trpa-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	trpa-1
Entrez Gene ID	178118
Full Name	TRPA cation channel homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_069848.4	NP_502249.3	Transient receptor potential cation channel subfamily A member 1 homolog
NM_069848.5	NP_502249.3	Transient receptor potential cation channel subfamily A member 1 homolog
NM_001307154.2	NP_001294083.1	ANK_REP_REGION domain-containing protein
NM_001307154.1	NP_001294083.1	Transient receptor potential cation channel subfamily A member 1 homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TRPA1	XP_002692772.2
Canis lupus familiaris (dog)	TRPA1	XP_544123.2
Caenorhabditis elegans (roundworm)	trpa-1	NP_502249.3
Pan troglodytes (chimpanzee)	TRPA1	XP_519806.2
Mus musculus (house mouse)	Trpa1	NP_808449.1
Danio rerio (zebrafish)	trpa1a	NP_001007066.1
Danio rerio (zebrafish)	trpa1b	NP_001007067.1
Drosophila melanogaster (fruit fly)	TrpA1	NP_648263.5
Homo sapiens (human)	TRPA1	NP_015628.2
	TRPA1	XP_001083172.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004863	XP_314325.4
Rattus norvegicus (Norway rat)	Trpa1	NP_997491.1
Gallus gallus (chicken)	TRPA1	XP_418294.4
Xenopus tropicalis (tropical clawed frog)	trpa1	NP_001121434.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reveals that TRPA-1, a cold-sensitive TRP channel, detects temperature drop in the environment to extend lifespan. This effect requires cold-induced, TRPA-1-mediated calcium influx and a calcium-sensitive PKC that signals to the transcription factor DAF-16/FOXO.
Title: A genetic program promotes C. elegans longevity at cold temperatures via a thermosensitive TRP channel.

harsh touch specifically required the DEG/ENaC proteins MEC-10 and DEGT-1 (F25D1.4), which represent putative components of a harsh touch mechanotransduction complex; responses to cold required the TRPA-1 channel and were MEC-10 and DEGT-1 independent
Title: Specific roles for DEG/ENaC and TRP channels in touch and thermosensation in C. elegans nociceptors.

This study demonstrates that trpa-1 encodes an ion channel that can be activated in response to mechanical pressure and is required for mechanosensory neuron function.
Title: Caenorhabditis elegans TRPA-1 functions in mechanosensation.

The following trpa-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpa-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342333	NM_069848.5 Latest version!	Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa343125	NM_001307154.2 Latest version!	Caenorhabditis elegans ANK_REP_REGION domain-containing protein (trpa-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa342333	NM_069848.4	Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa343125	NM_001307154.1	Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpa-1 ( NM_069848.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502249.3 Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), partial mRNA.

Price & Availability↑

CloneID	OCa342333
Clone ID Related Accession (Same CDS sequence)	NM_069848.4 , NM_069848.5
Accession Version	NM_069848.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Transient receptor potential cation channel subfamily A member 1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069848.3. COMPLETENESS: incomplete on both ends.

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAAAGATGGT CGTCAAGCTT CAATCTTTCG AGTCGCTGAA 
CTAGATGCTC GCACTGAAGC AGACAATCTT CGTTCAATAA TCCATCAAAG TGCTCGAGAA 
GGAAATGTAA ACGCTCTTCA AGAAGCTCTG CTGAAAGCTC CATTGGCAGT GAACGCCCAA 
GATGGTGATT TTATGACACC GCTTCACTAT GCAGCGAGAT ACGGAAACTA TGATGCAGTT 
AAACTATTAC TTTCTAAAAA TGCTCTACCT AATACAAAAA ATCGAGAAGG CGACACTCCG 
TTGCATATTG CTTCCAAGTA TATCTATGGA TACAGTGATA TTTGCTCAAT TATAGATGAG 
GATCAAGCTG ATTCCGCGAG AAAGTACAAT ACTGCAACCA AGAAGATCAT AAATGCCTTA 
GTTTCTGAAA ATGCTGAAAT CGATCCTGTG AATAAGTACC AATTGACTCC TCTTCACTAT 
GCAGCCATGA AATCCAACTT TTCTGCTCTA CACGCTCTCA TAAAACTGAA AGCAGATGTG 
GATGCGGAAG ATGATAACAA AATGACACCT CTTCTATTAG CCTGTGTTCA TGGAAGCCAA 
GAAATAATTC AAGAGCTTAT TAAAGCAAAT AGTAATGTTA CGAAAAGAGA TCAACGACTC 
AACACAGTAT TTCATATTGT TGCATTGCGG GGTGAGCCGG AATACTTAGA GATGATGATG 
GATCATGATC CAGTTGAAGC AATCAAGGCA TTAAATTTAT TTAATAATGA AAAGAAGACA 
CCTTTGAGAA TGGCTGTAGA AGGAAATCAT CCGGAAACTT TGAAAAAGAT TCTGCAGATG 
GAAAAGAAAA ATTCATGTAA ATGGATGGAT AGGGAGAAGG AATTGATCCA TTTTGCCGCT 
GAAAAGGGAT TTTTAGAAGT TTTAAAAGCA TTAGTGGAAG CTGGAGGAAA CAAGAATGAA 
CTGAATGAAG TTAAAGCAGT GCCACTTCAC GTTGCTGCTC AAATGAATCA ATTGGAAGTT 
GTAAGCTATT TAATAGAAGA GGAAAAGGAT AATATTGATG TAGTCGATGA ACAAGGCTTG 
ACTCCCTTGA TGATGGCAGT CACTCATGAT AGTAAAAAGT GTGTTGAGTA CCTGATTGCA 
AAAAAAGCAA ACTTGACAAT CACGGATAAA GATGAACGTA CCCCAGTATT CATTGGAGCC 
AAGTTCAATG CTCTTTCTTC TGTTGAATAC ATTTTGGACC ACCTTAGAAA AAAAAATAAA 
GAGACTGAAA GAAGTGCTTT AAAGTCACCT ACTCGAAATA CACTTCGGAT TGTGTCTGAA 
GACGTGAGGA GGACTATGGT CAATATGGTA GATCGAGATC AAAACACTCC AATGCATATT 
GTTGCTTCAA ATGGATATTT GGAAATGATG CAGTTGCTCC AAAAACACGG AGCATCAATT 
ACTCAAGTGA ACGAAGATGA GGAGACTGCA TTGCACAGAG CTGCAATTGG GGGTCAAACT 
GGAGCAGTAA GACAACTACT TGAATGGGAT ATTCGACTTT TGTTGATGAA AGATGAAATG 
GGAAATAGTG CATTGCATTT GGCAGCCAGA AGTGGACACG ATGCCACAAC AAAAGTTTTA 
TTGGACAACG GTGCTGATAA GGAAGCAAAG AATTCGTACC AAAAAACTCC GTTACAAGTA 
GCCGTGGATT CTGGAAAATT GGAAACCTGT CAGAGATTAG TGGCAAAGGG AGCACAGATT 
GAGAGCTCTA GTGATACAAA AACAGTATTG CATACAGCTG CATTCTATGG TAATGAATCT 
ATCGTTAGAT ACTTTATCGC TGAGGGTGTC ACAATTGATA GAAGAGATGA AGAAGGAAAA 
ACTGCATTTG ATATAGCATG TGAGAATGAT CATAAGGATG TGGCACGAGC ATTTCTAGAA 
ACGGATCAAT GGAAAAACTT GATGATTCCA TGTGATGTGA TTCCATTGGA TAAGCACAGA 
AATCCTGTAA ATATGAAAAG GAGAACACCC TTCAGAACTC TTCTGACAAA GTTTCCAGAA 
CTAGCTTCGT TTGTTATGGA CAACTGTATA GAAAAAAGCA AAGAAGAAAC CGATTCAACT 
CAAAGTGTTG CATACAATTT TGAATTTCTC GATGATACTT ACATGATGAG ATGTGTGAGT 
GATGATGGAA CAGGAGAACA ATTAATTGGA TGCAAATCTG CATATGATGA AGATTTCAAA 
CTGGAAAAGG ATGCACAATC CTATGCTTCC AATTACGACC GCGTCTATAA ATACCATCCA 
TTGAAGCTAA TGGCAGACGC TGAAAAACTG CATCTTCTCA ACCATCCACT ATCAAAAGCC 
CTTCTCAAAT ACAAATGGAA TCGGCTGGGG AGACCAATGT ATTATTTTGC TCTTTTCATG 
TATCTTGTTT TTATTGTCAG TTTGACTCAA TATGTAAGAC ATACAAAGGC TCCATACAAT 
GTTTGGAATG AGGAATCGTA TTATGACAGT GAATACTTCG ATGAAAACGA GACGTGCCCT 
CAGATTAACA CCACAAAACC TGACGTCGTA TGGAAAATCA TCATTCAAAC TTTAGCCGTC 
TGTCAAATTC TAGTGGAATG TTTCCAATTA TTCCAACGAA AATTTGCATA TCTGGTAAAC 
TGGGAGAACT GGATTGATTG TTTTATATAC TCAACAGCCC TTATTACTGT ATACGACTTC 
TCCGAATGCT CAGCAACATC AGGAGTTCGT CAGAACTGGC AATGGATTCT AGCAGCTTTA 
TGCATATTTT TCGGATGGAT CAACTTATTG TTCATGATCA GAAAAATGCC AAGATTTGGA 
ATATTCGTGG TGATGTTTGT GGATATTGTG AAGACATTCT TCCGCTTCTT CCCAGTTTTT 
GTGCTATTCA TAATTGCGTT CTCTTCATCC TTCTACGTGA TCCTTCAAAA TAGACCGGAA 
TTTTCCACGA TCTTCATGTC TCCTCTCAAA ACTACAGTAA TGATGATTGG AGAATTTGAG 
TTCACTGGAA TATTTCACGG AGATGAAACT ACTCATGCTG AGAAAATGTT TGGCCCAGCA 
CACACAGCAG TCGCATGTGC ACTTTTCTTC TTTTTCTGCA TAATAATGAC AATTCTCCTG 
ATGAATCTAC TTGTTGGTTT GGCAGTTGAT GATATTAAAG GTGTACAAGA AAAAGCTGAA 
CTGAAACGTC TCGCAATGCA AGTTGATCTT GTTCTGCAAA TTGAGGCCTC TCTTCACTTT 
TTCATTCAAC GAACCAAAAA ATATGCGACT TGCCGTTATG CAACTTTTCC ATACGGAAAA 
CTTCATAAGA CTGGTTTTGC CGGATGGTGG TCCAATTTCA GAAGAAGATT CGGGTTGAGT 
GTCAGCACTG ATCCAGAAAT TGACGAGATG TATGAACGGG AAGCGGAGTT CACTTCTGAA 
ATGACTCAAA AACTTCAAAA CCAAGCAGCT AAACTCAAAA ATATTCAAGA AAATATTGAT 
GTGATGTACG AGAAGCAAGT ACGACTTGAA GCAATCATTG CAAAGCTCGC GACAGGGCTC 
AACATAAATA TTGAACTTGA GGAGAAAGAT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502249.3
CDS	1..3636
Translation	MSKKSLGLDVRLELEGLISNDDTIRSEKDGRQASIFRVAELDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTKTVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCIEKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTKPDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIYSTALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQRTKKYATCRYATFPYGKLHKTGFAGWWSNFRRRFGLSVSTDPEIDEMYEREAEFTSEMTQKLQNQAAKLKNIQENIDVMYEKQVRLEAIIAKLATGLNINIELEEKDN

