACGGCC AAACTGGGTC GGCTAGGGGT GATCAGACCA GCTCGATGGC TTCAAGTACT 
ATTCGGTCAC CCAACGAACA ACCGCAGCCA GTGGCTGTGG ATAAATTTAA GGTTGACCCA 
ATCGAAGTTG ACGATTTACG ACGAGACAAA GCTTTTGCAA AATTGCTCAA GAGATTTCAA 
AAAGAGTTGG ATGATCTCAG GAAGAAACAT CAGAAGCAGA GGGATAGCAT TCAAAAACAG 
CAGCAAACCA ACGTCGACAA GCTCATCACA AATAACCGAC GTTCCACGAA AAAAGAAAAA 
GGCTCAAGGA GATCCCTGAC GGCTTCAGTA TCATCTGGAT GTGGAAGTGC ATCTGGAACT 
GTGACTGTCA GTGTTTGCAG TCCTTCTGGC GCGAGTTGCA GTGGGTACTC CACTGGGGGT 
CCTTCGACAC CGGTGGCATG TAACTCTGAT GGTACCGGGT CTCCAGCGAC TATTGGAAGT 
CCAGTGCCAC AAGATTTGGT CAATAATGAT AGGGTCCGAT CTCTCGTGAA CACTCAAACC 
GGAGAATGGT CCGCTATGGT CCGTAGACAT GACGAGGAAG AGTTTGAGCT GAAAAAAGTA 
CAGCTCAAAG AGCAATTCGA TCTGCTAAGA AAACTCATGA GTGAAGCTCA AAAGAACCAA 
ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC GAAGGGAAAG ATCTAAAGCA GACGCAGACG 
AAAAAAAGCA TGGAAGATGC GAAAGTTATT CAATTGGACA AAGGTATTAA GACCAAGGCA 
GAACGTGATC GTCGTGTCAA GGAACTCAAC GAGAAGAACC TGAAAATGTT TGTGGAGGAG 
AGGAAGAGAT TAGCCATGAA AGCCCAAAAG CACGAAGAGC AACTCACAAA ACGACATCTC 
GACCAGCTCG AGCAACTAGA CAAGGATTTC CATAAGGCCT TGGATGCGGA GGTCGGCAAC 
TACAAAGAAG AGCAACTGGC TGCTCAACCG ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313093.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300022.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348027
Clone ID Related Accession (Same CDS sequence)	NM_001313093.2 , NM_001313093.1
Accession Version	NM_001313093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313093.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300022.1
CDS	183..3980
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313093.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313093.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313093.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300022.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348027
Clone ID Related Accession (Same CDS sequence)	NM_001313093.2 , NM_001313093.1
Accession Version	NM_001313093.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300022.1
CDS	1..3798
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313093.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313093.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CCGGCTCGCC GCCGTAATTC GTCCATAGCG TGGATCCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372982.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360391.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348028
Clone ID Related Accession (Same CDS sequence)	NM_001372982.1 , NM_001313094.1
Accession Version	NM_001372982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360391.1
CDS	183..3944
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372982.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372982.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313094.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300023.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348028
Clone ID Related Accession (Same CDS sequence)	NM_001372982.1 , NM_001313094.1
Accession Version	NM_001313094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300023.1
CDS	1..3762
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313094.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313094.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGTGAAA TGCCCGCCAA GGAAAACGAC 
GAAGCACATC CGGAGCTAAA GCAGAATTTC ATTGCTAAAA ATTTGAAAGG ATTCGGGTTT 
TCGAAAAAAC AACCGGTACT GACAAAAGAA GAAGAGGAGC GGATTTTCGC CGAGTATCAT 
TATACCGGGG CAACTACGAA TATACATCCG TTACTCTCTT CACTGGTCAA TTATACACAT 
CCTGTCAAGT TTTCTGGATT TGACGTTGCG GAAGCCAACA ACCTGCACTT CCACATGTCC 
TCATTTTCTG AGTCAACCGG TCTCGGCTAC CTAAAACAAT CGGCGCCGGA ATTTGTGAAC 
TACAACAAAC GACAATCGAG TAGAATTTAT CCGAAAGGAG CCAGAGTTGA TTCTTCCAAT 
TTTTTGCCGC AAATTTTCTG GAACGCCGGA TGTCAAATGG TGTCGCTCAA CTTTCAAACT 
CCCGATGTCT ACATGCAATT GAACATGGGA AAATTCGAGT ACAACGGTGG CTCGGGGTAT 
CTGCTAAAAC CAGATTTCCT CCGAAGACCC GACAGAACAT TCGACCCATT TTCCGAGAGC 
CCGGTGGACG GAGTGATTGC AGCACATTGT AGTGTTCGTG TCATCTCGGG TCAATTCCTA 
TCGGATCGCA AAATCGGGAC ATACGTTGAG GTGGAAATGT ACGGACTACC TACTGACACT 
ATTCGTAAGG AGCACAAGAC GAAGGTAATA CCCGGGAACG GACTGAATCC AGTTTACAAC 
GAGGATCCGT TTGTCTTCCG AAAAGTTGTG CTCCCCGAGC TCGCCGTGCT CCGGTTCGCC 
GTCTACGATG AGAACGGCAA GCAGCTGGGT CAGCGGATTC TTCCGCTGGA TGGGCTTCAG 
GCAGGATATC GGCATATTTC GCTGAGGTCT GACACGAATC AGAGCTTCAT TCTGTCACCT 
GTGCTCTTTG TGCAAATTGT GATCAAGACT TATGTGCCGG ATGAGCTCAG TGGCCTCGTC 
GATGCTCTCG CCGACCCACG AGCATTCCTC TCGGAGCAGA AAAAAAGGCA GGAAGCCCTG 
GCTCACATGG GTGTTGACGA TAGTGATATT CCTGATGTGC CCAACACCAG GAATATGGCT 
CTCCGACACG TGAAACAACC TCCAAGGCAG AACGGATCGT CAGCTGATCT GCTGGCAAAC 
AACGGCCAAA CTGGGTCGGC TAGGGGTGAT CAGACCAGCT CGATGGCTTC AAGTACTATT 
CGGTCACCCA ACGAACAACC GCAGCCAGTG GCTGTGGATA AATTTAAGGT TGACCCAATC 
GAAGTTGACG ATTTACGACG AGACAAAGCT TTTGCAAAAT TGCTCAAGAG ATTTCAAAAA 
GAGTTGGATG ATCTCAGGAA GAAACATCAG AAGCAGAGGG ATAGCATTCA AAAACAGCAG 
CAAACCAACG TCGACAAGCT CATCACAAAT AACCGACGTT CCACGAAAAA AGAAAAAGGC 
TCAAGGAGAT CCCTGACGGC TTCAGTATCA TCTGGATGTG GAAGTGCATC TGGAACTGTG 
ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG AGTTGCAGTG GGTACTCCAC TGGGGGTCCT 
TCGACACCGG TGGCATGTAA CTCTGATGGT ACCGGGTCTC CAGCGACTAT TGGAAGTCCA 
GTGCCACAAG ATTTGGTCAA TAATGATAGG GTCCGATCTC TCGTGAACAC TCAAACCGGA 
GAATGGTCCG CTATGGTCCG TAGACATGAC GAGGAAGAGT TTGAGCTGAA AAAAGTACAG 
CTCAAAGAGC AATTCGATCT GCTAAGAAAA CTCATGAGTG AAGCTCAAAA GAACCAAATG 
TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA GGGAAAGATC TAAAGCAGAC GCAGACGAAA 
AAAAGCATGG AAGATGCGAA AGTTATTCAA TTGGACAAAG GTATTAAGAC CAAGGCAGAA 
CGTGATCGTC GTGTCAAGGA ACTCAACGAG AAGAACCTGA AAATGTTTGT GGAGGAGAGG 
AAGAGATTAG CCATGAAAGC CCAAAAGCAC GAAGAGCAAC TCACAAAACG ACATCTCGAC 
CAGCTCGAGC AACTAGACAA GGATTTCCAT AAGGCCTTGG ATGCGGAGGT CGGCAACTAC 
AAAGAAGAGC AACTGGCTGC TCAACCGACT TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313095.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300024.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348029
Clone ID Related Accession (Same CDS sequence)	NM_001313095.1 , NM_001313095.2
Accession Version	NM_001313095.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCCG 
GCTCGCCGCC GTAATTCGTC CATAGCGTGG ATCCAAACCA ACGTCGACAA GCTCATCACA 
AATAACCGAC GTTCCACGAA AAAAGAAAAA GGCTCAAGGA GATCCCTGAC GGCTTCAGTA 
TCATCTGGAT GTGGAAGTGC ATCTGGAACT GTGACTGTCA GTGTTTGCAG TCCTTCTGGC 
GCGAGTTGCA GTGGGTACTC CACTGGGGGT CCTTCGACAC CGGTGGCATG TAACTCTGAT 
GGTACCGGGT CTCCAGCGAC TATTGGAAGT CCAGTGCCAC AAGATTTGGT CAATAATGAT 
AGGGTCCGAT CTCTCGTGAA CACTCAAACC GGAGAATGGT CCGCTATGGT CCGTAGACAT 
GACGAGGAAG AGTTTGAGCT GAAAAAAGTA CAGCTCAAAG AGCAATTCGA TCTGCTAAGA 
AAACTCATGA GTGAAGCTCA AAAGAACCAA ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC 
GAAGGGAAAG ATCTAAAGCA GACGCAGACG AAAAAAAGCA TGGAAGATGC GAAAGTTATT 
CAATTGGACA AAGGTATTAA GACCAAGGCA GAACGTGATC GTCGTGTCAA GGAACTCAAC 
GAGAAGAACC TGAAAATGTT TGTGGAGGAG AGGAAGAGAT TAGCCATGAA AGCCCAAAAG 
CACGAAGAGC AACTCACAAA ACGACATCTC GACCAGCTCG AGCAACTAGA CAAGGATTTC 
CATAAGGCCT TGGATGCGGA GGTCGGCAAC TACAAAGAAG AGCAACTGGC TGCTCAACCG 
ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300024.1
CDS	1..4455
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313095.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313095.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCCG 
GCTCGCCGCC GTAATTCGTC CATAGCGTGG ATCCAAACCA ACGTCGACAA GCTCATCACA 
AATAACCGAC GTTCCACGAA AAAAGAAAAA GGCTCAAGGA GATCCCTGAC GGCTTCAGTA 
TCATCTGGAT GTGGAAGTGC ATCTGGAACT GTGACTGTCA GTGTTTGCAG TCCTTCTGGC 
GCGAGTTGCA GTGGGTACTC CACTGGGGGT CCTTCGACAC CGGTGGCATG TAACTCTGAT 
GGTACCGGGT CTCCAGCGAC TATTGGAAGT CCAGTGCCAC AAGATTTGGT CAATAATGAT 
AGGGTCCGAT CTCTCGTGAA CACTCAAACC GGAGAATGGT CCGCTATGGT CCGTAGACAT 
GACGAGGAAG AGTTTGAGCT GAAAAAAGTA CAGCTCAAAG AGCAATTCGA TCTGCTAAGA 
AAACTCATGA GTGAAGCTCA AAAGAACCAA ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC 
GAAGGGAAAG ATCTAAAGCA GACGCAGACG AAAAAAAGCA TGGAAGATGC GAAAGTTATT 
CAATTGGACA AAGGTATTAA GACCAAGGCA GAACGTGATC GTCGTGTCAA GGAACTCAAC 
GAGAAGAACC TGAAAATGTT TGTGGAGGAG AGGAAGAGAT TAGCCATGAA AGCCCAAAAG 
CACGAAGAGC AACTCACAAA ACGACATCTC GACCAGCTCG AGCAACTAGA CAAGGATTTC 
CATAAGGCCT TGGATGCGGA GGTCGGCAAC TACAAAGAAG AGCAACTGGC TGCTCAACCG 
ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313095.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300024.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348029
Clone ID Related Accession (Same CDS sequence)	NM_001313095.1 , NM_001313095.2
Accession Version	NM_001313095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313095.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCCG 
GCTCGCCGCC GTAATTCGTC CATAGCGTGG ATCCAAACCA ACGTCGACAA GCTCATCACA 
AATAACCGAC GTTCCACGAA AAAAGAAAAA GGCTCAAGGA GATCCCTGAC GGCTTCAGTA 
TCATCTGGAT GTGGAAGTGC ATCTGGAACT GTGACTGTCA GTGTTTGCAG TCCTTCTGGC 
GCGAGTTGCA GTGGGTACTC CACTGGGGGT CCTTCGACAC CGGTGGCATG TAACTCTGAT 
GGTACCGGGT CTCCAGCGAC TATTGGAAGT CCAGTGCCAC AAGATTTGGT CAATAATGAT 
AGGGTCCGAT CTCTCGTGAA CACTCAAACC GGAGAATGGT CCGCTATGGT CCGTAGACAT 
GACGAGGAAG AGTTTGAGCT GAAAAAAGTA CAGCTCAAAG AGCAATTCGA TCTGCTAAGA 
AAACTCATGA GTGAAGCTCA AAAGAACCAA ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC 
GAAGGGAAAG ATCTAAAGCA GACGCAGACG AAAAAAAGCA TGGAAGATGC GAAAGTTATT 
CAATTGGACA AAGGTATTAA GACCAAGGCA GAACGTGATC GTCGTGTCAA GGAACTCAAC 
GAGAAGAACC TGAAAATGTT TGTGGAGGAG AGGAAGAGAT TAGCCATGAA AGCCCAAAAG 
CACGAAGAGC AACTCACAAA ACGACATCTC GACCAGCTCG AGCAACTAGA CAAGGATTTC 
CATAAGGCCT TGGATGCGGA GGTCGGCAAC TACAAAGAAG AGCAACTGGC TGCTCAACCG 
ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300024.1
CDS	183..4637
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313095.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313095.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCCG 
GCTCGCCGCC GTAATTCGTC CATAGCGTGG ATCCAAACCA ACGTCGACAA GCTCATCACA 
AATAACCGAC GTTCCACGAA AAAAGAAAAA GGCTCAAGGA GATCCCTGAC GGCTTCAGTA 
TCATCTGGAT GTGGAAGTGC ATCTGGAACT GTGACTGTCA GTGTTTGCAG TCCTTCTGGC 
GCGAGTTGCA GTGGGTACTC CACTGGGGGT CCTTCGACAC CGGTGGCATG TAACTCTGAT 
GGTACCGGGT CTCCAGCGAC TATTGGAAGT CCAGTGCCAC AAGATTTGGT CAATAATGAT 
AGGGTCCGAT CTCTCGTGAA CACTCAAACC GGAGAATGGT CCGCTATGGT CCGTAGACAT 
GACGAGGAAG AGTTTGAGCT GAAAAAAGTA CAGCTCAAAG AGCAATTCGA TCTGCTAAGA 
AAACTCATGA GTGAAGCTCA AAAGAACCAA ATGTTGGCTC TGAAGCTCCG GTTAGAAGCC 
GAAGGGAAAG ATCTAAAGCA GACGCAGACG AAAAAAAGCA TGGAAGATGC GAAAGTTATT 
CAATTGGACA AAGGTATTAA GACCAAGGCA GAACGTGATC GTCGTGTCAA GGAACTCAAC 
GAGAAGAACC TGAAAATGTT TGTGGAGGAG AGGAAGAGAT TAGCCATGAA AGCCCAAAAG 
CACGAAGAGC AACTCACAAA ACGACATCTC GACCAGCTCG AGCAACTAGA CAAGGATTTC 
CATAAGGCCT TGGATGCGGA GGTCGGCAAC TACAAAGAAG AGCAACTGGC TGCTCAACCG 
ACTTCTGTCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372983.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360392.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

