ddb-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ddb-1
Entrez Gene ID	178156
Full Name	DNA damage-binding protein 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_069898.7	NP_502299.1	DNA damage-binding protein 1
NM_069898.8	NP_502299.1	DNA damage-binding protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that SKN-1 protein levels, nuclear accumulation, and activity are repressed by the WD40 repeat protein WDR-23, which interacts with the CUL-4/DDB-1 ubiquitin ligase to presumably target the transcription factor for proteasomal degradation.
Title: The WD40 repeat protein WDR-23 functions with the CUL4/DDB1 ubiquitin ligase to regulate nuclear abundance and activity of SKN-1 in Caenorhabditis elegans.

Caenorhabditis elegans DDB-1 is required for the degradation of CDT-1 during S phase. DDB-1 interacts specifically with CUL-4 but not with other C. elegans cullins.
Title: The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex.

The following ddb-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddb-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342348	NM_069898.8 Latest version!	Caenorhabditis elegans DNA damage-binding protein 1 (ddb-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa342348	NM_069898.7	Caenorhabditis elegans DNA damage-binding protein 1 (ddb-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddb-1 ( NM_069898.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502299.1 Caenorhabditis elegans DNA damage-binding protein 1 (ddb-1), partial mRNA.

Price & Availability↑

CloneID	OCa342348
Clone ID Related Accession (Same CDS sequence)	NM_069898.7 , NM_069898.8
Accession Version	NM_069898.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA damage-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069898.6. COMPLETENESS: incomplete on both ends.