Target ORF information:

RefSeq Version	NM_069848.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069848.4

ORF Insert Sequence:

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAAAGATGGT CGTCAAGCTT CAATCTTTCG AGTCGCTGAA 
CTAGATGCTC GCACTGAAGC AGACAATCTT CGTTCAATAA TCCATCAAAG TGCTCGAGAA 
GGAAATGTAA ACGCTCTTCA AGAAGCTCTG CTGAAAGCTC CATTGGCAGT GAACGCCCAA 
GATGGTGATT TTATGACACC GCTTCACTAT GCAGCGAGAT ACGGAAACTA TGATGCAGTT 
AAACTATTAC TTTCTAAAAA TGCTCTACCT AATACAAAAA ATCGAGAAGG CGACACTCCG 
TTGCATATTG CTTCCAAGTA TATCTATGGA TACAGTGATA TTTGCTCAAT TATAGATGAG 
GATCAAGCTG ATTCCGCGAG AAAGTACAAT ACTGCAACCA AGAAGATCAT AAATGCCTTA 
GTTTCTGAAA ATGCTGAAAT CGATCCTGTG AATAAGTACC AATTGACTCC TCTTCACTAT 
GCAGCCATGA AATCCAACTT TTCTGCTCTA CACGCTCTCA TAAAACTGAA AGCAGATGTG 
GATGCGGAAG ATGATAACAA AATGACACCT CTTCTATTAG CCTGTGTTCA TGGAAGCCAA 
GAAATAATTC AAGAGCTTAT TAAAGCAAAT AGTAATGTTA CGAAAAGAGA TCAACGACTC 
AACACAGTAT TTCATATTGT TGCATTGCGG GGTGAGCCGG AATACTTAGA GATGATGATG 
GATCATGATC CAGTTGAAGC AATCAAGGCA TTAAATTTAT TTAATAATGA AAAGAAGACA 
CCTTTGAGAA TGGCTGTAGA AGGAAATCAT CCGGAAACTT TGAAAAAGAT TCTGCAGATG 
GAAAAGAAAA ATTCATGTAA ATGGATGGAT AGGGAGAAGG AATTGATCCA TTTTGCCGCT 
GAAAAGGGAT TTTTAGAAGT TTTAAAAGCA TTAGTGGAAG CTGGAGGAAA CAAGAATGAA 
CTGAATGAAG TTAAAGCAGT GCCACTTCAC GTTGCTGCTC AAATGAATCA ATTGGAAGTT 
GTAAGCTATT TAATAGAAGA GGAAAAGGAT AATATTGATG TAGTCGATGA ACAAGGCTTG 
ACTCCCTTGA TGATGGCAGT CACTCATGAT AGTAAAAAGT GTGTTGAGTA CCTGATTGCA 
AAAAAAGCAA ACTTGACAAT CACGGATAAA GATGAACGTA CCCCAGTATT CATTGGAGCC 
AAGTTCAATG CTCTTTCTTC TGTTGAATAC ATTTTGGACC ACCTTAGAAA AAAAAATAAA 
GAGACTGAAA GAAGTGCTTT AAAGTCACCT ACTCGAAATA CACTTCGGAT TGTGTCTGAA 
GACGTGAGGA GGACTATGGT CAATATGGTA GATCGAGATC AAAACACTCC AATGCATATT 
GTTGCTTCAA ATGGATATTT GGAAATGATG CAGTTGCTCC AAAAACACGG AGCATCAATT 
ACTCAAGTGA ACGAAGATGA GGAGACTGCA TTGCACAGAG CTGCAATTGG GGGTCAAACT 
GGAGCAGTAA GACAACTACT TGAATGGGAT ATTCGACTTT TGTTGATGAA AGATGAAATG 
GGAAATAGTG CATTGCATTT GGCAGCCAGA AGTGGACACG ATGCCACAAC AAAAGTTTTA 
TTGGACAACG GTGCTGATAA GGAAGCAAAG AATTCGTACC AAAAAACTCC GTTACAAGTA 
GCCGTGGATT CTGGAAAATT GGAAACCTGT CAGAGATTAG TGGCAAAGGG AGCACAGATT 
GAGAGCTCTA GTGATACAAA AACAGTATTG CATACAGCTG CATTCTATGG TAATGAATCT 
ATCGTTAGAT ACTTTATCGC TGAGGGTGTC ACAATTGATA GAAGAGATGA AGAAGGAAAA 
ACTGCATTTG ATATAGCATG TGAGAATGAT CATAAGGATG TGGCACGAGC ATTTCTAGAA 
ACGGATCAAT GGAAAAACTT GATGATTCCA TGTGATGTGA TTCCATTGGA TAAGCACAGA 
AATCCTGTAA ATATGAAAAG GAGAACACCC TTCAGAACTC TTCTGACAAA GTTTCCAGAA 
CTAGCTTCGT TTGTTATGGA CAACTGTATA GAAAAAAGCA AAGAAGAAAC CGATTCAACT 
CAAAGTGTTG CATACAATTT TGAATTTCTC GATGATACTT ACATGATGAG ATGTGTGAGT 
GATGATGGAA CAGGAGAACA ATTAATTGGA TGCAAATCTG CATATGATGA AGATTTCAAA 
CTGGAAAAGG ATGCACAATC CTATGCTTCC AATTACGACC GCGTCTATAA ATACCATCCA 
TTGAAGCTAA TGGCAGACGC TGAAAAACTG CATCTTCTCA ACCATCCACT ATCAAAAGCC 
CTTCTCAAAT ACAAATGGAA TCGGCTGGGG AGACCAATGT ATTATTTTGC TCTTTTCATG 
TATCTTGTTT TTATTGTCAG TTTGACTCAA TATGTAAGAC ATACAAAGGC TCCATACAAT 
GTTTGGAATG AGGAATCGTA TTATGACAGT GAATACTTCG ATGAAAACGA GACGTGCCCT 
CAGATTAACA CCACAAAACC TGACGTCGTA TGGAAAATCA TCATTCAAAC TTTAGCCGTC 
TGTCAAATTC TAGTGGAATG TTTCCAATTA TTCCAACGAA AATTTGCATA TCTGGTAAAC 
TGGGAGAACT GGATTGATTG TTTTATATAC TCAACAGCCC TTATTACTGT ATACGACTTC 
TCCGAATGCT CAGCAACATC AGGAGTTCGT CAGAACTGGC AATGGATTCT AGCAGCTTTA 
TGCATATTTT TCGGATGGAT CAACTTATTG TTCATGATCA GAAAAATGCC AAGATTTGGA 
ATATTCGTGG TGATGTTTGT GGATATTGTG AAGACATTCT TCCGCTTCTT CCCAGTTTTT 
GTGCTATTCA TAATTGCGTT CTCTTCATCC TTCTACGTGA TCCTTCAAAA TAGACCGGAA 
TTTTCCACGA TCTTCATGTC TCCTCTCAAA ACTACAGTAA TGATGATTGG AGAATTTGAG 
TTCACTGGAA TATTTCACGG AGATGAAACT ACTCATGCTG AGAAAATGTT TGGCCCAGCA 
CACACAGCAG TCGCATGTGC ACTTTTCTTC TTTTTCTGCA TAATAATGAC AATTCTCCTG 
ATGAATCTAC TTGTTGGTTT GGCAGTTGAT GATATTAAAG GTGTACAAGA AAAAGCTGAA 
CTGAAACGTC TCGCAATGCA AGTTGATCTT GTTCTGCAAA TTGAGGCCTC TCTTCACTTT 
TTCATTCAAC GAACCAAAAA ATATGCGACT TGCCGTTATG CAACTTTTCC ATACGGAAAA 
CTTCATAAGA CTGGTTTTGC CGGATGGTGG TCCAATTTCA GAAGAAGATT CGGGTTGAGT 
GTCAGCACTG ATCCAGAAAT TGACGAGATG TATGAACGGG AAGCGGAGTT CACTTCTGAA 
ATGACTCAAA AACTTCAAAA CCAAGCAGCT AAACTCAAAA ATATTCAAGA AAATATTGAT 
GTGATGTACG AGAAGCAAGT ACGACTTGAA GCAATCATTG CAAAGCTCGC GACAGGGCTC 
AACATAAATA TTGAACTTGA GGAGAAAGAT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpa-1 ( NM_069848.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502249.3 Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), mRNA.