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CloneID	OCa348030
Clone ID Related Accession (Same CDS sequence)	NM_001372983.1 , NM_001313096.1
Accession Version	NM_001372983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360392.1
CDS	183..4601
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372983.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372983.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313096.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300025.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

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CloneID	OCa348030
Clone ID Related Accession (Same CDS sequence)	NM_001372983.1 , NM_001313096.1
Accession Version	NM_001313096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300025.1
CDS	1..4419
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313096.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313096.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCTG ATTCATCCAC TTCATTGCTA ACCGCATCAC CAACACCGTC CTCTTCATCG 
ATGGCCATGT CAAATGCGAA TAATCCGTTC TCCTCCTCAA CCCTTAACTC ACCCCAACCA 
ATGGAGAGGT CTCGAAGCGA AAAGCGATCG TTTCGACAGA AAAAGGGCGG CGTTTTCGGT 
GACGAACTCC ACTCGGACTC GGCCGCACTT ATCGATTTTA TGCGGACGGC GTCGAGCCGT 
AAGAAGAAGC CGGTACTGAC AAAAGAAGAA GAGGAGCGGA TTTTCGCCGA GTATCATTAT 
ACCGGGGCAA CTACGAATAT ACATCCGTTA CTCTCTTCAC TGGTCAATTA TACACATCCT 
GTCAAGTTTT CTGGATTTGA CGTTGCGGAA GCCAACAACC TGCACTTCCA CATGTCCTCA 
TTTTCTGAGT CAACCGGTCT CGGCTACCTA AAACAATCGG CGCCGGAATT TGTGAACTAC 
AACAAACGAC AATCGAGTAG AATTTATCCG AAAGGAGCCA GAGTTGATTC TTCCAATTTT 
TTGCCGCAAA TTTTCTGGAA CGCCGGATGT CAAATGGTGT CGCTCAACTT TCAAACTCCC 
GATGTCTACA TGCAATTGAA CATGGGAAAA TTCGAGTACA ACGGTGGCTC GGGGTATCTG 
CTAAAACCAG ATTTCCTCCG AAGACCCGAC AGAACATTCG ACCCATTTTC CGAGAGCCCG 
GTGGACGGAG TGATTGCAGC ACATTGTAGT GTTCGTGTCA TCTCGGGTCA ATTCCTATCG 
GATCGCAAAA TCGGGACATA CGTTGAGGTG GAAATGTACG GACTACCTAC TGACACTATT 
CGTAAGGAGC ACAAGACGAA GGTAATACCC GGGAACGGAC TGAATCCAGT TTACAACGAG 
GATCCGTTTG TCTTCCGAAA AGTTGTGCTC CCCGAGCTCG CCGTGCTCCG GTTCGCCGTC 
TACGATGAGA ACGGCAAGCA GCTGGGTCAG CGGATTCTTC CGCTGGATGG GCTTCAGGCA 
GGATATCGGC ATATTTCGCT GAGGTCTGAC ACGAATCAGA GCTTCATTCT GTCACCTGTG 
CTCTTTGTGC AAATTGTGAT CAAGACTTAT GTGCCGGATG AGCTCAGTGG CCTCGTCGAT 
GCTCTCGCCG ACCCACGAGC ATTCCTCTCG GAGCAGAAAA AAAGGCAGGA AGCCCTGGCT 
CACATGGGTG TTGACGATAG TGATATTCCT GATGTGCCCA ACACCAGGAA TATGGCTCTC 
CGACACGTGA AACAACCTCC AAGGCAGAAC GGATCGTCAG CTGATCTGCT GGCAAACAAC 
GGCCAAACTG GGTCGGCTAG GGGTGATCAG ACCAGCTCGA TGGCTTCAAG TACTATTCGG 
TCACCCAACG AACAACCGCA GCCAGTGGCT GTGGATAAAT TTAAGGTTGA CCCAATCGAA 
GTTGACGATT TACGACGAGA CAAAGCTTTT GCAAAATTGC TCAAGAGATT TCAAAAAGAG 
TTGGATGATC TCAGGAAGAA ACATCAGAAG CAGAGGGATA GCATTCAAAA ACAGCAGCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313097.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300026.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348031
Clone ID Related Accession (Same CDS sequence)	NM_001313097.1 , NM_001313097.2
Accession Version	NM_001313097.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCCGGCT 
CGCCGCCGTA ATTCGTCCAT AGCGTGGATC CAAACCAACG TCGACAAGCT CATCACAAAT 
AACCGACGTT CCACGAAAAA AGAAAAAGGC TCAAGGAGAT CCCTGACGGC TTCAGTATCA 
TCTGGATGTG GAAGTGCATC TGGAACTGTG ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG 
AGTTGCAGTG GGTACTCCAC TGGGGGTCCT TCGACACCGG TGGCATGTAA CTCTGATGGT 
ACCGGGTCTC CAGCGACTAT TGGAAGTCCA GTGCCACAAG ATTTGGTCAA TAATGATAGG 
GTCCGATCTC TCGTGAACAC TCAAACCGGA GAATGGTCCG CTATGGTCCG TAGACATGAC 
GAGGAAGAGT TTGAGCTGAA AAAAGTACAG CTCAAAGAGC AATTCGATCT GCTAAGAAAA 
CTCATGAGTG AAGCTCAAAA GAACCAAATG TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA 
GGGAAAGATC TAAAGCAGAC GCAGACGAAA AAAAGCATGG AAGATGCGAA AGTTATTCAA 
TTGGACAAAG GTATTAAGAC CAAGGCAGAA CGTGATCGTC GTGTCAAGGA ACTCAACGAG 
AAGAACCTGA AAATGTTTGT GGAGGAGAGG AAGAGATTAG CCATGAAAGC CCAAAAGCAC 
GAAGAGCAAC TCACAAAACG ACATCTCGAC CAGCTCGAGC AACTAGACAA GGATTTCCAT 
AAGGCCTTGG ATGCGGAGGT CGGCAACTAC AAAGAAGAGC AACTGGCTGC TCAACCGACT 
TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300026.1
CDS	1..4212
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313097.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313097.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCCGGCT 
CGCCGCCGTA ATTCGTCCAT AGCGTGGATC CAAACCAACG TCGACAAGCT CATCACAAAT 
AACCGACGTT CCACGAAAAA AGAAAAAGGC TCAAGGAGAT CCCTGACGGC TTCAGTATCA 
TCTGGATGTG GAAGTGCATC TGGAACTGTG ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG 
AGTTGCAGTG GGTACTCCAC TGGGGGTCCT TCGACACCGG TGGCATGTAA CTCTGATGGT 
ACCGGGTCTC CAGCGACTAT TGGAAGTCCA GTGCCACAAG ATTTGGTCAA TAATGATAGG 
GTCCGATCTC TCGTGAACAC TCAAACCGGA GAATGGTCCG CTATGGTCCG TAGACATGAC 
GAGGAAGAGT TTGAGCTGAA AAAAGTACAG CTCAAAGAGC AATTCGATCT GCTAAGAAAA 
CTCATGAGTG AAGCTCAAAA GAACCAAATG TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA 
GGGAAAGATC TAAAGCAGAC GCAGACGAAA AAAAGCATGG AAGATGCGAA AGTTATTCAA 
TTGGACAAAG GTATTAAGAC CAAGGCAGAA CGTGATCGTC GTGTCAAGGA ACTCAACGAG 
AAGAACCTGA AAATGTTTGT GGAGGAGAGG AAGAGATTAG CCATGAAAGC CCAAAAGCAC 
GAAGAGCAAC TCACAAAACG ACATCTCGAC CAGCTCGAGC AACTAGACAA GGATTTCCAT 
AAGGCCTTGG ATGCGGAGGT CGGCAACTAC AAAGAAGAGC AACTGGCTGC TCAACCGACT 
TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313097.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300026.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348031
Clone ID Related Accession (Same CDS sequence)	NM_001313097.1 , NM_001313097.2
Accession Version	NM_001313097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313097.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCCGGCT 
CGCCGCCGTA ATTCGTCCAT AGCGTGGATC CAAACCAACG TCGACAAGCT CATCACAAAT 
AACCGACGTT CCACGAAAAA AGAAAAAGGC TCAAGGAGAT CCCTGACGGC TTCAGTATCA 
TCTGGATGTG GAAGTGCATC TGGAACTGTG ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG 
AGTTGCAGTG GGTACTCCAC TGGGGGTCCT TCGACACCGG TGGCATGTAA CTCTGATGGT 
ACCGGGTCTC CAGCGACTAT TGGAAGTCCA GTGCCACAAG ATTTGGTCAA TAATGATAGG 
GTCCGATCTC TCGTGAACAC TCAAACCGGA GAATGGTCCG CTATGGTCCG TAGACATGAC 
GAGGAAGAGT TTGAGCTGAA AAAAGTACAG CTCAAAGAGC AATTCGATCT GCTAAGAAAA 
CTCATGAGTG AAGCTCAAAA GAACCAAATG TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA 
GGGAAAGATC TAAAGCAGAC GCAGACGAAA AAAAGCATGG AAGATGCGAA AGTTATTCAA 
TTGGACAAAG GTATTAAGAC CAAGGCAGAA CGTGATCGTC GTGTCAAGGA ACTCAACGAG 
AAGAACCTGA AAATGTTTGT GGAGGAGAGG AAGAGATTAG CCATGAAAGC CCAAAAGCAC 
GAAGAGCAAC TCACAAAACG ACATCTCGAC CAGCTCGAGC AACTAGACAA GGATTTCCAT 
AAGGCCTTGG ATGCGGAGGT CGGCAACTAC AAAGAAGAGC AACTGGCTGC TCAACCGACT 
TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300026.1
CDS	183..4394
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313097.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313097.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCCGGCT 
CGCCGCCGTA ATTCGTCCAT AGCGTGGATC CAAACCAACG TCGACAAGCT CATCACAAAT 
AACCGACGTT CCACGAAAAA AGAAAAAGGC TCAAGGAGAT CCCTGACGGC TTCAGTATCA 
TCTGGATGTG GAAGTGCATC TGGAACTGTG ACTGTCAGTG TTTGCAGTCC TTCTGGCGCG 
AGTTGCAGTG GGTACTCCAC TGGGGGTCCT TCGACACCGG TGGCATGTAA CTCTGATGGT 
ACCGGGTCTC CAGCGACTAT TGGAAGTCCA GTGCCACAAG ATTTGGTCAA TAATGATAGG 
GTCCGATCTC TCGTGAACAC TCAAACCGGA GAATGGTCCG CTATGGTCCG TAGACATGAC 
GAGGAAGAGT TTGAGCTGAA AAAAGTACAG CTCAAAGAGC AATTCGATCT GCTAAGAAAA 
CTCATGAGTG AAGCTCAAAA GAACCAAATG TTGGCTCTGA AGCTCCGGTT AGAAGCCGAA 
GGGAAAGATC TAAAGCAGAC GCAGACGAAA AAAAGCATGG AAGATGCGAA AGTTATTCAA 
TTGGACAAAG GTATTAAGAC CAAGGCAGAA CGTGATCGTC GTGTCAAGGA ACTCAACGAG 
AAGAACCTGA AAATGTTTGT GGAGGAGAGG AAGAGATTAG CCATGAAAGC CCAAAAGCAC 
GAAGAGCAAC TCACAAAACG ACATCTCGAC CAGCTCGAGC AACTAGACAA GGATTTCCAT 
AAGGCCTTGG ATGCGGAGGT CGGCAACTAC AAAGAAGAGC AACTGGCTGC TCAACCGACT 
TCTGTCGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313098.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300027.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348032
Clone ID Related Accession (Same CDS sequence)	NM_001313098.1 , NM_001372984.1