ATGCCGATCA GTTACTGTGT TTCGGCAAAG AAGGCATCCG TTGTCGTAGA ATCTGTTGTC 
GGAAACTTCA CAGGACACGA AAATGTTAAC TTGATTGTGG CTCGTGGAAA TCGCATTGAT 
GTTCAACTAG TTTCACCGGA AGGACTCAAA AATGTCTGCG AGATCCCAAT CTACGGCCAA 
GTGTTGACAA TTGCACTTGT AAAATGTAAA CGCGATAAGC GTCATTCATT GATTGTTGTC 
ACCGAAAAAT GGCACATGGC AATTTTGGCG TATCGCGACG GAAAAGTTGT GACTCGTGCT 
GCTGGATGTA TTGCTGATCC GACTGGAAGA GCGACTGACA ATTTATTCAG TCTAACGATT 
CATCGAAACG GACTCATCGC TATTCGAGCT TTCGAGGGTA GTGTCAAAAT GATTCAATGG 
GAATCTGGTA CTGATCTACG TCATTTCAAT GTTAGATTTG ATTATCCAAA TGTCTCAGAC 
TTCAAGTTTG TTGACACTGG AGAAGATGAT GTATACCGAG TTGCCTTCAT CTATGACGAC 
GATCACGGAA AACATCTTCA ATTTTCCGAT CTTAACATGC ATGACAAGGA ATTCCGTACC 
TATTCCAGAC AAGCCAGTAT TGCTGCTGAT TCTTCGGTTT TAATTCCAGT TCCTCATGCT 
ATTGGAGGTG TTATAGTTCT CGGATCGAAC TCTGTCCTAT ACAAGCCAAA CGACAACCTC 
GGAGAAGTAG TTCCATACAC TTGCTCTTTG CTCGAGAACA CAACATTCAC ATGCCACGGA 
ATCGTTGATG CTTCTGGTGA AAGATTTCTT CTTTCCGATA CAGATGGACG TCTTCTAATG 
CTACTGTTGA ATGTAACTGA AAGTCAATCA GGATACACAG TTAAAGAAAT GCGTATCGAT 
TATCTTGGAG AAACTTCAAT TGCTGATTCC ATCAATTATA TTGACAATGG AGTAGTCTTT 
GTTGGTTCTC GACTCGGAGA TTCACAGCTT ATCAGGCTTA TGACTGAACC GAATGGAGGA 
AGTTATTCTG TGATACTTGA AACCTACAGT AACATTGGAC CAATTCGTGA TATGGTTATG 
GTAGAGTCTG ATGGACAACC ACAACTTGTA ACCTGTACTG GAGCTGACAA AGACGGATCA 
CTTCGTGTTA TTCGTAATGG AATCGGCATC GATGAATTGG CTAGTGTTGA TTTGGCTGGA 
GTCGTCGGCA TATTTCCCAT TCGTCTAGAT TCGAATGCTG ACAACTATGT AATTGTTTCA 
CTTTCTGATG AAACCCACGT TCTGCAAATT ACGGGAGAAG AGCTTGAAGA TGTGAAACTT 
TTGGAAATCA ACACAGATTT GCCAACAATT TTCGCGTCCA CTTTGTTTGG TCCAAACGAT 
TCTGGAATCA TTCTTCAAGC AACGGAAAAG CAGATTCGAC TTATGTCTTC TTCGGGACTT 
TCCAAATTTT GGGAACCAAC AAACGGTGAA ATTATTTCGA AAGTTTCTGT GAATGCAGCC 
AACGGACAAA TTGTTCTCGC AGCTAGAGAT ACAGTATACC TTCTCACTTG TATTGTTGAT 
GAAATGGGTG CTCTCGACAT TCAATTGACT GCTGAAAAGA AGTTTGAAAA CGAAATTGCA 
TGTTTGGACC TTTCGAATGA AGGAGACGAT CCTAACAATA AAGCAACCTT CTTGGTTTTA 
GCATTTTGGA GTACATTTGC GATGGAAGTA ATTCAGCTAC CTGATCTTAT AACTGTGTGC 
CACACGGATC TTCCAACAAA GATCATTCCT CGTTCGATCA TTGCAACATG CATAGAAGAA 
GTTCATTATC TTCTTGTTGC TTTCGGAGAC GGTGCACTTG TTTATTATGT GTTTGATATC 
AAAACAGGAA CACACGGTGA ACCAAAAAAG TCAAATGTCG GAACACGACC ACCGAGTCTT 
CATCGTGTAC GCAACAAGAA TCGACAACAC TTGTTCGTCT GTTCTGATCG TCCAGTGATC 
ATCTTCTCGG CCAGCAAAAA GCTTGTCTTC TCCAATGTTA ATGTTAAACT CGTCGATACT 
GTGTGCTCGC TGAGTTCCAG CGCCTATCGA GATTGTTTGG TCATTTCGGA TGGAAATTCG 
ATGGTTTTTG GCACAGTTGA CGATATTCAG AAGATCCACG TTCGCTCGAT TCCAATGGGA 
GAATCTGTTC TTCGTATTGC ATATCAAAAG AGCACAAGTA CATATGGAGT CTGCTCGAAT 