Price & Availability↑

CloneID	OCa342333
Clone ID Related Accession (Same CDS sequence)	NM_069848.4 , NM_069848.5
Accession Version	NM_069848.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Transient receptor potential cation channel subfamily A member 1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069848.4.

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAAAGATGGT CGTCAAGCTT CAATCTTTCG AGTCGCTGAA 
CTAGATGCTC GCACTGAAGC AGACAATCTT CGTTCAATAA TCCATCAAAG TGCTCGAGAA 
GGAAATGTAA ACGCTCTTCA AGAAGCTCTG CTGAAAGCTC CATTGGCAGT GAACGCCCAA 
GATGGTGATT TTATGACACC GCTTCACTAT GCAGCGAGAT ACGGAAACTA TGATGCAGTT 
AAACTATTAC TTTCTAAAAA TGCTCTACCT AATACAAAAA ATCGAGAAGG CGACACTCCG 
TTGCATATTG CTTCCAAGTA TATCTATGGA TACAGTGATA TTTGCTCAAT TATAGATGAG 
GATCAAGCTG ATTCCGCGAG AAAGTACAAT ACTGCAACCA AGAAGATCAT AAATGCCTTA 
GTTTCTGAAA ATGCTGAAAT CGATCCTGTG AATAAGTACC AATTGACTCC TCTTCACTAT 
GCAGCCATGA AATCCAACTT TTCTGCTCTA CACGCTCTCA TAAAACTGAA AGCAGATGTG 
GATGCGGAAG ATGATAACAA AATGACACCT CTTCTATTAG CCTGTGTTCA TGGAAGCCAA 
GAAATAATTC AAGAGCTTAT TAAAGCAAAT AGTAATGTTA CGAAAAGAGA TCAACGACTC 
AACACAGTAT TTCATATTGT TGCATTGCGG GGTGAGCCGG AATACTTAGA GATGATGATG 
GATCATGATC CAGTTGAAGC AATCAAGGCA TTAAATTTAT TTAATAATGA AAAGAAGACA 
CCTTTGAGAA TGGCTGTAGA AGGAAATCAT CCGGAAACTT TGAAAAAGAT TCTGCAGATG 
GAAAAGAAAA ATTCATGTAA ATGGATGGAT AGGGAGAAGG AATTGATCCA TTTTGCCGCT 
GAAAAGGGAT TTTTAGAAGT TTTAAAAGCA TTAGTGGAAG CTGGAGGAAA CAAGAATGAA 
CTGAATGAAG TTAAAGCAGT GCCACTTCAC GTTGCTGCTC AAATGAATCA ATTGGAAGTT 
GTAAGCTATT TAATAGAAGA GGAAAAGGAT AATATTGATG TAGTCGATGA ACAAGGCTTG 
ACTCCCTTGA TGATGGCAGT CACTCATGAT AGTAAAAAGT GTGTTGAGTA CCTGATTGCA 
AAAAAAGCAA ACTTGACAAT CACGGATAAA GATGAACGTA CCCCAGTATT CATTGGAGCC 
AAGTTCAATG CTCTTTCTTC TGTTGAATAC ATTTTGGACC ACCTTAGAAA AAAAAATAAA 
GAGACTGAAA GAAGTGCTTT AAAGTCACCT ACTCGAAATA CACTTCGGAT TGTGTCTGAA 
GACGTGAGGA GGACTATGGT CAATATGGTA GATCGAGATC AAAACACTCC AATGCATATT 
GTTGCTTCAA ATGGATATTT GGAAATGATG CAGTTGCTCC AAAAACACGG AGCATCAATT 
ACTCAAGTGA ACGAAGATGA GGAGACTGCA TTGCACAGAG CTGCAATTGG GGGTCAAACT 
GGAGCAGTAA GACAACTACT TGAATGGGAT ATTCGACTTT TGTTGATGAA AGATGAAATG 
GGAAATAGTG CATTGCATTT GGCAGCCAGA AGTGGACACG ATGCCACAAC AAAAGTTTTA 
TTGGACAACG GTGCTGATAA GGAAGCAAAG AATTCGTACC AAAAAACTCC GTTACAAGTA 
GCCGTGGATT CTGGAAAATT GGAAACCTGT CAGAGATTAG TGGCAAAGGG AGCACAGATT 
GAGAGCTCTA GTGATACAAA AACAGTATTG CATACAGCTG CATTCTATGG TAATGAATCT 
ATCGTTAGAT ACTTTATCGC TGAGGGTGTC ACAATTGATA GAAGAGATGA AGAAGGAAAA 
ACTGCATTTG ATATAGCATG TGAGAATGAT CATAAGGATG TGGCACGAGC ATTTCTAGAA 
ACGGATCAAT GGAAAAACTT GATGATTCCA TGTGATGTGA TTCCATTGGA TAAGCACAGA 
AATCCTGTAA ATATGAAAAG GAGAACACCC TTCAGAACTC TTCTGACAAA GTTTCCAGAA 
CTAGCTTCGT TTGTTATGGA CAACTGTATA GAAAAAAGCA AAGAAGAAAC CGATTCAACT 
CAAAGTGTTG CATACAATTT TGAATTTCTC GATGATACTT ACATGATGAG ATGTGTGAGT 
GATGATGGAA CAGGAGAACA ATTAATTGGA TGCAAATCTG CATATGATGA AGATTTCAAA 
CTGGAAAAGG ATGCACAATC CTATGCTTCC AATTACGACC GCGTCTATAA ATACCATCCA 
TTGAAGCTAA TGGCAGACGC TGAAAAACTG CATCTTCTCA ACCATCCACT ATCAAAAGCC 
CTTCTCAAAT ACAAATGGAA TCGGCTGGGG AGACCAATGT ATTATTTTGC TCTTTTCATG 
TATCTTGTTT TTATTGTCAG TTTGACTCAA TATGTAAGAC ATACAAAGGC TCCATACAAT 
GTTTGGAATG AGGAATCGTA TTATGACAGT GAATACTTCG ATGAAAACGA GACGTGCCCT 
CAGATTAACA CCACAAAACC TGACGTCGTA TGGAAAATCA TCATTCAAAC TTTAGCCGTC 
TGTCAAATTC TAGTGGAATG TTTCCAATTA TTCCAACGAA AATTTGCATA TCTGGTAAAC 
TGGGAGAACT GGATTGATTG TTTTATATAC TCAACAGCCC TTATTACTGT ATACGACTTC 
TCCGAATGCT CAGCAACATC AGGAGTTCGT CAGAACTGGC AATGGATTCT AGCAGCTTTA 
TGCATATTTT TCGGATGGAT CAACTTATTG TTCATGATCA GAAAAATGCC AAGATTTGGA 
ATATTCGTGG TGATGTTTGT GGATATTGTG AAGACATTCT TCCGCTTCTT CCCAGTTTTT 
GTGCTATTCA TAATTGCGTT CTCTTCATCC TTCTACGTGA TCCTTCAAAA TAGACCGGAA 
TTTTCCACGA TCTTCATGTC TCCTCTCAAA ACTACAGTAA TGATGATTGG AGAATTTGAG 
TTCACTGGAA TATTTCACGG AGATGAAACT ACTCATGCTG AGAAAATGTT TGGCCCAGCA 
CACACAGCAG TCGCATGTGC ACTTTTCTTC TTTTTCTGCA TAATAATGAC AATTCTCCTG 
ATGAATCTAC TTGTTGGTTT GGCAGTTGAT GATATTAAAG GTGTACAAGA AAAAGCTGAA 
CTGAAACGTC TCGCAATGCA AGTTGATCTT GTTCTGCAAA TTGAGGCCTC TCTTCACTTT 
TTCATTCAAC GAACCAAAAA ATATGCGACT TGCCGTTATG CAACTTTTCC ATACGGAAAA 
CTTCATAAGA CTGGTTTTGC CGGATGGTGG TCCAATTTCA GAAGAAGATT CGGGTTGAGT 
GTCAGCACTG ATCCAGAAAT TGACGAGATG TATGAACGGG AAGCGGAGTT CACTTCTGAA 
ATGACTCAAA AACTTCAAAA CCAAGCAGCT AAACTCAAAA ATATTCAAGA AAATATTGAT 
GTGATGTACG AGAAGCAAGT ACGACTTGAA GCAATCATTG CAAAGCTCGC GACAGGGCTC 
AACATAAATA TTGAACTTGA GGAGAAAGAT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502249.3
CDS	16..3651
Translation	MSKKSLGLDVRLELEGLISNDDTIRSEKDGRQASIFRVAELDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTKTVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCIEKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTKPDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIYSTALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQRTKKYATCRYATFPYGKLHKTGFAGWWSNFRRRFGLSVSTDPEIDEMYEREAEFTSEMTQKLQNQAAKLKNIQENIDVMYEKQVRLEAIIAKLATGLNINIELEEKDN