Accession Version	NM_001313098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300027.1
CDS	1..4176
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313098.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313098.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372984.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360393.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348032
Clone ID Related Accession (Same CDS sequence)	NM_001313098.1 , NM_001372984.1
Accession Version	NM_001372984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360393.1
CDS	183..4358
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372984.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372984.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GAAGCGGCGG GAGCGGTGGC ACCGGAGCCC CGGAAGAAGT GGATGATGAC 
ACGTCCGACG ACGACGATGA CCCGTCGGTT CAGACATCGC TGAACGTGAT GCGCACCATC 
CCGACCGTGA ACACCACATC GAACAACGGA TCGAATCGAT CGGCAAGAAG TTCACTGGAC 
ACCCCATCCC CATCCGGTGG GAGCCTGATG GTTCCAGATA GGGCAACATC GACAGCGACG 
TCGATCAAGA ACGCCGTGCT CGCGAGGTCT CCGAACTTCT CCTCGTTGAG ACAAAAGTTG 
TCGTTCAAGC GGAGACAATC ACCACTAGCA GGCGATCAGC GTGCTCATCC GGAAGTCGAG 
CAACCCGTGA GCTCATCCTC TCCAGCCACA CCGTCCATCA GCGGACCCCC GCCGTGTGCC 
ACGTCATCAG GTTCAACGTC ATCAATCACT ATTACCACAA CGGGATGCTC CACGTCATCG 
TCGGGACCCT CAAAACATAT TCTAGGGGGT GAAATGCCCG CCAAGGAAAA CGACGAAGCA 
CATCCGGAGC TAAAGCAGAA TTTCATTGCT AAAAATTTGA AAGGATTCGG GTTTTCGAAA 
AAACAACCGG TACTGACAAA AGAAGAAGAG GAGCGGATTT TCGCCGAGTA TCATTATACC 
GGGGCAACTA CGAATATACA TCCGTTACTC TCTTCACTGG TCAATTATAC ACATCCTGTC 
AAGTTTTCTG GATTTGACGT TGCGGAAGCC AACAACCTGC ACTTCCACAT GTCCTCATTT 
TCTGAGTCAA CCGGTCTCGG CTACCTAAAA CAATCGGCGC CGGAATTTGT GAACTACAAC 
AAACGACAAT CGAGTAGAAT TTATCCGAAA GGAGCCAGAG TTGATTCTTC CAATTTTTTG 
CCGCAAATTT TCTGGAACGC CGGATGTCAA ATGGTGTCGC TCAACTTTCA AACTCCCGAT 
GTCTACATGC AATTGAACAT GGGAAAATTC GAGTACAACG GTGGCTCGGG GTATCTGCTA 
AAACCAGATT TCCTCCGAAG ACCCGACAGA ACATTCGACC CATTTTCCGA GAGCCCGGTG 
GACGGAGTGA TTGCAGCACA TTGTAGTGTT CGTGTCATCT CGGGTCAATT CCTATCGGAT 
CGCAAAATCG GGACATACGT TGAGGTGGAA ATGTACGGAC TACCTACTGA CACTATTCGT 
AAGGAGCACA AGACGAAGGT AATACCCGGG AACGGACTGA ATCCAGTTTA CAACGAGGAT 
CCGTTTGTCT TCCGAAAAGT TGTGCTCCCC GAGCTCGCCG TGCTCCGGTT CGCCGTCTAC 
GATGAGAACG GCAAGCAGCT GGGTCAGCGG ATTCTTCCGC TGGATGGGCT TCAGGCAGGA 
TATCGGCATA TTTCGCTGAG GTCTGACACG AATCAGAGCT TCATTCTGTC ACCTGTGCTC 
TTTGTGCAAA TTGTGATCAA GACTTATGTG CCGGATGAGC TCAGTGGCCT CGTCGATGCT 
CTCGCCGACC CACGAGCATT CCTCTCGGAG CAGAAAAAAA GGCAGGAAGC CCTGGCTCAC 
ATGGGTGTTG ACGATAGTGA TATTCCTGAT GTGCCCAACA CCAGGAATAT GGCTCTCCGA 
CACGTGAAAC AACCTCCAAG GCAGAACGGA TCGTCAGCTG ATCTGCTGGC AAACAACGGC 
CAAACTGGGT CGGCTAGGGG TGATCAGACC AGCTCGATGG CTTCAAGTAC TATTCGGTCA 
CCCAACGAAC AACCGCAGCC AGTGGCTGTG GATAAATTTA AGGTTGACCC AATCGAAGTT 
GACGATTTAC GACGAGACAA AGCTTTTGCA AAATTGCTCA AGAGATTTCA AAAAGAGTTG 
GATGATCTCA GGAAGAAACA TCAGAAGCAG AGGGATAGCA TTCAAAAACA GCAGCAAACC 
AACGTCGACA AGCTCATCAC AAATAACCGA CGTTCCACGA AAAAAGAAAA AGGCTCAAGG 
AGATCCCTGA CGGCTTCAGT ATCATCTGGA TGTGGAAGTG CATCTGGAAC TGTGACTGTC 
AGTGTTTGCA GTCCTTCTGG CGCGAGTTGC AGTGGGTACT CCACTGGGGG TCCTTCGACA 
CCGGTGGCAT GTAACTCTGA TGGTACCGGG TCTCCAGCGA CTATTGGAAG TCCAGTGCCA 
CAAGATTTGG TCAATAATGA TAGGGTCCGA TCTCTCGTGA ACACTCAAAC CGGAGAATGG 
TCCGCTATGG TCCGTAGACA TGACGAGGAA GAGTTTGAGC TGAAAAAAGT ACAGCTCAAA 
GAGCAATTCG ATCTGCTAAG AAAACTCATG AGTGAAGCTC AAAAGAACCA AATGTTGGCT 
CTGAAGCTCC GGTTAGAAGC CGAAGGGAAA GATCTAAAGC AGACGCAGAC GAAAAAAAGC 
ATGGAAGATG CGAAAGTTAT TCAATTGGAC AAAGGTATTA AGACCAAGGC AGAACGTGAT 
CGTCGTGTCA AGGAACTCAA CGAGAAGAAC CTGAAAATGT TTGTGGAGGA GAGGAAGAGA 
TTAGCCATGA AAGCCCAAAA GCACGAAGAG CAACTCACAA AACGACATCT CGACCAGCTC 
GAGCAACTAG ACAAGGATTT CCATAAGGCC TTGGATGCGG AGGTCGGCAA CTACAAAGAA 
GAGCAACTGG CTGCTCAACC GACTTCTGTC GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313099.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300028.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348033
Clone ID Related Accession (Same CDS sequence)	NM_001313099.2 , NM_001313099.1
Accession Version	NM_001313099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313099.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300028.1
CDS	183..4319
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313099.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313099.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313099.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300028.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348033
Clone ID Related Accession (Same CDS sequence)	NM_001313099.2 , NM_001313099.1
Accession Version	NM_001313099.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300028.1
CDS	1..4137
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313099.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313099.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313100.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300029.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348034
Clone ID Related Accession (Same CDS sequence)	NM_001313100.1 , NM_001372985.1
Accession Version	NM_001313100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300029.1
CDS	1..4101
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313100.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313100.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372985.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360394.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348034
Clone ID Related Accession (Same CDS sequence)	NM_001313100.1 , NM_001372985.1
Accession Version	NM_001372985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360394.1
CDS	183..4283
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372985.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372985.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313101.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300030.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348035
Clone ID Related Accession (Same CDS sequence)	NM_001313101.1 , NM_001313101.2
Accession Version	NM_001313101.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300030.1
CDS	1..3894
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313101.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313101.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313101.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300030.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348035
Clone ID Related Accession (Same CDS sequence)	NM_001313101.1 , NM_001313101.2
Accession Version	NM_001313101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313101.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300030.1
CDS	183..4076
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313101.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313101.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313102.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300031.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348036
Clone ID Related Accession (Same CDS sequence)	NM_001313102.1 , NM_001372986.1
Accession Version	NM_001313102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300031.1
CDS	1..3858
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313102.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313102.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372986.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360395.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348036
Clone ID Related Accession (Same CDS sequence)	NM_001313102.1 , NM_001372986.1
Accession Version	NM_001372986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360395.1
CDS	183..4040
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPPGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372986.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372986.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC CAGGCGATCA GCGTGCTCAT CCGGAAGTCG AGCAACCCGT GAGCTCATCC 