CGAACTGAAT CGAAGGCTGA ACGAGTTTTT GCTTCGAAAA ATGCACTTGT CACTTCTCAA 
AGTCGACCAA AAGTTGCATC CACCCGAGCT GACATGGATG AAAGTCCTCC AAATACCACA 
AGCAGTTTTA TGGTTCTCGA TCAAAACACA TTTCAAGTAC TTCATTCACA TGAATTCGGA 
CCATGGGAAA CTGCGTTGAG TTGCATCTCT GGACAATTCA CAAACGACTC GAGCACCTAC 
TACGTTGTTG GAACTGGTCT TATCTATCCA GACGAAACAG AGACGAAGAT CGGTAGAATC 
GTGGTATTTG AAGTTGACGA CGTGGAAAGA TCGAAACTTC GACGTGTACA TGAATTGGTT 
GTTCGTGGAA GTCCTTTAGC TATTCGAATT CTGAATGGAA AATTGGTTGC TGCAATTAAT 
AGCTCAATTA GACTATTCGA ATGGACAACT GATAAGGAAC TTCGACTGGA ATGCAGTAGC 
TTCAATCATG TCATTGCACT TGATCTTAAA GTTATGAATG AAGAGGTTGC AGTGGCTGAT 
GTGATGAGAT CTGTCTCTCT TCTCTCGTAC AGAATGCTGG AAGGAAATTT TGAGGAAGTT 
GCTAAAGATT GGAATAGTCA ATGGATGGTT ACTTGCGAGT TCATCACAGC TGAATCAATC 
CTTGGAGGAG AAGCTCATCT GAATCTGTTC ACTGTAGAAG TTGATAAAAC CCGTCCGATT 
ACTGATGATG GTCGATATGT GTTGGAACCA ACTGGATATT GGTATCTCGG AGAGCTTCCA 
AAAGTCATGA CAAGATCGAC TCTTGTCATT CAACCAGAAG ATAGTATTAT CCAGTACAGT 
CAACCAATCA TGTTTGGAAC AAATCAGGGA ACAATTGGAA TGATTGTGCA GATTGATGAC 
AAATGGAAAA AGTTTTTGAT TGCAATTGAA AAGGCTATCG CAGATTCCGT TAAAAACTGC 
ATGCACATTG AACATTCCAG TTACAGAACT TTTGTGTTCC AGAAAAGAGC GGAACCGCCA 
TCCGGATTCG TCGATGGAGA TCTCGTTGAA TCGATTCTCG ACATGGATCG TTCTGTTGCA 
ATGGATATCT TGAGTAAAGT ATCTGACAAA GGATGGGATC CAAGTCTGCC AAGAGATCCA 
GTTGAAATTC TCAAAGTCAT CGAGGATTTG GCGAGAATGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502299.1
CDS	1..3405
Translation	MPISYCVSAKKASVVVESVVGNFTGHENVNLIVARGNRIDVQLVSPEGLKNVCEIPIYGQVLTIALVKCKRDKRHSLIVVTEKWHMAILAYRDGKVVTRAAGCIADPTGRATDNLFSLTIHRNGLIAIRAFEGSVKMIQWESGTDLRHFNVRFDYPNVSDFKFVDTGEDDVYRVAFIYDDDHGKHLQFSDLNMHDKEFRTYSRQASIAADSSVLIPVPHAIGGVIVLGSNSVLYKPNDNLGEVVPYTCSLLENTTFTCHGIVDASGERFLLSDTDGRLLMLLLNVTESQSGYTVKEMRIDYLGETSIADSINYIDNGVVFVGSRLGDSQLIRLMTEPNGGSYSVILETYSNIGPIRDMVMVESDGQPQLVTCTGADKDGSLRVIRNGIGIDELASVDLAGVVGIFPIRLDSNADNYVIVSLSDETHVLQITGEELEDVKLLEINTDLPTIFASTLFGPNDSGIILQATEKQIRLMSSSGLSKFWEPTNGEIISKVSVNAANGQIVLAARDTVYLLTCIVDEMGALDIQLTAEKKFENEIACLDLSNEGDDPNNKATFLVLAFWSTFAMEVIQLPDLITVCHTDLPTKIIPRSIIATCIEEVHYLLVAFGDGALVYYVFDIKTGTHGEPKKSNVGTRPPSLHRVRNKNRQHLFVCSDRPVIIFSASKKLVFSNVNVKLVDTVCSLSSSAYRDCLVISDGNSMVFGTVDDIQKIHVRSIPMGESVLRIAYQKSTSTYGVCSNRTESKAERVFASKNALVTSQSRPKVASTRADMDESPPNTTSSFMVLDQNTFQVLHSHEFGPWETALSCISGQFTNDSSTYYVVGTGLIYPDETETKIGRIVVFEVDDVERSKLRRVHELVVRGSPLAIRILNGKLVAAINSSIRLFEWTTDKELRLECSSFNHVIALDLKVMNEEVAVADVMRSVSLLSYRMLEGNFEEVAKDWNSQWMVTCEFITAESILGGEAHLNLFTVEVDKTRPITDDGRYVLEPTGYWYLGELPKVMTRSTLVIQPEDSIIQYSQPIMFGTNQGTIGMIVQIDDKWKKFLIAIEKAIADSVKNCMHIEHSSYRTFVFQKRAEPPSGFVDGDLVESILDMDRSVAMDILSKVSDKGWDPSLPRDPVEILKVIEDLARMH