Target ORF information:

RefSeq Version	NM_069848.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069848.5

ORF Insert Sequence:

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAAAGATGGT CGTCAAGCTT CAATCTTTCG AGTCGCTGAA 
CTAGATGCTC GCACTGAAGC AGACAATCTT CGTTCAATAA TCCATCAAAG TGCTCGAGAA 
GGAAATGTAA ACGCTCTTCA AGAAGCTCTG CTGAAAGCTC CATTGGCAGT GAACGCCCAA 
GATGGTGATT TTATGACACC GCTTCACTAT GCAGCGAGAT ACGGAAACTA TGATGCAGTT 
AAACTATTAC TTTCTAAAAA TGCTCTACCT AATACAAAAA ATCGAGAAGG CGACACTCCG 
TTGCATATTG CTTCCAAGTA TATCTATGGA TACAGTGATA TTTGCTCAAT TATAGATGAG 
GATCAAGCTG ATTCCGCGAG AAAGTACAAT ACTGCAACCA AGAAGATCAT AAATGCCTTA 
GTTTCTGAAA ATGCTGAAAT CGATCCTGTG AATAAGTACC AATTGACTCC TCTTCACTAT 
GCAGCCATGA AATCCAACTT TTCTGCTCTA CACGCTCTCA TAAAACTGAA AGCAGATGTG 
GATGCGGAAG ATGATAACAA AATGACACCT CTTCTATTAG CCTGTGTTCA TGGAAGCCAA 
GAAATAATTC AAGAGCTTAT TAAAGCAAAT AGTAATGTTA CGAAAAGAGA TCAACGACTC 
AACACAGTAT TTCATATTGT TGCATTGCGG GGTGAGCCGG AATACTTAGA GATGATGATG 
GATCATGATC CAGTTGAAGC AATCAAGGCA TTAAATTTAT TTAATAATGA AAAGAAGACA 
CCTTTGAGAA TGGCTGTAGA AGGAAATCAT CCGGAAACTT TGAAAAAGAT TCTGCAGATG 
GAAAAGAAAA ATTCATGTAA ATGGATGGAT AGGGAGAAGG AATTGATCCA TTTTGCCGCT 
GAAAAGGGAT TTTTAGAAGT TTTAAAAGCA TTAGTGGAAG CTGGAGGAAA CAAGAATGAA 
CTGAATGAAG TTAAAGCAGT GCCACTTCAC GTTGCTGCTC AAATGAATCA ATTGGAAGTT 
GTAAGCTATT TAATAGAAGA GGAAAAGGAT AATATTGATG TAGTCGATGA ACAAGGCTTG 
ACTCCCTTGA TGATGGCAGT CACTCATGAT AGTAAAAAGT GTGTTGAGTA CCTGATTGCA 
AAAAAAGCAA ACTTGACAAT CACGGATAAA GATGAACGTA CCCCAGTATT CATTGGAGCC 
AAGTTCAATG CTCTTTCTTC TGTTGAATAC ATTTTGGACC ACCTTAGAAA AAAAAATAAA 
GAGACTGAAA GAAGTGCTTT AAAGTCACCT ACTCGAAATA CACTTCGGAT TGTGTCTGAA 
GACGTGAGGA GGACTATGGT CAATATGGTA GATCGAGATC AAAACACTCC AATGCATATT 
GTTGCTTCAA ATGGATATTT GGAAATGATG CAGTTGCTCC AAAAACACGG AGCATCAATT 
ACTCAAGTGA ACGAAGATGA GGAGACTGCA TTGCACAGAG CTGCAATTGG GGGTCAAACT 
GGAGCAGTAA GACAACTACT TGAATGGGAT ATTCGACTTT TGTTGATGAA AGATGAAATG 
GGAAATAGTG CATTGCATTT GGCAGCCAGA AGTGGACACG ATGCCACAAC AAAAGTTTTA 
TTGGACAACG GTGCTGATAA GGAAGCAAAG AATTCGTACC AAAAAACTCC GTTACAAGTA 
GCCGTGGATT CTGGAAAATT GGAAACCTGT CAGAGATTAG TGGCAAAGGG AGCACAGATT 
GAGAGCTCTA GTGATACAAA AACAGTATTG CATACAGCTG CATTCTATGG TAATGAATCT 
ATCGTTAGAT ACTTTATCGC TGAGGGTGTC ACAATTGATA GAAGAGATGA AGAAGGAAAA 
ACTGCATTTG ATATAGCATG TGAGAATGAT CATAAGGATG TGGCACGAGC ATTTCTAGAA 
ACGGATCAAT GGAAAAACTT GATGATTCCA TGTGATGTGA TTCCATTGGA TAAGCACAGA 
AATCCTGTAA ATATGAAAAG GAGAACACCC TTCAGAACTC TTCTGACAAA GTTTCCAGAA 
CTAGCTTCGT TTGTTATGGA CAACTGTATA GAAAAAAGCA AAGAAGAAAC CGATTCAACT 
CAAAGTGTTG CATACAATTT TGAATTTCTC GATGATACTT ACATGATGAG ATGTGTGAGT 
GATGATGGAA CAGGAGAACA ATTAATTGGA TGCAAATCTG CATATGATGA AGATTTCAAA 
CTGGAAAAGG ATGCACAATC CTATGCTTCC AATTACGACC GCGTCTATAA ATACCATCCA 
TTGAAGCTAA TGGCAGACGC TGAAAAACTG CATCTTCTCA ACCATCCACT ATCAAAAGCC 
CTTCTCAAAT ACAAATGGAA TCGGCTGGGG AGACCAATGT ATTATTTTGC TCTTTTCATG 
TATCTTGTTT TTATTGTCAG TTTGACTCAA TATGTAAGAC ATACAAAGGC TCCATACAAT 
GTTTGGAATG AGGAATCGTA TTATGACAGT GAATACTTCG ATGAAAACGA GACGTGCCCT 
CAGATTAACA CCACAAAACC TGACGTCGTA TGGAAAATCA TCATTCAAAC TTTAGCCGTC 
TGTCAAATTC TAGTGGAATG TTTCCAATTA TTCCAACGAA AATTTGCATA TCTGGTAAAC 
TGGGAGAACT GGATTGATTG TTTTATATAC TCAACAGCCC TTATTACTGT ATACGACTTC 
TCCGAATGCT CAGCAACATC AGGAGTTCGT CAGAACTGGC AATGGATTCT AGCAGCTTTA 
TGCATATTTT TCGGATGGAT CAACTTATTG TTCATGATCA GAAAAATGCC AAGATTTGGA 
ATATTCGTGG TGATGTTTGT GGATATTGTG AAGACATTCT TCCGCTTCTT CCCAGTTTTT 
GTGCTATTCA TAATTGCGTT CTCTTCATCC TTCTACGTGA TCCTTCAAAA TAGACCGGAA 
TTTTCCACGA TCTTCATGTC TCCTCTCAAA ACTACAGTAA TGATGATTGG AGAATTTGAG 
TTCACTGGAA TATTTCACGG AGATGAAACT ACTCATGCTG AGAAAATGTT TGGCCCAGCA 
CACACAGCAG TCGCATGTGC ACTTTTCTTC TTTTTCTGCA TAATAATGAC AATTCTCCTG 
ATGAATCTAC TTGTTGGTTT GGCAGTTGAT GATATTAAAG GTGTACAAGA AAAAGCTGAA 
CTGAAACGTC TCGCAATGCA AGTTGATCTT GTTCTGCAAA TTGAGGCCTC TCTTCACTTT 
TTCATTCAAC GAACCAAAAA ATATGCGACT TGCCGTTATG CAACTTTTCC ATACGGAAAA 
CTTCATAAGA CTGGTTTTGC CGGATGGTGG TCCAATTTCA GAAGAAGATT CGGGTTGAGT 
GTCAGCACTG ATCCAGAAAT TGACGAGATG TATGAACGGG AAGCGGAGTT CACTTCTGAA 
ATGACTCAAA AACTTCAAAA CCAAGCAGCT AAACTCAAAA ATATTCAAGA AAATATTGAT 
GTGATGTACG AGAAGCAAGT ACGACTTGAA GCAATCATTG CAAAGCTCGC GACAGGGCTC 
AACATAAATA TTGAACTTGA GGAGAAAGAT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpa-1 ( NM_001307154.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294083.1 Caenorhabditis elegans ANK_REP_REGION domain-containing protein (trpa-1), partial mRNA.