TCTCCAGCCA CACCGTCCAT CAGCGGACCC CCGCCGTGTG CCACGTCATC AGGTTCAACG 
TCATCAATCA CTATTACCAC AACGGGATGC TCCACGTCAT CGTCGGGACC CTCAAAACAT 
ATTCTAGGGG GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313103.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300032.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348037
Clone ID Related Accession (Same CDS sequence)	NM_001313103.1 , NM_001313103.2
Accession Version	NM_001313103.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300032.1
CDS	1..3957
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313103.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313103.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313103.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300032.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348037
Clone ID Related Accession (Same CDS sequence)	NM_001313103.1 , NM_001313103.2
Accession Version	NM_001313103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313103.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300032.1
CDS	183..4139
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313103.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313103.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC CGGCTCGCCG CCGTAATTCG 
TCCATAGCGT GGATCCAAAC CAACGTCGAC AAGCTCATCA CAAATAACCG ACGTTCCACG 
AAAAAAGAAA AAGGCTCAAG GAGATCCCTG ACGGCTTCAG TATCATCTGG ATGTGGAAGT 
GCATCTGGAA CTGTGACTGT CAGTGTTTGC AGTCCTTCTG GCGCGAGTTG CAGTGGGTAC 
TCCACTGGGG GTCCTTCGAC ACCGGTGGCA TGTAACTCTG ATGGTACCGG GTCTCCAGCG 
ACTATTGGAA GTCCAGTGCC ACAAGATTTG GTCAATAATG ATAGGGTCCG ATCTCTCGTG 
AACACTCAAA CCGGAGAATG GTCCGCTATG GTCCGTAGAC ATGACGAGGA AGAGTTTGAG 
CTGAAAAAAG TACAGCTCAA AGAGCAATTC GATCTGCTAA GAAAACTCAT GAGTGAAGCT 
CAAAAGAACC AAATGTTGGC TCTGAAGCTC CGGTTAGAAG CCGAAGGGAA AGATCTAAAG 
CAGACGCAGA CGAAAAAAAG CATGGAAGAT GCGAAAGTTA TTCAATTGGA CAAAGGTATT 
AAGACCAAGG CAGAACGTGA TCGTCGTGTC AAGGAACTCA ACGAGAAGAA CCTGAAAATG 
TTTGTGGAGG AGAGGAAGAG ATTAGCCATG AAAGCCCAAA AGCACGAAGA GCAACTCACA 
AAACGACATC TCGACCAGCT CGAGCAACTA GACAAGGATT TCCATAAGGC CTTGGATGCG 
GAGGTCGGCA ACTACAAAGA AGAGCAACTG GCTGCTCAAC CGACTTCTGT CGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313104.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300033.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348038
Clone ID Related Accession (Same CDS sequence)	NM_001313104.1 , NM_001372987.1
Accession Version	NM_001313104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300033.1
CDS	1..3921
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313104.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313104.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372987.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360396.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348038
Clone ID Related Accession (Same CDS sequence)	NM_001313104.1 , NM_001372987.1
Accession Version	NM_001372987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360396.1
CDS	183..4103
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372987.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372987.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC TGATTCATCC 
ACTTCATTGC TAACCGCATC ACCAACACCG TCCTCTTCAT CGATGGCCAT GTCAAATGCG 
AATAATCCGT TCTCCTCCTC AACCCTTAAC TCACCCCAAC CAATGGAGAG GTCTCGAAGC 
GAAAAGCGAT CGTTTCGACA GAAAAAGGGC GGCGTTTTCG GTGACGAACT CCACTCGGAC 
TCGGCCGCAC TTATCGATTT TATGCGGACG GCGTCGAGCC GTAAGAAGAA GCCGGTACTG 
ACAAAAGAAG AAGAGGAGCG GATTTTCGCC GAGTATCATT ATACCGGGGC AACTACGAAT 
ATACATCCGT TACTCTCTTC ACTGGTCAAT TATACACATC CTGTCAAGTT TTCTGGATTT 
GACGTTGCGG AAGCCAACAA CCTGCACTTC CACATGTCCT CATTTTCTGA GTCAACCGGT 
CTCGGCTACC TAAAACAATC GGCGCCGGAA TTTGTGAACT ACAACAAACG ACAATCGAGT 
AGAATTTATC CGAAAGGAGC CAGAGTTGAT TCTTCCAATT TTTTGCCGCA AATTTTCTGG 
AACGCCGGAT GTCAAATGGT GTCGCTCAAC TTTCAAACTC CCGATGTCTA CATGCAATTG 
AACATGGGAA AATTCGAGTA CAACGGTGGC TCGGGGTATC TGCTAAAACC AGATTTCCTC 
CGAAGACCCG ACAGAACATT CGACCCATTT TCCGAGAGCC CGGTGGACGG AGTGATTGCA 
GCACATTGTA GTGTTCGTGT CATCTCGGGT CAATTCCTAT CGGATCGCAA AATCGGGACA 
TACGTTGAGG TGGAAATGTA CGGACTACCT ACTGACACTA TTCGTAAGGA GCACAAGACG 
AAGGTAATAC CCGGGAACGG ACTGAATCCA GTTTACAACG AGGATCCGTT TGTCTTCCGA 
AAAGTTGTGC TCCCCGAGCT CGCCGTGCTC CGGTTCGCCG TCTACGATGA GAACGGCAAG 
CAGCTGGGTC AGCGGATTCT TCCGCTGGAT GGGCTTCAGG CAGGATATCG GCATATTTCG 
CTGAGGTCTG ACACGAATCA GAGCTTCATT CTGTCACCTG TGCTCTTTGT GCAAATTGTG 
ATCAAGACTT ATGTGCCGGA TGAGCTCAGT GGCCTCGTCG ATGCTCTCGC CGACCCACGA 
GCATTCCTCT CGGAGCAGAA AAAAAGGCAG GAAGCCCTGG CTCACATGGG TGTTGACGAT 
AGTGATATTC CTGATGTGCC CAACACCAGG AATATGGCTC TCCGACACGT GAAACAACCT 
CCAAGGCAGA ACGGATCGTC AGCTGATCTG CTGGCAAACA ACGGCCAAAC TGGGTCGGCT 
AGGGGTGATC AGACCAGCTC GATGGCTTCA AGTACTATTC GGTCACCCAA CGAACAACCG 
CAGCCAGTGG CTGTGGATAA ATTTAAGGTT GACCCAATCG AAGTTGACGA TTTACGACGA 
GACAAAGCTT TTGCAAAATT GCTCAAGAGA TTTCAAAAAG AGTTGGATGA TCTCAGGAAG 
AAACATCAGA AGCAGAGGGA TAGCATTCAA AAACAGCAGC AAACCAACGT CGACAAGCTC 
ATCACAAATA ACCGACGTTC CACGAAAAAA GAAAAAGGCT CAAGGAGATC CCTGACGGCT 
TCAGTATCAT CTGGATGTGG AAGTGCATCT GGAACTGTGA CTGTCAGTGT TTGCAGTCCT 
TCTGGCGCGA GTTGCAGTGG GTACTCCACT GGGGGTCCTT CGACACCGGT GGCATGTAAC 
TCTGATGGTA CCGGGTCTCC AGCGACTATT GGAAGTCCAG TGCCACAAGA TTTGGTCAAT 
AATGATAGGG TCCGATCTCT CGTGAACACT CAAACCGGAG AATGGTCCGC TATGGTCCGT 
AGACATGACG AGGAAGAGTT TGAGCTGAAA AAAGTACAGC TCAAAGAGCA ATTCGATCTG 
CTAAGAAAAC TCATGAGTGA AGCTCAAAAG AACCAAATGT TGGCTCTGAA GCTCCGGTTA 
GAAGCCGAAG GGAAAGATCT AAAGCAGACG CAGACGAAAA AAAGCATGGA AGATGCGAAA 
GTTATTCAAT TGGACAAAGG TATTAAGACC AAGGCAGAAC GTGATCGTCG TGTCAAGGAA 
CTCAACGAGA AGAACCTGAA AATGTTTGTG GAGGAGAGGA AGAGATTAGC CATGAAAGCC 
CAAAAGCACG AAGAGCAACT CACAAAACGA CATCTCGACC AGCTCGAGCA ACTAGACAAG 
GATTTCCATA AGGCCTTGGA TGCGGAGGTC GGCAACTACA AAGAAGAGCA ACTGGCTGCT 
CAACCGACTT CTGTCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313105.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300034.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348039
Clone ID Related Accession (Same CDS sequence)	NM_001313105.1 , NM_001313105.2
Accession Version	NM_001313105.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300034.1
CDS	1..3714
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313105.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313105.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313105.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300034.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348039
Clone ID Related Accession (Same CDS sequence)	NM_001313105.1 , NM_001313105.2
Accession Version	NM_001313105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313105.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300034.1
CDS	183..3896
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313105.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313105.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCCGG CTCGCCGCCG TAATTCGTCC 
ATAGCGTGGA TCCAAACCAA CGTCGACAAG CTCATCACAA ATAACCGACG TTCCACGAAA 
AAAGAAAAAG GCTCAAGGAG ATCCCTGACG GCTTCAGTAT CATCTGGATG TGGAAGTGCA 
TCTGGAACTG TGACTGTCAG TGTTTGCAGT CCTTCTGGCG CGAGTTGCAG TGGGTACTCC 
ACTGGGGGTC CTTCGACACC GGTGGCATGT AACTCTGATG GTACCGGGTC TCCAGCGACT 
ATTGGAAGTC CAGTGCCACA AGATTTGGTC AATAATGATA GGGTCCGATC TCTCGTGAAC 
ACTCAAACCG GAGAATGGTC CGCTATGGTC CGTAGACATG ACGAGGAAGA GTTTGAGCTG 
AAAAAAGTAC AGCTCAAAGA GCAATTCGAT CTGCTAAGAA AACTCATGAG TGAAGCTCAA 
AAGAACCAAA TGTTGGCTCT GAAGCTCCGG TTAGAAGCCG AAGGGAAAGA TCTAAAGCAG 
ACGCAGACGA AAAAAAGCAT GGAAGATGCG AAAGTTATTC AATTGGACAA AGGTATTAAG 
ACCAAGGCAG AACGTGATCG TCGTGTCAAG GAACTCAACG AGAAGAACCT GAAAATGTTT 
GTGGAGGAGA GGAAGAGATT AGCCATGAAA GCCCAAAAGC ACGAAGAGCA ACTCACAAAA 
CGACATCTCG ACCAGCTCGA GCAACTAGAC AAGGATTTCC ATAAGGCCTT GGATGCGGAG 
GTCGGCAACT ACAAAGAAGA GCAACTGGCT GCTCAACCGA CTTCTGTCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313106.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300035.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