Target ORF information:

RefSeq Version	NM_069898.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA damage-binding protein 1 (ddb-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069898.7

ORF Insert Sequence:

ATGCCGATCA GTTACTGTGT TTCGGCAAAG AAGGCATCCG TTGTCGTAGA ATCTGTTGTC 
GGAAACTTCA CAGGACACGA AAATGTTAAC TTGATTGTGG CTCGTGGAAA TCGCATTGAT 
GTTCAACTAG TTTCACCGGA AGGACTCAAA AATGTCTGCG AGATCCCAAT CTACGGCCAA 
GTGTTGACAA TTGCACTTGT AAAATGTAAA CGCGATAAGC GTCATTCATT GATTGTTGTC 
ACCGAAAAAT GGCACATGGC AATTTTGGCG TATCGCGACG GAAAAGTTGT GACTCGTGCT 
GCTGGATGTA TTGCTGATCC GACTGGAAGA GCGACTGACA ATTTATTCAG TCTAACGATT 
CATCGAAACG GACTCATCGC TATTCGAGCT TTCGAGGGTA GTGTCAAAAT GATTCAATGG 
GAATCTGGTA CTGATCTACG TCATTTCAAT GTTAGATTTG ATTATCCAAA TGTCTCAGAC 
TTCAAGTTTG TTGACACTGG AGAAGATGAT GTATACCGAG TTGCCTTCAT CTATGACGAC 
GATCACGGAA AACATCTTCA ATTTTCCGAT CTTAACATGC ATGACAAGGA ATTCCGTACC 
TATTCCAGAC AAGCCAGTAT TGCTGCTGAT TCTTCGGTTT TAATTCCAGT TCCTCATGCT 
ATTGGAGGTG TTATAGTTCT CGGATCGAAC TCTGTCCTAT ACAAGCCAAA CGACAACCTC 
GGAGAAGTAG TTCCATACAC TTGCTCTTTG CTCGAGAACA CAACATTCAC ATGCCACGGA 
ATCGTTGATG CTTCTGGTGA AAGATTTCTT CTTTCCGATA CAGATGGACG TCTTCTAATG 
CTACTGTTGA ATGTAACTGA AAGTCAATCA GGATACACAG TTAAAGAAAT GCGTATCGAT 
TATCTTGGAG AAACTTCAAT TGCTGATTCC ATCAATTATA TTGACAATGG AGTAGTCTTT 
GTTGGTTCTC GACTCGGAGA TTCACAGCTT ATCAGGCTTA TGACTGAACC GAATGGAGGA 
AGTTATTCTG TGATACTTGA AACCTACAGT AACATTGGAC CAATTCGTGA TATGGTTATG 
GTAGAGTCTG ATGGACAACC ACAACTTGTA ACCTGTACTG GAGCTGACAA AGACGGATCA 
CTTCGTGTTA TTCGTAATGG AATCGGCATC GATGAATTGG CTAGTGTTGA TTTGGCTGGA 
GTCGTCGGCA TATTTCCCAT TCGTCTAGAT TCGAATGCTG ACAACTATGT AATTGTTTCA 
CTTTCTGATG AAACCCACGT TCTGCAAATT ACGGGAGAAG AGCTTGAAGA TGTGAAACTT 
TTGGAAATCA ACACAGATTT GCCAACAATT TTCGCGTCCA CTTTGTTTGG TCCAAACGAT 
TCTGGAATCA TTCTTCAAGC AACGGAAAAG CAGATTCGAC TTATGTCTTC TTCGGGACTT 
TCCAAATTTT GGGAACCAAC AAACGGTGAA ATTATTTCGA AAGTTTCTGT GAATGCAGCC 
AACGGACAAA TTGTTCTCGC AGCTAGAGAT ACAGTATACC TTCTCACTTG TATTGTTGAT 
GAAATGGGTG CTCTCGACAT TCAATTGACT GCTGAAAAGA AGTTTGAAAA CGAAATTGCA 
TGTTTGGACC TTTCGAATGA AGGAGACGAT CCTAACAATA AAGCAACCTT CTTGGTTTTA 