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CloneID	OCa343125
Clone ID Related Accession (Same CDS sequence)	NM_001307154.2 , NM_001307154.1
Accession Version	NM_001307154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ANK_REP_REGION domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307154.1. COMPLETENESS: incomplete on the 5' end.

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAATGGTCGT CAAGCTTCAA TCTTTCGAGT CGCTGAACTA 
GATGCTCGCA CTGAAGCAGA CAATCTTCGT TCAATAATCC ATCAAAGTGC TCGAGAAGGA 
AATGTAAACG CTCTTCAAGA AGCTCTGCTG AAAGCTCCAT TGGCAGTGAA CGCCCAAGAT 
GGTGATTTTA TGACACCGCT TCACTATGCA GCGAGATACG GAAACTATGA TGCAGTTAAA 
CTATTACTTT CTAAAAATGC TCTACCTAAT ACAAAAAATC GAGAAGGCGA CACTCCGTTG 
CATATTGCTT CCAAGTATAT CTATGGATAC AGTGATATTT GCTCAATTAT AGATGAGGAT 
CAAGCTGATT CCGCGAGAAA GTACAATACT GCAACCAAGA AGATCATAAA TGCCTTAGTT 
TCTGAAAATG CTGAAATCGA TCCTGTGAAT AAGTACCAAT TGACTCCTCT TCACTATGCA 
GCCATGAAAT CCAACTTTTC TGCTCTACAC GCTCTCATAA AACTGAAAGC AGATGTGGAT 
GCGGAAGATG ATAACAAAAT GACACCTCTT CTATTAGCCT GTGTTCATGG AAGCCAAGAA 
ATAATTCAAG AGCTTATTAA AGCAAATAGT AATGTTACGA AAAGAGATCA ACGACTCAAC 
ACAGTATTTC ATATTGTTGC ATTGCGGGGT GAGCCGGAAT ACTTAGAGAT GATGATGGAT 
CATGATCCAG TTGAAGCAAT CAAGGCATTA AATTTATTTA ATAATGAAAA GAAGACACCT 
TTGAGAATGG CTGTAGAAGG AAATCATCCG GAAACTTTGA AAAAGATTCT GCAGATGGAA 
AAGAAAAATT CATGTAAATG GATGGATAGG GAGAAGGAAT TGATCCATTT TGCCGCTGAA 
AAGGGATTTT TAGAAGTTTT AAAAGCATTA GTGGAAGCTG GAGGAAACAA GAATGAACTG 
AATGAAGTTA AAGCAGTGCC ACTTCACGTT GCTGCTCAAA TGAATCAATT GGAAGTTGTA 
AGCTATTTAA TAGAAGAGGA AAAGGATAAT ATTGATGTAG TCGATGAACA AGGCTTGACT 
CCCTTGATGA TGGCAGTCAC TCATGATAGT AAAAAGTGTG TTGAGTACCT GATTGCAAAA 
AAAGCAAACT TGACAATCAC GGATAAAGAT GAACGTACCC CAGTATTCAT TGGAGCCAAG 
TTCAATGCTC TTTCTTCTGT TGAATACATT TTGGACCACC TTAGAAAAAA AAATAAAGAG 
ACTGAAAGAA GTGCTTTAAA GTCACCTACT CGAAATACAC TTCGGATTGT GTCTGAAGAC 
GTGAGGAGGA CTATGGTCAA TATGGTAGAT CGAGATCAAA ACACTCCAAT GCATATTGTT 
GCTTCAAATG GATATTTGGA AATGATGCAG TTGCTCCAAA AACACGGAGC ATCAATTACT 
CAAGTGAACG AAGATGAGGA GACTGCATTG CACAGAGCTG CAATTGGGGG TCAAACTGGA 
GCAGTAAGAC AACTACTTGA ATGGGATATT CGACTTTTGT TGATGAAAGA TGAAATGGGA 
AATAGTGCAT TGCATTTGGC AGCCAGAAGT GGACACGATG CCACAACAAA AGTTTTATTG 
GACAACGGTG CTGATAAGGA AGCAAAGAAT TCGTACCAAA AAACTCCGTT ACAAGTAGCC 
GTGGATTCTG GAAAATTGGA AACCTGTCAG AGATTAGTGG CAAAGGGAGC ACAGATTGAG 
AGCTCTAGTG ATACAAAAAC AGTATTGCAT ACAGCTGCAT TCTATGGTAA TGAATCTATC 
GTTAGATACT TTATCGCTGA GGGTGTCACA ATTGATAGAA GAGATGAAGA AGGAAAAACT 
GCATTTGATA TAGCATGTGA GAATGATCAT AAGGATGTGG CACGAGCATT TCTAGAAACG 
GATCAATGGA AAAACTTGAT GATTCCATGT GATGTGATTC CATTGGATAA GCACAGAAAT 
CCTGTAAATA TGAAAAGGAG AACACCCTTC AGAACTCTTC TGACAAAGTT TCCAGAACTA 
GCTTCGTTTG TTATGGACAA CTGTATAGAA AAAAGCAAAG AAGAAACCGA TTCAACTCAA 
AGTGTTGCAT ACAATTTTGA ATTTCTCGAT GATACTTACA TGATGAGATG TGTGAGTGAT 
GATGGAACAG GAGAACAATT AATTGGATGC AAATCTGCAT ATGATGAAGA TTTCAAACTG 
GAAAAGGATG CACAATCCTA TGCTTCCAAT TACGACCGCG TCTATAAATA CCATCCATTG 
AAGCTAATGG CAGACGCTGA AAAACTGCAT CTTCTCAACC ATCCACTATC AAAAGCCCTT 
CTCAAATACA AATGGAATCG GCTGGGGAGA CCAATGTATT ATTTTGCTCT TTTCATGTAT 
CTTGTTTTTA TTGTCAGTTT GACTCAATAT GTAAGACATA CAAAGGCTCC ATACAATGTT 
TGGAATGAGG AATCGTATTA TGACAGTGAA TACTTCGATG AAAACGAGAC GTGCCCTCAG 
ATTAACACCA CAAAACCTGA CGTCGTATGG AAAATCATCA TTCAAACTTT AGCCGTCTGT 
CAAATTCTAG TGGAATGTTT CCAATTATTC CAACGAAAAT TTGCATATCT GGTAAACTGG 
GAGAACTGGA TTGATTGTTT TATATACTCA ACAGCCCTTA TTACTGTATA CGACTTCTCC 
GAATGCTCAG CAACATCAGG AGTTCGTCAG AACTGGCAAT GGATTCTAGC AGCTTTATGC 
ATATTTTTCG GATGGATCAA CTTATTGTTC ATGATCAGAA AAATGCCAAG ATTTGGAATA 
TTCGTGGTGA TGTTTGTGGA TATTGTGAAG ACATTCTTCC GCTTCTTCCC AGTTTTTGTG 
CTATTCATAA TTGCGTTCTC TTCATCCTTC TACGTGATCC TTCAAAATAG ACCGGAATTT 
TCCACGATCT TCATGTCTCC TCTCAAAACT ACAGTAATGA TGATTGGAGA ATTTGAGTTC 
ACTGGAATAT TTCACGGAGA TGAAACTACT CATGCTGAGA AAATGTTTGG CCCAGCACAC 
ACAGCAGTCG CATGTGCACT TTTCTTCTTT TTCTGCATAA TAATGACAAT TCTCCTGATG 
AATCTACTTG TTGGTTTGGC AGTTGATGAT ATTAAAGGTG TACAAGAAAA AGCTGAACTG 
AAACGTCTCG CAATGCAAGT TGATCTTGTT CTGCAAATTG AGGCCTCTCT TCACTTTTTC 
ATTCAACGAA CCAAAAAATA TGCGACTTGC CGTTATGCAA CTTTTCCATA CGGAAAACTT 
CATAAGACTG GTTTTGCCGG ATGGTGGTCC AATTTCAGAA GAAGATTCGG GTTGAGTGTC 
AGCACTGATC CAGAAATTGA CGAGATGTAT GAACGGGAAG CGGAGTTCAC TTCTGAAATG 
ACTCAAAAAC TTCAAAACCA AGCAGCTAAA CTCAAAAATA TTCAAGAAAA TATTGATGTG 
ATGTACGAGA AGCAAGTACG ACTTGAAGCA ATCATTGCAA AGCTCGCGAC AGGGCTCAAC 
ATAAATATTG AACTTGAGGA GAAAGATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294083.1
CDS	1..3633
Translation	MSKKSLGLDVRLELEGLISNDDTIRSENGRQASIFRVAELDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTKTVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCIEKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTKPDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIYSTALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQRTKKYATCRYATFPYGKLHKTGFAGWWSNFRRRFGLSVSTDPEIDEMYEREAEFTSEMTQKLQNQAAKLKNIQENIDVMYEKQVRLEAIIAKLATGLNINIELEEKDN