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CloneID	OCa348040
Clone ID Related Accession (Same CDS sequence)	NM_001313106.1 , NM_001372988.1
Accession Version	NM_001313106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300035.1
CDS	1..3678
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313106.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313106.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372988.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360397.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

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CloneID	OCa348040
Clone ID Related Accession (Same CDS sequence)	NM_001313106.1 , NM_001372988.1
Accession Version	NM_001372988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360397.1
CDS	183..3860
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372988.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372988.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGGAGCGCGA AGAACTTGAC 
GTCGACATCC TCACCTTTGA AAAATTCCAG AGGCTCTATA ACAAGATTTG TCCGAGAACC 
GAAGTTCAAG AGCTGTTTGT TAAATTATCC GGACAGAAAG AGTACCTAAC CAAAGAGCGA 
CTCATCAATT TTCTGAATGA AGAACAACGT GATCCACGAC TCAACGAGAT CCTCTTCCCA 
TTTTTTGATT CTCAGCGTAT CGTTGCGCTT CTCAAAAAAC ACGAAAACGA CATAAAATAT 
CAAGAAGATG GAAAAATGTC CGGTGACGGG TTTCTCCGAT TCCTGATGTC AGACGAGAAC 
CCGCCAGTGT TTCTAGACCG AATTGAAATG TTTATGGATA TGGATCAACC GCTGTGTCAC 
TACTACATCA ATAGTTCCCA TAACACGTAT CTTACCGGAC GGCAGTATGG TGGAAAATCG 
TCTTCTGAGA TTTATCGACA AGTTTTGCTC AGCGGATGTA GGTGTATTGA GCTTGATTGT 
TGGGATGGTA CTGGTGAAAA TAAAGGAGAA CCCATTATCA CTCACGGAAA GGCCATGTGC 
ACAGACGTGT TCTTCAAGGA CGTCCTGGTA CAAATTCGAG ACACCGCATT TGCCCGGTCG 
GATTTTCCAG TGGTCCTTTC CTTCGAAAAC CATTGCTCAA AATCAAATCA GCTGAAAATG 
GCCAAATATT GTATGGACAT TTTTGGAGAT ATGCTGCTGT CCAAACCGTT TGAAGATGCG 
CCTCTTGACC CCGGAGTCTC CCTACCTTCT CCAAACCGCC TGCGAAAGAA GATCCTTATC 
AAAAATAAGC GATTAAAAAC CGACATCGAA CGTCACCAAC TTGACCAATT TCTGCGGGAA 
GGCAAACTGG ACGAGGAGGA CGAGCTGAAT GAGACGCCCG AAGTTGTCGG AGAGGACTCC 
GTATCACCTC GTGAAATGCC CGCCAAGGAA AACGACGAAG CACATCCGGA GCTAAAGCAG 
AATTTCATTG CTAAAAATTT GAAAGGATTC GGGTTTTCGA AAAAACAACC GGTACTGACA 
AAAGAAGAAG AGGAGCGGAT TTTCGCCGAG TATCATTATA CCGGGGCAAC TACGAATATA 
CATCCGTTAC TCTCTTCACT GGTCAATTAT ACACATCCTG TCAAGTTTTC TGGATTTGAC 
GTTGCGGAAG CCAACAACCT GCACTTCCAC ATGTCCTCAT TTTCTGAGTC AACCGGTCTC 
GGCTACCTAA AACAATCGGC GCCGGAATTT GTGAACTACA ACAAACGACA ATCGAGTAGA 
ATTTATCCGA AAGGAGCCAG AGTTGATTCT TCCAATTTTT TGCCGCAAAT TTTCTGGAAC 
GCCGGATGTC AAATGGTGTC GCTCAACTTT CAAACTCCCG ATGTCTACAT GCAATTGAAC 
ATGGGAAAAT TCGAGTACAA CGGTGGCTCG GGGTATCTGC TAAAACCAGA TTTCCTCCGA 
AGACCCGACA GAACATTCGA CCCATTTTCC GAGAGCCCGG TGGACGGAGT GATTGCAGCA 
CATTGTAGTG TTCGTGTCAT CTCGGGTCAA TTCCTATCGG ATCGCAAAAT CGGGACATAC 
GTTGAGGTGG AAATGTACGG ACTACCTACT GACACTATTC GTAAGGAGCA CAAGACGAAG 
GTAATACCCG GGAACGGACT GAATCCAGTT TACAACGAGG ATCCGTTTGT CTTCCGAAAA 
GTTGTGCTCC CCGAGCTCGC CGTGCTCCGG TTCGCCGTCT ACGATGAGAA CGGCAAGCAG 
CTGGGTCAGC GGATTCTTCC GCTGGATGGG CTTCAGGCAG GATATCGGCA TATTTCGCTG 
AGGTCTGACA CGAATCAGAG CTTCATTCTG TCACCTGTGC TCTTTGTGCA AATTGTGATC 
AAGACTTATG TGCCGGATGA GCTCAGTGGC CTCGTCGATG CTCTCGCCGA CCCACGAGCA 
TTCCTCTCGG AGCAGAAAAA AAGGCAGGAA GCCCTGGCTC ACATGGGTGT TGACGATAGT 
GATATTCCTG ATGTGCCCAA CACCAGGAAT ATGGCTCTCC GACACGTGAA ACAACCTCCA 
AGGCAGAACG GATCGTCAGC TGATCTGCTG GCAAACAACG GCCAAACTGG GTCGGCTAGG 
GGTGATCAGA CCAGCTCGAT GGCTTCAAGT ACTATTCGGT CACCCAACGA ACAACCGCAG 
CCAGTGGCTG TGGATAAATT TAAGGTTGAC CCAATCGAAG TTGACGATTT ACGACGAGAC 
AAAGCTTTTG CAAAATTGCT CAAGAGATTT CAAAAAGAGT TGGATGATCT CAGGAAGAAA 
CATCAGAAGC AGAGGGATAG CATTCAAAAA CAGCAGCAAA CCAACGTCGA CAAGCTCATC 
ACAAATAACC GACGTTCCAC GAAAAAAGAA AAAGGCTCAA GGAGATCCCT GACGGCTTCA 
GTATCATCTG GATGTGGAAG TGCATCTGGA ACTGTGACTG TCAGTGTTTG CAGTCCTTCT 
GGCGCGAGTT GCAGTGGGTA CTCCACTGGG GGTCCTTCGA CACCGGTGGC ATGTAACTCT 
GATGGTACCG GGTCTCCAGC GACTATTGGA AGTCCAGTGC CACAAGATTT GGTCAATAAT 
GATAGGGTCC GATCTCTCGT GAACACTCAA ACCGGAGAAT GGTCCGCTAT GGTCCGTAGA 
CATGACGAGG AAGAGTTTGA GCTGAAAAAA GTACAGCTCA AAGAGCAATT CGATCTGCTA 
AGAAAACTCA TGAGTGAAGC TCAAAAGAAC CAAATGTTGG CTCTGAAGCT CCGGTTAGAA 
GCCGAAGGGA AAGATCTAAA GCAGACGCAG ACGAAAAAAA GCATGGAAGA TGCGAAAGTT 
ATTCAATTGG ACAAAGGTAT TAAGACCAAG GCAGAACGTG ATCGTCGTGT CAAGGAACTC 
AACGAGAAGA ACCTGAAAAT GTTTGTGGAG GAGAGGAAGA GATTAGCCAT GAAAGCCCAA 
AAGCACGAAG AGCAACTCAC AAAACGACAT CTCGACCAGC TCGAGCAACT AGACAAGGAT 
TTCCATAAGG CCTTGGATGC GGAGGTCGGC AACTACAAAG AAGAGCAACT GGCTGCTCAA 
CCGACTTCTG TCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313085.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300014.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