GCATTTTGGA GTACATTTGC GATGGAAGTA ATTCAGCTAC CTGATCTTAT AACTGTGTGC 
CACACGGATC TTCCAACAAA GATCATTCCT CGTTCGATCA TTGCAACATG CATAGAAGAA 
GTTCATTATC TTCTTGTTGC TTTCGGAGAC GGTGCACTTG TTTATTATGT GTTTGATATC 
AAAACAGGAA CACACGGTGA ACCAAAAAAG TCAAATGTCG GAACACGACC ACCGAGTCTT 
CATCGTGTAC GCAACAAGAA TCGACAACAC TTGTTCGTCT GTTCTGATCG TCCAGTGATC 
ATCTTCTCGG CCAGCAAAAA GCTTGTCTTC TCCAATGTTA ATGTTAAACT CGTCGATACT 
GTGTGCTCGC TGAGTTCCAG CGCCTATCGA GATTGTTTGG TCATTTCGGA TGGAAATTCG 
ATGGTTTTTG GCACAGTTGA CGATATTCAG AAGATCCACG TTCGCTCGAT TCCAATGGGA 
GAATCTGTTC TTCGTATTGC ATATCAAAAG AGCACAAGTA CATATGGAGT CTGCTCGAAT 
CGAACTGAAT CGAAGGCTGA ACGAGTTTTT GCTTCGAAAA ATGCACTTGT CACTTCTCAA 
AGTCGACCAA AAGTTGCATC CACCCGAGCT GACATGGATG AAAGTCCTCC AAATACCACA 
AGCAGTTTTA TGGTTCTCGA TCAAAACACA TTTCAAGTAC TTCATTCACA TGAATTCGGA 
CCATGGGAAA CTGCGTTGAG TTGCATCTCT GGACAATTCA CAAACGACTC GAGCACCTAC 
TACGTTGTTG GAACTGGTCT TATCTATCCA GACGAAACAG AGACGAAGAT CGGTAGAATC 
GTGGTATTTG AAGTTGACGA CGTGGAAAGA TCGAAACTTC GACGTGTACA TGAATTGGTT 
GTTCGTGGAA GTCCTTTAGC TATTCGAATT CTGAATGGAA AATTGGTTGC TGCAATTAAT 
AGCTCAATTA GACTATTCGA ATGGACAACT GATAAGGAAC TTCGACTGGA ATGCAGTAGC 
TTCAATCATG TCATTGCACT TGATCTTAAA GTTATGAATG AAGAGGTTGC AGTGGCTGAT 
GTGATGAGAT CTGTCTCTCT TCTCTCGTAC AGAATGCTGG AAGGAAATTT TGAGGAAGTT 
GCTAAAGATT GGAATAGTCA ATGGATGGTT ACTTGCGAGT TCATCACAGC TGAATCAATC 
CTTGGAGGAG AAGCTCATCT GAATCTGTTC ACTGTAGAAG TTGATAAAAC CCGTCCGATT 
ACTGATGATG GTCGATATGT GTTGGAACCA ACTGGATATT GGTATCTCGG AGAGCTTCCA 
AAAGTCATGA CAAGATCGAC TCTTGTCATT CAACCAGAAG ATAGTATTAT CCAGTACAGT 
CAACCAATCA TGTTTGGAAC AAATCAGGGA ACAATTGGAA TGATTGTGCA GATTGATGAC 
AAATGGAAAA AGTTTTTGAT TGCAATTGAA AAGGCTATCG CAGATTCCGT TAAAAACTGC 
ATGCACATTG AACATTCCAG TTACAGAACT TTTGTGTTCC AGAAAAGAGC GGAACCGCCA 
TCCGGATTCG TCGATGGAGA TCTCGTTGAA TCGATTCTCG ACATGGATCG TTCTGTTGCA 
ATGGATATCT TGAGTAAAGT ATCTGACAAA GGATGGGATC CAAGTCTGCC AAGAGATCCA 
GTTGAAATTC TCAAAGTCAT CGAGGATTTG GCGAGAATGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddb-1 ( NM_069898.8 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502299.1 Caenorhabditis elegans DNA damage-binding protein 1 (ddb-1), mRNA.