Target ORF information:

RefSeq Version	NM_001307154.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ANK_REP_REGION domain-containing protein (trpa-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307154.2

ORF Insert Sequence:

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAATGGTCGT CAAGCTTCAA TCTTTCGAGT CGCTGAACTA 
GATGCTCGCA CTGAAGCAGA CAATCTTCGT TCAATAATCC ATCAAAGTGC TCGAGAAGGA 
AATGTAAACG CTCTTCAAGA AGCTCTGCTG AAAGCTCCAT TGGCAGTGAA CGCCCAAGAT 
GGTGATTTTA TGACACCGCT TCACTATGCA GCGAGATACG GAAACTATGA TGCAGTTAAA 
CTATTACTTT CTAAAAATGC TCTACCTAAT ACAAAAAATC GAGAAGGCGA CACTCCGTTG 
CATATTGCTT CCAAGTATAT CTATGGATAC AGTGATATTT GCTCAATTAT AGATGAGGAT 
CAAGCTGATT CCGCGAGAAA GTACAATACT GCAACCAAGA AGATCATAAA TGCCTTAGTT 
TCTGAAAATG CTGAAATCGA TCCTGTGAAT AAGTACCAAT TGACTCCTCT TCACTATGCA 
GCCATGAAAT CCAACTTTTC TGCTCTACAC GCTCTCATAA AACTGAAAGC AGATGTGGAT 
GCGGAAGATG ATAACAAAAT GACACCTCTT CTATTAGCCT GTGTTCATGG AAGCCAAGAA 
ATAATTCAAG AGCTTATTAA AGCAAATAGT AATGTTACGA AAAGAGATCA ACGACTCAAC 
ACAGTATTTC ATATTGTTGC ATTGCGGGGT GAGCCGGAAT ACTTAGAGAT GATGATGGAT 
CATGATCCAG TTGAAGCAAT CAAGGCATTA AATTTATTTA ATAATGAAAA GAAGACACCT 
TTGAGAATGG CTGTAGAAGG AAATCATCCG GAAACTTTGA AAAAGATTCT GCAGATGGAA 
AAGAAAAATT CATGTAAATG GATGGATAGG GAGAAGGAAT TGATCCATTT TGCCGCTGAA 
AAGGGATTTT TAGAAGTTTT AAAAGCATTA GTGGAAGCTG GAGGAAACAA GAATGAACTG 
AATGAAGTTA AAGCAGTGCC ACTTCACGTT GCTGCTCAAA TGAATCAATT GGAAGTTGTA 
AGCTATTTAA TAGAAGAGGA AAAGGATAAT ATTGATGTAG TCGATGAACA AGGCTTGACT 
CCCTTGATGA TGGCAGTCAC TCATGATAGT AAAAAGTGTG TTGAGTACCT GATTGCAAAA 
AAAGCAAACT TGACAATCAC GGATAAAGAT GAACGTACCC CAGTATTCAT TGGAGCCAAG 
TTCAATGCTC TTTCTTCTGT TGAATACATT TTGGACCACC TTAGAAAAAA AAATAAAGAG 
ACTGAAAGAA GTGCTTTAAA GTCACCTACT CGAAATACAC TTCGGATTGT GTCTGAAGAC 
GTGAGGAGGA CTATGGTCAA TATGGTAGAT CGAGATCAAA ACACTCCAAT GCATATTGTT 
GCTTCAAATG GATATTTGGA AATGATGCAG TTGCTCCAAA AACACGGAGC ATCAATTACT 
CAAGTGAACG AAGATGAGGA GACTGCATTG CACAGAGCTG CAATTGGGGG TCAAACTGGA 
GCAGTAAGAC AACTACTTGA ATGGGATATT CGACTTTTGT TGATGAAAGA TGAAATGGGA 
AATAGTGCAT TGCATTTGGC AGCCAGAAGT GGACACGATG CCACAACAAA AGTTTTATTG 
GACAACGGTG CTGATAAGGA AGCAAAGAAT TCGTACCAAA AAACTCCGTT ACAAGTAGCC 
GTGGATTCTG GAAAATTGGA AACCTGTCAG AGATTAGTGG CAAAGGGAGC ACAGATTGAG 
AGCTCTAGTG ATACAAAAAC AGTATTGCAT ACAGCTGCAT TCTATGGTAA TGAATCTATC 
GTTAGATACT TTATCGCTGA GGGTGTCACA ATTGATAGAA GAGATGAAGA AGGAAAAACT 
GCATTTGATA TAGCATGTGA GAATGATCAT AAGGATGTGG CACGAGCATT TCTAGAAACG 
GATCAATGGA AAAACTTGAT GATTCCATGT GATGTGATTC CATTGGATAA GCACAGAAAT 
CCTGTAAATA TGAAAAGGAG AACACCCTTC AGAACTCTTC TGACAAAGTT TCCAGAACTA 
GCTTCGTTTG TTATGGACAA CTGTATAGAA AAAAGCAAAG AAGAAACCGA TTCAACTCAA 
AGTGTTGCAT ACAATTTTGA ATTTCTCGAT GATACTTACA TGATGAGATG TGTGAGTGAT 
GATGGAACAG GAGAACAATT AATTGGATGC AAATCTGCAT ATGATGAAGA TTTCAAACTG 
GAAAAGGATG CACAATCCTA TGCTTCCAAT TACGACCGCG TCTATAAATA CCATCCATTG 
AAGCTAATGG CAGACGCTGA AAAACTGCAT CTTCTCAACC ATCCACTATC AAAAGCCCTT 
CTCAAATACA AATGGAATCG GCTGGGGAGA CCAATGTATT ATTTTGCTCT TTTCATGTAT 
CTTGTTTTTA TTGTCAGTTT GACTCAATAT GTAAGACATA CAAAGGCTCC ATACAATGTT 
TGGAATGAGG AATCGTATTA TGACAGTGAA TACTTCGATG AAAACGAGAC GTGCCCTCAG 
ATTAACACCA CAAAACCTGA CGTCGTATGG AAAATCATCA TTCAAACTTT AGCCGTCTGT 
CAAATTCTAG TGGAATGTTT CCAATTATTC CAACGAAAAT TTGCATATCT GGTAAACTGG 
GAGAACTGGA TTGATTGTTT TATATACTCA ACAGCCCTTA TTACTGTATA CGACTTCTCC 
GAATGCTCAG CAACATCAGG AGTTCGTCAG AACTGGCAAT GGATTCTAGC AGCTTTATGC 
ATATTTTTCG GATGGATCAA CTTATTGTTC ATGATCAGAA AAATGCCAAG ATTTGGAATA 
TTCGTGGTGA TGTTTGTGGA TATTGTGAAG ACATTCTTCC GCTTCTTCCC AGTTTTTGTG 
CTATTCATAA TTGCGTTCTC TTCATCCTTC TACGTGATCC TTCAAAATAG ACCGGAATTT 
TCCACGATCT TCATGTCTCC TCTCAAAACT ACAGTAATGA TGATTGGAGA ATTTGAGTTC 
ACTGGAATAT TTCACGGAGA TGAAACTACT CATGCTGAGA AAATGTTTGG CCCAGCACAC 
ACAGCAGTCG CATGTGCACT TTTCTTCTTT TTCTGCATAA TAATGACAAT TCTCCTGATG 
AATCTACTTG TTGGTTTGGC AGTTGATGAT ATTAAAGGTG TACAAGAAAA AGCTGAACTG 
AAACGTCTCG CAATGCAAGT TGATCTTGTT CTGCAAATTG AGGCCTCTCT TCACTTTTTC 
ATTCAACGAA CCAAAAAATA TGCGACTTGC CGTTATGCAA CTTTTCCATA CGGAAAACTT 
CATAAGACTG GTTTTGCCGG ATGGTGGTCC AATTTCAGAA GAAGATTCGG GTTGAGTGTC 
AGCACTGATC CAGAAATTGA CGAGATGTAT GAACGGGAAG CGGAGTTCAC TTCTGAAATG 
ACTCAAAAAC TTCAAAACCA AGCAGCTAAA CTCAAAAATA TTCAAGAAAA TATTGATGTG 
ATGTACGAGA AGCAAGTACG ACTTGAAGCA ATCATTGCAA AGCTCGCGAC AGGGCTCAAC 
ATAAATATTG AACTTGAGGA GAAAGATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpa-1 ( NM_001307154.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294083.1 Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), partial mRNA.