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CloneID	OCa348019
Clone ID Related Accession (Same CDS sequence)	NM_001313085.1 , NM_001313085.2
Accession Version	NM_001313085.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCCGGCTCGC CGCCGTAATT CGTCCATAGC GTGGATCCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300014.1
CDS	1..4539
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313085.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313085.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCCGGCTCGC CGCCGTAATT CGTCCATAGC GTGGATCCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313085.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300014.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

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CloneID	OCa348019
Clone ID Related Accession (Same CDS sequence)	NM_001313085.1 , NM_001313085.2
Accession Version	NM_001313085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001313085.1.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCCGGCTCGC CGCCGTAATT CGTCCATAGC GTGGATCCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300014.1
CDS	183..4721
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313085.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313085.2

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCCGGCTCGC CGCCGTAATT CGTCCATAGC GTGGATCCAA 
ACCAACGTCG ACAAGCTCAT CACAAATAAC CGACGTTCCA CGAAAAAAGA AAAAGGCTCA 
AGGAGATCCC TGACGGCTTC AGTATCATCT GGATGTGGAA GTGCATCTGG AACTGTGACT 
GTCAGTGTTT GCAGTCCTTC TGGCGCGAGT TGCAGTGGGT ACTCCACTGG GGGTCCTTCG 
ACACCGGTGG CATGTAACTC TGATGGTACC GGGTCTCCAG CGACTATTGG AAGTCCAGTG 
CCACAAGATT TGGTCAATAA TGATAGGGTC CGATCTCTCG TGAACACTCA AACCGGAGAA 
TGGTCCGCTA TGGTCCGTAG ACATGACGAG GAAGAGTTTG AGCTGAAAAA AGTACAGCTC 
AAAGAGCAAT TCGATCTGCT AAGAAAACTC ATGAGTGAAG CTCAAAAGAA CCAAATGTTG 
GCTCTGAAGC TCCGGTTAGA AGCCGAAGGG AAAGATCTAA AGCAGACGCA GACGAAAAAA 
AGCATGGAAG ATGCGAAAGT TATTCAATTG GACAAAGGTA TTAAGACCAA GGCAGAACGT 
GATCGTCGTG TCAAGGAACT CAACGAGAAG AACCTGAAAA TGTTTGTGGA GGAGAGGAAG 
AGATTAGCCA TGAAAGCCCA AAAGCACGAA GAGCAACTCA CAAAACGACA TCTCGACCAG 
CTCGAGCAAC TAGACAAGGA TTTCCATAAG GCCTTGGATG CGGAGGTCGG CAACTACAAA 
GAAGAGCAAC TGGCTGCTCA ACCGACTTCT GTCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001313086.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300015.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

Price & Availability↑

CloneID	OCa348020
Clone ID Related Accession (Same CDS sequence)	NM_001313086.1 , NM_001372978.1
Accession Version	NM_001313086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCAAACCAAC GTCGACAAGC TCATCACAAA TAACCGACGT 
TCCACGAAAA AAGAAAAAGG CTCAAGGAGA TCCCTGACGG CTTCAGTATC ATCTGGATGT 
GGAAGTGCAT CTGGAACTGT GACTGTCAGT GTTTGCAGTC CTTCTGGCGC GAGTTGCAGT 
GGGTACTCCA CTGGGGGTCC TTCGACACCG GTGGCATGTA ACTCTGATGG TACCGGGTCT 
CCAGCGACTA TTGGAAGTCC AGTGCCACAA GATTTGGTCA ATAATGATAG GGTCCGATCT 
CTCGTGAACA CTCAAACCGG AGAATGGTCC GCTATGGTCC GTAGACATGA CGAGGAAGAG 
TTTGAGCTGA AAAAAGTACA GCTCAAAGAG CAATTCGATC TGCTAAGAAA ACTCATGAGT 
GAAGCTCAAA AGAACCAAAT GTTGGCTCTG AAGCTCCGGT TAGAAGCCGA AGGGAAAGAT 
CTAAAGCAGA CGCAGACGAA AAAAAGCATG GAAGATGCGA AAGTTATTCA ATTGGACAAA 
GGTATTAAGA CCAAGGCAGA ACGTGATCGT CGTGTCAAGG AACTCAACGA GAAGAACCTG 
AAAATGTTTG TGGAGGAGAG GAAGAGATTA GCCATGAAAG CCCAAAAGCA CGAAGAGCAA 
CTCACAAAAC GACATCTCGA CCAGCTCGAG CAACTAGACA AGGATTTCCA TAAGGCCTTG 
GATGCGGAGG TCGGCAACTA CAAAGAAGAG CAACTGGCTG CTCAACCGAC TTCTGTCGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300015.1
CDS	1..4503
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001313086.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313086.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCAAACCAAC GTCGACAAGC TCATCACAAA TAACCGACGT 
TCCACGAAAA AAGAAAAAGG CTCAAGGAGA TCCCTGACGG CTTCAGTATC ATCTGGATGT 
GGAAGTGCAT CTGGAACTGT GACTGTCAGT GTTTGCAGTC CTTCTGGCGC GAGTTGCAGT 
GGGTACTCCA CTGGGGGTCC TTCGACACCG GTGGCATGTA ACTCTGATGG TACCGGGTCT 
CCAGCGACTA TTGGAAGTCC AGTGCCACAA GATTTGGTCA ATAATGATAG GGTCCGATCT 
CTCGTGAACA CTCAAACCGG AGAATGGTCC GCTATGGTCC GTAGACATGA CGAGGAAGAG 
TTTGAGCTGA AAAAAGTACA GCTCAAAGAG CAATTCGATC TGCTAAGAAA ACTCATGAGT 
GAAGCTCAAA AGAACCAAAT GTTGGCTCTG AAGCTCCGGT TAGAAGCCGA AGGGAAAGAT 
CTAAAGCAGA CGCAGACGAA AAAAAGCATG GAAGATGCGA AAGTTATTCA ATTGGACAAA 
GGTATTAAGA CCAAGGCAGA ACGTGATCGT CGTGTCAAGG AACTCAACGA GAAGAACCTG 
AAAATGTTTG TGGAGGAGAG GAAGAGATTA GCCATGAAAG CCCAAAAGCA CGAAGAGCAA 
CTCACAAAAC GACATCTCGA CCAGCTCGAG CAACTAGACA AGGATTTCCA TAAGGCCTTG 
GATGCGGAGG TCGGCAACTA CAAAGAAGAG CAACTGGCTG CTCAACCGAC TTCTGTCGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-8 ( NM_001372978.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360387.1 Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

Price & Availability↑

CloneID	OCa348020
Clone ID Related Accession (Same CDS sequence)	NM_001313086.1 , NM_001372978.1
Accession Version	NM_001372978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCAAACCAAC GTCGACAAGC TCATCACAAA TAACCGACGT 
TCCACGAAAA AAGAAAAAGG CTCAAGGAGA TCCCTGACGG CTTCAGTATC ATCTGGATGT 
GGAAGTGCAT CTGGAACTGT GACTGTCAGT GTTTGCAGTC CTTCTGGCGC GAGTTGCAGT 
GGGTACTCCA CTGGGGGTCC TTCGACACCG GTGGCATGTA ACTCTGATGG TACCGGGTCT 
CCAGCGACTA TTGGAAGTCC AGTGCCACAA GATTTGGTCA ATAATGATAG GGTCCGATCT 
CTCGTGAACA CTCAAACCGG AGAATGGTCC GCTATGGTCC GTAGACATGA CGAGGAAGAG 
TTTGAGCTGA AAAAAGTACA GCTCAAAGAG CAATTCGATC TGCTAAGAAA ACTCATGAGT 
GAAGCTCAAA AGAACCAAAT GTTGGCTCTG AAGCTCCGGT TAGAAGCCGA AGGGAAAGAT 
CTAAAGCAGA CGCAGACGAA AAAAAGCATG GAAGATGCGA AAGTTATTCA ATTGGACAAA 
GGTATTAAGA CCAAGGCAGA ACGTGATCGT CGTGTCAAGG AACTCAACGA GAAGAACCTG 
AAAATGTTTG TGGAGGAGAG GAAGAGATTA GCCATGAAAG CCCAAAAGCA CGAAGAGCAA 
CTCACAAAAC GACATCTCGA CCAGCTCGAG CAACTAGACA AGGATTTCCA TAAGGCCTTG 
GATGCGGAGG TCGGCAACTA CAAAGAAGAG CAACTGGCTG CTCAACCGAC TTCTGTCGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360387.1
CDS	183..4685
Translation	MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPDSSTSLLTASPTPSSSSMAMSNANNPFSSSTLNSPQPMERSRSEKRSFRQKKGGVFGDELHSDSAALIDFMRTASSRKKKPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV

Target ORF information:

RefSeq Version	NM_001372978.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (egl-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372978.1

ORF Insert Sequence:

ATGGCAAAGG AGTTCCAGTT CAATTGGAAG CCCACTATTA TCCCTGAACT TCTTCATGGA 
TCGGTTTTCG ATCGATATGA TGATGAAAGT ACGTGTCTGG AACTAAATGC TCAAGTTCGA 
ATTGACGAGA ACGGTTTCTT TCTCCGGTGG CTGATCGAAG GAAAAGATGC TGTCGTACTC 
GATATGGGCC AAATATGGGA AGCTCGCACG GGCGGCCTAC CCAAAGACGG CCGAATTATG 
TTCGAATTGG AGCAGCGCGG AGCCAGCGAA ACGATAGCTG AACGCACGAT ATGGATCACG 
CATGGCCAGG ATTTAGTCAA TGTTCAGTCG TTTTTTCTTG TCGCCGAAAG TGTCGAGTTG 
GCAAAAACGT GTAGAGCGGG CATTAATGAT ATTCTAAAGT CGTCCAGAAT ACGACACGTG 
TGTCCAACTA CCCAATTGAT GAAATACCAT ACATGGCTCA CCATGAATGT CAACGAACGA 
CGAAAAATCC CGATCAAGTT GATAATCAAA ACATTTTCGT CTGGAAAACC TGAAAAAATG 
GTTCAAAAGT GTCTAAATGA CTTGGGTCTT GGAGGCGATA AGTACACGCC GGCACGGGTC 
ATCAATCGAT CGATGGGCAA GAAGTTTCGG AATTTCTACA AGTGCAGTCG GGGACGAAAA 
CGAAAGGAGC GCGAAGAACT TGACGTCGAC ATCCTCACCT TTGAAAAATT CCAGAGGCTC 
TATAACAAGA TTTGTCCGAG AACCGAAGTT CAAGAGCTGT TTGTTAAATT ATCCGGACAG 
AAAGAGTACC TAACCAAAGA GCGACTCATC AATTTTCTGA ATGAAGAACA ACGTGATCCA 
CGACTCAACG AGATCCTCTT CCCATTTTTT GATTCTCAGC GTATCGTTGC GCTTCTCAAA 
AAACACGAAA ACGACATAAA ATATCAAGAA GATGGAAAAA TGTCCGGTGA CGGGTTTCTC 
CGATTCCTGA TGTCAGACGA GAACCCGCCA GTGTTTCTAG ACCGAATTGA AATGTTTATG 
GATATGGATC AACCGCTGTG TCACTACTAC ATCAATAGTT CCCATAACAC GTATCTTACC 
GGACGGCAGT ATGGTGGAAA ATCGTCTTCT GAGATTTATC GACAAGTTTT GCTCAGCGGA 
TGTAGGTGTA TTGAGCTTGA TTGTTGGGAT GGTACTGGTG AAAATAAAGG AGAACCCATT 
ATCACTCACG GAAAGGCCAT GTGCACAGAC GTGTTCTTCA AGGACGTCCT GGTACAAATT 
CGAGACACCG CATTTGCCCG GTCGGATTTT CCAGTGGTCC TTTCCTTCGA AAACCATTGC 
TCAAAATCAA ATCAGCTGAA AATGGCCAAA TATTGTATGG ACATTTTTGG AGATATGCTG 
CTGTCCAAAC CGTTTGAAGA TGCGCCTCTT GACCCCGGAG TCTCCCTACC TTCTCCAAAC 
CGCCTGCGAA AGAAGATCCT TATCAAAAAT AAGCGATTAA AAACCGACAT CGAACGTCAC 
CAACTTGACC AATTTCTGCG GGAAGGCAAA CTGGACGAGG AGGACGAGCT GAATGAGACG 
CCCGAAGTTG TCGGAGAGGA CTCCGTATCA CCTCGAAGCG GCGGGAGCGG TGGCACCGGA 
GCCCCGGAAG AAGTGGATGA TGACACGTCC GACGACGACG ATGACCCGTC GGTTCAGACA 
TCGCTGAACG TGATGCGCAC CATCCCGACC GTGAACACCA CATCGAACAA CGGATCGAAT 
CGATCGGCAA GAAGTTCACT GGACACCCCA TCCCCATCCG GTGGGAGCCT GATGGTTCCA 
GATAGGGCAA CATCGACAGC GACGTCGATC AAGAACGCCG TGCTCGCGAG GTCTCCGAAC 
TTCTCCTCGT TGAGACAAAA GTTGTCGTTC AAGCGGAGAC AATCACCACT AGCAGGCGAT 
CAGCGTGCTC ATCCGGAAGT CGAGCAACCC GTGAGCTCAT CCTCTCCAGC CACACCGTCC 
ATCAGCGGAC CCCCGCCGTG TGCCACGTCA TCAGGTTCAA CGTCATCAAT CACTATTACC 
ACAACGGGAT GCTCCACGTC ATCGTCGGGA CCCTCAAAAC ATATTCTAGG GGGTGAAATG 
CCCGCCAAGG AAAACGACGA AGCACATCCG GAGCTAAAGC AGAATTTCAT TGCTAAAAAT 
TTGAAAGGAT TCGGGTTTTC GAAAAAACAA CCTGATTCAT CCACTTCATT GCTAACCGCA 
TCACCAACAC CGTCCTCTTC ATCGATGGCC ATGTCAAATG CGAATAATCC GTTCTCCTCC 
TCAACCCTTA ACTCACCCCA ACCAATGGAG AGGTCTCGAA GCGAAAAGCG ATCGTTTCGA 
CAGAAAAAGG GCGGCGTTTT CGGTGACGAA CTCCACTCGG ACTCGGCCGC ACTTATCGAT 
TTTATGCGGA CGGCGTCGAG CCGTAAGAAG AAGCCGGTAC TGACAAAAGA AGAAGAGGAG 
CGGATTTTCG CCGAGTATCA TTATACCGGG GCAACTACGA ATATACATCC GTTACTCTCT 
TCACTGGTCA ATTATACACA TCCTGTCAAG TTTTCTGGAT TTGACGTTGC GGAAGCCAAC 
AACCTGCACT TCCACATGTC CTCATTTTCT GAGTCAACCG GTCTCGGCTA CCTAAAACAA 
TCGGCGCCGG AATTTGTGAA CTACAACAAA CGACAATCGA GTAGAATTTA TCCGAAAGGA 
GCCAGAGTTG ATTCTTCCAA TTTTTTGCCG CAAATTTTCT GGAACGCCGG ATGTCAAATG 
GTGTCGCTCA ACTTTCAAAC TCCCGATGTC TACATGCAAT TGAACATGGG AAAATTCGAG 
TACAACGGTG GCTCGGGGTA TCTGCTAAAA CCAGATTTCC TCCGAAGACC CGACAGAACA 
TTCGACCCAT TTTCCGAGAG CCCGGTGGAC GGAGTGATTG CAGCACATTG TAGTGTTCGT 
GTCATCTCGG GTCAATTCCT ATCGGATCGC AAAATCGGGA CATACGTTGA GGTGGAAATG 
TACGGACTAC CTACTGACAC TATTCGTAAG GAGCACAAGA CGAAGGTAAT ACCCGGGAAC 
GGACTGAATC CAGTTTACAA CGAGGATCCG TTTGTCTTCC GAAAAGTTGT GCTCCCCGAG 
CTCGCCGTGC TCCGGTTCGC CGTCTACGAT GAGAACGGCA AGCAGCTGGG TCAGCGGATT 
CTTCCGCTGG ATGGGCTTCA GGCAGGATAT CGGCATATTT CGCTGAGGTC TGACACGAAT 
CAGAGCTTCA TTCTGTCACC TGTGCTCTTT GTGCAAATTG TGATCAAGAC TTATGTGCCG 
GATGAGCTCA GTGGCCTCGT CGATGCTCTC GCCGACCCAC GAGCATTCCT CTCGGAGCAG 
AAAAAAAGGC AGGAAGCCCT GGCTCACATG GGTGTTGACG ATAGTGATAT TCCTGATGTG 
CCCAACACCA GGAATATGGC TCTCCGACAC GTGAAACAAC CTCCAAGGCA GAACGGATCG 
TCAGCTGATC TGCTGGCAAA CAACGGCCAA ACTGGGTCGG CTAGGGGTGA TCAGACCAGC 
TCGATGGCTT CAAGTACTAT TCGGTCACCC AACGAACAAC CGCAGCCAGT GGCTGTGGAT 
AAATTTAAGG TTGACCCAAT CGAAGTTGAC GATTTACGAC GAGACAAAGC TTTTGCAAAA 
TTGCTCAAGA GATTTCAAAA AGAGTTGGAT GATCTCAGGA AGAAACATCA GAAGCAGAGG 
GATAGCATTC AAAAACAGCA GCAAACCAAC GTCGACAAGC TCATCACAAA TAACCGACGT 
TCCACGAAAA AAGAAAAAGG CTCAAGGAGA TCCCTGACGG CTTCAGTATC ATCTGGATGT 
GGAAGTGCAT CTGGAACTGT GACTGTCAGT GTTTGCAGTC CTTCTGGCGC GAGTTGCAGT 
GGGTACTCCA CTGGGGGTCC TTCGACACCG GTGGCATGTA ACTCTGATGG TACCGGGTCT 
CCAGCGACTA TTGGAAGTCC AGTGCCACAA GATTTGGTCA ATAATGATAG GGTCCGATCT 
CTCGTGAACA CTCAAACCGG AGAATGGTCC GCTATGGTCC GTAGACATGA CGAGGAAGAG 
TTTGAGCTGA AAAAAGTACA GCTCAAAGAG CAATTCGATC TGCTAAGAAA ACTCATGAGT 
GAAGCTCAAA AGAACCAAAT GTTGGCTCTG AAGCTCCGGT TAGAAGCCGA AGGGAAAGAT 
CTAAAGCAGA CGCAGACGAA AAAAAGCATG GAAGATGCGA AAGTTATTCA ATTGGACAAA 
GGTATTAAGA CCAAGGCAGA ACGTGATCGT CGTGTCAAGG AACTCAACGA GAAGAACCTG 
AAAATGTTTG TGGAGGAGAG GAAGAGATTA GCCATGAAAG CCCAAAAGCA CGAAGAGCAA 
CTCACAAAAC GACATCTCGA CCAGCTCGAG CAACTAGACA AGGATTTCCA TAAGGCCTTG 
GATGCGGAGG TCGGCAACTA CAAAGAAGAG CAACTGGCTG CTCAACCGAC TTCTGTCGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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オンライン注文

egl-8 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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Target ORF information:

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