Price & Availability↑

CloneID	OCa342348
Clone ID Related Accession (Same CDS sequence)	NM_069898.7 , NM_069898.8
Accession Version	NM_069898.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA damage-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069898.7.

ATGCCGATCA GTTACTGTGT TTCGGCAAAG AAGGCATCCG TTGTCGTAGA ATCTGTTGTC 
GGAAACTTCA CAGGACACGA AAATGTTAAC TTGATTGTGG CTCGTGGAAA TCGCATTGAT 
GTTCAACTAG TTTCACCGGA AGGACTCAAA AATGTCTGCG AGATCCCAAT CTACGGCCAA 
GTGTTGACAA TTGCACTTGT AAAATGTAAA CGCGATAAGC GTCATTCATT GATTGTTGTC 
ACCGAAAAAT GGCACATGGC AATTTTGGCG TATCGCGACG GAAAAGTTGT GACTCGTGCT 
GCTGGATGTA TTGCTGATCC GACTGGAAGA GCGACTGACA ATTTATTCAG TCTAACGATT 
CATCGAAACG GACTCATCGC TATTCGAGCT TTCGAGGGTA GTGTCAAAAT GATTCAATGG 
GAATCTGGTA CTGATCTACG TCATTTCAAT GTTAGATTTG ATTATCCAAA TGTCTCAGAC 
TTCAAGTTTG TTGACACTGG AGAAGATGAT GTATACCGAG TTGCCTTCAT CTATGACGAC 
GATCACGGAA AACATCTTCA ATTTTCCGAT CTTAACATGC ATGACAAGGA ATTCCGTACC 
TATTCCAGAC AAGCCAGTAT TGCTGCTGAT TCTTCGGTTT TAATTCCAGT TCCTCATGCT 
ATTGGAGGTG TTATAGTTCT CGGATCGAAC TCTGTCCTAT ACAAGCCAAA CGACAACCTC 
GGAGAAGTAG TTCCATACAC TTGCTCTTTG CTCGAGAACA CAACATTCAC ATGCCACGGA 
ATCGTTGATG CTTCTGGTGA AAGATTTCTT CTTTCCGATA CAGATGGACG TCTTCTAATG 
CTACTGTTGA ATGTAACTGA AAGTCAATCA GGATACACAG TTAAAGAAAT GCGTATCGAT 
TATCTTGGAG AAACTTCAAT TGCTGATTCC ATCAATTATA TTGACAATGG AGTAGTCTTT 
GTTGGTTCTC GACTCGGAGA TTCACAGCTT ATCAGGCTTA TGACTGAACC GAATGGAGGA 
AGTTATTCTG TGATACTTGA AACCTACAGT AACATTGGAC CAATTCGTGA TATGGTTATG 
GTAGAGTCTG ATGGACAACC ACAACTTGTA ACCTGTACTG GAGCTGACAA AGACGGATCA 
CTTCGTGTTA TTCGTAATGG AATCGGCATC GATGAATTGG CTAGTGTTGA TTTGGCTGGA 
GTCGTCGGCA TATTTCCCAT TCGTCTAGAT TCGAATGCTG ACAACTATGT AATTGTTTCA 
CTTTCTGATG AAACCCACGT TCTGCAAATT ACGGGAGAAG AGCTTGAAGA TGTGAAACTT 
TTGGAAATCA ACACAGATTT GCCAACAATT TTCGCGTCCA CTTTGTTTGG TCCAAACGAT 
TCTGGAATCA TTCTTCAAGC AACGGAAAAG CAGATTCGAC TTATGTCTTC TTCGGGACTT 
TCCAAATTTT GGGAACCAAC AAACGGTGAA ATTATTTCGA AAGTTTCTGT GAATGCAGCC 
AACGGACAAA TTGTTCTCGC AGCTAGAGAT ACAGTATACC TTCTCACTTG TATTGTTGAT 
GAAATGGGTG CTCTCGACAT TCAATTGACT GCTGAAAAGA AGTTTGAAAA CGAAATTGCA 
TGTTTGGACC TTTCGAATGA AGGAGACGAT CCTAACAATA AAGCAACCTT CTTGGTTTTA 
GCATTTTGGA GTACATTTGC GATGGAAGTA ATTCAGCTAC CTGATCTTAT AACTGTGTGC 
CACACGGATC TTCCAACAAA GATCATTCCT CGTTCGATCA TTGCAACATG CATAGAAGAA 
GTTCATTATC TTCTTGTTGC TTTCGGAGAC GGTGCACTTG TTTATTATGT GTTTGATATC 
AAAACAGGAA CACACGGTGA ACCAAAAAAG TCAAATGTCG GAACACGACC ACCGAGTCTT 
CATCGTGTAC GCAACAAGAA TCGACAACAC TTGTTCGTCT GTTCTGATCG TCCAGTGATC 
ATCTTCTCGG CCAGCAAAAA GCTTGTCTTC TCCAATGTTA ATGTTAAACT CGTCGATACT 
GTGTGCTCGC TGAGTTCCAG CGCCTATCGA GATTGTTTGG TCATTTCGGA TGGAAATTCG 