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CloneID	OCa343125
Clone ID Related Accession (Same CDS sequence)	NM_001307154.2 , NM_001307154.1
Accession Version	NM_001307154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Transient receptor potential cation channel subfamily A member 1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAATGGTCGT CAAGCTTCAA TCTTTCGAGT CGCTGAACTA 
GATGCTCGCA CTGAAGCAGA CAATCTTCGT TCAATAATCC ATCAAAGTGC TCGAGAAGGA 
AATGTAAACG CTCTTCAAGA AGCTCTGCTG AAAGCTCCAT TGGCAGTGAA CGCCCAAGAT 
GGTGATTTTA TGACACCGCT TCACTATGCA GCGAGATACG GAAACTATGA TGCAGTTAAA 
CTATTACTTT CTAAAAATGC TCTACCTAAT ACAAAAAATC GAGAAGGCGA CACTCCGTTG 
CATATTGCTT CCAAGTATAT CTATGGATAC AGTGATATTT GCTCAATTAT AGATGAGGAT 
CAAGCTGATT CCGCGAGAAA GTACAATACT GCAACCAAGA AGATCATAAA TGCCTTAGTT 
TCTGAAAATG CTGAAATCGA TCCTGTGAAT AAGTACCAAT TGACTCCTCT TCACTATGCA 
GCCATGAAAT CCAACTTTTC TGCTCTACAC GCTCTCATAA AACTGAAAGC AGATGTGGAT 
GCGGAAGATG ATAACAAAAT GACACCTCTT CTATTAGCCT GTGTTCATGG AAGCCAAGAA 
ATAATTCAAG AGCTTATTAA AGCAAATAGT AATGTTACGA AAAGAGATCA ACGACTCAAC 
ACAGTATTTC ATATTGTTGC ATTGCGGGGT GAGCCGGAAT ACTTAGAGAT GATGATGGAT 
CATGATCCAG TTGAAGCAAT CAAGGCATTA AATTTATTTA ATAATGAAAA GAAGACACCT 
TTGAGAATGG CTGTAGAAGG AAATCATCCG GAAACTTTGA AAAAGATTCT GCAGATGGAA 
AAGAAAAATT CATGTAAATG GATGGATAGG GAGAAGGAAT TGATCCATTT TGCCGCTGAA 
AAGGGATTTT TAGAAGTTTT AAAAGCATTA GTGGAAGCTG GAGGAAACAA GAATGAACTG 
AATGAAGTTA AAGCAGTGCC ACTTCACGTT GCTGCTCAAA TGAATCAATT GGAAGTTGTA 
AGCTATTTAA TAGAAGAGGA AAAGGATAAT ATTGATGTAG TCGATGAACA AGGCTTGACT 
CCCTTGATGA TGGCAGTCAC TCATGATAGT AAAAAGTGTG TTGAGTACCT GATTGCAAAA 
AAAGCAAACT TGACAATCAC GGATAAAGAT GAACGTACCC CAGTATTCAT TGGAGCCAAG 
TTCAATGCTC TTTCTTCTGT TGAATACATT TTGGACCACC TTAGAAAAAA AAATAAAGAG 
ACTGAAAGAA GTGCTTTAAA GTCACCTACT CGAAATACAC TTCGGATTGT GTCTGAAGAC 
GTGAGGAGGA CTATGGTCAA TATGGTAGAT CGAGATCAAA ACACTCCAAT GCATATTGTT 
GCTTCAAATG GATATTTGGA AATGATGCAG TTGCTCCAAA AACACGGAGC ATCAATTACT 
CAAGTGAACG AAGATGAGGA GACTGCATTG CACAGAGCTG CAATTGGGGG TCAAACTGGA 
GCAGTAAGAC AACTACTTGA ATGGGATATT CGACTTTTGT TGATGAAAGA TGAAATGGGA 
AATAGTGCAT TGCATTTGGC AGCCAGAAGT GGACACGATG CCACAACAAA AGTTTTATTG 
GACAACGGTG CTGATAAGGA AGCAAAGAAT TCGTACCAAA AAACTCCGTT ACAAGTAGCC 
GTGGATTCTG GAAAATTGGA AACCTGTCAG AGATTAGTGG CAAAGGGAGC ACAGATTGAG 
AGCTCTAGTG ATACAAAAAC AGTATTGCAT ACAGCTGCAT TCTATGGTAA TGAATCTATC 
GTTAGATACT TTATCGCTGA GGGTGTCACA ATTGATAGAA GAGATGAAGA AGGAAAAACT 
GCATTTGATA TAGCATGTGA GAATGATCAT AAGGATGTGG CACGAGCATT TCTAGAAACG 
GATCAATGGA AAAACTTGAT GATTCCATGT GATGTGATTC CATTGGATAA GCACAGAAAT 
CCTGTAAATA TGAAAAGGAG AACACCCTTC AGAACTCTTC TGACAAAGTT TCCAGAACTA 
GCTTCGTTTG TTATGGACAA CTGTATAGAA AAAAGCAAAG AAGAAACCGA TTCAACTCAA 
AGTGTTGCAT ACAATTTTGA ATTTCTCGAT GATACTTACA TGATGAGATG TGTGAGTGAT 
GATGGAACAG GAGAACAATT AATTGGATGC AAATCTGCAT ATGATGAAGA TTTCAAACTG 
GAAAAGGATG CACAATCCTA TGCTTCCAAT TACGACCGCG TCTATAAATA CCATCCATTG 
AAGCTAATGG CAGACGCTGA AAAACTGCAT CTTCTCAACC ATCCACTATC AAAAGCCCTT 
CTCAAATACA AATGGAATCG GCTGGGGAGA CCAATGTATT ATTTTGCTCT TTTCATGTAT 
CTTGTTTTTA TTGTCAGTTT GACTCAATAT GTAAGACATA CAAAGGCTCC ATACAATGTT 
TGGAATGAGG AATCGTATTA TGACAGTGAA TACTTCGATG AAAACGAGAC GTGCCCTCAG 
ATTAACACCA CAAAACCTGA CGTCGTATGG AAAATCATCA TTCAAACTTT AGCCGTCTGT 
CAAATTCTAG TGGAATGTTT CCAATTATTC CAACGAAAAT TTGCATATCT GGTAAACTGG 
GAGAACTGGA TTGATTGTTT TATATACTCA ACAGCCCTTA TTACTGTATA CGACTTCTCC 
GAATGCTCAG CAACATCAGG AGTTCGTCAG AACTGGCAAT GGATTCTAGC AGCTTTATGC 
ATATTTTTCG GATGGATCAA CTTATTGTTC ATGATCAGAA AAATGCCAAG ATTTGGAATA 
TTCGTGGTGA TGTTTGTGGA TATTGTGAAG ACATTCTTCC GCTTCTTCCC AGTTTTTGTG 
CTATTCATAA TTGCGTTCTC TTCATCCTTC TACGTGATCC TTCAAAATAG ACCGGAATTT 
TCCACGATCT TCATGTCTCC TCTCAAAACT ACAGTAATGA TGATTGGAGA ATTTGAGTTC 
ACTGGAATAT TTCACGGAGA TGAAACTACT CATGCTGAGA AAATGTTTGG CCCAGCACAC 
ACAGCAGTCG CATGTGCACT TTTCTTCTTT TTCTGCATAA TAATGACAAT TCTCCTGATG 
AATCTACTTG TTGGTTTGGC AGTTGATGAT ATTAAAGGTG TACAAGAAAA AGCTGAACTG 
AAACGTCTCG CAATGCAAGT TGATCTTGTT CTGCAAATTG AGGCCTCTCT TCACTTTTTC 
ATTCAACGAA CCAAAAAATA TGCGACTTGC CGTTATGCAA CTTTTCCATA CGGAAAACTT 
CATAAGACTG GTTTTGCCGG ATGGTGGTCC AATTTCAGAA GAAGATTCGG GTTGAGTGTC 
AGCACTGATC CAGAAATTGA CGAGATGTAT GAACGGGAAG CGGAGTTCAC TTCTGAAATG 
ACTCAAAAAC TTCAAAACCA AGCAGCTAAA CTCAAAAATA TTCAAGAAAA TATTGATGTG 
ATGTACGAGA AGCAAGTACG ACTTGAAGCA ATCATTGCAA AGCTCGCGAC AGGGCTCAAC 
ATAAATATTG AACTTGAGGA GAAAGATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294083.1
CDS	1..3633
Translation	MSKKSLGLDVRLELEGLISNDDTIRSENGRQASIFRVAELDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTKTVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCIEKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTKPDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIYSTALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQRTKKYATCRYATFPYGKLHKTGFAGWWSNFRRRFGLSVSTDPEIDEMYEREAEFTSEMTQKLQNQAAKLKNIQENIDVMYEKQVRLEAIIAKLATGLNINIELEEKDN