ATGGTTTTTG GCACAGTTGA CGATATTCAG AAGATCCACG TTCGCTCGAT TCCAATGGGA 
GAATCTGTTC TTCGTATTGC ATATCAAAAG AGCACAAGTA CATATGGAGT CTGCTCGAAT 
CGAACTGAAT CGAAGGCTGA ACGAGTTTTT GCTTCGAAAA ATGCACTTGT CACTTCTCAA 
AGTCGACCAA AAGTTGCATC CACCCGAGCT GACATGGATG AAAGTCCTCC AAATACCACA 
AGCAGTTTTA TGGTTCTCGA TCAAAACACA TTTCAAGTAC TTCATTCACA TGAATTCGGA 
CCATGGGAAA CTGCGTTGAG TTGCATCTCT GGACAATTCA CAAACGACTC GAGCACCTAC 
TACGTTGTTG GAACTGGTCT TATCTATCCA GACGAAACAG AGACGAAGAT CGGTAGAATC 
GTGGTATTTG AAGTTGACGA CGTGGAAAGA TCGAAACTTC GACGTGTACA TGAATTGGTT 
GTTCGTGGAA GTCCTTTAGC TATTCGAATT CTGAATGGAA AATTGGTTGC TGCAATTAAT 
AGCTCAATTA GACTATTCGA ATGGACAACT GATAAGGAAC TTCGACTGGA ATGCAGTAGC 
TTCAATCATG TCATTGCACT TGATCTTAAA GTTATGAATG AAGAGGTTGC AGTGGCTGAT 
GTGATGAGAT CTGTCTCTCT TCTCTCGTAC AGAATGCTGG AAGGAAATTT TGAGGAAGTT 
GCTAAAGATT GGAATAGTCA ATGGATGGTT ACTTGCGAGT TCATCACAGC TGAATCAATC 
CTTGGAGGAG AAGCTCATCT GAATCTGTTC ACTGTAGAAG TTGATAAAAC CCGTCCGATT 
ACTGATGATG GTCGATATGT GTTGGAACCA ACTGGATATT GGTATCTCGG AGAGCTTCCA 
AAAGTCATGA CAAGATCGAC TCTTGTCATT CAACCAGAAG ATAGTATTAT CCAGTACAGT 
CAACCAATCA TGTTTGGAAC AAATCAGGGA ACAATTGGAA TGATTGTGCA GATTGATGAC 
AAATGGAAAA AGTTTTTGAT TGCAATTGAA AAGGCTATCG CAGATTCCGT TAAAAACTGC 
ATGCACATTG AACATTCCAG TTACAGAACT TTTGTGTTCC AGAAAAGAGC GGAACCGCCA 
TCCGGATTCG TCGATGGAGA TCTCGTTGAA TCGATTCTCG ACATGGATCG TTCTGTTGCA 
ATGGATATCT TGAGTAAAGT ATCTGACAAA GGATGGGATC CAAGTCTGCC AAGAGATCCA 
GTTGAAATTC TCAAAGTCAT CGAGGATTTG GCGAGAATGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502299.1
CDS	69..3473
Translation	MPISYCVSAKKASVVVESVVGNFTGHENVNLIVARGNRIDVQLVSPEGLKNVCEIPIYGQVLTIALVKCKRDKRHSLIVVTEKWHMAILAYRDGKVVTRAAGCIADPTGRATDNLFSLTIHRNGLIAIRAFEGSVKMIQWESGTDLRHFNVRFDYPNVSDFKFVDTGEDDVYRVAFIYDDDHGKHLQFSDLNMHDKEFRTYSRQASIAADSSVLIPVPHAIGGVIVLGSNSVLYKPNDNLGEVVPYTCSLLENTTFTCHGIVDASGERFLLSDTDGRLLMLLLNVTESQSGYTVKEMRIDYLGETSIADSINYIDNGVVFVGSRLGDSQLIRLMTEPNGGSYSVILETYSNIGPIRDMVMVESDGQPQLVTCTGADKDGSLRVIRNGIGIDELASVDLAGVVGIFPIRLDSNADNYVIVSLSDETHVLQITGEELEDVKLLEINTDLPTIFASTLFGPNDSGIILQATEKQIRLMSSSGLSKFWEPTNGEIISKVSVNAANGQIVLAARDTVYLLTCIVDEMGALDIQLTAEKKFENEIACLDLSNEGDDPNNKATFLVLAFWSTFAMEVIQLPDLITVCHTDLPTKIIPRSIIATCIEEVHYLLVAFGDGALVYYVFDIKTGTHGEPKKSNVGTRPPSLHRVRNKNRQHLFVCSDRPVIIFSASKKLVFSNVNVKLVDTVCSLSSSAYRDCLVISDGNSMVFGTVDDIQKIHVRSIPMGESVLRIAYQKSTSTYGVCSNRTESKAERVFASKNALVTSQSRPKVASTRADMDESPPNTTSSFMVLDQNTFQVLHSHEFGPWETALSCISGQFTNDSSTYYVVGTGLIYPDETETKIGRIVVFEVDDVERSKLRRVHELVVRGSPLAIRILNGKLVAAINSSIRLFEWTTDKELRLECSSFNHVIALDLKVMNEEVAVADVMRSVSLLSYRMLEGNFEEVAKDWNSQWMVTCEFITAESILGGEAHLNLFTVEVDKTRPITDDGRYVLEPTGYWYLGELPKVMTRSTLVIQPEDSIIQYSQPIMFGTNQGTIGMIVQIDDKWKKFLIAIEKAIADSVKNCMHIEHSSYRTFVFQKRAEPPSGFVDGDLVESILDMDRSVAMDILSKVSDKGWDPSLPRDPVEILKVIEDLARMH

Target ORF information:

RefSeq Version	NM_069898.8
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA damage-binding protein 1 (ddb-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069898.8

ORF Insert Sequence:

ATGCCGATCA GTTACTGTGT TTCGGCAAAG AAGGCATCCG TTGTCGTAGA ATCTGTTGTC 
GGAAACTTCA CAGGACACGA AAATGTTAAC TTGATTGTGG CTCGTGGAAA TCGCATTGAT 
GTTCAACTAG TTTCACCGGA AGGACTCAAA AATGTCTGCG AGATCCCAAT CTACGGCCAA 
GTGTTGACAA TTGCACTTGT AAAATGTAAA CGCGATAAGC GTCATTCATT GATTGTTGTC 
ACCGAAAAAT GGCACATGGC AATTTTGGCG TATCGCGACG GAAAAGTTGT GACTCGTGCT 
GCTGGATGTA TTGCTGATCC GACTGGAAGA GCGACTGACA ATTTATTCAG TCTAACGATT 
CATCGAAACG GACTCATCGC TATTCGAGCT TTCGAGGGTA GTGTCAAAAT GATTCAATGG 
GAATCTGGTA CTGATCTACG TCATTTCAAT GTTAGATTTG ATTATCCAAA TGTCTCAGAC 
TTCAAGTTTG TTGACACTGG AGAAGATGAT GTATACCGAG TTGCCTTCAT CTATGACGAC 
GATCACGGAA AACATCTTCA ATTTTCCGAT CTTAACATGC ATGACAAGGA ATTCCGTACC 
TATTCCAGAC AAGCCAGTAT TGCTGCTGAT TCTTCGGTTT TAATTCCAGT TCCTCATGCT 
ATTGGAGGTG TTATAGTTCT CGGATCGAAC TCTGTCCTAT ACAAGCCAAA CGACAACCTC 
GGAGAAGTAG TTCCATACAC TTGCTCTTTG CTCGAGAACA CAACATTCAC ATGCCACGGA 
ATCGTTGATG CTTCTGGTGA AAGATTTCTT CTTTCCGATA CAGATGGACG TCTTCTAATG 
CTACTGTTGA ATGTAACTGA AAGTCAATCA GGATACACAG TTAAAGAAAT GCGTATCGAT 
TATCTTGGAG AAACTTCAAT TGCTGATTCC ATCAATTATA TTGACAATGG AGTAGTCTTT 
GTTGGTTCTC GACTCGGAGA TTCACAGCTT ATCAGGCTTA TGACTGAACC GAATGGAGGA 
AGTTATTCTG TGATACTTGA AACCTACAGT AACATTGGAC CAATTCGTGA TATGGTTATG 
GTAGAGTCTG ATGGACAACC ACAACTTGTA ACCTGTACTG GAGCTGACAA AGACGGATCA 
CTTCGTGTTA TTCGTAATGG AATCGGCATC GATGAATTGG CTAGTGTTGA TTTGGCTGGA 
GTCGTCGGCA TATTTCCCAT TCGTCTAGAT TCGAATGCTG ACAACTATGT AATTGTTTCA 
CTTTCTGATG AAACCCACGT TCTGCAAATT ACGGGAGAAG AGCTTGAAGA TGTGAAACTT 
TTGGAAATCA ACACAGATTT GCCAACAATT TTCGCGTCCA CTTTGTTTGG TCCAAACGAT 
TCTGGAATCA TTCTTCAAGC AACGGAAAAG CAGATTCGAC TTATGTCTTC TTCGGGACTT 
TCCAAATTTT GGGAACCAAC AAACGGTGAA ATTATTTCGA AAGTTTCTGT GAATGCAGCC 
AACGGACAAA TTGTTCTCGC AGCTAGAGAT ACAGTATACC TTCTCACTTG TATTGTTGAT 
GAAATGGGTG CTCTCGACAT TCAATTGACT GCTGAAAAGA AGTTTGAAAA CGAAATTGCA 
TGTTTGGACC TTTCGAATGA AGGAGACGAT CCTAACAATA AAGCAACCTT CTTGGTTTTA 
GCATTTTGGA GTACATTTGC GATGGAAGTA ATTCAGCTAC CTGATCTTAT AACTGTGTGC 
CACACGGATC TTCCAACAAA GATCATTCCT CGTTCGATCA TTGCAACATG CATAGAAGAA 
GTTCATTATC TTCTTGTTGC TTTCGGAGAC GGTGCACTTG TTTATTATGT GTTTGATATC 
AAAACAGGAA CACACGGTGA ACCAAAAAAG TCAAATGTCG GAACACGACC ACCGAGTCTT 
CATCGTGTAC GCAACAAGAA TCGACAACAC TTGTTCGTCT GTTCTGATCG TCCAGTGATC 
ATCTTCTCGG CCAGCAAAAA GCTTGTCTTC TCCAATGTTA ATGTTAAACT CGTCGATACT 
GTGTGCTCGC TGAGTTCCAG CGCCTATCGA GATTGTTTGG TCATTTCGGA TGGAAATTCG 
ATGGTTTTTG GCACAGTTGA CGATATTCAG AAGATCCACG TTCGCTCGAT TCCAATGGGA 
GAATCTGTTC TTCGTATTGC ATATCAAAAG AGCACAAGTA CATATGGAGT CTGCTCGAAT 
CGAACTGAAT CGAAGGCTGA ACGAGTTTTT GCTTCGAAAA ATGCACTTGT CACTTCTCAA 
AGTCGACCAA AAGTTGCATC CACCCGAGCT GACATGGATG AAAGTCCTCC AAATACCACA 
AGCAGTTTTA TGGTTCTCGA TCAAAACACA TTTCAAGTAC TTCATTCACA TGAATTCGGA 
CCATGGGAAA CTGCGTTGAG TTGCATCTCT GGACAATTCA CAAACGACTC GAGCACCTAC 
TACGTTGTTG GAACTGGTCT TATCTATCCA GACGAAACAG AGACGAAGAT CGGTAGAATC 
GTGGTATTTG AAGTTGACGA CGTGGAAAGA TCGAAACTTC GACGTGTACA TGAATTGGTT 
GTTCGTGGAA GTCCTTTAGC TATTCGAATT CTGAATGGAA AATTGGTTGC TGCAATTAAT 
AGCTCAATTA GACTATTCGA ATGGACAACT GATAAGGAAC TTCGACTGGA ATGCAGTAGC 
TTCAATCATG TCATTGCACT TGATCTTAAA GTTATGAATG AAGAGGTTGC AGTGGCTGAT 
GTGATGAGAT CTGTCTCTCT TCTCTCGTAC AGAATGCTGG AAGGAAATTT TGAGGAAGTT 
GCTAAAGATT GGAATAGTCA ATGGATGGTT ACTTGCGAGT TCATCACAGC TGAATCAATC 
CTTGGAGGAG AAGCTCATCT GAATCTGTTC ACTGTAGAAG TTGATAAAAC CCGTCCGATT 
ACTGATGATG GTCGATATGT GTTGGAACCA ACTGGATATT GGTATCTCGG AGAGCTTCCA 
AAAGTCATGA CAAGATCGAC TCTTGTCATT CAACCAGAAG ATAGTATTAT CCAGTACAGT 
CAACCAATCA TGTTTGGAAC AAATCAGGGA ACAATTGGAA TGATTGTGCA GATTGATGAC 
AAATGGAAAA AGTTTTTGAT TGCAATTGAA AAGGCTATCG CAGATTCCGT TAAAAACTGC 
ATGCACATTG AACATTCCAG TTACAGAACT TTTGTGTTCC AGAAAAGAGC GGAACCGCCA 
TCCGGATTCG TCGATGGAGA TCTCGTTGAA TCGATTCTCG ACATGGATCG TTCTGTTGCA 
ATGGATATCT TGAGTAAAGT ATCTGACAAA GGATGGGATC CAAGTCTGCC AAGAGATCCA 
GTTGAAATTC TCAAAGTCAT CGAGGATTTG GCGAGAATGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ddb-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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