Target ORF information:

RefSeq Version	NM_001307154.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transient receptor potential cation channel subfamily A member 1 homolog (trpa-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307154.1

ORF Insert Sequence:

ATGTCGAAGA AATCATTAGG GCTTGATGTT CGGCTGGAAT TGGAAGGATT GATTTCAAAT 
GATGATACAA TCAGATCTGA GAATGGTCGT CAAGCTTCAA TCTTTCGAGT CGCTGAACTA 
GATGCTCGCA CTGAAGCAGA CAATCTTCGT TCAATAATCC ATCAAAGTGC TCGAGAAGGA 
AATGTAAACG CTCTTCAAGA AGCTCTGCTG AAAGCTCCAT TGGCAGTGAA CGCCCAAGAT 
GGTGATTTTA TGACACCGCT TCACTATGCA GCGAGATACG GAAACTATGA TGCAGTTAAA 
CTATTACTTT CTAAAAATGC TCTACCTAAT ACAAAAAATC GAGAAGGCGA CACTCCGTTG 
CATATTGCTT CCAAGTATAT CTATGGATAC AGTGATATTT GCTCAATTAT AGATGAGGAT 
CAAGCTGATT CCGCGAGAAA GTACAATACT GCAACCAAGA AGATCATAAA TGCCTTAGTT 
TCTGAAAATG CTGAAATCGA TCCTGTGAAT AAGTACCAAT TGACTCCTCT TCACTATGCA 
GCCATGAAAT CCAACTTTTC TGCTCTACAC GCTCTCATAA AACTGAAAGC AGATGTGGAT 
GCGGAAGATG ATAACAAAAT GACACCTCTT CTATTAGCCT GTGTTCATGG AAGCCAAGAA 
ATAATTCAAG AGCTTATTAA AGCAAATAGT AATGTTACGA AAAGAGATCA ACGACTCAAC 
ACAGTATTTC ATATTGTTGC ATTGCGGGGT GAGCCGGAAT ACTTAGAGAT GATGATGGAT 
CATGATCCAG TTGAAGCAAT CAAGGCATTA AATTTATTTA ATAATGAAAA GAAGACACCT 
TTGAGAATGG CTGTAGAAGG AAATCATCCG GAAACTTTGA AAAAGATTCT GCAGATGGAA 
AAGAAAAATT CATGTAAATG GATGGATAGG GAGAAGGAAT TGATCCATTT TGCCGCTGAA 
AAGGGATTTT TAGAAGTTTT AAAAGCATTA GTGGAAGCTG GAGGAAACAA GAATGAACTG 
AATGAAGTTA AAGCAGTGCC ACTTCACGTT GCTGCTCAAA TGAATCAATT GGAAGTTGTA 
AGCTATTTAA TAGAAGAGGA AAAGGATAAT ATTGATGTAG TCGATGAACA AGGCTTGACT 
CCCTTGATGA TGGCAGTCAC TCATGATAGT AAAAAGTGTG TTGAGTACCT GATTGCAAAA 
AAAGCAAACT TGACAATCAC GGATAAAGAT GAACGTACCC CAGTATTCAT TGGAGCCAAG 
TTCAATGCTC TTTCTTCTGT TGAATACATT TTGGACCACC TTAGAAAAAA AAATAAAGAG 
ACTGAAAGAA GTGCTTTAAA GTCACCTACT CGAAATACAC TTCGGATTGT GTCTGAAGAC 
GTGAGGAGGA CTATGGTCAA TATGGTAGAT CGAGATCAAA ACACTCCAAT GCATATTGTT 
GCTTCAAATG GATATTTGGA AATGATGCAG TTGCTCCAAA AACACGGAGC ATCAATTACT 
CAAGTGAACG AAGATGAGGA GACTGCATTG CACAGAGCTG CAATTGGGGG TCAAACTGGA 
GCAGTAAGAC AACTACTTGA ATGGGATATT CGACTTTTGT TGATGAAAGA TGAAATGGGA 
AATAGTGCAT TGCATTTGGC AGCCAGAAGT GGACACGATG CCACAACAAA AGTTTTATTG 
GACAACGGTG CTGATAAGGA AGCAAAGAAT TCGTACCAAA AAACTCCGTT ACAAGTAGCC 
GTGGATTCTG GAAAATTGGA AACCTGTCAG AGATTAGTGG CAAAGGGAGC ACAGATTGAG 
AGCTCTAGTG ATACAAAAAC AGTATTGCAT ACAGCTGCAT TCTATGGTAA TGAATCTATC 
GTTAGATACT TTATCGCTGA GGGTGTCACA ATTGATAGAA GAGATGAAGA AGGAAAAACT 
GCATTTGATA TAGCATGTGA GAATGATCAT AAGGATGTGG CACGAGCATT TCTAGAAACG 
GATCAATGGA AAAACTTGAT GATTCCATGT GATGTGATTC CATTGGATAA GCACAGAAAT 
CCTGTAAATA TGAAAAGGAG AACACCCTTC AGAACTCTTC TGACAAAGTT TCCAGAACTA 
GCTTCGTTTG TTATGGACAA CTGTATAGAA AAAAGCAAAG AAGAAACCGA TTCAACTCAA 
AGTGTTGCAT ACAATTTTGA ATTTCTCGAT GATACTTACA TGATGAGATG TGTGAGTGAT 
GATGGAACAG GAGAACAATT AATTGGATGC AAATCTGCAT ATGATGAAGA TTTCAAACTG 
GAAAAGGATG CACAATCCTA TGCTTCCAAT TACGACCGCG TCTATAAATA CCATCCATTG 
AAGCTAATGG CAGACGCTGA AAAACTGCAT CTTCTCAACC ATCCACTATC AAAAGCCCTT 
CTCAAATACA AATGGAATCG GCTGGGGAGA CCAATGTATT ATTTTGCTCT TTTCATGTAT 
CTTGTTTTTA TTGTCAGTTT GACTCAATAT GTAAGACATA CAAAGGCTCC ATACAATGTT 
TGGAATGAGG AATCGTATTA TGACAGTGAA TACTTCGATG AAAACGAGAC GTGCCCTCAG 
ATTAACACCA CAAAACCTGA CGTCGTATGG AAAATCATCA TTCAAACTTT AGCCGTCTGT 
CAAATTCTAG TGGAATGTTT CCAATTATTC CAACGAAAAT TTGCATATCT GGTAAACTGG 
GAGAACTGGA TTGATTGTTT TATATACTCA ACAGCCCTTA TTACTGTATA CGACTTCTCC 
GAATGCTCAG CAACATCAGG AGTTCGTCAG AACTGGCAAT GGATTCTAGC AGCTTTATGC 
ATATTTTTCG GATGGATCAA CTTATTGTTC ATGATCAGAA AAATGCCAAG ATTTGGAATA 
TTCGTGGTGA TGTTTGTGGA TATTGTGAAG ACATTCTTCC GCTTCTTCCC AGTTTTTGTG 
CTATTCATAA TTGCGTTCTC TTCATCCTTC TACGTGATCC TTCAAAATAG ACCGGAATTT 
TCCACGATCT TCATGTCTCC TCTCAAAACT ACAGTAATGA TGATTGGAGA ATTTGAGTTC 
ACTGGAATAT TTCACGGAGA TGAAACTACT CATGCTGAGA AAATGTTTGG CCCAGCACAC 
ACAGCAGTCG CATGTGCACT TTTCTTCTTT TTCTGCATAA TAATGACAAT TCTCCTGATG 
AATCTACTTG TTGGTTTGGC AGTTGATGAT ATTAAAGGTG TACAAGAAAA AGCTGAACTG 
AAACGTCTCG CAATGCAAGT TGATCTTGTT CTGCAAATTG AGGCCTCTCT TCACTTTTTC 
ATTCAACGAA CCAAAAAATA TGCGACTTGC CGTTATGCAA CTTTTCCATA CGGAAAACTT 
CATAAGACTG GTTTTGCCGG ATGGTGGTCC AATTTCAGAA GAAGATTCGG GTTGAGTGTC 
AGCACTGATC CAGAAATTGA CGAGATGTAT GAACGGGAAG CGGAGTTCAC TTCTGAAATG 
ACTCAAAAAC TTCAAAACCA AGCAGCTAAA CTCAAAAATA TTCAAGAAAA TATTGATGTG 
ATGTACGAGA AGCAAGTACG ACTTGAAGCA ATCATTGCAA AGCTCGCGAC AGGGCTCAAC 
ATAAATATTG AACTTGAGGA GAAAGATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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trpa-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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